FvH4_2g00810

Protein of unknown function (DUF1517)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0014649
Physical Location & Seq
Reverse (-)
831861 .. 834247
2387 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g00810.t2

Sequence Viewer

Length: 1320 bp
ATGGCGACGGCGGCTGCTAATTTGCTCTTCCAAACAAACCCGTTCAAGTGGAAGCCGAATCGACTCCGCCCCCTTGGTCCTCTTCAACCAGTCAAACTCATCATCAGTAGTGCTATCGCAACCCCGAAATCTCGTTTTTCTCTGCAGTACCATCCTCCTCCTACAGCCAAAAAGCTTCAATCATCAAATTCATCCACCTCCGATCCGAAATCAAACTCTGTATGGAAATTTGCTGTAAAAGTGACTGCGGGTGTGGTTTTGGGTGCGTTGCTGCTCATGCACAGACCCAAAACTCTGGGGCGGGTCGTTGCAACATGCAATCCCCTCGATCGCGCTCAAAAGGATGATCCTGAGTGTGCTGATCGGTACAGGTCAGCGCAGTGGTACAATTTGTTGCGTACCTTTTGCGAGACTGCAGGGTTAAGTTTATATCCTTGTGCAGCATACTATGGCTCGCTTCCTGTGCTCTACATATTGTCTGAATATCTAACACTGACCGACCCTTCTAATAAGCTCCATTGGTCTAAACACTGGTACGCTTGGTATAAGCGCCAAGCCAACTTCTTGTTGATATTCCTTCTCATAGCTCATATTACTTGGGCATGGCGCCATATTCCATCTACTGTTGTCAGGCTTAACATCCAGGTTAATTTATCGAATGCTGGACGAGCGCTCCACACTGATCTTAATCGGATTGCTGAAACTACTGCAGATACCTCTATCTATAAAGGTGTAAACAGTATTTTGACAGAGACAGCAACAACTTTTCTTAAGCATCCTGATTGTTTCATCTCAGCCAGTACGTATGCGGCTGGCCAGTCTCATTGGGATGATGATGCACGAAATTTCTTAAATGAACTATGTCTTGAAGAGAGGATCAGATTAGGCGGACATCCACTTATCAATGTGAATAAGAACTTAAAAGTGGAAAGCTCAACAAGCCAAAAATCAGCACATGAAATTCAACATGAACATATTGACGTAGATGCTAATCAACTTGACAAACACAACAAATATGACTTGTCCCACCTAGTTCATACCCCGCTCATGGCGTCAAAGAAGATAGATCTTACGCAGGAAAGCTCAACAAGCCAAATATCGGCAACTGAAACGCAAGATGAATATATGGAGGTTATAATATTGGCGGCTATTGCCTCGAAGCGTGATGATGAGTTTCCCAGAATCATCAAATCCTGGGATGACTTGAAGGAAGCATTGCAATTGCTTACATCCGAGGGTATATATCCATATGTAGTAGAAGTGTTGTGGAAGCCTATGTCCTCTTCCTCGACGGGCAGGAGAAGATTGTGGTTCAGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

440

Amino Acids

49.76

Weight (kDa)

9.09

Isoelectric Point (pI)

43.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1517 PF07466 212 - 318 6.1e-13 Protein of unknown function (DUF1517)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1136
AccB1I GGYRCC 1 cut(s) 606
AccBSI CCGCTC 1 cut(s) 1045
AccII CGCG 1 cut(s) 333
AciI CCGC 8 cut(s) 11, 67, 248, 301, 809, 888, 1043, 1145
AclWI GGATC 3 cut(s) 197, 341, 884
AcoI YGGCCR 1 cut(s) 814
AcsI RAATTY 4 cut(s) 187, 227, 844, 960
AcyI GRCGYC 2 cut(s) 607, 1052
AfaI GTAC 6 cut(s) 149, 368, 386, 400, 536, 802
AfeI AGCGCT 1 cut(s) 672
AfiI CCNNNNNNNGG 2 cut(s) 1048, 1099
AflII CTTAAG 1 cut(s) 770
AgsI TTSAA 6 cut(s) 46, 86, 179, 869, 965, 1207
AjnI CCWGG 2 cut(s) 642, 1193
AluBI AGCT 5 cut(s) 175, 514, 587, 933, 1083
AluI AGCT 5 cut(s) 175, 514, 587, 933, 1083
Alw21I GWGCWC 1 cut(s) 468
Alw26I GTCTC 3 cut(s) 404, 746, 825
AlwI GGATC 3 cut(s) 197, 341, 884
Aor51HI AGCGCT 1 cut(s) 672
AoxI GGCC 1 cut(s) 814
ApeKI GCWGC 3 cut(s) 14, 271, 440
ApoI RAATTY 4 cut(s) 187, 227, 844, 960
AspLEI GCGC 5 cut(s) 335, 379, 552, 609, 673
AspS9I GGNCC 1 cut(s) 77
AvaII GGWCC 1 cut(s) 77
BalI TGGCCA 1 cut(s) 816
BanI GGYRCC 1 cut(s) 606
Bbv12I GWGCWC 1 cut(s) 468
BbvI GCAGC 2 cut(s) 258, 452
BccI CCATC 2 cut(s) 159, 625
BceAI ACGGC 1 cut(s) 24
BcgI CGANNNNNNTGC 2 cut(s) 307, 341
BciT130I CCWGG 2 cut(s) 644, 1195
BcoDI GTCTC 3 cut(s) 404, 746, 825
BfaI CTAG 1 cut(s) 1031
BfmI CTRYAG 4 cut(s) 143, 162, 414, 708
BfoI RGCGCY 3 cut(s) 553, 610, 674
BfrI CTTAAG 1 cut(s) 770
BglII AGATCT 1 cut(s) 1066
BisI GCNGC 6 cut(s) 12, 15, 272, 441, 810, 1146
BlsI GCNGC 6 cut(s) 13, 16, 273, 442, 811, 1147
Bme1390I CCNGG 2 cut(s) 644, 1195
Bme18I GGWCC 1 cut(s) 77
BmgT120I GGNCC 1 cut(s) 77
BmiI GGNNCC 1 cut(s) 608
BmrFI CCNGG 2 cut(s) 644, 1195
BmsI GCATC 3 cut(s) 784, 826, 976
BsaAI YACGTR 1 cut(s) 804
BsaBI GATNNNNATC 2 cut(s) 687, 990
BsaHI GRCGYC 2 cut(s) 607, 1052
BsaJI CCNNGG 3 cut(s) 73, 1194, 1233
BsaXI ACNNNNNCTCC 2 cut(s) 657, 687
Bsc4I CCNNNNNNNGG 2 cut(s) 1048, 1099
Bse1I ACTGG 4 cut(s) 89, 536, 798, 817
Bse3DI GCAATG 1 cut(s) 1214
Bse8I GATNNNNATC 2 cut(s) 687, 990
BseBI CCWGG 2 cut(s) 644, 1195
BseDI CCNNGG 3 cut(s) 73, 1194, 1233
BseGI GGATG 9 cut(s) 151, 191, 349, 639, 775, 835, 892, 1204, 1229
BseJI GATNNNNATC 2 cut(s) 687, 990
BseLI CCNNNNNNNGG 2 cut(s) 1048, 1099
BseMI GCAATG 1 cut(s) 1214
BseMII CTCAG 2 cut(s) 342, 807
BseNI ACTGG 4 cut(s) 89, 536, 798, 817
BseRI GAGGAG 1 cut(s) 147
BseXI GCAGC 2 cut(s) 258, 452
BsgI GTGCAG 1 cut(s) 459
Bsh1236I CGCG 1 cut(s) 333
Bsh1285I CGRYCG 1 cut(s) 331
BshFI GGCC 1 cut(s) 816
BshNI GGYRCC 1 cut(s) 606
BsiEI CGRYCG 1 cut(s) 331
BsiHKAI GWGCWC 1 cut(s) 468
BslFI GGGAC 1 cut(s) 1009
BslI CCNNNNNNNGG 2 cut(s) 1048, 1099
BsmAI GTCTC 3 cut(s) 404, 746, 825
BsmFI GGGAC 1 cut(s) 1009
BsmI GAATGC 1 cut(s) 664
BsnI GGCC 1 cut(s) 816
Bsp1286I GDGCHC 1 cut(s) 468
Bsp143I GATC 7 cut(s) 202, 328, 346, 361, 682, 876, 1066
BspACI CCGC 8 cut(s) 11, 67, 248, 301, 809, 888, 1043, 1145
BspANI GGCC 1 cut(s) 816
BspCNI CTCAG 2 cut(s) 343, 806
BspFNI CGCG 1 cut(s) 333
BspLI GGNNCC 1 cut(s) 608
BspMAI CTGCAG 3 cut(s) 147, 418, 712
BspPI GGATC 3 cut(s) 197, 341, 884
BspQI GCTCTTC 1 cut(s) 32
BspT107I GGYRCC 1 cut(s) 606
BspTI CTTAAG 1 cut(s) 770
BsrBI CCGCTC 1 cut(s) 1045
BsrDI GCAATG 1 cut(s) 1214
BsrI ACTGG 4 cut(s) 89, 536, 798, 817
BssECI CCNNGG 3 cut(s) 73, 1194, 1233
BssMI GATC 7 cut(s) 202, 328, 346, 361, 682, 876, 1066
BssNI GRCGYC 2 cut(s) 607, 1052
BssT1I CCWWGG 1 cut(s) 73
Bst2UI CCWGG 2 cut(s) 644, 1195
Bst4CI ACNGT 2 cut(s) 625, 740
Bst6I CTCTTC 4 cut(s) 32, 87, 864, 1288
BstACI GRCGYC 2 cut(s) 607, 1052
BstAFI CTTAAG 1 cut(s) 770
BstBAI YACGTR 1 cut(s) 804
BstC8I GCNNGC 2 cut(s) 455, 814
BstDEI CTNAG 2 cut(s) 351, 793
BstF5I GGATG 9 cut(s) 151, 191, 349, 639, 775, 835, 892, 1204, 1229
BstFNI CGCG 1 cut(s) 333
BstH2I RGCGCY 3 cut(s) 553, 610, 674
BstHHI GCGC 5 cut(s) 335, 379, 552, 609, 673
BstKTI GATC 7 cut(s) 205, 331, 349, 364, 685, 879, 1069
BstMAI GTCTC 3 cut(s) 404, 746, 825
BstMBI GATC 7 cut(s) 202, 328, 346, 361, 682, 876, 1066
BstMCI CGRYCG 1 cut(s) 331
BstMWI GCNNNNNNNGC 7 cut(s) 11, 277, 463, 668, 939, 1089, 1151
BstNI CCWGG 2 cut(s) 644, 1195
BstNSI RCATGY 1 cut(s) 318
BstSCI CCNGG 2 cut(s) 642, 1193
BstSFI CTRYAG 4 cut(s) 143, 162, 414, 708
BstSNI TACGTA 1 cut(s) 804
BstUI CGCG 1 cut(s) 333
BstV1I GCAGC 2 cut(s) 258, 452
BstX2I RGATCY 1 cut(s) 1066
BstXI CCANNNNNNTGG 1 cut(s) 295
BstYI RGATCY 1 cut(s) 1066
BsuRI GGCC 1 cut(s) 816
BtsCI GGATG 9 cut(s) 151, 191, 349, 639, 775, 835, 892, 1204, 1229
BtsI GCAGTG 1 cut(s) 386
BtsIMutI CAGTG 4 cut(s) 386, 491, 529, 678
Cac8I GCNNGC 2 cut(s) 455, 814
CfoI GCGC 5 cut(s) 335, 379, 552, 609, 673
Cfr13I GGNCC 1 cut(s) 77
CseI GACGC 1 cut(s) 1041
Csp6I GTAC 6 cut(s) 148, 367, 385, 399, 535, 801
CviAII CATG 6 cut(s) 277, 315, 603, 956, 968, 1048
CviQI GTAC 6 cut(s) 148, 367, 385, 399, 535, 801
DdeI CTNAG 2 cut(s) 351, 793
DinI GGCGCC 1 cut(s) 608
DpnI GATC 7 cut(s) 204, 330, 348, 363, 684, 878, 1068
DpnII GATC 7 cut(s) 202, 328, 346, 361, 682, 876, 1066
EaeI YGGCCR 1 cut(s) 814
Eam1104I CTCTTC 4 cut(s) 32, 87, 864, 1288
EarI CTCTTC 4 cut(s) 32, 87, 864, 1288
EciI GGCGGA 2 cut(s) 56, 903
Eco105I TACGTA 1 cut(s) 804
Eco130I CCWWGG 1 cut(s) 73
Eco47I GGWCC 1 cut(s) 77
Eco47III AGCGCT 1 cut(s) 672
EcoRII CCWGG 2 cut(s) 642, 1193
EcoT14I CCWWGG 1 cut(s) 73
EgeI GGCGCC 1 cut(s) 608
EheI GGCGCC 1 cut(s) 608
ErhI CCWWGG 1 cut(s) 73
FaeI CATG 6 cut(s) 280, 318, 606, 959, 971, 1051
FaqI GGGAC 1 cut(s) 1009
FatI CATG 6 cut(s) 276, 314, 602, 955, 967, 1047
FauI CCCGC 3 cut(s) 241, 294, 1050
FauNDI CATATG 1 cut(s) 1249
Fnu4HI GCNGC 6 cut(s) 12, 15, 272, 441, 810, 1146
FokI GGATG 9 cut(s) 138, 178, 356, 626, 762, 842, 879, 1211, 1216
Fsp4HI GCNGC 6 cut(s) 12, 15, 272, 441, 810, 1146
FspBI CTAG 1 cut(s) 1031
GlaI GCGC 5 cut(s) 334, 378, 551, 608, 672
GluI GCNGC 6 cut(s) 12, 15, 272, 441, 810, 1146
HaeII RGCGCY 3 cut(s) 553, 610, 674
HaeIII GGCC 1 cut(s) 816
HgaI GACGC 1 cut(s) 1041
HhaI GCGC 5 cut(s) 335, 379, 552, 609, 673
Hin1I GRCGYC 2 cut(s) 607, 1052
Hin1II CATG 6 cut(s) 280, 318, 606, 959, 971, 1051
Hin6I GCGC 5 cut(s) 333, 377, 550, 607, 671
HinP1I GCGC 5 cut(s) 333, 377, 550, 607, 671
HindIII AAGCTT 1 cut(s) 173
HinfI GANTC 3 cut(s) 58, 63, 1182
Hpy166II GTNNAC 1 cut(s) 736
Hpy188I TCNGA 7 cut(s) 202, 207, 481, 693, 881, 1234, 1316
Hpy188III TCNNGA 3 cut(s) 350, 779, 866
Hpy8I GTNNAC 1 cut(s) 736
Hpy99I CGWCG 2 cut(s) 10, 1294
HpyAV CCTTC 3 cut(s) 513, 587, 1201
HpyCH4III ACNGT 2 cut(s) 625, 740
HpyCH4IV ACGT 2 cut(s) 803, 981
HpyCH4V TGCA 9 cut(s) 145, 280, 311, 318, 416, 440, 710, 839, 1219
HpyF10VI GCNNNNNNNGC 7 cut(s) 11, 277, 463, 668, 939, 1089, 1151
HpyF3I CTNAG 2 cut(s) 351, 793
HpySE526I ACGT 2 cut(s) 803, 981
Hsp92I GRCGYC 2 cut(s) 607, 1052
Hsp92II CATG 6 cut(s) 280, 318, 606, 959, 971, 1051
HspAI GCGC 5 cut(s) 333, 377, 550, 607, 671
KasI GGCGCC 1 cut(s) 606
Kzo9I GATC 7 cut(s) 202, 328, 346, 361, 682, 876, 1066
LguI GCTCTTC 1 cut(s) 32
LmnI GCTCC 2 cut(s) 519, 678
Lsp1109I GCAGC 2 cut(s) 258, 452
LweI GCATC 3 cut(s) 784, 826, 976
MaeI CTAG 1 cut(s) 1031
MaeII ACGT 2 cut(s) 803, 981
MaeIII GTNAC 1 cut(s) 241
MalI GATC 7 cut(s) 204, 330, 348, 363, 684, 878, 1068
MbiI CCGCTC 1 cut(s) 1045
MboI GATC 7 cut(s) 202, 328, 346, 361, 682, 876, 1066
MboII GAAGA 6 cut(s) 19, 74, 881, 1072, 1275, 1314
MfeI CAATTG 1 cut(s) 1220
MflI RGATCY 1 cut(s) 1066
MhlI GDGCHC 1 cut(s) 468
MlsI TGGCCA 1 cut(s) 816
MluCI AATT 8 cut(s) 19, 187, 227, 388, 649, 844, 960, 1220
MluNI TGGCCA 1 cut(s) 816
Mly113I GGCGCC 1 cut(s) 607
MlyI GAGTC 1 cut(s) 57
Mox20I TGGCCA 1 cut(s) 816
MscI TGGCCA 1 cut(s) 816
MseI TTAA 7 cut(s) 422, 636, 648, 687, 771, 851, 920
MslI CAYNNNNRTG 1 cut(s) 828
Msp20I TGGCCA 1 cut(s) 816
MspCI CTTAAG 1 cut(s) 770
MspR9I CCNGG 2 cut(s) 644, 1195
MunI CAATTG 1 cut(s) 1220
Mva1269I GAATGC 1 cut(s) 664
MvaI CCWGG 2 cut(s) 644, 1195
MvnI CGCG 1 cut(s) 333
MwoI GCNNNNNNNGC 7 cut(s) 11, 277, 463, 668, 939, 1089, 1151
NarI GGCGCC 1 cut(s) 607
NdeI CATATG 1 cut(s) 1249
NdeII GATC 7 cut(s) 202, 328, 346, 361, 682, 876, 1066
NlaIII CATG 6 cut(s) 280, 318, 606, 959, 971, 1051
NlaIV GGNNCC 1 cut(s) 608
NmuCI GTSAC 1 cut(s) 241
NspI RCATGY 1 cut(s) 318
PciSI GCTCTTC 1 cut(s) 32
PctI GAATGC 1 cut(s) 664
PfeI GAWTC 2 cut(s) 58, 1182
PkrI GCNGC 6 cut(s) 13, 16, 273, 442, 811, 1147
Ple19I CGATCG 1 cut(s) 331
PleI GAGTC 1 cut(s) 57
PluTI GGCGCC 1 cut(s) 610
PpsI GAGTC 1 cut(s) 57
Ppu21I YACGTR 1 cut(s) 804
PsiI TTATAA 1 cut(s) 1136
Psp6I CCWGG 2 cut(s) 642, 1193
PspGI CCWGG 2 cut(s) 642, 1193
PspN4I GGNNCC 1 cut(s) 608
PspPI GGNCC 1 cut(s) 77
PstI CTGCAG 3 cut(s) 147, 418, 712
PsuI RGATCY 1 cut(s) 1066
PvuI CGATCG 1 cut(s) 331
RsaI GTAC 6 cut(s) 149, 368, 386, 400, 536, 802
RsaNI GTAC 6 cut(s) 148, 367, 385, 399, 535, 801
RseI CAYNNNNRTG 1 cut(s) 828
SapI GCTCTTC 1 cut(s) 32
SaqAI TTAA 7 cut(s) 422, 636, 648, 687, 771, 851, 920
SatI GCNGC 6 cut(s) 12, 15, 272, 441, 810, 1146
Sau3AI GATC 7 cut(s) 202, 328, 346, 361, 682, 876, 1066
Sau96I GGNCC 1 cut(s) 77
SchI GAGTC 1 cut(s) 57
ScrFI CCNGG 2 cut(s) 644, 1195
SduI GDGCHC 1 cut(s) 468
SfaNI GCATC 3 cut(s) 784, 826, 976
SfcI CTRYAG 4 cut(s) 143, 162, 414, 708
SfoI GGCGCC 1 cut(s) 608
SinI GGWCC 1 cut(s) 77
SmiMI CAYNNNNRTG 1 cut(s) 828
SmlI CTYRAG 1 cut(s) 770
SmoI CTYRAG 1 cut(s) 770
SnaBI TACGTA 1 cut(s) 804
Sse9I AATT 8 cut(s) 19, 187, 227, 388, 649, 844, 960, 1220
SsiI CCGC 8 cut(s) 11, 67, 248, 301, 809, 888, 1043, 1145
SspDI GGCGCC 1 cut(s) 606
SspI AATATT 1 cut(s) 1140
SspMI CTAG 1 cut(s) 1031
StyD4I CCNGG 2 cut(s) 642, 1193
StyI CCWWGG 1 cut(s) 73
TaaI ACNGT 2 cut(s) 625, 740
TaiI ACGT 2 cut(s) 806, 984
TaqI TCGA 5 cut(s) 61, 327, 656, 1157, 1289
TaqII GACCGA 1 cut(s) 512
TasI AATT 8 cut(s) 19, 187, 227, 388, 649, 844, 960, 1220
TauI GCSGC 3 cut(s) 14, 812, 1148
TfiI GAWTC 2 cut(s) 58, 1182
Tru1I TTAA 7 cut(s) 422, 636, 648, 687, 771, 851, 920
Tru9I TTAA 7 cut(s) 422, 636, 648, 687, 771, 851, 920
TscAI CASTG 4 cut(s) 386, 498, 536, 685
TseFI GTSAC 1 cut(s) 241
TseI GCWGC 3 cut(s) 14, 271, 440
Tsp45I GTSAC 1 cut(s) 241
TspDTI ATGAA 7 cut(s) 180, 778, 870, 972, 984, 1025, 1134
TspRI CASTG 4 cut(s) 386, 498, 536, 685
Vha464I CTTAAG 1 cut(s) 770
VpaK11BI GGWCC 1 cut(s) 77
XapI RAATTY 4 cut(s) 187, 227, 844, 960
XceI RCATGY 1 cut(s) 318
XspI CTAG 1 cut(s) 1031
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.