FvH4_2g02770

Protein of unknown function (DUF1517)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0014649
Physical Location & Seq
Reverse (-)
2191689 .. 2193931
2243 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g02770.t1

Sequence Viewer

Length: 1239 bp
ATGGCAACGGCGGCTACTAATTTGCTCTTCCAACCAAGCCCGTTCAAGTGGAAGCTGAATCAGTTCCGTCCCCTTCGTCCTCTTCTTCTTCCCGCCCAACCACTCAAACTCATCAGTACCGTAAAAGCTCAAGACAAATCTCATATATCTCTGCAGTACCATCCTCGTCCAGCTGCCAAAAAGCTTCAACCTTGGGACCTGAAATCATCAAATTCATCCACCACCTCCTATCCGAAATCAAACTCTGTATGGAGATTTGCTGTAAAAGTGACGGCGGGTGTGGTTTTGGGTGCGTTGTTGCTCATGTACAGACCCAAACCGGCAATGGCCGGCTCTCAGGGGCGGGTCGTTGCAACCTGCAATCCCCGTGCTGACAAGGATCCGGACTCGGCTTTCCGGTACAGGGCAGCGGAACTTAGCAGTTTTGTTCGTACATTTTGGGAGACTGCGGGTATTGACCCATCTAAGCCCAAATGGAATGAACATTGGTCTAACCATTGGTACGAGTGGTATAAGAAGCAAGCCAACTACTTCTTGCTATTCCTTGTCATAGCTCATGTTACTTGGGCATGGCGCCATAATCCAGCTACTCTTGTCACCCTTAACATCCAGGTTAATTTATCAAATGCTGGACGAGCGCTCCACTCGGATCTTAATCGGATTGCTGAAACTAATGCAGATACCTCTACTTATAAAGGTGTAATCAGTATTTTGAAAGAGACAGAAGCAACTTTTCTTAAGCATCCTGATTGTTTCATCTCAGCCTGTATATATGCGGTCGACCAATATGATGGGGATTTTGCACGATTTATGTTAAATGAAATATCTCTTGAAGAGAGATATCCAGTTACGAATGTGAAGAACTTAAAAGCGGAAAGCTCAACAAGCCAAAAATCAGCAAATGAAATCCAAGATGAACATATTCGGGAAGATGCTAATCTACTTGATATTTTCAGACGCAACAAATATGACGTTTCCCAGATAGATTTTTCACGGGAAAGCTCAACAAGCCAAATATCAGCAATCGAAACGCAAGATGAATATATGGAGGTTGTAATATTGGTCGTTATTGCCTCGAAGCATGATGATGACTTTCCCAGAGTCATCAAATCCGTAGATGACTCGAAGGAAGCATTGCGATTGCTTACCGTAGATCCTATATATCCATATGTAGTAGAAGTGTTGTGGAGGCCCAAGTCCTCTTCCTCGAAGGGCAGGAGAAGGTTGTGGTTCCGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1517

Protein Analysis

413

Amino Acids

47.2

Weight (kDa)

9.29

Isoelectric Point (pI)

40.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1517 PF07466 201 - 401 4.9e-15 Protein of unknown function (DUF1517)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 693
Acc36I ACCTGC 1 cut(s) 365
AccB1I GGYRCC 1 cut(s) 573
AccI GTMKAC 1 cut(s) 780
AccIII TCCGGA 1 cut(s) 382
AciI CCGC 8 cut(s) 11, 93, 275, 343, 410, 449, 776, 872
AclWI GGATC 4 cut(s) 374, 387, 657, 1148
AcoI YGGCCR 1 cut(s) 327
AcsI RAATTY 1 cut(s) 211
AcyI GRCGYC 1 cut(s) 574
AfaI GTAC 6 cut(s) 118, 158, 308, 401, 433, 503
AfeI AGCGCT 1 cut(s) 639
AfiI CCNNNNNNNGG 1 cut(s) 403
AflII CTTAAG 1 cut(s) 737
AgsI TTSAA 4 cut(s) 46, 188, 715, 833
AjnI CCWGG 1 cut(s) 609
AluBI AGCT 8 cut(s) 55, 128, 173, 184, 554, 587, 879, 1002
AluI AGCT 8 cut(s) 55, 128, 173, 184, 554, 587, 879, 1002
Alw26I GTCTC 2 cut(s) 437, 713
AlwI GGATC 4 cut(s) 374, 387, 657, 1148
Aor13HI TCCGGA 1 cut(s) 382
Aor51HI AGCGCT 1 cut(s) 639
AoxI GGCC 2 cut(s) 327, 1190
ApeKI GCWGC 2 cut(s) 173, 407
ApoI RAATTY 1 cut(s) 211
Asp700I GAANNNNTTC 2 cut(s) 62, 921
AspLEI GCGC 2 cut(s) 576, 640
AspS9I GGNCC 2 cut(s) 196, 1191
AsuHPI GGTGA 1 cut(s) 589
AvaII GGWCC 1 cut(s) 196
BamHI GGATCC 1 cut(s) 379
BanI GGYRCC 1 cut(s) 573
BbvI GCAGC 2 cut(s) 160, 419
BccI CCATC 3 cut(s) 168, 469, 785
BceAI ACGGC 2 cut(s) 24, 288
BciT130I CCWGG 1 cut(s) 611
BcoDI GTCTC 2 cut(s) 437, 713
BfmI CTRYAG 1 cut(s) 152
BfoI RGCGCY 2 cut(s) 577, 641
BfrI CTTAAG 1 cut(s) 737
BfuAI ACCTGC 1 cut(s) 365
BisI GCNGC 3 cut(s) 12, 174, 408
BlsI GCNGC 3 cut(s) 13, 175, 409
Bme1390I CCNGG 1 cut(s) 611
Bme18I GGWCC 1 cut(s) 196
BmgT120I GGNCC 2 cut(s) 196, 1191
BmiI GGNNCC 4 cut(s) 197, 381, 575, 1232
BmrFI CCNGG 1 cut(s) 611
BmsI GCATC 2 cut(s) 751, 922
BpuEI CTTGAG 1 cut(s) 114
BsaBI GATNNNNATC 2 cut(s) 654, 936
BsaHI GRCGYC 1 cut(s) 574
BsaJI CCNNGG 1 cut(s) 191
BsaWI WCCGGW 2 cut(s) 382, 396
BsaXI ACNNNNNCTCC 2 cut(s) 624, 654
Bsc4I CCNNNNNNNGG 1 cut(s) 403
Bse118I RCCGGY 2 cut(s) 319, 329
Bse1I ACTGG 1 cut(s) 845
Bse3DI GCAATG 2 cut(s) 330, 1133
Bse8I GATNNNNATC 2 cut(s) 654, 936
BseAI TCCGGA 1 cut(s) 382
BseBI CCWGG 1 cut(s) 611
BseDI CCNNGG 1 cut(s) 191
BseGI GGATG 4 cut(s) 160, 215, 606, 742
BseJI GATNNNNATC 2 cut(s) 654, 936
BseLI CCNNNNNNNGG 1 cut(s) 403
BseMI GCAATG 2 cut(s) 330, 1133
BseMII CTCAG 2 cut(s) 350, 774
BseNI ACTGG 1 cut(s) 845
BseXI GCAGC 2 cut(s) 160, 419
Bsh1285I CGRYCG 1 cut(s) 780
BshFI GGCC 2 cut(s) 329, 1192
BshNI GGYRCC 1 cut(s) 573
BsiEI CGRYCG 1 cut(s) 780
BsiSI CCGG 4 cut(s) 320, 330, 383, 397
BslFI GGGAC 2 cut(s) 54, 209
BslI CCNNNNNNNGG 1 cut(s) 403
BsmAI GTCTC 2 cut(s) 437, 713
BsmFI GGGAC 2 cut(s) 54, 209
BsnI GGCC 2 cut(s) 329, 1192
Bsp13I TCCGGA 1 cut(s) 382
Bsp1407I TGTACA 1 cut(s) 306
Bsp143I GATC 3 cut(s) 379, 649, 1153
BspACI CCGC 8 cut(s) 11, 93, 275, 343, 410, 449, 776, 872
BspANI GGCC 2 cut(s) 329, 1192
BspCNI CTCAG 2 cut(s) 349, 773
BspEI TCCGGA 1 cut(s) 382
BspLI GGNNCC 4 cut(s) 197, 381, 575, 1232
BspMAI CTGCAG 1 cut(s) 156
BspMI ACCTGC 1 cut(s) 365
BspPI GGATC 4 cut(s) 374, 387, 657, 1148
BspQI GCTCTTC 1 cut(s) 32
BspT107I GGYRCC 1 cut(s) 573
BspTI CTTAAG 1 cut(s) 737
BsrDI GCAATG 2 cut(s) 330, 1133
BsrFI RCCGGY 2 cut(s) 319, 329
BsrGI TGTACA 1 cut(s) 306
BsrI ACTGG 1 cut(s) 845
BssAI RCCGGY 2 cut(s) 319, 329
BssECI CCNNGG 1 cut(s) 191
BssMI GATC 3 cut(s) 379, 649, 1153
BssNI GRCGYC 1 cut(s) 574
BssT1I CCWWGG 1 cut(s) 191
Bst2UI CCWGG 1 cut(s) 611
Bst4CI ACNGT 2 cut(s) 121, 1150
Bst6I CTCTTC 4 cut(s) 32, 87, 828, 1207
BstACI GRCGYC 1 cut(s) 574
BstAFI CTTAAG 1 cut(s) 737
BstAUI TGTACA 1 cut(s) 306
BstC8I GCNNGC 2 cut(s) 331, 522
BstDEI CTNAG 4 cut(s) 336, 416, 465, 760
BstF5I GGATG 4 cut(s) 160, 215, 606, 742
BstH2I RGCGCY 2 cut(s) 577, 641
BstHHI GCGC 2 cut(s) 576, 640
BstKTI GATC 3 cut(s) 382, 652, 1156
BstMAI GTCTC 2 cut(s) 437, 713
BstMBI GATC 3 cut(s) 379, 649, 1153
BstMCI CGRYCG 1 cut(s) 780
BstMWI GCNNNNNNNGC 4 cut(s) 11, 635, 885, 1008
BstNI CCWGG 1 cut(s) 611
BstSCI CCNGG 1 cut(s) 609
BstSFI CTRYAG 1 cut(s) 152
BstV1I GCAGC 2 cut(s) 160, 419
BstX2I RGATCY 3 cut(s) 379, 649, 1153
BstXI CCANNNNNNTGG 1 cut(s) 791
BstYI RGATCY 3 cut(s) 379, 649, 1153
BsuRI GGCC 2 cut(s) 329, 1192
BtsCI GGATG 4 cut(s) 160, 215, 606, 742
BveI ACCTGC 1 cut(s) 365
Cac8I GCNNGC 2 cut(s) 331, 522
CfoI GCGC 2 cut(s) 576, 640
Cfr10I RCCGGY 2 cut(s) 319, 329
Cfr13I GGNCC 2 cut(s) 196, 1191
CseI GACGC 1 cut(s) 966
Csp6I GTAC 6 cut(s) 117, 157, 307, 400, 432, 502
CviAII CATG 4 cut(s) 304, 557, 570, 1082
CviQI GTAC 6 cut(s) 117, 157, 307, 400, 432, 502
DdeI CTNAG 4 cut(s) 336, 416, 465, 760
DinI GGCGCC 1 cut(s) 575
DpnI GATC 3 cut(s) 381, 651, 1155
DpnII GATC 3 cut(s) 379, 649, 1153
EaeI YGGCCR 1 cut(s) 327
Eam1104I CTCTTC 4 cut(s) 32, 87, 828, 1207
EarI CTCTTC 4 cut(s) 32, 87, 828, 1207
Eco130I CCWWGG 1 cut(s) 191
Eco32I GATATC 1 cut(s) 842
Eco47I GGWCC 1 cut(s) 196
Eco47III AGCGCT 1 cut(s) 639
EcoO109I RGGNCCY 1 cut(s) 196
EcoRII CCWGG 1 cut(s) 609
EcoRV GATATC 1 cut(s) 842
EcoT14I CCWWGG 1 cut(s) 191
EgeI GGCGCC 1 cut(s) 575
EheI GGCGCC 1 cut(s) 575
ErhI CCWWGG 1 cut(s) 191
FaeI CATG 4 cut(s) 307, 560, 573, 1085
FaqI GGGAC 2 cut(s) 54, 209
FatI CATG 4 cut(s) 303, 556, 569, 1081
FauI CCCGC 4 cut(s) 100, 268, 336, 442
FauNDI CATATG 1 cut(s) 1168
FblI GTMKAC 1 cut(s) 780
Fnu4HI GCNGC 3 cut(s) 12, 174, 408
FokI GGATG 4 cut(s) 147, 202, 593, 729
Fsp4HI GCNGC 3 cut(s) 12, 174, 408
GlaI GCGC 2 cut(s) 575, 639
GluI GCNGC 3 cut(s) 12, 174, 408
HaeII RGCGCY 2 cut(s) 577, 641
HaeIII GGCC 2 cut(s) 329, 1192
HapII CCGG 4 cut(s) 320, 330, 383, 397
HgaI GACGC 1 cut(s) 966
HhaI GCGC 2 cut(s) 576, 640
Hin1I GRCGYC 1 cut(s) 574
Hin1II CATG 4 cut(s) 307, 560, 573, 1085
Hin6I GCGC 2 cut(s) 574, 638
HinP1I GCGC 2 cut(s) 574, 638
HincII GTYRAC 1 cut(s) 781
HindII GTYRAC 1 cut(s) 781
HindIII AAGCTT 1 cut(s) 182
HinfI GANTC 4 cut(s) 58, 386, 1101, 1121
HpaII CCGG 4 cut(s) 320, 330, 383, 397
HphI GGTGA 1 cut(s) 589
Hpy166II GTNNAC 1 cut(s) 781
Hpy188I TCNGA 5 cut(s) 234, 649, 660, 956, 1235
Hpy188III TCNNGA 5 cut(s) 131, 383, 746, 830, 926
Hpy8I GTNNAC 1 cut(s) 781
HpyAV CCTTC 4 cut(s) 83, 1120, 1204, 1215
HpyCH4III ACNGT 2 cut(s) 121, 1150
HpyCH4IV ACGT 1 cut(s) 972
HpyCH4V TGCA 5 cut(s) 154, 353, 360, 677, 803
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 635, 885, 1008
HpyF3I CTNAG 4 cut(s) 336, 416, 465, 760
HpySE526I ACGT 1 cut(s) 972
Hsp92I GRCGYC 1 cut(s) 574
Hsp92II CATG 4 cut(s) 307, 560, 573, 1085
HspAI GCGC 2 cut(s) 574, 638
KasI GGCGCC 1 cut(s) 573
Kpn2I TCCGGA 1 cut(s) 382
KroI GCCGGC 1 cut(s) 329
KroNI GCCGGC 1 cut(s) 331
Kzo9I GATC 3 cut(s) 379, 649, 1153
LguI GCTCTTC 1 cut(s) 32
LmnI GCTCC 1 cut(s) 645
Lsp1109I GCAGC 2 cut(s) 160, 419
LweI GCATC 2 cut(s) 751, 922
MaeII ACGT 1 cut(s) 972
MaeIII GTNAC 4 cut(s) 268, 559, 595, 847
MalI GATC 3 cut(s) 381, 651, 1155
MboI GATC 3 cut(s) 379, 649, 1153
MboII GAAGA 8 cut(s) 19, 74, 77, 80, 845, 871, 941, 1194
MflI RGATCY 3 cut(s) 379, 649, 1153
MluCI AATT 3 cut(s) 19, 211, 616
Mly113I GGCGCC 1 cut(s) 574
MlyI GAGTC 3 cut(s) 380, 1110, 1115
MmeI TCCRAC 1 cut(s) 55
MnlI CCTC 9 cut(s) 90, 174, 235, 694, 1042, 1084, 1182, 1210, 1216
MroI TCCGGA 1 cut(s) 382
MroNI GCCGGC 1 cut(s) 329
MroXI GAANNNNTTC 2 cut(s) 62, 921
MseI TTAA 6 cut(s) 603, 615, 654, 738, 815, 866
MslI CAYNNNNRTG 1 cut(s) 1086
MspA1I CMGCKG 2 cut(s) 173, 410
MspCI CTTAAG 1 cut(s) 737
MspI CCGG 4 cut(s) 320, 330, 383, 397
MspR9I CCNGG 1 cut(s) 611
MvaI CCWGG 1 cut(s) 611
MwoI GCNNNNNNNGC 4 cut(s) 11, 635, 885, 1008
NaeI GCCGGC 1 cut(s) 331
NarI GGCGCC 1 cut(s) 574
NdeI CATATG 1 cut(s) 1168
NdeII GATC 3 cut(s) 379, 649, 1153
NgoMIV GCCGGC 1 cut(s) 329
NlaIII CATG 4 cut(s) 307, 560, 573, 1085
NlaIV GGNNCC 4 cut(s) 197, 381, 575, 1232
NmeAIII GCCGAG 1 cut(s) 368
NmuCI GTSAC 2 cut(s) 268, 595
PciSI GCTCTTC 1 cut(s) 32
PdiI GCCGGC 1 cut(s) 331
PdmI GAANNNNTTC 2 cut(s) 62, 921
PfeI GAWTC 1 cut(s) 58
PkrI GCNGC 3 cut(s) 13, 175, 409
PleI GAGTC 3 cut(s) 380, 1109, 1115
PluTI GGCGCC 1 cut(s) 577
PpsI GAGTC 3 cut(s) 380, 1109, 1115
PpuMI RGGWCCY 1 cut(s) 196
PsiI TTATAA 1 cut(s) 693
Psp5II RGGWCCY 1 cut(s) 196
Psp6I CCWGG 1 cut(s) 609
PspGI CCWGG 1 cut(s) 609
PspN4I GGNNCC 4 cut(s) 197, 381, 575, 1232
PspPI GGNCC 2 cut(s) 196, 1191
PspPPI RGGWCCY 1 cut(s) 196
PstI CTGCAG 1 cut(s) 156
PsuI RGATCY 3 cut(s) 379, 649, 1153
PvuII CAGCTG 1 cut(s) 173
RsaI GTAC 6 cut(s) 118, 158, 308, 401, 433, 503
RsaNI GTAC 6 cut(s) 117, 157, 307, 400, 432, 502
RseI CAYNNNNRTG 1 cut(s) 1086
SalI GTCGAC 1 cut(s) 779
SapI GCTCTTC 1 cut(s) 32
SaqAI TTAA 6 cut(s) 603, 615, 654, 738, 815, 866
SatI GCNGC 3 cut(s) 12, 174, 408
Sau3AI GATC 3 cut(s) 379, 649, 1153
Sau96I GGNCC 2 cut(s) 196, 1191
SchI GAGTC 3 cut(s) 380, 1110, 1115
ScrFI CCNGG 1 cut(s) 611
SfaNI GCATC 2 cut(s) 751, 922
SfcI CTRYAG 1 cut(s) 152
SfoI GGCGCC 1 cut(s) 575
SinI GGWCC 1 cut(s) 196
SmiMI CAYNNNNRTG 1 cut(s) 1086
SmlI CTYRAG 2 cut(s) 129, 737
SmoI CTYRAG 2 cut(s) 129, 737
Sse9I AATT 3 cut(s) 19, 211, 616
SsiI CCGC 8 cut(s) 11, 93, 275, 343, 410, 449, 776, 872
SspDI GGCGCC 1 cut(s) 573
SspI AATATT 1 cut(s) 1059
StyD4I CCNGG 1 cut(s) 609
StyI CCWWGG 1 cut(s) 191
TaaI ACNGT 2 cut(s) 121, 1150
TaiI ACGT 1 cut(s) 975
TaqI TCGA 5 cut(s) 780, 1026, 1076, 1124, 1208
TasI AATT 3 cut(s) 19, 211, 616
TatI WGTACW 1 cut(s) 306
TauI GCSGC 1 cut(s) 14
TfiI GAWTC 1 cut(s) 58
Tru1I TTAA 6 cut(s) 603, 615, 654, 738, 815, 866
Tru9I TTAA 6 cut(s) 603, 615, 654, 738, 815, 866
TseFI GTSAC 2 cut(s) 268, 595
TseI GCWGC 2 cut(s) 173, 407
Tsp45I GTSAC 2 cut(s) 268, 595
TspDTI ATGAA 7 cut(s) 204, 495, 745, 834, 918, 930, 1053
TspGWI ACGGA 2 cut(s) 56, 1102
Vha464I CTTAAG 1 cut(s) 737
VpaK11BI GGWCC 1 cut(s) 196
XapI RAATTY 1 cut(s) 211
XcmI CCANNNNNNNNNTGG 1 cut(s) 322
XmiI GTMKAC 1 cut(s) 780
XmnI GAANNNNTTC 2 cut(s) 62, 921
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.