Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr09

Orthogroup ID

OG0010361

Physical Location & Seq

Genomic Coordinates Reverse (-)

36211254 .. 36215239

Gene Structure 3986 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD09G1284400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2607 bp

ATGGCTTCAACGCCGCCGCACTGTTCAATCACGGCGACTAAGCCTTACCAGAGCCACCAATACCCCCAAAACCAGCGCCTAAAATCTCAGCGCAACGCCCGCCAACCCAATCAATGGACGACCCAACAAGTCTCCCTCCCAAAGCCTCTGCCTTTACCTTCCCAACCACCGCGCTCCGCCCCAAGGCCGCCGCTTCCCGCCCCCACCTCCTCCTTTTCATCCCTCCCTTCTCTCCCTCCTCCAAAATCTGATCTTGTCACCGCCTTCTCGGGCCGCCGCTCCACCCGATTCGTCTCCAAAATGCACCTGGGCCGCCCCAAGACCACCGTGGGCTCCCGCCACTCGGCGCTGGCAGAGGAGGCCCTGCACCACGCCGTCCAATACGGTAAGAATGATCTGGCTCTTGATGATGTTTTGCTTAGTTTTGAGACTAGGCTTGGTGGGTCTGATGATTACACGTTTTTGCTAAGAGAGCTTGGGAATAGGGGTGAGTGTTGGAAGGCAATTCGATGTTTCGAGTTTGCGGTGAGGAGGGAGAGGAGGAGAACTGAGCAGGGGAAATTAGCTAGTTCTATGATTAGTACTCTTGGCAGGTTAGGAAAGGTTGAGCTTGCTAAGAATGTGTTTCAAACTGCTGTTGATGACGGCTACGGGAAGACCGTTTATACGTATTCTGCGTTGATTAGTGCTTATGGGCGAAGTGGGTATTGTGAGGAGGCTATAAGAGTTCTTGAGTCAATGAAAGATGCGGGCTTGAAACCGAATTTGGTTACTTACAATGCGGTAATTGATGCGTGTGGAAAAGGGGGAGTGGAATTTAAGAGGGTAGTGGAGATTTTCAACGAGATGCTGAGAAATGGGGTTCAGCCAGATCGAATTACTTATAATTCACTCCTTGCGGTTTGTAGTCGAGGGGGATTGTGGGAGGCAGCTCAAAACCTATTTAGTGAGATGGTGGATAGAGGAATTGATCAGGATATTTACACGTACAATACGCTTCTGGATGCGATTTGTAAAGGTGGGCAGATGGATTTGGCATATCAGATGATGTCGGAGATGCCTTCAAAGAATATTTTGCCCAATGTGGTTACTTATAGTACTATTATCGATGGATATGCTAAGGCTGGTAGATTAGAAGATGCACTCAGTTTATTTAATGAAATGAAGTTTTTGGCCATCAGTCTGGACAGAGTTCTGTATAACACATTGCTTTCACTATATGGGAAGCTTGGTAGGTTTGAGGACGCCTTGAATGTTTGCAAGGAGATGGAGAGTGTTGGGATTGCGAAGGATGTTGTGTCTTATAATGCACTTCTTGGTGGGTATGGGAAGCAAGGGAAGTATGATGAAGTTAAAAGAATGTACAATGAGATGATAGAAGAACATGTATCACCAAACATATTAACTTATTCAACATTGATCGATCTGTATTCAAAGGGTGGTTTGTATGCAGAGGCAATGAGGGTTTTTAGAGAGTTTAAGCATGCAGGGTTGAAGGCTGATGTTGTGCTTTATAGTGAACTTATCAATGCTCTGTGCAAAAATGGACTGGTGGAATCTGCTCTTTCGTTGCTTGATGAGATGACCAAGGAAGGGATTCGACCTAATGTTGTCACTTATAATTCAATAATTGATGCCTTTGGTCGGTCAGCAGCTACAGAATGTGCAGTGGATGCTGCTCCAGGAGGCATTATGCTACAGACCGGATCTTCATCTACAGTTTCTGAAAGGAAGGTCTTTGAAATTCAGGTGGGAGATAGGGAGGATAACAGATTTCTGAAGATGTTTGGGCAACTTGCAGCTGAGAAAGCAGGTTATGCAATAAAAGATAGGAAGGTTAGACAAGAAATCTTGTGCATTCTGGGAATCTTTCAGAAGATGCATGAGCTAGACATCAAGCCGAATGTTGTCACATTTTCTGCCATTTTAAATGCGTGCAGCCGTTGTAATTCATTTGACGATGCTTCAATGTTATTGGAGGAACTCCGGTTGTTCGATAATCAAGTTTATGGGGTTGCCCACGGACTTCTTATGGGCTACCGGGATAATGTATGGGTTAAAGCTCAGTCTCTGTTTGATGAAGTTAAGCAGATGGACTCTTCGACTGCATCTGCATTCTATAATGCTCTGACCGACATGCTATGGCACTTCGGACAGAAACAAGGGGCACAACTGGTTGTGCTTGAAGGGAAACGCCGAAATGTATGGGAAAGTGTGTGGTCTGATTCGTGCTTAGATTTGCATCTAATGTCTCCTGGGGCTGCCCGGGCAATGGTTCATGCATGGTTGCTCAATATACGCACTATAGTGTTTAAAGGCCAACAATTACCGAACCTGTTAAGCATTCTAACAGGATGGGGGAAGCACAGCAAAGTAGTAGGTGACAGTACCTTACGACGAGCTATTGAGGCACTTCTTACTAGCATGGGTGCACCCTTTCACATTGCCAAGTGTAACTTGGGTAGGTTTATATCGACGGGCTCTGTGGCCGCTGCCTGGTTAAAAGAATCTGGGACACTGGAGGTGCTCGTTCTTCACGATGACAGGACTTACCCAAAAGGTGCACATTTGGACCAAATCTCCAATTTTCAAGCGCTAGCCTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006139 GO:0006725 GO:0006807 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0009451 GO:0009507 GO:0009536 GO:0009987 GO:0010019 GO:0016070 GO:0016787 GO:0016788 GO:0023052 GO:0031930 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0090304 GO:0090305 GO:0097159 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PPR PPR_1 PPR_2 PPR_3 Smr

Protein Analysis

869

Amino Acids

96.39

Weight (kDa)

8.5

Isoelectric Point (pI)

42.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TPR_24	PF23276	192 - 287	1.5e-09	Fungal tetratrico peptide repeats
PPR_3	PF13812	208 - 268	4.8e-13	Pentatricopeptide repeat domain
PPR_2	PF13041	220 - 268	4.8e-13	PPR repeat family
PPR	PF01535	222 - 252	1.2e-07	PPR repeat
PPR_long	PF17177	233 - 377	2.8e-12	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	273 - 303	5.2e-06	PPR repeat family
PPR_long	PF17177	275 - 414	3.5e-11	Pentacotripeptide-repeat region of PRORP
PPR_2	PF13041	290 - 339	9.5e-14	PPR repeat family
PPR	PF01535	293 - 323	2.1e-06	PPR repeat
PPR_3	PF13812	314 - 372	1.5e-12	Pentatricopeptide repeat domain
PPR_1	PF12854	322 - 353	1.4e-07	PPR repeat
PPR_2	PF13041	326 - 374	7.3e-17	PPR repeat family
PPR_long	PF17177	344 - 451	6.1e-06	Pentacotripeptide-repeat region of PRORP
PPR_3	PF13812	350 - 409	6.6e-09	Pentatricopeptide repeat domain
PPR_1	PF12854	356 - 389	1.4e-13	PPR repeat
PPR_2	PF13041	360 - 396	1.3e-10	PPR repeat family
PPR	PF01535	363 - 389	3.7e-09	PPR repeat
PPR_3	PF13812	383 - 444	5.6e-09	Pentatricopeptide repeat domain
PPR	PF01535	398 - 428	2.1e-06	PPR repeat
PPR_3	PF13812	419 - 478	4.5e-12	Pentatricopeptide repeat domain
PPR_2	PF13041	430 - 477	6.2e-15	PPR repeat family
PPR	PF01535	433 - 461	1.8e-07	PPR repeat
PPR_long	PF17177	454 - 557	9.9e-06	Pentacotripeptide-repeat region of PRORP
PPR_1	PF12854	463 - 492	1e-05	PPR repeat
TPR_24	PF23276	484 - 552	4.7e-07	Fungal tetratrico peptide repeats
PPR_3	PF13812	488 - 548	5.9e-14	Pentatricopeptide repeat domain
PPR_1	PF12854	497 - 529	1e-09	PPR repeat
PPR_2	PF13041	501 - 548	5.4e-16	PPR repeat family
PPR	PF01535	503 - 533	3.6e-07	PPR repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0010361)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G31400 AT2G31400
fragaria_vesca	FvH4_1g27040 FvH4_1g27040 FvH4_1g27040 FvH4_1g27040 FvH4_1g27040
malus_domestica	MD09G1284400.v1.1 MD17G1277600.v1.1
prunus_persica	Prupe.3G009900_v2.0.a1 Prupe.3G010400_v2.0.a1 Prupe.3G010400_v2.0.a1
pyrus_communis	pycom09g19230 pycom17g27520
rosa_chinensis	RchiOBHm_Chr3g0487361
rosa_laevigata	RLG00000023011
rosa_multiflora	Rmu_sc0001955.1_g000008
rosa_roxburghii	Rroxscaffold_6G00394850
rosa_rugosa	Rorug03G0233500
rosa_samantha	Rh3DG313000
rosa_wichuraiana	Rw3G024920

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	885, 1305, 1620
Acc36I	ACCTGC	2 cut(s)	582, 1802
AccB7I	CCANNNNNTGG	1 cut(s)	114
AccBSI	CCGCTC	1 cut(s)	279
AccII	CGCG	1 cut(s)	172
AclWI	GGATC	1 cut(s)	1714
AcoI	YGGCCR	2 cut(s)	1173, 2487
AcsI	RAATTY	3 cut(s)	763, 815, 1743
AcuI	CTGAAG	1 cut(s)	1799
AcyI	GRCGYC	1 cut(s)	1245
AfaI	GTAC	5 cut(s)	583, 989, 1099, 1364, 2389
AfeI	AGCGCT	1 cut(s)	2595
AfiI	CCNNNNNNNGG	7 cut(s)	114, 183, 343, 1593, 1644, 2272, 2496
AflIII	ACRYGT	3 cut(s)	456, 984, 1384
AjnI	CCWGG	4 cut(s)	306, 1681, 2254, 2495
AjuI	GAANNNNNNNTTGG	2 cut(s)	749, 781
AleI	CACNNNNGTG	1 cut(s)	2306
Alw21I	GWGCWC	3 cut(s)	2434, 2529, 2566
Alw26I	GTCTC	5 cut(s)	136, 298, 422, 2073, 2256
Alw44I	GTGCAC	2 cut(s)	2430, 2562
AlwI	GGATC	1 cut(s)	1714
AlwNI	CAGNNNCTG	2 cut(s)	1724, 2071
Ama87I	CYCGRG	2 cut(s)	268, 2265
Aor51HI	AGCGCT	1 cut(s)	2595
AoxI	GGCC	7 cut(s)	185, 271, 310, 360, 1173, 2317, 2487
ApaLI	GTGCAC	2 cut(s)	2430, 2562
ApeKI	GCWGC	7 cut(s)	929, 1652, 1676, 1799, 1938, 2261, 2492
ApoI	RAATTY	3 cut(s)	763, 815, 1743
Asp700I	GAANNNNTTC	1 cut(s)	1596
AspLEI	GCGC	5 cut(s)	78, 93, 174, 349, 2596
AspS9I	GGNCC	4 cut(s)	271, 310, 361, 2572
AsuC2I	CCSGG	3 cut(s)	2042, 2266, 2267
AsuHPI	GGTGA	5 cut(s)	250, 500, 538, 1383, 2393
AsuNHI	GCTAGC	1 cut(s)	2596
AvaI	CYCGRG	2 cut(s)	268, 2265
AvaII	GGWCC	1 cut(s)	2572
BaeGI	GKGCMC	3 cut(s)	2170, 2434, 2566
BalI	TGGCCA	1 cut(s)	1175
BanII	GRGCYC	2 cut(s)	335, 2483
BbsI	GAAGAC	1 cut(s)	662
Bbv12I	GWGCWC	3 cut(s)	2434, 2529, 2566
BbvI	GCAGC	7 cut(s)	941, 1663, 1664, 1811, 1950, 2248, 2479
BccI	CCATC	7 cut(s)	946, 1021, 1103, 1184, 1261, 2086, 2349
BceAI	ACGGC	4 cut(s)	48, 359, 661, 1926
BcgI	CGANNNNNNTGC	2 cut(s)	1097, 1131
BciT130I	CCWGG	4 cut(s)	308, 1683, 2256, 2497
BclI	TGATCA	1 cut(s)	970
BcnI	CCSGG	3 cut(s)	2042, 2266, 2267
BcoDI	GTCTC	5 cut(s)	136, 298, 422, 2073, 2256
BfaI	CTAG	5 cut(s)	432, 567, 1889, 2421, 2597
BfmI	CTRYAG	4 cut(s)	1656, 1697, 1716, 2304
BfoI	RGCGCY	3 cut(s)	79, 350, 2597
BfuAI	ACCTGC	2 cut(s)	582, 1802
BmcAI	AGTACT	2 cut(s)	583, 1099
Bme1390I	CCNGG	7 cut(s)	308, 1683, 2042, 2256, 2266, 2267, 2497
Bme18I	GGWCC	1 cut(s)	2572
BmeT110I	CYCGRG	2 cut(s)	268, 2265
BmgT120I	GGNCC	4 cut(s)	271, 310, 361, 2572
BmiI	GGNNCC	1 cut(s)	334
BmrFI	CCNGG	7 cut(s)	308, 1683, 2042, 2256, 2266, 2267, 2497
BmtI	GCTAGC	1 cut(s)	2600
BpiI	GAAGAC	1 cut(s)	662
BpmI	CTGGAG	2 cut(s)	1665, 2540
Bpu10I	CCTNAGC	1 cut(s)	1119
BpuEI	CTTGAG	1 cut(s)	752
BpuMI	CCSGG	3 cut(s)	2042, 2266, 2267
Bsa29I	ATCGAT	2 cut(s)	1107, 1422
BsaAI	YACGTR	2 cut(s)	669, 987
BsaBI	GATNNNNATC	2 cut(s)	1711, 2241
BsaHI	GRCGYC	1 cut(s)	1245
BsaJI	CCNNGG	7 cut(s)	182, 307, 327, 1587, 2020, 2255, 2265
BsaWI	WCCGGW	2 cut(s)	1703, 1986
BsaXI	ACNNNNNCTCC	2 cut(s)	2564, 2594
Bsc4I	CCNNNNNNNGG	7 cut(s)	114, 183, 343, 1593, 1644, 2272, 2496
Bse1I	ACTGG	3 cut(s)	1554, 2178, 2523
Bse3DI	GCAATG	4 cut(s)	1205, 1464, 2277, 2442
Bse8I	GATNNNNATC	2 cut(s)	1711, 2241
BseBI	CCWGG	4 cut(s)	308, 1683, 2256, 2497
BseCI	ATCGAT	2 cut(s)	1107, 1422
BseDI	CCNNGG	7 cut(s)	182, 307, 327, 1587, 2020, 2255, 2265
BseGI	GGATG	5 cut(s)	218, 1009, 1297, 1678, 2360
BseJI	GATNNNNATC	2 cut(s)	1711, 2241
BseLI	CCNNNNNNNGG	7 cut(s)	114, 183, 343, 1593, 1644, 2272, 2496
BseMI	GCAATG	4 cut(s)	1205, 1464, 2277, 2442
BseMII	CTCAG	6 cut(s)	101, 540, 842, 1159, 1794, 2078
BseNI	ACTGG	3 cut(s)	1554, 2178, 2523
BseRI	GAGGAG	7 cut(s)	199, 228, 371, 544, 553, 556, 728
BseSI	GKGCMC	3 cut(s)	2170, 2434, 2566
BseXI	GCAGC	7 cut(s)	941, 1663, 1664, 1811, 1950, 2248, 2479
BsgI	GTGCAG	3 cut(s)	350, 1686, 1957
Bsh1236I	CGCG	1 cut(s)	172
BshFI	GGCC	7 cut(s)	187, 273, 312, 362, 1175, 2319, 2489
BshVI	ATCGAT	2 cut(s)	1107, 1422
BsiHKAI	GWGCWC	3 cut(s)	2434, 2529, 2566
BsiHKCI	CYCGRG	2 cut(s)	268, 2265
BsiSI	CCGG	4 cut(s)	1704, 1987, 2041, 2266
BslFI	GGGAC	1 cut(s)	2527
BslI	CCNNNNNNNGG	7 cut(s)	114, 183, 343, 1593, 1644, 2272, 2496
BsmAI	GTCTC	5 cut(s)	136, 298, 422, 2073, 2256
BsmBI	CGTCTC	1 cut(s)	298
BsmFI	GGGAC	1 cut(s)	2527
BsmI	GAATGC	3 cut(s)	1857, 2114, 2343
BsnI	GGCC	7 cut(s)	187, 273, 312, 362, 1175, 2319, 2489
BsoBI	CYCGRG	2 cut(s)	268, 2265
Bsp1286I	GDGCHC	6 cut(s)	335, 2170, 2434, 2483, 2529, 2566
Bsp1407I	TGTACA	1 cut(s)	1362
Bsp143I	GATC	7 cut(s)	250, 394, 871, 970, 1419, 1423, 1706
BspANI	GGCC	7 cut(s)	187, 273, 312, 362, 1175, 2319, 2489
BspCNI	CTCAG	6 cut(s)	100, 541, 843, 1158, 1795, 2077
BspDI	ATCGAT	2 cut(s)	1107, 1422
BspFNI	CGCG	1 cut(s)	172
BspLI	GGNNCC	1 cut(s)	334
BspMI	ACCTGC	2 cut(s)	582, 1802
BspOI	GCTAGC	1 cut(s)	2600
BspPI	GGATC	1 cut(s)	1714
BsrBI	CCGCTC	1 cut(s)	279
BsrDI	GCAATG	4 cut(s)	1205, 1464, 2277, 2442
BsrGI	TGTACA	1 cut(s)	1362
BsrI	ACTGG	3 cut(s)	1554, 2178, 2523
BssECI	CCNNGG	7 cut(s)	182, 307, 327, 1587, 2020, 2255, 2265
BssMI	GATC	7 cut(s)	250, 394, 871, 970, 1419, 1423, 1706
BssNI	GRCGYC	1 cut(s)	1245
BssT1I	CCWWGG	2 cut(s)	182, 1587
Bst2UI	CCWGG	4 cut(s)	308, 1683, 2256, 2497
Bst4CI	ACNGT	6 cut(s)	23, 328, 386, 661, 1720, 2387
Bst6I	CTCTTC	1 cut(s)	2104
BstACI	GRCGYC	1 cut(s)	1245
BstAPI	GCANNNNNTGC	2 cut(s)	1673, 1817
BstAUI	TGTACA	1 cut(s)	1362
BstBAI	YACGTR	2 cut(s)	669, 987
BstC8I	GCNNGC	7 cut(s)	100, 351, 612, 751, 1485, 1936, 2598
BstDSI	CCRYGG	2 cut(s)	327, 2020
BstF5I	GGATG	5 cut(s)	218, 1009, 1297, 1678, 2360
BstFNI	CGCG	1 cut(s)	172
BstH2I	RGCGCY	3 cut(s)	79, 350, 2597
BstHHI	GCGC	5 cut(s)	78, 93, 174, 349, 2596
BstKTI	GATC	7 cut(s)	253, 397, 874, 973, 1422, 1426, 1709
BstMAI	GTCTC	5 cut(s)	136, 298, 422, 2073, 2256
BstMBI	GATC	7 cut(s)	250, 394, 871, 970, 1419, 1423, 1706
BstMWI	GCNNNNNNNGC	8 cut(s)	99, 359, 472, 1673, 1808, 1817, 2267, 2408
BstNI	CCWGG	4 cut(s)	308, 1683, 2256, 2497
BstNSI	RCATGY	3 cut(s)	1388, 1487, 2140
BstSCI	CCNGG	7 cut(s)	306, 1681, 2040, 2254, 2264, 2265, 2495
BstSFI	CTRYAG	4 cut(s)	1656, 1697, 1716, 2304
BstSLI	GKGCMC	3 cut(s)	2170, 2434, 2566
BstSNI	TACGTA	1 cut(s)	669
BstUI	CGCG	1 cut(s)	172
BstV1I	GCAGC	7 cut(s)	941, 1663, 1664, 1811, 1950, 2248, 2479
BstV2I	GAAGAC	1 cut(s)	662
BstX2I	RGATCY	1 cut(s)	1706
BstXI	CCANNNNNNTGG	1 cut(s)	1183
BstYI	RGATCY	1 cut(s)	1706
Bsu15I	ATCGAT	2 cut(s)	1107, 1422
BsuRI	GGCC	7 cut(s)	187, 273, 312, 362, 1175, 2319, 2489
BsuTUI	ATCGAT	2 cut(s)	1107, 1422
BtgI	CCRYGG	2 cut(s)	327, 2020
BtsCI	GGATG	5 cut(s)	218, 1009, 1297, 1678, 2360
BtsI	GCAGTG	1 cut(s)	1674
BtsIMutI	CAGTG	3 cut(s)	19, 1674, 2516
BveI	ACCTGC	2 cut(s)	582, 1802
Cac8I	GCNNGC	7 cut(s)	100, 351, 612, 751, 1485, 1936, 2598
CaiI	CAGNNNCTG	2 cut(s)	1724, 2071
CfoI	GCGC	5 cut(s)	78, 93, 174, 349, 2596
Cfr13I	GGNCC	4 cut(s)	271, 310, 361, 2572
Cfr9I	CCCGGG	1 cut(s)	2265
ClaI	ATCGAT	2 cut(s)	1107, 1422
CseI	GACGC	1 cut(s)	1253
Csp6I	GTAC	5 cut(s)	582, 988, 1098, 1363, 2388
CviAII	CATG	7 cut(s)	1385, 1484, 1883, 2137, 2279, 2283, 2425
CviQI	GTAC	5 cut(s)	582, 988, 1098, 1363, 2388
DpnI	GATC	7 cut(s)	252, 396, 873, 972, 1421, 1425, 1708
DpnII	GATC	7 cut(s)	250, 394, 871, 970, 1419, 1423, 1706
DraI	TTTAAA	2 cut(s)	1929, 2314
EaeI	YGGCCR	2 cut(s)	1173, 2487
Eam1104I	CTCTTC	1 cut(s)	2104
EarI	CTCTTC	1 cut(s)	2104
EciI	GGCGGA	1 cut(s)	166
Eco105I	TACGTA	1 cut(s)	669
Eco130I	CCWWGG	2 cut(s)	182, 1587
Eco24I	GRGCYC	2 cut(s)	335, 2483
Eco47I	GGWCC	1 cut(s)	2572
Eco47III	AGCGCT	1 cut(s)	2595
Eco57I	CTGAAG	1 cut(s)	1799
Eco88I	CYCGRG	2 cut(s)	268, 2265
EcoO109I	RGGNCCY	1 cut(s)	361
EcoRII	CCWGG	4 cut(s)	306, 1681, 2254, 2495
EcoT14I	CCWWGG	2 cut(s)	182, 1587
EcoT22I	ATGCAT	2 cut(s)	1884, 2284
EcoT38I	GRGCYC	2 cut(s)	335, 2483
ErhI	CCWWGG	2 cut(s)	182, 1587
Esp3I	CGTCTC	1 cut(s)	298
FaeI	CATG	7 cut(s)	1388, 1487, 1886, 2140, 2282, 2286, 2428
FalI	AAGNNNNNCTT	2 cut(s)	2441, 2473
FaqI	GGGAC	1 cut(s)	2527
FatI	CATG	7 cut(s)	1384, 1483, 1882, 2136, 2278, 2282, 2424
FauI	CCCGC	4 cut(s)	107, 205, 344, 742
FbaI	TGATCA	1 cut(s)	970
FokI	GGATG	5 cut(s)	205, 1016, 1304, 1685, 2367
FriOI	GRGCYC	2 cut(s)	335, 2483
FspBI	CTAG	5 cut(s)	432, 567, 1889, 2421, 2597
GlaI	GCGC	5 cut(s)	77, 92, 173, 348, 2595
GsuI	CTGGAG	2 cut(s)	1665, 2540
HaeII	RGCGCY	3 cut(s)	79, 350, 2597
HaeIII	GGCC	7 cut(s)	187, 273, 312, 362, 1175, 2319, 2489
HapII	CCGG	4 cut(s)	1704, 1987, 2041, 2266
HgaI	GACGC	1 cut(s)	1253
HhaI	GCGC	5 cut(s)	78, 93, 174, 349, 2596
Hin1I	GRCGYC	1 cut(s)	1245
Hin1II	CATG	7 cut(s)	1388, 1487, 1886, 2140, 2282, 2286, 2428
Hin6I	GCGC	5 cut(s)	76, 91, 172, 347, 2594
HinP1I	GCGC	5 cut(s)	76, 91, 172, 347, 2594
HindIII	AAGCTT	1 cut(s)	1226
HinfI	GANTC	8 cut(s)	288, 734, 1556, 1597, 1866, 2096, 2225, 2507
HpaII	CCGG	4 cut(s)	1704, 1987, 2041, 2266
HphI	GGTGA	5 cut(s)	250, 500, 538, 1383, 2393
Hpy166II	GTNNAC	3 cut(s)	1520, 2432, 2564
Hpy188III	TCNNGA	6 cut(s)	404, 731, 974, 1001, 1184, 2537
Hpy8I	GTNNAC	3 cut(s)	1520, 2432, 2564
Hpy99I	CGWCG	2 cut(s)	2400, 2479
HpyCH4III	ACNGT	6 cut(s)	23, 328, 386, 661, 1720, 2387
HpyCH4IV	ACGT	3 cut(s)	458, 668, 986
HpyF10VI	GCNNNNNNNGC	8 cut(s)	99, 359, 472, 1673, 1808, 1817, 2267, 2408
HpySE526I	ACGT	3 cut(s)	458, 668, 986
Hsp92I	GRCGYC	1 cut(s)	1245
Hsp92II	CATG	7 cut(s)	1388, 1487, 1886, 2140, 2282, 2286, 2428
HspAI	GCGC	5 cut(s)	76, 91, 172, 347, 2594
Ksp22I	TGATCA	1 cut(s)	970
Kzo9I	GATC	7 cut(s)	250, 394, 871, 970, 1419, 1423, 1706
LmnI	GCTCC	4 cut(s)	179, 284, 338, 1684
Lsp1109I	GCAGC	7 cut(s)	941, 1663, 1664, 1811, 1950, 2248, 2479
MaeI	CTAG	5 cut(s)	432, 567, 1889, 2421, 2597
MaeII	ACGT	3 cut(s)	458, 668, 986
MaeIII	GTNAC	7 cut(s)	256, 769, 1087, 1612, 1909, 2381, 2453
MalI	GATC	7 cut(s)	252, 396, 873, 972, 1421, 1425, 1708
MbiI	CCGCTC	1 cut(s)	279
MboI	GATC	7 cut(s)	250, 394, 871, 970, 1419, 1423, 1706
MboII	GAAGA	8 cut(s)	667, 1148, 1391, 1701, 1792, 1888, 2091, 2525
MflI	RGATCY	1 cut(s)	1706
MhlI	GDGCHC	6 cut(s)	335, 2170, 2434, 2483, 2529, 2566
MlsI	TGGCCA	1 cut(s)	1175
MluNI	TGGCCA	1 cut(s)	1175
MlyI	GAGTC	2 cut(s)	743, 2090
MmeI	TCCRAC	2 cut(s)	476, 1032
Mox20I	TGGCCA	1 cut(s)	1175
Mph1103I	ATGCAT	2 cut(s)	1884, 2284
MroXI	GAANNNNTTC	1 cut(s)	1596
MscI	TGGCCA	1 cut(s)	1175
MslI	CAYNNNNRTG	1 cut(s)	2306
Msp20I	TGGCCA	1 cut(s)	1175
MspA1I	CMGCKG	2 cut(s)	1802, 2492
MspI	CCGG	4 cut(s)	1704, 1987, 2041, 2266
MspR9I	CCNGG	7 cut(s)	308, 1683, 2042, 2256, 2266, 2267, 2497
Mva1269I	GAATGC	3 cut(s)	1857, 2114, 2343
MvaI	CCWGG	4 cut(s)	308, 1683, 2256, 2497
MvnI	CGCG	1 cut(s)	172
MwoI	GCNNNNNNNGC	8 cut(s)	99, 359, 472, 1673, 1808, 1817, 2267, 2408
NciI	CCSGG	3 cut(s)	2042, 2266, 2267
NdeII	GATC	7 cut(s)	250, 394, 871, 970, 1419, 1423, 1706
NheI	GCTAGC	1 cut(s)	2596
NlaIII	CATG	7 cut(s)	1388, 1487, 1886, 2140, 2282, 2286, 2428
NlaIV	GGNNCC	1 cut(s)	334
NmeAIII	GCCGAG	1 cut(s)	323
NmuCI	GTSAC	4 cut(s)	256, 1612, 1909, 2381
NsiI	ATGCAT	2 cut(s)	1884, 2284
NspI	RCATGY	3 cut(s)	1388, 1487, 2140
OliI	CACNNNNGTG	1 cut(s)	2306
PaeI	GCATGC	1 cut(s)	1487
PciI	ACATGT	1 cut(s)	1384
PcsI	WCGNNNNNNNCGW	3 cut(s)	657, 674, 2535
PctI	GAATGC	3 cut(s)	1857, 2114, 2343
PdmI	GAANNNNTTC	1 cut(s)	1596
PfeI	GAWTC	6 cut(s)	288, 1556, 1597, 1866, 2225, 2507
PflMI	CCANNNNNTGG	1 cut(s)	114
PfoI	TCCNGGA	1 cut(s)	1681
PleI	GAGTC	2 cut(s)	742, 2090
PpsI	GAGTC	2 cut(s)	742, 2090
Ppu21I	YACGTR	2 cut(s)	669, 987
PscI	ACATGT	1 cut(s)	1384
PsiI	TTATAA	3 cut(s)	885, 1305, 1620
Psp6I	CCWGG	4 cut(s)	306, 1681, 2254, 2495
PspGI	CCWGG	4 cut(s)	306, 1681, 2254, 2495
PspN4I	GGNNCC	1 cut(s)	334
PspPI	GGNCC	4 cut(s)	271, 310, 361, 2572
PstNI	CAGNNNCTG	2 cut(s)	1724, 2071
PsuI	RGATCY	1 cut(s)	1706
PvuII	CAGCTG	1 cut(s)	1802
RsaI	GTAC	5 cut(s)	583, 989, 1099, 1364, 2389
RsaNI	GTAC	5 cut(s)	582, 988, 1098, 1363, 2388
RseI	CAYNNNNRTG	1 cut(s)	2306
Sau3AI	GATC	7 cut(s)	250, 394, 871, 970, 1419, 1423, 1706
Sau96I	GGNCC	4 cut(s)	271, 310, 361, 2572
ScaI	AGTACT	2 cut(s)	583, 1099
SchI	GAGTC	2 cut(s)	743, 2090
ScrFI	CCNGG	7 cut(s)	308, 1683, 2042, 2256, 2266, 2267, 2497
SduI	GDGCHC	6 cut(s)	335, 2170, 2434, 2483, 2529, 2566
SfcI	CTRYAG	4 cut(s)	1656, 1697, 1716, 2304
SinI	GGWCC	1 cut(s)	2572
SmaI	CCCGGG	1 cut(s)	2267
SmiMI	CAYNNNNRTG	1 cut(s)	2306
SmlI	CTYRAG	1 cut(s)	731
SmoI	CTYRAG	1 cut(s)	731
SnaBI	TACGTA	1 cut(s)	669
SphI	GCATGC	1 cut(s)	1487
SrfI	GCCCGGGC	1 cut(s)	2267
SspI	AATATT	1 cut(s)	1072
SspMI	CTAG	5 cut(s)	432, 567, 1889, 2421, 2597
StyD4I	CCNGG	7 cut(s)	306, 1681, 2040, 2254, 2264, 2265, 2495
StyI	CCWWGG	2 cut(s)	182, 1587
TaaI	ACNGT	6 cut(s)	23, 328, 386, 661, 1720, 2387
TaiI	ACGT	3 cut(s)	461, 671, 989
TaqII	GACCGA	2 cut(s)	1635, 2147
TatI	WGTACW	3 cut(s)	581, 1097, 1362
TauI	GCSGC	8 cut(s)	16, 19, 190, 193, 276, 279, 315, 2492
TfiI	GAWTC	6 cut(s)	288, 1556, 1597, 1866, 2225, 2507
TscAI	CASTG	3 cut(s)	26, 1674, 2523
TseFI	GTSAC	4 cut(s)	256, 1612, 1909, 2381
TseI	GCWGC	7 cut(s)	929, 1652, 1676, 1799, 1938, 2261, 2492
Tsp45I	GTSAC	4 cut(s)	256, 1612, 1909, 2381
TspDTI	ATGAA	9 cut(s)	207, 755, 1173, 1178, 1362, 1701, 1941, 2094, 2267
TspGWI	ACGGA	1 cut(s)	2037
TspMI	CCCGGG	1 cut(s)	2265
TspRI	CASTG	3 cut(s)	26, 1674, 2523
Van91I	CCANNNNNTGG	1 cut(s)	114
VneI	GTGCAC	2 cut(s)	2430, 2562
VpaK11BI	GGWCC	1 cut(s)	2572
XapI	RAATTY	3 cut(s)	763, 815, 1743
XceI	RCATGY	3 cut(s)	1388, 1487, 2140
XcmI	CCANNNNNNNNNTGG	3 cut(s)	304, 325, 2455
XmaI	CCCGGG	1 cut(s)	2265
XmnI	GAANNNNTTC	1 cut(s)	1596
XspI	CTAG	5 cut(s)	432, 567, 1889, 2421, 2597
ZrmI	AGTACT	2 cut(s)	583, 1099
Zsp2I	ATGCAT	2 cut(s)	1884, 2284

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.