FvH4_1g25250

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0015319
Physical Location & Seq
Forward (+)
17046250 .. 17048361
2112 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g25250.t2

Sequence Viewer

Length: 780 bp
ATGCTGGCAGTAAAGCTGGTCCGTTCTCTGGTGTTGGGAGAAACCATCAACAACAACCCTGTCGTTGACGACTCCTCCGACTCCGACCAAAACGACGTCGTGGTGGCCACCAAACCCAAAACCCCACTGCTCCTCTTCCTCCCAACCAAAGAGCTCGTCACCGACACCTACAGACTCGCCACCATCGCCCGCGACATGGGCTTCGACCTCCACCCAACCCCTTCTCTCTCCCACATCCTCCTCTCCCACCCTCCGTCTTCTTCGCCTTCTTCCTCGCCGTCGACCTCGTTTCCTTCCACGTGGTCATCCACATCTCTCTCTTCCTCTTCCTCTCTCCCGGAGGCCACCGTCCCCCTCCCTTTCCCTTCCCTCATCGGAGCTCCGGTGCCGCATCTCCGCGCCTTCGTCAAGCTCTCCCGGGGCCTCTTCAAGCTCGTCTTCGCGCCGGCGGAGCTCATAGACAGTGAATCGGCGAGTGTTGAGAGCTACTGGGACTGCTGCTCCGGGTCGCTGTTTTCGCGGCTCGCCGGAGATAGGATTGGCACTATGGAGGGGTTTTCGAGGGCCCTGGCCGGAGCTGGGTGGAGTCTTTTCAAGACTAGGGAGAATGGCGGTGGGAGATCGGTGTATTTGTTCAGGAAGGTTGATGTAAACAGGGTTCGGGATGGGGAGTGTAGGATAAGGGAGCTGAGGTTGCCTGCATTGGATTTTCGGAATGCTCCTTTGCGGATTTTGCAGTACATTCTTCTTATGACTGATGATATGTTCTATCTCGCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

260

Amino Acids

28.32

Weight (kDa)

6.91

Isoelectric Point (pI)

50.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015319)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G08770
fragaria_vesca FvH4_1g25250 FvH4_1g25250
malus_domestica MD15G1137000.v1.1 MD15G1137300.v1.1
prunus_persica Prupe.4G279900_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0123171
rosa_multiflora Rmu_co8220114.1_g000001
rosa_roxburghii Rroxscaffold_2G00120860
rosa_rugosa Rorug02G0243600
rosa_samantha Rh2AG303100 Rh2BG311000 Rh2CG289400 Rh2DG327100
rosa_wichuraiana Rw2G024140 Rw2G024230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 99
AccB1I GGYRCC 1 cut(s) 385
AccI GTMKAC 1 cut(s) 281
AccII CGCG 4 cut(s) 192, 399, 443, 520
AciI CCGC 7 cut(s) 190, 389, 397, 449, 520, 612, 727
AcoI YGGCCR 2 cut(s) 105, 570
AcvI CACGTG 1 cut(s) 300
AcyI GRCGYC 1 cut(s) 96
AfaI GTAC 1 cut(s) 740
AfiI CCNNNNNNNGG 4 cut(s) 28, 196, 534, 579
AgsI TTSAA 2 cut(s) 430, 595
AjnI CCWGG 1 cut(s) 567
AluBI AGCT 9 cut(s) 16, 154, 380, 412, 433, 454, 486, 578, 688
AluI AGCT 9 cut(s) 16, 154, 380, 412, 433, 454, 486, 578, 688
Alw21I GWGCWC 3 cut(s) 156, 382, 456
Ama87I CYCGRG 1 cut(s) 417
AoxI GGCC 5 cut(s) 105, 342, 421, 564, 570
ApaI GGGCCC 1 cut(s) 568
ApeKI GCWGC 1 cut(s) 498
ArsI GACNNNNNNTTYG 4 cut(s) 141, 173, 185, 217
AspLEI GCGC 2 cut(s) 401, 445
AspS9I GGNCC 4 cut(s) 19, 421, 564, 565
AsuC2I CCSGG 4 cut(s) 338, 418, 419, 505
AsuHPI GGTGA 1 cut(s) 151
AvaI CYCGRG 1 cut(s) 417
AvaII GGWCC 1 cut(s) 19
BaeGI GKGCMC 1 cut(s) 568
BalI TGGCCA 1 cut(s) 107
BanI GGYRCC 1 cut(s) 385
BanII GRGCYC 4 cut(s) 156, 382, 456, 568
BbrPI CACGTG 1 cut(s) 300
BbsI GAAGAC 2 cut(s) 249, 430
Bbv12I GWGCWC 3 cut(s) 156, 382, 456
BbvCI CCTCAGC 1 cut(s) 689
BbvI GCAGC 1 cut(s) 485
BccI CCATC 3 cut(s) 53, 191, 659
BceAI ACGGC 1 cut(s) 262
BciT130I CCWGG 1 cut(s) 569
BcnI CCSGG 4 cut(s) 338, 418, 419, 505
BfaI CTAG 1 cut(s) 600
BfmI CTRYAG 1 cut(s) 169
BisI GCNGC 3 cut(s) 389, 499, 521
BlsI GCNGC 3 cut(s) 390, 500, 522
Bme1390I CCNGG 5 cut(s) 338, 418, 419, 505, 569
Bme18I GGWCC 1 cut(s) 19
BmeT110I CYCGRG 1 cut(s) 417
BmgT120I GGNCC 4 cut(s) 19, 421, 564, 565
BmiI GGNNCC 3 cut(s) 387, 422, 566
BmrFI CCNGG 5 cut(s) 338, 418, 419, 505, 569
BmrI ACTGGG 1 cut(s) 499
BmsI GCATC 1 cut(s) 400
BmuI ACTGGG 1 cut(s) 499
BpiI GAAGAC 2 cut(s) 249, 430
Bpu10I CCTNAGC 1 cut(s) 689
BpuMI CCSGG 4 cut(s) 338, 418, 419, 505
BsaAI YACGTR 1 cut(s) 300
BsaHI GRCGYC 1 cut(s) 96
BsaJI CCNNGG 3 cut(s) 417, 418, 567
BsaWI WCCGGW 1 cut(s) 382
BsaXI ACNNNNNCTCC 8 cut(s) 59, 89, 369, 399, 485, 515, 677, 707
Bsc4I CCNNNNNNNGG 4 cut(s) 28, 196, 534, 579
Bse118I RCCGGY 1 cut(s) 445
Bse1I ACTGG 1 cut(s) 494
BseBI CCWGG 1 cut(s) 569
BseDI CCNNGG 3 cut(s) 417, 418, 567
BseGI GGATG 3 cut(s) 234, 305, 670
BseLI CCNNNNNNNGG 4 cut(s) 28, 196, 534, 579
BseMII CTCAG 1 cut(s) 680
BseNI ACTGG 1 cut(s) 494
BseRI GAGGAG 3 cut(s) 64, 122, 230
BseSI GKGCMC 1 cut(s) 568
BseXI GCAGC 1 cut(s) 485
BseYI CCCAGC 1 cut(s) 578
Bsh1236I CGCG 4 cut(s) 192, 399, 443, 520
BshFI GGCC 5 cut(s) 107, 344, 423, 566, 572
BshNI GGYRCC 1 cut(s) 385
BsiHKAI GWGCWC 3 cut(s) 156, 382, 456
BsiHKCI CYCGRG 1 cut(s) 417
BsiSI CCGG 7 cut(s) 338, 383, 418, 446, 504, 528, 573
BslFI GGGAC 2 cut(s) 335, 506
BslI CCNNNNNNNGG 4 cut(s) 28, 196, 534, 579
BsmFI GGGAC 2 cut(s) 335, 506
BsmI GAATGC 1 cut(s) 721
BsnI GGCC 5 cut(s) 107, 344, 423, 566, 572
BsoBI CYCGRG 1 cut(s) 417
Bsp120I GGGCCC 1 cut(s) 564
Bsp1286I GDGCHC 4 cut(s) 156, 382, 456, 568
Bsp143I GATC 1 cut(s) 620
BspACI CCGC 7 cut(s) 190, 389, 397, 449, 520, 612, 727
BspANI GGCC 5 cut(s) 107, 344, 423, 566, 572
BspCNI CTCAG 1 cut(s) 681
BspFNI CGCG 4 cut(s) 192, 399, 443, 520
BspLI GGNNCC 3 cut(s) 387, 422, 566
BspT107I GGYRCC 1 cut(s) 385
BsrFI RCCGGY 1 cut(s) 445
BsrI ACTGG 1 cut(s) 494
BssAI RCCGGY 1 cut(s) 445
BssECI CCNNGG 3 cut(s) 417, 418, 567
BssMI GATC 1 cut(s) 620
BssNI GRCGYC 1 cut(s) 96
Bst2UI CCWGG 1 cut(s) 569
Bst4CI ACNGT 2 cut(s) 349, 464
Bst6I CTCTTC 4 cut(s) 140, 325, 331, 431
BstACI GRCGYC 1 cut(s) 96
BstBAI YACGTR 1 cut(s) 300
BstC8I GCNNGC 5 cut(s) 6, 190, 447, 525, 699
BstDEI CTNAG 1 cut(s) 689
BstF5I GGATG 3 cut(s) 234, 305, 670
BstFNI CGCG 4 cut(s) 192, 399, 443, 520
BstHHI GCGC 2 cut(s) 401, 445
BstKTI GATC 1 cut(s) 623
BstMBI GATC 1 cut(s) 620
BstMWI GCNNNNNNNGC 6 cut(s) 185, 198, 451, 517, 694, 733
BstNI CCWGG 1 cut(s) 569
BstSCI CCNGG 5 cut(s) 336, 416, 417, 503, 567
BstSFI CTRYAG 1 cut(s) 169
BstSLI GKGCMC 1 cut(s) 568
BstUI CGCG 4 cut(s) 192, 399, 443, 520
BstV1I GCAGC 1 cut(s) 485
BstV2I GAAGAC 2 cut(s) 249, 430
BsuRI GGCC 5 cut(s) 107, 344, 423, 566, 572
BtgZI GCGATG 1 cut(s) 169
BtsCI GGATG 3 cut(s) 234, 305, 670
BtsI GCAGTG 1 cut(s) 125
BtsIMutI CAGTG 2 cut(s) 125, 469
Cac8I GCNNGC 5 cut(s) 6, 190, 447, 525, 699
CfoI GCGC 2 cut(s) 401, 445
Cfr10I RCCGGY 1 cut(s) 445
Cfr13I GGNCC 4 cut(s) 19, 421, 564, 565
Cfr9I CCCGGG 1 cut(s) 417
Csp6I GTAC 1 cut(s) 739
CviAII CATG 2 cut(s) 196, 777
CviQI GTAC 1 cut(s) 739
DdeI CTNAG 1 cut(s) 689
DpnI GATC 1 cut(s) 622
DpnII GATC 1 cut(s) 620
EaeI YGGCCR 2 cut(s) 105, 570
Eam1104I CTCTTC 4 cut(s) 140, 325, 331, 431
EarI CTCTTC 4 cut(s) 140, 325, 331, 431
EciI GGCGGA 1 cut(s) 464
Ecl136II GAGCTC 3 cut(s) 154, 380, 454
Eco24I GRGCYC 4 cut(s) 156, 382, 456, 568
Eco47I GGWCC 1 cut(s) 19
Eco53kI GAGCTC 3 cut(s) 154, 380, 454
Eco72I CACGTG 1 cut(s) 300
Eco88I CYCGRG 1 cut(s) 417
EcoICRI GAGCTC 3 cut(s) 154, 380, 454
EcoO109I RGGNCCY 3 cut(s) 421, 564, 565
EcoRII CCWGG 1 cut(s) 567
EcoT38I GRGCYC 4 cut(s) 156, 382, 456, 568
FaeI CATG 2 cut(s) 199, 780
FaiI YATR 6 cut(s) 197, 458, 548, 752, 764, 778
FalI AAGNNNNNCTT 2 cut(s) 422, 454
FaqI GGGAC 2 cut(s) 335, 506
FatI CATG 2 cut(s) 195, 776
FauI CCCGC 1 cut(s) 197
FblI GTMKAC 1 cut(s) 281
Fnu4HI GCNGC 3 cut(s) 389, 499, 521
FokI GGATG 3 cut(s) 221, 292, 677
FriOI GRGCYC 4 cut(s) 156, 382, 456, 568
Fsp4HI GCNGC 3 cut(s) 389, 499, 521
FspBI CTAG 1 cut(s) 600
GlaI GCGC 2 cut(s) 400, 444
GluI GCNGC 3 cut(s) 389, 499, 521
GsaI CCCAGC 1 cut(s) 582
HaeIII GGCC 5 cut(s) 107, 344, 423, 566, 572
HapII CCGG 7 cut(s) 338, 383, 418, 446, 504, 528, 573
HhaI GCGC 2 cut(s) 401, 445
Hin1I GRCGYC 1 cut(s) 96
Hin1II CATG 2 cut(s) 199, 780
Hin6I GCGC 2 cut(s) 399, 443
HinP1I GCGC 2 cut(s) 399, 443
HincII GTYRAC 2 cut(s) 67, 282
HindII GTYRAC 2 cut(s) 67, 282
HinfI GANTC 5 cut(s) 71, 80, 174, 467, 586
HpaII CCGG 7 cut(s) 338, 383, 418, 446, 504, 528, 573
HphI GGTGA 1 cut(s) 151
Hpy166II GTNNAC 3 cut(s) 67, 282, 652
Hpy188I TCNGA 4 cut(s) 79, 85, 377, 714
Hpy188III TCNNGA 3 cut(s) 595, 637, 662
Hpy8I GTNNAC 3 cut(s) 67, 282, 652
Hpy99I CGWCG 3 cut(s) 98, 101, 283
HpyAV CCTTC 6 cut(s) 231, 276, 303, 375, 412, 634
HpyCH4III ACNGT 2 cut(s) 349, 464
HpyCH4IV ACGT 2 cut(s) 96, 299
HpyCH4V TGCA 2 cut(s) 701, 736
HpyF10VI GCNNNNNNNGC 6 cut(s) 185, 198, 451, 517, 694, 733
HpyF3I CTNAG 1 cut(s) 689
HpySE526I ACGT 2 cut(s) 96, 299
Hsp92I GRCGYC 1 cut(s) 96
Hsp92II CATG 2 cut(s) 199, 780
HspAI GCGC 2 cut(s) 399, 443
KroI GCCGGC 1 cut(s) 445
KroNI GCCGGC 1 cut(s) 447
Kzo9I GATC 1 cut(s) 620
LmnI GCTCC 8 cut(s) 135, 377, 385, 451, 506, 575, 685, 724
Lsp1109I GCAGC 1 cut(s) 485
LweI GCATC 1 cut(s) 400
MaeI CTAG 1 cut(s) 600
MaeII ACGT 2 cut(s) 96, 299
MaeIII GTNAC 1 cut(s) 157
MalI GATC 1 cut(s) 622
MboI GATC 1 cut(s) 620
MboII GAAGA 9 cut(s) 127, 249, 252, 261, 312, 318, 418, 430, 737
MhlI GDGCHC 4 cut(s) 156, 382, 456, 568
MlsI TGGCCA 1 cut(s) 107
MluNI TGGCCA 1 cut(s) 107
MlyI GAGTC 4 cut(s) 65, 74, 168, 595
MmeI TCCRAC 2 cut(s) 102, 108
Mox20I TGGCCA 1 cut(s) 107
MreI CGCCGGCG 1 cut(s) 445
MroNI GCCGGC 1 cut(s) 445
MscI TGGCCA 1 cut(s) 107
Msp20I TGGCCA 1 cut(s) 107
MspI CCGG 7 cut(s) 338, 383, 418, 446, 504, 528, 573
MspR9I CCNGG 5 cut(s) 338, 418, 419, 505, 569
Mva1269I GAATGC 1 cut(s) 721
MvaI CCWGG 1 cut(s) 569
MvnI CGCG 4 cut(s) 192, 399, 443, 520
MwoI GCNNNNNNNGC 6 cut(s) 185, 198, 451, 517, 694, 733
NaeI GCCGGC 1 cut(s) 447
NciI CCSGG 4 cut(s) 338, 418, 419, 505
NdeII GATC 1 cut(s) 620
NgoMIV GCCGGC 1 cut(s) 445
NlaIII CATG 2 cut(s) 199, 780
NlaIV GGNNCC 3 cut(s) 387, 422, 566
NmuCI GTSAC 1 cut(s) 157
PcsI WCGNNNNNNNCGW 1 cut(s) 75
PctI GAATGC 1 cut(s) 721
PdiI GCCGGC 1 cut(s) 447
PfeI GAWTC 1 cut(s) 467
PfoI TCCNGGA 1 cut(s) 336
PkrI GCNGC 3 cut(s) 390, 500, 522
PleI GAGTC 4 cut(s) 65, 74, 168, 594
PmaCI CACGTG 1 cut(s) 300
PmlI CACGTG 1 cut(s) 300
PpsI GAGTC 4 cut(s) 65, 74, 168, 594
Ppu21I YACGTR 1 cut(s) 300
Psp124BI GAGCTC 3 cut(s) 156, 382, 456
Psp6I CCWGG 1 cut(s) 567
PspCI CACGTG 1 cut(s) 300
PspFI CCCAGC 1 cut(s) 578
PspGI CCWGG 1 cut(s) 567
PspN4I GGNNCC 3 cut(s) 387, 422, 566
PspOMI GGGCCC 1 cut(s) 564
PspPI GGNCC 4 cut(s) 19, 421, 564, 565
PsrI GAACNNNNNNTAC 2 cut(s) 642, 674
RsaI GTAC 1 cut(s) 740
RsaNI GTAC 1 cut(s) 739
SacI GAGCTC 3 cut(s) 156, 382, 456
SalI GTCGAC 1 cut(s) 280
SatI GCNGC 3 cut(s) 389, 499, 521
Sau3AI GATC 1 cut(s) 620
Sau96I GGNCC 4 cut(s) 19, 421, 564, 565
SchI GAGTC 4 cut(s) 65, 74, 168, 595
ScrFI CCNGG 5 cut(s) 338, 418, 419, 505, 569
SduI GDGCHC 4 cut(s) 156, 382, 456, 568
SfaNI GCATC 1 cut(s) 400
SfcI CTRYAG 1 cut(s) 169
SgrAI CRCCGGYG 1 cut(s) 445
SinI GGWCC 1 cut(s) 19
SmaI CCCGGG 1 cut(s) 419
SsiI CCGC 7 cut(s) 190, 389, 397, 449, 520, 612, 727
SspMI CTAG 1 cut(s) 600
SstI GAGCTC 3 cut(s) 156, 382, 456
StyD4I CCNGG 5 cut(s) 336, 416, 417, 503, 567
TaaI ACNGT 2 cut(s) 349, 464
TaiI ACGT 2 cut(s) 99, 302
TaqI TCGA 3 cut(s) 204, 281, 560
TatI WGTACW 1 cut(s) 738
TauI GCSGC 2 cut(s) 391, 523
TfiI GAWTC 1 cut(s) 467
TscAI CASTG 2 cut(s) 132, 469
TseFI GTSAC 1 cut(s) 157
TseI GCWGC 1 cut(s) 498
Tsp45I GTSAC 1 cut(s) 157
TspGWI ACGGA 2 cut(s) 11, 243
TspMI CCCGGG 1 cut(s) 417
TspRI CASTG 2 cut(s) 132, 469
VpaK11BI GGWCC 1 cut(s) 19
XmaI CCCGGG 1 cut(s) 417
XmiI GTMKAC 1 cut(s) 281
XspI CTAG 1 cut(s) 600
ZraI GACGTC 1 cut(s) 97
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.