FvH4_2g02100

ER lumen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009847
Physical Location & Seq
Forward (+)
1762659 .. 1765655
2997 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g02100.t1

Sequence Viewer

Length: 819 bp
ATGAGGGCCCCGAAGACGCCGATCAACACCGTGTCGACATGGGTCCGGCGCCAACCGCCGAAAGTGAAAGGCTTTCTGGCCGTGGTGGCGGGCATGGCGGCTCTGGTTTTGCTCCGATTCGTCGTTCACGATCATGATAACCTCTTCGTCGCAGCTGAGGCTGTCCACTCCGGCGGAATCTTAGTCCTCATCTATAAGCTCATGAAGGAGAAGACTTGCGCCGGGCTGTCACTGAAATCGCAGGAGCTTACAGCAATGTTCTTAGCTGTGAGATTGTACTGCAGTGTTGTTATGGAATATGACATACACACTTTGCTTGATTTAGCTACACTGGTGACCACCCTCTGGGTTATTTATATGATCCGGTTTAATTTGAAGTCTAGTTATATGGAGGACAAAGACAACTTTGCAATATACTATGTGGTGGCACCATGTGCTCTACTAGCTTTGTTTATACATCCGTCAACTTCTCATCATTTATTGAACAGGATTTCCTGGGCCTTTTGTGTATATTTAGAAGCTGTTTCAGTACTGCCCCAGTTGAGGGTGATGCAGAATACAAAGATTGTTGAGCCATTCACAGCTCATTACGTGTTTGCACTTGGTATTGCAAGGTTCCTGAGCTGTGCCCATTGGGTTCTCCAGGTATTGGATACTCGCGGACACTTGCTCGTGGCCTTGGGGTATGGGTTGTGGCCTTCTATGGTTCTTATTTCGGAAATCGTCCAGACTTTCATATTAGCAGACTTCTGCTACTACTATGTCAAAAGTGTTTTCGGAGGACAGCTTGTTCTTCGTCTTCCTTCTGGAGTGGTATGA

Protein Analysis

273

Amino Acids

30.62

Weight (kDa)

8.98

Isoelectric Point (pI)

30.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ER_lumen_recept PF00810 72 - 214 2e-44 ER lumen protein retaining receptor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 48, 427
AccB7I CCANNNNNTGG 2 cut(s) 345, 649
AccI GTMKAC 1 cut(s) 35
AccII CGCG 1 cut(s) 660
AciI CCGC 5 cut(s) 56, 89, 98, 174, 660
AclWI GGATC 1 cut(s) 355
AcoI YGGCCR 1 cut(s) 78
AcyI GRCGYC 2 cut(s) 17, 49
AdeI CACNNNGTG 1 cut(s) 434
AfaI GTAC 2 cut(s) 278, 531
AfiI CCNNNNNNNGG 4 cut(s) 345, 543, 544, 649
AflIII ACRYGT 1 cut(s) 591
AgsI TTSAA 2 cut(s) 376, 484
AjnI CCWGG 2 cut(s) 494, 642
AloI GAACNNNNNNTCC 4 cut(s) 476, 508, 774, 806
Alw21I GWGCWC 1 cut(s) 439
AlwI GGATC 1 cut(s) 355
AoxI GGCC 5 cut(s) 6, 78, 498, 675, 695
ApaI GGGCCC 1 cut(s) 10
ApeKI GCWGC 1 cut(s) 152
AspLEI GCGC 2 cut(s) 51, 221
AspS9I GGNCC 4 cut(s) 6, 7, 43, 498
AsuC2I CCSGG 1 cut(s) 223
AsuHPI GGTGA 2 cut(s) 346, 559
AvaII GGWCC 1 cut(s) 43
BaeGI GKGCMC 2 cut(s) 10, 631
BanI GGYRCC 2 cut(s) 48, 427
BanII GRGCYC 1 cut(s) 10
BauI CACGAG 1 cut(s) 671
BbsI GAAGAC 3 cut(s) 20, 218, 791
Bbv12I GWGCWC 1 cut(s) 439
BbvCI CCTCAGC 1 cut(s) 156
BbvI GCAGC 1 cut(s) 164
BceAI ACGGC 1 cut(s) 65
BciT130I CCWGG 2 cut(s) 496, 644
BciVI GTATCC 1 cut(s) 646
BcnI CCSGG 1 cut(s) 223
BfaI CTAG 2 cut(s) 381, 443
BfmI CTRYAG 1 cut(s) 280
BfoI RGCGCY 1 cut(s) 52
BfuI GTATCC 1 cut(s) 646
BglI GCCNNNNNGGC 1 cut(s) 86
BisI GCNGC 2 cut(s) 99, 153
BlsI GCNGC 2 cut(s) 100, 154
BmcAI AGTACT 1 cut(s) 531
Bme1390I CCNGG 3 cut(s) 223, 496, 644
Bme18I GGWCC 1 cut(s) 43
BmgT120I GGNCC 4 cut(s) 6, 7, 43, 498
BmiI GGNNCC 6 cut(s) 8, 9, 44, 50, 429, 617
BmrFI CCNGG 3 cut(s) 223, 496, 644
BmrI ACTGGG 1 cut(s) 532
BmsI GCATC 1 cut(s) 540
BmuI ACTGGG 1 cut(s) 532
BoxI GACNNNNGTC 1 cut(s) 41
BpiI GAAGAC 3 cut(s) 20, 218, 791
BpmI CTGGAG 1 cut(s) 626
Bpu10I CCTNAGC 2 cut(s) 156, 620
BpuMI CCSGG 1 cut(s) 223
BsaAI YACGTR 1 cut(s) 592
BsaHI GRCGYC 2 cut(s) 17, 49
BsaJI CCNNGG 3 cut(s) 81, 495, 678
BsaWI WCCGGW 1 cut(s) 363
Bsc4I CCNNNNNNNGG 4 cut(s) 345, 543, 544, 649
Bse1I ACTGG 2 cut(s) 336, 538
Bse3DI GCAATG 1 cut(s) 261
BseBI CCWGG 2 cut(s) 496, 644
BseDI CCNNGG 3 cut(s) 81, 495, 678
BseGI GGATG 1 cut(s) 457
BseLI CCNNNNNNNGG 4 cut(s) 345, 543, 544, 649
BseMI GCAATG 1 cut(s) 261
BseMII CTCAG 2 cut(s) 147, 611
BseNI ACTGG 2 cut(s) 336, 538
BseSI GKGCMC 2 cut(s) 10, 631
BseXI GCAGC 1 cut(s) 164
Bsh1236I CGCG 1 cut(s) 660
BshFI GGCC 5 cut(s) 8, 80, 500, 677, 697
BshNI GGYRCC 2 cut(s) 48, 427
BsiHKAI GWGCWC 1 cut(s) 439
BsiSI CCGG 4 cut(s) 46, 171, 222, 364
BslI CCNNNNNNNGG 4 cut(s) 345, 543, 544, 649
BsnI GGCC 5 cut(s) 8, 80, 500, 677, 697
Bsp120I GGGCCC 1 cut(s) 6
Bsp1286I GDGCHC 3 cut(s) 10, 439, 631
Bsp143I GATC 3 cut(s) 21, 130, 360
BspACI CCGC 5 cut(s) 56, 89, 98, 174, 660
BspANI GGCC 5 cut(s) 8, 80, 500, 677, 697
BspCNI CTCAG 2 cut(s) 148, 612
BspFNI CGCG 1 cut(s) 660
BspHI TCATGA 2 cut(s) 133, 201
BspLI GGNNCC 6 cut(s) 8, 9, 44, 50, 429, 617
BspMAI CTGCAG 1 cut(s) 284
BspPI GGATC 1 cut(s) 355
BspT107I GGYRCC 2 cut(s) 48, 427
BsrDI GCAATG 1 cut(s) 261
BsrI ACTGG 2 cut(s) 336, 538
BssECI CCNNGG 3 cut(s) 81, 495, 678
BssMI GATC 3 cut(s) 21, 130, 360
BssNI GRCGYC 2 cut(s) 17, 49
BssSI CACGAG 1 cut(s) 671
BssT1I CCWWGG 1 cut(s) 678
Bst2BI CACGAG 1 cut(s) 671
Bst2UI CCWGG 2 cut(s) 496, 644
Bst4CI ACNGT 1 cut(s) 31
Bst6I CTCTTC 1 cut(s) 149
BstACI GRCGYC 2 cut(s) 17, 49
BstAPI GCANNNNNTGC 1 cut(s) 434
BstBAI YACGTR 1 cut(s) 592
BstC8I GCNNGC 1 cut(s) 91
BstDEI CTNAG 4 cut(s) 156, 181, 262, 620
BstDSI CCRYGG 1 cut(s) 81
BstEII GGTNACC 1 cut(s) 334
BstF5I GGATG 1 cut(s) 457
BstFNI CGCG 1 cut(s) 660
BstH2I RGCGCY 1 cut(s) 52
BstHHI GCGC 2 cut(s) 51, 221
BstKTI GATC 3 cut(s) 24, 133, 363
BstMBI GATC 3 cut(s) 21, 130, 360
BstMWI GCNNNNNNNGC 6 cut(s) 55, 86, 95, 158, 434, 443
BstNI CCWGG 2 cut(s) 496, 644
BstPAI GACNNNNGTC 1 cut(s) 41
BstPI GGTNACC 1 cut(s) 334
BstSCI CCNGG 3 cut(s) 221, 494, 642
BstSFI CTRYAG 1 cut(s) 280
BstSLI GKGCMC 2 cut(s) 10, 631
BstUI CGCG 1 cut(s) 660
BstV1I GCAGC 1 cut(s) 164
BstV2I GAAGAC 3 cut(s) 20, 218, 791
BsuI GTATCC 1 cut(s) 646
BsuRI GGCC 5 cut(s) 8, 80, 500, 677, 697
BtgI CCRYGG 1 cut(s) 81
BtsCI GGATG 1 cut(s) 457
BtsI GCAGTG 1 cut(s) 289
BtsIMutI CAGTG 3 cut(s) 230, 289, 329
Cac8I GCNNGC 1 cut(s) 91
CciI TCATGA 2 cut(s) 133, 201
CfoI GCGC 2 cut(s) 51, 221
Cfr13I GGNCC 4 cut(s) 6, 7, 43, 498
CseI GACGC 1 cut(s) 25
Csp6I GTAC 2 cut(s) 277, 530
CviAII CATG 5 cut(s) 39, 94, 134, 202, 432
CviQI GTAC 2 cut(s) 277, 530
DdeI CTNAG 4 cut(s) 156, 181, 262, 620
DinI GGCGCC 1 cut(s) 50
DpnI GATC 3 cut(s) 23, 132, 362
DpnII GATC 3 cut(s) 21, 130, 360
DraIII CACNNNGTG 1 cut(s) 434
EaeI YGGCCR 1 cut(s) 78
Eam1104I CTCTTC 1 cut(s) 149
EarI CTCTTC 1 cut(s) 149
EciI GGCGGA 1 cut(s) 189
Eco130I CCWWGG 1 cut(s) 678
Eco24I GRGCYC 1 cut(s) 10
Eco47I GGWCC 1 cut(s) 43
Eco91I GGTNACC 1 cut(s) 334
EcoO109I RGGNCCY 2 cut(s) 6, 7
EcoO65I GGTNACC 1 cut(s) 334
EcoRII CCWGG 2 cut(s) 494, 642
EcoT14I CCWWGG 1 cut(s) 678
EcoT38I GRGCYC 1 cut(s) 10
EgeI GGCGCC 1 cut(s) 50
EheI GGCGCC 1 cut(s) 50
ErhI CCWWGG 1 cut(s) 678
FaeI CATG 5 cut(s) 42, 97, 137, 205, 435
FatI CATG 5 cut(s) 38, 93, 133, 201, 431
FauI CCCGC 1 cut(s) 82
FblI GTMKAC 1 cut(s) 35
Fnu4HI GCNGC 2 cut(s) 99, 153
FokI GGATG 1 cut(s) 444
FriOI GRGCYC 1 cut(s) 10
Fsp4HI GCNGC 2 cut(s) 99, 153
FspBI CTAG 2 cut(s) 381, 443
GlaI GCGC 2 cut(s) 50, 220
GluI GCNGC 2 cut(s) 99, 153
GsuI CTGGAG 1 cut(s) 626
HaeII RGCGCY 1 cut(s) 52
HaeIII GGCC 5 cut(s) 8, 80, 500, 677, 697
HapII CCGG 4 cut(s) 46, 171, 222, 364
HgaI GACGC 1 cut(s) 25
HhaI GCGC 2 cut(s) 51, 221
Hin1I GRCGYC 2 cut(s) 17, 49
Hin1II CATG 5 cut(s) 42, 97, 137, 205, 435
Hin6I GCGC 2 cut(s) 49, 219
HinP1I GCGC 2 cut(s) 49, 219
HincII GTYRAC 2 cut(s) 36, 465
HindII GTYRAC 2 cut(s) 36, 465
HinfI GANTC 2 cut(s) 117, 177
HpaII CCGG 4 cut(s) 46, 171, 222, 364
HphI GGTGA 2 cut(s) 346, 559
Hpy166II GTNNAC 4 cut(s) 36, 127, 166, 465
Hpy188I TCNGA 3 cut(s) 116, 718, 779
Hpy188III TCNNGA 6 cut(s) 128, 134, 202, 619, 727, 807
Hpy8I GTNNAC 4 cut(s) 36, 127, 166, 465
Hpy99I CGWCG 2 cut(s) 125, 152
HpyAV CCTTC 3 cut(s) 199, 708, 813
HpyCH4III ACNGT 1 cut(s) 31
HpyCH4IV ACGT 1 cut(s) 591
HpyCH4V TGCA 5 cut(s) 282, 410, 553, 599, 611
HpyF10VI GCNNNNNNNGC 6 cut(s) 55, 86, 95, 158, 434, 443
HpyF3I CTNAG 4 cut(s) 156, 181, 262, 620
HpySE526I ACGT 1 cut(s) 591
Hsp92I GRCGYC 2 cut(s) 17, 49
Hsp92II CATG 5 cut(s) 42, 97, 137, 205, 435
HspAI GCGC 2 cut(s) 49, 219
KasI GGCGCC 1 cut(s) 48
Kzo9I GATC 3 cut(s) 21, 130, 360
LmnI GCTCC 2 cut(s) 117, 244
Lsp1109I GCAGC 1 cut(s) 164
LweI GCATC 1 cut(s) 540
MaeI CTAG 2 cut(s) 381, 443
MaeII ACGT 1 cut(s) 591
MaeIII GTNAC 2 cut(s) 228, 334
MalI GATC 3 cut(s) 23, 132, 362
MboI GATC 3 cut(s) 21, 130, 360
MboII GAAGA 5 cut(s) 25, 136, 223, 785, 791
MhlI GDGCHC 3 cut(s) 10, 439, 631
MluCI AATT 1 cut(s) 370
Mly113I GGCGCC 1 cut(s) 49
MnlI CCTC 7 cut(s) 151, 152, 197, 353, 385, 537, 773
MseI TTAA 1 cut(s) 369
MslI CAYNNNNRTG 1 cut(s) 132
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 4 cut(s) 46, 171, 222, 364
MspR9I CCNGG 3 cut(s) 223, 496, 644
MvaI CCWGG 2 cut(s) 496, 644
MvnI CGCG 1 cut(s) 660
MwoI GCNNNNNNNGC 6 cut(s) 55, 86, 95, 158, 434, 443
NarI GGCGCC 1 cut(s) 49
NciI CCSGG 1 cut(s) 223
NdeII GATC 3 cut(s) 21, 130, 360
NlaIII CATG 5 cut(s) 42, 97, 137, 205, 435
NlaIV GGNNCC 6 cut(s) 8, 9, 44, 50, 429, 617
NmuCI GTSAC 2 cut(s) 228, 334
PagI TCATGA 2 cut(s) 133, 201
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PfeI GAWTC 2 cut(s) 117, 177
PflMI CCANNNNNTGG 2 cut(s) 345, 649
PkrI GCNGC 2 cut(s) 100, 154
PluTI GGCGCC 1 cut(s) 52
Ppu21I YACGTR 1 cut(s) 592
PshAI GACNNNNGTC 1 cut(s) 41
Psp6I CCWGG 2 cut(s) 494, 642
PspEI GGTNACC 1 cut(s) 334
PspGI CCWGG 2 cut(s) 494, 642
PspN4I GGNNCC 6 cut(s) 8, 9, 44, 50, 429, 617
PspOMI GGGCCC 1 cut(s) 6
PspPI GGNCC 4 cut(s) 6, 7, 43, 498
PstI CTGCAG 1 cut(s) 284
PvuII CAGCTG 1 cut(s) 155
RsaI GTAC 2 cut(s) 278, 531
RsaNI GTAC 2 cut(s) 277, 530
RseI CAYNNNNRTG 1 cut(s) 132
SalI GTCGAC 1 cut(s) 34
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 2 cut(s) 99, 153
Sau3AI GATC 3 cut(s) 21, 130, 360
Sau96I GGNCC 4 cut(s) 6, 7, 43, 498
ScaI AGTACT 1 cut(s) 531
ScrFI CCNGG 3 cut(s) 223, 496, 644
SduI GDGCHC 3 cut(s) 10, 439, 631
SfaNI GCATC 1 cut(s) 540
SfcI CTRYAG 1 cut(s) 280
SfoI GGCGCC 1 cut(s) 50
SinI GGWCC 1 cut(s) 43
SmiMI CAYNNNNRTG 1 cut(s) 132
Sse9I AATT 1 cut(s) 370
SsiI CCGC 5 cut(s) 56, 89, 98, 174, 660
SspDI GGCGCC 1 cut(s) 48
SspMI CTAG 2 cut(s) 381, 443
StyD4I CCNGG 3 cut(s) 221, 494, 642
StyI CCWWGG 1 cut(s) 678
TaaI ACNGT 1 cut(s) 31
TaiI ACGT 1 cut(s) 594
TaqI TCGA 1 cut(s) 35
TasI AATT 1 cut(s) 370
TatI WGTACW 2 cut(s) 276, 529
TauI GCSGC 1 cut(s) 101
TfiI GAWTC 2 cut(s) 117, 177
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 3 cut(s) 237, 289, 336
TseFI GTSAC 2 cut(s) 228, 334
TseI GCWGC 1 cut(s) 152
Tsp45I GTSAC 2 cut(s) 228, 334
TspDTI ATGAA 2 cut(s) 218, 724
TspGWI ACGGA 1 cut(s) 450
TspRI CASTG 3 cut(s) 237, 289, 336
Van91I CCANNNNNTGG 2 cut(s) 345, 649
VpaK11BI GGWCC 1 cut(s) 43
XmiI GTMKAC 1 cut(s) 35
XspI CTAG 2 cut(s) 381, 443
ZrmI AGTACT 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.