MD15G1322500.v1.1

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0014642
Physical Location & Seq
Forward (+)
33949177 .. 33950913
1737 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1322500.v1.1.491

Sequence Viewer

Length: 996 bp
ATGGTTGTTGTACCAGCTCTTTCAATATTCATCCTCCTCCTCCTCCTCTTCAACCACGGCTACATCACCACAGCCTTCGCCGCAGACTCGCCTGCTTGCAAGCCCACATGTGGATCCCTGCAACTGAGATACCCATTTGGCACAGGCCCTGGCTGTGGCTCACCAATTTTCCAACCCTACATAACCTGCGCATTTATCAATAATCAGCAGCAGCTCCTCCTCCTCACCACCCACACAGGCTCATACCCTATCACTTCCATTTCCTATGCCTCCCAAACCCTAATCCTAACCCCACCCTCCATGTCCAACTGCACCTCCATGCAACCCTCTTCCTCCAACTTCGGCCTTGACTGGGCCTCCCCATTTCAGCTCGGCCCATCAACTTTCATCCTCTTGTCCTGCCAGCCCCCCACCTCTTCCCTCACCCTCAAACCCTCCGGCATCCCCGTCTGTGATCCCTCCTACTCTTACCTTTGTGCTTCTATCTATACGTGTCCATCCGTCGTAGGTCTTGGCCTCCCCTTGTTCCCCCCAACAAACACATGCTGTGTGTATTCACCAGGGAATCTTGATGCTAAGGGTGAGTTGGACCTCAACGGGTTAAAGTGTGCCTCATTTACATCCGTGGTGTCTCTCGGAGACTACCCGACGGACCCTGTGCGGTGGGAGTATGGGGTGGCGTTGAAGTACAGTCACGGTGGTTTGGACAGTGGCATTGTGGATACAAAGTGCAAGAGCTGTGAGATGAGTGATGGGGTTTGTGGGTATAGGGTTGATGATCATGATCAGTTTCTTTGTGTGTGTAAGAATGGTTACAACACCTCATCAGATTGCCATAATAATTACACTCCGGATTCGGAACTGCTTTGGGGCTCCGGCGCCTGTGATAATCATTTACCAGTTAATATTTGGAAAATGTGGTCTGCGCTTGTGGCTGGCTTGATGATCATAATGCTTGAGAGAGAGATGGCTTTTACTTGGAAATGCTTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

332

Amino Acids

35.81

Weight (kDa)

5.06

Isoelectric Point (pI)

43.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 33 - 96 1e-10 Wall-associated receptor kinase galacturonan-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014642)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20600
malus_domestica MD01G1032500.v1.1 MD15G1322500.v1.1
prunus_persica Prupe.6G189700_v2.0.a1
pyrus_communis pycom01g05980 pycom15g28310
rosa_chinensis RchiOBHm_Chr2g0113031
rosa_laevigata RLG00000018031
rosa_multiflora Rmu_sc0002351.1_g000105 Rmu_sc0024545.1_g000002
rosa_roxburghii Rroxscaffold_2G00130930
rosa_rugosa Rorug02G0183300
rosa_samantha Rh2AG238500 Rh2BG252300 Rh2CG242500 Rh2DG246500
rosa_wichuraiana Rw2G018700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 190
Acc36I ACCTGC 1 cut(s) 194
AccB1I GGYRCC 1 cut(s) 878
AccIII TCCGGA 1 cut(s) 850
AciI CCGC 2 cut(s) 81, 661
AclWI GGATC 3 cut(s) 108, 121, 449
AcyI GRCGYC 1 cut(s) 879
AfaI GTAC 2 cut(s) 12, 689
AfiI CCNNNNNNNGG 3 cut(s) 110, 155, 352
AflIII ACRYGT 2 cut(s) 107, 491
AgsI TTSAA 3 cut(s) 24, 52, 685
AjnI CCWGG 2 cut(s) 148, 559
AluBI AGCT 4 cut(s) 17, 214, 370, 738
AluI AGCT 4 cut(s) 17, 214, 370, 738
Alw26I GTCTC 2 cut(s) 633, 636
AlwI GGATC 3 cut(s) 108, 121, 449
AlwNI CAGNNNCTG 1 cut(s) 149
Aor13HI TCCGGA 1 cut(s) 850
AoxI GGCC 5 cut(s) 145, 343, 354, 373, 514
ApeKI GCWGC 2 cut(s) 208, 211
Asp700I GAANNNNTTC 1 cut(s) 986
AspLEI GCGC 3 cut(s) 191, 881, 928
AspS9I GGNCC 5 cut(s) 146, 354, 374, 589, 652
AsuHPI GGTGA 6 cut(s) 58, 153, 217, 415, 549, 593
AvaII GGWCC 2 cut(s) 589, 652
BamHI GGATCC 1 cut(s) 113
BanI GGYRCC 1 cut(s) 878
BanII GRGCYC 1 cut(s) 875
BbvI GCAGC 2 cut(s) 220, 223
BccI CCATC 4 cut(s) 385, 505, 746, 961
BceAI ACGGC 1 cut(s) 73
BciT130I CCWGG 2 cut(s) 150, 561
BciVI GTATCC 1 cut(s) 715
BclI TGATCA 3 cut(s) 778, 784, 945
BcoDI GTCTC 2 cut(s) 633, 636
BfoI RGCGCY 1 cut(s) 882
BfuAI ACCTGC 1 cut(s) 194
BfuI GTATCC 1 cut(s) 715
BisI GCNGC 3 cut(s) 81, 209, 212
BlsI GCNGC 3 cut(s) 82, 210, 213
Bme1390I CCNGG 2 cut(s) 150, 561
Bme18I GGWCC 2 cut(s) 589, 652
BmgT120I GGNCC 5 cut(s) 146, 354, 374, 589, 652
BmiI GGNNCC 4 cut(s) 115, 654, 874, 880
BmrFI CCNGG 2 cut(s) 150, 561
BmrI ACTGGG 1 cut(s) 361
BmsI GCATC 2 cut(s) 450, 562
BmuI ACTGGG 1 cut(s) 361
Bpu10I CCTNAGC 1 cut(s) 576
BpuEI CTTGAG 1 cut(s) 977
BsaAI YACGTR 1 cut(s) 492
BsaBI GATNNNNATC 1 cut(s) 783
BsaHI GRCGYC 1 cut(s) 879
BsaJI CCNNGG 4 cut(s) 55, 148, 560, 624
BsaWI WCCGGW 1 cut(s) 850
BsaXI ACNNNNNCTCC 4 cut(s) 299, 329, 341, 371
Bsc4I CCNNNNNNNGG 3 cut(s) 110, 155, 352
Bse1I ACTGG 2 cut(s) 356, 899
Bse8I GATNNNNATC 1 cut(s) 783
BseAI TCCGGA 1 cut(s) 850
BseBI CCWGG 2 cut(s) 150, 561
BseDI CCNNGG 4 cut(s) 55, 148, 560, 624
BseGI GGATG 5 cut(s) 30, 387, 441, 497, 620
BseJI GATNNNNATC 1 cut(s) 783
BseLI CCNNNNNNNGG 3 cut(s) 110, 155, 352
BseMII CTCAG 1 cut(s) 116
BseNI ACTGG 2 cut(s) 356, 899
BseRI GAGGAG 7 cut(s) 26, 29, 32, 35, 206, 209, 212
BseXI GCAGC 2 cut(s) 220, 223
BsgI GTGCAG 1 cut(s) 295
BshFI GGCC 5 cut(s) 147, 345, 356, 375, 516
BshNI GGYRCC 1 cut(s) 878
BsiSI CCGG 3 cut(s) 438, 851, 876
BslI CCNNNNNNNGG 3 cut(s) 110, 155, 352
BsmAI GTCTC 2 cut(s) 633, 636
BsnI GGCC 5 cut(s) 147, 345, 356, 375, 516
Bsp1286I GDGCHC 1 cut(s) 875
Bsp13I TCCGGA 1 cut(s) 850
Bsp143I GATC 5 cut(s) 113, 454, 778, 784, 945
BspACI CCGC 2 cut(s) 81, 661
BspANI GGCC 5 cut(s) 147, 345, 356, 375, 516
BspCNI CTCAG 1 cut(s) 117
BspEI TCCGGA 1 cut(s) 850
BspHI TCATGA 1 cut(s) 781
BspLI GGNNCC 4 cut(s) 115, 654, 874, 880
BspMI ACCTGC 1 cut(s) 194
BspPI GGATC 3 cut(s) 108, 121, 449
BspT107I GGYRCC 1 cut(s) 878
BsrI ACTGG 2 cut(s) 356, 899
BssECI CCNNGG 4 cut(s) 55, 148, 560, 624
BssMI GATC 5 cut(s) 113, 454, 778, 784, 945
BssNI GRCGYC 1 cut(s) 879
Bst2UI CCWGG 2 cut(s) 150, 561
Bst4CI ACNGT 3 cut(s) 692, 698, 710
Bst6I CTCTTC 3 cut(s) 53, 334, 421
BstACI GRCGYC 1 cut(s) 879
BstBAI YACGTR 1 cut(s) 492
BstC8I GCNNGC 5 cut(s) 93, 97, 101, 404, 937
BstDEI CTNAG 2 cut(s) 125, 576
BstDSI CCRYGG 2 cut(s) 55, 624
BstF5I GGATG 5 cut(s) 30, 387, 441, 497, 620
BstH2I RGCGCY 1 cut(s) 882
BstHHI GCGC 3 cut(s) 191, 881, 928
BstKTI GATC 5 cut(s) 116, 457, 781, 787, 948
BstMAI GTCTC 2 cut(s) 633, 636
BstMBI GATC 5 cut(s) 113, 454, 778, 784, 945
BstMWI GCNNNNNNNGC 2 cut(s) 80, 932
BstNI CCWGG 2 cut(s) 150, 561
BstNSI RCATGY 2 cut(s) 111, 546
BstSCI CCNGG 2 cut(s) 148, 559
BstV1I GCAGC 2 cut(s) 220, 223
BstX2I RGATCY 1 cut(s) 113
BstYI RGATCY 1 cut(s) 113
BsuI GTATCC 1 cut(s) 715
BsuRI GGCC 5 cut(s) 147, 345, 356, 375, 516
BtgI CCRYGG 2 cut(s) 55, 624
BtsCI GGATG 5 cut(s) 30, 387, 441, 497, 620
BtsIMutI CAGTG 1 cut(s) 715
BveI ACCTGC 1 cut(s) 194
Cac8I GCNNGC 5 cut(s) 93, 97, 101, 404, 937
CaiI CAGNNNCTG 1 cut(s) 149
CciI TCATGA 1 cut(s) 781
CfoI GCGC 3 cut(s) 191, 881, 928
Cfr13I GGNCC 5 cut(s) 146, 354, 374, 589, 652
Csp6I GTAC 2 cut(s) 11, 688
CviAII CATG 5 cut(s) 108, 301, 319, 543, 782
CviQI GTAC 2 cut(s) 11, 688
DdeI CTNAG 2 cut(s) 125, 576
DinI GGCGCC 1 cut(s) 880
DpnI GATC 5 cut(s) 115, 456, 780, 786, 947
DpnII GATC 5 cut(s) 113, 454, 778, 784, 945
Eam1104I CTCTTC 3 cut(s) 53, 334, 421
EarI CTCTTC 3 cut(s) 53, 334, 421
Eco24I GRGCYC 1 cut(s) 875
Eco47I GGWCC 2 cut(s) 589, 652
EcoO109I RGGNCCY 1 cut(s) 146
EcoRII CCWGG 2 cut(s) 148, 559
EcoT38I GRGCYC 1 cut(s) 875
EgeI GGCGCC 1 cut(s) 880
EheI GGCGCC 1 cut(s) 880
FaeI CATG 5 cut(s) 111, 304, 322, 546, 785
FatI CATG 5 cut(s) 107, 300, 318, 542, 781
FbaI TGATCA 3 cut(s) 778, 784, 945
Fnu4HI GCNGC 3 cut(s) 81, 209, 212
FokI GGATG 5 cut(s) 17, 374, 428, 484, 607
FriOI GRGCYC 1 cut(s) 875
Fsp4HI GCNGC 3 cut(s) 81, 209, 212
FspI TGCGCA 1 cut(s) 190
GlaI GCGC 3 cut(s) 190, 880, 927
GluI GCNGC 3 cut(s) 81, 209, 212
HaeII RGCGCY 1 cut(s) 882
HaeIII GGCC 5 cut(s) 147, 345, 356, 375, 516
HapII CCGG 3 cut(s) 438, 851, 876
HhaI GCGC 3 cut(s) 191, 881, 928
Hin1I GRCGYC 1 cut(s) 879
Hin1II CATG 5 cut(s) 111, 304, 322, 546, 785
Hin6I GCGC 3 cut(s) 189, 879, 926
HinP1I GCGC 3 cut(s) 189, 879, 926
HinfI GANTC 3 cut(s) 86, 565, 854
HpaII CCGG 3 cut(s) 438, 851, 876
HphI GGTGA 6 cut(s) 58, 153, 217, 415, 549, 593
Hpy188I TCNGA 3 cut(s) 638, 829, 859
Hpy188III TCNNGA 3 cut(s) 569, 782, 851
Hpy99I CGWCG 2 cut(s) 506, 652
HpyAV CCTTC 1 cut(s) 85
HpyCH4III ACNGT 3 cut(s) 692, 698, 710
HpyCH4IV ACGT 1 cut(s) 491
HpyCH4V TGCA 5 cut(s) 99, 121, 312, 322, 732
HpyF10VI GCNNNNNNNGC 2 cut(s) 80, 932
HpyF3I CTNAG 2 cut(s) 125, 576
HpySE526I ACGT 1 cut(s) 491
Hsp92I GRCGYC 1 cut(s) 879
Hsp92II CATG 5 cut(s) 111, 304, 322, 546, 785
HspAI GCGC 3 cut(s) 189, 879, 926
KasI GGCGCC 1 cut(s) 878
Kpn2I TCCGGA 1 cut(s) 850
Ksp22I TGATCA 3 cut(s) 778, 784, 945
Kzo9I GATC 5 cut(s) 113, 454, 778, 784, 945
LmnI GCTCC 2 cut(s) 219, 878
Lsp1109I GCAGC 2 cut(s) 220, 223
LweI GCATC 2 cut(s) 450, 562
MaeII ACGT 1 cut(s) 491
MaeIII GTNAC 2 cut(s) 692, 812
MalI GATC 5 cut(s) 115, 456, 780, 786, 947
MboI GATC 5 cut(s) 113, 454, 778, 784, 945
MboII GAAGA 3 cut(s) 40, 321, 408
MflI RGATCY 1 cut(s) 113
MhlI GDGCHC 1 cut(s) 875
MluCI AATT 2 cut(s) 165, 841
Mly113I GGCGCC 1 cut(s) 879
MlyI GAGTC 1 cut(s) 80
MmeI TCCRAC 4 cut(s) 196, 330, 360, 567
MroI TCCGGA 1 cut(s) 850
MroXI GAANNNNTTC 1 cut(s) 986
MseI TTAA 2 cut(s) 602, 903
MslI CAYNNNNRTG 1 cut(s) 317
MspI CCGG 3 cut(s) 438, 851, 876
MspR9I CCNGG 2 cut(s) 150, 561
MvaI CCWGG 2 cut(s) 150, 561
MwoI GCNNNNNNNGC 2 cut(s) 80, 932
NarI GGCGCC 1 cut(s) 879
NdeII GATC 5 cut(s) 113, 454, 778, 784, 945
NlaIII CATG 5 cut(s) 111, 304, 322, 546, 785
NlaIV GGNNCC 4 cut(s) 115, 654, 874, 880
NmeAIII GCCGAG 1 cut(s) 351
NmuCI GTSAC 1 cut(s) 692
NsbI TGCGCA 1 cut(s) 190
NspI RCATGY 2 cut(s) 111, 546
PagI TCATGA 1 cut(s) 781
PciI ACATGT 1 cut(s) 107
PdmI GAANNNNTTC 1 cut(s) 986
PfeI GAWTC 2 cut(s) 565, 854
PkrI GCNGC 3 cut(s) 82, 210, 213
PleI GAGTC 1 cut(s) 80
PluTI GGCGCC 1 cut(s) 882
PpsI GAGTC 1 cut(s) 80
Ppu21I YACGTR 1 cut(s) 492
PscI ACATGT 1 cut(s) 107
Psp6I CCWGG 2 cut(s) 148, 559
PspGI CCWGG 2 cut(s) 148, 559
PspN4I GGNNCC 4 cut(s) 115, 654, 874, 880
PspPI GGNCC 5 cut(s) 146, 354, 374, 589, 652
PstNI CAGNNNCTG 1 cut(s) 149
PsuI RGATCY 1 cut(s) 113
RsaI GTAC 2 cut(s) 12, 689
RsaNI GTAC 2 cut(s) 11, 688
RseI CAYNNNNRTG 1 cut(s) 317
SaqAI TTAA 2 cut(s) 602, 903
SatI GCNGC 3 cut(s) 81, 209, 212
Sau3AI GATC 5 cut(s) 113, 454, 778, 784, 945
Sau96I GGNCC 5 cut(s) 146, 354, 374, 589, 652
SchI GAGTC 1 cut(s) 80
ScrFI CCNGG 2 cut(s) 150, 561
SduI GDGCHC 1 cut(s) 875
SfaNI GCATC 2 cut(s) 450, 562
SfoI GGCGCC 1 cut(s) 880
SinI GGWCC 2 cut(s) 589, 652
SmiMI CAYNNNNRTG 1 cut(s) 317
SmlI CTYRAG 1 cut(s) 956
SmoI CTYRAG 1 cut(s) 956
Sse9I AATT 2 cut(s) 165, 841
SsiI CCGC 2 cut(s) 81, 661
SspDI GGCGCC 1 cut(s) 878
SspI AATATT 2 cut(s) 27, 907
StyD4I CCNGG 2 cut(s) 148, 559
TaaI ACNGT 3 cut(s) 692, 698, 710
TaiI ACGT 1 cut(s) 494
TasI AATT 2 cut(s) 165, 841
TatI WGTACW 1 cut(s) 687
TauI GCSGC 1 cut(s) 83
TfiI GAWTC 2 cut(s) 565, 854
Tru1I TTAA 2 cut(s) 602, 903
Tru9I TTAA 2 cut(s) 602, 903
TscAI CASTG 1 cut(s) 715
TseFI GTSAC 1 cut(s) 692
TseI GCWGC 2 cut(s) 208, 211
Tsp45I GTSAC 1 cut(s) 692
TspDTI ATGAA 2 cut(s) 19, 376
TspGWI ACGGA 3 cut(s) 490, 613, 665
TspRI CASTG 1 cut(s) 715
VpaK11BI GGWCC 2 cut(s) 589, 652
XceI RCATGY 2 cut(s) 111, 546
XcmI CCANNNNNNNNNTGG 1 cut(s) 906
XmnI GAANNNNTTC 1 cut(s) 986
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.