pycom15g26650

ubiquitin-conjugating enzyme

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014640
Physical Location & Seq
Reverse (-)
21675469 .. 21677661
2193 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g26650.1

Sequence Viewer

Length: 477 bp
ATGGCGTCGTCGGCACAGCTCCGGCTCATGTCCGATCTCAGAACCATCATCAACGAGCCTCCCGAGGGTTGCAGCGCCAGCCCTCTGTCCGACGATAACCTGATGGTTTGGAGCGCCACCATATTTGGGCCCGACGAGACTCCATGGGAAGGTTGTCGGGAGATCTGTGGTGTTTTTAGTTTGAGATTGACATTTAGTGAACGTTATCCCGAAAAGCCACCTCGGGTTCGATTCACCTCCGAAATCTTCCATCCAAATGTTTATCGTGACGGTACACTGTGCATGGATATAATTCAGGATGCATGGTCACCATGCCACAATGTATCTACGATTTTGACATCTGTTCAATCACTTCTCACCGATCCAAACGCTGCAAGTCCAGCAAACCCTGATGCGGCTCACATGTATCAGCATGACATACAAGCTTACAACAAGAGGGTACGCCGTTCTGCTCGTCATTCCATCGAGTCTTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

159

Amino Acids

17.77

Weight (kDa)

5.46

Isoelectric Point (pI)

61.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 8 - 149 3.3e-46 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014640)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18560 FvH4_1g18560 FvH4_1g18560
malus_domestica MD02G1193300.v1.1 MD15G1303600.v1.1
prunus_persica Prupe.6G213500_v2.0.a1
pyrus_communis pycom02g15700 pycom15g26650
rosa_chinensis RchiOBHm_Chr2g0109261
rosa_laevigata RLG00000017729
rosa_multiflora Rmu_sc0001807.1_g000008
rosa_roxburghii Rroxscaffold_2G00134350
rosa_rugosa Rorug02G0158900
rosa_samantha Rh2AG210300 Rh2BG220700 Rh2CG212700 Rh2DG216500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 395
AclI AACGTT 1 cut(s) 202
AclWI GGATC 1 cut(s) 356
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 274, 441
AfiI CCNNNNNNNGG 4 cut(s) 65, 126, 149, 394
AflIII ACRYGT 1 cut(s) 402
AgsI TTSAA 1 cut(s) 347
AluBI AGCT 2 cut(s) 19, 425
AluI AGCT 2 cut(s) 19, 425
Alw26I GTCTC 1 cut(s) 131
AlwI GGATC 1 cut(s) 356
Ama87I CYCGRG 2 cut(s) 62, 222
AoxI GGCC 1 cut(s) 128
ApaI GGGCCC 1 cut(s) 132
ApeKI GCWGC 2 cut(s) 72, 371
AspLEI GCGC 2 cut(s) 77, 116
AspS9I GGNCC 2 cut(s) 128, 129
AsuHPI GGTGA 3 cut(s) 226, 300, 349
AvaI CYCGRG 2 cut(s) 62, 222
BaeGI GKGCMC 1 cut(s) 132
BanII GRGCYC 1 cut(s) 132
BbvI GCAGC 2 cut(s) 84, 358
BccI CCATC 4 cut(s) 53, 97, 258, 470
BceAI ACGGC 1 cut(s) 429
BcoDI GTCTC 1 cut(s) 131
BfoI RGCGCY 2 cut(s) 78, 117
BglII AGATCT 1 cut(s) 162
BisI GCNGC 3 cut(s) 73, 372, 396
BlsI GCNGC 3 cut(s) 74, 373, 397
BmeT110I CYCGRG 2 cut(s) 62, 222
BmgT120I GGNCC 2 cut(s) 128, 129
BmiI GGNNCC 1 cut(s) 130
BmsI GCATC 2 cut(s) 289, 382
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 63, 143, 221
Bsc4I CCNNNNNNNGG 4 cut(s) 65, 126, 149, 394
BseDI CCNNGG 3 cut(s) 63, 143, 221
BseGI GGATG 2 cut(s) 250, 304
BseLI CCNNNNNNNGG 4 cut(s) 65, 126, 149, 394
BseMII CTCAG 1 cut(s) 52
BseSI GKGCMC 1 cut(s) 132
BseXI GCAGC 2 cut(s) 84, 358
BshFI GGCC 1 cut(s) 130
BsiHKCI CYCGRG 2 cut(s) 62, 222
BsiSI CCGG 1 cut(s) 22
BslI CCNNNNNNNGG 4 cut(s) 65, 126, 149, 394
BsmAI GTCTC 1 cut(s) 131
BsnI GGCC 1 cut(s) 130
BsoBI CYCGRG 2 cut(s) 62, 222
Bsp120I GGGCCC 1 cut(s) 128
Bsp1286I GDGCHC 1 cut(s) 132
Bsp143I GATC 3 cut(s) 34, 162, 361
Bsp19I CCATGG 1 cut(s) 143
BspACI CCGC 1 cut(s) 395
BspANI GGCC 1 cut(s) 130
BspCNI CTCAG 1 cut(s) 51
BspLI GGNNCC 1 cut(s) 130
BspPI GGATC 1 cut(s) 356
BssECI CCNNGG 3 cut(s) 63, 143, 221
BssMI GATC 3 cut(s) 34, 162, 361
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 143
Bst4CI ACNGT 2 cut(s) 272, 279
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 1 cut(s) 79
BstDEI CTNAG 1 cut(s) 38
BstDSI CCRYGG 1 cut(s) 143
BstEII GGTNACC 1 cut(s) 306
BstF5I GGATG 2 cut(s) 250, 304
BstH2I RGCGCY 2 cut(s) 78, 117
BstHHI GCGC 2 cut(s) 77, 116
BstKTI GATC 3 cut(s) 37, 165, 364
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 3 cut(s) 34, 162, 361
BstMWI GCNNNNNNNGC 3 cut(s) 11, 78, 380
BstNSI RCATGY 1 cut(s) 406
BstPI GGTNACC 1 cut(s) 306
BstSLI GKGCMC 1 cut(s) 132
BstV1I GCAGC 2 cut(s) 84, 358
BstX2I RGATCY 1 cut(s) 162
BstYI RGATCY 1 cut(s) 162
BsuRI GGCC 1 cut(s) 130
BtgI CCRYGG 1 cut(s) 143
BtsCI GGATG 2 cut(s) 250, 304
BtsIMutI CAGTG 1 cut(s) 275
Cac8I GCNNGC 1 cut(s) 79
CfoI GCGC 2 cut(s) 77, 116
Cfr13I GGNCC 2 cut(s) 128, 129
Csp6I GTAC 2 cut(s) 273, 440
CspCI CAANNNNNGTGG 2 cut(s) 106, 141
CviAII CATG 7 cut(s) 28, 144, 283, 303, 312, 403, 413
CviJI RGCY 8 cut(s) 19, 25, 58, 81, 130, 217, 398, 425
CviKI_1 RGCY 8 cut(s) 19, 25, 58, 81, 130, 217, 398, 425
CviQI GTAC 2 cut(s) 273, 440
DdeI CTNAG 1 cut(s) 38
DpnI GATC 3 cut(s) 36, 164, 363
DpnII GATC 3 cut(s) 34, 162, 361
Eco130I CCWWGG 1 cut(s) 143
Eco24I GRGCYC 1 cut(s) 132
Eco88I CYCGRG 2 cut(s) 62, 222
Eco91I GGTNACC 1 cut(s) 306
EcoO65I GGTNACC 1 cut(s) 306
EcoT14I CCWWGG 1 cut(s) 143
EcoT22I ATGCAT 1 cut(s) 304
EcoT38I GRGCYC 1 cut(s) 132
ErhI CCWWGG 1 cut(s) 143
FaeI CATG 7 cut(s) 31, 147, 286, 306, 315, 406, 416
FatI CATG 7 cut(s) 27, 143, 282, 302, 311, 402, 412
Fnu4HI GCNGC 3 cut(s) 73, 372, 396
FokI GGATG 2 cut(s) 237, 311
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 3 cut(s) 73, 372, 396
GlaI GCGC 2 cut(s) 76, 115
GluI GCNGC 3 cut(s) 73, 372, 396
HaeII RGCGCY 2 cut(s) 78, 117
HaeIII GGCC 1 cut(s) 130
HapII CCGG 1 cut(s) 22
HhaI GCGC 2 cut(s) 77, 116
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 7 cut(s) 31, 147, 286, 306, 315, 406, 416
Hin6I GCGC 2 cut(s) 75, 114
HinP1I GCGC 2 cut(s) 75, 114
HindIII AAGCTT 1 cut(s) 423
HinfI GANTC 3 cut(s) 139, 231, 467
HpaII CCGG 1 cut(s) 22
HphI GGTGA 3 cut(s) 226, 300, 349
Hpy166II GTNNAC 2 cut(s) 200, 275
Hpy188I TCNGA 4 cut(s) 34, 41, 91, 241
Hpy188III TCNNGA 5 cut(s) 62, 158, 209, 266, 296
Hpy8I GTNNAC 2 cut(s) 200, 275
Hpy99I CGWCG 4 cut(s) 10, 13, 95, 137
HpyAV CCTTC 1 cut(s) 143
HpyCH4III ACNGT 2 cut(s) 272, 279
HpyCH4IV ACGT 1 cut(s) 202
HpyCH4V TGCA 4 cut(s) 72, 282, 302, 374
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 78, 380
HpyF3I CTNAG 1 cut(s) 38
HpySE526I ACGT 1 cut(s) 202
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 7 cut(s) 31, 147, 286, 306, 315, 406, 416
HspAI GCGC 2 cut(s) 75, 114
Kzo9I GATC 3 cut(s) 34, 162, 361
LmnI GCTCC 2 cut(s) 24, 111
LpnPI CCDG 6 cut(s) 35, 91, 113, 281, 393, 402
Lsp1109I GCAGC 2 cut(s) 84, 358
LweI GCATC 2 cut(s) 289, 382
MaeII ACGT 1 cut(s) 202
MaeIII GTNAC 2 cut(s) 266, 306
MalI GATC 3 cut(s) 36, 164, 363
MboI GATC 3 cut(s) 34, 162, 361
MboII GAAGA 1 cut(s) 238
MflI RGATCY 1 cut(s) 162
MhlI GDGCHC 1 cut(s) 132
MluCI AATT 1 cut(s) 291
MlyI GAGTC 2 cut(s) 133, 476
MmeI TCCRAC 1 cut(s) 114
MnlI CCTC 6 cut(s) 58, 69, 93, 231, 247, 429
Mph1103I ATGCAT 1 cut(s) 304
MslI CAYNNNNRTG 1 cut(s) 255
MspI CCGG 1 cut(s) 22
MwoI GCNNNNNNNGC 3 cut(s) 11, 78, 380
NcoI CCATGG 1 cut(s) 143
NdeII GATC 3 cut(s) 34, 162, 361
NlaIII CATG 7 cut(s) 31, 147, 286, 306, 315, 406, 416
NlaIV GGNNCC 1 cut(s) 130
NmuCI GTSAC 2 cut(s) 266, 306
NsiI ATGCAT 1 cut(s) 304
NspI RCATGY 1 cut(s) 406
PciI ACATGT 1 cut(s) 402
PcsI WCGNNNNNNNCGW 1 cut(s) 60
PfeI GAWTC 1 cut(s) 231
PkrI GCNGC 3 cut(s) 74, 373, 397
PleI GAGTC 2 cut(s) 133, 475
PpsI GAGTC 2 cut(s) 133, 475
PscI ACATGT 1 cut(s) 402
Psp1406I AACGTT 1 cut(s) 202
PspEI GGTNACC 1 cut(s) 306
PspN4I GGNNCC 1 cut(s) 130
PspOMI GGGCCC 1 cut(s) 128
PspPI GGNCC 2 cut(s) 128, 129
PsuI RGATCY 1 cut(s) 162
RsaI GTAC 2 cut(s) 274, 441
RsaNI GTAC 2 cut(s) 273, 440
RseI CAYNNNNRTG 1 cut(s) 255
SatI GCNGC 3 cut(s) 73, 372, 396
Sau3AI GATC 3 cut(s) 34, 162, 361
Sau96I GGNCC 2 cut(s) 128, 129
SchI GAGTC 2 cut(s) 133, 476
SduI GDGCHC 1 cut(s) 132
SetI ASST 7 cut(s) 21, 102, 154, 205, 223, 239, 427
SfaNI GCATC 2 cut(s) 289, 382
SmiMI CAYNNNNRTG 1 cut(s) 255
Sse9I AATT 1 cut(s) 291
SsiI CCGC 1 cut(s) 395
StyI CCWWGG 1 cut(s) 143
TaaI ACNGT 2 cut(s) 272, 279
TaiI ACGT 1 cut(s) 205
TaqI TCGA 2 cut(s) 229, 465
TasI AATT 1 cut(s) 291
TauI GCSGC 1 cut(s) 398
TfiI GAWTC 1 cut(s) 231
TscAI CASTG 1 cut(s) 282
TseFI GTSAC 2 cut(s) 266, 306
TseI GCWGC 2 cut(s) 72, 371
Tsp45I GTSAC 2 cut(s) 266, 306
TspRI CASTG 1 cut(s) 282
XceI RCATGY 1 cut(s) 406
Zsp2I ATGCAT 1 cut(s) 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.