Rh2BG220700

Prokaryotic E2 family B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0014640
Physical Location & Seq
Reverse (-)
21350629 .. 21353644
3016 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG220700.1

Sequence Viewer

Length: 546 bp
ATGGGATCGTCCTCTGCTCAGCTCCGGCTCATGTCCGATCTCAAAACCATCATAAACGAGCCTCCGGAGGGTTGCAGCGCAAGTCCCACGTCCGATGATAACCTGTTCGTGTGGAGCGCGACCATATTTGGGCCCGATGAGACCCCTTGGGAAGGTAGATCTGTTCCATGTTTTGTTCTTTCTTTAACTTTAACTGAAAAGATTTGGTTTTTGATGTGGGATTGGAAATTCAATTCAGGTGGTGTTTTCAGTTTGCGGTTGACCTTCAGTGAGCGCTATCCCGAAAAGCCGCCGCGTGTCCGGTTCACGTCCGAGATGTTCCATCCAAATGTTTACCATGATGGTGCACTGTGCATGGATATAATTCAGGATGCATGGTCACCCTGCCACAACGTATCAACAATTTTAACATCTGTTCAATCACTTCTCACTGATCCAAATCCTTCAAGTCCTGCAAACCCTGAGGCTGCTCAAATGTATCAGCATGACATAAAAGCTTACAACAAGAGGGTACGCCGCTGTGCTCGTGATTCCATCGAGTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

181

Amino Acids

20.49

Weight (kDa)

5.33

Isoelectric Point (pI)

58.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 9 - 64 7.9e-08 Ubiquitin-conjugating enzyme
UQ_con PF00179 79 - 174 4.2e-36 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014640)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18560 FvH4_1g18560 FvH4_1g18560
malus_domestica MD02G1193300.v1.1 MD15G1303600.v1.1
prunus_persica Prupe.6G213500_v2.0.a1
pyrus_communis pycom02g15700 pycom15g26650
rosa_chinensis RchiOBHm_Chr2g0109261
rosa_laevigata RLG00000017729
rosa_multiflora Rmu_sc0001807.1_g000008
rosa_roxburghii Rroxscaffold_2G00134350
rosa_rugosa Rorug02G0158900
rosa_samantha Rh2AG210300 Rh2BG220700 Rh2CG212700 Rh2DG216500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 119, 295
AccIII TCCGGA 1 cut(s) 64
AciI CCGC 4 cut(s) 256, 290, 293, 517
AclWI GGATC 2 cut(s) 13, 428
AcsI RAATTY 1 cut(s) 227
AcuI CTGAAG 1 cut(s) 250
AfaI GTAC 1 cut(s) 513
AfeI AGCGCT 1 cut(s) 275
AfiI CCNNNNNNNGG 3 cut(s) 68, 129, 152
AgsI TTSAA 3 cut(s) 232, 419, 447
AjiI CACGTC 2 cut(s) 90, 309
AluBI AGCT 2 cut(s) 22, 497
AluI AGCT 2 cut(s) 22, 497
Alw21I GWGCWC 2 cut(s) 349, 526
Alw26I GTCTC 1 cut(s) 134
Alw44I GTGCAC 1 cut(s) 345
AlwI GGATC 2 cut(s) 13, 428
Aor13HI TCCGGA 1 cut(s) 64
Aor51HI AGCGCT 1 cut(s) 275
AoxI GGCC 1 cut(s) 131
ApaI GGGCCC 1 cut(s) 135
ApaLI GTGCAC 1 cut(s) 345
ApeKI GCWGC 2 cut(s) 75, 467
ApoI RAATTY 1 cut(s) 227
AspLEI GCGC 3 cut(s) 80, 119, 276
AspS9I GGNCC 2 cut(s) 131, 132
AsuHPI GGTGA 1 cut(s) 372
AxyI CCTNAGG 1 cut(s) 462
BaeGI GKGCMC 2 cut(s) 135, 349
BanII GRGCYC 1 cut(s) 135
BauI CACGAG 1 cut(s) 525
Bbv12I GWGCWC 2 cut(s) 349, 526
BbvI GCAGC 2 cut(s) 87, 454
BccI CCATC 4 cut(s) 56, 330, 335, 542
BcoDI GTCTC 1 cut(s) 134
BfoI RGCGCY 1 cut(s) 277
BglII AGATCT 1 cut(s) 158
BisI GCNGC 5 cut(s) 76, 290, 293, 468, 517
BlpI GCTNAGC 1 cut(s) 18
BlsI GCNGC 5 cut(s) 77, 291, 294, 469, 518
BmgBI CACGTC 2 cut(s) 90, 309
BmgT120I GGNCC 2 cut(s) 131, 132
BmiI GGNNCC 1 cut(s) 133
BmsI GCATC 1 cut(s) 361
Bpu1102I GCTNAGC 1 cut(s) 18
BsaBI GATNNNNATC 1 cut(s) 438
BsaI GGTCTC 1 cut(s) 134
BsaJI CCNNGG 1 cut(s) 146
BsaWI WCCGGW 2 cut(s) 64, 300
Bsc4I CCNNNNNNNGG 3 cut(s) 68, 129, 152
Bse21I CCTNAGG 1 cut(s) 462
Bse8I GATNNNNATC 1 cut(s) 438
BseAI TCCGGA 1 cut(s) 64
BseDI CCNNGG 1 cut(s) 146
BseGI GGATG 2 cut(s) 322, 376
BseJI GATNNNNATC 1 cut(s) 438
BseLI CCNNNNNNNGG 3 cut(s) 68, 129, 152
BseMII CTCAG 2 cut(s) 32, 453
BseSI GKGCMC 2 cut(s) 135, 349
BseXI GCAGC 2 cut(s) 87, 454
Bsh1236I CGCG 2 cut(s) 119, 295
BshFI GGCC 1 cut(s) 133
BsiHKAI GWGCWC 2 cut(s) 349, 526
BsiSI CCGG 3 cut(s) 25, 65, 301
BslFI GGGAC 1 cut(s) 69
BslI CCNNNNNNNGG 3 cut(s) 68, 129, 152
BsmAI GTCTC 1 cut(s) 134
BsmFI GGGAC 1 cut(s) 69
BsnI GGCC 1 cut(s) 133
Bso31I GGTCTC 1 cut(s) 134
Bsp120I GGGCCC 1 cut(s) 131
Bsp1286I GDGCHC 3 cut(s) 135, 349, 526
Bsp13I TCCGGA 1 cut(s) 64
Bsp143I GATC 4 cut(s) 5, 37, 158, 433
Bsp1720I GCTNAGC 1 cut(s) 18
BspACI CCGC 4 cut(s) 256, 290, 293, 517
BspANI GGCC 1 cut(s) 133
BspCNI CTCAG 2 cut(s) 31, 454
BspEI TCCGGA 1 cut(s) 64
BspFNI CGCG 2 cut(s) 119, 295
BspLI GGNNCC 1 cut(s) 133
BspPI GGATC 2 cut(s) 13, 428
BspTNI GGTCTC 1 cut(s) 134
BssECI CCNNGG 1 cut(s) 146
BssMI GATC 4 cut(s) 5, 37, 158, 433
BssSI CACGAG 1 cut(s) 525
BssT1I CCWWGG 1 cut(s) 146
Bst2BI CACGAG 1 cut(s) 525
Bst4CI ACNGT 1 cut(s) 351
BstDEI CTNAG 3 cut(s) 18, 462, 543
BstEII GGTNACC 1 cut(s) 378
BstENI CCTNNNNNAGG 1 cut(s) 150
BstF5I GGATG 2 cut(s) 322, 376
BstFNI CGCG 2 cut(s) 119, 295
BstH2I RGCGCY 1 cut(s) 277
BstHHI GCGC 3 cut(s) 80, 119, 276
BstKTI GATC 4 cut(s) 8, 40, 161, 436
BstMAI GTCTC 1 cut(s) 134
BstMBI GATC 4 cut(s) 5, 37, 158, 433
BstPI GGTNACC 1 cut(s) 378
BstSLI GKGCMC 2 cut(s) 135, 349
BstUI CGCG 2 cut(s) 119, 295
BstV1I GCAGC 2 cut(s) 87, 454
BstX2I RGATCY 1 cut(s) 158
BstYI RGATCY 1 cut(s) 158
Bsu36I CCTNAGG 1 cut(s) 462
BsuRI GGCC 1 cut(s) 133
BtrI CACGTC 2 cut(s) 90, 309
BtsCI GGATG 2 cut(s) 322, 376
BtsIMutI CAGTG 3 cut(s) 274, 347, 429
CfoI GCGC 3 cut(s) 80, 119, 276
Cfr13I GGNCC 2 cut(s) 131, 132
Csp6I GTAC 1 cut(s) 512
CspCI CAANNNNNGTGG 2 cut(s) 220, 255
CviAII CATG 6 cut(s) 31, 168, 338, 355, 375, 485
CviJI RGCY 7 cut(s) 22, 28, 61, 133, 289, 467, 497
CviKI_1 RGCY 7 cut(s) 22, 28, 61, 133, 289, 467, 497
CviQI GTAC 1 cut(s) 512
DdeI CTNAG 3 cut(s) 18, 462, 543
DpnI GATC 4 cut(s) 7, 39, 160, 435
DpnII GATC 4 cut(s) 5, 37, 158, 433
Eco130I CCWWGG 1 cut(s) 146
Eco24I GRGCYC 1 cut(s) 135
Eco31I GGTCTC 1 cut(s) 134
Eco47III AGCGCT 1 cut(s) 275
Eco57I CTGAAG 1 cut(s) 250
Eco81I CCTNAGG 1 cut(s) 462
Eco91I GGTNACC 1 cut(s) 378
EcoNI CCTNNNNNAGG 1 cut(s) 150
EcoO65I GGTNACC 1 cut(s) 378
EcoT14I CCWWGG 1 cut(s) 146
EcoT22I ATGCAT 1 cut(s) 376
EcoT38I GRGCYC 1 cut(s) 135
ErhI CCWWGG 1 cut(s) 146
FaeI CATG 6 cut(s) 34, 171, 341, 358, 378, 488
FaqI GGGAC 1 cut(s) 69
FatI CATG 6 cut(s) 30, 167, 337, 354, 374, 484
Fnu4HI GCNGC 5 cut(s) 76, 290, 293, 468, 517
FokI GGATG 2 cut(s) 309, 383
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 5 cut(s) 76, 290, 293, 468, 517
GlaI GCGC 3 cut(s) 79, 118, 275
GluI GCNGC 5 cut(s) 76, 290, 293, 468, 517
HaeII RGCGCY 1 cut(s) 277
HaeIII GGCC 1 cut(s) 133
HapII CCGG 3 cut(s) 25, 65, 301
HhaI GCGC 3 cut(s) 80, 119, 276
Hin1II CATG 6 cut(s) 34, 171, 341, 358, 378, 488
Hin6I GCGC 3 cut(s) 78, 117, 274
HinP1I GCGC 3 cut(s) 78, 117, 274
HincII GTYRAC 1 cut(s) 261
HindII GTYRAC 1 cut(s) 261
HindIII AAGCTT 1 cut(s) 495
HinfI GANTC 2 cut(s) 530, 539
HpaII CCGG 3 cut(s) 25, 65, 301
HphI GGTGA 1 cut(s) 372
Hpy166II GTNNAC 4 cut(s) 261, 306, 334, 347
Hpy188I TCNGA 3 cut(s) 37, 94, 313
Hpy188III TCNNGA 4 cut(s) 65, 281, 368, 527
Hpy8I GTNNAC 4 cut(s) 261, 306, 334, 347
HpyAV CCTTC 3 cut(s) 146, 274, 453
HpyCH4III ACNGT 1 cut(s) 351
HpyCH4IV ACGT 3 cut(s) 89, 308, 393
HpyCH4V TGCA 5 cut(s) 75, 347, 354, 374, 455
HpyF3I CTNAG 3 cut(s) 18, 462, 543
HpySE526I ACGT 3 cut(s) 89, 308, 393
Hsp92II CATG 6 cut(s) 34, 171, 341, 358, 378, 488
HspAI GCGC 3 cut(s) 78, 117, 274
Kpn2I TCCGGA 1 cut(s) 64
Kzo9I GATC 4 cut(s) 5, 37, 158, 433
LmnI GCTCC 2 cut(s) 27, 114
LpnPI CCDG 9 cut(s) 38, 78, 116, 222, 314, 353, 397, 465, 474
Lsp1109I GCAGC 2 cut(s) 87, 454
LweI GCATC 1 cut(s) 361
MaeII ACGT 3 cut(s) 89, 308, 393
MaeIII GTNAC 1 cut(s) 378
MalI GATC 4 cut(s) 7, 39, 160, 435
MboI GATC 4 cut(s) 5, 37, 158, 433
MflI RGATCY 1 cut(s) 158
MhlI GDGCHC 3 cut(s) 135, 349, 526
MluCI AATT 4 cut(s) 227, 232, 363, 402
MnlI CCTC 5 cut(s) 22, 61, 72, 457, 501
Mph1103I ATGCAT 1 cut(s) 376
MroI TCCGGA 1 cut(s) 64
MseI TTAA 3 cut(s) 185, 191, 407
MslI CAYNNNNRTG 2 cut(s) 327, 342
MspA1I CMGCKG 1 cut(s) 519
MspI CCGG 3 cut(s) 25, 65, 301
MvnI CGCG 2 cut(s) 119, 295
NdeII GATC 4 cut(s) 5, 37, 158, 433
NlaIII CATG 6 cut(s) 34, 171, 341, 358, 378, 488
NlaIV GGNNCC 1 cut(s) 133
NmuCI GTSAC 1 cut(s) 378
NsiI ATGCAT 1 cut(s) 376
PfeI GAWTC 1 cut(s) 530
PkrI GCNGC 5 cut(s) 77, 291, 294, 469, 518
PspEI GGTNACC 1 cut(s) 378
PspN4I GGNNCC 1 cut(s) 133
PspOMI GGGCCC 1 cut(s) 131
PspPI GGNCC 2 cut(s) 131, 132
PsuI RGATCY 1 cut(s) 158
RsaI GTAC 1 cut(s) 513
RsaNI GTAC 1 cut(s) 512
RseI CAYNNNNRTG 2 cut(s) 327, 342
SaqAI TTAA 3 cut(s) 185, 191, 407
SatI GCNGC 5 cut(s) 76, 290, 293, 468, 517
Sau3AI GATC 4 cut(s) 5, 37, 158, 433
Sau96I GGNCC 2 cut(s) 131, 132
SduI GDGCHC 3 cut(s) 135, 349, 526
SetI ASST 9 cut(s) 24, 92, 105, 157, 241, 266, 311, 396, 499
SfaNI GCATC 1 cut(s) 361
SmiMI CAYNNNNRTG 2 cut(s) 327, 342
Sse9I AATT 4 cut(s) 227, 232, 363, 402
SsiI CCGC 4 cut(s) 256, 290, 293, 517
StyI CCWWGG 1 cut(s) 146
TaaI ACNGT 1 cut(s) 351
TaiI ACGT 3 cut(s) 92, 311, 396
TaqI TCGA 1 cut(s) 537
TasI AATT 4 cut(s) 227, 232, 363, 402
TauI GCSGC 3 cut(s) 292, 295, 519
TfiI GAWTC 1 cut(s) 530
Tru1I TTAA 3 cut(s) 185, 191, 407
Tru9I TTAA 3 cut(s) 185, 191, 407
TscAI CASTG 3 cut(s) 274, 354, 436
TseFI GTSAC 1 cut(s) 378
TseI GCWGC 2 cut(s) 75, 467
Tsp45I GTSAC 1 cut(s) 378
TspRI CASTG 3 cut(s) 274, 354, 436
VneI GTGCAC 1 cut(s) 345
XagI CCTNNNNNAGG 1 cut(s) 150
XapI RAATTY 1 cut(s) 227
Zsp2I ATGCAT 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.