RchiOBHm_Chr2g0109261

Prokaryotic E2 family B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0014640
Physical Location & Seq
Reverse (-)
20682909 .. 20685903
2995 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ48310

Sequence Viewer

Length: 873 bp
ATGAATTTTTTTACCGCCAAAACCGCAAGCATTTACCCACCAAAACCCAAACCAGGGGGCTCAAAACACAAAGAGCCCACAGCCAAGGTTGACTCCACCAGCAAACAAATCTGGAAAAGGAATAGAATTTTACAGGTAGCAAAAGAAACACAGAGACAAACAGAGTCGGGTTTCCATTCCTTCCTTTCTTCTTTGTCAGATTTCAGCCAAAACGGGTTTCAGATTCCAAACCCCTTTCGTTCTCATCCACCACGACCACCACTCACCCATTCCCACCTTTTAAACTATAAACCAAAAAGAAAAAGCCAAAATCCAAATCCGCGTCTTCTTCGCTCTTTGGCTTTATTATATATACTCCGCCAGGTTGGTTTTGGGTTTCCAGAACAACAAGATCAACAACTCATCGAGCCTCTCTCTGCTATTATGGGATCGTCCTCTGCTCAGCTCCGGCTCATGTCCGATCTCAAAACCATCATAAACGAGCCTCCGGAGGGTTGCAGCGCAAGTCCTACGTCCGATGATAACCTGTTCGTGTGGAGCGCGACCATATTTGGGCCCGATGAGACCCCTTGGGAAGGTGGTGTTTTCAGTTTGCGATTGACCTTCAGTGAGCGCTATCCCGAAAAGCCGCCGCGTGTCCGGTTCACGTCCGAGATGTTCCATCCAAATGTTTACCATGATGGTGCACTGTGCATGGATATAATTCAGGATGCATGGTCACCCTGCCACAACGTATCAACAATTTTAACATCTGTTCAATCACTTCTCACTGATCCAAATCCTTCAAGTCCTGCAAACCCTGAGGCTGCTCAAATGTATCAGCATGACATAAAAGCTTACAACAAGTGTGAGGGTACGCCGCTGTGCTCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

290

Amino Acids

32.57

Weight (kDa)

8.75

Isoelectric Point (pI)

54.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 150 - 282 2e-48 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014640)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18560 FvH4_1g18560 FvH4_1g18560
malus_domestica MD02G1193300.v1.1 MD15G1303600.v1.1
prunus_persica Prupe.6G213500_v2.0.a1
pyrus_communis pycom02g15700 pycom15g26650
rosa_chinensis RchiOBHm_Chr2g0109261
rosa_laevigata RLG00000017729
rosa_multiflora Rmu_sc0001807.1_g000008
rosa_roxburghii Rroxscaffold_2G00134350
rosa_rugosa Rorug02G0158900
rosa_samantha Rh2AG210300 Rh2BG220700 Rh2CG212700 Rh2DG216500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 322, 542, 634
AccIII TCCGGA 1 cut(s) 487
AciI CCGC 7 cut(s) 15, 24, 320, 358, 629, 632, 860
AclWI GGATC 2 cut(s) 436, 767
AcsI RAATTY 2 cut(s) 4, 126
AcuI CTGAAG 1 cut(s) 589
AfaI GTAC 1 cut(s) 856
AfeI AGCGCT 1 cut(s) 614
AfiI CCNNNNNNNGG 5 cut(s) 53, 54, 491, 552, 575
AgsI TTSAA 2 cut(s) 758, 786
AjiI CACGTC 1 cut(s) 648
AjnI CCWGG 2 cut(s) 52, 360
AjuI GAANNNNNNNTTGG 2 cut(s) 201, 233
AluBI AGCT 2 cut(s) 445, 836
AluI AGCT 2 cut(s) 445, 836
Alw21I GWGCWC 2 cut(s) 688, 869
Alw26I GTCTC 2 cut(s) 148, 557
Alw44I GTGCAC 1 cut(s) 684
AlwI GGATC 2 cut(s) 436, 767
Aor13HI TCCGGA 1 cut(s) 487
Aor51HI AGCGCT 1 cut(s) 614
AoxI GGCC 1 cut(s) 554
ApaI GGGCCC 1 cut(s) 558
ApaLI GTGCAC 1 cut(s) 684
ApeKI GCWGC 2 cut(s) 498, 806
ApoI RAATTY 2 cut(s) 4, 126
AspLEI GCGC 3 cut(s) 503, 542, 615
AspS9I GGNCC 2 cut(s) 554, 555
AsuHPI GGTGA 2 cut(s) 256, 711
AxyI CCTNAGG 1 cut(s) 801
BaeGI GKGCMC 2 cut(s) 558, 688
BanII GRGCYC 3 cut(s) 62, 78, 558
BauI CACGAG 1 cut(s) 868
BbsI GAAGAC 1 cut(s) 317
Bbv12I GWGCWC 2 cut(s) 688, 869
BbvI GCAGC 2 cut(s) 510, 793
BccI CCATC 3 cut(s) 479, 669, 674
BciT130I CCWGG 2 cut(s) 54, 362
BcoDI GTCTC 2 cut(s) 148, 557
BfoI RGCGCY 1 cut(s) 616
BisI GCNGC 5 cut(s) 499, 629, 632, 807, 860
BlpI GCTNAGC 1 cut(s) 441
BlsI GCNGC 5 cut(s) 500, 630, 633, 808, 861
Bme1390I CCNGG 2 cut(s) 54, 362
BmgBI CACGTC 1 cut(s) 648
BmgT120I GGNCC 2 cut(s) 554, 555
BmiI GGNNCC 1 cut(s) 556
BmrFI CCNGG 2 cut(s) 54, 362
BmsI GCATC 1 cut(s) 700
BpiI GAAGAC 1 cut(s) 317
BplI GAGNNNNNCTC 2 cut(s) 398, 430
Bpu1102I GCTNAGC 1 cut(s) 441
BsaBI GATNNNNATC 1 cut(s) 777
BsaI GGTCTC 1 cut(s) 557
BsaJI CCNNGG 3 cut(s) 53, 84, 569
BsaWI WCCGGW 2 cut(s) 487, 639
Bsc4I CCNNNNNNNGG 5 cut(s) 53, 54, 491, 552, 575
Bse21I CCTNAGG 1 cut(s) 801
Bse8I GATNNNNATC 1 cut(s) 777
BseAI TCCGGA 1 cut(s) 487
BseBI CCWGG 2 cut(s) 54, 362
BseDI CCNNGG 3 cut(s) 53, 84, 569
BseGI GGATG 3 cut(s) 244, 661, 715
BseJI GATNNNNATC 1 cut(s) 777
BseLI CCNNNNNNNGG 5 cut(s) 53, 54, 491, 552, 575
BseMII CTCAG 2 cut(s) 455, 792
BseSI GKGCMC 2 cut(s) 558, 688
BseXI GCAGC 2 cut(s) 510, 793
Bsh1236I CGCG 3 cut(s) 322, 542, 634
BshFI GGCC 1 cut(s) 556
BsiHKAI GWGCWC 2 cut(s) 688, 869
BsiSI CCGG 3 cut(s) 448, 488, 640
BslI CCNNNNNNNGG 5 cut(s) 53, 54, 491, 552, 575
BsmAI GTCTC 2 cut(s) 148, 557
BsnI GGCC 1 cut(s) 556
Bso31I GGTCTC 1 cut(s) 557
Bsp120I GGGCCC 1 cut(s) 554
Bsp1286I GDGCHC 5 cut(s) 62, 78, 558, 688, 869
Bsp13I TCCGGA 1 cut(s) 487
Bsp143I GATC 4 cut(s) 391, 428, 460, 772
Bsp1720I GCTNAGC 1 cut(s) 441
BspACI CCGC 7 cut(s) 15, 24, 320, 358, 629, 632, 860
BspANI GGCC 1 cut(s) 556
BspCNI CTCAG 2 cut(s) 454, 793
BspEI TCCGGA 1 cut(s) 487
BspFNI CGCG 3 cut(s) 322, 542, 634
BspLI GGNNCC 1 cut(s) 556
BspPI GGATC 2 cut(s) 436, 767
BspTNI GGTCTC 1 cut(s) 557
BssECI CCNNGG 3 cut(s) 53, 84, 569
BssMI GATC 4 cut(s) 391, 428, 460, 772
BssSI CACGAG 1 cut(s) 868
BssT1I CCWWGG 2 cut(s) 84, 569
Bst2BI CACGAG 1 cut(s) 868
Bst2UI CCWGG 2 cut(s) 54, 362
Bst4CI ACNGT 1 cut(s) 690
BstC8I GCNNGC 1 cut(s) 28
BstDEI CTNAG 2 cut(s) 441, 801
BstEII GGTNACC 1 cut(s) 717
BstENI CCTNNNNNAGG 1 cut(s) 573
BstF5I GGATG 3 cut(s) 244, 661, 715
BstFNI CGCG 3 cut(s) 322, 542, 634
BstH2I RGCGCY 1 cut(s) 616
BstHHI GCGC 3 cut(s) 503, 542, 615
BstKTI GATC 4 cut(s) 394, 431, 463, 775
BstMAI GTCTC 2 cut(s) 148, 557
BstMBI GATC 4 cut(s) 391, 428, 460, 772
BstMWI GCNNNNNNNGC 1 cut(s) 23
BstNI CCWGG 2 cut(s) 54, 362
BstPI GGTNACC 1 cut(s) 717
BstSCI CCNGG 2 cut(s) 52, 360
BstSLI GKGCMC 2 cut(s) 558, 688
BstUI CGCG 3 cut(s) 322, 542, 634
BstV1I GCAGC 2 cut(s) 510, 793
BstV2I GAAGAC 1 cut(s) 317
Bsu36I CCTNAGG 1 cut(s) 801
BsuRI GGCC 1 cut(s) 556
BtrI CACGTC 1 cut(s) 648
BtsCI GGATG 3 cut(s) 244, 661, 715
BtsIMutI CAGTG 3 cut(s) 613, 686, 768
Cac8I GCNNGC 1 cut(s) 28
CfoI GCGC 3 cut(s) 503, 542, 615
Cfr13I GGNCC 2 cut(s) 554, 555
CseI GACGC 1 cut(s) 311
Csp6I GTAC 1 cut(s) 855
CviAII CATG 5 cut(s) 454, 677, 694, 714, 824
CviQI GTAC 1 cut(s) 855
DdeI CTNAG 2 cut(s) 441, 801
DpnI GATC 4 cut(s) 393, 430, 462, 774
DpnII GATC 4 cut(s) 391, 428, 460, 772
DraI TTTAAA 1 cut(s) 282
EciI GGCGGA 1 cut(s) 347
Eco130I CCWWGG 2 cut(s) 84, 569
Eco24I GRGCYC 3 cut(s) 62, 78, 558
Eco31I GGTCTC 1 cut(s) 557
Eco47III AGCGCT 1 cut(s) 614
Eco57I CTGAAG 1 cut(s) 589
Eco81I CCTNAGG 1 cut(s) 801
Eco91I GGTNACC 1 cut(s) 717
EcoNI CCTNNNNNAGG 1 cut(s) 573
EcoO65I GGTNACC 1 cut(s) 717
EcoRII CCWGG 2 cut(s) 52, 360
EcoT14I CCWWGG 2 cut(s) 84, 569
EcoT22I ATGCAT 1 cut(s) 715
EcoT38I GRGCYC 3 cut(s) 62, 78, 558
ErhI CCWWGG 2 cut(s) 84, 569
FaeI CATG 5 cut(s) 457, 680, 697, 717, 827
FatI CATG 5 cut(s) 453, 676, 693, 713, 823
Fnu4HI GCNGC 5 cut(s) 499, 629, 632, 807, 860
FokI GGATG 3 cut(s) 231, 648, 722
FriOI GRGCYC 3 cut(s) 62, 78, 558
Fsp4HI GCNGC 5 cut(s) 499, 629, 632, 807, 860
GlaI GCGC 3 cut(s) 502, 541, 614
GluI GCNGC 5 cut(s) 499, 629, 632, 807, 860
HaeII RGCGCY 1 cut(s) 616
HaeIII GGCC 1 cut(s) 556
HapII CCGG 3 cut(s) 448, 488, 640
HgaI GACGC 1 cut(s) 311
HhaI GCGC 3 cut(s) 503, 542, 615
Hin1II CATG 5 cut(s) 457, 680, 697, 717, 827
Hin6I GCGC 3 cut(s) 501, 540, 613
HinP1I GCGC 3 cut(s) 501, 540, 613
HincII GTYRAC 1 cut(s) 91
HindII GTYRAC 1 cut(s) 91
HindIII AAGCTT 1 cut(s) 834
HinfI GANTC 3 cut(s) 92, 164, 223
HpaII CCGG 3 cut(s) 448, 488, 640
HphI GGTGA 2 cut(s) 256, 711
Hpy166II GTNNAC 4 cut(s) 91, 645, 673, 686
Hpy188I TCNGA 5 cut(s) 199, 222, 460, 517, 652
Hpy188III TCNNGA 6 cut(s) 112, 380, 488, 620, 707, 870
Hpy8I GTNNAC 4 cut(s) 91, 645, 673, 686
HpyAV CCTTC 4 cut(s) 190, 569, 613, 792
HpyCH4III ACNGT 1 cut(s) 690
HpyCH4IV ACGT 3 cut(s) 512, 647, 732
HpyCH4V TGCA 5 cut(s) 498, 686, 693, 713, 794
HpyF10VI GCNNNNNNNGC 1 cut(s) 23
HpyF3I CTNAG 2 cut(s) 441, 801
HpySE526I ACGT 3 cut(s) 512, 647, 732
Hsp92II CATG 5 cut(s) 457, 680, 697, 717, 827
HspAI GCGC 3 cut(s) 501, 540, 613
Kpn2I TCCGGA 1 cut(s) 487
Kzo9I GATC 4 cut(s) 391, 428, 460, 772
LmnI GCTCC 2 cut(s) 450, 537
Lsp1109I GCAGC 2 cut(s) 510, 793
LweI GCATC 1 cut(s) 700
MaeII ACGT 3 cut(s) 512, 647, 732
MaeIII GTNAC 1 cut(s) 717
MalI GATC 4 cut(s) 393, 430, 462, 774
MboI GATC 4 cut(s) 391, 428, 460, 772
MboII GAAGA 3 cut(s) 180, 317, 320
MhlI GDGCHC 5 cut(s) 62, 78, 558, 688, 869
MluCI AATT 4 cut(s) 4, 126, 702, 741
MlyI GAGTC 2 cut(s) 86, 173
MnlI CCTC 6 cut(s) 420, 445, 484, 495, 796, 844
Mph1103I ATGCAT 1 cut(s) 715
MroI TCCGGA 1 cut(s) 487
MseI TTAA 2 cut(s) 281, 746
MslI CAYNNNNRTG 2 cut(s) 666, 681
MspA1I CMGCKG 1 cut(s) 862
MspI CCGG 3 cut(s) 448, 488, 640
MspR9I CCNGG 2 cut(s) 54, 362
MvaI CCWGG 2 cut(s) 54, 362
MvnI CGCG 3 cut(s) 322, 542, 634
MwoI GCNNNNNNNGC 1 cut(s) 23
NdeII GATC 4 cut(s) 391, 428, 460, 772
NlaIII CATG 5 cut(s) 457, 680, 697, 717, 827
NlaIV GGNNCC 1 cut(s) 556
NmuCI GTSAC 1 cut(s) 717
NsiI ATGCAT 1 cut(s) 715
PfeI GAWTC 1 cut(s) 223
PkrI GCNGC 5 cut(s) 500, 630, 633, 808, 861
PleI GAGTC 2 cut(s) 86, 172
PpsI GAGTC 2 cut(s) 86, 172
Psp6I CCWGG 2 cut(s) 52, 360
PspEI GGTNACC 1 cut(s) 717
PspGI CCWGG 2 cut(s) 52, 360
PspN4I GGNNCC 1 cut(s) 556
PspOMI GGGCCC 1 cut(s) 554
PspPI GGNCC 2 cut(s) 554, 555
RsaI GTAC 1 cut(s) 856
RsaNI GTAC 1 cut(s) 855
RseI CAYNNNNRTG 2 cut(s) 666, 681
SaqAI TTAA 2 cut(s) 281, 746
SatI GCNGC 5 cut(s) 499, 629, 632, 807, 860
Sau3AI GATC 4 cut(s) 391, 428, 460, 772
Sau96I GGNCC 2 cut(s) 554, 555
SchI GAGTC 2 cut(s) 86, 173
ScrFI CCNGG 2 cut(s) 54, 362
SduI GDGCHC 5 cut(s) 62, 78, 558, 688, 869
SfaNI GCATC 1 cut(s) 700
SmiMI CAYNNNNRTG 2 cut(s) 666, 681
Sse9I AATT 4 cut(s) 4, 126, 702, 741
SsiI CCGC 7 cut(s) 15, 24, 320, 358, 629, 632, 860
StyD4I CCNGG 2 cut(s) 52, 360
StyI CCWWGG 2 cut(s) 84, 569
TaaI ACNGT 1 cut(s) 690
TaiI ACGT 3 cut(s) 515, 650, 735
TaqI TCGA 1 cut(s) 405
TasI AATT 4 cut(s) 4, 126, 702, 741
TauI GCSGC 3 cut(s) 631, 634, 862
TfiI GAWTC 1 cut(s) 223
Tru1I TTAA 2 cut(s) 281, 746
Tru9I TTAA 2 cut(s) 281, 746
TscAI CASTG 3 cut(s) 613, 693, 775
TseFI GTSAC 1 cut(s) 717
TseI GCWGC 2 cut(s) 498, 806
Tsp45I GTSAC 1 cut(s) 717
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 3 cut(s) 613, 693, 775
VneI GTGCAC 1 cut(s) 684
XagI CCTNNNNNAGG 1 cut(s) 573
XapI RAATTY 2 cut(s) 4, 126
XcmI CCANNNNNNNNNTGG 1 cut(s) 368
Zsp2I ATGCAT 1 cut(s) 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.