Rh2DG216500

Prokaryotic E2 family B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0014640
Physical Location & Seq
Reverse (-)
20748313 .. 20751509
3197 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG216500.1

Sequence Viewer

Length: 435 bp
ATGGGATCGTCCTCTGCTCAGCTCCGGCTCATGTCCGATCTCAAAACCATCATAAACGAGCCTCCGGAGGGTTGCAGCGCAAGTCCTACGTCCGATGATAACCTGTTCGTGTGGAGCGCGACCATATTTGGGCCCGATGAGACCCCTTGGGAAGGTGGTGTTTTCAGTTTGCGATTGACCTTCAGTGAGCGCTATCCCGAAAAGCCGCCGCGTGTCCGGTTCACGTCCGAGATGTTCCATCCAAATGTTTACCATGATGGTGCACTGTGCATGGATATAATTCAGGATGCATGGTCACCCTGCCACAACGTATCAACAATTTTAACATCTGTTCAATCACTTCTCACTGATCCAAATCCTTCAAGTCCTGCAAACCCTGAGGCTGCTCAAATGTATCAGCATGACATAAAAGCTTACAACAAGTGTGTACTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

144

Amino Acids

15.99

Weight (kDa)

4.75

Isoelectric Point (pI)

54.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 9 - 143 1.1e-49 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014640)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18560 FvH4_1g18560 FvH4_1g18560
malus_domestica MD02G1193300.v1.1 MD15G1303600.v1.1
prunus_persica Prupe.6G213500_v2.0.a1
pyrus_communis pycom02g15700 pycom15g26650
rosa_chinensis RchiOBHm_Chr2g0109261
rosa_laevigata RLG00000017729
rosa_multiflora Rmu_sc0001807.1_g000008
rosa_roxburghii Rroxscaffold_2G00134350
rosa_rugosa Rorug02G0158900
rosa_samantha Rh2AG210300 Rh2BG220700 Rh2CG212700 Rh2DG216500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 119, 211
AccIII TCCGGA 1 cut(s) 64
AciI CCGC 2 cut(s) 206, 209
AclWI GGATC 2 cut(s) 13, 344
AcuI CTGAAG 1 cut(s) 166
AfaI GTAC 1 cut(s) 429
AfeI AGCGCT 1 cut(s) 191
AfiI CCNNNNNNNGG 3 cut(s) 68, 129, 152
AgsI TTSAA 2 cut(s) 335, 363
AjiI CACGTC 1 cut(s) 225
AluBI AGCT 2 cut(s) 22, 413
AluI AGCT 2 cut(s) 22, 413
Alw21I GWGCWC 1 cut(s) 265
Alw26I GTCTC 1 cut(s) 134
Alw44I GTGCAC 1 cut(s) 261
AlwI GGATC 2 cut(s) 13, 344
Aor13HI TCCGGA 1 cut(s) 64
Aor51HI AGCGCT 1 cut(s) 191
AoxI GGCC 1 cut(s) 131
ApaI GGGCCC 1 cut(s) 135
ApaLI GTGCAC 1 cut(s) 261
ApeKI GCWGC 2 cut(s) 75, 383
AspLEI GCGC 3 cut(s) 80, 119, 192
AspS9I GGNCC 2 cut(s) 131, 132
AsuHPI GGTGA 1 cut(s) 288
AxyI CCTNAGG 1 cut(s) 378
BaeGI GKGCMC 2 cut(s) 135, 265
BanII GRGCYC 1 cut(s) 135
Bbv12I GWGCWC 1 cut(s) 265
BbvI GCAGC 2 cut(s) 87, 370
BccI CCATC 3 cut(s) 56, 246, 251
BcoDI GTCTC 1 cut(s) 134
BfoI RGCGCY 1 cut(s) 193
BisI GCNGC 4 cut(s) 76, 206, 209, 384
BlpI GCTNAGC 1 cut(s) 18
BlsI GCNGC 4 cut(s) 77, 207, 210, 385
BmgBI CACGTC 1 cut(s) 225
BmgT120I GGNCC 2 cut(s) 131, 132
BmiI GGNNCC 1 cut(s) 133
BmsI GCATC 1 cut(s) 277
Bpu1102I GCTNAGC 1 cut(s) 18
BsaBI GATNNNNATC 1 cut(s) 354
BsaI GGTCTC 1 cut(s) 134
BsaJI CCNNGG 1 cut(s) 146
BsaWI WCCGGW 2 cut(s) 64, 216
Bsc4I CCNNNNNNNGG 3 cut(s) 68, 129, 152
Bse21I CCTNAGG 1 cut(s) 378
Bse8I GATNNNNATC 1 cut(s) 354
BseAI TCCGGA 1 cut(s) 64
BseDI CCNNGG 1 cut(s) 146
BseGI GGATG 2 cut(s) 238, 292
BseJI GATNNNNATC 1 cut(s) 354
BseLI CCNNNNNNNGG 3 cut(s) 68, 129, 152
BseMII CTCAG 2 cut(s) 32, 369
BseSI GKGCMC 2 cut(s) 135, 265
BseXI GCAGC 2 cut(s) 87, 370
Bsh1236I CGCG 2 cut(s) 119, 211
BshFI GGCC 1 cut(s) 133
BsiHKAI GWGCWC 1 cut(s) 265
BsiSI CCGG 3 cut(s) 25, 65, 217
BslI CCNNNNNNNGG 3 cut(s) 68, 129, 152
BsmAI GTCTC 1 cut(s) 134
BsnI GGCC 1 cut(s) 133
Bso31I GGTCTC 1 cut(s) 134
Bsp120I GGGCCC 1 cut(s) 131
Bsp1286I GDGCHC 2 cut(s) 135, 265
Bsp13I TCCGGA 1 cut(s) 64
Bsp143I GATC 3 cut(s) 5, 37, 349
Bsp1720I GCTNAGC 1 cut(s) 18
BspACI CCGC 2 cut(s) 206, 209
BspANI GGCC 1 cut(s) 133
BspCNI CTCAG 2 cut(s) 31, 370
BspEI TCCGGA 1 cut(s) 64
BspFNI CGCG 2 cut(s) 119, 211
BspLI GGNNCC 1 cut(s) 133
BspPI GGATC 2 cut(s) 13, 344
BspTNI GGTCTC 1 cut(s) 134
BssECI CCNNGG 1 cut(s) 146
BssMI GATC 3 cut(s) 5, 37, 349
BssT1I CCWWGG 1 cut(s) 146
Bst4CI ACNGT 1 cut(s) 267
BstDEI CTNAG 2 cut(s) 18, 378
BstEII GGTNACC 1 cut(s) 294
BstENI CCTNNNNNAGG 1 cut(s) 150
BstF5I GGATG 2 cut(s) 238, 292
BstFNI CGCG 2 cut(s) 119, 211
BstH2I RGCGCY 1 cut(s) 193
BstHHI GCGC 3 cut(s) 80, 119, 192
BstKTI GATC 3 cut(s) 8, 40, 352
BstMAI GTCTC 1 cut(s) 134
BstMBI GATC 3 cut(s) 5, 37, 349
BstPI GGTNACC 1 cut(s) 294
BstSLI GKGCMC 2 cut(s) 135, 265
BstUI CGCG 2 cut(s) 119, 211
BstV1I GCAGC 2 cut(s) 87, 370
Bsu36I CCTNAGG 1 cut(s) 378
BsuRI GGCC 1 cut(s) 133
BtrI CACGTC 1 cut(s) 225
BtsCI GGATG 2 cut(s) 238, 292
BtsIMutI CAGTG 3 cut(s) 190, 263, 345
CfoI GCGC 3 cut(s) 80, 119, 192
Cfr13I GGNCC 2 cut(s) 131, 132
Csp6I GTAC 1 cut(s) 428
CviAII CATG 5 cut(s) 31, 254, 271, 291, 401
CviJI RGCY 7 cut(s) 22, 28, 61, 133, 205, 383, 413
CviKI_1 RGCY 7 cut(s) 22, 28, 61, 133, 205, 383, 413
CviQI GTAC 1 cut(s) 428
DdeI CTNAG 2 cut(s) 18, 378
DpnI GATC 3 cut(s) 7, 39, 351
DpnII GATC 3 cut(s) 5, 37, 349
Eco130I CCWWGG 1 cut(s) 146
Eco24I GRGCYC 1 cut(s) 135
Eco31I GGTCTC 1 cut(s) 134
Eco47III AGCGCT 1 cut(s) 191
Eco57I CTGAAG 1 cut(s) 166
Eco81I CCTNAGG 1 cut(s) 378
Eco91I GGTNACC 1 cut(s) 294
EcoNI CCTNNNNNAGG 1 cut(s) 150
EcoO65I GGTNACC 1 cut(s) 294
EcoT14I CCWWGG 1 cut(s) 146
EcoT22I ATGCAT 1 cut(s) 292
EcoT38I GRGCYC 1 cut(s) 135
ErhI CCWWGG 1 cut(s) 146
FaeI CATG 5 cut(s) 34, 257, 274, 294, 404
FaiI YATR 9 cut(s) 32, 53, 125, 255, 272, 278, 292, 402, 407
FatI CATG 5 cut(s) 30, 253, 270, 290, 400
Fnu4HI GCNGC 4 cut(s) 76, 206, 209, 384
FokI GGATG 2 cut(s) 225, 299
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 4 cut(s) 76, 206, 209, 384
GlaI GCGC 3 cut(s) 79, 118, 191
GluI GCNGC 4 cut(s) 76, 206, 209, 384
HaeII RGCGCY 1 cut(s) 193
HaeIII GGCC 1 cut(s) 133
HapII CCGG 3 cut(s) 25, 65, 217
HhaI GCGC 3 cut(s) 80, 119, 192
Hin1II CATG 5 cut(s) 34, 257, 274, 294, 404
Hin6I GCGC 3 cut(s) 78, 117, 190
HinP1I GCGC 3 cut(s) 78, 117, 190
HindIII AAGCTT 1 cut(s) 411
HpaII CCGG 3 cut(s) 25, 65, 217
HphI GGTGA 1 cut(s) 288
Hpy166II GTNNAC 4 cut(s) 222, 250, 263, 428
Hpy188I TCNGA 3 cut(s) 37, 94, 229
Hpy188III TCNNGA 3 cut(s) 65, 197, 284
Hpy8I GTNNAC 4 cut(s) 222, 250, 263, 428
HpyAV CCTTC 3 cut(s) 146, 190, 369
HpyCH4III ACNGT 1 cut(s) 267
HpyCH4IV ACGT 3 cut(s) 89, 224, 309
HpyCH4V TGCA 5 cut(s) 75, 263, 270, 290, 371
HpyF3I CTNAG 2 cut(s) 18, 378
HpySE526I ACGT 3 cut(s) 89, 224, 309
Hsp92II CATG 5 cut(s) 34, 257, 274, 294, 404
HspAI GCGC 3 cut(s) 78, 117, 190
Kpn2I TCCGGA 1 cut(s) 64
Kzo9I GATC 3 cut(s) 5, 37, 349
LmnI GCTCC 2 cut(s) 27, 114
LpnPI CCDG 8 cut(s) 38, 78, 116, 230, 269, 313, 381, 390
Lsp1109I GCAGC 2 cut(s) 87, 370
LweI GCATC 1 cut(s) 277
MaeII ACGT 3 cut(s) 89, 224, 309
MaeIII GTNAC 1 cut(s) 294
MalI GATC 3 cut(s) 7, 39, 351
MboI GATC 3 cut(s) 5, 37, 349
MhlI GDGCHC 2 cut(s) 135, 265
MluCI AATT 2 cut(s) 279, 318
MnlI CCTC 4 cut(s) 22, 61, 72, 373
Mph1103I ATGCAT 1 cut(s) 292
MroI TCCGGA 1 cut(s) 64
MseI TTAA 1 cut(s) 323
MslI CAYNNNNRTG 2 cut(s) 243, 258
MspI CCGG 3 cut(s) 25, 65, 217
MvnI CGCG 2 cut(s) 119, 211
NdeII GATC 3 cut(s) 5, 37, 349
NlaIII CATG 5 cut(s) 34, 257, 274, 294, 404
NlaIV GGNNCC 1 cut(s) 133
NmuCI GTSAC 1 cut(s) 294
NsiI ATGCAT 1 cut(s) 292
PkrI GCNGC 4 cut(s) 77, 207, 210, 385
PspEI GGTNACC 1 cut(s) 294
PspN4I GGNNCC 1 cut(s) 133
PspOMI GGGCCC 1 cut(s) 131
PspPI GGNCC 2 cut(s) 131, 132
RsaI GTAC 1 cut(s) 429
RsaNI GTAC 1 cut(s) 428
RseI CAYNNNNRTG 2 cut(s) 243, 258
SaqAI TTAA 1 cut(s) 323
SatI GCNGC 4 cut(s) 76, 206, 209, 384
Sau3AI GATC 3 cut(s) 5, 37, 349
Sau96I GGNCC 2 cut(s) 131, 132
SduI GDGCHC 2 cut(s) 135, 265
SetI ASST 8 cut(s) 24, 92, 105, 157, 182, 227, 312, 415
SfaNI GCATC 1 cut(s) 277
SmiMI CAYNNNNRTG 2 cut(s) 243, 258
Sse9I AATT 2 cut(s) 279, 318
SsiI CCGC 2 cut(s) 206, 209
StyI CCWWGG 1 cut(s) 146
TaaI ACNGT 1 cut(s) 267
TaiI ACGT 3 cut(s) 92, 227, 312
TasI AATT 2 cut(s) 279, 318
TatI WGTACW 1 cut(s) 427
TauI GCSGC 2 cut(s) 208, 211
Tru1I TTAA 1 cut(s) 323
Tru9I TTAA 1 cut(s) 323
TscAI CASTG 3 cut(s) 190, 270, 352
TseFI GTSAC 1 cut(s) 294
TseI GCWGC 2 cut(s) 75, 383
Tsp45I GTSAC 1 cut(s) 294
TspRI CASTG 3 cut(s) 190, 270, 352
VneI GTGCAC 1 cut(s) 261
XagI CCTNNNNNAGG 1 cut(s) 150
Zsp2I ATGCAT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.