FvH4_1g20600

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0014642
Physical Location & Seq
Forward (+)
12788217 .. 12790107
1891 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g20600.t1

Sequence Viewer

Length: 954 bp
ATGGAACCACAACTAGCAGCATTAGCTCTTTCACTCATCATCTTCAACCTCCTCCTCACAACCACTACCCAAAACCCTTCCTGCCAAGCCACCTGCGGCTCCCTCCACCTCAGCTACCCTTTCGGCTCCGGCCCTGGTTGCGGCTCCCCAACCTTCCAACCATACACAACCTGCACAACCACCACCAAGCAGCTCCTTCTGACCACCCACACAGGGTCGTACCCCATCACCTCCATCTCCTACTCCACCAACACCCTGACCCTAACACCATCCTCCATGTCCACCTGCACCTCCATGCAACCCTCCCCCTCTAACCTGGGCCTGGACTGGGCCAGCCCGTTCCAGCTCGGCCCATCAACTTTCATCCTCCTCTCCTGCCAACCTCCCACCTCCTCTCTCACCCTCAAAGACTCCTCCATCCCAATCTGCGACCCCTCCTACTCCCACCTCTGCGCCGCCATCTACACATGTCCCTCCGTCACCGGCCTCAACCTCCCTCTCTTCCCTCCCACCAACACTTGCTGTGTCTACTCTCCCGGAAACCTAGACGCCAAGGGCGAGCTAGACCTCCGGGGGCTCAAGTGTAAGGGATACACCTCCGTCGTGTCGCTCGGGGACTACCCGACCGACCCGAACAAGTGGGAGTACGGGGTGGCGCTGAAGTATAACCACGGAGGCGGTTTGGATGGTGGCATTGTGGAGACGAAGTGCAAGAGTTGTGAGATGAGTGGTGGCGTTTGTGGTTATGCTACTTCTGATAACTCGTTTCTTTGTGTTTGTGAAAATGGCTACTACAACACCACTTCGGATTGTTCTTCTAATAATTACAGTGGTGTTCAAGAACTAGGTGACATCTGGAATTCGGAATCTGGCTCTGGTTTACCAGCTTCTTCAGCTTGGAAAACATTGTTTGGGCTCTTGGCGTTGGCTGACCTGATCATTTTAGCAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

318

Amino Acids

33.34

Weight (kDa)

4.69

Isoelectric Point (pI)

39.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 28 - 88 1.4e-10 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 234 - 262 9.7e-07 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014642)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20600
malus_domestica MD01G1032500.v1.1 MD15G1322500.v1.1
prunus_persica Prupe.6G189700_v2.0.a1
pyrus_communis pycom01g05980 pycom15g28310
rosa_chinensis RchiOBHm_Chr2g0113031
rosa_laevigata RLG00000018031
rosa_multiflora Rmu_sc0002351.1_g000105 Rmu_sc0024545.1_g000002
rosa_roxburghii Rroxscaffold_2G00130930
rosa_rugosa Rorug02G0183300
rosa_samantha Rh2AG238500 Rh2BG252300 Rh2CG242500 Rh2DG246500
rosa_wichuraiana Rw2G018700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 101, 293
Acc36I ACCTGC 3 cut(s) 101, 179, 293
AccI GTMKAC 1 cut(s) 528
AciI CCGC 4 cut(s) 96, 141, 456, 678
AcsI RAATTY 1 cut(s) 859
AcuI CTGAAG 2 cut(s) 680, 876
AcyI GRCGYC 1 cut(s) 549
AfaI GTAC 2 cut(s) 221, 647
AfiI CCNNNNNNNGG 4 cut(s) 140, 213, 322, 328
AflIII ACRYGT 1 cut(s) 467
AgsI TTSAA 2 cut(s) 46, 839
AjnI CCWGG 3 cut(s) 133, 315, 321
AluBI AGCT 8 cut(s) 26, 114, 193, 346, 562, 887, 896, 950
AluI AGCT 8 cut(s) 26, 114, 193, 346, 562, 887, 896, 950
Alw26I GTCTC 1 cut(s) 695
Ama87I CYCGRG 1 cut(s) 611
AoxI GGCC 5 cut(s) 130, 319, 330, 349, 484
ApeKI GCWGC 3 cut(s) 17, 190, 947
ApoI RAATTY 1 cut(s) 859
AspLEI GCGC 2 cut(s) 455, 658
AspS9I GGNCC 4 cut(s) 131, 319, 330, 350
AsuC2I CCSGG 2 cut(s) 537, 572
AsuHPI GGTGA 4 cut(s) 220, 391, 472, 860
AvaI CYCGRG 1 cut(s) 611
BanII GRGCYC 2 cut(s) 579, 918
BbvCI CCTCAGC 1 cut(s) 110
BbvI GCAGC 2 cut(s) 29, 202
BccI CCATC 7 cut(s) 233, 242, 277, 361, 425, 467, 680
BciT130I CCWGG 3 cut(s) 135, 317, 323
BciVI GTATCC 1 cut(s) 584
BclI TGATCA 1 cut(s) 936
BcnI CCSGG 2 cut(s) 537, 572
BcoDI GTCTC 1 cut(s) 695
BfaI CTAG 4 cut(s) 14, 545, 563, 845
BfoI RGCGCY 1 cut(s) 659
BfuAI ACCTGC 3 cut(s) 101, 179, 293
BfuI GTATCC 1 cut(s) 584
BisI GCNGC 6 cut(s) 18, 97, 142, 191, 456, 948
BlsI GCNGC 6 cut(s) 19, 98, 143, 192, 457, 949
Bme1390I CCNGG 5 cut(s) 135, 317, 323, 537, 572
BmeT110I CYCGRG 1 cut(s) 611
BmgT120I GGNCC 4 cut(s) 131, 319, 330, 350
BmiI GGNNCC 4 cut(s) 6, 100, 127, 145
BmrFI CCNGG 5 cut(s) 135, 317, 323, 537, 572
BmrI ACTGGG 1 cut(s) 337
BmuI ACTGGG 1 cut(s) 337
Bpu10I CCTNAGC 1 cut(s) 110
BpuEI CTTGAG 1 cut(s) 563
BpuMI CCSGG 2 cut(s) 537, 572
BsaHI GRCGYC 1 cut(s) 549
BsaJI CCNNGG 5 cut(s) 133, 316, 552, 571, 670
BsaXI ACNNNNNCTCC 2 cut(s) 692, 722
Bsc4I CCNNNNNNNGG 4 cut(s) 140, 213, 322, 328
Bse118I RCCGGY 1 cut(s) 482
Bse1I ACTGG 1 cut(s) 332
BseBI CCWGG 3 cut(s) 135, 317, 323
BseDI CCNNGG 5 cut(s) 133, 316, 552, 571, 670
BseGI GGATG 4 cut(s) 269, 363, 417, 691
BseLI CCNNNNNNNGG 4 cut(s) 140, 213, 322, 328
BseMII CTCAG 1 cut(s) 124
BseNI ACTGG 1 cut(s) 332
BseRI GAGGAG 5 cut(s) 41, 44, 359, 382, 403
BseXI GCAGC 2 cut(s) 29, 202
BsgI GTGCAG 2 cut(s) 157, 271
Bsh1285I CGRYCG 1 cut(s) 627
BshFI GGCC 5 cut(s) 132, 321, 332, 351, 486
BsiEI CGRYCG 1 cut(s) 627
BsiHKCI CYCGRG 1 cut(s) 611
BsiSI CCGG 4 cut(s) 129, 483, 537, 571
BslFI GGGAC 2 cut(s) 456, 629
BslI CCNNNNNNNGG 4 cut(s) 140, 213, 322, 328
BsmAI GTCTC 1 cut(s) 695
BsmBI CGTCTC 1 cut(s) 695
BsmFI GGGAC 2 cut(s) 456, 629
BsnI GGCC 5 cut(s) 132, 321, 332, 351, 486
BsoBI CYCGRG 1 cut(s) 611
Bsp1286I GDGCHC 2 cut(s) 579, 918
Bsp143I GATC 1 cut(s) 936
BspACI CCGC 4 cut(s) 96, 141, 456, 678
BspANI GGCC 5 cut(s) 132, 321, 332, 351, 486
BspCNI CTCAG 1 cut(s) 123
BspLI GGNNCC 4 cut(s) 6, 100, 127, 145
BspMI ACCTGC 3 cut(s) 101, 179, 293
BsrFI RCCGGY 1 cut(s) 482
BsrI ACTGG 1 cut(s) 332
BssAI RCCGGY 1 cut(s) 482
BssECI CCNNGG 5 cut(s) 133, 316, 552, 571, 670
BssMI GATC 1 cut(s) 936
BssNI GRCGYC 1 cut(s) 549
BssT1I CCWWGG 1 cut(s) 552
Bst2UI CCWGG 3 cut(s) 135, 317, 323
Bst4CI ACNGT 1 cut(s) 830
Bst6I CTCTTC 1 cut(s) 506
BstACI GRCGYC 1 cut(s) 549
BstC8I GCNNGC 2 cut(s) 334, 560
BstDEI CTNAG 1 cut(s) 110
BstDSI CCRYGG 1 cut(s) 670
BstF5I GGATG 4 cut(s) 269, 363, 417, 691
BstH2I RGCGCY 1 cut(s) 659
BstHHI GCGC 2 cut(s) 455, 658
BstKTI GATC 1 cut(s) 939
BstMAI GTCTC 1 cut(s) 695
BstMBI GATC 1 cut(s) 936
BstMCI CGRYCG 1 cut(s) 627
BstMWI GCNNNNNNNGC 3 cut(s) 23, 138, 893
BstNI CCWGG 3 cut(s) 135, 317, 323
BstNSI RCATGY 1 cut(s) 471
BstSCI CCNGG 5 cut(s) 133, 315, 321, 535, 570
BstV1I GCAGC 2 cut(s) 29, 202
BsuI GTATCC 1 cut(s) 584
BsuRI GGCC 5 cut(s) 132, 321, 332, 351, 486
BtgI CCRYGG 1 cut(s) 670
BtsCI GGATG 4 cut(s) 269, 363, 417, 691
BtsIMutI CAGTG 1 cut(s) 835
BveI ACCTGC 3 cut(s) 101, 179, 293
Cac8I GCNNGC 2 cut(s) 334, 560
CfoI GCGC 2 cut(s) 455, 658
Cfr10I RCCGGY 1 cut(s) 482
Cfr13I GGNCC 4 cut(s) 131, 319, 330, 350
CseI GACGC 1 cut(s) 557
Csp6I GTAC 2 cut(s) 220, 646
CviAII CATG 3 cut(s) 277, 295, 468
CviQI GTAC 2 cut(s) 220, 646
DdeI CTNAG 1 cut(s) 110
DpnI GATC 1 cut(s) 938
DpnII GATC 1 cut(s) 936
Eam1104I CTCTTC 1 cut(s) 506
EarI CTCTTC 1 cut(s) 506
Eco130I CCWWGG 1 cut(s) 552
Eco24I GRGCYC 2 cut(s) 579, 918
Eco57I CTGAAG 2 cut(s) 680, 876
Eco88I CYCGRG 1 cut(s) 611
EcoRI GAATTC 1 cut(s) 859
EcoRII CCWGG 3 cut(s) 133, 315, 321
EcoT14I CCWWGG 1 cut(s) 552
EcoT38I GRGCYC 2 cut(s) 579, 918
ErhI CCWWGG 1 cut(s) 552
Esp3I CGTCTC 1 cut(s) 695
FaeI CATG 3 cut(s) 280, 298, 471
FaiI YATR 6 cut(s) 163, 278, 296, 469, 666, 747
FaqI GGGAC 2 cut(s) 456, 629
FatI CATG 3 cut(s) 276, 294, 467
FbaI TGATCA 1 cut(s) 936
FblI GTMKAC 1 cut(s) 528
Fnu4HI GCNGC 6 cut(s) 18, 97, 142, 191, 456, 948
FokI GGATG 4 cut(s) 256, 350, 404, 698
FriOI GRGCYC 2 cut(s) 579, 918
Fsp4HI GCNGC 6 cut(s) 18, 97, 142, 191, 456, 948
FspBI CTAG 4 cut(s) 14, 545, 563, 845
GlaI GCGC 2 cut(s) 454, 657
GluI GCNGC 6 cut(s) 18, 97, 142, 191, 456, 948
HaeII RGCGCY 1 cut(s) 659
HaeIII GGCC 5 cut(s) 132, 321, 332, 351, 486
HapII CCGG 4 cut(s) 129, 483, 537, 571
HgaI GACGC 1 cut(s) 557
HhaI GCGC 2 cut(s) 455, 658
Hin1I GRCGYC 1 cut(s) 549
Hin1II CATG 3 cut(s) 280, 298, 471
Hin6I GCGC 2 cut(s) 453, 656
HinP1I GCGC 2 cut(s) 453, 656
HinfI GANTC 2 cut(s) 410, 866
HpaII CCGG 4 cut(s) 129, 483, 537, 571
HphI GGTGA 4 cut(s) 220, 391, 472, 860
Hpy166II GTNNAC 3 cut(s) 282, 529, 881
Hpy188I TCNGA 4 cut(s) 201, 757, 808, 865
Hpy188III TCNNGA 2 cut(s) 839, 856
Hpy8I GTNNAC 3 cut(s) 282, 529, 881
Hpy99I CGWCG 1 cut(s) 605
HpyAV CCTTC 3 cut(s) 87, 163, 206
HpyCH4III ACNGT 1 cut(s) 830
HpyCH4V TGCA 4 cut(s) 174, 288, 298, 711
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 138, 893
HpyF3I CTNAG 1 cut(s) 110
Hsp92I GRCGYC 1 cut(s) 549
Hsp92II CATG 3 cut(s) 280, 298, 471
HspAI GCGC 2 cut(s) 453, 656
Ksp22I TGATCA 1 cut(s) 936
Kzo9I GATC 1 cut(s) 936
LmnI GCTCC 4 cut(s) 104, 131, 149, 198
Lsp1109I GCAGC 2 cut(s) 29, 202
MaeI CTAG 4 cut(s) 14, 545, 563, 845
MaeIII GTNAC 2 cut(s) 478, 848
MalI GATC 1 cut(s) 938
MboI GATC 1 cut(s) 936
MboII GAAGA 4 cut(s) 34, 493, 807, 882
MhlI GDGCHC 2 cut(s) 579, 918
MluCI AATT 2 cut(s) 823, 859
MlyI GAGTC 1 cut(s) 404
MmeI TCCRAC 1 cut(s) 181
MslI CAYNNNNRTG 1 cut(s) 293
MspI CCGG 4 cut(s) 129, 483, 537, 571
MspR9I CCNGG 5 cut(s) 135, 317, 323, 537, 572
MvaI CCWGG 3 cut(s) 135, 317, 323
MwoI GCNNNNNNNGC 3 cut(s) 23, 138, 893
NciI CCSGG 2 cut(s) 537, 572
NdeII GATC 1 cut(s) 936
NlaIII CATG 3 cut(s) 280, 298, 471
NlaIV GGNNCC 4 cut(s) 6, 100, 127, 145
NmeAIII GCCGAG 1 cut(s) 327
NmuCI GTSAC 2 cut(s) 478, 848
NspI RCATGY 1 cut(s) 471
PaqCI CACCTGC 2 cut(s) 101, 293
PciI ACATGT 1 cut(s) 467
PcsI WCGNNNNNNNCGW 1 cut(s) 555
PfeI GAWTC 1 cut(s) 866
PfoI TCCNGGA 1 cut(s) 535
PkrI GCNGC 6 cut(s) 19, 98, 143, 192, 457, 949
PleI GAGTC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 404
PscI ACATGT 1 cut(s) 467
Psp6I CCWGG 3 cut(s) 133, 315, 321
PspGI CCWGG 3 cut(s) 133, 315, 321
PspN4I GGNNCC 4 cut(s) 6, 100, 127, 145
PspPI GGNCC 4 cut(s) 131, 319, 330, 350
RsaI GTAC 2 cut(s) 221, 647
RsaNI GTAC 2 cut(s) 220, 646
RseI CAYNNNNRTG 1 cut(s) 293
SatI GCNGC 6 cut(s) 18, 97, 142, 191, 456, 948
Sau3AI GATC 1 cut(s) 936
Sau96I GGNCC 4 cut(s) 131, 319, 330, 350
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 5 cut(s) 135, 317, 323, 537, 572
SduI GDGCHC 2 cut(s) 579, 918
SmiMI CAYNNNNRTG 1 cut(s) 293
SmlI CTYRAG 1 cut(s) 578
SmoI CTYRAG 1 cut(s) 578
Sse9I AATT 2 cut(s) 823, 859
SsiI CCGC 4 cut(s) 96, 141, 456, 678
SspMI CTAG 4 cut(s) 14, 545, 563, 845
StyD4I CCNGG 5 cut(s) 133, 315, 321, 535, 570
StyI CCWWGG 1 cut(s) 552
TaaI ACNGT 1 cut(s) 830
TaqII GACCGA 1 cut(s) 641
TasI AATT 2 cut(s) 823, 859
TauI GCSGC 3 cut(s) 99, 144, 458
TfiI GAWTC 1 cut(s) 866
TscAI CASTG 1 cut(s) 835
TseFI GTSAC 2 cut(s) 478, 848
TseI GCWGC 3 cut(s) 17, 190, 947
Tsp45I GTSAC 2 cut(s) 478, 848
TspDTI ATGAA 1 cut(s) 352
TspGWI ACGGA 3 cut(s) 466, 589, 687
TspRI CASTG 1 cut(s) 835
XapI RAATTY 1 cut(s) 859
XceI RCATGY 1 cut(s) 471
XmiI GTMKAC 1 cut(s) 528
XspI CTAG 4 cut(s) 14, 545, 563, 845
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.