pycom15g28310

wall-associated receptor kinase-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0014642
Physical Location & Seq
Forward (+)
24116085 .. 24117531
1447 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g28310.2

Sequence Viewer

Length: 963 bp
ATGGTTGGAGTACCAGCTCTTTCAATATTCATCCTCCTCCTCCTCCTCCTCTTCAACCACCGCTACATCACCACAGCCTTCGCCACAGACTCGCCTGCTTGCAAGCCCACATGTGGATCCCTGCAACTGAGATACCCTTTTGGCACAGGCCCTGGCTGTGGCTCACCAATTTTCCAACCCTACATAACCTGCGCATTTATCAATAATCAGCAGCAGCTCCTCCTCCTCACCACCCACACAGGCTCATACCCTATCACTTCCATTTCCTACGCCACCCAAACCCTAATCCTAAGCCCACCCTCCATGTCCAACTGCACCTCCATGCAACCTTCTTCCTCCAACTTCGGCCTTGACTGGGCCTCCCCATTTCAGCTCGGCCCATCAACTTTCATCCTCTTGTCCTGCCAGACCCCCACCTCTTCCCTCACCCTCAAACCCTCCGGCATCCCCGTCTGTGATCCCTCCTACTCTTACCTTTGTGCTTCTATATATACGTGTCCATCCGTCGTAGGTCTTGGCCTCCCCTTGTTCCCCCCAACAAACACATGCTGTGTGTATTCACCAGGGAATCTTGATGCTAAGGGTGAGTTGGACCTCCACGGGTTAATGTGTGCCTCATTTACATCCGTGGTGTCTCTCGGAGACTACCCGACGGACCCTGTGCGGTGGGAGTATGGGGTGGCGTTGAAGTACAGTCACGGTGGTTTGGACAGTGGCATTGTGGATACAAAGTGCAAGAGCTGTGAGATGAGTGATGGGGTTTGTGGGTATAGGGTTGATGATCAGGATCAGTTTCTTTGTGTGTGTAAGAATGGTTACAACACCTCATCAGATTGCCATAATAATTACACTCCGGATTCGGAACTGCTTTGGGGCTCCGGCGCCTGTGATGATCATTTACCAGTTGCTATTTGGAAAATGTGGTCTGCGCTTGTGGCTGGCTTGATGATCATAATGGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GUB_WAK_bind WAK_assoc

Protein Analysis

321

Amino Acids

34.37

Weight (kDa)

4.86

Isoelectric Point (pI)

44.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014642)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g20600
malus_domestica MD01G1032500.v1.1 MD15G1322500.v1.1
prunus_persica Prupe.6G189700_v2.0.a1
pyrus_communis pycom01g05980 pycom15g28310
rosa_chinensis RchiOBHm_Chr2g0113031
rosa_laevigata RLG00000018031
rosa_multiflora Rmu_sc0002351.1_g000105 Rmu_sc0024545.1_g000002
rosa_roxburghii Rroxscaffold_2G00130930
rosa_rugosa Rorug02G0183300
rosa_samantha Rh2AG238500 Rh2BG252300 Rh2CG242500 Rh2DG246500
rosa_wichuraiana Rw2G018700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 193
Acc36I ACCTGC 1 cut(s) 197
AccB1I GGYRCC 1 cut(s) 881
AccIII TCCGGA 1 cut(s) 853
AciI CCGC 2 cut(s) 61, 664
AclWI GGATC 4 cut(s) 111, 124, 452, 795
AcyI GRCGYC 1 cut(s) 882
AfaI GTAC 2 cut(s) 12, 692
AfiI CCNNNNNNNGG 3 cut(s) 113, 158, 355
AflIII ACRYGT 2 cut(s) 110, 494
AgsI TTSAA 3 cut(s) 24, 55, 688
AjnI CCWGG 2 cut(s) 151, 562
AluBI AGCT 4 cut(s) 17, 217, 373, 741
AluI AGCT 4 cut(s) 17, 217, 373, 741
Alw26I GTCTC 2 cut(s) 636, 639
AlwI GGATC 4 cut(s) 111, 124, 452, 795
AlwNI CAGNNNCTG 1 cut(s) 152
Aor13HI TCCGGA 1 cut(s) 853
AoxI GGCC 5 cut(s) 148, 346, 357, 376, 517
ApeKI GCWGC 2 cut(s) 211, 214
AspLEI GCGC 3 cut(s) 194, 884, 931
AspS9I GGNCC 5 cut(s) 149, 357, 377, 592, 655
AsuHPI GGTGA 6 cut(s) 61, 156, 220, 418, 552, 596
AvaII GGWCC 2 cut(s) 592, 655
BamHI GGATCC 1 cut(s) 116
BanI GGYRCC 1 cut(s) 881
BanII GRGCYC 1 cut(s) 878
BbvI GCAGC 2 cut(s) 223, 226
BccI CCATC 3 cut(s) 388, 508, 749
BciT130I CCWGG 2 cut(s) 153, 564
BciVI GTATCC 1 cut(s) 718
BclI TGATCA 3 cut(s) 781, 892, 948
BcoDI GTCTC 2 cut(s) 636, 639
BfoI RGCGCY 1 cut(s) 885
BfuAI ACCTGC 1 cut(s) 197
BfuI GTATCC 1 cut(s) 718
BisI GCNGC 2 cut(s) 212, 215
BlsI GCNGC 2 cut(s) 213, 216
Bme1390I CCNGG 2 cut(s) 153, 564
Bme18I GGWCC 2 cut(s) 592, 655
BmgT120I GGNCC 5 cut(s) 149, 357, 377, 592, 655
BmiI GGNNCC 4 cut(s) 118, 657, 877, 883
BmrFI CCNGG 2 cut(s) 153, 564
BmrI ACTGGG 1 cut(s) 364
BmsI GCATC 2 cut(s) 453, 565
BmuI ACTGGG 1 cut(s) 364
Bpu10I CCTNAGC 2 cut(s) 290, 579
BsaAI YACGTR 1 cut(s) 495
BsaBI GATNNNNATC 1 cut(s) 786
BsaHI GRCGYC 1 cut(s) 882
BsaJI CCNNGG 4 cut(s) 151, 563, 598, 627
BsaWI WCCGGW 1 cut(s) 853
BsaXI ACNNNNNCTCC 4 cut(s) 302, 332, 344, 374
Bsc4I CCNNNNNNNGG 3 cut(s) 113, 158, 355
Bse1I ACTGG 2 cut(s) 359, 902
Bse8I GATNNNNATC 1 cut(s) 786
BseAI TCCGGA 1 cut(s) 853
BseBI CCWGG 2 cut(s) 153, 564
BseDI CCNNGG 4 cut(s) 151, 563, 598, 627
BseGI GGATG 5 cut(s) 30, 390, 444, 500, 623
BseJI GATNNNNATC 1 cut(s) 786
BseLI CCNNNNNNNGG 3 cut(s) 113, 158, 355
BseMII CTCAG 1 cut(s) 119
BseNI ACTGG 2 cut(s) 359, 902
BseRI GAGGAG 8 cut(s) 26, 29, 32, 35, 38, 209, 212, 215
BseXI GCAGC 2 cut(s) 223, 226
BsgI GTGCAG 1 cut(s) 298
BshFI GGCC 5 cut(s) 150, 348, 359, 378, 519
BshNI GGYRCC 1 cut(s) 881
BsiSI CCGG 3 cut(s) 441, 854, 879
BslI CCNNNNNNNGG 3 cut(s) 113, 158, 355
BsmAI GTCTC 2 cut(s) 636, 639
BsnI GGCC 5 cut(s) 150, 348, 359, 378, 519
Bsp1286I GDGCHC 1 cut(s) 878
Bsp13I TCCGGA 1 cut(s) 853
Bsp143I GATC 6 cut(s) 116, 457, 781, 787, 892, 948
BspACI CCGC 2 cut(s) 61, 664
BspANI GGCC 5 cut(s) 150, 348, 359, 378, 519
BspCNI CTCAG 1 cut(s) 120
BspEI TCCGGA 1 cut(s) 853
BspLI GGNNCC 4 cut(s) 118, 657, 877, 883
BspMI ACCTGC 1 cut(s) 197
BspPI GGATC 4 cut(s) 111, 124, 452, 795
BspT107I GGYRCC 1 cut(s) 881
BsrI ACTGG 2 cut(s) 359, 902
BssECI CCNNGG 4 cut(s) 151, 563, 598, 627
BssMI GATC 6 cut(s) 116, 457, 781, 787, 892, 948
BssNI GRCGYC 1 cut(s) 882
Bst2UI CCWGG 2 cut(s) 153, 564
Bst4CI ACNGT 3 cut(s) 695, 701, 713
Bst6I CTCTTC 2 cut(s) 56, 424
BstACI GRCGYC 1 cut(s) 882
BstBAI YACGTR 1 cut(s) 495
BstC8I GCNNGC 4 cut(s) 96, 100, 104, 940
BstDEI CTNAG 3 cut(s) 128, 290, 579
BstDSI CCRYGG 2 cut(s) 598, 627
BstF5I GGATG 5 cut(s) 30, 390, 444, 500, 623
BstH2I RGCGCY 1 cut(s) 885
BstHHI GCGC 3 cut(s) 194, 884, 931
BstKTI GATC 6 cut(s) 119, 460, 784, 790, 895, 951
BstMAI GTCTC 2 cut(s) 636, 639
BstMBI GATC 6 cut(s) 116, 457, 781, 787, 892, 948
BstMWI GCNNNNNNNGC 1 cut(s) 935
BstNI CCWGG 2 cut(s) 153, 564
BstNSI RCATGY 2 cut(s) 114, 549
BstSCI CCNGG 2 cut(s) 151, 562
BstV1I GCAGC 2 cut(s) 223, 226
BstX2I RGATCY 1 cut(s) 116
BstYI RGATCY 1 cut(s) 116
BsuI GTATCC 1 cut(s) 718
BsuRI GGCC 5 cut(s) 150, 348, 359, 378, 519
BtgI CCRYGG 2 cut(s) 598, 627
BtsCI GGATG 5 cut(s) 30, 390, 444, 500, 623
BtsIMutI CAGTG 1 cut(s) 718
BveI ACCTGC 1 cut(s) 197
Cac8I GCNNGC 4 cut(s) 96, 100, 104, 940
CaiI CAGNNNCTG 1 cut(s) 152
CfoI GCGC 3 cut(s) 194, 884, 931
Cfr13I GGNCC 5 cut(s) 149, 357, 377, 592, 655
Csp6I GTAC 2 cut(s) 11, 691
CviAII CATG 4 cut(s) 111, 304, 322, 546
CviQI GTAC 2 cut(s) 11, 691
DdeI CTNAG 3 cut(s) 128, 290, 579
DinI GGCGCC 1 cut(s) 883
DpnI GATC 6 cut(s) 118, 459, 783, 789, 894, 950
DpnII GATC 6 cut(s) 116, 457, 781, 787, 892, 948
Eam1104I CTCTTC 2 cut(s) 56, 424
EarI CTCTTC 2 cut(s) 56, 424
Eco24I GRGCYC 1 cut(s) 878
Eco47I GGWCC 2 cut(s) 592, 655
EcoO109I RGGNCCY 1 cut(s) 149
EcoRII CCWGG 2 cut(s) 151, 562
EcoT38I GRGCYC 1 cut(s) 878
EgeI GGCGCC 1 cut(s) 883
EheI GGCGCC 1 cut(s) 883
FaeI CATG 4 cut(s) 114, 307, 325, 549
FatI CATG 4 cut(s) 110, 303, 321, 545
FbaI TGATCA 3 cut(s) 781, 892, 948
Fnu4HI GCNGC 2 cut(s) 212, 215
FokI GGATG 5 cut(s) 17, 377, 431, 487, 610
FriOI GRGCYC 1 cut(s) 878
Fsp4HI GCNGC 2 cut(s) 212, 215
FspI TGCGCA 1 cut(s) 193
GlaI GCGC 3 cut(s) 193, 883, 930
GluI GCNGC 2 cut(s) 212, 215
HaeII RGCGCY 1 cut(s) 885
HaeIII GGCC 5 cut(s) 150, 348, 359, 378, 519
HapII CCGG 3 cut(s) 441, 854, 879
HhaI GCGC 3 cut(s) 194, 884, 931
Hin1I GRCGYC 1 cut(s) 882
Hin1II CATG 4 cut(s) 114, 307, 325, 549
Hin6I GCGC 3 cut(s) 192, 882, 929
HinP1I GCGC 3 cut(s) 192, 882, 929
HinfI GANTC 3 cut(s) 89, 568, 857
HpaII CCGG 3 cut(s) 441, 854, 879
HphI GGTGA 6 cut(s) 61, 156, 220, 418, 552, 596
Hpy188I TCNGA 3 cut(s) 641, 832, 862
Hpy188III TCNNGA 3 cut(s) 572, 785, 854
Hpy99I CGWCG 2 cut(s) 509, 655
HpyAV CCTTC 2 cut(s) 88, 339
HpyCH4III ACNGT 3 cut(s) 695, 701, 713
HpyCH4IV ACGT 1 cut(s) 494
HpyCH4V TGCA 5 cut(s) 102, 124, 315, 325, 735
HpyF10VI GCNNNNNNNGC 1 cut(s) 935
HpyF3I CTNAG 3 cut(s) 128, 290, 579
HpySE526I ACGT 1 cut(s) 494
Hsp92I GRCGYC 1 cut(s) 882
Hsp92II CATG 4 cut(s) 114, 307, 325, 549
HspAI GCGC 3 cut(s) 192, 882, 929
KasI GGCGCC 1 cut(s) 881
Kpn2I TCCGGA 1 cut(s) 853
Ksp22I TGATCA 3 cut(s) 781, 892, 948
Kzo9I GATC 6 cut(s) 116, 457, 781, 787, 892, 948
LmnI GCTCC 2 cut(s) 222, 881
Lsp1109I GCAGC 2 cut(s) 223, 226
LweI GCATC 2 cut(s) 453, 565
MaeII ACGT 1 cut(s) 494
MaeIII GTNAC 2 cut(s) 695, 815
MalI GATC 6 cut(s) 118, 459, 783, 789, 894, 950
MboI GATC 6 cut(s) 116, 457, 781, 787, 892, 948
MboII GAAGA 3 cut(s) 43, 324, 411
MflI RGATCY 1 cut(s) 116
MhlI GDGCHC 1 cut(s) 878
MluCI AATT 2 cut(s) 168, 844
Mly113I GGCGCC 1 cut(s) 882
MlyI GAGTC 1 cut(s) 83
MmeI TCCRAC 4 cut(s) 199, 333, 363, 570
MroI TCCGGA 1 cut(s) 853
MseI TTAA 1 cut(s) 605
MslI CAYNNNNRTG 1 cut(s) 320
MspI CCGG 3 cut(s) 441, 854, 879
MspR9I CCNGG 2 cut(s) 153, 564
MvaI CCWGG 2 cut(s) 153, 564
MwoI GCNNNNNNNGC 1 cut(s) 935
NarI GGCGCC 1 cut(s) 882
NdeII GATC 6 cut(s) 116, 457, 781, 787, 892, 948
NlaIII CATG 4 cut(s) 114, 307, 325, 549
NlaIV GGNNCC 4 cut(s) 118, 657, 877, 883
NmeAIII GCCGAG 1 cut(s) 354
NmuCI GTSAC 1 cut(s) 695
NsbI TGCGCA 1 cut(s) 193
NspI RCATGY 2 cut(s) 114, 549
PciI ACATGT 1 cut(s) 110
PfeI GAWTC 2 cut(s) 568, 857
PkrI GCNGC 2 cut(s) 213, 216
PleI GAGTC 1 cut(s) 83
PluTI GGCGCC 1 cut(s) 885
PpsI GAGTC 1 cut(s) 83
Ppu21I YACGTR 1 cut(s) 495
PscI ACATGT 1 cut(s) 110
Psp6I CCWGG 2 cut(s) 151, 562
PspGI CCWGG 2 cut(s) 151, 562
PspN4I GGNNCC 4 cut(s) 118, 657, 877, 883
PspPI GGNCC 5 cut(s) 149, 357, 377, 592, 655
PstNI CAGNNNCTG 1 cut(s) 152
PsuI RGATCY 1 cut(s) 116
RsaI GTAC 2 cut(s) 12, 692
RsaNI GTAC 2 cut(s) 11, 691
RseI CAYNNNNRTG 1 cut(s) 320
SaqAI TTAA 1 cut(s) 605
SatI GCNGC 2 cut(s) 212, 215
Sau3AI GATC 6 cut(s) 116, 457, 781, 787, 892, 948
Sau96I GGNCC 5 cut(s) 149, 357, 377, 592, 655
SchI GAGTC 1 cut(s) 83
ScrFI CCNGG 2 cut(s) 153, 564
SduI GDGCHC 1 cut(s) 878
SfaNI GCATC 2 cut(s) 453, 565
SfoI GGCGCC 1 cut(s) 883
SinI GGWCC 2 cut(s) 592, 655
SmiMI CAYNNNNRTG 1 cut(s) 320
Sse9I AATT 2 cut(s) 168, 844
SsiI CCGC 2 cut(s) 61, 664
SspDI GGCGCC 1 cut(s) 881
SspI AATATT 1 cut(s) 27
StyD4I CCNGG 2 cut(s) 151, 562
TaaI ACNGT 3 cut(s) 695, 701, 713
TaiI ACGT 1 cut(s) 497
TasI AATT 2 cut(s) 168, 844
TatI WGTACW 1 cut(s) 690
TfiI GAWTC 2 cut(s) 568, 857
Tru1I TTAA 1 cut(s) 605
Tru9I TTAA 1 cut(s) 605
TscAI CASTG 1 cut(s) 718
TseFI GTSAC 1 cut(s) 695
TseI GCWGC 2 cut(s) 211, 214
Tsp45I GTSAC 1 cut(s) 695
TspDTI ATGAA 2 cut(s) 19, 379
TspGWI ACGGA 3 cut(s) 493, 616, 668
TspRI CASTG 1 cut(s) 718
VpaK11BI GGWCC 2 cut(s) 592, 655
XceI RCATGY 2 cut(s) 114, 549
XcmI CCANNNNNNNNNTGG 1 cut(s) 909
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.