Rroxscaffold_6G00394850

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0010361
Physical Location & Seq
Reverse (-)
15704389 .. 15708121
3733 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00394850.1

Sequence Viewer

Length: 2460 bp
ATGGCGTCAACGCCGCCGCACTGTTCAATCACAGCGACCAAGCCGTACCAAACCCACCAATACCCACAAAACCAGCGCCTCAAATCTCAGCGGCAGACCCGTCAGAGCAACCAATGGACCACCCACCACGTCTCTCTCTCAAAGCCTCTACCTTTACCTCCCCAACCGCCGCGCACGGCGCCCAAACCCGCCGCCGCCGCCGCTCCCACTCCGACCTCCTTTTCCTCTCTCTGCCCTCGGCCCAAGTCCGACCTCGTCGCCGCCTTCTCGGGCCGCCGCTCGACCCGGTTCGTGTCCAAAATGCACTTGGGCCGGCCCAAAACCACGGTGGGGTCCCGCCACTCCTCGCTGGCGGAGGAGGCCCTGCAGAGCGCAATCCAGTTCGGTAAGGACGATTTGGCCCTTGATGATGTTTTGCTTAGTTTCGAGTCTAAGCTTTGTGGGTCGGATGATTACACTTTTTTGCTTAGAGAGCTTGGGAATAGAGGTGAGTGTTGGAAGGCTATAAGGTTGGGGAAGGTTGAGCTTGCCAAGAATGTGTTTCAGACTGCTGTCAATGAGGGTTATGGCAAGACTGTTTATACGTATTCGGCTTTGATTAGTGCTTATGGGCGCAGCGGGTATTGCGATGAGGCTATTCGAGTGCTCGAGTCCATGAAAGATTCTGGCTTGAAGCCGAATTTGGTTACTTACAATGCGGTGATTGATGCGTGTGGAAAAGGGGAGTGGAGTTTAAGAAGTCGAGGGGGACTGTGGGAGGCGGCTAGAAACTTGTTCAGTGAGATGGTGGATAGAGGGATTGATCAGGATATTTATACCTATAATACGCTTTTGGATGCAATTTCTAAAGGTGGGCAGATGGATTTGGCATATCAGATTATGTCGGAGATGCCTTCAAAGAATATTTTGCCTAATGTGGTGACTTATAGTACTATGATCGATGGTTATGCTAAGGCTGGTAGATTAGAAGATGCACTCAGTTTATTTAATGAAATGAAGTTTTTGGCCATTGGTCTGGATAGAGTTCTGTATAATACATTGATGTCACTTTACGGGAAGCTTGGCAGGTTTGCAGAGGCTTTGAATGTTTGCAAGGAGATGGAGAGTGTTGGGATTGTGAAGGACGTTGTATCTTATAATGCACTTCTTGGTGGGTATGGGAAGCAAGGCAAGTATGATCAAGTTAAAGGACTGTACAATGAAATGAAAGAAGAACGTGTATCACCAAATTTATTAACTTATTCAACATTGATTGATGTGTATTCAAAAGGTGGTTTGTATGTGGAGGCAATGAAAGTTTTCAGAGAGTTTAAGCAGGCAGGGCTGAAGGCTGATGTTGTGCTCTATAGTGAACTTATTAATGCTTTGTGCAAAAATGGGCTGGTGGAATCTGCTGTTTCATTGCTTGATGAGATGACCAAGGAAGGCATCCGACCTAATGTTGTCACTTATAATTCTATAATTGATGCCTTTGGTAGGCCAGCGACAACAGAGTGTGCAGTTGGTGCTGGTGCTGGAGGCATTGTGCCACACAATGAATCTTCATCTTCAATTTCTGAAAGGGATGTTGACATTTCTGAAGAGAATGTTCAAATTCAGGTGAGAGATTTGGAGGATACCAGAATTATAAAGATGTTTGGGCAGCTTGCAGCTGATAAAGCTGGTTATGCAACGAAAGATAGGAAGGTTAGACAAGAAATCTTGTGCATTTTGGGTGTCGTTCAGAAGATGCATGAGCTAGACATCAAACCGAATGTTGTCACATTTTCAGCCATTCTAAATGCTTGCAGCCGCTGTAATTCATTTGAAGATGCTTCCATGTTATTGGAGGAATTGCGGTTGTTTGATAACCAGGTTTATGGTGTTGCCCATGGACTTCTTATGGGCTACAGGGGTAATGTATGGGTGAAAGCTCAGTCCCTGTTTGATGAAGTTAAGCAGATGGACTGTTCAACTGCATCTGCATTCTATAATGCTCTTACCGACATGCTATGGCACTTTGGACAGAAACAAGGGGCGCAACTGGTTGTGCTTGAAGGGAAGCGCCGAAATGTATGGGAGAATGCATGGGAGGAGAATTCACAGTTAGATTTGCATTTGATGTCTTCTGGGGCTGCCCGAGCAATGGTTCATGCATGGTTGCTCAATATACACTCTATAGTGTATAAAGGCCAACAATTGCCAAACCTATTAAGCATTTTGACAGGATGGGGCAAGCACAGCAAAGTAGTAGGCGACAGTGCCTTAAGACGAGCAATCGAGGCACTTCTTACCAGCATGGGTGCACCCTTTCAGGTTGCCAAGCCTAACATAGGTAGGTTTATTTCGACGGGATCTGTGGCTGCTGCATGGTTAAAAGAATCTGGCACACTGGAGGTGCTCAAACTTCATGATGATAGGGCTGATCCAAATAGTGCAAATTTTGGCCAAATATCCAATTTACGAGCGCTTACTTTGTAG

Protein Analysis

819

Amino Acids

90.37

Weight (kDa)

8.22

Isoelectric Point (pI)

37.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_24 PF23276 169 - 238 2.6e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 180 - 240 6.3e-13 Pentatricopeptide repeat domain
PPR_2 PF13041 192 - 240 3.1e-13 PPR repeat family
PPR_1 PF12854 192 - 220 3.7e-06 PPR repeat
PPR PF01535 194 - 224 6.7e-08 PPR repeat
PPR_long PF17177 205 - 355 2.1e-11 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 250 - 282 5.1e-07 PPR repeat family
PPR_3 PF13812 258 - 314 2.1e-12 Pentatricopeptide repeat domain
PPR_2 PF13041 270 - 318 4.5e-16 PPR repeat family
PPR_3 PF13812 293 - 353 1.3e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 300 - 333 1.1e-13 PPR repeat
PPR_2 PF13041 304 - 334 2.5e-10 PPR repeat family
PPR PF01535 307 - 333 4.4e-10 PPR repeat
PPR PF01535 342 - 372 4.7e-06 PPR repeat
PPR_3 PF13812 363 - 422 1e-12 Pentatricopeptide repeat domain
PPR_2 PF13041 374 - 422 1.4e-15 PPR repeat family
PPR PF01535 377 - 406 4.3e-07 PPR repeat
PPR PF01535 413 - 442 3.1e-06 PPR repeat
TPR_24 PF23276 430 - 493 2e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 432 - 492 1.1e-13 Pentatricopeptide repeat domain
PPR_1 PF12854 441 - 473 5.7e-10 PPR repeat
PPR_2 PF13041 445 - 492 6.1e-16 PPR repeat family
PPR PF01535 447 - 477 5.4e-07 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 1137, 1452, 1628
Acc36I ACCTGC 1 cut(s) 1056
AccB1I GGYRCC 1 cut(s) 178
AccBSI CCGCTC 2 cut(s) 203, 279
AccII CGCG 1 cut(s) 172
AclWI GGATC 2 cut(s) 2341, 2399
AcoI YGGCCR 2 cut(s) 1005, 2425
AcsI RAATTY 5 cut(s) 679, 1228, 1593, 2077, 2419
AcuI CTGAAG 2 cut(s) 1346, 1599
AcyI GRCGYC 2 cut(s) 5, 179
AfaI GTAC 3 cut(s) 47, 931, 1196
AfeI AGCGCT 1 cut(s) 2448
AfiI CCNNNNNNNGG 4 cut(s) 330, 1476, 2125, 2312
AflII CTTAAG 1 cut(s) 2245
AflIII ACRYGT 1 cut(s) 1216
AjiI CACGTC 1 cut(s) 130
AjnI CCWGG 1 cut(s) 1851
AjuI GAANNNNNNNTTGG 2 cut(s) 665, 697
AluBI AGCT 9 cut(s) 436, 475, 526, 1060, 1645, 1652, 1661, 1738, 1913
AluI AGCT 9 cut(s) 436, 475, 526, 1060, 1645, 1652, 1661, 1738, 1913
Alw21I GWGCWC 4 cut(s) 648, 1344, 2287, 2382
Alw26I GTCTC 1 cut(s) 136
Alw44I GTGCAC 1 cut(s) 2283
AlwI GGATC 2 cut(s) 2341, 2399
AlwNI CAGNNNCTG 2 cut(s) 1794, 1921
Ama87I CYCGRG 3 cut(s) 268, 647, 2118
Aor51HI AGCGCT 1 cut(s) 2448
ApaLI GTGCAC 1 cut(s) 2283
ApeKI GCWGC 7 cut(s) 615, 1642, 1649, 1788, 2114, 2342, 2345
ApoI RAATTY 5 cut(s) 679, 1228, 1593, 2077, 2419
AseI ATTAAT 1 cut(s) 1359
Asp700I GAANNNNTTC 1 cut(s) 1298
AspLEI GCGC 8 cut(s) 78, 174, 181, 374, 615, 2020, 2046, 2449
AspS9I GGNCC 8 cut(s) 117, 240, 271, 310, 315, 333, 361, 400
AsuC2I CCSGG 1 cut(s) 286
AsuHPI GGTGA 6 cut(s) 500, 712, 931, 1215, 1612, 1918
AvaI CYCGRG 3 cut(s) 268, 647, 2118
AvaII GGWCC 2 cut(s) 117, 333
BaeGI GKGCMC 1 cut(s) 2287
BalI TGGCCA 2 cut(s) 1007, 2427
BanI GGYRCC 1 cut(s) 178
BbsI GAAGAC 1 cut(s) 2097
Bbv12I GWGCWC 4 cut(s) 648, 1344, 2287, 2382
BbvI GCAGC 7 cut(s) 627, 1654, 1661, 1800, 2101, 2329, 2332
BccI CCATC 6 cut(s) 778, 853, 935, 1093, 1936, 2202
BceAI ACGGC 2 cut(s) 28, 192
BcgI CGANNNNNNTGC 2 cut(s) 929, 963
BciT130I CCWGG 1 cut(s) 1853
BciVI GTATCC 1 cut(s) 1609
BclI TGATCA 2 cut(s) 802, 1177
BcnI CCSGG 1 cut(s) 286
BcoDI GTCTC 1 cut(s) 136
BfaI CTAG 2 cut(s) 765, 1739
BfmI CTRYAG 4 cut(s) 365, 1345, 1888, 2157
BfoI RGCGCY 4 cut(s) 79, 182, 2047, 2450
BfrI CTTAAG 1 cut(s) 2245
BfuAI ACCTGC 1 cut(s) 1056
BfuI GTATCC 1 cut(s) 1609
BmcAI AGTACT 1 cut(s) 931
Bme1390I CCNGG 2 cut(s) 286, 1853
Bme18I GGWCC 2 cut(s) 117, 333
BmeT110I CYCGRG 3 cut(s) 268, 647, 2118
BmgBI CACGTC 1 cut(s) 130
BmgT120I GGNCC 8 cut(s) 117, 240, 271, 310, 315, 333, 361, 400
BmiI GGNNCC 3 cut(s) 180, 334, 335
BmrFI CCNGG 2 cut(s) 286, 1853
BmsI GCATC 9 cut(s) 697, 826, 879, 961, 1437, 1456, 1719, 1801, 1967
BoxI GACNNNNGTC 1 cut(s) 551
BpiI GAAGAC 1 cut(s) 2097
BpmI CTGGAG 2 cut(s) 1536, 2393
Bpu10I CCTNAGC 1 cut(s) 951
BpuMI CCSGG 1 cut(s) 286
Bsa29I ATCGAT 1 cut(s) 939
BsaAI YACGTR 1 cut(s) 585
BsaHI GRCGYC 2 cut(s) 5, 179
BsaJI CCNNGG 4 cut(s) 236, 324, 1419, 1870
BsaXI ACNNNNNCTCC 2 cut(s) 1509, 1539
Bsc4I CCNNNNNNNGG 4 cut(s) 330, 1476, 2125, 2312
Bse118I RCCGGY 1 cut(s) 312
Bse1I ACTGG 3 cut(s) 379, 2028, 2376
Bse3DI GCAATG 3 cut(s) 1296, 1400, 2130
BseBI CCWGG 1 cut(s) 1853
BseCI ATCGAT 1 cut(s) 939
BseDI CCNNGG 4 cut(s) 236, 324, 1419, 1870
BseGI GGATG 5 cut(s) 454, 841, 1428, 1570, 2213
BseLI CCNNNNNNNGG 4 cut(s) 330, 1476, 2125, 2312
BseMI GCAATG 3 cut(s) 1296, 1400, 2130
BseMII CTCAG 3 cut(s) 101, 991, 1928
BseNI ACTGG 3 cut(s) 379, 2028, 2376
BseRI GAGGAG 3 cut(s) 334, 371, 2087
BseSI GKGCMC 1 cut(s) 2287
BseXI GCAGC 7 cut(s) 627, 1654, 1661, 1800, 2101, 2329, 2332
BsgI GTGCAG 1 cut(s) 1518
Bsh1236I CGCG 1 cut(s) 172
BshNI GGYRCC 1 cut(s) 178
BshVI ATCGAT 1 cut(s) 939
BsiHKAI GWGCWC 4 cut(s) 648, 1344, 2287, 2382
BsiHKCI CYCGRG 3 cut(s) 268, 647, 2118
BsiSI CCGG 2 cut(s) 286, 313
BslFI GGGAC 3 cut(s) 319, 762, 1903
BslI CCNNNNNNNGG 4 cut(s) 330, 1476, 2125, 2312
BsmAI GTCTC 1 cut(s) 136
BsmBI CGTCTC 1 cut(s) 136
BsmFI GGGAC 3 cut(s) 319, 762, 1903
BsmI GAATGC 2 cut(s) 1964, 2068
BsoBI CYCGRG 3 cut(s) 268, 647, 2118
Bsp1286I GDGCHC 4 cut(s) 648, 1344, 2287, 2382
Bsp1407I TGTACA 1 cut(s) 1194
Bsp143I GATC 5 cut(s) 802, 936, 1177, 2333, 2404
Bsp19I CCATGG 1 cut(s) 1870
BspCNI CTCAG 3 cut(s) 100, 990, 1927
BspDI ATCGAT 1 cut(s) 939
BspFNI CGCG 1 cut(s) 172
BspHI TCATGA 1 cut(s) 2389
BspLI GGNNCC 3 cut(s) 180, 334, 335
BspMAI CTGCAG 1 cut(s) 369
BspMI ACCTGC 1 cut(s) 1056
BspPI GGATC 2 cut(s) 2341, 2399
BspT107I GGYRCC 1 cut(s) 178
BspTI CTTAAG 1 cut(s) 2245
BsrBI CCGCTC 2 cut(s) 203, 279
BsrDI GCAATG 3 cut(s) 1296, 1400, 2130
BsrFI RCCGGY 1 cut(s) 312
BsrGI TGTACA 1 cut(s) 1194
BsrI ACTGG 3 cut(s) 379, 2028, 2376
BssAI RCCGGY 1 cut(s) 312
BssECI CCNNGG 4 cut(s) 236, 324, 1419, 1870
BssMI GATC 5 cut(s) 802, 936, 1177, 2333, 2404
BssNI GRCGYC 2 cut(s) 5, 179
BssT1I CCWWGG 2 cut(s) 1419, 1870
Bst2UI CCWGG 1 cut(s) 1853
Bst4CI ACNGT 8 cut(s) 23, 328, 577, 753, 1194, 1949, 2085, 2240
Bst6I CTCTTC 1 cut(s) 1575
BstACI GRCGYC 2 cut(s) 5, 179
BstAFI CTTAAG 1 cut(s) 2245
BstAPI GCANNNNNTGC 1 cut(s) 1505
BstAUI TGTACA 1 cut(s) 1194
BstBAI YACGTR 1 cut(s) 585
BstC8I GCNNGC 8 cut(s) 314, 351, 528, 1317, 1482, 1647, 1786, 2216
BstDEI CTNAG 7 cut(s) 87, 419, 432, 467, 951, 977, 1914
BstDSI CCRYGG 2 cut(s) 324, 1870
BstENI CCTNNNNNAGG 2 cut(s) 1474, 2310
BstF5I GGATG 5 cut(s) 454, 841, 1428, 1570, 2213
BstFNI CGCG 1 cut(s) 172
BstH2I RGCGCY 4 cut(s) 79, 182, 2047, 2450
BstHHI GCGC 8 cut(s) 78, 174, 181, 374, 615, 2020, 2046, 2449
BstKTI GATC 5 cut(s) 805, 939, 1180, 2336, 2407
BstMAI GTCTC 1 cut(s) 136
BstMBI GATC 5 cut(s) 802, 936, 1177, 2333, 2404
BstNI CCWGG 1 cut(s) 1853
BstNSI RCATGY 1 cut(s) 1990
BstPAI GACNNNNGTC 1 cut(s) 551
BstSCI CCNGG 2 cut(s) 284, 1851
BstSFI CTRYAG 4 cut(s) 365, 1345, 1888, 2157
BstSLI GKGCMC 1 cut(s) 2287
BstSNI TACGTA 1 cut(s) 585
BstUI CGCG 1 cut(s) 172
BstV1I GCAGC 7 cut(s) 627, 1654, 1661, 1800, 2101, 2329, 2332
BstV2I GAAGAC 1 cut(s) 2097
BstX2I RGATCY 1 cut(s) 2333
BstXI CCANNNNNNTGG 3 cut(s) 1015, 1825, 1859
BstYI RGATCY 1 cut(s) 2333
Bsu15I ATCGAT 1 cut(s) 939
BsuI GTATCC 1 cut(s) 1609
BsuTUI ATCGAT 1 cut(s) 939
BtgI CCRYGG 2 cut(s) 324, 1870
BtgZI GCGATG 1 cut(s) 642
BtrI CACGTC 1 cut(s) 130
BtsCI GGATG 5 cut(s) 454, 841, 1428, 1570, 2213
BtsIMutI CAGTG 4 cut(s) 19, 784, 2245, 2369
BveI ACCTGC 1 cut(s) 1056
Cac8I GCNNGC 8 cut(s) 314, 351, 528, 1317, 1482, 1647, 1786, 2216
CaiI CAGNNNCTG 2 cut(s) 1794, 1921
CciI TCATGA 1 cut(s) 2389
CfoI GCGC 8 cut(s) 78, 174, 181, 374, 615, 2020, 2046, 2449
Cfr10I RCCGGY 1 cut(s) 312
Cfr13I GGNCC 8 cut(s) 117, 240, 271, 310, 315, 333, 361, 400
ClaI ATCGAT 1 cut(s) 939
CsiI ACCWGGT 1 cut(s) 1851
Csp6I GTAC 3 cut(s) 46, 930, 1195
CviQI GTAC 3 cut(s) 46, 930, 1195
DdeI CTNAG 7 cut(s) 87, 419, 432, 467, 951, 977, 1914
DinI GGCGCC 1 cut(s) 180
DpnI GATC 5 cut(s) 804, 938, 1179, 2335, 2406
DpnII GATC 5 cut(s) 802, 936, 1177, 2333, 2404
EaeI YGGCCR 2 cut(s) 1005, 2425
Eam1104I CTCTTC 1 cut(s) 1575
EarI CTCTTC 1 cut(s) 1575
EciI GGCGGA 1 cut(s) 368
Eco105I TACGTA 1 cut(s) 585
Eco130I CCWWGG 2 cut(s) 1419, 1870
Eco47I GGWCC 2 cut(s) 117, 333
Eco47III AGCGCT 1 cut(s) 2448
Eco57I CTGAAG 2 cut(s) 1346, 1599
Eco88I CYCGRG 3 cut(s) 268, 647, 2118
EcoNI CCTNNNNNAGG 2 cut(s) 1474, 2310
EcoO109I RGGNCCY 2 cut(s) 333, 361
EcoRI GAATTC 1 cut(s) 2077
EcoRII CCWGG 1 cut(s) 1851
EcoT14I CCWWGG 2 cut(s) 1419, 1870
EcoT22I ATGCAT 3 cut(s) 1734, 2068, 2137
EgeI GGCGCC 1 cut(s) 180
EheI GGCGCC 1 cut(s) 180
ErhI CCWWGG 2 cut(s) 1419, 1870
Esp3I CGTCTC 1 cut(s) 136
FaqI GGGAC 3 cut(s) 319, 762, 1903
FauI CCCGC 3 cut(s) 196, 344, 611
FbaI TGATCA 2 cut(s) 802, 1177
FokI GGATG 5 cut(s) 461, 848, 1415, 1577, 2220
FseI GGCCGGCC 1 cut(s) 316
FspBI CTAG 2 cut(s) 765, 1739
GlaI GCGC 8 cut(s) 77, 173, 180, 373, 614, 2019, 2045, 2448
GsuI CTGGAG 2 cut(s) 1536, 2393
HaeII RGCGCY 4 cut(s) 79, 182, 2047, 2450
HapII CCGG 2 cut(s) 286, 313
HhaI GCGC 8 cut(s) 78, 174, 181, 374, 615, 2020, 2046, 2449
Hin1I GRCGYC 2 cut(s) 5, 179
Hin6I GCGC 8 cut(s) 76, 172, 179, 372, 613, 2018, 2044, 2447
HinP1I GCGC 8 cut(s) 76, 172, 179, 372, 613, 2018, 2044, 2447
HincII GTYRAC 2 cut(s) 9, 1570
HindII GTYRAC 2 cut(s) 9, 1570
HindIII AAGCTT 2 cut(s) 434, 1058
HinfI GANTC 6 cut(s) 428, 650, 662, 1388, 1538, 2360
HpaII CCGG 2 cut(s) 286, 313
HphI GGTGA 6 cut(s) 500, 712, 931, 1215, 1612, 1918
Hpy166II GTNNAC 4 cut(s) 9, 1352, 1570, 2285
Hpy188III TCNNGA 3 cut(s) 806, 1016, 2390
Hpy8I GTNNAC 4 cut(s) 9, 1352, 1570, 2285
Hpy99I CGWCG 2 cut(s) 260, 2332
HpyAV CCTTC 9 cut(s) 274, 493, 511, 903, 1114, 1321, 1418, 1678, 2030
HpyCH4III ACNGT 8 cut(s) 23, 328, 577, 753, 1194, 1949, 2085, 2240
HpyCH4IV ACGT 4 cut(s) 129, 584, 1125, 1216
HpyF3I CTNAG 7 cut(s) 87, 419, 432, 467, 951, 977, 1914
HpySE526I ACGT 4 cut(s) 129, 584, 1125, 1216
Hsp92I GRCGYC 2 cut(s) 5, 179
HspAI GCGC 8 cut(s) 76, 172, 179, 372, 613, 2018, 2044, 2447
KasI GGCGCC 1 cut(s) 178
KflI GGGWCCC 1 cut(s) 333
KroI GCCGGC 1 cut(s) 312
KroNI GCCGGC 1 cut(s) 314
Ksp22I TGATCA 2 cut(s) 802, 1177
Kzo9I GATC 5 cut(s) 802, 936, 1177, 2333, 2404
LmnI GCTCC 1 cut(s) 208
Lsp1109I GCAGC 7 cut(s) 627, 1654, 1661, 1800, 2101, 2329, 2332
LweI GCATC 9 cut(s) 697, 826, 879, 961, 1437, 1456, 1719, 1801, 1967
MabI ACCWGGT 1 cut(s) 1851
MaeI CTAG 2 cut(s) 765, 1739
MaeII ACGT 4 cut(s) 129, 584, 1125, 1216
MaeIII GTNAC 5 cut(s) 685, 919, 1044, 1444, 1759
MalI GATC 5 cut(s) 804, 938, 1179, 2335, 2406
MbiI CCGCTC 2 cut(s) 203, 279
MboI GATC 5 cut(s) 802, 936, 1177, 2333, 2404
MboII GAAGA 8 cut(s) 980, 1223, 1533, 1539, 1592, 1738, 1820, 2097
MfeI CAATTG 1 cut(s) 2177
MflI RGATCY 1 cut(s) 2333
MhlI GDGCHC 4 cut(s) 648, 1344, 2287, 2382
MlsI TGGCCA 2 cut(s) 1007, 2427
MluNI TGGCCA 2 cut(s) 1007, 2427
Mly113I GGCGCC 1 cut(s) 179
MlyI GAGTC 2 cut(s) 437, 659
MmeI TCCRAC 6 cut(s) 236, 273, 426, 476, 864, 1456
Mox20I TGGCCA 2 cut(s) 1007, 2427
Mph1103I ATGCAT 3 cut(s) 1734, 2068, 2137
MroNI GCCGGC 1 cut(s) 312
MroXI GAANNNNTTC 1 cut(s) 1298
MscI TGGCCA 2 cut(s) 1007, 2427
Msp20I TGGCCA 2 cut(s) 1007, 2427
MspA1I CMGCKG 4 cut(s) 91, 618, 1652, 1794
MspCI CTTAAG 1 cut(s) 2245
MspI CCGG 2 cut(s) 286, 313
MspR9I CCNGG 2 cut(s) 286, 1853
MunI CAATTG 1 cut(s) 2177
Mva1269I GAATGC 2 cut(s) 1964, 2068
MvaI CCWGG 1 cut(s) 1853
MvnI CGCG 1 cut(s) 172
NaeI GCCGGC 1 cut(s) 314
NarI GGCGCC 1 cut(s) 179
NciI CCSGG 1 cut(s) 286
NcoI CCATGG 1 cut(s) 1870
NdeII GATC 5 cut(s) 802, 936, 1177, 2333, 2404
NgoMIV GCCGGC 1 cut(s) 312
NlaIV GGNNCC 3 cut(s) 180, 334, 335
NmeAIII GCCGAG 1 cut(s) 217
NmuCI GTSAC 4 cut(s) 919, 1044, 1444, 1759
NsiI ATGCAT 3 cut(s) 1734, 2068, 2137
NspI RCATGY 1 cut(s) 1990
PaeR7I CTCGAG 1 cut(s) 647
PagI TCATGA 1 cut(s) 2389
PcsI WCGNNNNNNNCGW 1 cut(s) 390
PctI GAATGC 2 cut(s) 1964, 2068
PdiI GCCGGC 1 cut(s) 314
PdmI GAANNNNTTC 1 cut(s) 1298
PfeI GAWTC 4 cut(s) 662, 1388, 1538, 2360
PflFI GACNNNGTC 1 cut(s) 254
PleI GAGTC 2 cut(s) 436, 658
PluTI GGCGCC 1 cut(s) 182
PpsI GAGTC 2 cut(s) 436, 658
Ppu21I YACGTR 1 cut(s) 585
PpuMI RGGWCCY 1 cut(s) 333
PshAI GACNNNNGTC 1 cut(s) 551
PshBI ATTAAT 1 cut(s) 1359
PsiI TTATAA 3 cut(s) 1137, 1452, 1628
Psp5II RGGWCCY 1 cut(s) 333
Psp6I CCWGG 1 cut(s) 1851
PspGI CCWGG 1 cut(s) 1851
PspN4I GGNNCC 3 cut(s) 180, 334, 335
PspPI GGNCC 8 cut(s) 117, 240, 271, 310, 315, 333, 361, 400
PspPPI RGGWCCY 1 cut(s) 333
PspXI VCTCGAGB 1 cut(s) 647
PstI CTGCAG 1 cut(s) 369
PstNI CAGNNNCTG 2 cut(s) 1794, 1921
PsuI RGATCY 1 cut(s) 2333
PsyI GACNNNGTC 1 cut(s) 254
PvuII CAGCTG 1 cut(s) 1652
RigI GGCCGGCC 1 cut(s) 316
RsaI GTAC 3 cut(s) 47, 931, 1196
RsaNI GTAC 3 cut(s) 46, 930, 1195
Sau3AI GATC 5 cut(s) 802, 936, 1177, 2333, 2404
Sau96I GGNCC 8 cut(s) 117, 240, 271, 310, 315, 333, 361, 400
ScaI AGTACT 1 cut(s) 931
SchI GAGTC 2 cut(s) 437, 659
ScrFI CCNGG 2 cut(s) 286, 1853
SduI GDGCHC 4 cut(s) 648, 1344, 2287, 2382
SexAI ACCWGGT 1 cut(s) 1851
SfaNI GCATC 9 cut(s) 697, 826, 879, 961, 1437, 1456, 1719, 1801, 1967
SfcI CTRYAG 4 cut(s) 365, 1345, 1888, 2157
SfoI GGCGCC 1 cut(s) 180
Sfr274I CTCGAG 1 cut(s) 647
SinI GGWCC 2 cut(s) 117, 333
SlaI CTCGAG 1 cut(s) 647
SmlI CTYRAG 2 cut(s) 647, 2245
SmoI CTYRAG 2 cut(s) 647, 2245
SnaBI TACGTA 1 cut(s) 585
SspDI GGCGCC 1 cut(s) 178
SspI AATATT 1 cut(s) 904
SspMI CTAG 2 cut(s) 765, 1739
StyD4I CCNGG 2 cut(s) 284, 1851
StyI CCWWGG 2 cut(s) 1419, 1870
TaaI ACNGT 8 cut(s) 23, 328, 577, 753, 1194, 1949, 2085, 2240
TaiI ACGT 4 cut(s) 132, 587, 1128, 1219
TaqI TCGA 8 cut(s) 281, 426, 640, 648, 742, 939, 2259, 2327
TatI WGTACW 2 cut(s) 929, 1194
TfiI GAWTC 4 cut(s) 662, 1388, 1538, 2360
TscAI CASTG 4 cut(s) 26, 784, 2245, 2376
TseFI GTSAC 4 cut(s) 919, 1044, 1444, 1759
TseI GCWGC 7 cut(s) 615, 1642, 1649, 1788, 2114, 2342, 2345
Tsp45I GTSAC 4 cut(s) 919, 1044, 1444, 1759
TspRI CASTG 4 cut(s) 26, 784, 2245, 2376
Tth111I GACNNNGTC 1 cut(s) 254
Vha464I CTTAAG 1 cut(s) 2245
VneI GTGCAC 1 cut(s) 2283
VpaK11BI GGWCC 2 cut(s) 117, 333
VspI ATTAAT 1 cut(s) 1359
XagI CCTNNNNNAGG 2 cut(s) 1474, 2310
XapI RAATTY 5 cut(s) 679, 1228, 1593, 2077, 2419
XceI RCATGY 1 cut(s) 1990
XcmI CCANNNNNNNNNTGG 2 cut(s) 304, 325
XhoI CTCGAG 1 cut(s) 647
XmnI GAANNNNTTC 1 cut(s) 1298
XspI CTAG 2 cut(s) 765, 1739
ZrmI AGTACT 1 cut(s) 931
Zsp2I ATGCAT 3 cut(s) 1734, 2068, 2137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.