Rroxscaffold_2G00134350

Prokaryotic E2 family B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014640
Physical Location & Seq
Forward (+)
71836157 .. 71839056
2900 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00134350.1

Sequence Viewer

Length: 546 bp
ATGGGATCGTCCTCTGCTCAGCTCCGGCTCATGTCCGATCTCAAAACCATCATAAACGAGCCTCCGGAGGGTTGCAGCGCAAGTCCTACGTCCGATGATAACCTCTTCGTGTGGAGCGCGACCATATTTGGGCCCGATGAGACCCCTTGGGAAGGTGGTGTTTTCAGTTTGCGATTGACCTTCAGTGAGCGCTATCCCGAAAAGCCGCCGCGTGTCCGGTTCACTTTCCAGTTGAGTTTGAAATTTGATGCGGATGGAATTGTGTTAGTGTTGTTAGTTTTAGAGTTCACCATTAGTAACTTAGCAGCATATGATTATCGGAGAATCCATCTGCCTCTAGAGTTTGAAGTGTTGAAGTTTTACCATGATGGTGCACTGTGCATGGATATAATTCAGGATGCATGGTCACCCTGCCACAACTCACTTCTCACTGATCCAAATCCTTCAAGTCCTGCAAACCCTGAGGCTGCTCAAATGTATCAGCATGACATGAAAGCTTACAACAAGAGGGTACGCCGCTGTGCTCGTGATTCCATCGAGTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

181

Amino Acids

20.51

Weight (kDa)

5.05

Isoelectric Point (pI)

57.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 9 - 77 2.4e-19 Ubiquitin-conjugating enzyme
UQ_con PF00179 121 - 174 4.4e-09 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014640)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18560 FvH4_1g18560 FvH4_1g18560
malus_domestica MD02G1193300.v1.1 MD15G1303600.v1.1
prunus_persica Prupe.6G213500_v2.0.a1
pyrus_communis pycom02g15700 pycom15g26650
rosa_chinensis RchiOBHm_Chr2g0109261
rosa_laevigata RLG00000017729
rosa_multiflora Rmu_sc0001807.1_g000008
rosa_roxburghii Rroxscaffold_2G00134350
rosa_rugosa Rorug02G0158900
rosa_samantha Rh2AG210300 Rh2BG220700 Rh2CG212700 Rh2DG216500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 119, 211
AccIII TCCGGA 1 cut(s) 64
AciI CCGC 4 cut(s) 206, 209, 251, 517
AclWI GGATC 2 cut(s) 13, 428
AcsI RAATTY 1 cut(s) 242
AcuI CTGAAG 1 cut(s) 166
AfaI GTAC 1 cut(s) 513
AfeI AGCGCT 1 cut(s) 191
AfiI CCNNNNNNNGG 3 cut(s) 68, 129, 152
AgsI TTSAA 4 cut(s) 241, 347, 355, 447
AluBI AGCT 2 cut(s) 22, 497
AluI AGCT 2 cut(s) 22, 497
Alw21I GWGCWC 2 cut(s) 376, 526
Alw26I GTCTC 1 cut(s) 134
Alw44I GTGCAC 1 cut(s) 372
AlwI GGATC 2 cut(s) 13, 428
Aor13HI TCCGGA 1 cut(s) 64
Aor51HI AGCGCT 1 cut(s) 191
AoxI GGCC 1 cut(s) 131
ApaI GGGCCC 1 cut(s) 135
ApaLI GTGCAC 1 cut(s) 372
ApeKI GCWGC 3 cut(s) 75, 305, 467
ApoI RAATTY 1 cut(s) 242
AspLEI GCGC 3 cut(s) 80, 119, 192
AspS9I GGNCC 2 cut(s) 131, 132
AsuHPI GGTGA 2 cut(s) 280, 399
AxyI CCTNAGG 1 cut(s) 462
BaeGI GKGCMC 2 cut(s) 135, 376
BanII GRGCYC 1 cut(s) 135
BauI CACGAG 1 cut(s) 525
Bbv12I GWGCWC 2 cut(s) 376, 526
BbvI GCAGC 3 cut(s) 87, 317, 454
BccI CCATC 5 cut(s) 56, 248, 336, 362, 542
BcoDI GTCTC 1 cut(s) 134
BfaI CTAG 1 cut(s) 338
BfoI RGCGCY 1 cut(s) 193
BisI GCNGC 6 cut(s) 76, 206, 209, 306, 468, 517
BlpI GCTNAGC 1 cut(s) 18
BlsI GCNGC 6 cut(s) 77, 207, 210, 307, 469, 518
BmgT120I GGNCC 2 cut(s) 131, 132
BmiI GGNNCC 1 cut(s) 133
BmsI GCATC 2 cut(s) 238, 388
Bpu1102I GCTNAGC 1 cut(s) 18
BsaBI GATNNNNATC 1 cut(s) 438
BsaI GGTCTC 1 cut(s) 134
BsaJI CCNNGG 1 cut(s) 146
BsaWI WCCGGW 2 cut(s) 64, 216
Bsc4I CCNNNNNNNGG 3 cut(s) 68, 129, 152
Bse1I ACTGG 1 cut(s) 229
Bse21I CCTNAGG 1 cut(s) 462
Bse8I GATNNNNATC 1 cut(s) 438
BseAI TCCGGA 1 cut(s) 64
BseDI CCNNGG 1 cut(s) 146
BseGI GGATG 2 cut(s) 259, 403
BseJI GATNNNNATC 1 cut(s) 438
BseLI CCNNNNNNNGG 3 cut(s) 68, 129, 152
BseMII CTCAG 2 cut(s) 32, 453
BseNI ACTGG 1 cut(s) 229
BseSI GKGCMC 2 cut(s) 135, 376
BseXI GCAGC 3 cut(s) 87, 317, 454
Bsh1236I CGCG 2 cut(s) 119, 211
BshFI GGCC 1 cut(s) 133
BsiHKAI GWGCWC 2 cut(s) 376, 526
BsiSI CCGG 3 cut(s) 25, 65, 217
BslI CCNNNNNNNGG 3 cut(s) 68, 129, 152
BsmAI GTCTC 1 cut(s) 134
BsnI GGCC 1 cut(s) 133
Bso31I GGTCTC 1 cut(s) 134
Bsp120I GGGCCC 1 cut(s) 131
Bsp1286I GDGCHC 3 cut(s) 135, 376, 526
Bsp13I TCCGGA 1 cut(s) 64
Bsp143I GATC 3 cut(s) 5, 37, 433
Bsp1720I GCTNAGC 1 cut(s) 18
BspACI CCGC 4 cut(s) 206, 209, 251, 517
BspANI GGCC 1 cut(s) 133
BspCNI CTCAG 2 cut(s) 31, 454
BspEI TCCGGA 1 cut(s) 64
BspFNI CGCG 2 cut(s) 119, 211
BspLI GGNNCC 1 cut(s) 133
BspPI GGATC 2 cut(s) 13, 428
BspTNI GGTCTC 1 cut(s) 134
BsrI ACTGG 1 cut(s) 229
BssECI CCNNGG 1 cut(s) 146
BssMI GATC 3 cut(s) 5, 37, 433
BssSI CACGAG 1 cut(s) 525
BssT1I CCWWGG 1 cut(s) 146
Bst2BI CACGAG 1 cut(s) 525
Bst4CI ACNGT 1 cut(s) 378
Bst6I CTCTTC 1 cut(s) 110
BstDEI CTNAG 4 cut(s) 18, 301, 462, 543
BstEII GGTNACC 1 cut(s) 405
BstENI CCTNNNNNAGG 1 cut(s) 150
BstF5I GGATG 2 cut(s) 259, 403
BstFNI CGCG 2 cut(s) 119, 211
BstH2I RGCGCY 1 cut(s) 193
BstHHI GCGC 3 cut(s) 80, 119, 192
BstKTI GATC 3 cut(s) 8, 40, 436
BstMAI GTCTC 1 cut(s) 134
BstMBI GATC 3 cut(s) 5, 37, 433
BstPI GGTNACC 1 cut(s) 405
BstSLI GKGCMC 2 cut(s) 135, 376
BstUI CGCG 2 cut(s) 119, 211
BstV1I GCAGC 3 cut(s) 87, 317, 454
Bsu36I CCTNAGG 1 cut(s) 462
BsuRI GGCC 1 cut(s) 133
BtsCI GGATG 2 cut(s) 259, 403
BtsIMutI CAGTG 3 cut(s) 190, 374, 429
CfoI GCGC 3 cut(s) 80, 119, 192
Cfr13I GGNCC 2 cut(s) 131, 132
Csp6I GTAC 1 cut(s) 512
CviAII CATG 6 cut(s) 31, 365, 382, 402, 485, 490
CviJI RGCY 7 cut(s) 22, 28, 61, 133, 205, 467, 497
CviKI_1 RGCY 7 cut(s) 22, 28, 61, 133, 205, 467, 497
CviQI GTAC 1 cut(s) 512
DdeI CTNAG 4 cut(s) 18, 301, 462, 543
DpnI GATC 3 cut(s) 7, 39, 435
DpnII GATC 3 cut(s) 5, 37, 433
Eam1104I CTCTTC 1 cut(s) 110
EarI CTCTTC 1 cut(s) 110
Eco130I CCWWGG 1 cut(s) 146
Eco24I GRGCYC 1 cut(s) 135
Eco31I GGTCTC 1 cut(s) 134
Eco47III AGCGCT 1 cut(s) 191
Eco57I CTGAAG 1 cut(s) 166
Eco81I CCTNAGG 1 cut(s) 462
Eco91I GGTNACC 1 cut(s) 405
EcoNI CCTNNNNNAGG 1 cut(s) 150
EcoO65I GGTNACC 1 cut(s) 405
EcoT14I CCWWGG 1 cut(s) 146
EcoT22I ATGCAT 1 cut(s) 403
EcoT38I GRGCYC 1 cut(s) 135
ErhI CCWWGG 1 cut(s) 146
FaeI CATG 6 cut(s) 34, 368, 385, 405, 488, 493
FatI CATG 6 cut(s) 30, 364, 381, 401, 484, 489
FauNDI CATATG 1 cut(s) 310
Fnu4HI GCNGC 6 cut(s) 76, 206, 209, 306, 468, 517
FokI GGATG 2 cut(s) 266, 410
FriOI GRGCYC 1 cut(s) 135
Fsp4HI GCNGC 6 cut(s) 76, 206, 209, 306, 468, 517
FspBI CTAG 1 cut(s) 338
GlaI GCGC 3 cut(s) 79, 118, 191
GluI GCNGC 6 cut(s) 76, 206, 209, 306, 468, 517
HaeII RGCGCY 1 cut(s) 193
HaeIII GGCC 1 cut(s) 133
HapII CCGG 3 cut(s) 25, 65, 217
HhaI GCGC 3 cut(s) 80, 119, 192
Hin1II CATG 6 cut(s) 34, 368, 385, 405, 488, 493
Hin6I GCGC 3 cut(s) 78, 117, 190
HinP1I GCGC 3 cut(s) 78, 117, 190
HindIII AAGCTT 1 cut(s) 495
HinfI GANTC 3 cut(s) 324, 530, 539
HpaII CCGG 3 cut(s) 25, 65, 217
HphI GGTGA 2 cut(s) 280, 399
Hpy166II GTNNAC 3 cut(s) 222, 288, 374
Hpy188I TCNGA 3 cut(s) 37, 94, 321
Hpy188III TCNNGA 5 cut(s) 65, 197, 338, 395, 527
Hpy8I GTNNAC 3 cut(s) 222, 288, 374
HpyAV CCTTC 3 cut(s) 146, 190, 453
HpyCH4III ACNGT 1 cut(s) 378
HpyCH4IV ACGT 1 cut(s) 89
HpyCH4V TGCA 5 cut(s) 75, 374, 381, 401, 455
HpyF3I CTNAG 4 cut(s) 18, 301, 462, 543
HpySE526I ACGT 1 cut(s) 89
Hsp92II CATG 6 cut(s) 34, 368, 385, 405, 488, 493
HspAI GCGC 3 cut(s) 78, 117, 190
Kpn2I TCCGGA 1 cut(s) 64
Kzo9I GATC 3 cut(s) 5, 37, 433
LmnI GCTCC 2 cut(s) 27, 114
LpnPI CCDG 8 cut(s) 38, 78, 230, 242, 380, 424, 465, 474
Lsp1109I GCAGC 3 cut(s) 87, 317, 454
LweI GCATC 2 cut(s) 238, 388
MaeI CTAG 1 cut(s) 338
MaeII ACGT 1 cut(s) 89
MaeIII GTNAC 2 cut(s) 296, 405
MalI GATC 3 cut(s) 7, 39, 435
MboI GATC 3 cut(s) 5, 37, 433
MboII GAAGA 1 cut(s) 97
MhlI GDGCHC 3 cut(s) 135, 376, 526
MluCI AATT 3 cut(s) 242, 258, 390
MnlI CCTC 7 cut(s) 22, 61, 72, 113, 345, 457, 501
Mph1103I ATGCAT 1 cut(s) 403
MroI TCCGGA 1 cut(s) 64
MslI CAYNNNNRTG 1 cut(s) 369
MspA1I CMGCKG 1 cut(s) 519
MspI CCGG 3 cut(s) 25, 65, 217
MvnI CGCG 2 cut(s) 119, 211
NdeI CATATG 1 cut(s) 310
NdeII GATC 3 cut(s) 5, 37, 433
NlaIII CATG 6 cut(s) 34, 368, 385, 405, 488, 493
NlaIV GGNNCC 1 cut(s) 133
NmuCI GTSAC 1 cut(s) 405
NsiI ATGCAT 1 cut(s) 403
PfeI GAWTC 2 cut(s) 324, 530
PkrI GCNGC 6 cut(s) 77, 207, 210, 307, 469, 518
PspEI GGTNACC 1 cut(s) 405
PspN4I GGNNCC 1 cut(s) 133
PspOMI GGGCCC 1 cut(s) 131
PspPI GGNCC 2 cut(s) 131, 132
RsaI GTAC 1 cut(s) 513
RsaNI GTAC 1 cut(s) 512
RseI CAYNNNNRTG 1 cut(s) 369
SatI GCNGC 6 cut(s) 76, 206, 209, 306, 468, 517
Sau3AI GATC 3 cut(s) 5, 37, 433
Sau96I GGNCC 2 cut(s) 131, 132
SduI GDGCHC 3 cut(s) 135, 376, 526
SetI ASST 6 cut(s) 24, 92, 105, 157, 182, 499
SfaNI GCATC 2 cut(s) 238, 388
SmiMI CAYNNNNRTG 1 cut(s) 369
Sse9I AATT 3 cut(s) 242, 258, 390
SsiI CCGC 4 cut(s) 206, 209, 251, 517
SspMI CTAG 1 cut(s) 338
StyI CCWWGG 1 cut(s) 146
TaaI ACNGT 1 cut(s) 378
TaiI ACGT 1 cut(s) 92
TaqI TCGA 1 cut(s) 537
TasI AATT 3 cut(s) 242, 258, 390
TauI GCSGC 3 cut(s) 208, 211, 519
TfiI GAWTC 2 cut(s) 324, 530
TscAI CASTG 3 cut(s) 190, 381, 436
TseFI GTSAC 1 cut(s) 405
TseI GCWGC 3 cut(s) 75, 305, 467
Tsp45I GTSAC 1 cut(s) 405
TspDTI ATGAA 1 cut(s) 506
TspRI CASTG 3 cut(s) 190, 381, 436
VneI GTGCAC 1 cut(s) 372
XagI CCTNNNNNAGG 1 cut(s) 150
XapI RAATTY 1 cut(s) 242
XbaI TCTAGA 1 cut(s) 337
XspI CTAG 1 cut(s) 338
Zsp2I ATGCAT 1 cut(s) 403
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.