FvH4_1g23121

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0014880
Physical Location & Seq
Forward (+)
14975631 .. 14977167
1537 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g23121.t1

Sequence Viewer

Length: 687 bp
ATGCATGCAGACCCCGAGCCACCGGAGACGACCACGTGGTCCCTCAGATTGCAGAAGATTGTCCAAGATATTCGGACTATTGCTACCTTTTTCTTCTCAGTTTGCTTTAACTATGTATACAGAGAGGCAAACTTTGTGGCGGATGCATTTGCAAATGAAGGCCACCAACTCCCGAATGGTCGTGAGTGGCAAGATGGATACTCAACAGTTGTTGCTAGAGCGCTGCTATTTGATACTGTTAACTCGGGTTGTCCGAAAGATTTGTCTTTGCATGTCGATCACCGACACCGGTACCTAGACCTCAAAAAAGTTCCACGAAACATCCTTCAGATCGTTAACGCCGTCATACTCCGTCAACATCAGCATGGAGTTGTTGAAAAACGAAAGGTATCCGTGAAGAATCCGGTTCTTCTCCTCTTGTTCATTGAATTGAAACTCGAAGATCCCACCTTCCGGCCTCTTCATGAATCACCACCTTATCTTTCAATCGCCAAACTCCCGACAACACCGCCATCAAAGCCGGCACCATCACCGACTTCTTTGATAAACATCGAACACACAAGAAGAAACCAGGGTCATCGATCAGCTCTAGGCGCCCCCCTCAAGATCCACCACCACGCGTTTCCTCCGCTAGAAGGGAAGACGCTGCCATTCTCAGAGTGCTTGAATAATTTTCCGGCTAGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-RVT

Protein Analysis

229

Amino Acids

25.87

Weight (kDa)

9.17

Isoelectric Point (pI)

53.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 37
Acc65I GGTACC 1 cut(s) 291
AccB1I GGYRCC 3 cut(s) 291, 523, 593
AccI GTMKAC 1 cut(s) 117
AccII CGCG 1 cut(s) 620
AciI CCGC 3 cut(s) 140, 509, 629
AclWI GGATC 2 cut(s) 437, 601
AcuI CTGAAG 1 cut(s) 311
AcvI CACGTG 1 cut(s) 36
AcyI GRCGYC 1 cut(s) 594
AfaI GTAC 1 cut(s) 293
AfeI AGCGCT 1 cut(s) 222
AfiI CCNNNNNNNGG 2 cut(s) 453, 635
AflIII ACRYGT 1 cut(s) 618
AgeI ACCGGT 1 cut(s) 288
AgsI TTSAA 5 cut(s) 377, 428, 433, 486, 667
AjnI CCWGG 1 cut(s) 570
AluBI AGCT 2 cut(s) 587, 684
AluI AGCT 2 cut(s) 587, 684
Alw26I GTCTC 1 cut(s) 20
AlwI GGATC 2 cut(s) 437, 601
Ama87I CYCGRG 2 cut(s) 14, 244
Aor51HI AGCGCT 1 cut(s) 222
AoxI GGCC 2 cut(s) 160, 455
ApeKI GCWGC 2 cut(s) 223, 646
ArsI GACNNNNNNTTYG 2 cut(s) 248, 280
AsiGI ACCGGT 1 cut(s) 288
Asp718I GGTACC 1 cut(s) 291
AspLEI GCGC 2 cut(s) 223, 596
AspS9I GGNCC 1 cut(s) 39
AsuHPI GGTGA 3 cut(s) 272, 462, 522
AsuNHI GCTAGC 1 cut(s) 680
AvaI CYCGRG 2 cut(s) 14, 244
AvaII GGWCC 1 cut(s) 39
BaeI ACNNNNGTAYC 2 cut(s) 275, 308
BanI GGYRCC 3 cut(s) 291, 523, 593
BbrPI CACGTG 1 cut(s) 36
BbsI GAAGAC 1 cut(s) 647
BbvI GCAGC 2 cut(s) 210, 633
BccI CCATC 3 cut(s) 188, 520, 535
BceAI ACGGC 1 cut(s) 326
BciT130I CCWGG 1 cut(s) 572
BciVI GTATCC 2 cut(s) 191, 400
BcoDI GTCTC 1 cut(s) 20
BfaI CTAG 5 cut(s) 216, 296, 590, 632, 681
BfoI RGCGCY 2 cut(s) 224, 597
BfuI GTATCC 2 cut(s) 191, 400
BisI GCNGC 2 cut(s) 224, 647
BlsI GCNGC 2 cut(s) 225, 648
Bme1390I CCNGG 1 cut(s) 572
Bme18I GGWCC 1 cut(s) 39
BmeT110I CYCGRG 2 cut(s) 14, 244
BmgT120I GGNCC 1 cut(s) 39
BmiI GGNNCC 4 cut(s) 41, 293, 525, 595
BmrFI CCNGG 1 cut(s) 572
BmsI GCATC 1 cut(s) 133
BmtI GCTAGC 1 cut(s) 684
BpiI GAAGAC 1 cut(s) 647
BpuEI CTTGAG 1 cut(s) 587
Bsa29I ATCGAT 1 cut(s) 580
BsaAI YACGTR 1 cut(s) 36
BsaBI GATNNNNATC 1 cut(s) 548
BsaHI GRCGYC 1 cut(s) 594
BsaJI CCNNGG 1 cut(s) 571
BsaWI WCCGGW 3 cut(s) 22, 288, 403
Bsc4I CCNNNNNNNGG 2 cut(s) 453, 635
Bse118I RCCGGY 2 cut(s) 288, 520
Bse8I GATNNNNATC 1 cut(s) 548
BseBI CCWGG 1 cut(s) 572
BseCI ATCGAT 1 cut(s) 580
BseDI CCNNGG 1 cut(s) 571
BseGI GGATG 2 cut(s) 148, 321
BseJI GATNNNNATC 1 cut(s) 548
BseLI CCNNNNNNNGG 2 cut(s) 453, 635
BseMII CTCAG 3 cut(s) 58, 111, 669
BseRI GAGGAG 1 cut(s) 404
BseXI GCAGC 2 cut(s) 210, 633
Bsh1236I CGCG 1 cut(s) 620
BshFI GGCC 2 cut(s) 162, 457
BshNI GGYRCC 3 cut(s) 291, 523, 593
BshTI ACCGGT 1 cut(s) 288
BshVI ATCGAT 1 cut(s) 580
BsiHKCI CYCGRG 2 cut(s) 14, 244
BsiSI CCGG 6 cut(s) 23, 289, 404, 454, 521, 677
BslFI GGGAC 1 cut(s) 25
BslI CCNNNNNNNGG 2 cut(s) 453, 635
BsmAI GTCTC 1 cut(s) 20
BsmBI CGTCTC 1 cut(s) 20
BsmFI GGGAC 1 cut(s) 25
BsnI GGCC 2 cut(s) 162, 457
BsoBI CYCGRG 2 cut(s) 14, 244
Bsp143I GATC 5 cut(s) 277, 330, 442, 581, 606
BspACI CCGC 3 cut(s) 140, 509, 629
BspANI GGCC 2 cut(s) 162, 457
BspCNI CTCAG 3 cut(s) 57, 110, 668
BspDI ATCGAT 1 cut(s) 580
BspFNI CGCG 1 cut(s) 620
BspHI TCATGA 1 cut(s) 463
BspLI GGNNCC 4 cut(s) 41, 293, 525, 595
BspOI GCTAGC 1 cut(s) 684
BspPI GGATC 2 cut(s) 437, 601
BspT107I GGYRCC 3 cut(s) 291, 523, 593
BsrFI RCCGGY 2 cut(s) 288, 520
BssAI RCCGGY 2 cut(s) 288, 520
BssECI CCNNGG 1 cut(s) 571
BssMI GATC 5 cut(s) 277, 330, 442, 581, 606
BssNAI GTATAC 1 cut(s) 118
BssNI GRCGYC 1 cut(s) 594
Bst1107I GTATAC 1 cut(s) 118
Bst2UI CCWGG 1 cut(s) 572
Bst4CI ACNGT 2 cut(s) 208, 238
Bst6I CTCTTC 1 cut(s) 465
BstACI GRCGYC 1 cut(s) 594
BstBAI YACGTR 1 cut(s) 36
BstC8I GCNNGC 3 cut(s) 6, 522, 682
BstDEI CTNAG 3 cut(s) 44, 97, 655
BstF5I GGATG 2 cut(s) 148, 321
BstFNI CGCG 1 cut(s) 620
BstH2I RGCGCY 2 cut(s) 224, 597
BstHHI GCGC 2 cut(s) 223, 596
BstKTI GATC 5 cut(s) 280, 333, 445, 584, 609
BstMAI GTCTC 1 cut(s) 20
BstMBI GATC 5 cut(s) 277, 330, 442, 581, 606
BstMWI GCNNNNNNNGC 2 cut(s) 517, 593
BstNI CCWGG 1 cut(s) 572
BstNSI RCATGY 2 cut(s) 8, 275
BstSCI CCNGG 1 cut(s) 570
BstUI CGCG 1 cut(s) 620
BstV1I GCAGC 2 cut(s) 210, 633
BstV2I GAAGAC 1 cut(s) 647
BstX2I RGATCY 2 cut(s) 442, 606
BstYI RGATCY 2 cut(s) 442, 606
BstZ17I GTATAC 1 cut(s) 118
Bsu15I ATCGAT 1 cut(s) 580
BsuI GTATCC 2 cut(s) 191, 400
BsuRI GGCC 2 cut(s) 162, 457
BsuTUI ATCGAT 1 cut(s) 580
BtsCI GGATG 2 cut(s) 148, 321
Cac8I GCNNGC 3 cut(s) 6, 522, 682
CciI TCATGA 1 cut(s) 463
CfoI GCGC 2 cut(s) 223, 596
Cfr10I RCCGGY 2 cut(s) 288, 520
Cfr13I GGNCC 1 cut(s) 39
ClaI ATCGAT 1 cut(s) 580
CseI GACGC 1 cut(s) 652
Csp6I GTAC 1 cut(s) 292
CspAI ACCGGT 1 cut(s) 288
CspCI CAANNNNNGTGG 2 cut(s) 117, 152
CviAII CATG 4 cut(s) 5, 272, 365, 464
CviJI RGCY 7 cut(s) 19, 162, 457, 520, 587, 680, 684
CviKI_1 RGCY 7 cut(s) 19, 162, 457, 520, 587, 680, 684
CviQI GTAC 1 cut(s) 292
DdeI CTNAG 3 cut(s) 44, 97, 655
DinI GGCGCC 1 cut(s) 595
DpnI GATC 5 cut(s) 279, 332, 444, 583, 608
DpnII GATC 5 cut(s) 277, 330, 442, 581, 606
DrdI GACNNNNNNGTC 1 cut(s) 37
DseDI GACNNNNNNGTC 1 cut(s) 37
Eam1104I CTCTTC 1 cut(s) 465
EarI CTCTTC 1 cut(s) 465
EciI GGCGGA 1 cut(s) 155
Eco47I GGWCC 1 cut(s) 39
Eco47III AGCGCT 1 cut(s) 222
Eco57I CTGAAG 1 cut(s) 311
Eco72I CACGTG 1 cut(s) 36
Eco88I CYCGRG 2 cut(s) 14, 244
EcoRII CCWGG 1 cut(s) 570
EcoT22I ATGCAT 2 cut(s) 6, 148
EgeI GGCGCC 1 cut(s) 595
EheI GGCGCC 1 cut(s) 595
Esp3I CGTCTC 1 cut(s) 20
FaeI CATG 4 cut(s) 8, 275, 368, 467
FaiI YATR 7 cut(s) 6, 114, 118, 273, 347, 366, 465
FaqI GGGAC 1 cut(s) 25
FatI CATG 4 cut(s) 4, 271, 364, 463
FblI GTMKAC 1 cut(s) 117
Fnu4HI GCNGC 2 cut(s) 224, 647
FokI GGATG 2 cut(s) 155, 308
Fsp4HI GCNGC 2 cut(s) 224, 647
FspBI CTAG 5 cut(s) 216, 296, 590, 632, 681
GlaI GCGC 2 cut(s) 222, 595
GluI GCNGC 2 cut(s) 224, 647
HaeII RGCGCY 2 cut(s) 224, 597
HaeIII GGCC 2 cut(s) 162, 457
HapII CCGG 6 cut(s) 23, 289, 404, 454, 521, 677
HgaI GACGC 1 cut(s) 652
HhaI GCGC 2 cut(s) 223, 596
Hin1I GRCGYC 1 cut(s) 594
Hin1II CATG 4 cut(s) 8, 275, 368, 467
Hin6I GCGC 2 cut(s) 221, 594
HinP1I GCGC 2 cut(s) 221, 594
HincII GTYRAC 3 cut(s) 241, 337, 356
HindII GTYRAC 3 cut(s) 241, 337, 356
HinfI GANTC 2 cut(s) 400, 467
HpaI GTTAAC 2 cut(s) 241, 337
HpaII CCGG 6 cut(s) 23, 289, 404, 454, 521, 677
HphI GGTGA 3 cut(s) 272, 462, 522
Hpy166II GTNNAC 4 cut(s) 118, 241, 337, 356
Hpy188I TCNGA 5 cut(s) 47, 75, 255, 330, 658
Hpy188III TCNNGA 5 cut(s) 172, 182, 464, 499, 604
Hpy8I GTNNAC 4 cut(s) 118, 241, 337, 356
HpyAV CCTTC 4 cut(s) 152, 335, 460, 629
HpyCH4III ACNGT 2 cut(s) 208, 238
HpyCH4IV ACGT 1 cut(s) 35
HpyCH4V TGCA 6 cut(s) 4, 8, 52, 146, 152, 271
HpyF10VI GCNNNNNNNGC 2 cut(s) 517, 593
HpyF3I CTNAG 3 cut(s) 44, 97, 655
HpySE526I ACGT 1 cut(s) 35
Hsp92I GRCGYC 1 cut(s) 594
Hsp92II CATG 4 cut(s) 8, 275, 368, 467
HspAI GCGC 2 cut(s) 221, 594
KasI GGCGCC 1 cut(s) 593
KpnI GGTACC 1 cut(s) 295
KroI GCCGGC 1 cut(s) 520
KroNI GCCGGC 1 cut(s) 522
KspAI GTTAAC 2 cut(s) 241, 337
Kzo9I GATC 5 cut(s) 277, 330, 442, 581, 606
LpnPI CCDG 7 cut(s) 36, 302, 417, 467, 534, 557, 584
Lsp1109I GCAGC 2 cut(s) 210, 633
LweI GCATC 1 cut(s) 133
MaeI CTAG 5 cut(s) 216, 296, 590, 632, 681
MaeII ACGT 1 cut(s) 35
MalI GATC 5 cut(s) 279, 332, 444, 583, 608
MboI GATC 5 cut(s) 277, 330, 442, 581, 606
MboII GAAGA 8 cut(s) 67, 85, 401, 409, 452, 452, 576, 652
MflI RGATCY 2 cut(s) 442, 606
MluCI AATT 2 cut(s) 428, 670
MluI ACGCGT 1 cut(s) 618
Mly113I GGCGCC 1 cut(s) 594
MnlI CCTC 7 cut(s) 53, 118, 311, 425, 468, 611, 636
Mph1103I ATGCAT 2 cut(s) 6, 148
MroNI GCCGGC 1 cut(s) 520
MseI TTAA 3 cut(s) 108, 240, 336
MslI CAYNNNNRTG 1 cut(s) 363
MspI CCGG 6 cut(s) 23, 289, 404, 454, 521, 677
MspR9I CCNGG 1 cut(s) 572
MvaI CCWGG 1 cut(s) 572
MvnI CGCG 1 cut(s) 620
MwoI GCNNNNNNNGC 2 cut(s) 517, 593
NaeI GCCGGC 1 cut(s) 522
NarI GGCGCC 1 cut(s) 594
NdeII GATC 5 cut(s) 277, 330, 442, 581, 606
NgoMIV GCCGGC 1 cut(s) 520
NheI GCTAGC 1 cut(s) 680
NlaIII CATG 4 cut(s) 8, 275, 368, 467
NlaIV GGNNCC 4 cut(s) 41, 293, 525, 595
NsiI ATGCAT 2 cut(s) 6, 148
NspI RCATGY 2 cut(s) 8, 275
PaeI GCATGC 1 cut(s) 8
PagI TCATGA 1 cut(s) 463
PcsI WCGNNNNNNNCGW 2 cut(s) 251, 339
PdiI GCCGGC 1 cut(s) 522
PfeI GAWTC 2 cut(s) 400, 467
PinAI ACCGGT 1 cut(s) 288
PkrI GCNGC 2 cut(s) 225, 648
PluTI GGCGCC 1 cut(s) 597
PmaCI CACGTG 1 cut(s) 36
PmlI CACGTG 1 cut(s) 36
Ppu21I YACGTR 1 cut(s) 36
Psp6I CCWGG 1 cut(s) 570
PspCI CACGTG 1 cut(s) 36
PspGI CCWGG 1 cut(s) 570
PspN4I GGNNCC 4 cut(s) 41, 293, 525, 595
PspPI GGNCC 1 cut(s) 39
PsuI RGATCY 2 cut(s) 442, 606
RsaI GTAC 1 cut(s) 293
RsaNI GTAC 1 cut(s) 292
RseI CAYNNNNRTG 1 cut(s) 363
SaqAI TTAA 3 cut(s) 108, 240, 336
SatI GCNGC 2 cut(s) 224, 647
Sau3AI GATC 5 cut(s) 277, 330, 442, 581, 606
Sau96I GGNCC 1 cut(s) 39
ScrFI CCNGG 1 cut(s) 572
SetI ASST 9 cut(s) 38, 89, 297, 303, 390, 452, 478, 589, 686
SfaNI GCATC 1 cut(s) 133
SfoI GGCGCC 1 cut(s) 595
SinI GGWCC 1 cut(s) 39
SmiMI CAYNNNNRTG 1 cut(s) 363
SmlI CTYRAG 1 cut(s) 602
SmoI CTYRAG 1 cut(s) 602
SphI GCATGC 1 cut(s) 8
Sse9I AATT 2 cut(s) 428, 670
SsiI CCGC 3 cut(s) 140, 509, 629
SspDI GGCGCC 1 cut(s) 593
SspMI CTAG 5 cut(s) 216, 296, 590, 632, 681
StyD4I CCNGG 1 cut(s) 570
TaaI ACNGT 2 cut(s) 208, 238
TaiI ACGT 1 cut(s) 38
TaqI TCGA 4 cut(s) 276, 438, 552, 580
TasI AATT 2 cut(s) 428, 670
TfiI GAWTC 2 cut(s) 400, 467
Tru1I TTAA 3 cut(s) 108, 240, 336
Tru9I TTAA 3 cut(s) 108, 240, 336
TseI GCWGC 2 cut(s) 223, 646
TspDTI ATGAA 4 cut(s) 171, 412, 452, 480
TspGWI ACGGA 2 cut(s) 341, 382
VpaK11BI GGWCC 1 cut(s) 39
XceI RCATGY 2 cut(s) 8, 275
XcmI CCANNNNNNNNNTGG 1 cut(s) 173
XmiI GTMKAC 1 cut(s) 117
XspI CTAG 5 cut(s) 216, 296, 590, 632, 681
Zsp2I ATGCAT 2 cut(s) 6, 148
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.