RchiOBHm_Chr6g0245611

ER lumen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0009847
Physical Location & Seq
Forward (+)
1390494 .. 1393720
3227 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ22012

Sequence Viewer

Length: 819 bp
ATGAGGGCTCCGAAGACGCCGATCAACACCGTGTCGACATGGGTCCGGCGCCAACCGCCGAAAGTGAAGGGCTTTCTGGCCGTGGTGACCGGCATGGCGACTCTGGTTCTGCTCCGATTCGTCGTTCACGATCACGATAACCTCTTCGTCGCAGCTGAGGCTGTGCACTCCGCCGGAATCTCCGTCCTCATCTATAAGCTCATGAAGGAGAAGACTTGCGCCGGGCTATCACTCAAATCGCAGGAACTTACAGCTATGTTTTTAGCTGTGAGATTGTACTGCAGTGTGGTTATGGAATATGACATACACACCCTGCTTGATTTAGCTACACTGGTGACCACCCTCTGGGTTATTTATATGATCCGCTTTAACTTGAAGTCTAGTTACATGGAGGACAAAGACAACTTTGCAATATACTATGTGGTTGCACCATGTGCTCTACTAGCTTTATTTATACATCCGTCAACTTCTCATCATTTGTTGAACAGGATTTCCTGGGCCTTTTGTGTGTATCTAGAAGCTGTTTCAGTACTGCCCCAGTTGAGGGTCATGCAGAATACAAAGATTGTTGAGCCATTCACAGCTCATTACGTGTTTGCACTTGGTGTTGCAAGGTTCCTGAGCTGTGCCCATTGGGTTCTCCAGGTATTAGATACTCGCGGACATTTGCTGGTGGCCTTGGGGTATGGGTTGTGGCCTTCTATGGTTCTTATTTCGGAAATCGTCCAAACTTTCATATTAGCAGACTTCTGTTACTACTATGTCAAAAGTGTTTTCGGAGGGCAGCTTGTTCTTCGTCTCCCTTCTGGAGTTGTATGA

Protein Analysis

272

Amino Acids

30.65

Weight (kDa)

8.98

Isoelectric Point (pI)

29.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ER_lumen_recept PF00810 72 - 214 1.5e-44 ER lumen protein retaining receptor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 48
AccB7I CCANNNNNTGG 1 cut(s) 345
AccI GTMKAC 1 cut(s) 35
AccII CGCG 1 cut(s) 660
AciI CCGC 4 cut(s) 56, 171, 364, 660
AclWI GGATC 1 cut(s) 355
AcoI YGGCCR 1 cut(s) 78
AcyI GRCGYC 2 cut(s) 17, 49
AdeI CACNNNGTG 2 cut(s) 434, 605
AfaI GTAC 2 cut(s) 278, 531
AfiI CCNNNNNNNGG 3 cut(s) 345, 543, 544
AflIII ACRYGT 1 cut(s) 591
AgsI TTSAA 2 cut(s) 376, 484
AjnI CCWGG 2 cut(s) 494, 642
AloI GAACNNNNNNTCC 2 cut(s) 476, 508
Alw21I GWGCWC 2 cut(s) 168, 439
Alw26I GTCTC 1 cut(s) 803
Alw44I GTGCAC 1 cut(s) 164
AlwI GGATC 1 cut(s) 355
AoxI GGCC 4 cut(s) 78, 498, 675, 695
ApaLI GTGCAC 1 cut(s) 164
ApeKI GCWGC 2 cut(s) 152, 784
AspLEI GCGC 2 cut(s) 51, 221
AspS9I GGNCC 2 cut(s) 43, 498
AsuC2I CCSGG 1 cut(s) 223
AsuHPI GGTGA 2 cut(s) 97, 346
AvaII GGWCC 1 cut(s) 43
BaeGI GKGCMC 2 cut(s) 168, 631
BanI GGYRCC 1 cut(s) 48
BanII GRGCYC 1 cut(s) 10
BarI GAAGNNNNNNTAC 2 cut(s) 368, 400
BbsI GAAGAC 2 cut(s) 20, 218
Bbv12I GWGCWC 2 cut(s) 168, 439
BbvCI CCTCAGC 1 cut(s) 156
BbvI GCAGC 2 cut(s) 164, 796
BceAI ACGGC 1 cut(s) 65
BciT130I CCWGG 2 cut(s) 496, 644
BcnI CCSGG 1 cut(s) 223
BcoDI GTCTC 1 cut(s) 803
BfaI CTAG 3 cut(s) 381, 443, 515
BfmI CTRYAG 1 cut(s) 280
BfoI RGCGCY 1 cut(s) 52
BisI GCNGC 2 cut(s) 153, 785
BlsI GCNGC 2 cut(s) 154, 786
BmcAI AGTACT 1 cut(s) 531
Bme1390I CCNGG 3 cut(s) 223, 496, 644
Bme18I GGWCC 1 cut(s) 43
BmgT120I GGNCC 2 cut(s) 43, 498
BmiI GGNNCC 4 cut(s) 9, 44, 50, 617
BmrFI CCNGG 3 cut(s) 223, 496, 644
BmrI ACTGGG 1 cut(s) 532
BmuI ACTGGG 1 cut(s) 532
BoxI GACNNNNGTC 1 cut(s) 41
BpiI GAAGAC 2 cut(s) 20, 218
BpmI CTGGAG 1 cut(s) 626
Bpu10I CCTNAGC 2 cut(s) 156, 620
BpuMI CCSGG 1 cut(s) 223
BsaAI YACGTR 1 cut(s) 592
BsaHI GRCGYC 2 cut(s) 17, 49
BsaJI CCNNGG 3 cut(s) 81, 495, 678
Bsc4I CCNNNNNNNGG 3 cut(s) 345, 543, 544
Bse118I RCCGGY 1 cut(s) 89
Bse1I ACTGG 2 cut(s) 336, 538
BseBI CCWGG 2 cut(s) 496, 644
BseDI CCNNGG 3 cut(s) 81, 495, 678
BseGI GGATG 1 cut(s) 457
BseLI CCNNNNNNNGG 3 cut(s) 345, 543, 544
BseMII CTCAG 2 cut(s) 147, 611
BseNI ACTGG 2 cut(s) 336, 538
BseSI GKGCMC 2 cut(s) 168, 631
BseXI GCAGC 2 cut(s) 164, 796
Bsh1236I CGCG 1 cut(s) 660
BshFI GGCC 4 cut(s) 80, 500, 677, 697
BshNI GGYRCC 1 cut(s) 48
BsiHKAI GWGCWC 2 cut(s) 168, 439
BsiSI CCGG 4 cut(s) 46, 90, 174, 222
BslI CCNNNNNNNGG 3 cut(s) 345, 543, 544
BsmAI GTCTC 1 cut(s) 803
BsmBI CGTCTC 1 cut(s) 803
BsnI GGCC 4 cut(s) 80, 500, 677, 697
Bsp1286I GDGCHC 4 cut(s) 10, 168, 439, 631
Bsp143I GATC 3 cut(s) 21, 130, 360
BspACI CCGC 4 cut(s) 56, 171, 364, 660
BspANI GGCC 4 cut(s) 80, 500, 677, 697
BspCNI CTCAG 2 cut(s) 148, 612
BspFNI CGCG 1 cut(s) 660
BspHI TCATGA 1 cut(s) 201
BspLI GGNNCC 4 cut(s) 9, 44, 50, 617
BspMAI CTGCAG 1 cut(s) 284
BspPI GGATC 1 cut(s) 355
BspT107I GGYRCC 1 cut(s) 48
BsrFI RCCGGY 1 cut(s) 89
BsrI ACTGG 2 cut(s) 336, 538
BssAI RCCGGY 1 cut(s) 89
BssECI CCNNGG 3 cut(s) 81, 495, 678
BssMI GATC 3 cut(s) 21, 130, 360
BssNI GRCGYC 2 cut(s) 17, 49
BssT1I CCWWGG 1 cut(s) 678
Bst2UI CCWGG 2 cut(s) 496, 644
Bst4CI ACNGT 1 cut(s) 31
Bst6I CTCTTC 1 cut(s) 149
BstACI GRCGYC 2 cut(s) 17, 49
BstAPI GCANNNNNTGC 1 cut(s) 434
BstBAI YACGTR 1 cut(s) 592
BstDEI CTNAG 2 cut(s) 156, 620
BstDSI CCRYGG 1 cut(s) 81
BstEII GGTNACC 2 cut(s) 85, 334
BstF5I GGATG 1 cut(s) 457
BstFNI CGCG 1 cut(s) 660
BstH2I RGCGCY 1 cut(s) 52
BstHHI GCGC 2 cut(s) 51, 221
BstKTI GATC 3 cut(s) 24, 133, 363
BstMAI GTCTC 1 cut(s) 803
BstMBI GATC 3 cut(s) 21, 130, 360
BstMWI GCNNNNNNNGC 4 cut(s) 55, 158, 434, 443
BstNI CCWGG 2 cut(s) 496, 644
BstPAI GACNNNNGTC 1 cut(s) 41
BstPI GGTNACC 2 cut(s) 85, 334
BstSCI CCNGG 3 cut(s) 221, 494, 642
BstSFI CTRYAG 1 cut(s) 280
BstSLI GKGCMC 2 cut(s) 168, 631
BstUI CGCG 1 cut(s) 660
BstV1I GCAGC 2 cut(s) 164, 796
BstV2I GAAGAC 2 cut(s) 20, 218
BsuRI GGCC 4 cut(s) 80, 500, 677, 697
BtgI CCRYGG 1 cut(s) 81
BtsCI GGATG 1 cut(s) 457
BtsI GCAGTG 1 cut(s) 289
BtsIMutI CAGTG 2 cut(s) 289, 329
CciI TCATGA 1 cut(s) 201
CfoI GCGC 2 cut(s) 51, 221
Cfr10I RCCGGY 1 cut(s) 89
Cfr13I GGNCC 2 cut(s) 43, 498
CseI GACGC 1 cut(s) 25
Csp6I GTAC 2 cut(s) 277, 530
CviAII CATG 6 cut(s) 39, 94, 202, 388, 432, 550
CviQI GTAC 2 cut(s) 277, 530
DdeI CTNAG 2 cut(s) 156, 620
DinI GGCGCC 1 cut(s) 50
DpnI GATC 3 cut(s) 23, 132, 362
DpnII GATC 3 cut(s) 21, 130, 360
DraIII CACNNNGTG 2 cut(s) 434, 605
EaeI YGGCCR 1 cut(s) 78
Eam1104I CTCTTC 1 cut(s) 149
EarI CTCTTC 1 cut(s) 149
EciI GGCGGA 1 cut(s) 160
Eco130I CCWWGG 1 cut(s) 678
Eco24I GRGCYC 1 cut(s) 10
Eco47I GGWCC 1 cut(s) 43
Eco91I GGTNACC 2 cut(s) 85, 334
EcoO65I GGTNACC 2 cut(s) 85, 334
EcoRII CCWGG 2 cut(s) 494, 642
EcoT14I CCWWGG 1 cut(s) 678
EcoT38I GRGCYC 1 cut(s) 10
EgeI GGCGCC 1 cut(s) 50
EheI GGCGCC 1 cut(s) 50
ErhI CCWWGG 1 cut(s) 678
Esp3I CGTCTC 1 cut(s) 803
FaeI CATG 6 cut(s) 42, 97, 205, 391, 435, 553
FatI CATG 6 cut(s) 38, 93, 201, 387, 431, 549
FblI GTMKAC 1 cut(s) 35
Fnu4HI GCNGC 2 cut(s) 153, 785
FokI GGATG 1 cut(s) 444
FriOI GRGCYC 1 cut(s) 10
Fsp4HI GCNGC 2 cut(s) 153, 785
FspBI CTAG 3 cut(s) 381, 443, 515
GlaI GCGC 2 cut(s) 50, 220
GluI GCNGC 2 cut(s) 153, 785
GsuI CTGGAG 1 cut(s) 626
HaeII RGCGCY 1 cut(s) 52
HaeIII GGCC 4 cut(s) 80, 500, 677, 697
HapII CCGG 4 cut(s) 46, 90, 174, 222
HgaI GACGC 1 cut(s) 25
HhaI GCGC 2 cut(s) 51, 221
Hin1I GRCGYC 2 cut(s) 17, 49
Hin1II CATG 6 cut(s) 42, 97, 205, 391, 435, 553
Hin6I GCGC 2 cut(s) 49, 219
HinP1I GCGC 2 cut(s) 49, 219
HincII GTYRAC 2 cut(s) 36, 465
HindII GTYRAC 2 cut(s) 36, 465
HinfI GANTC 3 cut(s) 100, 117, 177
HpaII CCGG 4 cut(s) 46, 90, 174, 222
HphI GGTGA 2 cut(s) 97, 346
Hpy166II GTNNAC 4 cut(s) 36, 127, 166, 465
Hpy188I TCNGA 4 cut(s) 12, 116, 718, 779
Hpy188III TCNNGA 6 cut(s) 128, 134, 202, 515, 619, 807
Hpy8I GTNNAC 4 cut(s) 36, 127, 166, 465
Hpy99I CGWCG 2 cut(s) 125, 152
HpyAV CCTTC 4 cut(s) 61, 199, 708, 813
HpyCH4III ACNGT 1 cut(s) 31
HpyCH4IV ACGT 1 cut(s) 591
HpyCH4V TGCA 7 cut(s) 166, 282, 410, 428, 553, 599, 611
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 158, 434, 443
HpyF3I CTNAG 2 cut(s) 156, 620
HpySE526I ACGT 1 cut(s) 591
Hsp92I GRCGYC 2 cut(s) 17, 49
Hsp92II CATG 6 cut(s) 42, 97, 205, 391, 435, 553
HspAI GCGC 2 cut(s) 49, 219
KasI GGCGCC 1 cut(s) 48
Kzo9I GATC 3 cut(s) 21, 130, 360
LmnI GCTCC 2 cut(s) 13, 117
Lsp1109I GCAGC 2 cut(s) 164, 796
MaeI CTAG 3 cut(s) 381, 443, 515
MaeII ACGT 1 cut(s) 591
MaeIII GTNAC 4 cut(s) 85, 334, 383, 752
MalI GATC 3 cut(s) 23, 132, 362
MboI GATC 3 cut(s) 21, 130, 360
MboII GAAGA 4 cut(s) 25, 136, 223, 785
MhlI GDGCHC 4 cut(s) 10, 168, 439, 631
Mly113I GGCGCC 1 cut(s) 49
MlyI GAGTC 1 cut(s) 94
MnlI CCTC 7 cut(s) 151, 152, 197, 353, 385, 537, 773
MseI TTAA 1 cut(s) 369
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 4 cut(s) 46, 90, 174, 222
MspR9I CCNGG 3 cut(s) 223, 496, 644
MvaI CCWGG 2 cut(s) 496, 644
MvnI CGCG 1 cut(s) 660
MwoI GCNNNNNNNGC 4 cut(s) 55, 158, 434, 443
NarI GGCGCC 1 cut(s) 49
NciI CCSGG 1 cut(s) 223
NdeII GATC 3 cut(s) 21, 130, 360
NlaIII CATG 6 cut(s) 42, 97, 205, 391, 435, 553
NlaIV GGNNCC 4 cut(s) 9, 44, 50, 617
NmuCI GTSAC 2 cut(s) 85, 334
PagI TCATGA 1 cut(s) 201
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PfeI GAWTC 2 cut(s) 117, 177
PflMI CCANNNNNTGG 1 cut(s) 345
PkrI GCNGC 2 cut(s) 154, 786
PleI GAGTC 1 cut(s) 94
PluTI GGCGCC 1 cut(s) 52
PpsI GAGTC 1 cut(s) 94
Ppu21I YACGTR 1 cut(s) 592
PshAI GACNNNNGTC 1 cut(s) 41
Psp6I CCWGG 2 cut(s) 494, 642
PspEI GGTNACC 2 cut(s) 85, 334
PspGI CCWGG 2 cut(s) 494, 642
PspN4I GGNNCC 4 cut(s) 9, 44, 50, 617
PspPI GGNCC 2 cut(s) 43, 498
PstI CTGCAG 1 cut(s) 284
PvuII CAGCTG 1 cut(s) 155
RsaI GTAC 2 cut(s) 278, 531
RsaNI GTAC 2 cut(s) 277, 530
SalI GTCGAC 1 cut(s) 34
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 2 cut(s) 153, 785
Sau3AI GATC 3 cut(s) 21, 130, 360
Sau96I GGNCC 2 cut(s) 43, 498
ScaI AGTACT 1 cut(s) 531
SchI GAGTC 1 cut(s) 94
ScrFI CCNGG 3 cut(s) 223, 496, 644
SduI GDGCHC 4 cut(s) 10, 168, 439, 631
SfcI CTRYAG 1 cut(s) 280
SfoI GGCGCC 1 cut(s) 50
SinI GGWCC 1 cut(s) 43
SsiI CCGC 4 cut(s) 56, 171, 364, 660
SspDI GGCGCC 1 cut(s) 48
SspMI CTAG 3 cut(s) 381, 443, 515
StyD4I CCNGG 3 cut(s) 221, 494, 642
StyI CCWWGG 1 cut(s) 678
TaaI ACNGT 1 cut(s) 31
TaiI ACGT 1 cut(s) 594
TaqI TCGA 1 cut(s) 35
TatI WGTACW 2 cut(s) 276, 529
TfiI GAWTC 2 cut(s) 117, 177
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 2 cut(s) 289, 336
TseFI GTSAC 2 cut(s) 85, 334
TseI GCWGC 2 cut(s) 152, 784
Tsp45I GTSAC 2 cut(s) 85, 334
TspDTI ATGAA 2 cut(s) 218, 724
TspGWI ACGGA 2 cut(s) 172, 450
TspRI CASTG 2 cut(s) 289, 336
Van91I CCANNNNNTGG 1 cut(s) 345
VneI GTGCAC 1 cut(s) 164
VpaK11BI GGWCC 1 cut(s) 43
XbaI TCTAGA 1 cut(s) 514
XmiI GTMKAC 1 cut(s) 35
XspI CTAG 3 cut(s) 381, 443, 515
ZrmI AGTACT 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.