Rh2CG212700

Prokaryotic E2 family B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0014640
Physical Location & Seq
Reverse (-)
20927107 .. 20930276
3170 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG212700.1

Sequence Viewer

Length: 771 bp
ATGAATTTTTTTACCGCCAAAACCGCAAGCATTTACCCACCAAAACCCAAACCAGGGGGCTCAAAACACAAAGAGCCCACAGCCAAGGTTGACTCCACCAGCAAACAAATCTGGAAAAGGAATAGAATTTTACAGGTAGCAAAAGAAACACAGAGACAAACAGAGTCGGGTTTCCATTCCTTCCTTTCTTCTTTGTCAGATTTCAGCCAAAACGGGTTTCAGATTCCAAACCCCTTTCGTTCTCATCCACCACGACCACCACTCACCCATTCCCACCTTTTAAACTATAAACCAAAAAGAAAAAGCCAAAATCCAAATCCGCGTCTTCTTCGCTCTTTGGCTTTATTATATATACTCCGCCAGGTTGGTTTTGGGTTTCCAGAACAACAAGATCAACAACTCATCGAGCCTCTCTCTGCTATTATGGGATCGTCCTCTGCTCAGCTCCGGCTCATGTCCGATCTCAAAACCATCATAAACGAGCCTCCGGAGGGTTGCAGCGCAAGTCCTACGTCCGATGATAACCTGTTCGTGTGGAGCGCGACCATATTTGGGCCCGATGAGACCCCTTGGGAAGGTGGTGTTTTCAGTTTGCGATTGACCTTCAGTGAGCGCTATCCCGAAAAGCCGCCGCGTGTCCGGTTCACGTCCGAGATGTTCCATCCAAATGTTTACCATGATGGTGCACTGTGCATGGATATAATTCAGGATGCATGGTCACCCTGCCACAACGTATCAACAATTTTAACATCTGTTCAAGTAATTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

256

Amino Acids

28.95

Weight (kDa)

9.28

Isoelectric Point (pI)

52.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 150 - 255 1.9e-37 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014640)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18560 FvH4_1g18560 FvH4_1g18560
malus_domestica MD02G1193300.v1.1 MD15G1303600.v1.1
prunus_persica Prupe.6G213500_v2.0.a1
pyrus_communis pycom02g15700 pycom15g26650
rosa_chinensis RchiOBHm_Chr2g0109261
rosa_laevigata RLG00000017729
rosa_multiflora Rmu_sc0001807.1_g000008
rosa_roxburghii Rroxscaffold_2G00134350
rosa_rugosa Rorug02G0158900
rosa_samantha Rh2AG210300 Rh2BG220700 Rh2CG212700 Rh2DG216500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 322, 542, 634
AccIII TCCGGA 1 cut(s) 487
AciI CCGC 6 cut(s) 15, 24, 320, 358, 629, 632
AclWI GGATC 1 cut(s) 436
AcsI RAATTY 2 cut(s) 4, 126
AcuI CTGAAG 1 cut(s) 589
AfeI AGCGCT 1 cut(s) 614
AfiI CCNNNNNNNGG 5 cut(s) 53, 54, 491, 552, 575
AgsI TTSAA 1 cut(s) 758
AjiI CACGTC 1 cut(s) 648
AjnI CCWGG 2 cut(s) 52, 360
AjuI GAANNNNNNNTTGG 2 cut(s) 201, 233
AluBI AGCT 1 cut(s) 445
AluI AGCT 1 cut(s) 445
Alw21I GWGCWC 1 cut(s) 688
Alw26I GTCTC 2 cut(s) 148, 557
Alw44I GTGCAC 1 cut(s) 684
AlwI GGATC 1 cut(s) 436
Aor13HI TCCGGA 1 cut(s) 487
Aor51HI AGCGCT 1 cut(s) 614
AoxI GGCC 1 cut(s) 554
ApaI GGGCCC 1 cut(s) 558
ApaLI GTGCAC 1 cut(s) 684
ApeKI GCWGC 1 cut(s) 498
ApoI RAATTY 2 cut(s) 4, 126
AspLEI GCGC 3 cut(s) 503, 542, 615
AspS9I GGNCC 2 cut(s) 554, 555
AsuHPI GGTGA 2 cut(s) 256, 711
BaeGI GKGCMC 2 cut(s) 558, 688
BanII GRGCYC 3 cut(s) 62, 78, 558
BbsI GAAGAC 1 cut(s) 317
Bbv12I GWGCWC 1 cut(s) 688
BbvI GCAGC 1 cut(s) 510
BccI CCATC 3 cut(s) 479, 669, 674
BciT130I CCWGG 2 cut(s) 54, 362
BcoDI GTCTC 2 cut(s) 148, 557
BfoI RGCGCY 1 cut(s) 616
BisI GCNGC 3 cut(s) 499, 629, 632
BlpI GCTNAGC 1 cut(s) 441
BlsI GCNGC 3 cut(s) 500, 630, 633
Bme1390I CCNGG 2 cut(s) 54, 362
BmgBI CACGTC 1 cut(s) 648
BmgT120I GGNCC 2 cut(s) 554, 555
BmiI GGNNCC 1 cut(s) 556
BmrFI CCNGG 2 cut(s) 54, 362
BmsI GCATC 1 cut(s) 700
BpiI GAAGAC 1 cut(s) 317
BplI GAGNNNNNCTC 2 cut(s) 398, 430
Bpu1102I GCTNAGC 1 cut(s) 441
BsaI GGTCTC 1 cut(s) 557
BsaJI CCNNGG 3 cut(s) 53, 84, 569
BsaWI WCCGGW 2 cut(s) 487, 639
Bsc4I CCNNNNNNNGG 5 cut(s) 53, 54, 491, 552, 575
BseAI TCCGGA 1 cut(s) 487
BseBI CCWGG 2 cut(s) 54, 362
BseDI CCNNGG 3 cut(s) 53, 84, 569
BseGI GGATG 3 cut(s) 244, 661, 715
BseLI CCNNNNNNNGG 5 cut(s) 53, 54, 491, 552, 575
BseMII CTCAG 1 cut(s) 455
BseSI GKGCMC 2 cut(s) 558, 688
BseXI GCAGC 1 cut(s) 510
Bsh1236I CGCG 3 cut(s) 322, 542, 634
BshFI GGCC 1 cut(s) 556
BsiHKAI GWGCWC 1 cut(s) 688
BsiSI CCGG 3 cut(s) 448, 488, 640
BslI CCNNNNNNNGG 5 cut(s) 53, 54, 491, 552, 575
BsmAI GTCTC 2 cut(s) 148, 557
BsnI GGCC 1 cut(s) 556
Bso31I GGTCTC 1 cut(s) 557
Bsp120I GGGCCC 1 cut(s) 554
Bsp1286I GDGCHC 4 cut(s) 62, 78, 558, 688
Bsp13I TCCGGA 1 cut(s) 487
Bsp143I GATC 3 cut(s) 391, 428, 460
Bsp1720I GCTNAGC 1 cut(s) 441
BspACI CCGC 6 cut(s) 15, 24, 320, 358, 629, 632
BspANI GGCC 1 cut(s) 556
BspCNI CTCAG 1 cut(s) 454
BspEI TCCGGA 1 cut(s) 487
BspFNI CGCG 3 cut(s) 322, 542, 634
BspLI GGNNCC 1 cut(s) 556
BspPI GGATC 1 cut(s) 436
BspTNI GGTCTC 1 cut(s) 557
BssECI CCNNGG 3 cut(s) 53, 84, 569
BssMI GATC 3 cut(s) 391, 428, 460
BssT1I CCWWGG 2 cut(s) 84, 569
Bst2UI CCWGG 2 cut(s) 54, 362
Bst4CI ACNGT 1 cut(s) 690
BstC8I GCNNGC 1 cut(s) 28
BstDEI CTNAG 1 cut(s) 441
BstEII GGTNACC 1 cut(s) 717
BstENI CCTNNNNNAGG 1 cut(s) 573
BstF5I GGATG 3 cut(s) 244, 661, 715
BstFNI CGCG 3 cut(s) 322, 542, 634
BstH2I RGCGCY 1 cut(s) 616
BstHHI GCGC 3 cut(s) 503, 542, 615
BstKTI GATC 3 cut(s) 394, 431, 463
BstMAI GTCTC 2 cut(s) 148, 557
BstMBI GATC 3 cut(s) 391, 428, 460
BstMWI GCNNNNNNNGC 1 cut(s) 23
BstNI CCWGG 2 cut(s) 54, 362
BstPI GGTNACC 1 cut(s) 717
BstSCI CCNGG 2 cut(s) 52, 360
BstSLI GKGCMC 2 cut(s) 558, 688
BstUI CGCG 3 cut(s) 322, 542, 634
BstV1I GCAGC 1 cut(s) 510
BstV2I GAAGAC 1 cut(s) 317
BsuRI GGCC 1 cut(s) 556
BtrI CACGTC 1 cut(s) 648
BtsCI GGATG 3 cut(s) 244, 661, 715
BtsIMutI CAGTG 2 cut(s) 613, 686
Cac8I GCNNGC 1 cut(s) 28
CfoI GCGC 3 cut(s) 503, 542, 615
Cfr13I GGNCC 2 cut(s) 554, 555
CseI GACGC 1 cut(s) 311
CviAII CATG 4 cut(s) 454, 677, 694, 714
DdeI CTNAG 1 cut(s) 441
DpnI GATC 3 cut(s) 393, 430, 462
DpnII GATC 3 cut(s) 391, 428, 460
DraI TTTAAA 1 cut(s) 282
EciI GGCGGA 1 cut(s) 347
Eco130I CCWWGG 2 cut(s) 84, 569
Eco24I GRGCYC 3 cut(s) 62, 78, 558
Eco31I GGTCTC 1 cut(s) 557
Eco47III AGCGCT 1 cut(s) 614
Eco57I CTGAAG 1 cut(s) 589
Eco91I GGTNACC 1 cut(s) 717
EcoNI CCTNNNNNAGG 1 cut(s) 573
EcoO65I GGTNACC 1 cut(s) 717
EcoRII CCWGG 2 cut(s) 52, 360
EcoT14I CCWWGG 2 cut(s) 84, 569
EcoT22I ATGCAT 1 cut(s) 715
EcoT38I GRGCYC 3 cut(s) 62, 78, 558
ErhI CCWWGG 2 cut(s) 84, 569
FaeI CATG 4 cut(s) 457, 680, 697, 717
FatI CATG 4 cut(s) 453, 676, 693, 713
Fnu4HI GCNGC 3 cut(s) 499, 629, 632
FokI GGATG 3 cut(s) 231, 648, 722
FriOI GRGCYC 3 cut(s) 62, 78, 558
Fsp4HI GCNGC 3 cut(s) 499, 629, 632
GlaI GCGC 3 cut(s) 502, 541, 614
GluI GCNGC 3 cut(s) 499, 629, 632
HaeII RGCGCY 1 cut(s) 616
HaeIII GGCC 1 cut(s) 556
HapII CCGG 3 cut(s) 448, 488, 640
HgaI GACGC 1 cut(s) 311
HhaI GCGC 3 cut(s) 503, 542, 615
Hin1II CATG 4 cut(s) 457, 680, 697, 717
Hin6I GCGC 3 cut(s) 501, 540, 613
HinP1I GCGC 3 cut(s) 501, 540, 613
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HinfI GANTC 3 cut(s) 92, 164, 223
HpaII CCGG 3 cut(s) 448, 488, 640
HphI GGTGA 2 cut(s) 256, 711
Hpy166II GTNNAC 4 cut(s) 91, 645, 673, 686
Hpy188I TCNGA 5 cut(s) 199, 222, 460, 517, 652
Hpy188III TCNNGA 5 cut(s) 112, 380, 488, 620, 707
Hpy8I GTNNAC 4 cut(s) 91, 645, 673, 686
HpyAV CCTTC 3 cut(s) 190, 569, 613
HpyCH4III ACNGT 1 cut(s) 690
HpyCH4IV ACGT 3 cut(s) 512, 647, 732
HpyCH4V TGCA 4 cut(s) 498, 686, 693, 713
HpyF10VI GCNNNNNNNGC 1 cut(s) 23
HpyF3I CTNAG 1 cut(s) 441
HpySE526I ACGT 3 cut(s) 512, 647, 732
Hsp92II CATG 4 cut(s) 457, 680, 697, 717
HspAI GCGC 3 cut(s) 501, 540, 613
Kpn2I TCCGGA 1 cut(s) 487
Kzo9I GATC 3 cut(s) 391, 428, 460
LmnI GCTCC 2 cut(s) 450, 537
Lsp1109I GCAGC 1 cut(s) 510
LweI GCATC 1 cut(s) 700
MaeII ACGT 3 cut(s) 512, 647, 732
MaeIII GTNAC 1 cut(s) 717
MalI GATC 3 cut(s) 393, 430, 462
MboI GATC 3 cut(s) 391, 428, 460
MboII GAAGA 3 cut(s) 180, 317, 320
MhlI GDGCHC 4 cut(s) 62, 78, 558, 688
MluCI AATT 5 cut(s) 4, 126, 702, 741, 762
MlyI GAGTC 2 cut(s) 86, 173
MnlI CCTC 4 cut(s) 420, 445, 484, 495
Mph1103I ATGCAT 1 cut(s) 715
MroI TCCGGA 1 cut(s) 487
MseI TTAA 2 cut(s) 281, 746
MslI CAYNNNNRTG 2 cut(s) 666, 681
MspI CCGG 3 cut(s) 448, 488, 640
MspR9I CCNGG 2 cut(s) 54, 362
MvaI CCWGG 2 cut(s) 54, 362
MvnI CGCG 3 cut(s) 322, 542, 634
MwoI GCNNNNNNNGC 1 cut(s) 23
NdeII GATC 3 cut(s) 391, 428, 460
NlaIII CATG 4 cut(s) 457, 680, 697, 717
NlaIV GGNNCC 1 cut(s) 556
NmuCI GTSAC 1 cut(s) 717
NsiI ATGCAT 1 cut(s) 715
PfeI GAWTC 1 cut(s) 223
PkrI GCNGC 3 cut(s) 500, 630, 633
PleI GAGTC 2 cut(s) 86, 172
PpsI GAGTC 2 cut(s) 86, 172
Psp6I CCWGG 2 cut(s) 52, 360
PspEI GGTNACC 1 cut(s) 717
PspGI CCWGG 2 cut(s) 52, 360
PspN4I GGNNCC 1 cut(s) 556
PspOMI GGGCCC 1 cut(s) 554
PspPI GGNCC 2 cut(s) 554, 555
RseI CAYNNNNRTG 2 cut(s) 666, 681
SaqAI TTAA 2 cut(s) 281, 746
SatI GCNGC 3 cut(s) 499, 629, 632
Sau3AI GATC 3 cut(s) 391, 428, 460
Sau96I GGNCC 2 cut(s) 554, 555
SchI GAGTC 2 cut(s) 86, 173
ScrFI CCNGG 2 cut(s) 54, 362
SduI GDGCHC 4 cut(s) 62, 78, 558, 688
SfaNI GCATC 1 cut(s) 700
SmiMI CAYNNNNRTG 2 cut(s) 666, 681
Sse9I AATT 5 cut(s) 4, 126, 702, 741, 762
SsiI CCGC 6 cut(s) 15, 24, 320, 358, 629, 632
StyD4I CCNGG 2 cut(s) 52, 360
StyI CCWWGG 2 cut(s) 84, 569
TaaI ACNGT 1 cut(s) 690
TaiI ACGT 3 cut(s) 515, 650, 735
TaqI TCGA 1 cut(s) 405
TasI AATT 5 cut(s) 4, 126, 702, 741, 762
TauI GCSGC 2 cut(s) 631, 634
TfiI GAWTC 1 cut(s) 223
Tru1I TTAA 2 cut(s) 281, 746
Tru9I TTAA 2 cut(s) 281, 746
TscAI CASTG 2 cut(s) 613, 693
TseFI GTSAC 1 cut(s) 717
TseI GCWGC 1 cut(s) 498
Tsp45I GTSAC 1 cut(s) 717
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 613, 693
VneI GTGCAC 1 cut(s) 684
XagI CCTNNNNNAGG 1 cut(s) 573
XapI RAATTY 2 cut(s) 4, 126
XcmI CCANNNNNNNNNTGG 1 cut(s) 368
Zsp2I ATGCAT 1 cut(s) 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.