Rw2G018700

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0014642
Physical Location & Seq
Forward (+)
24453821 .. 24455568
1748 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G018700.1

Sequence Viewer

Length: 945 bp
ATGGAACCACAGCGAGCATCATTAGCTCTTTCACTCATCATCTTCAACCTCCTCCTTACCACCACAACCCAATACCCTTCATGCCAATCCACCTGCGGCTCCCTCCACCTTAGCTACCCCTTCGGCTCCGGCCCCGGCTGCGGCTCCCCAACCTTCCAACCCTACATAACTTGCACAACAACCACCAAGCAGCTCCTTCTGACCACTCACACCGGATCATACCCCATCACCTCCATCTCCTACTCCACCAACACCCTAACCCTAACACCACCCTCCATGTCCACCTGCACCTCCATGCAACCCTGCCCCTCCAACCTGGGCCTCGACTGGGCCAGCCCGTTCCAGCTTGGCCCATCAACTTTCATCCTCCTCTCCTGCCAACCCCCTACCGCCTCTCTCACCCTCAAAGACTCCTCCTCCCCAATCTGCGACCCCTCCTACTCCCACCTCTGCGCGGCCATCTACACTTGTCCCTCCGTCACGGGCCTCAACCTCCCTCTCTTCCCTCCCACCAACACTTGCTGTGTCTACTCCCCTGGAAACCTAGACGCCAAGGGCGAGCTAGACCTCCGGGGGCTCAAGTGTAAGGGATACACCTCCGTCGTGTCGCTCGGGGATTACCCAACCGACCCGAACAAGTGGGAGTATGGGGTGGCGCTGAAGTATAACCACGGAGGCGAGTTGGATGGTGGCATTGTTGAGACGAACTGCAAGAGTTGTGAGATGAGTGATGGCGTTTGTGGTTATGCTACTTCTGATCATTCGTTTCTTTGTGTTTGTAAAAATGGCGACTACAACACCACTTTGGATTGTTCTTCTGATAATTACAGTGGCGTTGGTGTCATATGGAGTCCGGAATCTGGCTCTGATTTACCGGCTTCTTCAGCTTGGAAAATATGGTTTGGGCTCTTGGCTCTGGCTGAGCTGATCATTTTAATAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

314

Amino Acids

33.38

Weight (kDa)

4.73

Isoelectric Point (pI)

40.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 28 - 88 3.5e-12 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 233 - 262 5.9e-07 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014642)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20600
malus_domestica MD01G1032500.v1.1 MD15G1322500.v1.1
prunus_persica Prupe.6G189700_v2.0.a1
pyrus_communis pycom01g05980 pycom15g28310
rosa_chinensis RchiOBHm_Chr2g0113031
rosa_laevigata RLG00000018031
rosa_multiflora Rmu_sc0002351.1_g000105 Rmu_sc0024545.1_g000002
rosa_roxburghii Rroxscaffold_2G00130930
rosa_rugosa Rorug02G0183300
rosa_samantha Rh2AG238500 Rh2BG252300 Rh2CG242500 Rh2DG246500
rosa_wichuraiana Rw2G018700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 101, 293
Acc36I ACCTGC 2 cut(s) 101, 293
AccI GTMKAC 1 cut(s) 528
AccII CGCG 1 cut(s) 455
AccIII TCCGGA 1 cut(s) 853
AciI CCGC 4 cut(s) 96, 141, 390, 455
AclWI GGATC 1 cut(s) 223
AcoI YGGCCR 1 cut(s) 456
AcuI CTGAAG 2 cut(s) 680, 867
AcyI GRCGYC 1 cut(s) 549
AfiI CCNNNNNNNGG 4 cut(s) 140, 328, 454, 860
AgsI TTSAA 1 cut(s) 46
AjnI CCWGG 2 cut(s) 315, 535
AluBI AGCT 8 cut(s) 26, 114, 193, 346, 562, 887, 925, 941
AluI AGCT 8 cut(s) 26, 114, 193, 346, 562, 887, 925, 941
Alw26I GTCTC 1 cut(s) 695
AlwI GGATC 1 cut(s) 223
Ama87I CYCGRG 1 cut(s) 611
Aor13HI TCCGGA 1 cut(s) 853
AoxI GGCC 6 cut(s) 130, 319, 330, 349, 456, 484
ApeKI GCWGC 2 cut(s) 138, 190
AspLEI GCGC 2 cut(s) 455, 658
AspS9I GGNCC 5 cut(s) 131, 319, 330, 350, 484
AsuC2I CCSGG 2 cut(s) 135, 572
AsuHPI GGTGA 2 cut(s) 220, 391
AvaI CYCGRG 1 cut(s) 611
BanII GRGCYC 2 cut(s) 579, 909
BbvI GCAGC 2 cut(s) 125, 202
BccI CCATC 6 cut(s) 233, 242, 361, 467, 680, 725
BciT130I CCWGG 2 cut(s) 317, 537
BciVI GTATCC 1 cut(s) 584
BclI TGATCA 2 cut(s) 757, 927
BcnI CCSGG 2 cut(s) 135, 572
BcoDI GTCTC 1 cut(s) 695
BfaI CTAG 2 cut(s) 545, 563
BfoI RGCGCY 1 cut(s) 659
BfuAI ACCTGC 2 cut(s) 101, 293
BfuI GTATCC 1 cut(s) 584
BisI GCNGC 5 cut(s) 97, 139, 142, 191, 456
BlpI GCTNAGC 1 cut(s) 921
BlsI GCNGC 5 cut(s) 98, 140, 143, 192, 457
Bme1390I CCNGG 4 cut(s) 135, 317, 537, 572
BmeT110I CYCGRG 1 cut(s) 611
BmgT120I GGNCC 5 cut(s) 131, 319, 330, 350, 484
BmiI GGNNCC 5 cut(s) 6, 100, 127, 133, 145
BmrFI CCNGG 4 cut(s) 135, 317, 537, 572
BmrI ACTGGG 1 cut(s) 337
BmsI GCATC 1 cut(s) 26
BmuI ACTGGG 1 cut(s) 337
Bpu10I CCTNAGC 1 cut(s) 110
Bpu1102I GCTNAGC 1 cut(s) 921
BpuEI CTTGAG 1 cut(s) 563
BpuMI CCSGG 2 cut(s) 135, 572
BsaHI GRCGYC 1 cut(s) 549
BsaJI CCNNGG 6 cut(s) 133, 316, 535, 552, 571, 670
BsaWI WCCGGW 2 cut(s) 212, 853
BsaXI ACNNNNNCTCC 2 cut(s) 401, 431
Bsc4I CCNNNNNNNGG 4 cut(s) 140, 328, 454, 860
Bse118I RCCGGY 1 cut(s) 874
Bse1I ACTGG 1 cut(s) 332
BseAI TCCGGA 1 cut(s) 853
BseBI CCWGG 2 cut(s) 317, 537
BseDI CCNNGG 6 cut(s) 133, 316, 535, 552, 571, 670
BseGI GGATG 2 cut(s) 363, 691
BseLI CCNNNNNNNGG 4 cut(s) 140, 328, 454, 860
BseMII CTCAG 1 cut(s) 912
BseNI ACTGG 1 cut(s) 332
BseRI GAGGAG 4 cut(s) 41, 359, 403, 406
BseXI GCAGC 2 cut(s) 125, 202
BsgI GTGCAG 1 cut(s) 271
Bsh1236I CGCG 1 cut(s) 455
BshFI GGCC 6 cut(s) 132, 321, 332, 351, 458, 486
BsiHKCI CYCGRG 1 cut(s) 611
BsiSI CCGG 6 cut(s) 129, 135, 213, 571, 854, 875
BslFI GGGAC 1 cut(s) 456
BslI CCNNNNNNNGG 4 cut(s) 140, 328, 454, 860
BsmAI GTCTC 1 cut(s) 695
BsmBI CGTCTC 1 cut(s) 695
BsmFI GGGAC 1 cut(s) 456
BsnI GGCC 6 cut(s) 132, 321, 332, 351, 458, 486
BsoBI CYCGRG 1 cut(s) 611
Bsp1286I GDGCHC 2 cut(s) 579, 909
Bsp13I TCCGGA 1 cut(s) 853
Bsp143I GATC 3 cut(s) 215, 757, 927
Bsp1720I GCTNAGC 1 cut(s) 921
BspACI CCGC 4 cut(s) 96, 141, 390, 455
BspANI GGCC 6 cut(s) 132, 321, 332, 351, 458, 486
BspCNI CTCAG 1 cut(s) 913
BspEI TCCGGA 1 cut(s) 853
BspFNI CGCG 1 cut(s) 455
BspLI GGNNCC 5 cut(s) 6, 100, 127, 133, 145
BspMI ACCTGC 2 cut(s) 101, 293
BspPI GGATC 1 cut(s) 223
BsrFI RCCGGY 1 cut(s) 874
BsrI ACTGG 1 cut(s) 332
BssAI RCCGGY 1 cut(s) 874
BssECI CCNNGG 6 cut(s) 133, 316, 535, 552, 571, 670
BssMI GATC 3 cut(s) 215, 757, 927
BssNI GRCGYC 1 cut(s) 549
BssT1I CCWWGG 1 cut(s) 552
Bst2UI CCWGG 2 cut(s) 317, 537
Bst4CI ACNGT 1 cut(s) 830
Bst6I CTCTTC 1 cut(s) 506
BstACI GRCGYC 1 cut(s) 549
BstC8I GCNNGC 3 cut(s) 15, 334, 560
BstDEI CTNAG 2 cut(s) 110, 921
BstDSI CCRYGG 1 cut(s) 670
BstF5I GGATG 2 cut(s) 363, 691
BstFNI CGCG 1 cut(s) 455
BstH2I RGCGCY 1 cut(s) 659
BstHHI GCGC 2 cut(s) 455, 658
BstKTI GATC 3 cut(s) 218, 760, 930
BstMAI GTCTC 1 cut(s) 695
BstMBI GATC 3 cut(s) 215, 757, 927
BstMWI GCNNNNNNNGC 3 cut(s) 23, 138, 884
BstNI CCWGG 2 cut(s) 317, 537
BstSCI CCNGG 4 cut(s) 133, 315, 535, 570
BstUI CGCG 1 cut(s) 455
BstV1I GCAGC 2 cut(s) 125, 202
BsuI GTATCC 1 cut(s) 584
BsuRI GGCC 6 cut(s) 132, 321, 332, 351, 458, 486
BtgI CCRYGG 1 cut(s) 670
BtsCI GGATG 2 cut(s) 363, 691
BtsIMutI CAGTG 1 cut(s) 835
BveI ACCTGC 2 cut(s) 101, 293
Cac8I GCNNGC 3 cut(s) 15, 334, 560
CfoI GCGC 2 cut(s) 455, 658
Cfr10I RCCGGY 1 cut(s) 874
Cfr13I GGNCC 5 cut(s) 131, 319, 330, 350, 484
CseI GACGC 1 cut(s) 557
CviAII CATG 3 cut(s) 81, 277, 295
DdeI CTNAG 2 cut(s) 110, 921
DpnI GATC 3 cut(s) 217, 759, 929
DpnII GATC 3 cut(s) 215, 757, 927
EaeI YGGCCR 1 cut(s) 456
Eam1104I CTCTTC 1 cut(s) 506
EarI CTCTTC 1 cut(s) 506
Eco130I CCWWGG 1 cut(s) 552
Eco24I GRGCYC 2 cut(s) 579, 909
Eco57I CTGAAG 2 cut(s) 680, 867
Eco88I CYCGRG 1 cut(s) 611
EcoRII CCWGG 2 cut(s) 315, 535
EcoT14I CCWWGG 1 cut(s) 552
EcoT38I GRGCYC 2 cut(s) 579, 909
ErhI CCWWGG 1 cut(s) 552
Esp3I CGTCTC 1 cut(s) 695
FaeI CATG 3 cut(s) 84, 280, 298
FaqI GGGAC 1 cut(s) 456
FatI CATG 3 cut(s) 80, 276, 294
FauNDI CATATG 1 cut(s) 845
FbaI TGATCA 2 cut(s) 757, 927
FblI GTMKAC 1 cut(s) 528
Fnu4HI GCNGC 5 cut(s) 97, 139, 142, 191, 456
FokI GGATG 2 cut(s) 350, 698
FriOI GRGCYC 2 cut(s) 579, 909
Fsp4HI GCNGC 5 cut(s) 97, 139, 142, 191, 456
FspBI CTAG 2 cut(s) 545, 563
GlaI GCGC 2 cut(s) 454, 657
GluI GCNGC 5 cut(s) 97, 139, 142, 191, 456
HaeII RGCGCY 1 cut(s) 659
HaeIII GGCC 6 cut(s) 132, 321, 332, 351, 458, 486
HapII CCGG 6 cut(s) 129, 135, 213, 571, 854, 875
HgaI GACGC 1 cut(s) 557
HhaI GCGC 2 cut(s) 455, 658
Hin1I GRCGYC 1 cut(s) 549
Hin1II CATG 3 cut(s) 84, 280, 298
Hin6I GCGC 2 cut(s) 453, 656
HinP1I GCGC 2 cut(s) 453, 656
HinfI GANTC 3 cut(s) 410, 850, 857
HpaII CCGG 6 cut(s) 129, 135, 213, 571, 854, 875
HphI GGTGA 2 cut(s) 220, 391
Hpy166II GTNNAC 2 cut(s) 282, 529
Hpy188I TCNGA 4 cut(s) 201, 757, 820, 868
Hpy188III TCNNGA 1 cut(s) 854
Hpy8I GTNNAC 2 cut(s) 282, 529
Hpy99I CGWCG 1 cut(s) 605
HpyAV CCTTC 4 cut(s) 87, 130, 163, 206
HpyCH4III ACNGT 1 cut(s) 830
HpyCH4V TGCA 4 cut(s) 174, 288, 298, 711
HpyF10VI GCNNNNNNNGC 3 cut(s) 23, 138, 884
HpyF3I CTNAG 2 cut(s) 110, 921
Hsp92I GRCGYC 1 cut(s) 549
Hsp92II CATG 3 cut(s) 84, 280, 298
HspAI GCGC 2 cut(s) 453, 656
Kpn2I TCCGGA 1 cut(s) 853
Ksp22I TGATCA 2 cut(s) 757, 927
Kzo9I GATC 3 cut(s) 215, 757, 927
LmnI GCTCC 4 cut(s) 104, 131, 149, 198
Lsp1109I GCAGC 2 cut(s) 125, 202
LweI GCATC 1 cut(s) 26
MaeI CTAG 2 cut(s) 545, 563
MaeIII GTNAC 1 cut(s) 478
MalI GATC 3 cut(s) 217, 759, 929
MboI GATC 3 cut(s) 215, 757, 927
MboII GAAGA 4 cut(s) 34, 493, 807, 873
MhlI GDGCHC 2 cut(s) 579, 909
MluCI AATT 1 cut(s) 823
MlyI GAGTC 2 cut(s) 404, 859
MmeI TCCRAC 3 cut(s) 181, 336, 663
MroI TCCGGA 1 cut(s) 853
MseI TTAA 1 cut(s) 935
MslI CAYNNNNRTG 1 cut(s) 293
MspI CCGG 6 cut(s) 129, 135, 213, 571, 854, 875
MspR9I CCNGG 4 cut(s) 135, 317, 537, 572
MvaI CCWGG 2 cut(s) 317, 537
MvnI CGCG 1 cut(s) 455
MwoI GCNNNNNNNGC 3 cut(s) 23, 138, 884
NciI CCSGG 2 cut(s) 135, 572
NdeI CATATG 1 cut(s) 845
NdeII GATC 3 cut(s) 215, 757, 927
NlaIII CATG 3 cut(s) 84, 280, 298
NlaIV GGNNCC 5 cut(s) 6, 100, 127, 133, 145
NmuCI GTSAC 1 cut(s) 478
PaqCI CACCTGC 2 cut(s) 101, 293
PcsI WCGNNNNNNNCGW 1 cut(s) 555
PfeI GAWTC 1 cut(s) 857
PkrI GCNGC 5 cut(s) 98, 140, 143, 192, 457
PleI GAGTC 2 cut(s) 404, 858
PpsI GAGTC 2 cut(s) 404, 858
Psp6I CCWGG 2 cut(s) 315, 535
PspGI CCWGG 2 cut(s) 315, 535
PspN4I GGNNCC 5 cut(s) 6, 100, 127, 133, 145
PspPI GGNCC 5 cut(s) 131, 319, 330, 350, 484
RseI CAYNNNNRTG 1 cut(s) 293
SaqAI TTAA 1 cut(s) 935
SatI GCNGC 5 cut(s) 97, 139, 142, 191, 456
Sau3AI GATC 3 cut(s) 215, 757, 927
Sau96I GGNCC 5 cut(s) 131, 319, 330, 350, 484
SchI GAGTC 2 cut(s) 404, 859
ScrFI CCNGG 4 cut(s) 135, 317, 537, 572
SduI GDGCHC 2 cut(s) 579, 909
SfaNI GCATC 1 cut(s) 26
SmiMI CAYNNNNRTG 1 cut(s) 293
SmlI CTYRAG 1 cut(s) 578
SmoI CTYRAG 1 cut(s) 578
Sse9I AATT 1 cut(s) 823
SsiI CCGC 4 cut(s) 96, 141, 390, 455
SspMI CTAG 2 cut(s) 545, 563
StyD4I CCNGG 4 cut(s) 133, 315, 535, 570
StyI CCWWGG 1 cut(s) 552
TaaI ACNGT 1 cut(s) 830
TaqI TCGA 1 cut(s) 324
TasI AATT 1 cut(s) 823
TauI GCSGC 3 cut(s) 99, 144, 458
TfiI GAWTC 1 cut(s) 857
Tru1I TTAA 1 cut(s) 935
Tru9I TTAA 1 cut(s) 935
TscAI CASTG 1 cut(s) 835
TseFI GTSAC 1 cut(s) 478
TseI GCWGC 2 cut(s) 138, 190
Tsp45I GTSAC 1 cut(s) 478
TspDTI ATGAA 2 cut(s) 69, 352
TspGWI ACGGA 3 cut(s) 466, 589, 687
TspRI CASTG 1 cut(s) 835
XmiI GTMKAC 1 cut(s) 528
XspI CTAG 2 cut(s) 545, 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.