Rh2AG238500

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0014642
Physical Location & Seq
Forward (+)
26037709 .. 26039253
1545 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG238500.1

Sequence Viewer

Length: 957 bp
ATGGAACCACAGAGAGCATCATTAGCTCTTTCACTCATCATCTTCAACCTCCTCCTCACCACCACCACCCAATACCCTTCATGTCAATCCACCTGCGGCTCCCTCCACCTTAGCTACCCCTTCGGCTCCGGCCCCGGCTGCGGCTCCCCAACCTTCCAACCCTACATAACTTGCACAACAACCACCAAGCAGCTCCTTATTCTGACCACTCACACCGGATCATACCCCATCACCTCCATCTCCTACTCCACCAACACCCTAACCCTAACACCACCCTCCATGTCCACCTGCACCTCCATGCAACCCTGCCCCTCCAACCTGGGCCTCGACTGGGCCAGCCCGTTCCAGCTCGGCCCATCAACTTTCATCCTCCTCTCCTGCCAAGCCCCTACCTCCTCTCTCACCCTCAAAGACTCCTCCTCCCCAATCTGCGACCCCTCCTACTCCCACCTCTGCGCGGCCATCTACACGTGTCCCTCCGTCACCGGCCTCAACCTCCCTCTCCTCCCTCCCACCAACACTTGCTGTGTCTACTCCCCTGGAAACCTAGACACCAAGGGCGAGCTAGACCTCCGGGGGCTCAAGTGTAAGGGATACACCTCCGTCGTGTCGCTCGGGGATTACCCAACCGACCCGAACAAGTGGGAGTATGGGGTGGCGCTGAAGTATAACCACGGAGGCGAGTTGGATGGTGGCATTGTGGAGACAAAGTGCAAGAGTTGTGAGATGAGTGGTGGCGTTTGTGGTTATGCTACTTCTGATAATTCGTTTCTTTGTGTTTGTAAAAATGGCGACTACAACACCACTTTGGATTGTTCTTCTGATAATTACAGTGGCGTTCAAGAACAAGGTGTCATATGGAGTCCGGAATCTGGCTCTGATTTACCAGCTTCTTCAGCTTGGAAAATATGGTTTGGGCTCTTGGCTCTGGCTGAGCTGATCATTTTAACAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

318

Amino Acids

33.78

Weight (kDa)

4.75

Isoelectric Point (pI)

42.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 28 - 89 4.9e-12 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 234 - 263 3.1e-07 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014642)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20600
malus_domestica MD01G1032500.v1.1 MD15G1322500.v1.1
prunus_persica Prupe.6G189700_v2.0.a1
pyrus_communis pycom01g05980 pycom15g28310
rosa_chinensis RchiOBHm_Chr2g0113031
rosa_laevigata RLG00000018031
rosa_multiflora Rmu_sc0002351.1_g000105 Rmu_sc0024545.1_g000002
rosa_roxburghii Rroxscaffold_2G00130930
rosa_rugosa Rorug02G0183300
rosa_samantha Rh2AG238500 Rh2BG252300 Rh2CG242500 Rh2DG246500
rosa_wichuraiana Rw2G018700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 101, 296
Acc36I ACCTGC 2 cut(s) 101, 296
AccI GTMKAC 1 cut(s) 531
AccII CGCG 1 cut(s) 458
AccIII TCCGGA 1 cut(s) 865
AciI CCGC 3 cut(s) 96, 141, 458
AclWI GGATC 1 cut(s) 226
AcoI YGGCCR 1 cut(s) 459
AcuI CTGAAG 2 cut(s) 683, 879
AcvI CACGTG 1 cut(s) 471
AfiI CCNNNNNNNGG 4 cut(s) 140, 331, 457, 872
AflIII ACRYGT 2 cut(s) 468, 470
AgsI TTSAA 2 cut(s) 46, 842
AjnI CCWGG 2 cut(s) 318, 538
AluBI AGCT 9 cut(s) 26, 114, 193, 349, 565, 890, 899, 937, 953
AluI AGCT 9 cut(s) 26, 114, 193, 349, 565, 890, 899, 937, 953
Alw26I GTCTC 1 cut(s) 698
AlwI GGATC 1 cut(s) 226
Ama87I CYCGRG 1 cut(s) 614
Aor13HI TCCGGA 1 cut(s) 865
AoxI GGCC 6 cut(s) 130, 322, 333, 352, 459, 487
ApeKI GCWGC 2 cut(s) 138, 190
AspLEI GCGC 2 cut(s) 458, 661
AspS9I GGNCC 4 cut(s) 131, 322, 333, 353
AsuC2I CCSGG 2 cut(s) 135, 575
AsuHPI GGTGA 4 cut(s) 49, 223, 394, 475
AvaI CYCGRG 1 cut(s) 614
BanII GRGCYC 2 cut(s) 582, 921
BbrPI CACGTG 1 cut(s) 471
BbvI GCAGC 2 cut(s) 125, 202
BccI CCATC 5 cut(s) 236, 245, 364, 470, 683
BciT130I CCWGG 2 cut(s) 320, 540
BciVI GTATCC 1 cut(s) 587
BclI TGATCA 1 cut(s) 939
BcnI CCSGG 2 cut(s) 135, 575
BcoDI GTCTC 1 cut(s) 698
BfaI CTAG 2 cut(s) 548, 566
BfoI RGCGCY 1 cut(s) 662
BfuAI ACCTGC 2 cut(s) 101, 296
BfuI GTATCC 1 cut(s) 587
BisI GCNGC 5 cut(s) 97, 139, 142, 191, 459
BlpI GCTNAGC 1 cut(s) 933
BlsI GCNGC 5 cut(s) 98, 140, 143, 192, 460
Bme1390I CCNGG 4 cut(s) 135, 320, 540, 575
BmeT110I CYCGRG 1 cut(s) 614
BmgT120I GGNCC 4 cut(s) 131, 322, 333, 353
BmiI GGNNCC 5 cut(s) 6, 100, 127, 133, 145
BmrFI CCNGG 4 cut(s) 135, 320, 540, 575
BmrI ACTGGG 1 cut(s) 340
BmsI GCATC 1 cut(s) 26
BmuI ACTGGG 1 cut(s) 340
Bpu10I CCTNAGC 1 cut(s) 110
Bpu1102I GCTNAGC 1 cut(s) 933
BpuEI CTTGAG 1 cut(s) 566
BpuMI CCSGG 2 cut(s) 135, 575
BsaAI YACGTR 1 cut(s) 471
BsaJI CCNNGG 6 cut(s) 133, 319, 538, 555, 574, 673
BsaWI WCCGGW 2 cut(s) 215, 865
BsaXI ACNNNNNCTCC 4 cut(s) 404, 434, 695, 725
Bsc4I CCNNNNNNNGG 4 cut(s) 140, 331, 457, 872
Bse118I RCCGGY 1 cut(s) 485
Bse1I ACTGG 1 cut(s) 335
BseAI TCCGGA 1 cut(s) 865
BseBI CCWGG 2 cut(s) 320, 540
BseDI CCNNGG 6 cut(s) 133, 319, 538, 555, 574, 673
BseGI GGATG 2 cut(s) 366, 694
BseLI CCNNNNNNNGG 4 cut(s) 140, 331, 457, 872
BseMII CTCAG 1 cut(s) 924
BseNI ACTGG 1 cut(s) 335
BseRI GAGGAG 7 cut(s) 41, 44, 362, 385, 406, 409, 494
BseXI GCAGC 2 cut(s) 125, 202
BsgI GTGCAG 1 cut(s) 274
Bsh1236I CGCG 1 cut(s) 458
BshFI GGCC 6 cut(s) 132, 324, 335, 354, 461, 489
BsiHKCI CYCGRG 1 cut(s) 614
BsiSI CCGG 6 cut(s) 129, 135, 216, 486, 574, 866
BslFI GGGAC 1 cut(s) 459
BslI CCNNNNNNNGG 4 cut(s) 140, 331, 457, 872
BsmAI GTCTC 1 cut(s) 698
BsmFI GGGAC 1 cut(s) 459
BsnI GGCC 6 cut(s) 132, 324, 335, 354, 461, 489
BsoBI CYCGRG 1 cut(s) 614
Bsp1286I GDGCHC 2 cut(s) 582, 921
Bsp13I TCCGGA 1 cut(s) 865
Bsp143I GATC 2 cut(s) 218, 939
Bsp1720I GCTNAGC 1 cut(s) 933
BspACI CCGC 3 cut(s) 96, 141, 458
BspANI GGCC 6 cut(s) 132, 324, 335, 354, 461, 489
BspCNI CTCAG 1 cut(s) 925
BspEI TCCGGA 1 cut(s) 865
BspFNI CGCG 1 cut(s) 458
BspLI GGNNCC 5 cut(s) 6, 100, 127, 133, 145
BspMI ACCTGC 2 cut(s) 101, 296
BspPI GGATC 1 cut(s) 226
BsrFI RCCGGY 1 cut(s) 485
BsrI ACTGG 1 cut(s) 335
BssAI RCCGGY 1 cut(s) 485
BssECI CCNNGG 6 cut(s) 133, 319, 538, 555, 574, 673
BssMI GATC 2 cut(s) 218, 939
BssT1I CCWWGG 1 cut(s) 555
Bst2UI CCWGG 2 cut(s) 320, 540
Bst4CI ACNGT 1 cut(s) 833
BstBAI YACGTR 1 cut(s) 471
BstC8I GCNNGC 2 cut(s) 337, 563
BstDEI CTNAG 2 cut(s) 110, 933
BstDSI CCRYGG 1 cut(s) 673
BstF5I GGATG 2 cut(s) 366, 694
BstFNI CGCG 1 cut(s) 458
BstH2I RGCGCY 1 cut(s) 662
BstHHI GCGC 2 cut(s) 458, 661
BstKTI GATC 2 cut(s) 221, 942
BstMAI GTCTC 1 cut(s) 698
BstMBI GATC 2 cut(s) 218, 939
BstMWI GCNNNNNNNGC 3 cut(s) 23, 138, 896
BstNI CCWGG 2 cut(s) 320, 540
BstSCI CCNGG 4 cut(s) 133, 318, 538, 573
BstUI CGCG 1 cut(s) 458
BstV1I GCAGC 2 cut(s) 125, 202
BsuI GTATCC 1 cut(s) 587
BsuRI GGCC 6 cut(s) 132, 324, 335, 354, 461, 489
BtgI CCRYGG 1 cut(s) 673
BtsCI GGATG 2 cut(s) 366, 694
BtsIMutI CAGTG 1 cut(s) 838
BveI ACCTGC 2 cut(s) 101, 296
Cac8I GCNNGC 2 cut(s) 337, 563
CfoI GCGC 2 cut(s) 458, 661
Cfr10I RCCGGY 1 cut(s) 485
Cfr13I GGNCC 4 cut(s) 131, 322, 333, 353
CviAII CATG 3 cut(s) 81, 280, 298
DdeI CTNAG 2 cut(s) 110, 933
DpnI GATC 2 cut(s) 220, 941
DpnII GATC 2 cut(s) 218, 939
EaeI YGGCCR 1 cut(s) 459
Eco130I CCWWGG 1 cut(s) 555
Eco24I GRGCYC 2 cut(s) 582, 921
Eco57I CTGAAG 2 cut(s) 683, 879
Eco72I CACGTG 1 cut(s) 471
Eco88I CYCGRG 1 cut(s) 614
EcoRII CCWGG 2 cut(s) 318, 538
EcoT14I CCWWGG 1 cut(s) 555
EcoT38I GRGCYC 2 cut(s) 582, 921
ErhI CCWWGG 1 cut(s) 555
FaeI CATG 3 cut(s) 84, 283, 301
FaqI GGGAC 1 cut(s) 459
FatI CATG 3 cut(s) 80, 279, 297
FauNDI CATATG 1 cut(s) 857
FbaI TGATCA 1 cut(s) 939
FblI GTMKAC 1 cut(s) 531
Fnu4HI GCNGC 5 cut(s) 97, 139, 142, 191, 459
FokI GGATG 2 cut(s) 353, 701
FriOI GRGCYC 2 cut(s) 582, 921
Fsp4HI GCNGC 5 cut(s) 97, 139, 142, 191, 459
FspBI CTAG 2 cut(s) 548, 566
GlaI GCGC 2 cut(s) 457, 660
GluI GCNGC 5 cut(s) 97, 139, 142, 191, 459
HaeII RGCGCY 1 cut(s) 662
HaeIII GGCC 6 cut(s) 132, 324, 335, 354, 461, 489
HapII CCGG 6 cut(s) 129, 135, 216, 486, 574, 866
HhaI GCGC 2 cut(s) 458, 661
Hin1II CATG 3 cut(s) 84, 283, 301
Hin6I GCGC 2 cut(s) 456, 659
HinP1I GCGC 2 cut(s) 456, 659
HinfI GANTC 3 cut(s) 413, 862, 869
HpaII CCGG 6 cut(s) 129, 135, 216, 486, 574, 866
HphI GGTGA 4 cut(s) 49, 223, 394, 475
Hpy166II GTNNAC 2 cut(s) 285, 532
Hpy188I TCNGA 4 cut(s) 204, 760, 823, 880
Hpy188III TCNNGA 2 cut(s) 842, 866
Hpy8I GTNNAC 2 cut(s) 285, 532
Hpy99I CGWCG 1 cut(s) 608
HpyAV CCTTC 3 cut(s) 87, 130, 163
HpyCH4III ACNGT 1 cut(s) 833
HpyCH4IV ACGT 1 cut(s) 470
HpyCH4V TGCA 4 cut(s) 174, 291, 301, 714
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 138, 896
HpyF3I CTNAG 2 cut(s) 110, 933
HpySE526I ACGT 1 cut(s) 470
Hsp92II CATG 3 cut(s) 84, 283, 301
HspAI GCGC 2 cut(s) 456, 659
Kpn2I TCCGGA 1 cut(s) 865
Ksp22I TGATCA 1 cut(s) 939
Kzo9I GATC 2 cut(s) 218, 939
LmnI GCTCC 4 cut(s) 104, 131, 149, 198
Lsp1109I GCAGC 2 cut(s) 125, 202
LweI GCATC 1 cut(s) 26
MaeI CTAG 2 cut(s) 548, 566
MaeII ACGT 1 cut(s) 470
MaeIII GTNAC 1 cut(s) 481
MalI GATC 2 cut(s) 220, 941
MboI GATC 2 cut(s) 218, 939
MboII GAAGA 3 cut(s) 34, 810, 885
MhlI GDGCHC 2 cut(s) 582, 921
MluCI AATT 2 cut(s) 763, 826
MlyI GAGTC 2 cut(s) 407, 871
MmeI TCCRAC 3 cut(s) 181, 339, 666
MroI TCCGGA 1 cut(s) 865
MseI TTAA 1 cut(s) 947
MslI CAYNNNNRTG 1 cut(s) 296
MspI CCGG 6 cut(s) 129, 135, 216, 486, 574, 866
MspR9I CCNGG 4 cut(s) 135, 320, 540, 575
MvaI CCWGG 2 cut(s) 320, 540
MvnI CGCG 1 cut(s) 458
MwoI GCNNNNNNNGC 3 cut(s) 23, 138, 896
NciI CCSGG 2 cut(s) 135, 575
NdeI CATATG 1 cut(s) 857
NdeII GATC 2 cut(s) 218, 939
NlaIII CATG 3 cut(s) 84, 283, 301
NlaIV GGNNCC 5 cut(s) 6, 100, 127, 133, 145
NmeAIII GCCGAG 1 cut(s) 330
NmuCI GTSAC 1 cut(s) 481
PaqCI CACCTGC 2 cut(s) 101, 296
PfeI GAWTC 1 cut(s) 869
PkrI GCNGC 5 cut(s) 98, 140, 143, 192, 460
PleI GAGTC 2 cut(s) 407, 870
PmaCI CACGTG 1 cut(s) 471
PmlI CACGTG 1 cut(s) 471
PpsI GAGTC 2 cut(s) 407, 870
Ppu21I YACGTR 1 cut(s) 471
Psp6I CCWGG 2 cut(s) 318, 538
PspCI CACGTG 1 cut(s) 471
PspGI CCWGG 2 cut(s) 318, 538
PspN4I GGNNCC 5 cut(s) 6, 100, 127, 133, 145
PspPI GGNCC 4 cut(s) 131, 322, 333, 353
RseI CAYNNNNRTG 1 cut(s) 296
SaqAI TTAA 1 cut(s) 947
SatI GCNGC 5 cut(s) 97, 139, 142, 191, 459
Sau3AI GATC 2 cut(s) 218, 939
Sau96I GGNCC 4 cut(s) 131, 322, 333, 353
SchI GAGTC 2 cut(s) 407, 871
ScrFI CCNGG 4 cut(s) 135, 320, 540, 575
SduI GDGCHC 2 cut(s) 582, 921
SfaNI GCATC 1 cut(s) 26
SmiMI CAYNNNNRTG 1 cut(s) 296
SmlI CTYRAG 1 cut(s) 581
SmoI CTYRAG 1 cut(s) 581
Sse9I AATT 2 cut(s) 763, 826
SsiI CCGC 3 cut(s) 96, 141, 458
SspMI CTAG 2 cut(s) 548, 566
StyD4I CCNGG 4 cut(s) 133, 318, 538, 573
StyI CCWWGG 1 cut(s) 555
TaaI ACNGT 1 cut(s) 833
TaiI ACGT 1 cut(s) 473
TaqI TCGA 1 cut(s) 327
TasI AATT 2 cut(s) 763, 826
TauI GCSGC 3 cut(s) 99, 144, 461
TfiI GAWTC 1 cut(s) 869
Tru1I TTAA 1 cut(s) 947
Tru9I TTAA 1 cut(s) 947
TscAI CASTG 1 cut(s) 838
TseFI GTSAC 1 cut(s) 481
TseI GCWGC 2 cut(s) 138, 190
Tsp45I GTSAC 1 cut(s) 481
TspDTI ATGAA 2 cut(s) 69, 355
TspGWI ACGGA 3 cut(s) 469, 592, 690
TspRI CASTG 1 cut(s) 838
XmiI GTMKAC 1 cut(s) 531
XspI CTAG 2 cut(s) 548, 566
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.