MD01G1032500.v1.1

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0014642
Physical Location & Seq
Reverse (-)
11324820 .. 11326815
1996 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1032500.v1.1.491

Sequence Viewer

Length: 1077 bp
ATGGTCCACTGCTTTCTTACACCTGTAGTCCTCCTCCTATGCCCATTAAACACAAGCCTACTGCTCTCGCTCCTCTTTGGAAAAGATCATAACATCATCGATCACAAGACAGAGAAAGAGGAGAGAGAGAGAAGCCAAGAGAAAGCTCTAATGGTTGTAGTACCAGCTCTTTCAGTATTCATCCTCCTCAACCTTGCCACAGCCGCCGCCGCAGACTCTCCTGCATGCAACCCTACATGTGGCTCCCTGCAACTCAAATACCCTTTTGGCACAGGCCCTGGTTGTGGCTCCCCAATTTTCCAATCCTACATAACCTGTACATTTACCAATAATCAGCAGCTCCTCCTCCTCACCACCCACACTGGCTTATACCCTATCACTTCAATTTCCTACTCCACCCAAACCATAACCCTAACCCCACCCTCCATGTTCAACTGCACCTCCATGCAACCCTCCCTCTCCAACTTCGGCCTTGACTGGGCCTCCCCATTTCAGCTCGGCCCATCAACCTTCATCCTCCTGTCATGCCAGCCCCCCACCTCTTCCCTCACCCTCAAACCCTCCGGCATGCCCGTCTGTGATCCCTGCTACTCTCAACTCTGTGCTTCTATCTACACATGTCCATCCGTGGTTGGTCTTGGCCTCCCCTTGTTCCCTCCCACAAACACGTGCTGCGTGTATTCACCTGGGAATCTTGATGCTAAGGGTGAGTTGGACCTCCACGGGTTAAGGTGCACATTTACATCCGTTGTGTCGCTCGGAGACTACCCGACGGACCCCGTGCAGTGGGAGTATGGGGTGGCGTTGAAGTACAGTCACGGTGGTTTGGACAGTGGCATTGTCGATACAAAGTGCAAGAGCTGTGAGATGAGCGATGGGGTTTGTGGGTACAGGGTTGATGATCATGATCAGTTCCTTTGTGTGTGTAAGAATGGCTACAACACCTCCTCCGATTGCCTTAATAATTACACTCCAGACTCGAAGCCCCTTTGGGGCTCCGGCGCATCTGATGATTTACCAGTTGCTAATTGGAAAATATGGTCTGCGCTCATGGCTGGCCTGATGATAATGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

359

Amino Acids

38.59

Weight (kDa)

5.0

Isoelectric Point (pI)

41.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 76 - 139 2.1e-11 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 282 - 311 8.9e-06 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014642)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20600
malus_domestica MD01G1032500.v1.1 MD15G1322500.v1.1
prunus_persica Prupe.6G189700_v2.0.a1
pyrus_communis pycom01g05980 pycom15g28310
rosa_chinensis RchiOBHm_Chr2g0113031
rosa_laevigata RLG00000018031
rosa_multiflora Rmu_sc0002351.1_g000105 Rmu_sc0024545.1_g000002
rosa_roxburghii Rroxscaffold_2G00130930
rosa_rugosa Rorug02G0183300
rosa_samantha Rh2AG238500 Rh2BG252300 Rh2CG242500 Rh2DG246500
rosa_wichuraiana Rw2G018700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 204, 207, 210
AclWI GGATC 1 cut(s) 575
AcvI CACGTG 1 cut(s) 669
AfaI GTAC 4 cut(s) 162, 319, 812, 890
AfiI CCNNNNNNNGG 5 cut(s) 239, 284, 478, 786, 992
AflIII ACRYGT 3 cut(s) 236, 617, 666
AgsI TTSAA 3 cut(s) 384, 433, 808
AjnI CCWGG 2 cut(s) 277, 685
AluBI AGCT 5 cut(s) 146, 167, 340, 496, 861
AluI AGCT 5 cut(s) 146, 167, 340, 496, 861
Alw21I GWGCWC 1 cut(s) 737
Alw26I GTCTC 1 cut(s) 756
Alw44I GTGCAC 1 cut(s) 733
AlwI GGATC 1 cut(s) 575
AlwNI CAGNNNCTG 1 cut(s) 278
AoxI GGCC 6 cut(s) 274, 469, 480, 499, 640, 1057
ApaLI GTGCAC 1 cut(s) 733
ApeKI GCWGC 2 cut(s) 337, 672
AspLEI GCGC 2 cut(s) 1004, 1048
AspS9I GGNCC 6 cut(s) 4, 275, 480, 500, 715, 775
AsuHPI GGTGA 4 cut(s) 343, 541, 675, 719
AvaII GGWCC 3 cut(s) 4, 715, 775
BaeGI GKGCMC 1 cut(s) 737
BanII GRGCYC 1 cut(s) 998
BbrPI CACGTG 1 cut(s) 669
Bbv12I GWGCWC 1 cut(s) 737
BbvI GCAGC 2 cut(s) 349, 659
BccI CCATC 3 cut(s) 511, 631, 869
BciT130I CCWGG 2 cut(s) 279, 687
BclI TGATCA 2 cut(s) 901, 907
BcoDI GTCTC 1 cut(s) 756
BfmI CTRYAG 1 cut(s) 24
BisI GCNGC 5 cut(s) 204, 207, 210, 338, 673
BlsI GCNGC 5 cut(s) 205, 208, 211, 339, 674
Bme1390I CCNGG 2 cut(s) 279, 687
Bme18I GGWCC 3 cut(s) 4, 715, 775
BmgT120I GGNCC 6 cut(s) 4, 275, 480, 500, 715, 775
BmiI GGNNCC 4 cut(s) 244, 289, 777, 997
BmrFI CCNGG 2 cut(s) 279, 687
BmrI ACTGGG 1 cut(s) 487
BmsI GCATC 2 cut(s) 688, 1013
BmuI ACTGGG 1 cut(s) 487
BpmI CTGGAG 1 cut(s) 957
Bpu10I CCTNAGC 1 cut(s) 702
Bsa29I ATCGAT 1 cut(s) 99
BsaAI YACGTR 1 cut(s) 669
BsaBI GATNNNNATC 1 cut(s) 906
BsaJI CCNNGG 4 cut(s) 277, 627, 686, 721
BsaXI ACNNNNNCTCC 8 cut(s) 425, 455, 467, 497, 929, 932, 959, 962
Bsc4I CCNNNNNNNGG 5 cut(s) 239, 284, 478, 786, 992
Bse1I ACTGG 3 cut(s) 367, 482, 1019
Bse8I GATNNNNATC 1 cut(s) 906
BseBI CCWGG 2 cut(s) 279, 687
BseCI ATCGAT 1 cut(s) 99
BseDI CCNNGG 4 cut(s) 277, 627, 686, 721
BseGI GGATG 4 cut(s) 180, 513, 623, 743
BseJI GATNNNNATC 1 cut(s) 906
BseLI CCNNNNNNNGG 5 cut(s) 239, 284, 478, 786, 992
BseNI ACTGG 3 cut(s) 367, 482, 1019
BseRI GAGGAG 8 cut(s) 23, 62, 134, 176, 332, 335, 338, 937
BseSI GKGCMC 1 cut(s) 737
BseXI GCAGC 2 cut(s) 349, 659
BsgI GTGCAG 2 cut(s) 421, 803
BshFI GGCC 6 cut(s) 276, 471, 482, 501, 642, 1059
BshVI ATCGAT 1 cut(s) 99
BsiHKAI GWGCWC 1 cut(s) 737
BsiSI CCGG 2 cut(s) 564, 999
BslI CCNNNNNNNGG 5 cut(s) 239, 284, 478, 786, 992
BsmAI GTCTC 1 cut(s) 756
BsnI GGCC 6 cut(s) 276, 471, 482, 501, 642, 1059
Bsp1286I GDGCHC 2 cut(s) 737, 998
Bsp1407I TGTACA 1 cut(s) 317
Bsp143I GATC 5 cut(s) 85, 100, 580, 901, 907
BspACI CCGC 3 cut(s) 204, 207, 210
BspANI GGCC 6 cut(s) 276, 471, 482, 501, 642, 1059
BspDI ATCGAT 1 cut(s) 99
BspHI TCATGA 1 cut(s) 904
BspLI GGNNCC 4 cut(s) 244, 289, 777, 997
BspPI GGATC 1 cut(s) 575
BsrGI TGTACA 1 cut(s) 317
BsrI ACTGG 3 cut(s) 367, 482, 1019
BssECI CCNNGG 4 cut(s) 277, 627, 686, 721
BssMI GATC 5 cut(s) 85, 100, 580, 901, 907
Bst2UI CCWGG 2 cut(s) 279, 687
Bst4CI ACNGT 3 cut(s) 815, 821, 833
Bst6I CTCTTC 1 cut(s) 547
BstAUI TGTACA 1 cut(s) 317
BstBAI YACGTR 1 cut(s) 669
BstC8I GCNNGC 4 cut(s) 226, 530, 569, 1057
BstDEI CTNAG 1 cut(s) 702
BstDSI CCRYGG 2 cut(s) 627, 721
BstF5I GGATG 4 cut(s) 180, 513, 623, 743
BstHHI GCGC 2 cut(s) 1004, 1048
BstKTI GATC 5 cut(s) 88, 103, 583, 904, 910
BstMAI GTCTC 1 cut(s) 756
BstMBI GATC 5 cut(s) 85, 100, 580, 901, 907
BstMWI GCNNNNNNNGC 3 cut(s) 203, 209, 1052
BstNI CCWGG 2 cut(s) 279, 687
BstNSI RCATGY 4 cut(s) 228, 240, 571, 621
BstSCI CCNGG 2 cut(s) 277, 685
BstSFI CTRYAG 1 cut(s) 24
BstSLI GKGCMC 1 cut(s) 737
BstV1I GCAGC 2 cut(s) 349, 659
Bsu15I ATCGAT 1 cut(s) 99
BsuRI GGCC 6 cut(s) 276, 471, 482, 501, 642, 1059
BsuTUI ATCGAT 1 cut(s) 99
BtgI CCRYGG 2 cut(s) 627, 721
BtgZI GCGATG 1 cut(s) 888
BtsCI GGATG 4 cut(s) 180, 513, 623, 743
BtsI GCAGTG 2 cut(s) 7, 791
BtsIMutI CAGTG 4 cut(s) 7, 360, 791, 838
Cac8I GCNNGC 4 cut(s) 226, 530, 569, 1057
CaiI CAGNNNCTG 1 cut(s) 278
CciI TCATGA 1 cut(s) 904
CfoI GCGC 2 cut(s) 1004, 1048
Cfr13I GGNCC 6 cut(s) 4, 275, 480, 500, 715, 775
ClaI ATCGAT 1 cut(s) 99
Csp6I GTAC 4 cut(s) 161, 318, 811, 889
CviAII CATG 9 cut(s) 225, 237, 427, 445, 525, 568, 618, 905, 1051
CviQI GTAC 4 cut(s) 161, 318, 811, 889
DdeI CTNAG 1 cut(s) 702
DpnI GATC 5 cut(s) 87, 102, 582, 903, 909
DpnII GATC 5 cut(s) 85, 100, 580, 901, 907
Eam1104I CTCTTC 1 cut(s) 547
EarI CTCTTC 1 cut(s) 547
Eco24I GRGCYC 1 cut(s) 998
Eco47I GGWCC 3 cut(s) 4, 715, 775
Eco72I CACGTG 1 cut(s) 669
EcoO109I RGGNCCY 1 cut(s) 275
EcoRII CCWGG 2 cut(s) 277, 685
EcoT38I GRGCYC 1 cut(s) 998
FaeI CATG 9 cut(s) 228, 240, 430, 448, 528, 571, 621, 908, 1054
FatI CATG 9 cut(s) 224, 236, 426, 444, 524, 567, 617, 904, 1050
FbaI TGATCA 2 cut(s) 901, 907
Fnu4HI GCNGC 5 cut(s) 204, 207, 210, 338, 673
FokI GGATG 4 cut(s) 167, 500, 610, 730
FriOI GRGCYC 1 cut(s) 998
Fsp4HI GCNGC 5 cut(s) 204, 207, 210, 338, 673
GlaI GCGC 2 cut(s) 1003, 1047
GluI GCNGC 5 cut(s) 204, 207, 210, 338, 673
GsuI CTGGAG 1 cut(s) 957
HaeIII GGCC 6 cut(s) 276, 471, 482, 501, 642, 1059
HapII CCGG 2 cut(s) 564, 999
HhaI GCGC 2 cut(s) 1004, 1048
Hin1II CATG 9 cut(s) 228, 240, 430, 448, 528, 571, 621, 908, 1054
Hin6I GCGC 2 cut(s) 1002, 1046
HinP1I GCGC 2 cut(s) 1002, 1046
HinfI GANTC 3 cut(s) 215, 691, 977
HpaII CCGG 2 cut(s) 564, 999
HphI GGTGA 4 cut(s) 343, 541, 675, 719
Hpy166II GTNNAC 2 cut(s) 7, 735
Hpy188I TCNGA 3 cut(s) 761, 952, 1009
Hpy188III TCNNGA 3 cut(s) 695, 905, 974
Hpy8I GTNNAC 2 cut(s) 7, 735
Hpy99I CGWCG 1 cut(s) 775
HpyAV CCTTC 1 cut(s) 520
HpyCH4III ACNGT 3 cut(s) 815, 821, 833
HpyCH4IV ACGT 1 cut(s) 668
HpyCH4V TGCA 8 cut(s) 224, 228, 250, 438, 448, 735, 784, 855
HpyF10VI GCNNNNNNNGC 3 cut(s) 203, 209, 1052
HpyF3I CTNAG 1 cut(s) 702
HpySE526I ACGT 1 cut(s) 668
Hsp92II CATG 9 cut(s) 228, 240, 430, 448, 528, 571, 621, 908, 1054
HspAI GCGC 2 cut(s) 1002, 1046
Ksp22I TGATCA 2 cut(s) 901, 907
Kzo9I GATC 5 cut(s) 85, 100, 580, 901, 907
LmnI GCTCC 5 cut(s) 75, 248, 293, 345, 1001
Lsp1109I GCAGC 2 cut(s) 349, 659
LweI GCATC 2 cut(s) 688, 1013
MaeII ACGT 1 cut(s) 668
MaeIII GTNAC 1 cut(s) 815
MalI GATC 5 cut(s) 87, 102, 582, 903, 909
MboI GATC 5 cut(s) 85, 100, 580, 901, 907
MboII GAAGA 1 cut(s) 534
MhlI GDGCHC 2 cut(s) 737, 998
MluCI AATT 4 cut(s) 294, 384, 964, 1027
MlyI GAGTC 2 cut(s) 209, 971
MmeI TCCRAC 2 cut(s) 486, 693
MseI TTAA 3 cut(s) 47, 728, 960
MslI CAYNNNNRTG 1 cut(s) 443
MspI CCGG 2 cut(s) 564, 999
MspR9I CCNGG 2 cut(s) 279, 687
MvaI CCWGG 2 cut(s) 279, 687
MwoI GCNNNNNNNGC 3 cut(s) 203, 209, 1052
NdeII GATC 5 cut(s) 85, 100, 580, 901, 907
NlaIII CATG 9 cut(s) 228, 240, 430, 448, 528, 571, 621, 908, 1054
NlaIV GGNNCC 4 cut(s) 244, 289, 777, 997
NmeAIII GCCGAG 1 cut(s) 477
NmuCI GTSAC 1 cut(s) 815
NspI RCATGY 4 cut(s) 228, 240, 571, 621
PaeI GCATGC 2 cut(s) 228, 571
PagI TCATGA 1 cut(s) 904
PciI ACATGT 2 cut(s) 236, 617
PfeI GAWTC 1 cut(s) 691
PkrI GCNGC 5 cut(s) 205, 208, 211, 339, 674
PleI GAGTC 2 cut(s) 209, 971
PmaCI CACGTG 1 cut(s) 669
PmlI CACGTG 1 cut(s) 669
PpsI GAGTC 2 cut(s) 209, 971
Ppu21I YACGTR 1 cut(s) 669
PscI ACATGT 2 cut(s) 236, 617
Psp6I CCWGG 2 cut(s) 277, 685
PspCI CACGTG 1 cut(s) 669
PspGI CCWGG 2 cut(s) 277, 685
PspN4I GGNNCC 4 cut(s) 244, 289, 777, 997
PspPI GGNCC 6 cut(s) 4, 275, 480, 500, 715, 775
PstNI CAGNNNCTG 1 cut(s) 278
RsaI GTAC 4 cut(s) 162, 319, 812, 890
RsaNI GTAC 4 cut(s) 161, 318, 811, 889
RseI CAYNNNNRTG 1 cut(s) 443
SaqAI TTAA 3 cut(s) 47, 728, 960
SatI GCNGC 5 cut(s) 204, 207, 210, 338, 673
Sau3AI GATC 5 cut(s) 85, 100, 580, 901, 907
Sau96I GGNCC 6 cut(s) 4, 275, 480, 500, 715, 775
SchI GAGTC 2 cut(s) 209, 971
ScrFI CCNGG 2 cut(s) 279, 687
SduI GDGCHC 2 cut(s) 737, 998
SfaNI GCATC 2 cut(s) 688, 1013
SfcI CTRYAG 1 cut(s) 24
SinI GGWCC 3 cut(s) 4, 715, 775
SmiMI CAYNNNNRTG 1 cut(s) 443
SphI GCATGC 2 cut(s) 228, 571
Sse9I AATT 4 cut(s) 294, 384, 964, 1027
SsiI CCGC 3 cut(s) 204, 207, 210
StyD4I CCNGG 2 cut(s) 277, 685
TaaI ACNGT 3 cut(s) 815, 821, 833
TaiI ACGT 1 cut(s) 671
TaqI TCGA 3 cut(s) 99, 843, 980
TasI AATT 4 cut(s) 294, 384, 964, 1027
TatI WGTACW 2 cut(s) 317, 810
TauI GCSGC 3 cut(s) 206, 209, 212
TfiI GAWTC 1 cut(s) 691
Tru1I TTAA 3 cut(s) 47, 728, 960
Tru9I TTAA 3 cut(s) 47, 728, 960
TscAI CASTG 4 cut(s) 14, 367, 791, 838
TseFI GTSAC 1 cut(s) 815
TseI GCWGC 2 cut(s) 337, 672
Tsp45I GTSAC 1 cut(s) 815
TspDTI ATGAA 2 cut(s) 169, 502
TspGWI ACGGA 3 cut(s) 616, 736, 788
TspRI CASTG 4 cut(s) 14, 367, 791, 838
VneI GTGCAC 1 cut(s) 733
VpaK11BI GGWCC 3 cut(s) 4, 715, 775
XceI RCATGY 4 cut(s) 228, 240, 571, 621
XcmI CCANNNNNNNNNTGG 1 cut(s) 1026
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.