MD15G1258300.v1.1

Transmembrane protein 53

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014260
Physical Location & Seq
Forward (+)
21949770 .. 21951961
2192 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1258300.v1.1.491

Sequence Viewer

Length: 1188 bp
ATGGAAGCTCCGGCGAGAATTCTCAGTCTCAATCGCCAGCTCTGTACCAAAACGACGCCGTCCGCCCTCGCTTTCTCCCCAAAACTCCGTCGGCCCGCAAATTCCTCGCTTATATTTCACACTCTCGCTCGAAACGGCGTCGGCAAAAGAACCCCCTTTTCTCTTTCTCTCGCGCAGTCGCACTCCTCGTTCTGCTTCAATCCGAACCCCTTCAAATCCCTCCCCCTTTCTCTCTCCTCCCAGTCGCCGTTCAGCAGCTTCAACCCCTCTCTCTCACCCTCAAACGACGACGTTTCCACCCCGAATCGGGCGTTCCACGGTGGCATTGTCGGAAATGTGGGTTCTTTCGGGACCCACAACCTTGAAGTGAGAGTGGTGTTGCTGGGCTGGCTCGGCGCCAAGACGAAGCATCTGAAGAGGTACGTGGAATGGTACAACTCCAGAGGCATTCACGCCGTGACCTTCGTCGTTGATCCGAGGGAGGTGCTCTGGTTTGATTTGGGCCGCCGGGTAGAAAATCGGGTTTCAGACTTGGCCCGGGAGCTCGCTTCGTGGGCGGAGCATGGCGGAGAAAGGTGTTTGCTTTTTCACACGTTCAGCAATACGGGTTGGTATGGTGCCATTCTTGAAATTCTGCAGCGGAGGCAAGACCTGATGGAGAAGATTAAAGGGTGTGTGGTTGATTCAGGAGCAGCTGAACCTTTTAATCCTAAGGTGTGGGCAGCAGGATTTGCTGCTGCGATTTTGAAAAAACGCAGCTCTTTAACAAGCGCTGCATTTGAGGCTAGAGAATTAAATGAGTCGGAAGATGCAGCAAGCTTGTCAAAGCTGCAAGAAGAGAAGCCTCCACTTTCCGAAGCCGTGGTTTTACTGATATTGGAGAAGCTGTTTTCAGGTCTTCTAAAGTTTCCTGATGTGGATAAGAGGTTAACGAAGGTAGTTTCTATCCTTTCGGAAAACAGTCCTGATTGCCCACAGCTTTACCTATACAGTACTGCTGATAAAGTCGTTCCACACCAGTCAATTGAGTCGTTCATGGCTGAGCAAAAGAGGAAGGGTAGAATTGTACGGTCCTTCAATTTCGGCACAACACCTCATGTGGATCACTTCAGGAATTTCCCCGACATATACTCTTCGGAGCTCGACAGTTTCTTGAAAGATTGTTTAACAACAGTTAAGATGATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF829

Protein Analysis

396

Amino Acids

44.01

Weight (kDa)

9.23

Isoelectric Point (pI)

48.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF829 PF05705 125 - 385 2.6e-57 Eukaryotic protein of unknown function (DUF829)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014260)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18245
fragaria_vesca FvH4_1g13010
malus_domestica MD02G1144600.v1.1 MD15G1258300.v1.1
prunus_persica Prupe.7G157000_v2.0.a1 Prupe.7G157000_v2.0.a1
pyrus_communis pycom02g11450 pycom15g22610
rosa_chinensis RchiOBHm_Chr2g0101161
rosa_laevigata RLG00000017051
rosa_roxburghii Rroxscaffold_2G00141530
rosa_rugosa Rorug02G0100600
rosa_samantha Rh2AG144000 Rh2BG149000 Rh2CG150300 Rh2DG149500
rosa_wichuraiana Rw2G011290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 395, 617
AccII CGCG 1 cut(s) 173
AciI CCGC 6 cut(s) 63, 96, 505, 557, 567, 640
AclWI GGATC 2 cut(s) 467, 1110
AcsI RAATTY 4 cut(s) 18, 100, 630, 1114
AcuI CTGAAG 2 cut(s) 434, 1093
AcyI GRCGYC 3 cut(s) 56, 138, 396
AdeI CACNNNGTG 1 cut(s) 457
AfaI GTAC 5 cut(s) 46, 422, 434, 994, 1068
AfeI AGCGCT 1 cut(s) 772
AfiI CCNNNNNNNGG 2 cut(s) 306, 307
AflIII ACRYGT 1 cut(s) 591
AgsI TTSAA 8 cut(s) 199, 214, 262, 365, 629, 748, 1078, 1156
Alw21I GWGCWC 3 cut(s) 489, 546, 1143
Alw26I GTCTC 1 cut(s) 32
AlwI GGATC 2 cut(s) 467, 1110
Ama87I CYCGRG 1 cut(s) 537
Aor51HI AGCGCT 1 cut(s) 772
AoxI GGCC 3 cut(s) 92, 502, 534
ApoI RAATTY 4 cut(s) 18, 100, 630, 1114
Asp700I GAANNNNTTC 1 cut(s) 209
AspLEI GCGC 3 cut(s) 175, 398, 773
AspS9I GGNCC 5 cut(s) 93, 351, 502, 535, 1071
AsuC2I CCSGG 3 cut(s) 509, 538, 539
AsuHPI GGTGA 1 cut(s) 267
AvaI CYCGRG 1 cut(s) 537
AvaII GGWCC 2 cut(s) 351, 1071
AxyI CCTNAGG 1 cut(s) 711
BanI GGYRCC 2 cut(s) 395, 617
BanII GRGCYC 2 cut(s) 546, 1143
BbsI GAAGAC 1 cut(s) 890
Bbv12I GWGCWC 3 cut(s) 489, 546, 1143
BccI CCATC 1 cut(s) 649
BceAI ACGGC 5 cut(s) 43, 151, 232, 440, 845
BcgI CGANNNNNNTGC 4 cut(s) 87, 121, 466, 500
BcnI CCSGG 3 cut(s) 509, 538, 539
BcoDI GTCTC 1 cut(s) 32
BfaI CTAG 1 cut(s) 786
BfmI CTRYAG 1 cut(s) 635
BfoI RGCGCY 2 cut(s) 399, 774
BlpI GCTNAGC 1 cut(s) 1041
BmcAI AGTACT 1 cut(s) 994
Bme1390I CCNGG 3 cut(s) 509, 538, 539
Bme18I GGWCC 2 cut(s) 351, 1071
BmeT110I CYCGRG 1 cut(s) 537
BmgT120I GGNCC 5 cut(s) 93, 351, 502, 535, 1071
BmiI GGNNCC 4 cut(s) 352, 353, 397, 619
BmrFI CCNGG 3 cut(s) 509, 538, 539
BmrI ACTGGG 1 cut(s) 235
BmsI GCATC 2 cut(s) 418, 799
BmuI ACTGGG 1 cut(s) 235
BoxI GACNNNNGTC 1 cut(s) 464
BpiI GAAGAC 1 cut(s) 890
BpmI CTGGAG 1 cut(s) 424
Bpu1102I GCTNAGC 1 cut(s) 1041
BpuMI CCSGG 3 cut(s) 509, 538, 539
BsaAI YACGTR 1 cut(s) 424
BsaHI GRCGYC 3 cut(s) 56, 138, 396
BsaJI CCNNGG 4 cut(s) 316, 476, 537, 861
BsaXI ACNNNNNCTCC 2 cut(s) 872, 902
Bsc4I CCNNNNNNNGG 2 cut(s) 306, 307
Bse1I ACTGG 2 cut(s) 241, 1018
Bse21I CCTNAGG 1 cut(s) 711
BseDI CCNNGG 4 cut(s) 316, 476, 537, 861
BseLI CCNNNNNNNGG 2 cut(s) 306, 307
BseMII CTCAG 2 cut(s) 37, 1032
BseNI ACTGG 2 cut(s) 241, 1018
BseRI GAGGAG 2 cut(s) 175, 226
BseYI CCCAGC 1 cut(s) 382
Bsh1236I CGCG 1 cut(s) 173
BshFI GGCC 3 cut(s) 94, 504, 536
BshNI GGYRCC 2 cut(s) 395, 617
BsiHKAI GWGCWC 3 cut(s) 489, 546, 1143
BsiHKCI CYCGRG 1 cut(s) 537
BsiSI CCGG 3 cut(s) 11, 508, 538
BslFI GGGAC 1 cut(s) 364
BslI CCNNNNNNNGG 2 cut(s) 306, 307
BsmAI GTCTC 1 cut(s) 32
BsmFI GGGAC 1 cut(s) 364
BsmI GAATGC 1 cut(s) 447
BsnI GGCC 3 cut(s) 94, 504, 536
BsoBI CYCGRG 1 cut(s) 537
Bsp1286I GDGCHC 3 cut(s) 489, 546, 1143
Bsp143I GATC 2 cut(s) 472, 1102
Bsp1720I GCTNAGC 1 cut(s) 1041
BspACI CCGC 6 cut(s) 63, 96, 505, 557, 567, 640
BspANI GGCC 3 cut(s) 94, 504, 536
BspCNI CTCAG 2 cut(s) 36, 1033
BspFNI CGCG 1 cut(s) 173
BspLI GGNNCC 4 cut(s) 352, 353, 397, 619
BspMAI CTGCAG 1 cut(s) 639
BspPI GGATC 2 cut(s) 467, 1110
BspT107I GGYRCC 2 cut(s) 395, 617
BsrI ACTGG 2 cut(s) 241, 1018
BssECI CCNNGG 4 cut(s) 316, 476, 537, 861
BssMI GATC 2 cut(s) 472, 1102
BssNI GRCGYC 3 cut(s) 56, 138, 396
Bst4CI ACNGT 6 cut(s) 320, 962, 992, 1071, 1148, 1174
Bst6I CTCTTC 3 cut(s) 410, 831, 1138
BstACI GRCGYC 3 cut(s) 56, 138, 396
BstAPI GCANNNNNTGC 1 cut(s) 731
BstBAI YACGTR 1 cut(s) 424
BstC8I GCNNGC 5 cut(s) 38, 96, 389, 546, 817
BstDEI CTNAG 3 cut(s) 23, 711, 1041
BstDSI CCRYGG 2 cut(s) 316, 861
BstFNI CGCG 1 cut(s) 173
BstH2I RGCGCY 2 cut(s) 399, 774
BstHHI GCGC 3 cut(s) 175, 398, 773
BstKTI GATC 2 cut(s) 475, 1105
BstMAI GTCTC 1 cut(s) 32
BstMBI GATC 2 cut(s) 472, 1102
BstMWI GCNNNNNNNGC 6 cut(s) 388, 393, 554, 643, 731, 782
BstPAI GACNNNNGTC 1 cut(s) 464
BstSCI CCNGG 3 cut(s) 507, 536, 537
BstSFI CTRYAG 1 cut(s) 635
BstUI CGCG 1 cut(s) 173
BstV2I GAAGAC 1 cut(s) 890
Bsu36I CCTNAGG 1 cut(s) 711
BsuRI GGCC 3 cut(s) 94, 504, 536
BtgI CCRYGG 2 cut(s) 316, 861
Cac8I GCNNGC 5 cut(s) 38, 96, 389, 546, 817
CfoI GCGC 3 cut(s) 175, 398, 773
Cfr13I GGNCC 5 cut(s) 93, 351, 502, 535, 1071
Cfr9I CCCGGG 1 cut(s) 537
CseI GACGC 2 cut(s) 64, 127
Csp6I GTAC 5 cut(s) 45, 421, 433, 993, 1067
CviAII CATG 3 cut(s) 563, 1036, 1097
CviQI GTAC 5 cut(s) 45, 421, 433, 993, 1067
DdeI CTNAG 3 cut(s) 23, 711, 1041
DinI GGCGCC 1 cut(s) 397
DpnI GATC 2 cut(s) 474, 1104
DpnII GATC 2 cut(s) 472, 1102
DraIII CACNNNGTG 1 cut(s) 457
Eam1104I CTCTTC 3 cut(s) 410, 831, 1138
EarI CTCTTC 3 cut(s) 410, 831, 1138
EciI GGCGGA 3 cut(s) 52, 572, 582
Ecl136II GAGCTC 2 cut(s) 544, 1141
Eco24I GRGCYC 2 cut(s) 546, 1143
Eco47I GGWCC 2 cut(s) 351, 1071
Eco47III AGCGCT 1 cut(s) 772
Eco53kI GAGCTC 2 cut(s) 544, 1141
Eco57I CTGAAG 2 cut(s) 434, 1093
Eco81I CCTNAGG 1 cut(s) 711
Eco88I CYCGRG 1 cut(s) 537
EcoICRI GAGCTC 2 cut(s) 544, 1141
EcoO109I RGGNCCY 1 cut(s) 351
EcoRI GAATTC 1 cut(s) 18
EcoT38I GRGCYC 2 cut(s) 546, 1143
EgeI GGCGCC 1 cut(s) 397
EheI GGCGCC 1 cut(s) 397
FaeI CATG 3 cut(s) 566, 1039, 1100
FaiI YATR 8 cut(s) 113, 564, 615, 988, 1037, 1098, 1127, 1129
FaqI GGGAC 1 cut(s) 364
FatI CATG 3 cut(s) 562, 1035, 1096
FauI CCCGC 1 cut(s) 103
FriOI GRGCYC 2 cut(s) 546, 1143
FspBI CTAG 1 cut(s) 786
GlaI GCGC 3 cut(s) 174, 397, 772
GsaI CCCAGC 1 cut(s) 386
GsuI CTGGAG 1 cut(s) 424
HaeII RGCGCY 2 cut(s) 399, 774
HaeIII GGCC 3 cut(s) 94, 504, 536
HapII CCGG 3 cut(s) 11, 508, 538
HgaI GACGC 2 cut(s) 64, 127
HhaI GCGC 3 cut(s) 175, 398, 773
Hin1I GRCGYC 3 cut(s) 56, 138, 396
Hin1II CATG 3 cut(s) 566, 1039, 1100
Hin6I GCGC 3 cut(s) 173, 396, 771
HinP1I GCGC 3 cut(s) 173, 396, 771
HincII GTYRAC 1 cut(s) 930
HindII GTYRAC 1 cut(s) 930
HindIII AAGCTT 1 cut(s) 817
HinfI GANTC 4 cut(s) 304, 683, 800, 1028
HpaI GTTAAC 1 cut(s) 930
HpaII CCGG 3 cut(s) 11, 508, 538
HphI GGTGA 1 cut(s) 267
Hpy166II GTNNAC 1 cut(s) 930
Hpy188I TCNGA 9 cut(s) 204, 332, 414, 477, 529, 805, 856, 955, 1138
Hpy188III TCNNGA 8 cut(s) 349, 441, 626, 687, 911, 965, 1111, 1153
Hpy8I GTNNAC 1 cut(s) 930
Hpy99I CGWCG 6 cut(s) 58, 93, 143, 290, 293, 470
HpyAV CCTTC 5 cut(s) 220, 472, 928, 1048, 1084
HpyCH4III ACNGT 6 cut(s) 320, 962, 992, 1071, 1148, 1174
HpyCH4IV ACGT 3 cut(s) 291, 423, 593
HpyCH4V TGCA 4 cut(s) 637, 776, 812, 832
HpyF10VI GCNNNNNNNGC 6 cut(s) 388, 393, 554, 643, 731, 782
HpyF3I CTNAG 3 cut(s) 23, 711, 1041
HpySE526I ACGT 3 cut(s) 291, 423, 593
Hsp92I GRCGYC 3 cut(s) 56, 138, 396
Hsp92II CATG 3 cut(s) 566, 1039, 1100
HspAI GCGC 3 cut(s) 173, 396, 771
KasI GGCGCC 1 cut(s) 395
KflI GGGWCCC 1 cut(s) 351
KspAI GTTAAC 1 cut(s) 930
Kzo9I GATC 2 cut(s) 472, 1102
LmnI GCTCC 5 cut(s) 13, 541, 559, 689, 1138
LweI GCATC 2 cut(s) 418, 799
MaeI CTAG 1 cut(s) 786
MaeII ACGT 3 cut(s) 291, 423, 593
MaeIII GTNAC 1 cut(s) 457
MalI GATC 2 cut(s) 474, 1104
MboI GATC 2 cut(s) 472, 1102
MboII GAAGA 6 cut(s) 427, 673, 818, 848, 890, 1125
MfeI CAATTG 1 cut(s) 1023
MhlI GDGCHC 3 cut(s) 489, 546, 1143
MluCI AATT 8 cut(s) 18, 100, 630, 791, 1023, 1062, 1078, 1114
Mly113I GGCGCC 1 cut(s) 396
MlyI GAGTC 2 cut(s) 809, 1037
MmeI TCCRAC 2 cut(s) 310, 783
MroXI GAANNNNTTC 1 cut(s) 209
MseI TTAA 8 cut(s) 666, 705, 764, 794, 929, 1166, 1176, 1186
MspA1I CMGCKG 2 cut(s) 640, 695
MspI CCGG 3 cut(s) 11, 508, 538
MspR9I CCNGG 3 cut(s) 509, 538, 539
MunI CAATTG 1 cut(s) 1023
Mva1269I GAATGC 1 cut(s) 447
MvnI CGCG 1 cut(s) 173
MwoI GCNNNNNNNGC 6 cut(s) 388, 393, 554, 643, 731, 782
NarI GGCGCC 1 cut(s) 396
NciI CCSGG 3 cut(s) 509, 538, 539
NdeII GATC 2 cut(s) 472, 1102
NlaIII CATG 3 cut(s) 566, 1039, 1100
NlaIV GGNNCC 4 cut(s) 352, 353, 397, 619
NmeAIII GCCGAG 1 cut(s) 372
NmuCI GTSAC 1 cut(s) 457
PcsI WCGNNNNNNNCGW 1 cut(s) 185
PctI GAATGC 1 cut(s) 447
PdmI GAANNNNTTC 1 cut(s) 209
PfeI GAWTC 2 cut(s) 304, 683
PflFI GACNNNGTC 1 cut(s) 58
PleI GAGTC 2 cut(s) 808, 1036
PluTI GGCGCC 1 cut(s) 399
PpsI GAGTC 2 cut(s) 808, 1036
Ppu21I YACGTR 1 cut(s) 424
PpuMI RGGWCCY 1 cut(s) 351
PshAI GACNNNNGTC 1 cut(s) 464
Psp124BI GAGCTC 2 cut(s) 546, 1143
Psp5II RGGWCCY 1 cut(s) 351
PspFI CCCAGC 1 cut(s) 382
PspN4I GGNNCC 4 cut(s) 352, 353, 397, 619
PspPI GGNCC 5 cut(s) 93, 351, 502, 535, 1071
PspPPI RGGWCCY 1 cut(s) 351
PstI CTGCAG 1 cut(s) 639
PsyI GACNNNGTC 1 cut(s) 58
PvuII CAGCTG 1 cut(s) 695
RsaI GTAC 5 cut(s) 46, 422, 434, 994, 1068
RsaNI GTAC 5 cut(s) 45, 421, 433, 993, 1067
SacI GAGCTC 2 cut(s) 546, 1143
SaqAI TTAA 8 cut(s) 666, 705, 764, 794, 929, 1166, 1176, 1186
Sau3AI GATC 2 cut(s) 472, 1102
Sau96I GGNCC 5 cut(s) 93, 351, 502, 535, 1071
ScaI AGTACT 1 cut(s) 994
SchI GAGTC 2 cut(s) 809, 1037
ScrFI CCNGG 3 cut(s) 509, 538, 539
SduI GDGCHC 3 cut(s) 489, 546, 1143
SfaNI GCATC 2 cut(s) 418, 799
SfcI CTRYAG 1 cut(s) 635
SfoI GGCGCC 1 cut(s) 397
SinI GGWCC 2 cut(s) 351, 1071
SmaI CCCGGG 1 cut(s) 539
Sse9I AATT 8 cut(s) 18, 100, 630, 791, 1023, 1062, 1078, 1114
SsiI CCGC 6 cut(s) 63, 96, 505, 557, 567, 640
SspDI GGCGCC 1 cut(s) 395
SspMI CTAG 1 cut(s) 786
SstI GAGCTC 2 cut(s) 546, 1143
StyD4I CCNGG 3 cut(s) 507, 536, 537
TaaI ACNGT 6 cut(s) 320, 962, 992, 1071, 1148, 1174
TaiI ACGT 3 cut(s) 294, 426, 596
TaqI TCGA 2 cut(s) 130, 1143
TasI AATT 8 cut(s) 18, 100, 630, 791, 1023, 1062, 1078, 1114
TatI WGTACW 1 cut(s) 992
TauI GCSGC 1 cut(s) 507
TfiI GAWTC 2 cut(s) 304, 683
Tru1I TTAA 8 cut(s) 666, 705, 764, 794, 929, 1166, 1176, 1186
Tru9I TTAA 8 cut(s) 666, 705, 764, 794, 929, 1166, 1176, 1186
TseFI GTSAC 1 cut(s) 457
Tsp45I GTSAC 1 cut(s) 457
TspDTI ATGAA 1 cut(s) 1024
TspGWI ACGGA 1 cut(s) 77
TspMI CCCGGG 1 cut(s) 537
Tth111I GACNNNGTC 1 cut(s) 58
VpaK11BI GGWCC 2 cut(s) 351, 1071
XapI RAATTY 4 cut(s) 18, 100, 630, 1114
XmaI CCCGGG 1 cut(s) 537
XmnI GAANNNNTTC 1 cut(s) 209
XspI CTAG 1 cut(s) 786
ZrmI AGTACT 1 cut(s) 994
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.