Rh6BG027000

ER lumen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6B
OG0009847
Physical Location & Seq
Forward (+)
4087377 .. 4092125
4749 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6BG027000.1

Sequence Viewer

Length: 651 bp
ATGAGGGCTCCGAAGACGCCGATCAACACCGTGTCGACATGGGTCCGGCGCCAACCGCCGAAAGTGAAGGGCTTTCTGGCCGTGGTGACCGGCATGGCGACTCTGGTTCTGCTCCGATTCGTCGTTCACGATCACGATAACCTCTTCGTCGCAGCTGAGGCTGTGCACTCCGCCGGAATCTCCGTCCTCATCTATAAGCTCATGAAGGAGAAGACTTGCGCCGGGCTATCACTCAAATCGCAGGAACTTACAGCTATGTTTTTAGCTGTGAGATTGTACTGCAGTGTGGTTATGGAATATGACATACACACCCTGCTTGATTTGGCTACACTGGTGACCACCCTCTGGGTTATTTATATGATCCGCTTTAACTTGAAGTCTAGTTACATGGAGGACAAAGACAACTTTGCAATATACTATGTGGTTGCACCATGTGCTCTACTAGCTTTATTTATACATCCGTCAACTTCTCATCATTTATTGAACAGGATTTCCTGGGCTTTTTGTGTGTATCTAGAAGCTGTTTCAGTACTGCCCCAGTTGAGGGTCATGCAGAATACAAAGATTGTTGAGCCATTCACAGCTCATTATGTGTTTGCACTTGGTGTCGCAAGGTTCCTGAGCTGTGCCCATTGGGTTCTCCAGGTCTGA

Protein Analysis

216

Amino Acids

24.45

Weight (kDa)

9.11

Isoelectric Point (pI)

28.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ER_lumen_recept PF00810 72 - 214 7.8e-45 ER lumen protein retaining receptor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 48
AccB7I CCANNNNNTGG 1 cut(s) 345
AccI GTMKAC 1 cut(s) 35
AciI CCGC 3 cut(s) 56, 171, 364
AclWI GGATC 1 cut(s) 355
AcoI YGGCCR 1 cut(s) 78
AcyI GRCGYC 2 cut(s) 17, 49
AdeI CACNNNGTG 2 cut(s) 434, 605
AfaI GTAC 2 cut(s) 278, 531
AfiI CCNNNNNNNGG 3 cut(s) 345, 543, 544
AgsI TTSAA 2 cut(s) 376, 484
AjnI CCWGG 2 cut(s) 494, 642
AloI GAACNNNNNNTCC 2 cut(s) 476, 508
AluBI AGCT 8 cut(s) 155, 199, 254, 266, 446, 521, 584, 624
AluI AGCT 8 cut(s) 155, 199, 254, 266, 446, 521, 584, 624
Alw21I GWGCWC 2 cut(s) 168, 439
Alw44I GTGCAC 1 cut(s) 164
AlwI GGATC 1 cut(s) 355
AoxI GGCC 1 cut(s) 78
ApaLI GTGCAC 1 cut(s) 164
ApeKI GCWGC 1 cut(s) 152
AspLEI GCGC 2 cut(s) 51, 221
AspS9I GGNCC 1 cut(s) 43
AsuC2I CCSGG 1 cut(s) 223
AsuHPI GGTGA 2 cut(s) 97, 346
AvaII GGWCC 1 cut(s) 43
BaeGI GKGCMC 2 cut(s) 168, 631
BanI GGYRCC 1 cut(s) 48
BanII GRGCYC 1 cut(s) 10
BarI GAAGNNNNNNTAC 2 cut(s) 368, 400
BbsI GAAGAC 2 cut(s) 20, 218
Bbv12I GWGCWC 2 cut(s) 168, 439
BbvCI CCTCAGC 1 cut(s) 156
BbvI GCAGC 1 cut(s) 164
BceAI ACGGC 1 cut(s) 65
BciT130I CCWGG 2 cut(s) 496, 644
BcnI CCSGG 1 cut(s) 223
BfaI CTAG 3 cut(s) 381, 443, 515
BfmI CTRYAG 1 cut(s) 280
BfoI RGCGCY 1 cut(s) 52
BisI GCNGC 1 cut(s) 153
BlsI GCNGC 1 cut(s) 154
BmcAI AGTACT 1 cut(s) 531
Bme1390I CCNGG 3 cut(s) 223, 496, 644
Bme18I GGWCC 1 cut(s) 43
BmgT120I GGNCC 1 cut(s) 43
BmiI GGNNCC 4 cut(s) 9, 44, 50, 617
BmrFI CCNGG 3 cut(s) 223, 496, 644
BmrI ACTGGG 1 cut(s) 532
BmuI ACTGGG 1 cut(s) 532
BoxI GACNNNNGTC 1 cut(s) 41
BpiI GAAGAC 2 cut(s) 20, 218
BpmI CTGGAG 1 cut(s) 626
Bpu10I CCTNAGC 2 cut(s) 156, 620
BpuMI CCSGG 1 cut(s) 223
BsaHI GRCGYC 2 cut(s) 17, 49
BsaJI CCNNGG 2 cut(s) 81, 495
Bsc4I CCNNNNNNNGG 3 cut(s) 345, 543, 544
Bse118I RCCGGY 1 cut(s) 89
Bse1I ACTGG 2 cut(s) 336, 538
BseBI CCWGG 2 cut(s) 496, 644
BseDI CCNNGG 2 cut(s) 81, 495
BseGI GGATG 1 cut(s) 457
BseLI CCNNNNNNNGG 3 cut(s) 345, 543, 544
BseMII CTCAG 2 cut(s) 147, 611
BseNI ACTGG 2 cut(s) 336, 538
BseSI GKGCMC 2 cut(s) 168, 631
BseXI GCAGC 1 cut(s) 164
BshFI GGCC 1 cut(s) 80
BshNI GGYRCC 1 cut(s) 48
BsiHKAI GWGCWC 2 cut(s) 168, 439
BsiSI CCGG 4 cut(s) 46, 90, 174, 222
BslI CCNNNNNNNGG 3 cut(s) 345, 543, 544
BsnI GGCC 1 cut(s) 80
Bsp1286I GDGCHC 4 cut(s) 10, 168, 439, 631
Bsp143I GATC 3 cut(s) 21, 130, 360
BspACI CCGC 3 cut(s) 56, 171, 364
BspANI GGCC 1 cut(s) 80
BspCNI CTCAG 2 cut(s) 148, 612
BspHI TCATGA 1 cut(s) 201
BspLI GGNNCC 4 cut(s) 9, 44, 50, 617
BspMAI CTGCAG 1 cut(s) 284
BspPI GGATC 1 cut(s) 355
BspT107I GGYRCC 1 cut(s) 48
BsrFI RCCGGY 1 cut(s) 89
BsrI ACTGG 2 cut(s) 336, 538
BssAI RCCGGY 1 cut(s) 89
BssECI CCNNGG 2 cut(s) 81, 495
BssMI GATC 3 cut(s) 21, 130, 360
BssNI GRCGYC 2 cut(s) 17, 49
Bst2UI CCWGG 2 cut(s) 496, 644
Bst4CI ACNGT 1 cut(s) 31
Bst6I CTCTTC 1 cut(s) 149
BstACI GRCGYC 2 cut(s) 17, 49
BstAPI GCANNNNNTGC 1 cut(s) 434
BstDEI CTNAG 2 cut(s) 156, 620
BstDSI CCRYGG 1 cut(s) 81
BstEII GGTNACC 2 cut(s) 85, 334
BstF5I GGATG 1 cut(s) 457
BstH2I RGCGCY 1 cut(s) 52
BstHHI GCGC 2 cut(s) 51, 221
BstKTI GATC 3 cut(s) 24, 133, 363
BstMBI GATC 3 cut(s) 21, 130, 360
BstMWI GCNNNNNNNGC 4 cut(s) 55, 158, 434, 443
BstNI CCWGG 2 cut(s) 496, 644
BstPAI GACNNNNGTC 1 cut(s) 41
BstPI GGTNACC 2 cut(s) 85, 334
BstSCI CCNGG 3 cut(s) 221, 494, 642
BstSFI CTRYAG 1 cut(s) 280
BstSLI GKGCMC 2 cut(s) 168, 631
BstV1I GCAGC 1 cut(s) 164
BstV2I GAAGAC 2 cut(s) 20, 218
BsuRI GGCC 1 cut(s) 80
BtgI CCRYGG 1 cut(s) 81
BtsCI GGATG 1 cut(s) 457
BtsI GCAGTG 1 cut(s) 289
BtsIMutI CAGTG 2 cut(s) 289, 329
CciI TCATGA 1 cut(s) 201
CfoI GCGC 2 cut(s) 51, 221
Cfr10I RCCGGY 1 cut(s) 89
Cfr13I GGNCC 1 cut(s) 43
CseI GACGC 1 cut(s) 25
Csp6I GTAC 2 cut(s) 277, 530
CviAII CATG 6 cut(s) 39, 94, 202, 388, 432, 550
CviQI GTAC 2 cut(s) 277, 530
DdeI CTNAG 2 cut(s) 156, 620
DinI GGCGCC 1 cut(s) 50
DpnI GATC 3 cut(s) 23, 132, 362
DpnII GATC 3 cut(s) 21, 130, 360
DraIII CACNNNGTG 2 cut(s) 434, 605
EaeI YGGCCR 1 cut(s) 78
Eam1104I CTCTTC 1 cut(s) 149
EarI CTCTTC 1 cut(s) 149
EciI GGCGGA 1 cut(s) 160
Eco24I GRGCYC 1 cut(s) 10
Eco47I GGWCC 1 cut(s) 43
Eco91I GGTNACC 2 cut(s) 85, 334
EcoO65I GGTNACC 2 cut(s) 85, 334
EcoRII CCWGG 2 cut(s) 494, 642
EcoT38I GRGCYC 1 cut(s) 10
EgeI GGCGCC 1 cut(s) 50
EheI GGCGCC 1 cut(s) 50
FaeI CATG 6 cut(s) 42, 97, 205, 391, 435, 553
FatI CATG 6 cut(s) 38, 93, 201, 387, 431, 549
FblI GTMKAC 1 cut(s) 35
Fnu4HI GCNGC 1 cut(s) 153
FokI GGATG 1 cut(s) 444
FriOI GRGCYC 1 cut(s) 10
Fsp4HI GCNGC 1 cut(s) 153
FspBI CTAG 3 cut(s) 381, 443, 515
GlaI GCGC 2 cut(s) 50, 220
GluI GCNGC 1 cut(s) 153
GsuI CTGGAG 1 cut(s) 626
HaeII RGCGCY 1 cut(s) 52
HaeIII GGCC 1 cut(s) 80
HapII CCGG 4 cut(s) 46, 90, 174, 222
HgaI GACGC 1 cut(s) 25
HhaI GCGC 2 cut(s) 51, 221
Hin1I GRCGYC 2 cut(s) 17, 49
Hin1II CATG 6 cut(s) 42, 97, 205, 391, 435, 553
Hin6I GCGC 2 cut(s) 49, 219
HinP1I GCGC 2 cut(s) 49, 219
HincII GTYRAC 2 cut(s) 36, 465
HindII GTYRAC 2 cut(s) 36, 465
HinfI GANTC 3 cut(s) 100, 117, 177
HpaII CCGG 4 cut(s) 46, 90, 174, 222
HphI GGTGA 2 cut(s) 97, 346
Hpy166II GTNNAC 4 cut(s) 36, 127, 166, 465
Hpy188I TCNGA 3 cut(s) 12, 116, 650
Hpy188III TCNNGA 5 cut(s) 128, 134, 202, 515, 619
Hpy8I GTNNAC 4 cut(s) 36, 127, 166, 465
Hpy99I CGWCG 2 cut(s) 125, 152
HpyAV CCTTC 2 cut(s) 61, 199
HpyCH4III ACNGT 1 cut(s) 31
HpyCH4V TGCA 6 cut(s) 166, 282, 410, 428, 553, 599
HpyF10VI GCNNNNNNNGC 4 cut(s) 55, 158, 434, 443
HpyF3I CTNAG 2 cut(s) 156, 620
Hsp92I GRCGYC 2 cut(s) 17, 49
Hsp92II CATG 6 cut(s) 42, 97, 205, 391, 435, 553
HspAI GCGC 2 cut(s) 49, 219
KasI GGCGCC 1 cut(s) 48
Kzo9I GATC 3 cut(s) 21, 130, 360
LmnI GCTCC 2 cut(s) 13, 117
Lsp1109I GCAGC 1 cut(s) 164
MaeI CTAG 3 cut(s) 381, 443, 515
MaeIII GTNAC 3 cut(s) 85, 334, 383
MalI GATC 3 cut(s) 23, 132, 362
MboI GATC 3 cut(s) 21, 130, 360
MboII GAAGA 3 cut(s) 25, 136, 223
MhlI GDGCHC 4 cut(s) 10, 168, 439, 631
Mly113I GGCGCC 1 cut(s) 49
MlyI GAGTC 1 cut(s) 94
MnlI CCTC 6 cut(s) 151, 152, 197, 353, 385, 537
MseI TTAA 1 cut(s) 369
MspA1I CMGCKG 1 cut(s) 155
MspI CCGG 4 cut(s) 46, 90, 174, 222
MspR9I CCNGG 3 cut(s) 223, 496, 644
MvaI CCWGG 2 cut(s) 496, 644
MwoI GCNNNNNNNGC 4 cut(s) 55, 158, 434, 443
NarI GGCGCC 1 cut(s) 49
NciI CCSGG 1 cut(s) 223
NdeII GATC 3 cut(s) 21, 130, 360
NlaIII CATG 6 cut(s) 42, 97, 205, 391, 435, 553
NlaIV GGNNCC 4 cut(s) 9, 44, 50, 617
NmuCI GTSAC 2 cut(s) 85, 334
PagI TCATGA 1 cut(s) 201
PcsI WCGNNNNNNNCGW 1 cut(s) 126
PfeI GAWTC 2 cut(s) 117, 177
PflMI CCANNNNNTGG 1 cut(s) 345
PkrI GCNGC 1 cut(s) 154
PleI GAGTC 1 cut(s) 94
PluTI GGCGCC 1 cut(s) 52
PpsI GAGTC 1 cut(s) 94
PshAI GACNNNNGTC 1 cut(s) 41
Psp6I CCWGG 2 cut(s) 494, 642
PspEI GGTNACC 2 cut(s) 85, 334
PspGI CCWGG 2 cut(s) 494, 642
PspN4I GGNNCC 4 cut(s) 9, 44, 50, 617
PspPI GGNCC 1 cut(s) 43
PstI CTGCAG 1 cut(s) 284
PvuII CAGCTG 1 cut(s) 155
RsaI GTAC 2 cut(s) 278, 531
RsaNI GTAC 2 cut(s) 277, 530
SalI GTCGAC 1 cut(s) 34
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 1 cut(s) 153
Sau3AI GATC 3 cut(s) 21, 130, 360
Sau96I GGNCC 1 cut(s) 43
ScaI AGTACT 1 cut(s) 531
SchI GAGTC 1 cut(s) 94
ScrFI CCNGG 3 cut(s) 223, 496, 644
SduI GDGCHC 4 cut(s) 10, 168, 439, 631
SfcI CTRYAG 1 cut(s) 280
SfoI GGCGCC 1 cut(s) 50
SinI GGWCC 1 cut(s) 43
SsiI CCGC 3 cut(s) 56, 171, 364
SspDI GGCGCC 1 cut(s) 48
SspMI CTAG 3 cut(s) 381, 443, 515
StyD4I CCNGG 3 cut(s) 221, 494, 642
TaaI ACNGT 1 cut(s) 31
TaqI TCGA 1 cut(s) 35
TatI WGTACW 2 cut(s) 276, 529
TfiI GAWTC 2 cut(s) 117, 177
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 2 cut(s) 289, 336
TseFI GTSAC 2 cut(s) 85, 334
TseI GCWGC 1 cut(s) 152
Tsp45I GTSAC 2 cut(s) 85, 334
TspDTI ATGAA 1 cut(s) 218
TspGWI ACGGA 2 cut(s) 172, 450
TspRI CASTG 2 cut(s) 289, 336
Van91I CCANNNNNTGG 1 cut(s) 345
VneI GTGCAC 1 cut(s) 164
VpaK11BI GGWCC 1 cut(s) 43
XbaI TCTAGA 1 cut(s) 514
XmiI GTMKAC 1 cut(s) 35
XspI CTAG 3 cut(s) 381, 443, 515
ZrmI AGTACT 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.