MD02G1193300.v1.1

Prokaryotic E2 family B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014640
Physical Location & Seq
Reverse (-)
18373463 .. 18375845
2383 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1193300.v1.1.491

Sequence Viewer

Length: 468 bp
ATGGCGTCGTCGGCGCAACTCCGGCTCATGTCCGATCTCAGAACCATCATCAACGAGCCCCCCGAGGGTTGCAGCGCCAGCCCTCTGTCTGATGACAATCTGATGGTGTGGAGCGCCACCATATTCGGGCCTGATGAGACCCCATGGGAAGGTTGTGGTGTTTTTAGCTTGAGGTTGACATTTAGTGAACGTTATCCCGAAAAGCCGCCTCGGGTTCGTTTCACCTCCGAAATTTTCCATCCAAATGTTTATCGTGATGGTTCACTGTGCATGGATATAATTCAGGATGCATGGTCACCATGCCACAATGTATCTACGATTTTGACGTCTGTTCAATCACTTCTCACTGATCCAAACGCTGCAAGTCCAGCAAACCCTGATGCTGCTCACATGTATCAGCATGATATACAAGCTTACAACAAGAGGGTACGCCGTTGTGCTCGTCATTCCATTGAGTCTTTGCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04120 map04120
Pfam Domains
Protein Families
UQ_con

Protein Analysis

156

Amino Acids

17.4

Weight (kDa)

5.44

Isoelectric Point (pI)

61.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
UQ_con PF00179 8 - 146 1.1e-47 Ubiquitin-conjugating enzyme
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014640)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18560 FvH4_1g18560 FvH4_1g18560
malus_domestica MD02G1193300.v1.1 MD15G1303600.v1.1
prunus_persica Prupe.6G213500_v2.0.a1
pyrus_communis pycom02g15700 pycom15g26650
rosa_chinensis RchiOBHm_Chr2g0109261
rosa_laevigata RLG00000017729
rosa_multiflora Rmu_sc0001807.1_g000008
rosa_roxburghii Rroxscaffold_2G00134350
rosa_rugosa Rorug02G0158900
rosa_samantha Rh2AG210300 Rh2BG220700 Rh2CG212700 Rh2DG216500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 329
AciI CCGC 1 cut(s) 206
AclI AACGTT 1 cut(s) 190
AclWI GGATC 1 cut(s) 344
AcsI RAATTY 1 cut(s) 231
AcyI GRCGYC 2 cut(s) 5, 326
AfaI GTAC 1 cut(s) 429
AfiI CCNNNNNNNGG 3 cut(s) 65, 126, 149
AflIII ACRYGT 1 cut(s) 390
AgsI TTSAA 1 cut(s) 335
AluBI AGCT 2 cut(s) 168, 413
AluI AGCT 2 cut(s) 168, 413
Alw21I GWGCWC 1 cut(s) 442
Alw26I GTCTC 1 cut(s) 131
AlwI GGATC 1 cut(s) 344
Ama87I CYCGRG 2 cut(s) 62, 210
AoxI GGCC 1 cut(s) 128
ApeKI GCWGC 3 cut(s) 72, 359, 383
ApoI RAATTY 1 cut(s) 231
AspLEI GCGC 3 cut(s) 16, 77, 116
AspS9I GGNCC 1 cut(s) 128
AsuHPI GGTGA 2 cut(s) 214, 288
AvaI CYCGRG 2 cut(s) 62, 210
BanII GRGCYC 1 cut(s) 60
Bbv12I GWGCWC 1 cut(s) 442
BbvI GCAGC 3 cut(s) 84, 346, 370
BccI CCATC 4 cut(s) 53, 97, 246, 251
BceAI ACGGC 1 cut(s) 417
BcoDI GTCTC 1 cut(s) 131
BfoI RGCGCY 2 cut(s) 78, 117
BisI GCNGC 4 cut(s) 73, 206, 360, 384
BlsI GCNGC 4 cut(s) 74, 207, 361, 385
BmeT110I CYCGRG 2 cut(s) 62, 210
BmgT120I GGNCC 1 cut(s) 128
BmsI GCATC 2 cut(s) 277, 370
BpuEI CTTGAG 1 cut(s) 190
BsaBI GATNNNNATC 1 cut(s) 96
BsaHI GRCGYC 2 cut(s) 5, 326
BsaI GGTCTC 1 cut(s) 131
BsaJI CCNNGG 3 cut(s) 63, 143, 209
Bsc4I CCNNNNNNNGG 3 cut(s) 65, 126, 149
Bse8I GATNNNNATC 1 cut(s) 96
BseDI CCNNGG 3 cut(s) 63, 143, 209
BseGI GGATG 2 cut(s) 238, 292
BseJI GATNNNNATC 1 cut(s) 96
BseLI CCNNNNNNNGG 3 cut(s) 65, 126, 149
BseMII CTCAG 1 cut(s) 52
BseXI GCAGC 3 cut(s) 84, 346, 370
BshFI GGCC 1 cut(s) 130
BsiHKAI GWGCWC 1 cut(s) 442
BsiHKCI CYCGRG 2 cut(s) 62, 210
BsiSI CCGG 1 cut(s) 22
BslI CCNNNNNNNGG 3 cut(s) 65, 126, 149
BsmAI GTCTC 1 cut(s) 131
BsnI GGCC 1 cut(s) 130
Bso31I GGTCTC 1 cut(s) 131
BsoBI CYCGRG 2 cut(s) 62, 210
Bsp1286I GDGCHC 2 cut(s) 60, 442
Bsp143I GATC 2 cut(s) 34, 349
Bsp19I CCATGG 1 cut(s) 143
BspACI CCGC 1 cut(s) 206
BspANI GGCC 1 cut(s) 130
BspCNI CTCAG 1 cut(s) 51
BspPI GGATC 1 cut(s) 344
BspTNI GGTCTC 1 cut(s) 131
BssECI CCNNGG 3 cut(s) 63, 143, 209
BssMI GATC 2 cut(s) 34, 349
BssNI GRCGYC 2 cut(s) 5, 326
BssT1I CCWWGG 1 cut(s) 143
Bst4CI ACNGT 1 cut(s) 267
BstACI GRCGYC 2 cut(s) 5, 326
BstC8I GCNNGC 1 cut(s) 79
BstDEI CTNAG 1 cut(s) 38
BstDSI CCRYGG 1 cut(s) 143
BstEII GGTNACC 1 cut(s) 294
BstF5I GGATG 2 cut(s) 238, 292
BstH2I RGCGCY 2 cut(s) 78, 117
BstHHI GCGC 3 cut(s) 16, 77, 116
BstKTI GATC 2 cut(s) 37, 352
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 2 cut(s) 34, 349
BstMWI GCNNNNNNNGC 4 cut(s) 11, 22, 78, 368
BstNSI RCATGY 1 cut(s) 394
BstPI GGTNACC 1 cut(s) 294
BstV1I GCAGC 3 cut(s) 84, 346, 370
BsuRI GGCC 1 cut(s) 130
BtgI CCRYGG 1 cut(s) 143
BtsCI GGATG 2 cut(s) 238, 292
BtsIMutI CAGTG 2 cut(s) 263, 345
Cac8I GCNNGC 1 cut(s) 79
CfoI GCGC 3 cut(s) 16, 77, 116
Cfr13I GGNCC 1 cut(s) 128
Csp6I GTAC 1 cut(s) 428
CviAII CATG 7 cut(s) 28, 144, 271, 291, 300, 391, 401
CviJI RGCY 7 cut(s) 25, 58, 81, 130, 168, 205, 413
CviKI_1 RGCY 7 cut(s) 25, 58, 81, 130, 168, 205, 413
CviQI GTAC 1 cut(s) 428
DdeI CTNAG 1 cut(s) 38
DpnI GATC 2 cut(s) 36, 351
DpnII GATC 2 cut(s) 34, 349
Eco130I CCWWGG 1 cut(s) 143
Eco24I GRGCYC 1 cut(s) 60
Eco31I GGTCTC 1 cut(s) 131
Eco88I CYCGRG 2 cut(s) 62, 210
Eco91I GGTNACC 1 cut(s) 294
EcoO65I GGTNACC 1 cut(s) 294
EcoT14I CCWWGG 1 cut(s) 143
EcoT22I ATGCAT 1 cut(s) 292
EcoT38I GRGCYC 1 cut(s) 60
ErhI CCWWGG 1 cut(s) 143
FaeI CATG 7 cut(s) 31, 147, 274, 294, 303, 394, 404
FatI CATG 7 cut(s) 27, 143, 270, 290, 299, 390, 400
Fnu4HI GCNGC 4 cut(s) 73, 206, 360, 384
FokI GGATG 2 cut(s) 225, 299
FriOI GRGCYC 1 cut(s) 60
Fsp4HI GCNGC 4 cut(s) 73, 206, 360, 384
GlaI GCGC 3 cut(s) 15, 76, 115
GluI GCNGC 4 cut(s) 73, 206, 360, 384
HaeII RGCGCY 2 cut(s) 78, 117
HaeIII GGCC 1 cut(s) 130
HapII CCGG 1 cut(s) 22
HhaI GCGC 3 cut(s) 16, 77, 116
Hin1I GRCGYC 2 cut(s) 5, 326
Hin1II CATG 7 cut(s) 31, 147, 274, 294, 303, 394, 404
Hin6I GCGC 3 cut(s) 14, 75, 114
HinP1I GCGC 3 cut(s) 14, 75, 114
HincII GTYRAC 1 cut(s) 177
HindII GTYRAC 1 cut(s) 177
HindIII AAGCTT 1 cut(s) 411
HinfI GANTC 1 cut(s) 455
HpaII CCGG 1 cut(s) 22
HphI GGTGA 2 cut(s) 214, 288
Hpy166II GTNNAC 3 cut(s) 177, 188, 263
Hpy188I TCNGA 5 cut(s) 34, 41, 91, 102, 229
Hpy188III TCNNGA 3 cut(s) 197, 254, 284
Hpy8I GTNNAC 3 cut(s) 177, 188, 263
Hpy99I CGWCG 2 cut(s) 10, 13
HpyAV CCTTC 1 cut(s) 143
HpyCH4III ACNGT 1 cut(s) 267
HpyCH4IV ACGT 2 cut(s) 190, 326
HpyCH4V TGCA 5 cut(s) 72, 270, 290, 362, 463
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 22, 78, 368
HpyF3I CTNAG 1 cut(s) 38
HpySE526I ACGT 2 cut(s) 190, 326
Hsp92I GRCGYC 2 cut(s) 5, 326
Hsp92II CATG 7 cut(s) 31, 147, 274, 294, 303, 394, 404
HspAI GCGC 3 cut(s) 14, 75, 114
Kzo9I GATC 2 cut(s) 34, 349
LmnI GCTCC 1 cut(s) 111
LpnPI CCDG 6 cut(s) 35, 91, 144, 269, 381, 390
Lsp1109I GCAGC 3 cut(s) 84, 346, 370
LweI GCATC 2 cut(s) 277, 370
MaeII ACGT 2 cut(s) 190, 326
MaeIII GTNAC 1 cut(s) 294
MalI GATC 2 cut(s) 36, 351
MboI GATC 2 cut(s) 34, 349
MhlI GDGCHC 2 cut(s) 60, 442
MluCI AATT 2 cut(s) 231, 279
MlyI GAGTC 1 cut(s) 464
MnlI CCTC 6 cut(s) 58, 93, 165, 219, 235, 417
Mph1103I ATGCAT 1 cut(s) 292
MslI CAYNNNNRTG 1 cut(s) 243
MspI CCGG 1 cut(s) 22
MwoI GCNNNNNNNGC 4 cut(s) 11, 22, 78, 368
NcoI CCATGG 1 cut(s) 143
NdeII GATC 2 cut(s) 34, 349
NlaIII CATG 7 cut(s) 31, 147, 274, 294, 303, 394, 404
NmuCI GTSAC 1 cut(s) 294
NsiI ATGCAT 1 cut(s) 292
NspI RCATGY 1 cut(s) 394
PciI ACATGT 1 cut(s) 390
PcsI WCGNNNNNNNCGW 2 cut(s) 60, 323
PkrI GCNGC 4 cut(s) 74, 207, 361, 385
PleI GAGTC 1 cut(s) 463
PpsI GAGTC 1 cut(s) 463
PscI ACATGT 1 cut(s) 390
Psp1406I AACGTT 1 cut(s) 190
PspEI GGTNACC 1 cut(s) 294
PspPI GGNCC 1 cut(s) 128
RsaI GTAC 1 cut(s) 429
RsaNI GTAC 1 cut(s) 428
RseI CAYNNNNRTG 1 cut(s) 243
SatI GCNGC 4 cut(s) 73, 206, 360, 384
Sau3AI GATC 2 cut(s) 34, 349
Sau96I GGNCC 1 cut(s) 128
SchI GAGTC 1 cut(s) 464
SduI GDGCHC 2 cut(s) 60, 442
SetI ASST 7 cut(s) 154, 170, 176, 193, 227, 329, 415
SfaNI GCATC 2 cut(s) 277, 370
SmiMI CAYNNNNRTG 1 cut(s) 243
SmlI CTYRAG 1 cut(s) 169
SmoI CTYRAG 1 cut(s) 169
Sse9I AATT 2 cut(s) 231, 279
SsiI CCGC 1 cut(s) 206
StyI CCWWGG 1 cut(s) 143
TaaI ACNGT 1 cut(s) 267
TaiI ACGT 2 cut(s) 193, 329
TasI AATT 2 cut(s) 231, 279
TauI GCSGC 1 cut(s) 208
TscAI CASTG 2 cut(s) 270, 352
TseFI GTSAC 1 cut(s) 294
TseI GCWGC 3 cut(s) 72, 359, 383
Tsp45I GTSAC 1 cut(s) 294
TspRI CASTG 2 cut(s) 270, 352
XapI RAATTY 1 cut(s) 231
XceI RCATGY 1 cut(s) 394
ZraI GACGTC 1 cut(s) 327
Zsp2I ATGCAT 1 cut(s) 292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.