MD15G1303600.v1.1

Prokaryotic E2 family B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014640
Physical Location & Seq
Reverse (-)
29506171 .. 29508907
2737 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1303600.v1.1.491

Sequence Viewer

Length: 480 bp
ATGGCGTCGTCGGCACAGCTCCGGCTCATGTCCGATCTCAGAACCATCATCAATGAGCCTCCCGAGGGTTGCAGCGCCAGCCCTCTGTCCGACGATAACCTGATGGTTTGGAGCGCCACCATATTTGGGCCCGACGAGACTCCATGGGAAGGTTGTGGTGTTTTTAGTTTGAGGTTGACATTTAGTGAACGTTATCCCGAAAAGCCACCTCGGGTTCGATTCACCTCCGAAATCTTCCATCCAAATGTTTATCATGACGGTACACTGTGCATGGATATAATTCAGGATGCATGGTCACCATGCCACAATGTATCTACAATTTTGACATCTGTTCAATCACTTCTCACCGATCCAAACGCTGCAAGTCCAGCAAACCCTGATGCTGCTCACATGTATCAGCATGACATACAAGCTTACAACAAGTCAGAGGGTACGCCGTTCTGCTCGTCATTCCATCGAGTCTTTGTAGTAGCTGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

160

Amino Acids

17.65

Weight (kDa)

4.89

Isoelectric Point (pI)

51.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 8 - 141 6.9e-46 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014640)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18560 FvH4_1g18560 FvH4_1g18560
malus_domestica MD02G1193300.v1.1 MD15G1303600.v1.1
prunus_persica Prupe.6G213500_v2.0.a1
pyrus_communis pycom02g15700 pycom15g26650
rosa_chinensis RchiOBHm_Chr2g0109261
rosa_laevigata RLG00000017729
rosa_multiflora Rmu_sc0001807.1_g000008
rosa_roxburghii Rroxscaffold_2G00134350
rosa_rugosa Rorug02G0158900
rosa_samantha Rh2AG210300 Rh2BG220700 Rh2CG212700 Rh2DG216500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclI AACGTT 1 cut(s) 190
AclWI GGATC 1 cut(s) 344
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 262, 433
AfiI CCNNNNNNNGG 3 cut(s) 65, 126, 149
AflIII ACRYGT 1 cut(s) 390
AgsI TTSAA 1 cut(s) 335
AluBI AGCT 3 cut(s) 19, 413, 473
AluI AGCT 3 cut(s) 19, 413, 473
Alw26I GTCTC 1 cut(s) 131
AlwI GGATC 1 cut(s) 344
Ama87I CYCGRG 2 cut(s) 62, 210
AoxI GGCC 1 cut(s) 128
ApaI GGGCCC 1 cut(s) 132
ApeKI GCWGC 3 cut(s) 72, 359, 383
AspLEI GCGC 2 cut(s) 77, 116
AspS9I GGNCC 2 cut(s) 128, 129
AsuHPI GGTGA 3 cut(s) 214, 288, 337
AvaI CYCGRG 2 cut(s) 62, 210
BaeGI GKGCMC 1 cut(s) 132
BanII GRGCYC 1 cut(s) 132
BbvI GCAGC 3 cut(s) 84, 346, 370
BccI CCATC 4 cut(s) 53, 97, 246, 462
BceAI ACGGC 1 cut(s) 421
BcoDI GTCTC 1 cut(s) 131
BfoI RGCGCY 2 cut(s) 78, 117
BisI GCNGC 3 cut(s) 73, 360, 384
BlsI GCNGC 3 cut(s) 74, 361, 385
BmeT110I CYCGRG 2 cut(s) 62, 210
BmgT120I GGNCC 2 cut(s) 128, 129
BmiI GGNNCC 1 cut(s) 130
BmsI GCATC 2 cut(s) 277, 370
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 63, 143, 209
Bsc4I CCNNNNNNNGG 3 cut(s) 65, 126, 149
BseDI CCNNGG 3 cut(s) 63, 143, 209
BseGI GGATG 2 cut(s) 238, 292
BseLI CCNNNNNNNGG 3 cut(s) 65, 126, 149
BseMII CTCAG 1 cut(s) 52
BseSI GKGCMC 1 cut(s) 132
BseXI GCAGC 3 cut(s) 84, 346, 370
BshFI GGCC 1 cut(s) 130
BsiHKCI CYCGRG 2 cut(s) 62, 210
BsiSI CCGG 1 cut(s) 22
BslI CCNNNNNNNGG 3 cut(s) 65, 126, 149
BsmAI GTCTC 1 cut(s) 131
BsnI GGCC 1 cut(s) 130
BsoBI CYCGRG 2 cut(s) 62, 210
Bsp120I GGGCCC 1 cut(s) 128
Bsp1286I GDGCHC 1 cut(s) 132
Bsp143I GATC 2 cut(s) 34, 349
Bsp19I CCATGG 1 cut(s) 143
BspANI GGCC 1 cut(s) 130
BspCNI CTCAG 1 cut(s) 51
BspHI TCATGA 1 cut(s) 253
BspLI GGNNCC 1 cut(s) 130
BspPI GGATC 1 cut(s) 344
BssECI CCNNGG 3 cut(s) 63, 143, 209
BssMI GATC 2 cut(s) 34, 349
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 143
Bst4CI ACNGT 2 cut(s) 260, 267
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 1 cut(s) 79
BstDEI CTNAG 1 cut(s) 38
BstDSI CCRYGG 1 cut(s) 143
BstEII GGTNACC 1 cut(s) 294
BstF5I GGATG 2 cut(s) 238, 292
BstH2I RGCGCY 2 cut(s) 78, 117
BstHHI GCGC 2 cut(s) 77, 116
BstKTI GATC 2 cut(s) 37, 352
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 2 cut(s) 34, 349
BstMWI GCNNNNNNNGC 3 cut(s) 11, 78, 368
BstNSI RCATGY 1 cut(s) 394
BstPI GGTNACC 1 cut(s) 294
BstSLI GKGCMC 1 cut(s) 132
BstV1I GCAGC 3 cut(s) 84, 346, 370
BsuRI GGCC 1 cut(s) 130
BtgI CCRYGG 1 cut(s) 143
BtsCI GGATG 2 cut(s) 238, 292
BtsIMutI CAGTG 1 cut(s) 263
Cac8I GCNNGC 1 cut(s) 79
CciI TCATGA 1 cut(s) 253
CfoI GCGC 2 cut(s) 77, 116
Cfr13I GGNCC 2 cut(s) 128, 129
Csp6I GTAC 2 cut(s) 261, 432
CspCI CAANNNNNGTGG 2 cut(s) 106, 141
CviAII CATG 8 cut(s) 28, 144, 254, 271, 291, 300, 391, 401
CviJI RGCY 8 cut(s) 19, 25, 58, 81, 130, 205, 413, 473
CviKI_1 RGCY 8 cut(s) 19, 25, 58, 81, 130, 205, 413, 473
CviQI GTAC 2 cut(s) 261, 432
DdeI CTNAG 1 cut(s) 38
DpnI GATC 2 cut(s) 36, 351
DpnII GATC 2 cut(s) 34, 349
Eco130I CCWWGG 1 cut(s) 143
Eco24I GRGCYC 1 cut(s) 132
Eco88I CYCGRG 2 cut(s) 62, 210
Eco91I GGTNACC 1 cut(s) 294
EcoO65I GGTNACC 1 cut(s) 294
EcoT14I CCWWGG 1 cut(s) 143
EcoT22I ATGCAT 1 cut(s) 292
EcoT38I GRGCYC 1 cut(s) 132
ErhI CCWWGG 1 cut(s) 143
FaeI CATG 8 cut(s) 31, 147, 257, 274, 294, 303, 394, 404
FatI CATG 8 cut(s) 27, 143, 253, 270, 290, 299, 390, 400
Fnu4HI GCNGC 3 cut(s) 73, 360, 384
FokI GGATG 2 cut(s) 225, 299
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 3 cut(s) 73, 360, 384
GlaI GCGC 2 cut(s) 76, 115
GluI GCNGC 3 cut(s) 73, 360, 384
HaeII RGCGCY 2 cut(s) 78, 117
HaeIII GGCC 1 cut(s) 130
HapII CCGG 1 cut(s) 22
HhaI GCGC 2 cut(s) 77, 116
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 8 cut(s) 31, 147, 257, 274, 294, 303, 394, 404
Hin6I GCGC 2 cut(s) 75, 114
HinP1I GCGC 2 cut(s) 75, 114
HincII GTYRAC 1 cut(s) 177
HindII GTYRAC 1 cut(s) 177
HindIII AAGCTT 1 cut(s) 411
HinfI GANTC 3 cut(s) 139, 219, 459
HpaII CCGG 1 cut(s) 22
HphI GGTGA 3 cut(s) 214, 288, 337
Hpy166II GTNNAC 3 cut(s) 177, 188, 263
Hpy188I TCNGA 5 cut(s) 34, 41, 91, 229, 427
Hpy188III TCNNGA 4 cut(s) 62, 197, 254, 284
Hpy8I GTNNAC 3 cut(s) 177, 188, 263
Hpy99I CGWCG 4 cut(s) 10, 13, 95, 137
HpyAV CCTTC 1 cut(s) 143
HpyCH4III ACNGT 2 cut(s) 260, 267
HpyCH4IV ACGT 1 cut(s) 190
HpyCH4V TGCA 4 cut(s) 72, 270, 290, 362
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 78, 368
HpyF3I CTNAG 1 cut(s) 38
HpySE526I ACGT 1 cut(s) 190
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 8 cut(s) 31, 147, 257, 274, 294, 303, 394, 404
HspAI GCGC 2 cut(s) 75, 114
Kzo9I GATC 2 cut(s) 34, 349
LmnI GCTCC 2 cut(s) 24, 111
LpnPI CCDG 6 cut(s) 35, 91, 113, 269, 381, 390
Lsp1109I GCAGC 3 cut(s) 84, 346, 370
LweI GCATC 2 cut(s) 277, 370
MaeII ACGT 1 cut(s) 190
MaeIII GTNAC 1 cut(s) 294
MalI GATC 2 cut(s) 36, 351
MboI GATC 2 cut(s) 34, 349
MboII GAAGA 1 cut(s) 226
MhlI GDGCHC 1 cut(s) 132
MluCI AATT 2 cut(s) 279, 318
MlyI GAGTC 2 cut(s) 133, 468
MmeI TCCRAC 1 cut(s) 114
MnlI CCTC 7 cut(s) 58, 69, 93, 165, 219, 235, 421
Mph1103I ATGCAT 1 cut(s) 292
MslI CAYNNNNRTG 1 cut(s) 243
MspI CCGG 1 cut(s) 22
MwoI GCNNNNNNNGC 3 cut(s) 11, 78, 368
NcoI CCATGG 1 cut(s) 143
NdeII GATC 2 cut(s) 34, 349
NlaIII CATG 8 cut(s) 31, 147, 257, 274, 294, 303, 394, 404
NlaIV GGNNCC 1 cut(s) 130
NmuCI GTSAC 1 cut(s) 294
NsiI ATGCAT 1 cut(s) 292
NspI RCATGY 1 cut(s) 394
PagI TCATGA 1 cut(s) 253
PciI ACATGT 1 cut(s) 390
PfeI GAWTC 1 cut(s) 219
PkrI GCNGC 3 cut(s) 74, 361, 385
PleI GAGTC 2 cut(s) 133, 467
PpsI GAGTC 2 cut(s) 133, 467
PscI ACATGT 1 cut(s) 390
Psp1406I AACGTT 1 cut(s) 190
PspEI GGTNACC 1 cut(s) 294
PspN4I GGNNCC 1 cut(s) 130
PspOMI GGGCCC 1 cut(s) 128
PspPI GGNCC 2 cut(s) 128, 129
RsaI GTAC 2 cut(s) 262, 433
RsaNI GTAC 2 cut(s) 261, 432
RseI CAYNNNNRTG 1 cut(s) 243
SatI GCNGC 3 cut(s) 73, 360, 384
Sau3AI GATC 2 cut(s) 34, 349
Sau96I GGNCC 2 cut(s) 128, 129
SchI GAGTC 2 cut(s) 133, 468
SduI GDGCHC 1 cut(s) 132
SetI ASST 9 cut(s) 21, 102, 154, 176, 193, 211, 227, 415, 475
SfaNI GCATC 2 cut(s) 277, 370
SmiMI CAYNNNNRTG 1 cut(s) 243
Sse9I AATT 2 cut(s) 279, 318
StyI CCWWGG 1 cut(s) 143
TaaI ACNGT 2 cut(s) 260, 267
TaiI ACGT 1 cut(s) 193
TaqI TCGA 2 cut(s) 217, 457
TasI AATT 2 cut(s) 279, 318
TfiI GAWTC 1 cut(s) 219
TscAI CASTG 1 cut(s) 270
TseFI GTSAC 1 cut(s) 294
TseI GCWGC 3 cut(s) 72, 359, 383
Tsp45I GTSAC 1 cut(s) 294
TspRI CASTG 1 cut(s) 270
XceI RCATGY 1 cut(s) 394
Zsp2I ATGCAT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.