Rroxscaffold_2G00130930

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0014642
Physical Location & Seq
Reverse (-)
67430229 .. 67431649
1421 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00130930.1

Sequence Viewer

Length: 786 bp
ATGGAACCACAACGAGCATCATTAGCTCTTTCACTCATCATCTTCAACCTCCTCCTCACCACTACAACCGAAAACCCTTCATGCCAATCCACCTGCGGCTCCCCTCCACCTCAGCTACCCCTTCGGCTCAGGCCCCGGCTGCGGCTCCCCAACCTTCCAACCCTACATAACTTGCACAACAACCACCAAGCAGCTCCTTCTGACCACTCACACCGGATCATACCCCATCACCTCCATCTCCTACTCCACCAATACCCTAACCCTAACACCACCCTCCATCCTGCCCCTCCCAACCTGGGCCTAGACTGGGCCAGCACGTTCCAGCTCGGCCCATCAACTTTCATCCTCCTCTCCTGCCAACCCCCGACCTCCTCTCTCACCCTCAAAGACTCCTCCTCCCCAATCTGCGACCCCTCCTACTCCCACCTCTGCGCCGCCATCTACACGTGTCCCTCCGTCACCGCCCTCAACCTCCCTCTCTTCCCTCCCACCAACACTTGCTGTGTCTACTCCCCTGGAAACCTAGACGCCAAGGGAGAGCTAGACCTCCGCGGGCTCAAGTGTAAGGGATACACCTCCGTCGTGTCACTCGGGGATTACCCGACCGACCCGAACAAGTGGGAGTATGGGGTGGCGCTGAAGTATAACCACGGAGGCGGGTTGGATGGTGGCATTGTGGAGACGAAGTGCAAGAGTTGTGAGATGAGTGGCGGCTTGCCTACCACATACCCTATATGGAGTTGGTCTACCATTACACCAAACTATCGTCTTACTCTTCTTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

261

Amino Acids

28.5

Weight (kDa)

7.6

Isoelectric Point (pI)

45.8

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014642)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20600
malus_domestica MD01G1032500.v1.1 MD15G1322500.v1.1
prunus_persica Prupe.6G189700_v2.0.a1
pyrus_communis pycom01g05980 pycom15g28310
rosa_chinensis RchiOBHm_Chr2g0113031
rosa_laevigata RLG00000018031
rosa_multiflora Rmu_sc0002351.1_g000105 Rmu_sc0024545.1_g000002
rosa_roxburghii Rroxscaffold_2G00130930
rosa_rugosa Rorug02G0183300
rosa_samantha Rh2AG238500 Rh2BG252300 Rh2CG242500 Rh2DG246500
rosa_wichuraiana Rw2G018700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 101
Acc36I ACCTGC 1 cut(s) 101
AccI GTMKAC 2 cut(s) 507, 746
AccII CGCG 1 cut(s) 552
AciI CCGC 8 cut(s) 96, 142, 435, 462, 550, 552, 657, 711
AclWI GGATC 1 cut(s) 224
AcuI CTGAAG 1 cut(s) 659
AcvI CACGTG 1 cut(s) 447
AcyI GRCGYC 1 cut(s) 528
AfiI CCNNNNNNNGG 3 cut(s) 141, 296, 307
AflIII ACRYGT 2 cut(s) 444, 446
AgsI TTSAA 1 cut(s) 46
AjnI CCWGG 2 cut(s) 294, 514
AluBI AGCT 5 cut(s) 26, 115, 194, 325, 541
AluI AGCT 5 cut(s) 26, 115, 194, 325, 541
Alw26I GTCTC 1 cut(s) 674
AlwI GGATC 1 cut(s) 224
Ama87I CYCGRG 1 cut(s) 590
AoxI GGCC 4 cut(s) 131, 298, 309, 328
ApeKI GCWGC 2 cut(s) 139, 191
AspLEI GCGC 2 cut(s) 434, 637
AspS9I GGNCC 4 cut(s) 132, 298, 309, 329
AsuC2I CCSGG 1 cut(s) 136
AsuHPI GGTGA 4 cut(s) 49, 221, 370, 451
AvaI CYCGRG 1 cut(s) 590
BanII GRGCYC 1 cut(s) 558
BbrPI CACGTG 1 cut(s) 447
BbvCI CCTCAGC 1 cut(s) 111
BbvI GCAGC 2 cut(s) 126, 203
BccI CCATC 6 cut(s) 234, 243, 285, 340, 446, 659
BciT130I CCWGG 2 cut(s) 296, 516
BciVI GTATCC 1 cut(s) 563
BcnI CCSGG 1 cut(s) 136
BcoDI GTCTC 1 cut(s) 674
BfaI CTAG 3 cut(s) 302, 524, 542
BfoI RGCGCY 1 cut(s) 638
BfuAI ACCTGC 1 cut(s) 101
BfuI GTATCC 1 cut(s) 563
BisI GCNGC 6 cut(s) 97, 140, 143, 192, 435, 712
BlsI GCNGC 6 cut(s) 98, 141, 144, 193, 436, 713
Bme1390I CCNGG 3 cut(s) 136, 296, 516
BmeT110I CYCGRG 1 cut(s) 590
BmgT120I GGNCC 4 cut(s) 132, 298, 309, 329
BmiI GGNNCC 4 cut(s) 6, 100, 134, 146
BmrFI CCNGG 3 cut(s) 136, 296, 516
BmrI ACTGGG 1 cut(s) 316
BmsI GCATC 1 cut(s) 26
BmuI ACTGGG 1 cut(s) 316
Bpu10I CCTNAGC 2 cut(s) 111, 128
BpuEI CTTGAG 1 cut(s) 542
BpuMI CCSGG 1 cut(s) 136
BsaAI YACGTR 1 cut(s) 447
BsaHI GRCGYC 1 cut(s) 528
BsaJI CCNNGG 6 cut(s) 134, 295, 514, 531, 550, 649
BsaWI WCCGGW 1 cut(s) 213
BsaXI ACNNNNNCTCC 4 cut(s) 380, 410, 671, 701
Bsc4I CCNNNNNNNGG 3 cut(s) 141, 296, 307
Bse1I ACTGG 1 cut(s) 311
BseBI CCWGG 2 cut(s) 296, 516
BseDI CCNNGG 6 cut(s) 134, 295, 514, 531, 550, 649
BseGI GGATG 3 cut(s) 277, 342, 670
BseLI CCNNNNNNNGG 3 cut(s) 141, 296, 307
BseMII CTCAG 2 cut(s) 125, 142
BseNI ACTGG 1 cut(s) 311
BseRI GAGGAG 6 cut(s) 41, 44, 338, 361, 382, 385
BseXI GCAGC 2 cut(s) 126, 203
Bsh1236I CGCG 1 cut(s) 552
Bsh1285I CGRYCG 1 cut(s) 606
BshFI GGCC 4 cut(s) 133, 300, 311, 330
BsiEI CGRYCG 1 cut(s) 606
BsiHKCI CYCGRG 1 cut(s) 590
BsiSI CCGG 2 cut(s) 136, 214
BslFI GGGAC 1 cut(s) 435
BslI CCNNNNNNNGG 3 cut(s) 141, 296, 307
BsmAI GTCTC 1 cut(s) 674
BsmBI CGTCTC 1 cut(s) 674
BsmFI GGGAC 1 cut(s) 435
BsnI GGCC 4 cut(s) 133, 300, 311, 330
BsoBI CYCGRG 1 cut(s) 590
Bsp1286I GDGCHC 1 cut(s) 558
Bsp143I GATC 1 cut(s) 216
BspACI CCGC 8 cut(s) 96, 142, 435, 462, 550, 552, 657, 711
BspANI GGCC 4 cut(s) 133, 300, 311, 330
BspCNI CTCAG 2 cut(s) 124, 141
BspFNI CGCG 1 cut(s) 552
BspLI GGNNCC 4 cut(s) 6, 100, 134, 146
BspMI ACCTGC 1 cut(s) 101
BspPI GGATC 1 cut(s) 224
BsrI ACTGG 1 cut(s) 311
BssECI CCNNGG 6 cut(s) 134, 295, 514, 531, 550, 649
BssMI GATC 1 cut(s) 216
BssNI GRCGYC 1 cut(s) 528
BssT1I CCWWGG 1 cut(s) 531
Bst2UI CCWGG 2 cut(s) 296, 516
Bst6I CTCTTC 2 cut(s) 485, 780
BstACI GRCGYC 1 cut(s) 528
BstBAI YACGTR 1 cut(s) 447
BstC8I GCNNGC 3 cut(s) 313, 554, 716
BstDEI CTNAG 2 cut(s) 111, 128
BstDSI CCRYGG 2 cut(s) 550, 649
BstF5I GGATG 3 cut(s) 277, 342, 670
BstFNI CGCG 1 cut(s) 552
BstH2I RGCGCY 1 cut(s) 638
BstHHI GCGC 2 cut(s) 434, 637
BstKTI GATC 1 cut(s) 219
BstMAI GTCTC 1 cut(s) 674
BstMBI GATC 1 cut(s) 216
BstMCI CGRYCG 1 cut(s) 606
BstMWI GCNNNNNNNGC 2 cut(s) 23, 139
BstNI CCWGG 2 cut(s) 296, 516
BstSCI CCNGG 3 cut(s) 134, 294, 514
BstUI CGCG 1 cut(s) 552
BstV1I GCAGC 2 cut(s) 126, 203
BsuI GTATCC 1 cut(s) 563
BsuRI GGCC 4 cut(s) 133, 300, 311, 330
BtgI CCRYGG 2 cut(s) 550, 649
BtsCI GGATG 3 cut(s) 277, 342, 670
BveI ACCTGC 1 cut(s) 101
Cac8I GCNNGC 3 cut(s) 313, 554, 716
CfoI GCGC 2 cut(s) 434, 637
Cfr13I GGNCC 4 cut(s) 132, 298, 309, 329
Cfr42I CCGCGG 1 cut(s) 553
CseI GACGC 1 cut(s) 536
CviAII CATG 1 cut(s) 81
DdeI CTNAG 2 cut(s) 111, 128
DpnI GATC 1 cut(s) 218
DpnII GATC 1 cut(s) 216
Eam1104I CTCTTC 2 cut(s) 485, 780
EarI CTCTTC 2 cut(s) 485, 780
Eco130I CCWWGG 1 cut(s) 531
Eco24I GRGCYC 1 cut(s) 558
Eco57I CTGAAG 1 cut(s) 659
Eco72I CACGTG 1 cut(s) 447
Eco88I CYCGRG 1 cut(s) 590
EcoO109I RGGNCCY 1 cut(s) 132
EcoRII CCWGG 2 cut(s) 294, 514
EcoT14I CCWWGG 1 cut(s) 531
EcoT38I GRGCYC 1 cut(s) 558
ErhI CCWWGG 1 cut(s) 531
Esp3I CGTCTC 1 cut(s) 674
FaeI CATG 1 cut(s) 84
FaiI YATR 9 cut(s) 82, 168, 221, 627, 645, 727, 734, 736, 784
FaqI GGGAC 1 cut(s) 435
FatI CATG 1 cut(s) 80
FauI CCCGC 2 cut(s) 545, 650
FblI GTMKAC 2 cut(s) 507, 746
Fnu4HI GCNGC 6 cut(s) 97, 140, 143, 192, 435, 712
FokI GGATG 3 cut(s) 264, 329, 677
FriOI GRGCYC 1 cut(s) 558
Fsp4HI GCNGC 6 cut(s) 97, 140, 143, 192, 435, 712
FspBI CTAG 3 cut(s) 302, 524, 542
GlaI GCGC 2 cut(s) 433, 636
GluI GCNGC 6 cut(s) 97, 140, 143, 192, 435, 712
HaeII RGCGCY 1 cut(s) 638
HaeIII GGCC 4 cut(s) 133, 300, 311, 330
HapII CCGG 2 cut(s) 136, 214
HgaI GACGC 1 cut(s) 536
HhaI GCGC 2 cut(s) 434, 637
Hin1I GRCGYC 1 cut(s) 528
Hin1II CATG 1 cut(s) 84
Hin6I GCGC 2 cut(s) 432, 635
HinP1I GCGC 2 cut(s) 432, 635
HinfI GANTC 1 cut(s) 389
HpaII CCGG 2 cut(s) 136, 214
HphI GGTGA 4 cut(s) 49, 221, 370, 451
Hpy166II GTNNAC 2 cut(s) 508, 747
Hpy188I TCNGA 1 cut(s) 202
Hpy8I GTNNAC 2 cut(s) 508, 747
Hpy99I CGWCG 1 cut(s) 584
HpyAV CCTTC 4 cut(s) 87, 131, 164, 207
HpyCH4IV ACGT 2 cut(s) 317, 446
HpyCH4V TGCA 2 cut(s) 175, 690
HpyF10VI GCNNNNNNNGC 2 cut(s) 23, 139
HpyF3I CTNAG 2 cut(s) 111, 128
HpySE526I ACGT 2 cut(s) 317, 446
Hsp92I GRCGYC 1 cut(s) 528
Hsp92II CATG 1 cut(s) 84
HspAI GCGC 2 cut(s) 432, 635
KspI CCGCGG 1 cut(s) 553
Kzo9I GATC 1 cut(s) 216
LmnI GCTCC 3 cut(s) 104, 150, 199
Lsp1109I GCAGC 2 cut(s) 126, 203
LweI GCATC 1 cut(s) 26
MaeI CTAG 3 cut(s) 302, 524, 542
MaeII ACGT 2 cut(s) 317, 446
MaeIII GTNAC 2 cut(s) 457, 585
MalI GATC 1 cut(s) 218
MboI GATC 1 cut(s) 216
MboII GAAGA 3 cut(s) 34, 472, 767
MhlI GDGCHC 1 cut(s) 558
MlyI GAGTC 1 cut(s) 383
MmeI TCCRAC 2 cut(s) 182, 642
MspA1I CMGCKG 1 cut(s) 552
MspI CCGG 2 cut(s) 136, 214
MspR9I CCNGG 3 cut(s) 136, 296, 516
MvaI CCWGG 2 cut(s) 296, 516
MvnI CGCG 1 cut(s) 552
MwoI GCNNNNNNNGC 2 cut(s) 23, 139
NciI CCSGG 1 cut(s) 136
NdeII GATC 1 cut(s) 216
NlaIII CATG 1 cut(s) 84
NlaIV GGNNCC 4 cut(s) 6, 100, 134, 146
NmeAIII GCCGAG 1 cut(s) 306
NmuCI GTSAC 2 cut(s) 457, 585
PaqCI CACCTGC 1 cut(s) 101
PkrI GCNGC 6 cut(s) 98, 141, 144, 193, 436, 713
PleI GAGTC 1 cut(s) 383
PmaCI CACGTG 1 cut(s) 447
PmlI CACGTG 1 cut(s) 447
PpsI GAGTC 1 cut(s) 383
Ppu21I YACGTR 1 cut(s) 447
Psp6I CCWGG 2 cut(s) 294, 514
PspCI CACGTG 1 cut(s) 447
PspGI CCWGG 2 cut(s) 294, 514
PspN4I GGNNCC 4 cut(s) 6, 100, 134, 146
PspPI GGNCC 4 cut(s) 132, 298, 309, 329
SacII CCGCGG 1 cut(s) 553
SatI GCNGC 6 cut(s) 97, 140, 143, 192, 435, 712
Sau3AI GATC 1 cut(s) 216
Sau96I GGNCC 4 cut(s) 132, 298, 309, 329
SchI GAGTC 1 cut(s) 383
ScrFI CCNGG 3 cut(s) 136, 296, 516
SduI GDGCHC 1 cut(s) 558
SfaNI GCATC 1 cut(s) 26
Sfr303I CCGCGG 1 cut(s) 553
SgrBI CCGCGG 1 cut(s) 553
SmlI CTYRAG 1 cut(s) 557
SmoI CTYRAG 1 cut(s) 557
SsiI CCGC 8 cut(s) 96, 142, 435, 462, 550, 552, 657, 711
SspMI CTAG 3 cut(s) 302, 524, 542
StyD4I CCNGG 3 cut(s) 134, 294, 514
StyI CCWWGG 1 cut(s) 531
TaiI ACGT 2 cut(s) 320, 449
TaqII GACCGA 1 cut(s) 620
TauI GCSGC 4 cut(s) 99, 145, 437, 714
TseFI GTSAC 2 cut(s) 457, 585
TseI GCWGC 2 cut(s) 139, 191
Tsp45I GTSAC 2 cut(s) 457, 585
TspDTI ATGAA 2 cut(s) 69, 331
TspGWI ACGGA 3 cut(s) 445, 568, 666
XmiI GTMKAC 2 cut(s) 507, 746
XspI CTAG 3 cut(s) 302, 524, 542
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.