Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0010361

Physical Location & Seq

Genomic Coordinates Forward (+)

18820944 .. 18825794

Gene Structure 4851 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g27040.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2052 bp

ATGATTAGTACTCTTGGTAGGTTAGGGAAGGTTGAGCTTGCCAAGAATGTGTTTCAAACTGCTGTCAATGAGGGTTATGGCAGGACTGTTTATACTTATTCGGCTTTGATTAGTGCTTACGGGCGCAGCGGGTATTGTGACGAGGCTATCAGGGTGCTCGAGTCCATGAAAGATTCGGGCGTGAAGCCGAATTTGGTTACTTACAATGCGGTGATTGATGCGTGTGGGAAAGGGGGCGTGGAGTTTAAGAAGGTGGTGGAGATTTTCGATGAGATGCTGAAAGTCGGGGTGCAGCCTGATCGGATTACTTATAATTCTCTCCTTGCGGTTTGTAGCCGAGGGGGACTGTGGGAGGCGGCTAGAAACTTGTTCAGTGAGATGGTGGATAGAGGGATTGATCAGGATATTTATACTTATAATACGCTTTTGGATGCAATTTCTAAAGGTGGGCAGATGGATTTGGCATATAAGATTATGTCGGAGATGCCTTCAAAGAATATTTTGCCTAATGTGGTGACTTACAGTACTATGATTGATGGTTATGCAAAGGCTGGAAGATTAGAAGATGCCCTCAATTTATTTAATGAGATGAAGTTTTTAGCCATTGGTCTGGATAGAGTCCTGTATAATACATTGCTGTCACTTTATGGGAAGCTTGGCAGGTTTGAGGAGGCTTTGAATGTTTGCAAGGAGATGGAGAGTGTTGGGATTGCGAAGGACGTCGTATCTTATAATGCACTTCTTGGTGGGTATGGGAAGCAAGGGAAGTATGATGAAGTTAAAGGACTGTACAATGAAATGAAAGTAGAACGTGTATCACCAAATCTATTAACATATTCAACATTGATTGATGTGTATTCAAAAGGTGGTTTGTATGCGGAGGCAGTGAAGGTTTTTAGGGAGTTCAAGCAGGCAGGGCTCAAGGCTGATGTCGTGCTATATAGTGAACTTATTAATGCTTTGTGCAAAAATGGGCTGGTGGAATCTGCTGTTTCCTTGCTTGATGAGATGACCAAGGAAGGCATCCGACCTAATGTTGTCACTTATAATTCTATAATTGATGCCTTTGGTAGGCCAGCGACAACAGTGTGTGCAGTTGATGCTGGTGCCTGTGGCATTGTGCTACGCAGTGAATCTTCATCTTCAATTTCTGCAAGGGATTTTGACATTTCTGACAAGAATGTTCAAAATGAGATGAGAGATAGGGAGGATACAAGAATTATGAAGATGTTTGGGCAACTTACAGCTGATAAAGCTGGTTATGCAAAGAAAGATAGGAAGGTCAGACAAGAAATCTTGTGCATTTTGGGTGTCTTTCAGAAGATGCATGAACTCGACATCAAACCAAATGTCGTCACATTTTCAGCTATTCTAAATGCTTGCAGCCGCTGTAATTCATTTGAAGATGCTTCCATGTTATTGGAGGAACTGCGGTTGTTTGATAACCAGGTTTATGGTGTTGCCCATGGACTTCTTATGGGCTGCAGGGGTAATGTATGGGTAAAAGCTCAGTCTCTCTTCGATGAAGTTAAGCAGATGGACTGTTCAACTGCATCTGCTTTCTACAATGCTCTTACCGACATGCTATGGCACTTTGGACAGAAAAAAGGGGCGCAATTGGTTGTGCTTGAAGGGGAGCGCCGAAATGTATGGGAGAATGCATGGTCTAATTCACGATTGGATTTGCATTTGATGTCTTCTGGGGCTGCTCGGGCAATGGTTCATGCATGGTTGCTCAATATACACTCTATAGTGTATCAAGGCCAACAATTGCCAAACCTATTAAGCATTTTGACAGGATGGGGCAAGCACAGCAAAGTAGTAGGCGACAGTGCCTTGAGACGAGCAGTCGAGGCACTTCTCACCAGCATGGGTGCACCCTTTCGGGTTCACGAGTGTAACATAGGTAGGTTTATATCGACAGGCTCAGTGGCTGCGGCCTGGTTAAAAGAATCTGGCACACTGGAGGTGCTCATGCTTCATGATGATAGGGCTGAGCCAAACAGTGCAAATTTTGGCCAAATATCCGATTTACGAGCGCTCGCATTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0010019 GO:0016070 GO:0016787 GO:0016788 GO:0023052 GO:0031930 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3 Smr

Protein Analysis

684

Amino Acids

75.49

Weight (kDa)

6.23

Isoelectric Point (pI)

30.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_24	PF23276	188 - 313	5.7e-11	Fungal tetratrico peptide repeats
PPR_long	PF17177	201 - 302	2.7e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	204 - 264	3.5e-13	Pentatricopeptide repeat domain
PPR_1	PF12854	215 - 244	2.5e-06	PPR repeat
PPR_2	PF13041	216 - 264	1.5e-13	PPR repeat family
PPR	PF01535	218 - 248	5.3e-08	PPR repeat
PPR_long	PF17177	274 - 408	1.7e-10	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	289 - 334	1.4e-11	PPR repeat family
PPR	PF01535	289 - 319	1.4e-06	PPR repeat
PPR_3	PF13812	310 - 368	1.7e-12	Pentatricopeptide repeat domain
PPR_2	PF13041	322 - 370	2.9e-16	PPR repeat family
PPR_3	PF13812	344 - 405	1e-08	Pentatricopeptide repeat domain
PPR_1	PF12854	352 - 385	7.8e-14	PPR repeat
PPR_2	PF13041	356 - 386	2.4e-10	PPR repeat family
PPR	PF01535	359 - 385	1.3e-09	PPR repeat
PPR	PF01535	394 - 424	5.7e-07	PPR repeat
PPR_3	PF13812	415 - 474	1.2e-11	Pentatricopeptide repeat domain
PPR_2	PF13041	426 - 474	4.3e-15	PPR repeat family
PPR	PF01535	429 - 457	2.4e-07	PPR repeat
PPR_1	PF12854	459 - 488	7.2e-06	PPR repeat
PPR	PF01535	465 - 494	9.1e-06	PPR repeat
TPR_24	PF23276	476 - 545	1.9e-06	Fungal tetratrico peptide repeats
PPR_3	PF13812	484 - 544	1.2e-13	Pentatricopeptide repeat domain
PPR_1	PF12854	493 - 525	6.1e-10	PPR repeat
PPR_2	PF13041	497 - 544	6.5e-16	PPR repeat family
PPR	PF01535	499 - 529	5.7e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0010361)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31400 AT2G31400
fragaria_vesca	FvH4_1g27040 FvH4_1g27040 FvH4_1g27040 FvH4_1g27040 FvH4_1g27040
malus_domestica	MD09G1284400.v1.1 MD17G1277600.v1.1
prunus_persica	Prupe.3G009900_v2.0.a1 Prupe.3G010400_v2.0.a1 Prupe.3G010400_v2.0.a1
pyrus_communis	pycom09g19230 pycom17g27520
rosa_chinensis	RchiOBHm_Chr3g0487361
rosa_laevigata	RLG00000023011
rosa_multiflora	Rmu_sc0001955.1_g000008
rosa_roxburghii	Rroxscaffold_6G00394850
rosa_rugosa	Rorug03G0233500
rosa_samantha	Rh3DG313000
rosa_wichuraiana	Rw3G024920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	4 cut(s)	312, 417, 732, 1047
AatII	GACGTC	1 cut(s)	723
Acc36I	ACCTGC	1 cut(s)	651
AccB1I	GGYRCC	1 cut(s)	1106
AciI	CCGC	8 cut(s)	129, 209, 326, 356, 878, 1387, 1432, 1937
AcoI	YGGCCR	1 cut(s)	2017
AcsI	RAATTY	2 cut(s)	190, 2011
AcyI	GRCGYC	1 cut(s)	720
AfaI	GTAC	3 cut(s)	10, 526, 791
AfeI	AGCGCT	1 cut(s)	2040
AfiI	CCNNNNNNNGG	1 cut(s)	1071
AflIII	ACRYGT	1 cut(s)	811
AhdI	GACNNNNNGTC	1 cut(s)	1847
AjnI	CCWGG	2 cut(s)	1446, 1940
AjuI	GAANNNNNNNTTGG	2 cut(s)	176, 208
AluBI	AGCT	6 cut(s)	37, 655, 1247, 1256, 1367, 1508
AluI	AGCT	6 cut(s)	37, 655, 1247, 1256, 1367, 1508
Alw21I	GWGCWC	3 cut(s)	159, 1879, 1974
Alw26I	GTCTC	2 cut(s)	1518, 1834
Alw44I	GTGCAC	1 cut(s)	1875
AlwNI	CAGNNNCTG	2 cut(s)	1389, 1934
Ama87I	CYCGRG	2 cut(s)	158, 1710
Aor51HI	AGCGCT	1 cut(s)	2040
AoxI	GGCC	4 cut(s)	1073, 1762, 1938, 2017
ApaLI	GTGCAC	1 cut(s)	1875
ApeKI	GCWGC	6 cut(s)	126, 292, 1383, 1482, 1706, 1934
ApoI	RAATTY	2 cut(s)	190, 2011
ArsI	GACNNNNNNTTYG	2 cut(s)	1335, 1367
AseI	ATTAAT	1 cut(s)	954
AspLEI	GCGC	4 cut(s)	126, 1615, 1641, 2041
AsuHPI	GGTGA	4 cut(s)	223, 526, 810, 1855
AvaI	CYCGRG	2 cut(s)	158, 1710
BaeGI	GKGCMC	1 cut(s)	1879
BalI	TGGCCA	1 cut(s)	2019
BanI	GGYRCC	1 cut(s)	1106
BanII	GRGCYC	1 cut(s)	921
BauI	CACGAG	1 cut(s)	1892
BbsI	GAAGAC	1 cut(s)	1689
Bbv12I	GWGCWC	3 cut(s)	159, 1879, 1974
BbvI	GCAGC	6 cut(s)	138, 304, 1395, 1469, 1693, 1921
BccI	CCATC	6 cut(s)	373, 448, 530, 688, 1531, 1794
BcgI	CGANNNNNNTGC	2 cut(s)	281, 315
BciT130I	CCWGG	2 cut(s)	1448, 1942
BciVI	GTATCC	1 cut(s)	1204
BclI	TGATCA	1 cut(s)	397
BcoDI	GTCTC	2 cut(s)	1518, 1834
BfaI	CTAG	1 cut(s)	360
BfmI	CTRYAG	2 cut(s)	1483, 1749
BfoI	RGCGCY	2 cut(s)	1642, 2042
BfuAI	ACCTGC	1 cut(s)	651
BfuI	GTATCC	1 cut(s)	1204
BisI	GCNGC	9 cut(s)	127, 293, 357, 1384, 1387, 1483, 1707, 1935, 1938
BlpI	GCTNAGC	1 cut(s)	1995
BlsI	GCNGC	9 cut(s)	128, 294, 358, 1385, 1388, 1484, 1708, 1936, 1939
BmcAI	AGTACT	2 cut(s)	10, 526
Bme1390I	CCNGG	2 cut(s)	1448, 1942
BmeRI	GACNNNNNGTC	1 cut(s)	1847
BmeT110I	CYCGRG	2 cut(s)	158, 1710
BmiI	GGNNCC	1 cut(s)	1108
BmrFI	CCNGG	2 cut(s)	1448, 1942
BpiI	GAAGAC	1 cut(s)	1689
BpmI	CTGGAG	1 cut(s)	1985
Bpu1102I	GCTNAGC	1 cut(s)	1995
BpuEI	CTTGAG	2 cut(s)	905, 1858
BsaHI	GRCGYC	1 cut(s)	720
BsaJI	CCNNGG	3 cut(s)	337, 1014, 1465
Bsc4I	CCNNNNNNNGG	1 cut(s)	1071
Bse1I	ACTGG	1 cut(s)	1968
Bse3DI	GCAATG	2 cut(s)	632, 1722
BseBI	CCWGG	2 cut(s)	1448, 1942
BseDI	CCNNGG	3 cut(s)	337, 1014, 1465
BseGI	GGATG	3 cut(s)	436, 1023, 1805
BseLI	CCNNNNNNNGG	1 cut(s)	1071
BseMI	GCAATG	2 cut(s)	632, 1722
BseMII	CTCAG	3 cut(s)	1523, 1941, 1986
BseNI	ACTGG	1 cut(s)	1968
BseRI	GAGGAG	1 cut(s)	683
BseSI	GKGCMC	1 cut(s)	1879
BseXI	GCAGC	6 cut(s)	138, 304, 1395, 1469, 1693, 1921
BsgI	GTGCAG	2 cut(s)	311, 1113
BshFI	GGCC	4 cut(s)	1075, 1764, 1940, 2019
BshNI	GGYRCC	1 cut(s)	1106
BsiHKAI	GWGCWC	3 cut(s)	159, 1879, 1974
BsiHKCI	CYCGRG	2 cut(s)	158, 1710
BslFI	GGGAC	1 cut(s)	357
BslI	CCNNNNNNNGG	1 cut(s)	1071
BsmAI	GTCTC	2 cut(s)	1518, 1834
BsmBI	CGTCTC	1 cut(s)	1834
BsmFI	GGGAC	1 cut(s)	357
BsmI	GAATGC	1 cut(s)	1663
BsnI	GGCC	4 cut(s)	1075, 1764, 1940, 2019
BsoBI	CYCGRG	2 cut(s)	158, 1710
Bsp1286I	GDGCHC	4 cut(s)	159, 921, 1879, 1974
Bsp1407I	TGTACA	1 cut(s)	789
Bsp143I	GATC	2 cut(s)	298, 397
Bsp1720I	GCTNAGC	1 cut(s)	1995
Bsp19I	CCATGG	1 cut(s)	1465
BspACI	CCGC	8 cut(s)	129, 209, 326, 356, 878, 1387, 1432, 1937
BspANI	GGCC	4 cut(s)	1075, 1764, 1940, 2019
BspCNI	CTCAG	3 cut(s)	1522, 1940, 1987
BspHI	TCATGA	1 cut(s)	1981
BspLI	GGNNCC	1 cut(s)	1108
BspMAI	CTGCAG	1 cut(s)	1487
BspMI	ACCTGC	1 cut(s)	651
BspT107I	GGYRCC	1 cut(s)	1106
BsrDI	GCAATG	2 cut(s)	632, 1722
BsrGI	TGTACA	1 cut(s)	789
BsrI	ACTGG	1 cut(s)	1968
BssECI	CCNNGG	3 cut(s)	337, 1014, 1465
BssMI	GATC	2 cut(s)	298, 397
BssNI	GRCGYC	1 cut(s)	720
BssSI	CACGAG	1 cut(s)	1892
BssT1I	CCWWGG	2 cut(s)	1014, 1465
Bst2BI	CACGAG	1 cut(s)	1892
Bst2UI	CCWGG	2 cut(s)	1448, 1942
Bst4CI	ACNGT	8 cut(s)	88, 348, 524, 789, 1087, 1544, 1832, 2006
Bst6I	CTCTTC	1 cut(s)	1523
BstACI	GRCGYC	1 cut(s)	720
BstAPI	GCANNNNNTGC	1 cut(s)	1100
BstAUI	TGTACA	1 cut(s)	789
BstC8I	GCNNGC	6 cut(s)	39, 912, 1077, 1381, 1808, 2043
BstDEI	CTNAG	3 cut(s)	1509, 1927, 1995
BstDSI	CCRYGG	1 cut(s)	1465
BstENI	CCTNNNNNAGG	1 cut(s)	1069
BstF5I	GGATG	3 cut(s)	436, 1023, 1805
BstH2I	RGCGCY	2 cut(s)	1642, 2042
BstHHI	GCGC	4 cut(s)	126, 1615, 1641, 2041
BstKTI	GATC	2 cut(s)	301, 400
BstMAI	GTCTC	2 cut(s)	1518, 1834
BstMBI	GATC	2 cut(s)	298, 397
BstMWI	GCNNNNNNNGC	7 cut(s)	916, 1100, 1253, 1262, 1712, 1812, 1853
BstNI	CCWGG	2 cut(s)	1448, 1942
BstNSI	RCATGY	1 cut(s)	1585
BstSCI	CCNGG	2 cut(s)	1446, 1940
BstSFI	CTRYAG	2 cut(s)	1483, 1749
BstSLI	GKGCMC	1 cut(s)	1879
BstV1I	GCAGC	6 cut(s)	138, 304, 1395, 1469, 1693, 1921
BstV2I	GAAGAC	1 cut(s)	1689
BstXI	CCANNNNNNTGG	3 cut(s)	610, 1420, 1454
BsuI	GTATCC	1 cut(s)	1204
BsuRI	GGCC	4 cut(s)	1075, 1764, 1940, 2019
BtgI	CCRYGG	1 cut(s)	1465
BtsCI	GGATG	3 cut(s)	436, 1023, 1805
BtsI	GCAGTG	2 cut(s)	891, 1135
BtsIMutI	CAGTG	8 cut(s)	379, 891, 1092, 1135, 1837, 1935, 1961, 2011
BveI	ACCTGC	1 cut(s)	651
Cac8I	GCNNGC	6 cut(s)	39, 912, 1077, 1381, 1808, 2043
CaiI	CAGNNNCTG	2 cut(s)	1389, 1934
CciI	TCATGA	1 cut(s)	1981
CfoI	GCGC	4 cut(s)	126, 1615, 1641, 2041
CsiI	ACCWGGT	1 cut(s)	1446
Csp6I	GTAC	3 cut(s)	9, 525, 790
CviQI	GTAC	3 cut(s)	9, 525, 790
DdeI	CTNAG	3 cut(s)	1509, 1927, 1995
DpnI	GATC	2 cut(s)	300, 399
DpnII	GATC	2 cut(s)	298, 397
DriI	GACNNNNNGTC	1 cut(s)	1847
EaeI	YGGCCR	1 cut(s)	2017
Eam1104I	CTCTTC	1 cut(s)	1523
Eam1105I	GACNNNNNGTC	1 cut(s)	1847
EarI	CTCTTC	1 cut(s)	1523
Eco130I	CCWWGG	2 cut(s)	1014, 1465
Eco24I	GRGCYC	1 cut(s)	921
Eco47III	AGCGCT	1 cut(s)	2040
Eco88I	CYCGRG	2 cut(s)	158, 1710
EcoNI	CCTNNNNNAGG	1 cut(s)	1069
EcoRII	CCWGG	2 cut(s)	1446, 1940
EcoT14I	CCWWGG	2 cut(s)	1014, 1465
EcoT22I	ATGCAT	3 cut(s)	1329, 1663, 1729
EcoT38I	GRGCYC	1 cut(s)	921
ErhI	CCWWGG	2 cut(s)	1014, 1465
Esp3I	CGTCTC	1 cut(s)	1834
FaqI	GGGAC	1 cut(s)	357
FauI	CCCGC	1 cut(s)	122
FbaI	TGATCA	1 cut(s)	397
Fnu4HI	GCNGC	9 cut(s)	127, 293, 357, 1384, 1387, 1483, 1707, 1935, 1938
FokI	GGATG	3 cut(s)	443, 1010, 1812
FriOI	GRGCYC	1 cut(s)	921
Fsp4HI	GCNGC	9 cut(s)	127, 293, 357, 1384, 1387, 1483, 1707, 1935, 1938
FspBI	CTAG	1 cut(s)	360
GlaI	GCGC	4 cut(s)	125, 1614, 1640, 2040
GluI	GCNGC	9 cut(s)	127, 293, 357, 1384, 1387, 1483, 1707, 1935, 1938
GsuI	CTGGAG	1 cut(s)	1985
HaeII	RGCGCY	2 cut(s)	1642, 2042
HaeIII	GGCC	4 cut(s)	1075, 1764, 1940, 2019
HhaI	GCGC	4 cut(s)	126, 1615, 1641, 2041
Hin1I	GRCGYC	1 cut(s)	720
Hin6I	GCGC	4 cut(s)	124, 1613, 1639, 2039
HinP1I	GCGC	4 cut(s)	124, 1613, 1639, 2039
HindIII	AAGCTT	1 cut(s)	653
HinfI	GANTC	6 cut(s)	161, 173, 618, 983, 1133, 1952
HphI	GGTGA	4 cut(s)	223, 526, 810, 1855
Hpy166II	GTNNAC	3 cut(s)	947, 1877, 1891
Hpy188I	TCNGA	7 cut(s)	303, 481, 1028, 1174, 1286, 1320, 2029
Hpy188III	TCNNGA	5 cut(s)	401, 611, 1674, 1892, 1982
Hpy8I	GTNNAC	3 cut(s)	947, 1877, 1891
Hpy99I	CGWCG	1 cut(s)	725
HpyAV	CCTTC	8 cut(s)	22, 244, 498, 709, 883, 1013, 1273, 1625
HpyCH4III	ACNGT	8 cut(s)	88, 348, 524, 789, 1087, 1544, 1832, 2006
HpyCH4IV	ACGT	2 cut(s)	720, 811
HpyF10VI	GCNNNNNNNGC	7 cut(s)	916, 1100, 1253, 1262, 1712, 1812, 1853
HpyF3I	CTNAG	3 cut(s)	1509, 1927, 1995
HpySE526I	ACGT	2 cut(s)	720, 811
Hsp92I	GRCGYC	1 cut(s)	720
HspAI	GCGC	4 cut(s)	124, 1613, 1639, 2039
Ksp22I	TGATCA	1 cut(s)	397
Kzo9I	GATC	2 cut(s)	298, 397
LmnI	GCTCC	1 cut(s)	1636
Lsp1109I	GCAGC	6 cut(s)	138, 304, 1395, 1469, 1693, 1921
MabI	ACCWGGT	1 cut(s)	1446
MaeI	CTAG	1 cut(s)	360
MaeII	ACGT	2 cut(s)	720, 811
MaeIII	GTNAC	7 cut(s)	137, 196, 514, 639, 1039, 1354, 1898
MalI	GATC	2 cut(s)	300, 399
MboI	GATC	2 cut(s)	298, 397
MboII	GAAGA	9 cut(s)	567, 575, 1128, 1134, 1237, 1333, 1415, 1510, 1689
MfeI	CAATTG	2 cut(s)	1616, 1769
MhlI	GDGCHC	4 cut(s)	159, 921, 1879, 1974
MlsI	TGGCCA	1 cut(s)	2019
MluNI	TGGCCA	1 cut(s)	2019
MlyI	GAGTC	2 cut(s)	170, 627
MmeI	TCCRAC	2 cut(s)	459, 1051
Mox20I	TGGCCA	1 cut(s)	2019
Mph1103I	ATGCAT	3 cut(s)	1329, 1663, 1729
MscI	TGGCCA	1 cut(s)	2019
MseI	TTAA	8 cut(s)	246, 582, 780, 830, 954, 1530, 1784, 1946
MslI	CAYNNNNRTG	1 cut(s)	1868
Msp20I	TGGCCA	1 cut(s)	2019
MspA1I	CMGCKG	3 cut(s)	129, 1247, 1389
MspR9I	CCNGG	2 cut(s)	1448, 1942
MunI	CAATTG	2 cut(s)	1616, 1769
Mva1269I	GAATGC	1 cut(s)	1663
MvaI	CCWGG	2 cut(s)	1448, 1942
MwoI	GCNNNNNNNGC	7 cut(s)	916, 1100, 1253, 1262, 1712, 1812, 1853
NcoI	CCATGG	1 cut(s)	1465
NdeII	GATC	2 cut(s)	298, 397
NlaIV	GGNNCC	1 cut(s)	1108
NmeAIII	GCCGAG	1 cut(s)	362
NmuCI	GTSAC	5 cut(s)	137, 514, 639, 1039, 1354
NsiI	ATGCAT	3 cut(s)	1329, 1663, 1729
NspI	RCATGY	1 cut(s)	1585
PaeR7I	CTCGAG	1 cut(s)	158
PagI	TCATGA	1 cut(s)	1981
PctI	GAATGC	1 cut(s)	1663
PfeI	GAWTC	4 cut(s)	173, 983, 1133, 1952
PkrI	GCNGC	9 cut(s)	128, 294, 358, 1385, 1388, 1484, 1708, 1936, 1939
PleI	GAGTC	2 cut(s)	169, 626
PpsI	GAGTC	2 cut(s)	169, 626
PshBI	ATTAAT	1 cut(s)	954
PsiI	TTATAA	4 cut(s)	312, 417, 732, 1047
Psp6I	CCWGG	2 cut(s)	1446, 1940
PspGI	CCWGG	2 cut(s)	1446, 1940
PspN4I	GGNNCC	1 cut(s)	1108
PspXI	VCTCGAGB	1 cut(s)	158
PstI	CTGCAG	1 cut(s)	1487
PstNI	CAGNNNCTG	2 cut(s)	1389, 1934
PvuII	CAGCTG	1 cut(s)	1247
RsaI	GTAC	3 cut(s)	10, 526, 791
RsaNI	GTAC	3 cut(s)	9, 525, 790
RseI	CAYNNNNRTG	1 cut(s)	1868
SaqAI	TTAA	8 cut(s)	246, 582, 780, 830, 954, 1530, 1784, 1946
SatI	GCNGC	9 cut(s)	127, 293, 357, 1384, 1387, 1483, 1707, 1935, 1938
Sau3AI	GATC	2 cut(s)	298, 397
ScaI	AGTACT	2 cut(s)	10, 526
SchI	GAGTC	2 cut(s)	170, 627
ScrFI	CCNGG	2 cut(s)	1448, 1942
SduI	GDGCHC	4 cut(s)	159, 921, 1879, 1974
SexAI	ACCWGGT	1 cut(s)	1446
SfcI	CTRYAG	2 cut(s)	1483, 1749
Sfr274I	CTCGAG	1 cut(s)	158
SlaI	CTCGAG	1 cut(s)	158
SmiMI	CAYNNNNRTG	1 cut(s)	1868
SmlI	CTYRAG	3 cut(s)	158, 920, 1837
SmoI	CTYRAG	3 cut(s)	158, 920, 1837
SsiI	CCGC	8 cut(s)	129, 209, 326, 356, 878, 1387, 1432, 1937
SspI	AATATT	1 cut(s)	499
SspMI	CTAG	1 cut(s)	360
StyD4I	CCNGG	2 cut(s)	1446, 1940
StyI	CCWWGG	2 cut(s)	1014, 1465
TaaI	ACNGT	8 cut(s)	88, 348, 524, 789, 1087, 1544, 1832, 2006
TaiI	ACGT	2 cut(s)	723, 814
TaqI	TCGA	6 cut(s)	159, 267, 1335, 1521, 1851, 1919
TatI	WGTACW	3 cut(s)	8, 524, 789
TauI	GCSGC	3 cut(s)	359, 1389, 1940
TfiI	GAWTC	4 cut(s)	173, 983, 1133, 1952
Tru1I	TTAA	8 cut(s)	246, 582, 780, 830, 954, 1530, 1784, 1946
Tru9I	TTAA	8 cut(s)	246, 582, 780, 830, 954, 1530, 1784, 1946
TscAI	CASTG	8 cut(s)	379, 891, 1092, 1135, 1837, 1935, 1968, 2011
TseFI	GTSAC	5 cut(s)	137, 514, 639, 1039, 1354
TseI	GCWGC	6 cut(s)	126, 292, 1383, 1482, 1706, 1934
Tsp45I	GTSAC	5 cut(s)	137, 514, 639, 1039, 1354
TspRI	CASTG	8 cut(s)	379, 891, 1092, 1135, 1837, 1935, 1968, 2011
VneI	GTGCAC	1 cut(s)	1875
VspI	ATTAAT	1 cut(s)	954
XagI	CCTNNNNNAGG	1 cut(s)	1069
XapI	RAATTY	2 cut(s)	190, 2011
XceI	RCATGY	1 cut(s)	1585
XhoI	CTCGAG	1 cut(s)	158
XspI	CTAG	1 cut(s)	360
ZraI	GACGTC	1 cut(s)	721
ZrmI	AGTACT	2 cut(s)	10, 526
Zsp2I	ATGCAT	3 cut(s)	1329, 1663, 1729

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.