Rmu_sc0001807.1_g000008

Prokaryotic E2 family B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001807.1
OG0014640
Physical Location & Seq
Reverse (-)
25983 .. 28708
2726 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001807.1_g000008.1.cds

Sequence Viewer

Length: 894 bp
atgaattcttttaccgccaaaaccgcaagcatttacccaccaaaacccaaaccagggggctcaaaacacaaagagcccacagctaaggttgactccaccagcaaacaaatctggaagaggaatagaattttacaggtagcaaaagaaacacagagacaaacagagtcgggtttccattccttcctttcttctttgtcagatttccgccaaaacgggtttcagattccaaacccctttcgttctcatccaccacccaccacccaccaccactcacccatcccccaccttttaaactataaaccaaaaagaaaaagccaaaatccaaatccgcgtcttcttcgctctttggctttatcatatatactccgccagattggttttgggtttccagaacaacaagatcaacaactcatcgagcctctctctgctattatgggatcgtcctctgctcagctccggctcatgtccgatctcaaaaccatcataaacgagcctccggagggttgcagcgcaagtcctacgtccgatgataacctgttcgtgtggagcgcgaccatatttgggcccgatgagaccccttgggaaggtggtgttttcagtttgcgattgaccttcagtgagcgctatcccgaaaagccgccgcgtgtccggttcacgtccgagatgttccatccaaatgtttaccatgatggtgcactgtgcatggatataattcaggatgcatggtcaccctgccacaacgtatcaacaattttaacatctgttcaatcacttctcactgatccaaatccttcaagtcctgcaaaccctgaggctgctcaaatgtatcagcatgacataaaagcttacaacaagagggtacgccgctgtgctcgtgattccatcgagtcttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

297

Amino Acids

33.52

Weight (kDa)

9.24

Isoelectric Point (pI)

61.38

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014640)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18560 FvH4_1g18560 FvH4_1g18560
malus_domestica MD02G1193300.v1.1 MD15G1303600.v1.1
prunus_persica Prupe.6G213500_v2.0.a1
pyrus_communis pycom02g15700 pycom15g26650
rosa_chinensis RchiOBHm_Chr2g0109261
rosa_laevigata RLG00000017729
rosa_multiflora Rmu_sc0001807.1_g000008
rosa_roxburghii Rroxscaffold_2G00134350
rosa_rugosa Rorug02G0158900
rosa_samantha Rh2AG210300 Rh2BG220700 Rh2CG212700 Rh2DG216500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 331, 551, 643
AccIII TCCGGA 1 cut(s) 496
AciI CCGC 8 cut(s) 15, 24, 205, 329, 367, 638, 641, 865
AclWI GGATC 2 cut(s) 445, 776
AcsI RAATTY 2 cut(s) 4, 126
AcuI CTGAAG 1 cut(s) 598
AfaI GTAC 1 cut(s) 861
AfeI AGCGCT 1 cut(s) 623
AfiI CCNNNNNNNGG 5 cut(s) 53, 54, 500, 561, 584
AgsI TTSAA 2 cut(s) 767, 795
AjiI CACGTC 1 cut(s) 657
AjnI CCWGG 1 cut(s) 52
AjuI GAANNNNNNNTTGG 2 cut(s) 201, 233
AluBI AGCT 3 cut(s) 83, 454, 845
AluI AGCT 3 cut(s) 83, 454, 845
Alw21I GWGCWC 2 cut(s) 697, 874
Alw26I GTCTC 2 cut(s) 148, 566
Alw44I GTGCAC 1 cut(s) 693
AlwI GGATC 2 cut(s) 445, 776
Aor13HI TCCGGA 1 cut(s) 496
Aor51HI AGCGCT 1 cut(s) 623
AoxI GGCC 1 cut(s) 563
ApaI GGGCCC 1 cut(s) 567
ApaLI GTGCAC 1 cut(s) 693
ApeKI GCWGC 2 cut(s) 507, 815
ApoI RAATTY 2 cut(s) 4, 126
AspLEI GCGC 3 cut(s) 512, 551, 624
AspS9I GGNCC 2 cut(s) 563, 564
AsuHPI GGTGA 2 cut(s) 264, 720
AxyI CCTNAGG 1 cut(s) 810
BaeGI GKGCMC 2 cut(s) 567, 697
BanII GRGCYC 3 cut(s) 62, 78, 567
BauI CACGAG 1 cut(s) 873
BbsI GAAGAC 1 cut(s) 326
Bbv12I GWGCWC 2 cut(s) 697, 874
BbvI GCAGC 2 cut(s) 519, 802
BccI CCATC 5 cut(s) 284, 488, 678, 683, 890
BciT130I CCWGG 1 cut(s) 54
BcoDI GTCTC 2 cut(s) 148, 566
BfoI RGCGCY 1 cut(s) 625
BisI GCNGC 5 cut(s) 508, 638, 641, 816, 865
BlpI GCTNAGC 1 cut(s) 450
BlsI GCNGC 5 cut(s) 509, 639, 642, 817, 866
Bme1390I CCNGG 1 cut(s) 54
BmgBI CACGTC 1 cut(s) 657
BmgT120I GGNCC 2 cut(s) 563, 564
BmiI GGNNCC 1 cut(s) 565
BmrFI CCNGG 1 cut(s) 54
BmsI GCATC 1 cut(s) 709
BpiI GAAGAC 1 cut(s) 326
BplI GAGNNNNNCTC 2 cut(s) 407, 439
Bpu10I CCTNAGC 1 cut(s) 84
Bpu1102I GCTNAGC 1 cut(s) 450
BsaBI GATNNNNATC 1 cut(s) 786
BsaI GGTCTC 1 cut(s) 566
BsaJI CCNNGG 2 cut(s) 53, 578
BsaWI WCCGGW 2 cut(s) 496, 648
Bsc4I CCNNNNNNNGG 5 cut(s) 53, 54, 500, 561, 584
Bse21I CCTNAGG 1 cut(s) 810
Bse8I GATNNNNATC 1 cut(s) 786
BseAI TCCGGA 1 cut(s) 496
BseBI CCWGG 1 cut(s) 54
BseDI CCNNGG 2 cut(s) 53, 578
BseGI GGATG 4 cut(s) 244, 276, 670, 724
BseJI GATNNNNATC 1 cut(s) 786
BseLI CCNNNNNNNGG 5 cut(s) 53, 54, 500, 561, 584
BseMII CTCAG 2 cut(s) 464, 801
BseSI GKGCMC 2 cut(s) 567, 697
BseXI GCAGC 2 cut(s) 519, 802
Bsh1236I CGCG 3 cut(s) 331, 551, 643
BshFI GGCC 1 cut(s) 565
BsiHKAI GWGCWC 2 cut(s) 697, 874
BsiSI CCGG 3 cut(s) 457, 497, 649
BslI CCNNNNNNNGG 5 cut(s) 53, 54, 500, 561, 584
BsmAI GTCTC 2 cut(s) 148, 566
BsnI GGCC 1 cut(s) 565
Bso31I GGTCTC 1 cut(s) 566
Bsp120I GGGCCC 1 cut(s) 563
Bsp1286I GDGCHC 5 cut(s) 62, 78, 567, 697, 874
Bsp13I TCCGGA 1 cut(s) 496
Bsp143I GATC 4 cut(s) 400, 437, 469, 781
Bsp1720I GCTNAGC 1 cut(s) 450
BspACI CCGC 8 cut(s) 15, 24, 205, 329, 367, 638, 641, 865
BspANI GGCC 1 cut(s) 565
BspCNI CTCAG 2 cut(s) 463, 802
BspEI TCCGGA 1 cut(s) 496
BspFNI CGCG 3 cut(s) 331, 551, 643
BspLI GGNNCC 1 cut(s) 565
BspPI GGATC 2 cut(s) 445, 776
BspTNI GGTCTC 1 cut(s) 566
BssECI CCNNGG 2 cut(s) 53, 578
BssMI GATC 4 cut(s) 400, 437, 469, 781
BssSI CACGAG 1 cut(s) 873
BssT1I CCWWGG 1 cut(s) 578
Bst2BI CACGAG 1 cut(s) 873
Bst2UI CCWGG 1 cut(s) 54
Bst4CI ACNGT 1 cut(s) 699
Bst6I CTCTTC 1 cut(s) 110
BstC8I GCNNGC 1 cut(s) 28
BstDEI CTNAG 4 cut(s) 84, 450, 810, 891
BstEII GGTNACC 1 cut(s) 726
BstENI CCTNNNNNAGG 1 cut(s) 582
BstF5I GGATG 4 cut(s) 244, 276, 670, 724
BstFNI CGCG 3 cut(s) 331, 551, 643
BstH2I RGCGCY 1 cut(s) 625
BstHHI GCGC 3 cut(s) 512, 551, 624
BstKTI GATC 4 cut(s) 403, 440, 472, 784
BstMAI GTCTC 2 cut(s) 148, 566
BstMBI GATC 4 cut(s) 400, 437, 469, 781
BstMWI GCNNNNNNNGC 1 cut(s) 23
BstNI CCWGG 1 cut(s) 54
BstPI GGTNACC 1 cut(s) 726
BstSCI CCNGG 1 cut(s) 52
BstSLI GKGCMC 2 cut(s) 567, 697
BstUI CGCG 3 cut(s) 331, 551, 643
BstV1I GCAGC 2 cut(s) 519, 802
BstV2I GAAGAC 1 cut(s) 326
Bsu36I CCTNAGG 1 cut(s) 810
BsuRI GGCC 1 cut(s) 565
BtrI CACGTC 1 cut(s) 657
BtsCI GGATG 4 cut(s) 244, 276, 670, 724
BtsIMutI CAGTG 3 cut(s) 622, 695, 777
Cac8I GCNNGC 1 cut(s) 28
CfoI GCGC 3 cut(s) 512, 551, 624
Cfr13I GGNCC 2 cut(s) 563, 564
CseI GACGC 1 cut(s) 320
Csp6I GTAC 1 cut(s) 860
CviAII CATG 5 cut(s) 463, 686, 703, 723, 833
CviQI GTAC 1 cut(s) 860
DdeI CTNAG 4 cut(s) 84, 450, 810, 891
DpnI GATC 4 cut(s) 402, 439, 471, 783
DpnII GATC 4 cut(s) 400, 437, 469, 781
DraI TTTAAA 1 cut(s) 291
Eam1104I CTCTTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 110
EciI GGCGGA 2 cut(s) 194, 356
Eco130I CCWWGG 1 cut(s) 578
Eco24I GRGCYC 3 cut(s) 62, 78, 567
Eco31I GGTCTC 1 cut(s) 566
Eco47III AGCGCT 1 cut(s) 623
Eco57I CTGAAG 1 cut(s) 598
Eco81I CCTNAGG 1 cut(s) 810
Eco91I GGTNACC 1 cut(s) 726
EcoNI CCTNNNNNAGG 1 cut(s) 582
EcoO65I GGTNACC 1 cut(s) 726
EcoRI GAATTC 1 cut(s) 4
EcoRII CCWGG 1 cut(s) 52
EcoT14I CCWWGG 1 cut(s) 578
EcoT22I ATGCAT 1 cut(s) 724
EcoT38I GRGCYC 3 cut(s) 62, 78, 567
ErhI CCWWGG 1 cut(s) 578
FaeI CATG 5 cut(s) 466, 689, 706, 726, 836
FatI CATG 5 cut(s) 462, 685, 702, 722, 832
Fnu4HI GCNGC 5 cut(s) 508, 638, 641, 816, 865
FokI GGATG 4 cut(s) 231, 263, 657, 731
FriOI GRGCYC 3 cut(s) 62, 78, 567
Fsp4HI GCNGC 5 cut(s) 508, 638, 641, 816, 865
GlaI GCGC 3 cut(s) 511, 550, 623
GluI GCNGC 5 cut(s) 508, 638, 641, 816, 865
HaeII RGCGCY 1 cut(s) 625
HaeIII GGCC 1 cut(s) 565
HapII CCGG 3 cut(s) 457, 497, 649
HgaI GACGC 1 cut(s) 320
HhaI GCGC 3 cut(s) 512, 551, 624
Hin1II CATG 5 cut(s) 466, 689, 706, 726, 836
Hin6I GCGC 3 cut(s) 510, 549, 622
HinP1I GCGC 3 cut(s) 510, 549, 622
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HindIII AAGCTT 1 cut(s) 843
HinfI GANTC 5 cut(s) 92, 164, 223, 878, 887
HpaII CCGG 3 cut(s) 457, 497, 649
HphI GGTGA 2 cut(s) 264, 720
Hpy166II GTNNAC 4 cut(s) 91, 654, 682, 695
Hpy188I TCNGA 5 cut(s) 199, 222, 469, 526, 661
Hpy188III TCNNGA 6 cut(s) 112, 389, 497, 629, 716, 875
Hpy8I GTNNAC 4 cut(s) 91, 654, 682, 695
HpyAV CCTTC 4 cut(s) 190, 578, 622, 801
HpyCH4III ACNGT 1 cut(s) 699
HpyCH4IV ACGT 3 cut(s) 521, 656, 741
HpyCH4V TGCA 5 cut(s) 507, 695, 702, 722, 803
HpyF10VI GCNNNNNNNGC 1 cut(s) 23
HpyF3I CTNAG 4 cut(s) 84, 450, 810, 891
HpySE526I ACGT 3 cut(s) 521, 656, 741
Hsp92II CATG 5 cut(s) 466, 689, 706, 726, 836
HspAI GCGC 3 cut(s) 510, 549, 622
Kpn2I TCCGGA 1 cut(s) 496
Kzo9I GATC 4 cut(s) 400, 437, 469, 781
LmnI GCTCC 2 cut(s) 459, 546
Lsp1109I GCAGC 2 cut(s) 519, 802
LweI GCATC 1 cut(s) 709
MaeII ACGT 3 cut(s) 521, 656, 741
MaeIII GTNAC 1 cut(s) 726
MalI GATC 4 cut(s) 402, 439, 471, 783
MboI GATC 4 cut(s) 400, 437, 469, 781
MboII GAAGA 4 cut(s) 127, 180, 326, 329
MhlI GDGCHC 5 cut(s) 62, 78, 567, 697, 874
MluCI AATT 4 cut(s) 4, 126, 711, 750
MlyI GAGTC 2 cut(s) 86, 173
MnlI CCTC 7 cut(s) 111, 429, 454, 493, 504, 805, 849
Mph1103I ATGCAT 1 cut(s) 724
MroI TCCGGA 1 cut(s) 496
MseI TTAA 2 cut(s) 290, 755
MslI CAYNNNNRTG 2 cut(s) 675, 690
MspA1I CMGCKG 1 cut(s) 867
MspI CCGG 3 cut(s) 457, 497, 649
MspR9I CCNGG 1 cut(s) 54
MvaI CCWGG 1 cut(s) 54
MvnI CGCG 3 cut(s) 331, 551, 643
MwoI GCNNNNNNNGC 1 cut(s) 23
NdeII GATC 4 cut(s) 400, 437, 469, 781
NlaIII CATG 5 cut(s) 466, 689, 706, 726, 836
NlaIV GGNNCC 1 cut(s) 565
NmuCI GTSAC 1 cut(s) 726
NsiI ATGCAT 1 cut(s) 724
PfeI GAWTC 2 cut(s) 223, 878
PkrI GCNGC 5 cut(s) 509, 639, 642, 817, 866
PleI GAGTC 2 cut(s) 86, 172
PpsI GAGTC 2 cut(s) 86, 172
Psp6I CCWGG 1 cut(s) 52
PspEI GGTNACC 1 cut(s) 726
PspGI CCWGG 1 cut(s) 52
PspN4I GGNNCC 1 cut(s) 565
PspOMI GGGCCC 1 cut(s) 563
PspPI GGNCC 2 cut(s) 563, 564
RsaI GTAC 1 cut(s) 861
RsaNI GTAC 1 cut(s) 860
RseI CAYNNNNRTG 2 cut(s) 675, 690
SaqAI TTAA 2 cut(s) 290, 755
SatI GCNGC 5 cut(s) 508, 638, 641, 816, 865
Sau3AI GATC 4 cut(s) 400, 437, 469, 781
Sau96I GGNCC 2 cut(s) 563, 564
SchI GAGTC 2 cut(s) 86, 173
ScrFI CCNGG 1 cut(s) 54
SduI GDGCHC 5 cut(s) 62, 78, 567, 697, 874
SfaNI GCATC 1 cut(s) 709
SmiMI CAYNNNNRTG 2 cut(s) 675, 690
Sse9I AATT 4 cut(s) 4, 126, 711, 750
SsiI CCGC 8 cut(s) 15, 24, 205, 329, 367, 638, 641, 865
StyD4I CCNGG 1 cut(s) 52
StyI CCWWGG 1 cut(s) 578
TaaI ACNGT 1 cut(s) 699
TaiI ACGT 3 cut(s) 524, 659, 744
TaqI TCGA 2 cut(s) 414, 885
TasI AATT 4 cut(s) 4, 126, 711, 750
TauI GCSGC 3 cut(s) 640, 643, 867
TfiI GAWTC 2 cut(s) 223, 878
Tru1I TTAA 2 cut(s) 290, 755
Tru9I TTAA 2 cut(s) 290, 755
TscAI CASTG 3 cut(s) 622, 702, 784
TseFI GTSAC 1 cut(s) 726
TseI GCWGC 2 cut(s) 507, 815
Tsp45I GTSAC 1 cut(s) 726
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 3 cut(s) 622, 702, 784
VneI GTGCAC 1 cut(s) 693
XagI CCTNNNNNAGG 1 cut(s) 582
XapI RAATTY 2 cut(s) 4, 126
XcmI CCANNNNNNNNNTGG 1 cut(s) 377
Zsp2I ATGCAT 1 cut(s) 724
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.