FvH4_1g18560

Prokaryotic E2 family B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0014640
Physical Location & Seq
Reverse (-)
10906113 .. 10909078
2966 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g18560.t2

Sequence Viewer

Length: 435 bp
ATGGGTTCGTCGTCTGCTCAGCTCCGCCTCATGTCCGATCTCAAAACCATCATTAACGAGCCCCCGGAGGGTTGCAGCGCGAGTCCTATGTCCGATGATAACCTGTTCGTGTGGAGCGCGACTATATTTGGACCTGATGAGACTCCATGGGAAGGTGGTGTGTTCAGTTTGCGGTTGACATTTAGTGAGCGATATCCGGAAAAGCCGCCGCGTGTCCGATTCACGTCCGAGATGTTCCATCCGAATGTTTACCATGATGGCGCACTGTGCATGGATATAATTCAGGATGCATGGTCACCCTGCCACAACTCACTTCTCACTGATCCAAATCCTTCAAGTCCTGCAAACCCTGAGGCTGCTCAACTGTATCAACATGACATAAAAGCTTACAACAAGAGGGTACGCCGCTGTGCTCGCAATTCCATCGAATCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

145

Amino Acids

16.19

Weight (kDa)

5.28

Isoelectric Point (pI)

62.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 9 - 146 9.1e-51 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014640)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18560 FvH4_1g18560 FvH4_1g18560
malus_domestica MD02G1193300.v1.1 MD15G1303600.v1.1
prunus_persica Prupe.6G213500_v2.0.a1
pyrus_communis pycom02g15700 pycom15g26650
rosa_chinensis RchiOBHm_Chr2g0109261
rosa_laevigata RLG00000017729
rosa_multiflora Rmu_sc0001807.1_g000008
rosa_roxburghii Rroxscaffold_2G00134350
rosa_rugosa Rorug02G0158900
rosa_samantha Rh2AG210300 Rh2BG220700 Rh2CG212700 Rh2DG216500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 80, 119, 211
AccIII TCCGGA 1 cut(s) 196
AciI CCGC 5 cut(s) 25, 172, 206, 209, 406
AclWI GGATC 1 cut(s) 317
AfaI GTAC 1 cut(s) 402
AfiI CCNNNNNNNGG 2 cut(s) 68, 152
AgsI TTSAA 1 cut(s) 336
AjiI CACGTC 1 cut(s) 225
AluBI AGCT 2 cut(s) 22, 386
AluI AGCT 2 cut(s) 22, 386
Alw21I GWGCWC 1 cut(s) 415
Alw26I GTCTC 1 cut(s) 134
AlwI GGATC 1 cut(s) 317
Aor13HI TCCGGA 1 cut(s) 196
ApeKI GCWGC 2 cut(s) 75, 356
AspLEI GCGC 3 cut(s) 80, 119, 263
AspS9I GGNCC 1 cut(s) 131
AsuC2I CCSGG 1 cut(s) 65
AsuHPI GGTGA 1 cut(s) 288
AvaII GGWCC 1 cut(s) 131
AxyI CCTNAGG 1 cut(s) 351
BanII GRGCYC 1 cut(s) 63
Bbv12I GWGCWC 1 cut(s) 415
BbvI GCAGC 2 cut(s) 87, 343
BccI CCATC 4 cut(s) 56, 246, 251, 431
BcgI CGANNNNNNTGC 1 cut(s) 406
BcnI CCSGG 1 cut(s) 65
BcoDI GTCTC 1 cut(s) 134
BisI GCNGC 5 cut(s) 76, 206, 209, 357, 406
BlpI GCTNAGC 1 cut(s) 18
BlsI GCNGC 5 cut(s) 77, 207, 210, 358, 407
Bme1390I CCNGG 1 cut(s) 65
Bme18I GGWCC 1 cut(s) 131
BmgBI CACGTC 1 cut(s) 225
BmgT120I GGNCC 1 cut(s) 131
BmrFI CCNGG 1 cut(s) 65
BmsI GCATC 1 cut(s) 277
Bpu1102I GCTNAGC 1 cut(s) 18
BpuMI CCSGG 1 cut(s) 65
BsaBI GATNNNNATC 1 cut(s) 327
BsaJI CCNNGG 2 cut(s) 63, 146
BsaWI WCCGGW 1 cut(s) 196
Bsc4I CCNNNNNNNGG 2 cut(s) 68, 152
Bse21I CCTNAGG 1 cut(s) 351
Bse8I GATNNNNATC 1 cut(s) 327
BseAI TCCGGA 1 cut(s) 196
BseDI CCNNGG 2 cut(s) 63, 146
BseGI GGATG 2 cut(s) 238, 292
BseJI GATNNNNATC 1 cut(s) 327
BseLI CCNNNNNNNGG 2 cut(s) 68, 152
BseMII CTCAG 2 cut(s) 32, 342
BseXI GCAGC 2 cut(s) 87, 343
Bsh1236I CGCG 3 cut(s) 80, 119, 211
BsiHKAI GWGCWC 1 cut(s) 415
BsiSI CCGG 2 cut(s) 65, 197
BslI CCNNNNNNNGG 2 cut(s) 68, 152
BsmAI GTCTC 1 cut(s) 134
Bsp1286I GDGCHC 2 cut(s) 63, 415
Bsp13I TCCGGA 1 cut(s) 196
Bsp143I GATC 2 cut(s) 37, 322
Bsp1720I GCTNAGC 1 cut(s) 18
Bsp19I CCATGG 1 cut(s) 146
BspACI CCGC 5 cut(s) 25, 172, 206, 209, 406
BspCNI CTCAG 2 cut(s) 31, 343
BspEI TCCGGA 1 cut(s) 196
BspFNI CGCG 3 cut(s) 80, 119, 211
BspPI GGATC 1 cut(s) 317
BssECI CCNNGG 2 cut(s) 63, 146
BssMI GATC 2 cut(s) 37, 322
BssT1I CCWWGG 1 cut(s) 146
Bst4CI ACNGT 2 cut(s) 267, 366
BstC8I GCNNGC 1 cut(s) 415
BstDEI CTNAG 3 cut(s) 18, 351, 432
BstDSI CCRYGG 1 cut(s) 146
BstEII GGTNACC 1 cut(s) 294
BstF5I GGATG 2 cut(s) 238, 292
BstFNI CGCG 3 cut(s) 80, 119, 211
BstHHI GCGC 3 cut(s) 80, 119, 263
BstKTI GATC 2 cut(s) 40, 325
BstMAI GTCTC 1 cut(s) 134
BstMBI GATC 2 cut(s) 37, 322
BstMWI GCNNNNNNNGC 2 cut(s) 267, 414
BstPI GGTNACC 1 cut(s) 294
BstSCI CCNGG 1 cut(s) 63
BstUI CGCG 3 cut(s) 80, 119, 211
BstV1I GCAGC 2 cut(s) 87, 343
Bsu36I CCTNAGG 1 cut(s) 351
BtgI CCRYGG 1 cut(s) 146
BtrI CACGTC 1 cut(s) 225
BtsCI GGATG 2 cut(s) 238, 292
BtsIMutI CAGTG 2 cut(s) 263, 318
Cac8I GCNNGC 1 cut(s) 415
CfoI GCGC 3 cut(s) 80, 119, 263
Cfr13I GGNCC 1 cut(s) 131
Csp6I GTAC 1 cut(s) 401
CviAII CATG 6 cut(s) 31, 147, 254, 271, 291, 374
CviJI RGCY 5 cut(s) 22, 61, 205, 356, 386
CviKI_1 RGCY 5 cut(s) 22, 61, 205, 356, 386
CviQI GTAC 1 cut(s) 401
DdeI CTNAG 3 cut(s) 18, 351, 432
DpnI GATC 2 cut(s) 39, 324
DpnII GATC 2 cut(s) 37, 322
EciI GGCGGA 1 cut(s) 14
Eco130I CCWWGG 1 cut(s) 146
Eco24I GRGCYC 1 cut(s) 63
Eco32I GATATC 1 cut(s) 194
Eco47I GGWCC 1 cut(s) 131
Eco81I CCTNAGG 1 cut(s) 351
Eco91I GGTNACC 1 cut(s) 294
EcoO65I GGTNACC 1 cut(s) 294
EcoRV GATATC 1 cut(s) 194
EcoT14I CCWWGG 1 cut(s) 146
EcoT22I ATGCAT 1 cut(s) 292
EcoT38I GRGCYC 1 cut(s) 63
ErhI CCWWGG 1 cut(s) 146
FaeI CATG 6 cut(s) 34, 150, 257, 274, 294, 377
FatI CATG 6 cut(s) 30, 146, 253, 270, 290, 373
Fnu4HI GCNGC 5 cut(s) 76, 206, 209, 357, 406
FokI GGATG 2 cut(s) 225, 299
FriOI GRGCYC 1 cut(s) 63
Fsp4HI GCNGC 5 cut(s) 76, 206, 209, 357, 406
GlaI GCGC 3 cut(s) 79, 118, 262
GluI GCNGC 5 cut(s) 76, 206, 209, 357, 406
HapII CCGG 2 cut(s) 65, 197
HhaI GCGC 3 cut(s) 80, 119, 263
Hin1II CATG 6 cut(s) 34, 150, 257, 274, 294, 377
Hin6I GCGC 3 cut(s) 78, 117, 261
HinP1I GCGC 3 cut(s) 78, 117, 261
HincII GTYRAC 1 cut(s) 177
HindII GTYRAC 1 cut(s) 177
HindIII AAGCTT 1 cut(s) 384
HinfI GANTC 4 cut(s) 82, 142, 219, 428
HpaII CCGG 2 cut(s) 65, 197
HphI GGTGA 1 cut(s) 288
Hpy166II GTNNAC 2 cut(s) 177, 250
Hpy188I TCNGA 5 cut(s) 37, 94, 218, 229, 243
Hpy188III TCNNGA 2 cut(s) 197, 284
Hpy8I GTNNAC 2 cut(s) 177, 250
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 2 cut(s) 146, 342
HpyCH4III ACNGT 2 cut(s) 267, 366
HpyCH4IV ACGT 1 cut(s) 224
HpyCH4V TGCA 4 cut(s) 75, 270, 290, 344
HpyF10VI GCNNNNNNNGC 2 cut(s) 267, 414
HpyF3I CTNAG 3 cut(s) 18, 351, 432
HpySE526I ACGT 1 cut(s) 224
Hsp92II CATG 6 cut(s) 34, 150, 257, 274, 294, 377
HspAI GCGC 3 cut(s) 78, 117, 261
Kpn2I TCCGGA 1 cut(s) 196
Kzo9I GATC 2 cut(s) 37, 322
LmnI GCTCC 2 cut(s) 27, 114
LpnPI CCDG 8 cut(s) 78, 116, 147, 210, 269, 313, 354, 363
Lsp1109I GCAGC 2 cut(s) 87, 343
LweI GCATC 1 cut(s) 277
MaeII ACGT 1 cut(s) 224
MaeIII GTNAC 1 cut(s) 294
MalI GATC 2 cut(s) 39, 324
MboI GATC 2 cut(s) 37, 322
MhlI GDGCHC 2 cut(s) 63, 415
MluCI AATT 2 cut(s) 279, 418
MlyI GAGTC 2 cut(s) 91, 136
MnlI CCTC 4 cut(s) 38, 61, 346, 390
Mph1103I ATGCAT 1 cut(s) 292
MroI TCCGGA 1 cut(s) 196
MseI TTAA 1 cut(s) 54
MslI CAYNNNNRTG 1 cut(s) 243
MspA1I CMGCKG 1 cut(s) 408
MspI CCGG 2 cut(s) 65, 197
MspR9I CCNGG 1 cut(s) 65
MvnI CGCG 3 cut(s) 80, 119, 211
MwoI GCNNNNNNNGC 2 cut(s) 267, 414
NciI CCSGG 1 cut(s) 65
NcoI CCATGG 1 cut(s) 146
NdeII GATC 2 cut(s) 37, 322
NlaIII CATG 6 cut(s) 34, 150, 257, 274, 294, 377
NmuCI GTSAC 1 cut(s) 294
NsiI ATGCAT 1 cut(s) 292
PfeI GAWTC 2 cut(s) 219, 428
PkrI GCNGC 5 cut(s) 77, 207, 210, 358, 407
PleI GAGTC 2 cut(s) 90, 136
PpsI GAGTC 2 cut(s) 90, 136
PspEI GGTNACC 1 cut(s) 294
PspPI GGNCC 1 cut(s) 131
RsaI GTAC 1 cut(s) 402
RsaNI GTAC 1 cut(s) 401
RseI CAYNNNNRTG 1 cut(s) 243
SaqAI TTAA 1 cut(s) 54
SatI GCNGC 5 cut(s) 76, 206, 209, 357, 406
Sau3AI GATC 2 cut(s) 37, 322
Sau96I GGNCC 1 cut(s) 131
SchI GAGTC 2 cut(s) 91, 136
ScrFI CCNGG 1 cut(s) 65
SduI GDGCHC 2 cut(s) 63, 415
SetI ASST 6 cut(s) 24, 105, 136, 157, 227, 388
SfaNI GCATC 1 cut(s) 277
SinI GGWCC 1 cut(s) 131
SmiMI CAYNNNNRTG 1 cut(s) 243
Sse9I AATT 2 cut(s) 279, 418
SsiI CCGC 5 cut(s) 25, 172, 206, 209, 406
StyD4I CCNGG 1 cut(s) 63
StyI CCWWGG 1 cut(s) 146
TaaI ACNGT 2 cut(s) 267, 366
TaiI ACGT 1 cut(s) 227
TaqI TCGA 1 cut(s) 426
TasI AATT 2 cut(s) 279, 418
TauI GCSGC 3 cut(s) 208, 211, 408
TfiI GAWTC 2 cut(s) 219, 428
Tru1I TTAA 1 cut(s) 54
Tru9I TTAA 1 cut(s) 54
TscAI CASTG 2 cut(s) 270, 325
TseFI GTSAC 1 cut(s) 294
TseI GCWGC 2 cut(s) 75, 356
Tsp45I GTSAC 1 cut(s) 294
TspRI CASTG 2 cut(s) 270, 325
VpaK11BI GGWCC 1 cut(s) 131
Zsp2I ATGCAT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.