FvH4_5g04910

Protein of unknown function (DUF1517)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0014649
Physical Location & Seq
Forward (+)
2922872 .. 2925804
2933 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g04910.t1

Sequence Viewer

Length: 1158 bp
ATGGCAACCGCGACAGCTAATCTGCTCATCCAAACAAACACATGGAAGAGGAAGCAAAGCTACCCTAATCTACTCCGCCCTCTTCCGGCGGCCCGACTAGTTAACCTCAGGCCAACTTTCAACCAACTTAAACTCAAAGCCATCGTCTCGCCGGCGGCGGCACAGAAGAAGAGCCATTTAATCACCAATCCAACCCAATCTCTGTTGCAACCTACTACTGTTGAAAAAATCCGGCCACCCAATAAACTATGGAAGGCACTGAAGAAACCCGTGACGGCGGTCGTGCTTTTGGGTCTATTACTGATGCACAGACCCAAACCGGCACTGGCCGGCTCCGTCGGGCGGATCGGTGCAACATGCTCGCCCTGGGATTCTCCTCCAAAATCCGAATCGCGGAAGCGGTTCGAAGAAGCGATGGAGGTCAACATGGAGGAGAGGTCTTTTGCAACTGTGTGGATTATCATGTATCCTATTGCGGCATACTATTGCGGACTCGCTGTGCTATTTGTATTGTGCCGATTGCTAAAATTGGTCGACCCTTCTTGGCTCGACTCCGCTAATGACCGAACTAAGTACTTGTTAGTGCTGCTAATTCTTCACTATGCTTGGGTCACTACCTATGATCCAGTAACCGTTTTCAAGCTTAACCTGCAGGCTAATTTGTCGAACACCGGGAGAGCACTCCAAACAGATCTTAATCGAATAGCTGAAAATCCAAATACTTCTACCTATGAGGGTTTAAACAGTATCTTGACAGAGACAGCAGTAGCTTTTCTTAGGCATTCTGATTGTTTCATCTCCGCCAGCTCATTTGTGGCTGAGAAATACGGAATGAAGCGTGCACAAAAGTACTTAAGTCAACTATCTCTCGAAGAGACGAATAAATTACGCGAAAAACCGTTTGTCAATGTGAAGAACTTAATAGCGGAAAGCTCAACGAGCCAAAAATCAGCTAATGAAGTCCAAGAGGAATATATGGAGGTTACGATATTGGTGGCTGTTGAGGGGAGGCACAAGTTGCCCACCATTAATTCCGTGGATGACCTGAAGAAAGTATTGCGAAAACTCGCATCCATTGATCCTGATGGCAACATATACGTATATGGAGTAGAAGTGTTGTGGCATCCTCAATCCTCTCTGAGCAGGACAAGATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

386

Amino Acids

43.29

Weight (kDa)

9.65

Isoelectric Point (pI)

48.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1517 PF07466 216 - 378 2e-35 Protein of unknown function (DUF1517)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 657
AccI GTMKAC 1 cut(s) 534
AccII CGCG 3 cut(s) 11, 394, 891
AclWI GGATC 3 cut(s) 353, 617, 1073
AcoI YGGCCR 2 cut(s) 233, 327
AcuI CTGAAG 2 cut(s) 281, 1067
AfaI GTAC 2 cut(s) 575, 851
AfiI CCNNNNNNNGG 3 cut(s) 85, 342, 393
AflII CTTAAG 1 cut(s) 853
AgsI TTSAA 3 cut(s) 121, 224, 640
AhlI ACTAGT 1 cut(s) 97
AjnI CCWGG 1 cut(s) 365
AluBI AGCT 8 cut(s) 17, 60, 643, 707, 770, 807, 933, 953
AluI AGCT 8 cut(s) 17, 60, 643, 707, 770, 807, 933, 953
Alw21I GWGCWC 2 cut(s) 682, 844
Alw26I GTCTC 3 cut(s) 151, 752, 869
Alw44I GTGCAC 1 cut(s) 840
AlwI GGATC 3 cut(s) 353, 617, 1073
AoxI GGCC 4 cut(s) 90, 110, 233, 327
ApaLI GTGCAC 1 cut(s) 840
ApeKI GCWGC 1 cut(s) 586
ArsI GACNNNNNNTTYG 2 cut(s) 129, 161
AseI ATTAAT 1 cut(s) 1029
Asp700I GAANNNNTTC 1 cut(s) 401
AspS9I GGNCC 1 cut(s) 91
AsuC2I CCSGG 1 cut(s) 673
AsuHPI GGTGA 1 cut(s) 175
AsuII TTCGAA 1 cut(s) 405
AxyI CCTNAGG 1 cut(s) 107
BaeGI GKGCMC 1 cut(s) 844
BarI GAAGNNNNNNTAC 2 cut(s) 44, 76
Bbv12I GWGCWC 2 cut(s) 682, 844
BbvI GCAGC 1 cut(s) 573
BccI CCATC 3 cut(s) 149, 409, 1079
BceAI ACGGC 1 cut(s) 291
BcgI CGANNNNNNTGC 2 cut(s) 342, 376
BciT130I CCWGG 1 cut(s) 367
BciVI GTATCC 1 cut(s) 477
BcnI CCSGG 1 cut(s) 673
BcoDI GTCTC 3 cut(s) 151, 752, 869
BcuI ACTAGT 1 cut(s) 97
BfaI CTAG 1 cut(s) 98
BfmI CTRYAG 1 cut(s) 650
BfrI CTTAAG 1 cut(s) 853
BfuAI ACCTGC 1 cut(s) 657
BfuI GTATCC 1 cut(s) 477
BglII AGATCT 1 cut(s) 691
BisI GCNGC 5 cut(s) 90, 156, 159, 477, 587
BlsI GCNGC 5 cut(s) 91, 157, 160, 478, 588
BmcAI AGTACT 2 cut(s) 575, 851
Bme1390I CCNGG 2 cut(s) 367, 673
BmgT120I GGNCC 1 cut(s) 91
BmiI GGNNCC 1 cut(s) 334
BmrFI CCNGG 2 cut(s) 367, 673
BmsI GCATC 3 cut(s) 294, 1079, 1132
BoxI GACNNNNGTC 1 cut(s) 278
Bpu14I TTCGAA 1 cut(s) 405
BpuMI CCSGG 1 cut(s) 673
BsaAI YACGTR 1 cut(s) 1099
BsaBI GATNNNNATC 1 cut(s) 696
BsaJI CCNNGG 3 cut(s) 365, 366, 1035
BsaXI ACNNNNNCTCC 4 cut(s) 422, 452, 1098, 1128
Bsc4I CCNNNNNNNGG 3 cut(s) 85, 342, 393
Bse118I RCCGGY 3 cut(s) 151, 319, 329
Bse1I ACTGG 2 cut(s) 330, 626
Bse21I CCTNAGG 1 cut(s) 107
Bse8I GATNNNNATC 1 cut(s) 696
BseBI CCWGG 1 cut(s) 367
BseDI CCNNGG 3 cut(s) 365, 366, 1035
BseGI GGATG 4 cut(s) 27, 1045, 1070, 1123
BseJI GATNNNNATC 1 cut(s) 696
BseLI CCNNNNNNNGG 3 cut(s) 85, 342, 393
BseMII CTCAG 3 cut(s) 121, 810, 1130
BseNI ACTGG 2 cut(s) 330, 626
BseRI GAGGAG 2 cut(s) 366, 446
BseSI GKGCMC 1 cut(s) 844
BseXI GCAGC 1 cut(s) 573
Bsh1236I CGCG 3 cut(s) 11, 394, 891
Bsh1285I CGRYCG 1 cut(s) 282
BshFI GGCC 4 cut(s) 92, 112, 235, 329
BsiEI CGRYCG 1 cut(s) 282
BsiHKAI GWGCWC 2 cut(s) 682, 844
BsiSI CCGG 6 cut(s) 86, 152, 232, 320, 330, 672
BslI CCNNNNNNNGG 3 cut(s) 85, 342, 393
BsmAI GTCTC 3 cut(s) 151, 752, 869
BsmBI CGTCTC 2 cut(s) 151, 869
BsmI GAATGC 1 cut(s) 781
BsnI GGCC 4 cut(s) 92, 112, 235, 329
Bsp119I TTCGAA 1 cut(s) 405
Bsp1286I GDGCHC 2 cut(s) 682, 844
Bsp143I GATC 4 cut(s) 345, 622, 691, 1078
BspANI GGCC 4 cut(s) 92, 112, 235, 329
BspCNI CTCAG 3 cut(s) 120, 811, 1131
BspFNI CGCG 3 cut(s) 11, 394, 891
BspLI GGNNCC 1 cut(s) 334
BspMAI CTGCAG 1 cut(s) 654
BspMI ACCTGC 1 cut(s) 657
BspPI GGATC 3 cut(s) 353, 617, 1073
BspQI GCTCTTC 1 cut(s) 164
BspT104I TTCGAA 1 cut(s) 405
BspTI CTTAAG 1 cut(s) 853
BsrFI RCCGGY 3 cut(s) 151, 319, 329
BsrI ACTGG 2 cut(s) 330, 626
BssAI RCCGGY 3 cut(s) 151, 319, 329
BssECI CCNNGG 3 cut(s) 365, 366, 1035
BssMI GATC 4 cut(s) 345, 622, 691, 1078
Bst2UI CCWGG 1 cut(s) 367
Bst4CI ACNGT 5 cut(s) 220, 451, 634, 746, 900
Bst6I CTCTTC 4 cut(s) 41, 87, 164, 867
BstAFI CTTAAG 1 cut(s) 853
BstAPI GCANNNNNTGC 1 cut(s) 1018
BstBAI YACGTR 1 cut(s) 1099
BstBI TTCGAA 1 cut(s) 405
BstC8I GCNNGC 6 cut(s) 153, 331, 362, 654, 805, 840
BstDEI CTNAG 5 cut(s) 107, 570, 776, 819, 1139
BstDSI CCRYGG 1 cut(s) 1035
BstF5I GGATG 4 cut(s) 27, 1045, 1070, 1123
BstFNI CGCG 3 cut(s) 11, 394, 891
BstKTI GATC 4 cut(s) 348, 625, 694, 1081
BstMAI GTCTC 3 cut(s) 151, 752, 869
BstMBI GATC 4 cut(s) 345, 622, 691, 1078
BstMCI CGRYCG 1 cut(s) 282
BstMWI GCNNNNNNNGC 3 cut(s) 649, 939, 1018
BstNI CCWGG 1 cut(s) 367
BstNSI RCATGY 1 cut(s) 360
BstPAI GACNNNNGTC 1 cut(s) 278
BstSCI CCNGG 2 cut(s) 365, 671
BstSFI CTRYAG 1 cut(s) 650
BstSLI GKGCMC 1 cut(s) 844
BstSNI TACGTA 1 cut(s) 1099
BstUI CGCG 3 cut(s) 11, 394, 891
BstV1I GCAGC 1 cut(s) 573
BstX2I RGATCY 1 cut(s) 691
BstYI RGATCY 1 cut(s) 691
Bsu36I CCTNAGG 1 cut(s) 107
BsuI GTATCC 1 cut(s) 477
BsuRI GGCC 4 cut(s) 92, 112, 235, 329
BtgI CCRYGG 1 cut(s) 1035
BtgZI GCGATG 1 cut(s) 428
BtsCI GGATG 4 cut(s) 27, 1045, 1070, 1123
BtsIMutI CAGTG 2 cut(s) 257, 323
BveI ACCTGC 1 cut(s) 657
Cac8I GCNNGC 6 cut(s) 153, 331, 362, 654, 805, 840
Cfr10I RCCGGY 3 cut(s) 151, 319, 329
Cfr13I GGNCC 1 cut(s) 91
Csp6I GTAC 2 cut(s) 574, 850
CviAII CATG 4 cut(s) 42, 357, 427, 463
CviQI GTAC 2 cut(s) 574, 850
DdeI CTNAG 5 cut(s) 107, 570, 776, 819, 1139
DpnI GATC 4 cut(s) 347, 624, 693, 1080
DpnII GATC 4 cut(s) 345, 622, 691, 1078
DraI TTTAAA 1 cut(s) 741
EaeI YGGCCR 2 cut(s) 233, 327
Eam1104I CTCTTC 4 cut(s) 41, 87, 164, 867
EarI CTCTTC 4 cut(s) 41, 87, 164, 867
EciI GGCGGA 3 cut(s) 65, 358, 790
Eco105I TACGTA 1 cut(s) 1099
Eco57I CTGAAG 2 cut(s) 281, 1067
Eco81I CCTNAGG 1 cut(s) 107
EcoRII CCWGG 1 cut(s) 365
Esp3I CGTCTC 2 cut(s) 151, 869
FaeI CATG 4 cut(s) 45, 360, 430, 466
FatI CATG 4 cut(s) 41, 356, 426, 462
FblI GTMKAC 1 cut(s) 534
Fnu4HI GCNGC 5 cut(s) 90, 156, 159, 477, 587
FokI GGATG 4 cut(s) 14, 1052, 1057, 1110
Fsp4HI GCNGC 5 cut(s) 90, 156, 159, 477, 587
FspBI CTAG 1 cut(s) 98
GluI GCNGC 5 cut(s) 90, 156, 159, 477, 587
HaeIII GGCC 4 cut(s) 92, 112, 235, 329
HapII CCGG 6 cut(s) 86, 152, 232, 320, 330, 672
Hin1II CATG 4 cut(s) 45, 360, 430, 466
HincII GTYRAC 4 cut(s) 103, 424, 535, 860
HindII GTYRAC 4 cut(s) 103, 424, 535, 860
HindIII AAGCTT 1 cut(s) 641
HinfI GANTC 4 cut(s) 371, 389, 492, 551
HpaI GTTAAC 1 cut(s) 103
HpaII CCGG 6 cut(s) 86, 152, 232, 320, 330, 672
HphI GGTGA 1 cut(s) 175
Hpy166II GTNNAC 5 cut(s) 103, 424, 535, 842, 860
Hpy188I TCNGA 3 cut(s) 388, 787, 1140
Hpy188III TCNNGA 3 cut(s) 751, 869, 1082
Hpy8I GTNNAC 5 cut(s) 103, 424, 535, 842, 860
Hpy99I CGWCG 1 cut(s) 341
HpyAV CCTTC 2 cut(s) 247, 549
HpyCH4III ACNGT 5 cut(s) 220, 451, 634, 746, 900
HpyCH4IV ACGT 1 cut(s) 1098
HpyCH4V TGCA 6 cut(s) 208, 307, 353, 446, 652, 842
HpyF10VI GCNNNNNNNGC 3 cut(s) 649, 939, 1018
HpyF3I CTNAG 5 cut(s) 107, 570, 776, 819, 1139
HpySE526I ACGT 1 cut(s) 1098
Hsp92II CATG 4 cut(s) 45, 360, 430, 466
KroI GCCGGC 2 cut(s) 151, 329
KroNI GCCGGC 2 cut(s) 153, 331
KspAI GTTAAC 1 cut(s) 103
Kzo9I GATC 4 cut(s) 345, 622, 691, 1078
LguI GCTCTTC 1 cut(s) 164
LmnI GCTCC 1 cut(s) 338
Lsp1109I GCAGC 1 cut(s) 573
LweI GCATC 3 cut(s) 294, 1079, 1132
MaeI CTAG 1 cut(s) 98
MaeII ACGT 1 cut(s) 1098
MaeIII GTNAC 4 cut(s) 271, 610, 628, 982
MalI GATC 4 cut(s) 347, 624, 693, 1080
MboI GATC 4 cut(s) 345, 622, 691, 1078
MflI RGATCY 1 cut(s) 691
MhlI GDGCHC 2 cut(s) 682, 844
MluCI AATT 5 cut(s) 527, 591, 658, 884, 1030
MlyI GAGTC 2 cut(s) 486, 545
MmeI TCCRAC 1 cut(s) 215
MreI CGCCGGCG 1 cut(s) 151
MroNI GCCGGC 2 cut(s) 151, 329
MroXI GAANNNNTTC 1 cut(s) 401
MseI TTAA 9 cut(s) 102, 129, 179, 645, 696, 740, 854, 920, 1029
MspCI CTTAAG 1 cut(s) 853
MspI CCGG 6 cut(s) 86, 152, 232, 320, 330, 672
MspR9I CCNGG 2 cut(s) 367, 673
MssI GTTTAAAC 1 cut(s) 741
Mva1269I GAATGC 1 cut(s) 781
MvaI CCWGG 1 cut(s) 367
MvnI CGCG 3 cut(s) 11, 394, 891
MwoI GCNNNNNNNGC 3 cut(s) 649, 939, 1018
NaeI GCCGGC 2 cut(s) 153, 331
NciI CCSGG 1 cut(s) 673
NdeII GATC 4 cut(s) 345, 622, 691, 1078
NgoMIV GCCGGC 2 cut(s) 151, 329
NlaIII CATG 4 cut(s) 45, 360, 430, 466
NlaIV GGNNCC 1 cut(s) 334
NmuCI GTSAC 2 cut(s) 271, 610
NspI RCATGY 1 cut(s) 360
NspV TTCGAA 1 cut(s) 405
PasI CCCWGGG 1 cut(s) 366
PciSI GCTCTTC 1 cut(s) 164
PctI GAATGC 1 cut(s) 781
PdiI GCCGGC 2 cut(s) 153, 331
PdmI GAANNNNTTC 1 cut(s) 401
PfeI GAWTC 2 cut(s) 371, 389
PkrI GCNGC 5 cut(s) 91, 157, 160, 478, 588
PleI GAGTC 2 cut(s) 486, 545
PmeI GTTTAAAC 1 cut(s) 741
PpsI GAGTC 2 cut(s) 486, 545
Ppu21I YACGTR 1 cut(s) 1099
PshAI GACNNNNGTC 1 cut(s) 278
PshBI ATTAAT 1 cut(s) 1029
Psp6I CCWGG 1 cut(s) 365
PspGI CCWGG 1 cut(s) 365
PspN4I GGNNCC 1 cut(s) 334
PspPI GGNCC 1 cut(s) 91
PstI CTGCAG 1 cut(s) 654
PsuI RGATCY 1 cut(s) 691
RsaI GTAC 2 cut(s) 575, 851
RsaNI GTAC 2 cut(s) 574, 850
SalI GTCGAC 1 cut(s) 533
SapI GCTCTTC 1 cut(s) 164
SaqAI TTAA 9 cut(s) 102, 129, 179, 645, 696, 740, 854, 920, 1029
SatI GCNGC 5 cut(s) 90, 156, 159, 477, 587
Sau3AI GATC 4 cut(s) 345, 622, 691, 1078
Sau96I GGNCC 1 cut(s) 91
SbfI CCTGCAGG 1 cut(s) 654
ScaI AGTACT 2 cut(s) 575, 851
SchI GAGTC 2 cut(s) 486, 545
ScrFI CCNGG 2 cut(s) 367, 673
SdaI CCTGCAGG 1 cut(s) 654
SduI GDGCHC 2 cut(s) 682, 844
SfaNI GCATC 3 cut(s) 294, 1079, 1132
SfcI CTRYAG 1 cut(s) 650
SfuI TTCGAA 1 cut(s) 405
SgrAI CRCCGGYG 1 cut(s) 151
SmlI CTYRAG 1 cut(s) 853
SmoI CTYRAG 1 cut(s) 853
SnaBI TACGTA 1 cut(s) 1099
SpeI ACTAGT 1 cut(s) 97
Sse8387I CCTGCAGG 1 cut(s) 654
Sse9I AATT 5 cut(s) 527, 591, 658, 884, 1030
SspMI CTAG 1 cut(s) 98
StyD4I CCNGG 2 cut(s) 365, 671
TaaI ACNGT 5 cut(s) 220, 451, 634, 746, 900
TaiI ACGT 1 cut(s) 1101
TaqI TCGA 6 cut(s) 405, 534, 549, 665, 700, 870
TaqII GACCGA 1 cut(s) 579
TasI AATT 5 cut(s) 527, 591, 658, 884, 1030
TatI WGTACW 2 cut(s) 573, 849
TauI GCSGC 4 cut(s) 92, 158, 161, 479
TfiI GAWTC 2 cut(s) 371, 389
Tru1I TTAA 9 cut(s) 102, 129, 179, 645, 696, 740, 854, 920, 1029
Tru9I TTAA 9 cut(s) 102, 129, 179, 645, 696, 740, 854, 920, 1029
TscAI CASTG 2 cut(s) 264, 330
TseFI GTSAC 2 cut(s) 271, 610
TseI GCWGC 1 cut(s) 586
Tsp45I GTSAC 2 cut(s) 271, 610
TspDTI ATGAA 3 cut(s) 784, 848, 972
TspGWI ACGGA 3 cut(s) 325, 843, 1024
TspRI CASTG 2 cut(s) 264, 330
Vha464I CTTAAG 1 cut(s) 853
VneI GTGCAC 1 cut(s) 840
VspI ATTAAT 1 cut(s) 1029
XceI RCATGY 1 cut(s) 360
XcmI CCANNNNNNNNNTGG 3 cut(s) 322, 811, 1033
XmiI GTMKAC 1 cut(s) 534
XmnI GAANNNNTTC 1 cut(s) 401
XspI CTAG 1 cut(s) 98
ZrmI AGTACT 2 cut(s) 575, 851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.