Rh2CG186200

Serine/Threonine protein kinases, catalytic domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0010933
Physical Location & Seq
Forward (+)
17336650 .. 17338035
1386 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG186200.1

Sequence Viewer

Length: 1053 bp
ATGGAGTGGATTCGAGGCGAGCAACTTGGCCGTGGAAGCTTTGCTACCGTCAGTTCAGCAAAACCCAAAAATCAGTCTACTAAAACCCCATCATTGATGGCCGTCAAGTCTTCTGAAGCTTCCTTCTCTTCTCTGCTCAAGAACGAGAAACAAGTTCTCGACCAAATCGGCTCCTGCCCGCAAGTCGTCCGCTGCTTCGGAGATGATCACACCTTCGAAAACGGCACGGCGTTCTACAACTTGTTCTTGGAGTACGCCAGCGGCGGCTGCTTGGCCGATCAGCTCAAGAAAAACGGCGGCCGGTTAGCGGAGATAGATGTTAAACGATATGCCAGAGCAGTTCTTAAAGGCCTTCGCTTTGTTCATTCAAAAGGGTTTGTTCACTGCGACGTAAAGTTAGCGAATGTTCTTGTTTTTGAGAATGGTGCGGCGAAGATTGCCGATTTCGGACTTGCCAAAAAGGCAGGGCCGACCGGAAACAGAGTCGAAGTCCGGGGAACTCCGCTGTACATGTCGCCGGAATCGGTGAACGAGCAAGAGTTCGAGTCGCCGGCGGATATTTGGGCTTTTGGGTGTTTAGTGGCGGAGATGGTGACCGGGAAACCGGTCTGGAATGATGGGCCGGGCTCGAATATGTGCAAGCTTTTGATGAGAATAGGGGGAGATGAGTCGCCGGAAATTCCAGAAGAGTTGAGTGAAGAAGGGAAAGATTTTCTTGGAAAGTGTTTTGTGAAGGATCAGAGAAAGAGATGGACGGCTGAGATGCTCTTGGAACACCCCTTTGTTATTTCTGATGATACTGTTCCATTGGAGGACGTTGAAGACGAGTTGTTCTCATCTCCAAGAGGTCCTTTTGATTTCCCTGATTGGGTTTCGATGACTAGTTCGGAAATTTCACCAGAATTTTCTGAGTTGGTGACAAGTGATTTGAATTCCAGCTTTGATTGGTCGTCGGAAACGAGTTCTAATCCTGCGGCCGGTCGTTTGCGGCGGCTGGTGACGGATGAGACACATGATTGGTCGGTTTCCGGAAGTTGGGTGACTATCAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

350

Amino Acids

38.66

Weight (kDa)

4.96

Isoelectric Point (pI)

43.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 5 - 262 3.1e-56 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 6 - 257 8e-37 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010933)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G50310 AT4G36950 AT5G67080
fragaria_vesca FvH4_1g16150
malus_domestica MD02G1173600.v1.1 MD15G1285100.v1.1
prunus_persica Prupe.2G016400_v2.0.a1 Prupe.3G259700_v2.0.a1 Prupe.7G132100_v2.0.a1
pyrus_communis pycom02g14000
rosa_chinensis RchiOBHm_Chr2g0105831
rosa_laevigata RLG00000017463
rosa_multiflora Rmu_sc0004386.1_g000026
rosa_roxburghii Rroxscaffold_2G00137860
rosa_rugosa Rorug02G0130700
rosa_samantha Rh2AG181800 Rh2BG191600 Rh2CG186200 Rh2DG188200
rosa_wichuraiana Rw2G014250

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 77
AccIII TCCGGA 1 cut(s) 1028
AclWI GGATC 1 cut(s) 744
AcoI YGGCCR 5 cut(s) 28, 99, 273, 298, 975
AcsI RAATTY 4 cut(s) 678, 891, 902, 931
AcuI CTGAAG 1 cut(s) 135
AfaI GTAC 2 cut(s) 254, 509
AfiI CCNNNNNNNGG 4 cut(s) 307, 868, 977, 1035
AflIII ACRYGT 1 cut(s) 510
AgeI ACCGGT 1 cut(s) 604
AgsI TTSAA 3 cut(s) 369, 821, 931
AhlI ACTAGT 1 cut(s) 881
AluBI AGCT 5 cut(s) 39, 119, 283, 643, 939
AluI AGCT 5 cut(s) 39, 119, 283, 643, 939
Alw26I GTCTC 1 cut(s) 1001
AlwI GGATC 1 cut(s) 744
Aor13HI TCCGGA 1 cut(s) 1028
AoxI GGCC 8 cut(s) 28, 99, 273, 298, 349, 467, 620, 975
ApeKI GCWGC 2 cut(s) 192, 267
ApoI RAATTY 4 cut(s) 678, 891, 902, 931
ArsI GACNNNNNNTTYG 2 cut(s) 910, 942
AsiGI ACCGGT 1 cut(s) 604
AspS9I GGNCC 3 cut(s) 467, 620, 848
AsuC2I CCSGG 3 cut(s) 494, 598, 624
AsuHPI GGTGA 6 cut(s) 538, 604, 888, 928, 1009, 1051
AsuII TTCGAA 1 cut(s) 216
AvaII GGWCC 1 cut(s) 848
BanII GRGCYC 1 cut(s) 629
BarI GAAGNNNNNNTAC 2 cut(s) 28, 60
BbsI GAAGAC 2 cut(s) 102, 828
BbvI GCAGC 2 cut(s) 179, 254
BccI CCATC 5 cut(s) 91, 97, 583, 611, 744
BceAI ACGGC 6 cut(s) 15, 86, 238, 243, 310, 771
BclI TGATCA 1 cut(s) 205
BcnI CCSGG 3 cut(s) 494, 598, 624
BcoDI GTCTC 1 cut(s) 1001
BcuI ACTAGT 1 cut(s) 881
BfaI CTAG 1 cut(s) 882
BglI GCCNNNNNGGC 1 cut(s) 461
BisI GCNGC 9 cut(s) 193, 262, 265, 268, 298, 429, 975, 989, 992
BlsI GCNGC 9 cut(s) 194, 263, 266, 269, 299, 430, 976, 990, 993
Bme1390I CCNGG 3 cut(s) 494, 598, 624
Bme18I GGWCC 1 cut(s) 848
BmgT120I GGNCC 3 cut(s) 467, 620, 848
BmiI GGNNCC 1 cut(s) 172
BmrFI CCNGG 3 cut(s) 494, 598, 624
BmsI GCATC 1 cut(s) 753
BpiI GAAGAC 2 cut(s) 102, 828
BplI GAGNNNNNCTC 2 cut(s) 818, 850
Bpu14I TTCGAA 1 cut(s) 216
BpuEI CTTGAG 2 cut(s) 122, 269
BpuMI CCSGG 3 cut(s) 494, 598, 624
BsaJI CCNNGG 2 cut(s) 31, 493
BsaWI WCCGGW 3 cut(s) 473, 604, 1028
Bsc4I CCNNNNNNNGG 4 cut(s) 307, 868, 977, 1035
Bse118I RCCGGY 4 cut(s) 300, 550, 604, 977
BseAI TCCGGA 1 cut(s) 1028
BseDI CCNNGG 2 cut(s) 31, 493
BseGI GGATG 1 cut(s) 1009
BseLI CCNNNNNNNGG 4 cut(s) 307, 868, 977, 1035
BseMII CTCAG 2 cut(s) 750, 900
BseX3I CGGCCG 2 cut(s) 298, 975
BseXI GCAGC 2 cut(s) 179, 254
Bsh1285I CGRYCG 4 cut(s) 301, 474, 978, 982
BshFI GGCC 8 cut(s) 30, 101, 275, 300, 351, 469, 622, 977
BshTI ACCGGT 1 cut(s) 604
BsiEI CGRYCG 4 cut(s) 301, 474, 978, 982
BslI CCNNNNNNNGG 4 cut(s) 307, 868, 977, 1035
BsmAI GTCTC 1 cut(s) 1001
BsnI GGCC 8 cut(s) 30, 101, 275, 300, 351, 469, 622, 977
Bsp119I TTCGAA 1 cut(s) 216
Bsp1286I GDGCHC 1 cut(s) 629
Bsp13I TCCGGA 1 cut(s) 1028
Bsp1407I TGTACA 1 cut(s) 507
Bsp143I GATC 3 cut(s) 205, 277, 736
BspANI GGCC 8 cut(s) 30, 101, 275, 300, 351, 469, 622, 977
BspCNI CTCAG 2 cut(s) 751, 901
BspEI TCCGGA 1 cut(s) 1028
BspLI GGNNCC 1 cut(s) 172
BspPI GGATC 1 cut(s) 744
BspT104I TTCGAA 1 cut(s) 216
BsrFI RCCGGY 4 cut(s) 300, 550, 604, 977
BsrGI TGTACA 1 cut(s) 507
BssAI RCCGGY 4 cut(s) 300, 550, 604, 977
BssECI CCNNGG 2 cut(s) 31, 493
BssMI GATC 3 cut(s) 205, 277, 736
Bst4CI ACNGT 2 cut(s) 49, 802
Bst6I CTCTTC 2 cut(s) 133, 681
BstAUI TGTACA 1 cut(s) 507
BstBI TTCGAA 1 cut(s) 216
BstC8I GCNNGC 5 cut(s) 20, 179, 259, 552, 641
BstDEI CTNAG 2 cut(s) 759, 909
BstDSI CCRYGG 1 cut(s) 31
BstEII GGTNACC 1 cut(s) 592
BstF5I GGATG 1 cut(s) 1009
BstKTI GATC 3 cut(s) 208, 280, 739
BstMAI GTCTC 1 cut(s) 1001
BstMBI GATC 3 cut(s) 205, 277, 736
BstMCI CGRYCG 4 cut(s) 301, 474, 978, 982
BstMWI GCNNNNNNNGC 4 cut(s) 36, 267, 437, 461
BstNSI RCATGY 1 cut(s) 514
BstPI GGTNACC 1 cut(s) 592
BstSCI CCNGG 3 cut(s) 492, 596, 622
BstV1I GCAGC 2 cut(s) 179, 254
BstV2I GAAGAC 2 cut(s) 102, 828
BstZI CGGCCG 2 cut(s) 298, 975
BsuRI GGCC 8 cut(s) 30, 101, 275, 300, 351, 469, 622, 977
BtgI CCRYGG 1 cut(s) 31
BtsCI GGATG 1 cut(s) 1009
BtsI GCAGTG 1 cut(s) 382
BtsIMutI CAGTG 1 cut(s) 382
Cac8I GCNNGC 5 cut(s) 20, 179, 259, 552, 641
Cfr10I RCCGGY 4 cut(s) 300, 550, 604, 977
Cfr13I GGNCC 3 cut(s) 467, 620, 848
Csp6I GTAC 2 cut(s) 253, 508
CspAI ACCGGT 1 cut(s) 604
CviAII CATG 2 cut(s) 511, 1013
CviQI GTAC 2 cut(s) 253, 508
DdeI CTNAG 2 cut(s) 759, 909
DpnI GATC 3 cut(s) 207, 279, 738
DpnII GATC 3 cut(s) 205, 277, 736
EaeI YGGCCR 5 cut(s) 28, 99, 273, 298, 975
EagI CGGCCG 2 cut(s) 298, 975
Eam1104I CTCTTC 2 cut(s) 133, 681
EarI CTCTTC 2 cut(s) 133, 681
EciI GGCGGA 2 cut(s) 569, 599
EclXI CGGCCG 2 cut(s) 298, 975
Eco147I AGGCCT 1 cut(s) 351
Eco24I GRGCYC 1 cut(s) 629
Eco47I GGWCC 1 cut(s) 848
Eco52I CGGCCG 2 cut(s) 298, 975
Eco57I CTGAAG 1 cut(s) 135
Eco91I GGTNACC 1 cut(s) 592
EcoO109I RGGNCCY 1 cut(s) 848
EcoO65I GGTNACC 1 cut(s) 592
EcoRI GAATTC 1 cut(s) 931
EcoT38I GRGCYC 1 cut(s) 629
FaeI CATG 2 cut(s) 514, 1016
FaiI YATR 4 cut(s) 330, 512, 635, 1014
FalI AAGNNNNNCTT 4 cut(s) 699, 731, 835, 867
FatI CATG 2 cut(s) 510, 1012
FauI CCCGC 1 cut(s) 186
FbaI TGATCA 1 cut(s) 205
FblI GTMKAC 1 cut(s) 77
Fnu4HI GCNGC 9 cut(s) 193, 262, 265, 268, 298, 429, 975, 989, 992
FokI GGATG 1 cut(s) 1016
FriOI GRGCYC 1 cut(s) 629
Fsp4HI GCNGC 9 cut(s) 193, 262, 265, 268, 298, 429, 975, 989, 992
FspBI CTAG 1 cut(s) 882
GluI GCNGC 9 cut(s) 193, 262, 265, 268, 298, 429, 975, 989, 992
HaeIII GGCC 8 cut(s) 30, 101, 275, 300, 351, 469, 622, 977
Hin1II CATG 2 cut(s) 514, 1016
HindIII AAGCTT 3 cut(s) 37, 117, 641
HinfI GANTC 5 cut(s) 10, 483, 521, 545, 668
HphI GGTGA 6 cut(s) 538, 604, 888, 928, 1009, 1051
Hpy166II GTNNAC 3 cut(s) 78, 382, 529
Hpy188I TCNGA 8 cut(s) 115, 200, 449, 741, 793, 889, 910, 955
Hpy188III TCNNGA 6 cut(s) 139, 158, 286, 610, 683, 1029
Hpy8I GTNNAC 3 cut(s) 78, 382, 529
Hpy99I CGWCG 2 cut(s) 392, 955
HpyAV CCTTC 5 cut(s) 133, 223, 362, 695, 727
HpyCH4III ACNGT 2 cut(s) 49, 802
HpyCH4IV ACGT 2 cut(s) 390, 816
HpyCH4V TGCA 1 cut(s) 639
HpyF10VI GCNNNNNNNGC 4 cut(s) 36, 267, 437, 461
HpyF3I CTNAG 2 cut(s) 759, 909
HpySE526I ACGT 2 cut(s) 390, 816
Hsp92II CATG 2 cut(s) 514, 1016
Kpn2I TCCGGA 1 cut(s) 1028
KroI GCCGGC 1 cut(s) 550
KroNI GCCGGC 1 cut(s) 552
Ksp22I TGATCA 1 cut(s) 205
Kzo9I GATC 3 cut(s) 205, 277, 736
LmnI GCTCC 1 cut(s) 176
Lsp1109I GCAGC 2 cut(s) 179, 254
LweI GCATC 1 cut(s) 753
MaeI CTAG 1 cut(s) 882
MaeII ACGT 2 cut(s) 390, 816
MaeIII GTNAC 4 cut(s) 592, 916, 997, 1039
MalI GATC 3 cut(s) 207, 279, 738
MboI GATC 3 cut(s) 205, 277, 736
MboII GAAGA 6 cut(s) 102, 120, 445, 698, 710, 833
MhlI GDGCHC 1 cut(s) 629
MluCI AATT 4 cut(s) 678, 891, 902, 931
MlyI GAGTC 3 cut(s) 492, 554, 677
MmeI TCCRAC 1 cut(s) 933
MnlI CCTC 3 cut(s) 8, 805, 839
MreI CGCCGGCG 1 cut(s) 550
MroI TCCGGA 1 cut(s) 1028
MroNI GCCGGC 1 cut(s) 550
MseI TTAA 2 cut(s) 321, 345
MspA1I CMGCKG 3 cut(s) 192, 261, 505
MspR9I CCNGG 3 cut(s) 494, 598, 624
MwoI GCNNNNNNNGC 4 cut(s) 36, 267, 437, 461
NaeI GCCGGC 1 cut(s) 552
NciI CCSGG 3 cut(s) 494, 598, 624
NdeII GATC 3 cut(s) 205, 277, 736
NgoMIV GCCGGC 1 cut(s) 550
NlaIII CATG 2 cut(s) 514, 1016
NlaIV GGNNCC 1 cut(s) 172
NmuCI GTSAC 4 cut(s) 592, 916, 997, 1039
NspI RCATGY 1 cut(s) 514
NspV TTCGAA 1 cut(s) 216
PceI AGGCCT 1 cut(s) 351
PciI ACATGT 1 cut(s) 510
PcsI WCGNNNNNNNCGW 2 cut(s) 822, 956
PdiI GCCGGC 1 cut(s) 552
PfeI GAWTC 2 cut(s) 10, 521
PinAI ACCGGT 1 cut(s) 604
PkrI GCNGC 9 cut(s) 194, 263, 266, 269, 299, 430, 976, 990, 993
PleI GAGTC 3 cut(s) 491, 553, 676
PpsI GAGTC 3 cut(s) 491, 553, 676
PpuMI RGGWCCY 1 cut(s) 848
PscI ACATGT 1 cut(s) 510
Psp5II RGGWCCY 1 cut(s) 848
PspEI GGTNACC 1 cut(s) 592
PspN4I GGNNCC 1 cut(s) 172
PspPI GGNCC 3 cut(s) 467, 620, 848
PspPPI RGGWCCY 1 cut(s) 848
RsaI GTAC 2 cut(s) 254, 509
RsaNI GTAC 2 cut(s) 253, 508
SaqAI TTAA 2 cut(s) 321, 345
SatI GCNGC 9 cut(s) 193, 262, 265, 268, 298, 429, 975, 989, 992
Sau3AI GATC 3 cut(s) 205, 277, 736
Sau96I GGNCC 3 cut(s) 467, 620, 848
SchI GAGTC 3 cut(s) 492, 554, 677
ScrFI CCNGG 3 cut(s) 494, 598, 624
SduI GDGCHC 1 cut(s) 629
SfaNI GCATC 1 cut(s) 753
SfuI TTCGAA 1 cut(s) 216
SgrAI CRCCGGYG 1 cut(s) 550
SinI GGWCC 1 cut(s) 848
SmlI CTYRAG 2 cut(s) 137, 284
SmoI CTYRAG 2 cut(s) 137, 284
SpeI ACTAGT 1 cut(s) 881
Sse9I AATT 4 cut(s) 678, 891, 902, 931
SseBI AGGCCT 1 cut(s) 351
SspMI CTAG 1 cut(s) 882
StuI AGGCCT 1 cut(s) 351
StyD4I CCNGG 3 cut(s) 492, 596, 622
TaaI ACNGT 2 cut(s) 49, 802
TaiI ACGT 2 cut(s) 393, 819
TaqI TCGA 7 cut(s) 13, 159, 216, 486, 543, 629, 875
TasI AATT 4 cut(s) 678, 891, 902, 931
TatI WGTACW 1 cut(s) 507
TauI GCSGC 7 cut(s) 264, 267, 300, 431, 977, 991, 994
TfiI GAWTC 2 cut(s) 10, 521
Tru1I TTAA 2 cut(s) 321, 345
Tru9I TTAA 2 cut(s) 321, 345
TscAI CASTG 1 cut(s) 389
TseFI GTSAC 4 cut(s) 592, 916, 997, 1039
TseI GCWGC 2 cut(s) 192, 267
Tsp45I GTSAC 4 cut(s) 592, 916, 997, 1039
TspDTI ATGAA 1 cut(s) 353
TspGWI ACGGA 1 cut(s) 1016
TspRI CASTG 1 cut(s) 389
VpaK11BI GGWCC 1 cut(s) 848
XapI RAATTY 4 cut(s) 678, 891, 902, 931
XceI RCATGY 1 cut(s) 514
XmiI GTMKAC 1 cut(s) 77
XspI CTAG 1 cut(s) 882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.