Rmu_sc0001767.1_g000001

ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001767.1
OG0014880
Physical Location & Seq
Forward (+)
2200 .. 3293
1094 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001767.1_g000001.1.cds

Sequence Viewer

Length: 561 bp
atgcatcatgtgcttgacgtcgtcgtcgttggtgaccgagatcaaggcgtcaaggtcttcctcggggagctggtacttgaacgacacgtcgttgttagagagggcggagagcctgccgatgagggcggagaacttgatggtgcgttcaacggtgaggatcttggtctcgccgtcggtgtaggtaaaagagaaacagaagaattcgatggcctggaagggtatcctggatgcaagaacctcattttgaagggcatgcgctggatcgttggtaatggtaagactataaagttttggacttttaattgggtctttgatacgccccttttaaattttattcctgaaaatcgcaagcatttgatcaacttagaagagactgtggacgattatgtgttacatggctcctggaataaaccaaagcttctagctttcctggactcgtcaattgtaaaagaaatccttggtattcctttgccagtatatgatcaagaagacaattttatctggggcttctgtgaatggacgcttcataatcaaatcggctacatggcttcaattacatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1 RVT_3 zf-RVT

Protein Analysis

186

Amino Acids

20.97

Weight (kDa)

4.72

Isoelectric Point (pI)

25.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 23
AatII GACGTC 1 cut(s) 21
AciI CCGC 2 cut(s) 105, 126
AclWI GGATC 2 cut(s) 165, 269
AcsI RAATTY 2 cut(s) 200, 328
AcyI GRCGYC 2 cut(s) 18, 48
AfaI GTAC 1 cut(s) 75
AflIII ACRYGT 1 cut(s) 85
AgsI TTSAA 4 cut(s) 80, 148, 247, 552
AjiI CACGTC 1 cut(s) 88
AjnI CCWGG 4 cut(s) 210, 223, 401, 429
AluBI AGCT 3 cut(s) 70, 418, 425
AluI AGCT 3 cut(s) 70, 418, 425
Alw26I GTCTC 2 cut(s) 170, 365
AlwI GGATC 2 cut(s) 165, 269
Ama87I CYCGRG 1 cut(s) 62
AoxI GGCC 1 cut(s) 208
ApoI RAATTY 2 cut(s) 200, 328
AspLEI GCGC 1 cut(s) 258
AsuHPI GGTGA 2 cut(s) 44, 164
AvaI CYCGRG 1 cut(s) 62
BbsI GAAGAC 2 cut(s) 49, 495
BccI CCATC 2 cut(s) 131, 200
BceAI ACGGC 1 cut(s) 155
BciT130I CCWGG 4 cut(s) 212, 225, 403, 431
BciVI GTATCC 1 cut(s) 231
BclI TGATCA 2 cut(s) 357, 481
BcoDI GTCTC 2 cut(s) 170, 365
BfaI CTAG 1 cut(s) 422
BfuI GTATCC 1 cut(s) 231
Bme1390I CCNGG 4 cut(s) 212, 225, 403, 431
BmeT110I CYCGRG 1 cut(s) 62
BmgBI CACGTC 1 cut(s) 88
BmiI GGNNCC 1 cut(s) 400
BmrFI CCNGG 4 cut(s) 212, 225, 403, 431
BmsI GCATC 2 cut(s) 13, 218
BpiI GAAGAC 2 cut(s) 49, 495
BsaHI GRCGYC 2 cut(s) 18, 48
BsaI GGTCTC 1 cut(s) 170
BsaJI CCNNGG 2 cut(s) 61, 457
Bse1I ACTGG 1 cut(s) 473
BseBI CCWGG 4 cut(s) 212, 225, 403, 431
BseDI CCNNGG 2 cut(s) 61, 457
BseGI GGATG 1 cut(s) 233
BseNI ACTGG 1 cut(s) 473
BshFI GGCC 1 cut(s) 210
BsiHKCI CYCGRG 1 cut(s) 62
BsmAI GTCTC 2 cut(s) 170, 365
BsnI GGCC 1 cut(s) 210
Bso31I GGTCTC 1 cut(s) 170
BsoBI CYCGRG 1 cut(s) 62
Bsp143I GATC 5 cut(s) 40, 157, 261, 357, 481
BspACI CCGC 2 cut(s) 105, 126
BspANI GGCC 1 cut(s) 210
BspLI GGNNCC 1 cut(s) 400
BspPI GGATC 2 cut(s) 165, 269
BspTNI GGTCTC 1 cut(s) 170
BsrI ACTGG 1 cut(s) 473
BssECI CCNNGG 2 cut(s) 61, 457
BssMI GATC 5 cut(s) 40, 157, 261, 357, 481
BssNI GRCGYC 2 cut(s) 18, 48
BssT1I CCWWGG 1 cut(s) 457
Bst2UI CCWGG 4 cut(s) 212, 225, 403, 431
Bst4CI ACNGT 2 cut(s) 152, 376
Bst6I CTCTTC 1 cut(s) 363
BstACI GRCGYC 2 cut(s) 18, 48
BstAPI GCANNNNNTGC 1 cut(s) 10
BstC8I GCNNGC 3 cut(s) 114, 254, 350
BstDEI CTNAG 1 cut(s) 364
BstEII GGTNACC 1 cut(s) 32
BstF5I GGATG 1 cut(s) 233
BstHHI GCGC 1 cut(s) 258
BstKTI GATC 5 cut(s) 43, 160, 264, 360, 484
BstMAI GTCTC 2 cut(s) 170, 365
BstMBI GATC 5 cut(s) 40, 157, 261, 357, 481
BstMWI GCNNNNNNNGC 1 cut(s) 10
BstNI CCWGG 4 cut(s) 212, 225, 403, 431
BstNSI RCATGY 1 cut(s) 256
BstPI GGTNACC 1 cut(s) 32
BstSCI CCNGG 4 cut(s) 210, 223, 401, 429
BstV2I GAAGAC 2 cut(s) 49, 495
BstX2I RGATCY 1 cut(s) 157
BstYI RGATCY 1 cut(s) 157
BsuI GTATCC 1 cut(s) 231
BsuRI GGCC 1 cut(s) 210
BtrI CACGTC 1 cut(s) 88
BtsCI GGATG 1 cut(s) 233
Cac8I GCNNGC 3 cut(s) 114, 254, 350
CfoI GCGC 1 cut(s) 258
CseI GACGC 2 cut(s) 37, 529
Csp6I GTAC 1 cut(s) 74
CviAII CATG 5 cut(s) 8, 253, 395, 544, 558
CviJI RGCY 9 cut(s) 70, 112, 210, 399, 418, 425, 507, 540, 548
CviKI_1 RGCY 9 cut(s) 70, 112, 210, 399, 418, 425, 507, 540, 548
CviQI GTAC 1 cut(s) 74
DdeI CTNAG 1 cut(s) 364
DpnI GATC 5 cut(s) 42, 159, 263, 359, 483
DpnII GATC 5 cut(s) 40, 157, 261, 357, 481
DraI TTTAAA 1 cut(s) 327
DrdI GACNNNNNNGTC 1 cut(s) 23
DseDI GACNNNNNNGTC 1 cut(s) 23
Eam1104I CTCTTC 1 cut(s) 363
EarI CTCTTC 1 cut(s) 363
EciI GGCGGA 2 cut(s) 120, 141
Eco130I CCWWGG 1 cut(s) 457
Eco31I GGTCTC 1 cut(s) 170
Eco88I CYCGRG 1 cut(s) 62
Eco91I GGTNACC 1 cut(s) 32
EcoO65I GGTNACC 1 cut(s) 32
EcoRI GAATTC 1 cut(s) 200
EcoRII CCWGG 4 cut(s) 210, 223, 401, 429
EcoT14I CCWWGG 1 cut(s) 457
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 457
FaeI CATG 5 cut(s) 11, 256, 398, 547, 561
FalI AAGNNNNNCTT 2 cut(s) 441, 473
FatI CATG 5 cut(s) 7, 252, 394, 543, 557
FbaI TGATCA 2 cut(s) 357, 481
FokI GGATG 1 cut(s) 240
FspBI CTAG 1 cut(s) 422
GlaI GCGC 1 cut(s) 257
HaeIII GGCC 1 cut(s) 210
HgaI GACGC 2 cut(s) 37, 529
HhaI GCGC 1 cut(s) 258
Hin1I GRCGYC 2 cut(s) 18, 48
Hin1II CATG 5 cut(s) 11, 256, 398, 547, 561
Hin6I GCGC 1 cut(s) 256
HinP1I GCGC 1 cut(s) 256
HindIII AAGCTT 1 cut(s) 416
HinfI GANTC 1 cut(s) 434
HphI GGTGA 2 cut(s) 44, 164
Hpy166II GTNNAC 1 cut(s) 379
Hpy188III TCNNGA 2 cut(s) 338, 485
Hpy8I GTNNAC 1 cut(s) 379
Hpy99I CGWCG 5 cut(s) 23, 26, 29, 92, 176
HpyAV CCTTC 2 cut(s) 209, 241
HpyCH4III ACNGT 2 cut(s) 152, 376
HpyCH4IV ACGT 2 cut(s) 18, 87
HpyCH4V TGCA 2 cut(s) 4, 231
HpyF10VI GCNNNNNNNGC 1 cut(s) 10
HpyF3I CTNAG 1 cut(s) 364
HpySE526I ACGT 2 cut(s) 18, 87
Hsp92I GRCGYC 2 cut(s) 18, 48
Hsp92II CATG 5 cut(s) 11, 256, 398, 547, 561
HspAI GCGC 1 cut(s) 256
Ksp22I TGATCA 2 cut(s) 357, 481
Kzo9I GATC 5 cut(s) 40, 157, 261, 357, 481
LmnI GCTCC 2 cut(s) 67, 404
LweI GCATC 2 cut(s) 13, 218
MaeI CTAG 1 cut(s) 422
MaeII ACGT 2 cut(s) 18, 87
MaeIII GTNAC 2 cut(s) 32, 390
MalI GATC 5 cut(s) 42, 159, 263, 359, 483
MboI GATC 5 cut(s) 40, 157, 261, 357, 481
MboII GAAGA 4 cut(s) 49, 209, 380, 500
MfeI CAATTG 1 cut(s) 441
MflI RGATCY 1 cut(s) 157
MluCI AATT 6 cut(s) 200, 301, 328, 441, 493, 552
MlyI GAGTC 1 cut(s) 428
MnlI CCTC 5 cut(s) 71, 94, 115, 148, 248
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 2 cut(s) 300, 326
MspR9I CCNGG 4 cut(s) 212, 225, 403, 431
MunI CAATTG 1 cut(s) 441
MvaI CCWGG 4 cut(s) 212, 225, 403, 431
MwoI GCNNNNNNNGC 1 cut(s) 10
NdeII GATC 5 cut(s) 40, 157, 261, 357, 481
NlaIII CATG 5 cut(s) 11, 256, 398, 547, 561
NlaIV GGNNCC 1 cut(s) 400
NmuCI GTSAC 1 cut(s) 32
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 256
PaeI GCATGC 1 cut(s) 256
PcsI WCGNNNNNNNCGW 1 cut(s) 24
PflFI GACNNNGTC 1 cut(s) 20
PfoI TCCNGGA 3 cut(s) 223, 401, 429
PleI GAGTC 1 cut(s) 428
PpsI GAGTC 1 cut(s) 428
Psp6I CCWGG 4 cut(s) 210, 223, 401, 429
PspEI GGTNACC 1 cut(s) 32
PspGI CCWGG 4 cut(s) 210, 223, 401, 429
PspN4I GGNNCC 1 cut(s) 400
PsuI RGATCY 1 cut(s) 157
PsyI GACNNNGTC 1 cut(s) 20
RsaI GTAC 1 cut(s) 75
RsaNI GTAC 1 cut(s) 74
SaqAI TTAA 2 cut(s) 300, 326
Sau3AI GATC 5 cut(s) 40, 157, 261, 357, 481
SchI GAGTC 1 cut(s) 428
ScrFI CCNGG 4 cut(s) 212, 225, 403, 431
SetI ASST 8 cut(s) 21, 57, 72, 90, 184, 240, 420, 427
SfaNI GCATC 2 cut(s) 13, 218
SphI GCATGC 1 cut(s) 256
Sse9I AATT 6 cut(s) 200, 301, 328, 441, 493, 552
SsiI CCGC 2 cut(s) 105, 126
SspMI CTAG 1 cut(s) 422
StyD4I CCNGG 4 cut(s) 210, 223, 401, 429
StyI CCWWGG 1 cut(s) 457
TaaI ACNGT 2 cut(s) 152, 376
TaiI ACGT 2 cut(s) 21, 90
TaqI TCGA 1 cut(s) 204
TaqII GACCGA 1 cut(s) 51
TasI AATT 6 cut(s) 200, 301, 328, 441, 493, 552
Tru1I TTAA 2 cut(s) 300, 326
Tru9I TTAA 2 cut(s) 300, 326
TseFI GTSAC 1 cut(s) 32
Tsp45I GTSAC 1 cut(s) 32
TspDTI ATGAA 1 cut(s) 515
Tth111I GACNNNGTC 1 cut(s) 20
XapI RAATTY 2 cut(s) 200, 328
XceI RCATGY 1 cut(s) 256
XspI CTAG 1 cut(s) 422
ZraI GACGTC 1 cut(s) 19
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.