FvH4_1g16150

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0010933
Physical Location & Seq
Forward (+)
9241794 .. 9243281
1488 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g16150.t1

Sequence Viewer

Length: 1056 bp
ATGGAGTGGATTCGAGGTGAGCAGCTCGGCCGTGGAAGCTTTGCCACCGTCAACTCAGCAAAACCTAAAAACCAGTCTTGTAAAACCCCACCATTGATGGCCGTCAAGTCTTCTGAAGCCTCCTTCTCTGCTCTGCTCAAGAACGAGAAGCAAGTCCTCAACCAAATCGGCTGCTGCCCACAAATCATCCGATGCTTTGGAGACGATCACACCTTCGAAAACGGCATGGCGTTCTACAACTTGTTCTTGGAGTACGCCAGCGGTGGCTGCTTGGCCGATCAGCTCAAGAAAAACGGCGGCCGGTTGCCGGAGATAGATGTTAAACGATATGCTAGATCAGTTCTTAAAGGCCTTCGCTTTGTTCATTCAAAAGGGTTTGTTCACTGCGACGTAAAGCTTGCGAATGTTCTTGTTTTTGAGAATGGCGCGGCGAAGATTGCCGATTTCGGACTTGCCAAAAAGGCGGGACCAACCGGAAACAGAGTTGAAGTCCGGGGAACTCCTCTGTACATGTCGCCGGAATCAGTGAACGAGCAAGAGTTCGAGTCGCCAGCGGATATTTGGGCTTTTGGGTGTCTAGTGGCGGAGATGGTGACCGGGAAACCGGTCTGGAATCACGGGCAGGGCTCCAATATGTGCAAGCTTTTGATGAGAATAGGAGGAGATGAATCGCCGGAAATTCCAGAGGAGTTGAGTGAAGAAGGTAAAGATTTTCTCGGAAAGTGTTTTGTGAAGGATCAGAGGAATAGATGGACGGCGGAGATGCTTTTGGAACATCCCTTTGTTATTTCTGATGACACTGTTCCATTGGAGGAGGATGTTGAAGACGAGTTGTACTCATCTCCAAGAGGTCCTTTTGATTTCCCTGATTGGGTTTCAATGATAAGCTCAGAAATTTCACCTGAATTTTCTGAGTTGGCGGCAAGTGATTTGGATTCCAGCTTTGATTGGTCGTCGGAAACGAGTTCAAACCCGGCGGCCGTTCGGTTGCGGCGTCTGGTGACGGATGAGACACAGGATTGGTCAGTTTCCCGGAGTTGGGTCACAGTCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

352

Amino Acids

39.06

Weight (kDa)

4.97

Isoelectric Point (pI)

47.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 5 - 262 2.1e-56 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 6 - 257 5e-35 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010933)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50310 AT4G36950 AT5G67080
fragaria_vesca FvH4_1g16150
malus_domestica MD02G1173600.v1.1 MD15G1285100.v1.1
prunus_persica Prupe.2G016400_v2.0.a1 Prupe.3G259700_v2.0.a1 Prupe.7G132100_v2.0.a1
pyrus_communis pycom02g14000
rosa_chinensis RchiOBHm_Chr2g0105831
rosa_laevigata RLG00000017463
rosa_multiflora Rmu_sc0004386.1_g000026
rosa_roxburghii Rroxscaffold_2G00137860
rosa_rugosa Rorug02G0130700
rosa_samantha Rh2AG181800 Rh2BG191600 Rh2CG186200 Rh2DG188200
rosa_wichuraiana Rw2G014250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 428
AclWI GGATC 1 cut(s) 744
AcoI YGGCCR 5 cut(s) 28, 99, 273, 298, 978
AcsI RAATTY 3 cut(s) 678, 894, 905
AcuI CTGAAG 1 cut(s) 135
AcyI GRCGYC 1 cut(s) 994
AfaI GTAC 3 cut(s) 254, 509, 836
AfiI CCNNNNNNNGG 4 cut(s) 307, 871, 1038, 1039
AflIII ACRYGT 1 cut(s) 510
AgeI ACCGGT 1 cut(s) 604
AgsI TTSAA 5 cut(s) 369, 488, 824, 879, 969
AluBI AGCT 7 cut(s) 25, 39, 283, 397, 643, 888, 942
AluI AGCT 7 cut(s) 25, 39, 283, 397, 643, 888, 942
Alw26I GTCTC 2 cut(s) 195, 1004
AlwI GGATC 1 cut(s) 744
AoxI GGCC 6 cut(s) 28, 99, 273, 298, 349, 978
ApeKI GCWGC 4 cut(s) 22, 171, 174, 267
ApoI RAATTY 3 cut(s) 678, 894, 905
AsiGI ACCGGT 1 cut(s) 604
AspLEI GCGC 1 cut(s) 428
AspS9I GGNCC 2 cut(s) 467, 851
AsuC2I CCSGG 4 cut(s) 494, 598, 974, 1033
AsuHPI GGTGA 4 cut(s) 29, 604, 891, 1012
AsuII TTCGAA 1 cut(s) 216
AvaII GGWCC 2 cut(s) 467, 851
BanII GRGCYC 1 cut(s) 629
BbsI GAAGAC 2 cut(s) 102, 831
BbvI GCAGC 4 cut(s) 34, 158, 161, 254
BccI CCATC 3 cut(s) 91, 583, 744
BceAI ACGGC 6 cut(s) 15, 86, 238, 310, 771, 965
BcnI CCSGG 4 cut(s) 494, 598, 974, 1033
BcoDI GTCTC 2 cut(s) 195, 1004
BfaI CTAG 2 cut(s) 333, 578
BglI GCCNNNNNGGC 1 cut(s) 461
BisI GCNGC 9 cut(s) 23, 172, 175, 268, 298, 429, 921, 978, 992
BlsI GCNGC 9 cut(s) 24, 173, 176, 269, 299, 430, 922, 979, 993
Bme1390I CCNGG 4 cut(s) 494, 598, 974, 1033
Bme18I GGWCC 2 cut(s) 467, 851
BmgT120I GGNCC 2 cut(s) 467, 851
BmiI GGNNCC 2 cut(s) 468, 628
BmrFI CCNGG 4 cut(s) 494, 598, 974, 1033
BmsI GCATC 2 cut(s) 182, 753
BpiI GAAGAC 2 cut(s) 102, 831
BplI GAGNNNNNCTC 2 cut(s) 821, 853
Bpu14I TTCGAA 1 cut(s) 216
BpuEI CTTGAG 2 cut(s) 122, 269
BpuMI CCSGG 4 cut(s) 494, 598, 974, 1033
BsaHI GRCGYC 1 cut(s) 994
BsaJI CCNNGG 2 cut(s) 31, 493
BsaWI WCCGGW 2 cut(s) 473, 604
Bsc4I CCNNNNNNNGG 4 cut(s) 307, 871, 1038, 1039
Bse118I RCCGGY 2 cut(s) 300, 604
Bse1I ACTGG 1 cut(s) 73
BseDI CCNNGG 2 cut(s) 31, 493
BseGI GGATG 4 cut(s) 186, 775, 823, 1012
BseLI CCNNNNNNNGG 4 cut(s) 307, 871, 1038, 1039
BseMII CTCAG 3 cut(s) 69, 903, 903
BseNI ACTGG 1 cut(s) 73
BseRI GAGGAG 4 cut(s) 492, 675, 701, 827
BseX3I CGGCCG 3 cut(s) 28, 298, 978
BseXI GCAGC 4 cut(s) 34, 158, 161, 254
Bsh1236I CGCG 1 cut(s) 428
Bsh1285I CGRYCG 3 cut(s) 31, 301, 981
BshFI GGCC 6 cut(s) 30, 101, 275, 300, 351, 980
BshTI ACCGGT 1 cut(s) 604
BsiEI CGRYCG 3 cut(s) 31, 301, 981
BslFI GGGAC 1 cut(s) 480
BslI CCNNNNNNNGG 4 cut(s) 307, 871, 1038, 1039
BsmAI GTCTC 2 cut(s) 195, 1004
BsmBI CGTCTC 1 cut(s) 195
BsmFI GGGAC 1 cut(s) 480
BsnI GGCC 6 cut(s) 30, 101, 275, 300, 351, 980
Bsp119I TTCGAA 1 cut(s) 216
Bsp1286I GDGCHC 1 cut(s) 629
Bsp1407I TGTACA 1 cut(s) 507
Bsp143I GATC 4 cut(s) 205, 277, 335, 736
BspANI GGCC 6 cut(s) 30, 101, 275, 300, 351, 980
BspCNI CTCAG 3 cut(s) 68, 902, 904
BspFNI CGCG 1 cut(s) 428
BspLI GGNNCC 2 cut(s) 468, 628
BspPI GGATC 1 cut(s) 744
BspT104I TTCGAA 1 cut(s) 216
BsrFI RCCGGY 2 cut(s) 300, 604
BsrGI TGTACA 1 cut(s) 507
BsrI ACTGG 1 cut(s) 73
BssAI RCCGGY 2 cut(s) 300, 604
BssECI CCNNGG 2 cut(s) 31, 493
BssMI GATC 4 cut(s) 205, 277, 335, 736
BssNI GRCGYC 1 cut(s) 994
Bst4CI ACNGT 3 cut(s) 49, 802, 1048
BstACI GRCGYC 1 cut(s) 994
BstAUI TGTACA 1 cut(s) 507
BstBI TTCGAA 1 cut(s) 216
BstC8I GCNNGC 4 cut(s) 259, 399, 552, 641
BstDEI CTNAG 3 cut(s) 55, 889, 912
BstDSI CCRYGG 1 cut(s) 31
BstEII GGTNACC 1 cut(s) 592
BstF5I GGATG 4 cut(s) 186, 775, 823, 1012
BstFNI CGCG 1 cut(s) 428
BstHHI GCGC 1 cut(s) 428
BstKTI GATC 4 cut(s) 208, 280, 338, 739
BstMAI GTCTC 2 cut(s) 195, 1004
BstMBI GATC 4 cut(s) 205, 277, 335, 736
BstMCI CGRYCG 3 cut(s) 31, 301, 981
BstMWI GCNNNNNNNGC 4 cut(s) 36, 267, 437, 461
BstNSI RCATGY 1 cut(s) 514
BstPI GGTNACC 1 cut(s) 592
BstSCI CCNGG 4 cut(s) 492, 596, 972, 1031
BstUI CGCG 1 cut(s) 428
BstV1I GCAGC 4 cut(s) 34, 158, 161, 254
BstV2I GAAGAC 2 cut(s) 102, 831
BstZI CGGCCG 3 cut(s) 28, 298, 978
BsuRI GGCC 6 cut(s) 30, 101, 275, 300, 351, 980
BtgI CCRYGG 1 cut(s) 31
BtsCI GGATG 4 cut(s) 186, 775, 823, 1012
BtsI GCAGTG 1 cut(s) 382
BtsIMutI CAGTG 3 cut(s) 382, 531, 798
Cac8I GCNNGC 4 cut(s) 259, 399, 552, 641
CfoI GCGC 1 cut(s) 428
Cfr10I RCCGGY 2 cut(s) 300, 604
Cfr13I GGNCC 2 cut(s) 467, 851
CseI GACGC 1 cut(s) 983
Csp6I GTAC 3 cut(s) 253, 508, 835
CspAI ACCGGT 1 cut(s) 604
CviAII CATG 2 cut(s) 226, 511
CviQI GTAC 3 cut(s) 253, 508, 835
DdeI CTNAG 3 cut(s) 55, 889, 912
DpnI GATC 4 cut(s) 207, 279, 337, 738
DpnII GATC 4 cut(s) 205, 277, 335, 736
EaeI YGGCCR 5 cut(s) 28, 99, 273, 298, 978
EagI CGGCCG 3 cut(s) 28, 298, 978
EciI GGCGGA 2 cut(s) 599, 773
EclXI CGGCCG 3 cut(s) 28, 298, 978
Eco147I AGGCCT 1 cut(s) 351
Eco24I GRGCYC 1 cut(s) 629
Eco47I GGWCC 2 cut(s) 467, 851
Eco52I CGGCCG 3 cut(s) 28, 298, 978
Eco57I CTGAAG 1 cut(s) 135
Eco91I GGTNACC 1 cut(s) 592
EcoO109I RGGNCCY 1 cut(s) 851
EcoO65I GGTNACC 1 cut(s) 592
EcoT38I GRGCYC 1 cut(s) 629
Esp3I CGTCTC 1 cut(s) 195
FaeI CATG 2 cut(s) 229, 514
FaiI YATR 4 cut(s) 227, 330, 512, 635
FalI AAGNNNNNCTT 2 cut(s) 838, 870
FaqI GGGAC 1 cut(s) 480
FatI CATG 2 cut(s) 225, 510
FauI CCCGC 1 cut(s) 457
Fnu4HI GCNGC 9 cut(s) 23, 172, 175, 268, 298, 429, 921, 978, 992
FokI GGATG 4 cut(s) 173, 762, 830, 1019
FriOI GRGCYC 1 cut(s) 629
Fsp4HI GCNGC 9 cut(s) 23, 172, 175, 268, 298, 429, 921, 978, 992
FspBI CTAG 2 cut(s) 333, 578
GlaI GCGC 1 cut(s) 427
GluI GCNGC 9 cut(s) 23, 172, 175, 268, 298, 429, 921, 978, 992
HaeIII GGCC 6 cut(s) 30, 101, 275, 300, 351, 980
HgaI GACGC 1 cut(s) 983
HhaI GCGC 1 cut(s) 428
Hin1I GRCGYC 1 cut(s) 994
Hin1II CATG 2 cut(s) 229, 514
Hin6I GCGC 1 cut(s) 426
HinP1I GCGC 1 cut(s) 426
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HindIII AAGCTT 3 cut(s) 37, 395, 641
HinfI GANTC 6 cut(s) 10, 521, 545, 613, 668, 935
HphI GGTGA 4 cut(s) 29, 604, 891, 1012
Hpy166II GTNNAC 3 cut(s) 52, 382, 529
Hpy188I TCNGA 9 cut(s) 115, 191, 449, 719, 741, 793, 892, 913, 958
Hpy188III TCNNGA 4 cut(s) 139, 286, 610, 683
Hpy8I GTNNAC 3 cut(s) 52, 382, 529
Hpy99I CGWCG 2 cut(s) 392, 958
HpyAV CCTTC 5 cut(s) 133, 223, 362, 695, 727
HpyCH4III ACNGT 3 cut(s) 49, 802, 1048
HpyCH4IV ACGT 1 cut(s) 390
HpyCH4V TGCA 1 cut(s) 639
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 267, 437, 461
HpyF3I CTNAG 3 cut(s) 55, 889, 912
HpySE526I ACGT 1 cut(s) 390
Hsp92I GRCGYC 1 cut(s) 994
Hsp92II CATG 2 cut(s) 229, 514
HspAI GCGC 1 cut(s) 426
Kzo9I GATC 4 cut(s) 205, 277, 335, 736
LmnI GCTCC 1 cut(s) 632
Lsp1109I GCAGC 4 cut(s) 34, 158, 161, 254
LweI GCATC 2 cut(s) 182, 753
MaeI CTAG 2 cut(s) 333, 578
MaeII ACGT 1 cut(s) 390
MaeIII GTNAC 3 cut(s) 592, 1000, 1042
MalI GATC 4 cut(s) 207, 279, 337, 738
MboI GATC 4 cut(s) 205, 277, 335, 736
MboII GAAGA 4 cut(s) 102, 445, 710, 836
MhlI GDGCHC 1 cut(s) 629
MluCI AATT 3 cut(s) 678, 894, 905
MlyI GAGTC 1 cut(s) 554
MmeI TCCRAC 1 cut(s) 936
MseI TTAA 2 cut(s) 321, 345
MspA1I CMGCKG 2 cut(s) 261, 554
MspR9I CCNGG 4 cut(s) 494, 598, 974, 1033
MvnI CGCG 1 cut(s) 428
MwoI GCNNNNNNNGC 4 cut(s) 36, 267, 437, 461
NciI CCSGG 4 cut(s) 494, 598, 974, 1033
NdeII GATC 4 cut(s) 205, 277, 335, 736
NlaIII CATG 2 cut(s) 229, 514
NlaIV GGNNCC 2 cut(s) 468, 628
NmeAIII GCCGAG 1 cut(s) 6
NmuCI GTSAC 3 cut(s) 592, 1000, 1042
NspI RCATGY 1 cut(s) 514
NspV TTCGAA 1 cut(s) 216
PceI AGGCCT 1 cut(s) 351
PciI ACATGT 1 cut(s) 510
PcsI WCGNNNNNNNCGW 2 cut(s) 959, 991
PfeI GAWTC 5 cut(s) 10, 521, 613, 668, 935
PfoI TCCNGGA 1 cut(s) 1031
PinAI ACCGGT 1 cut(s) 604
PkrI GCNGC 9 cut(s) 24, 173, 176, 269, 299, 430, 922, 979, 993
PleI GAGTC 1 cut(s) 553
PpsI GAGTC 1 cut(s) 553
PpuMI RGGWCCY 1 cut(s) 851
PscI ACATGT 1 cut(s) 510
Psp5II RGGWCCY 1 cut(s) 851
PspEI GGTNACC 1 cut(s) 592
PspN4I GGNNCC 2 cut(s) 468, 628
PspPI GGNCC 2 cut(s) 467, 851
PspPPI RGGWCCY 1 cut(s) 851
RsaI GTAC 3 cut(s) 254, 509, 836
RsaNI GTAC 3 cut(s) 253, 508, 835
SaqAI TTAA 2 cut(s) 321, 345
SatI GCNGC 9 cut(s) 23, 172, 175, 268, 298, 429, 921, 978, 992
Sau3AI GATC 4 cut(s) 205, 277, 335, 736
Sau96I GGNCC 2 cut(s) 467, 851
SchI GAGTC 1 cut(s) 554
ScrFI CCNGG 4 cut(s) 494, 598, 974, 1033
SduI GDGCHC 1 cut(s) 629
SfaNI GCATC 2 cut(s) 182, 753
SfuI TTCGAA 1 cut(s) 216
SinI GGWCC 2 cut(s) 467, 851
SmlI CTYRAG 2 cut(s) 137, 284
SmoI CTYRAG 2 cut(s) 137, 284
Sse9I AATT 3 cut(s) 678, 894, 905
SseBI AGGCCT 1 cut(s) 351
SspMI CTAG 2 cut(s) 333, 578
StuI AGGCCT 1 cut(s) 351
StyD4I CCNGG 4 cut(s) 492, 596, 972, 1031
TaaI ACNGT 3 cut(s) 49, 802, 1048
TaiI ACGT 1 cut(s) 393
TaqI TCGA 3 cut(s) 13, 216, 543
TasI AATT 3 cut(s) 678, 894, 905
TatI WGTACW 2 cut(s) 507, 834
TauI GCSGC 5 cut(s) 300, 431, 923, 980, 994
TfiI GAWTC 5 cut(s) 10, 521, 613, 668, 935
Tru1I TTAA 2 cut(s) 321, 345
Tru9I TTAA 2 cut(s) 321, 345
TscAI CASTG 3 cut(s) 389, 531, 805
TseFI GTSAC 3 cut(s) 592, 1000, 1042
TseI GCWGC 4 cut(s) 22, 171, 174, 267
Tsp45I GTSAC 3 cut(s) 592, 1000, 1042
TspDTI ATGAA 2 cut(s) 353, 681
TspGWI ACGGA 1 cut(s) 1019
TspRI CASTG 3 cut(s) 389, 531, 805
VpaK11BI GGWCC 2 cut(s) 467, 851
XapI RAATTY 3 cut(s) 678, 894, 905
XceI RCATGY 1 cut(s) 514
XcmI CCANNNNNNNNNTGG 1 cut(s) 558
XspI CTAG 2 cut(s) 333, 578
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.