RLG00000018031

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0014642
Physical Location & Seq
Forward (+)
24629254 .. 24630866
1613 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018031

Sequence Viewer

Length: 954 bp
ATGGAACCACAGCGAGCCTCATTAGCTCTTTCACTCATCATCTTCAACCTCCTCCTCACCACCACAACCCAATACCCTTCATGCCAATCCACCTGCGCCTCCCACCAACTTAGCTACCCTTTCGGCTCCGGCCCCGGCTGCGGCTCCCCAACCTTCCAACCCTACATAACTTGCACAACTAACACCAAACAGCTCCTTCTGACCACTCACACCGGATCATACCCCATCACCTCCATCTCCTACTCCACCAACACCCTGACCCTAACACCACCCTCCATGTCCACCTGCACCTCCATGCAACCCTGCCCCTCCAACCTGGGCCTCGACTGGGCCAGCCCGTTCCAGCTCGGCCCATCAACTTTCATCCTCCTCTCCTGCCAACCACCGACCTCCTCTCTCACCCTCAAAGACTCCTCCTCCCCAATCTGCGACCCCTCCTACTCCCACCTCTGCGCCGCCATCTACACGTGTCCCTCAGTCACCAGCCTCAACCTCCCTCTCTTCCCTCCCACCAACACTTGCTGTGTCTACTCCCCAGGAAACCTCGACGCCAAGGGCGAGCTAGACCTCCGGGGCCTCAAGTGCAAGGGATACACCTCCGTCGTGTCGCTCGGGGATTACCCAACCGACCCGAACAAGTGGGAGTATGGGGTGGCGCTGAAGTATAACCACGGAGGCGGGTTGGATGGTGGCATTGTGGAGACGAAGTGCAACAGTTGTGAGATGAGTGGTGGCGTTTGTGGTTATGCTACTTCTGATAATTCGTTTCTCTGTGTTTGTAAAAATGGCGACTACAACACCACTTCGGATTGTTCGTCTGATAATTACAGCGGTGTTCAAGGACAAGGTGAAATGTGGAGTCCGGAATCTGGCTCTGATTTACCAGCTTCTTCAGCTTGGAAAATACGGTTTGGGCTCTTGGCTCTGGCTGACCTGGTCATTTTAACAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

318

Amino Acids

33.58

Weight (kDa)

4.81

Isoelectric Point (pI)

41.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GUB_WAK_bind PF13947 28 - 88 1.7e-11 Wall-associated receptor kinase galacturonan-binding
WAK_assoc PF14380 234 - 262 9.7e-07 Wall-associated receptor kinase C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014642)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20600
malus_domestica MD01G1032500.v1.1 MD15G1322500.v1.1
prunus_persica Prupe.6G189700_v2.0.a1
pyrus_communis pycom01g05980 pycom15g28310
rosa_chinensis RchiOBHm_Chr2g0113031
rosa_laevigata RLG00000018031
rosa_multiflora Rmu_sc0002351.1_g000105 Rmu_sc0024545.1_g000002
rosa_roxburghii Rroxscaffold_2G00130930
rosa_rugosa Rorug02G0183300
rosa_samantha Rh2AG238500 Rh2BG252300 Rh2CG242500 Rh2DG246500
rosa_wichuraiana Rw2G018700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 101, 293
Acc36I ACCTGC 2 cut(s) 101, 293
AccI GTMKAC 1 cut(s) 528
AccIII TCCGGA 1 cut(s) 862
AciI CCGC 4 cut(s) 141, 456, 678, 831
AclWI GGATC 1 cut(s) 223
AcuI CTGAAG 2 cut(s) 680, 876
AcvI CACGTG 1 cut(s) 468
AcyI GRCGYC 1 cut(s) 549
AfiI CCNNNNNNNGG 3 cut(s) 140, 328, 869
AflIII ACRYGT 2 cut(s) 465, 467
AgsI TTSAA 2 cut(s) 46, 839
AjnI CCWGG 3 cut(s) 315, 535, 933
AluBI AGCT 8 cut(s) 26, 114, 193, 346, 562, 887, 896, 950
AluI AGCT 8 cut(s) 26, 114, 193, 346, 562, 887, 896, 950
Alw26I GTCTC 1 cut(s) 695
AlwI GGATC 1 cut(s) 223
Ama87I CYCGRG 1 cut(s) 611
Aor13HI TCCGGA 1 cut(s) 862
AoxI GGCC 5 cut(s) 130, 319, 330, 349, 574
ApeKI GCWGC 1 cut(s) 138
AspLEI GCGC 3 cut(s) 98, 455, 658
AspS9I GGNCC 5 cut(s) 131, 319, 330, 350, 574
AsuC2I CCSGG 2 cut(s) 135, 572
AsuHPI GGTGA 5 cut(s) 49, 220, 391, 472, 860
AvaI CYCGRG 1 cut(s) 611
BanII GRGCYC 1 cut(s) 918
BbrPI CACGTG 1 cut(s) 468
BbvI GCAGC 1 cut(s) 125
BccI CCATC 5 cut(s) 233, 242, 361, 467, 680
BciT130I CCWGG 3 cut(s) 317, 537, 935
BciVI GTATCC 1 cut(s) 584
BcnI CCSGG 2 cut(s) 135, 572
BcoDI GTCTC 1 cut(s) 695
BfaI CTAG 1 cut(s) 563
BfoI RGCGCY 1 cut(s) 659
BfuAI ACCTGC 2 cut(s) 101, 293
BfuI GTATCC 1 cut(s) 584
BisI GCNGC 3 cut(s) 139, 142, 456
BlsI GCNGC 3 cut(s) 140, 143, 457
Bme1390I CCNGG 5 cut(s) 135, 317, 537, 572, 935
BmeT110I CYCGRG 1 cut(s) 611
BmgT120I GGNCC 5 cut(s) 131, 319, 330, 350, 574
BmiI GGNNCC 5 cut(s) 6, 127, 133, 145, 575
BmrFI CCNGG 5 cut(s) 135, 317, 537, 572, 935
BmrI ACTGGG 1 cut(s) 337
BmuI ACTGGG 1 cut(s) 337
BpuEI CTTGAG 1 cut(s) 563
BpuMI CCSGG 2 cut(s) 135, 572
BsaAI YACGTR 1 cut(s) 468
BsaHI GRCGYC 1 cut(s) 549
BsaJI CCNNGG 6 cut(s) 133, 316, 535, 552, 571, 670
BsaWI WCCGGW 2 cut(s) 212, 862
BsaXI ACNNNNNCTCC 4 cut(s) 401, 431, 692, 722
Bsc4I CCNNNNNNNGG 3 cut(s) 140, 328, 869
Bse1I ACTGG 1 cut(s) 332
BseAI TCCGGA 1 cut(s) 862
BseBI CCWGG 3 cut(s) 317, 537, 935
BseDI CCNNGG 6 cut(s) 133, 316, 535, 552, 571, 670
BseGI GGATG 2 cut(s) 363, 691
BseLI CCNNNNNNNGG 3 cut(s) 140, 328, 869
BseMII CTCAG 1 cut(s) 489
BseNI ACTGG 1 cut(s) 332
BseRI GAGGAG 6 cut(s) 41, 44, 359, 382, 403, 406
BseXI GCAGC 1 cut(s) 125
BsgI GTGCAG 1 cut(s) 271
BshFI GGCC 5 cut(s) 132, 321, 332, 351, 576
BsiHKCI CYCGRG 1 cut(s) 611
BsiSI CCGG 5 cut(s) 129, 135, 213, 571, 863
BslFI GGGAC 1 cut(s) 456
BslI CCNNNNNNNGG 3 cut(s) 140, 328, 869
BsmAI GTCTC 1 cut(s) 695
BsmBI CGTCTC 1 cut(s) 695
BsmFI GGGAC 1 cut(s) 456
BsnI GGCC 5 cut(s) 132, 321, 332, 351, 576
BsoBI CYCGRG 1 cut(s) 611
Bsp1286I GDGCHC 1 cut(s) 918
Bsp13I TCCGGA 1 cut(s) 862
Bsp143I GATC 1 cut(s) 215
BspACI CCGC 4 cut(s) 141, 456, 678, 831
BspANI GGCC 5 cut(s) 132, 321, 332, 351, 576
BspCNI CTCAG 1 cut(s) 488
BspEI TCCGGA 1 cut(s) 862
BspLI GGNNCC 5 cut(s) 6, 127, 133, 145, 575
BspMI ACCTGC 2 cut(s) 101, 293
BspPI GGATC 1 cut(s) 223
BsrI ACTGG 1 cut(s) 332
BssECI CCNNGG 6 cut(s) 133, 316, 535, 552, 571, 670
BssMI GATC 1 cut(s) 215
BssNI GRCGYC 1 cut(s) 549
BssT1I CCWWGG 1 cut(s) 552
Bst2UI CCWGG 3 cut(s) 317, 537, 935
Bst4CI ACNGT 2 cut(s) 716, 909
Bst6I CTCTTC 1 cut(s) 506
BstACI GRCGYC 1 cut(s) 549
BstBAI YACGTR 1 cut(s) 468
BstC8I GCNNGC 3 cut(s) 15, 334, 560
BstDEI CTNAG 2 cut(s) 110, 475
BstDSI CCRYGG 1 cut(s) 670
BstF5I GGATG 2 cut(s) 363, 691
BstH2I RGCGCY 1 cut(s) 659
BstHHI GCGC 3 cut(s) 98, 455, 658
BstKTI GATC 1 cut(s) 218
BstMAI GTCTC 1 cut(s) 695
BstMBI GATC 1 cut(s) 215
BstMWI GCNNNNNNNGC 4 cut(s) 23, 138, 582, 893
BstNI CCWGG 3 cut(s) 317, 537, 935
BstSCI CCNGG 5 cut(s) 133, 315, 535, 570, 933
BstV1I GCAGC 1 cut(s) 125
BsuI GTATCC 1 cut(s) 584
BsuRI GGCC 5 cut(s) 132, 321, 332, 351, 576
BtgI CCRYGG 1 cut(s) 670
BtsCI GGATG 2 cut(s) 363, 691
BveI ACCTGC 2 cut(s) 101, 293
Cac8I GCNNGC 3 cut(s) 15, 334, 560
CfoI GCGC 3 cut(s) 98, 455, 658
Cfr13I GGNCC 5 cut(s) 131, 319, 330, 350, 574
CseI GACGC 1 cut(s) 557
CsiI ACCWGGT 1 cut(s) 933
CviAII CATG 3 cut(s) 81, 277, 295
DdeI CTNAG 2 cut(s) 110, 475
DpnI GATC 1 cut(s) 217
DpnII GATC 1 cut(s) 215
Eam1104I CTCTTC 1 cut(s) 506
EarI CTCTTC 1 cut(s) 506
Eco130I CCWWGG 1 cut(s) 552
Eco24I GRGCYC 1 cut(s) 918
Eco57I CTGAAG 2 cut(s) 680, 876
Eco72I CACGTG 1 cut(s) 468
Eco88I CYCGRG 1 cut(s) 611
EcoO109I RGGNCCY 1 cut(s) 574
EcoRII CCWGG 3 cut(s) 315, 535, 933
EcoT14I CCWWGG 1 cut(s) 552
EcoT38I GRGCYC 1 cut(s) 918
ErhI CCWWGG 1 cut(s) 552
Esp3I CGTCTC 1 cut(s) 695
FaeI CATG 3 cut(s) 84, 280, 298
FaiI YATR 8 cut(s) 82, 167, 220, 278, 296, 648, 666, 747
FaqI GGGAC 1 cut(s) 456
FatI CATG 3 cut(s) 80, 276, 294
FauI CCCGC 1 cut(s) 671
FblI GTMKAC 1 cut(s) 528
Fnu4HI GCNGC 3 cut(s) 139, 142, 456
FokI GGATG 2 cut(s) 350, 698
FriOI GRGCYC 1 cut(s) 918
Fsp4HI GCNGC 3 cut(s) 139, 142, 456
FspBI CTAG 1 cut(s) 563
GlaI GCGC 3 cut(s) 97, 454, 657
GluI GCNGC 3 cut(s) 139, 142, 456
HaeII RGCGCY 1 cut(s) 659
HaeIII GGCC 5 cut(s) 132, 321, 332, 351, 576
HapII CCGG 5 cut(s) 129, 135, 213, 571, 863
HgaI GACGC 1 cut(s) 557
HhaI GCGC 3 cut(s) 98, 455, 658
Hin1I GRCGYC 1 cut(s) 549
Hin1II CATG 3 cut(s) 84, 280, 298
Hin6I GCGC 3 cut(s) 96, 453, 656
HinP1I GCGC 3 cut(s) 96, 453, 656
HinfI GANTC 3 cut(s) 410, 859, 866
HpaII CCGG 5 cut(s) 129, 135, 213, 571, 863
HphI GGTGA 5 cut(s) 49, 220, 391, 472, 860
Hpy166II GTNNAC 2 cut(s) 282, 529
Hpy188I TCNGA 5 cut(s) 201, 757, 808, 820, 877
Hpy188III TCNNGA 1 cut(s) 863
Hpy8I GTNNAC 2 cut(s) 282, 529
Hpy99I CGWCG 2 cut(s) 551, 605
HpyAV CCTTC 3 cut(s) 87, 163, 206
HpyCH4III ACNGT 2 cut(s) 716, 909
HpyCH4IV ACGT 1 cut(s) 467
HpyCH4V TGCA 5 cut(s) 174, 288, 298, 585, 711
HpyF10VI GCNNNNNNNGC 4 cut(s) 23, 138, 582, 893
HpyF3I CTNAG 2 cut(s) 110, 475
HpySE526I ACGT 1 cut(s) 467
Hsp92I GRCGYC 1 cut(s) 549
Hsp92II CATG 3 cut(s) 84, 280, 298
HspAI GCGC 3 cut(s) 96, 453, 656
Kpn2I TCCGGA 1 cut(s) 862
Kzo9I GATC 1 cut(s) 215
LmnI GCTCC 3 cut(s) 131, 149, 198
Lsp1109I GCAGC 1 cut(s) 125
MabI ACCWGGT 1 cut(s) 933
MaeI CTAG 1 cut(s) 563
MaeII ACGT 1 cut(s) 467
MaeIII GTNAC 1 cut(s) 478
MalI GATC 1 cut(s) 217
MboI GATC 1 cut(s) 215
MboII GAAGA 3 cut(s) 34, 493, 882
MhlI GDGCHC 1 cut(s) 918
MluCI AATT 2 cut(s) 760, 823
MlyI GAGTC 2 cut(s) 404, 868
MmeI TCCRAC 3 cut(s) 181, 336, 663
MroI TCCGGA 1 cut(s) 862
MseI TTAA 1 cut(s) 944
MslI CAYNNNNRTG 1 cut(s) 293
MspA1I CMGCKG 1 cut(s) 831
MspI CCGG 5 cut(s) 129, 135, 213, 571, 863
MspR9I CCNGG 5 cut(s) 135, 317, 537, 572, 935
MvaI CCWGG 3 cut(s) 317, 537, 935
MwoI GCNNNNNNNGC 4 cut(s) 23, 138, 582, 893
NciI CCSGG 2 cut(s) 135, 572
NdeII GATC 1 cut(s) 215
NlaIII CATG 3 cut(s) 84, 280, 298
NlaIV GGNNCC 5 cut(s) 6, 127, 133, 145, 575
NmeAIII GCCGAG 1 cut(s) 327
NmuCI GTSAC 1 cut(s) 478
PaqCI CACCTGC 2 cut(s) 101, 293
PcsI WCGNNNNNNNCGW 2 cut(s) 555, 812
PfeI GAWTC 1 cut(s) 866
PflFI GACNNNGTC 1 cut(s) 935
PkrI GCNGC 3 cut(s) 140, 143, 457
PleI GAGTC 2 cut(s) 404, 867
PmaCI CACGTG 1 cut(s) 468
PmlI CACGTG 1 cut(s) 468
PpsI GAGTC 2 cut(s) 404, 867
Ppu21I YACGTR 1 cut(s) 468
Psp6I CCWGG 3 cut(s) 315, 535, 933
PspCI CACGTG 1 cut(s) 468
PspGI CCWGG 3 cut(s) 315, 535, 933
PspN4I GGNNCC 5 cut(s) 6, 127, 133, 145, 575
PspPI GGNCC 5 cut(s) 131, 319, 330, 350, 574
PsyI GACNNNGTC 1 cut(s) 935
RseI CAYNNNNRTG 1 cut(s) 293
SaqAI TTAA 1 cut(s) 944
SatI GCNGC 3 cut(s) 139, 142, 456
Sau3AI GATC 1 cut(s) 215
Sau96I GGNCC 5 cut(s) 131, 319, 330, 350, 574
SchI GAGTC 2 cut(s) 404, 868
ScrFI CCNGG 5 cut(s) 135, 317, 537, 572, 935
SduI GDGCHC 1 cut(s) 918
SexAI ACCWGGT 1 cut(s) 933
SmiMI CAYNNNNRTG 1 cut(s) 293
SmlI CTYRAG 1 cut(s) 578
SmoI CTYRAG 1 cut(s) 578
Sse9I AATT 2 cut(s) 760, 823
SsiI CCGC 4 cut(s) 141, 456, 678, 831
SspMI CTAG 1 cut(s) 563
StyD4I CCNGG 5 cut(s) 133, 315, 535, 570, 933
StyI CCWWGG 1 cut(s) 552
TaaI ACNGT 2 cut(s) 716, 909
TaiI ACGT 1 cut(s) 470
TaqI TCGA 2 cut(s) 324, 546
TasI AATT 2 cut(s) 760, 823
TauI GCSGC 2 cut(s) 144, 458
TfiI GAWTC 1 cut(s) 866
Tru1I TTAA 1 cut(s) 944
Tru9I TTAA 1 cut(s) 944
TseFI GTSAC 1 cut(s) 478
TseI GCWGC 1 cut(s) 138
Tsp45I GTSAC 1 cut(s) 478
TspDTI ATGAA 2 cut(s) 69, 352
TspGWI ACGGA 2 cut(s) 589, 687
Tth111I GACNNNGTC 1 cut(s) 935
XmiI GTMKAC 1 cut(s) 528
XspI CTAG 1 cut(s) 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.