MD10G1116900.v1.1

ER lumen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009847
Physical Location & Seq
Reverse (-)
19455578 .. 19458415
2838 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1116900.v1.1.491

Sequence Viewer

Length: 819 bp
ATGAGGGCTCCGAGGAGGCCGATCCACACCATATGGACATGGGCGCGACGCCAGCCGTCCAAAGTTCAGGCCTTTCTGGCCGTCGTTTCGGGCATGGCGGCGCTGGTTCTGCTCCGATTCATCGTCCACGATCATGACAATCTCTTCGTTGCAGCTGAGGCTGTGCACTCGATTGGAATCTCCGTTCTTATCTACAAGCTTATGAAGGAGAAGACTTGCGCTGGCCTATCACTCAAAACGCAGGAATTAACAGCTATGTTTTTAGCTGTGAGATTATATTGTAGTGTTGTTATGGAATATGATATACACACCCTGCTAGATTTAGCTACCCTGGTGACGACCCTCTGGGTTATTTATATGATCCGGTTCAACTTGAAGTCTAGTTACATGGAGGATAAAGACAACTTCGCAATATATTATGTCGTGGTACCATGTGCTCTGTTAGCCTTGTTTATTCATCCATCAACTTCTCATCATTTGCTGAACAGGATTTCCTGGGCATTTTGTGTGTATCTGGAAGCCGTTTCTGTACTGCCCCAGTTGCGGGTCATGCAAAACACAAAGATTGTTGAGCCATTCACAGCTCATTATGTGTTTGCACTAGGTGTCGCCAGGTTCCTCAGCTGTGCCCATTGGGTTCTCCAGGTATTAGATACTCGCGGACACTTGCTTGTAGCCTTGGGGTATGGGTTATGGCCATCCATGGTTCTTATTTCAGAAATTGTCCAGACTTTCATATTAGCAGACTTCTGTTACTACTACATCAAAAGTGTTTTCGGAGGGCAGCTTGTCCTCCGTCTCCCTTCCGGAGTTGTATGA

Protein Analysis

273

Amino Acids

30.91

Weight (kDa)

9.0

Isoelectric Point (pI)

31.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ER_lumen_recept PF00810 72 - 214 4.4e-45 ER lumen protein retaining receptor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 427
AccB1I GGYRCC 1 cut(s) 427
AccII CGCG 2 cut(s) 46, 660
AccIII TCCGGA 1 cut(s) 806
AciI CCGC 3 cut(s) 98, 544, 660
AclWI GGATC 2 cut(s) 16, 355
AcoI YGGCCR 2 cut(s) 78, 695
AcyI GRCGYC 1 cut(s) 49
AdeI CACNNNGTG 1 cut(s) 605
AfaI GTAC 2 cut(s) 429, 531
AfiI CCNNNNNNNGG 2 cut(s) 543, 544
AgsI TTSAA 2 cut(s) 370, 376
AjnI CCWGG 4 cut(s) 330, 494, 611, 642
AloI GAACNNNNNNTCC 4 cut(s) 168, 200, 476, 508
AluBI AGCT 8 cut(s) 155, 199, 254, 266, 326, 584, 624, 787
AluI AGCT 8 cut(s) 155, 199, 254, 266, 326, 584, 624, 787
Alw21I GWGCWC 2 cut(s) 168, 439
Alw26I GTCTC 1 cut(s) 803
Alw44I GTGCAC 1 cut(s) 164
AlwI GGATC 2 cut(s) 16, 355
Aor13HI TCCGGA 1 cut(s) 806
AoxI GGCC 5 cut(s) 17, 69, 78, 223, 695
ApaLI GTGCAC 1 cut(s) 164
ApeKI GCWGC 2 cut(s) 152, 784
ArsI GACNNNNNNTTYG 2 cut(s) 128, 160
Asp718I GGTACC 1 cut(s) 427
AspLEI GCGC 3 cut(s) 46, 103, 221
AsuHPI GGTGA 1 cut(s) 346
BaeGI GKGCMC 2 cut(s) 168, 631
BalI TGGCCA 1 cut(s) 697
BanI GGYRCC 1 cut(s) 427
BanII GRGCYC 1 cut(s) 10
BarI GAAGNNNNNNTAC 2 cut(s) 368, 400
BbsI GAAGAC 1 cut(s) 218
Bbv12I GWGCWC 2 cut(s) 168, 439
BbvCI CCTCAGC 2 cut(s) 156, 620
BbvI GCAGC 2 cut(s) 164, 796
BccI CCATC 2 cut(s) 469, 706
BceAI ACGGC 3 cut(s) 40, 65, 506
BciT130I CCWGG 4 cut(s) 332, 496, 613, 644
BcoDI GTCTC 1 cut(s) 803
BfaI CTAG 3 cut(s) 317, 381, 602
BfoI RGCGCY 1 cut(s) 104
BglI GCCNNNNNGGC 1 cut(s) 77
BisI GCNGC 3 cut(s) 99, 153, 785
BlsI GCNGC 3 cut(s) 100, 154, 786
Bme1390I CCNGG 4 cut(s) 332, 496, 613, 644
BmiI GGNNCC 3 cut(s) 9, 429, 617
BmrFI CCNGG 4 cut(s) 332, 496, 613, 644
BmrI ACTGGG 1 cut(s) 532
BmuI ACTGGG 1 cut(s) 532
BpiI GAAGAC 1 cut(s) 218
BpmI CTGGAG 1 cut(s) 626
Bpu10I CCTNAGC 2 cut(s) 156, 620
BsaBI GATNNNNATC 1 cut(s) 176
BsaHI GRCGYC 1 cut(s) 49
BsaJI CCNNGG 5 cut(s) 11, 330, 495, 678, 702
BsaWI WCCGGW 2 cut(s) 363, 806
Bsc4I CCNNNNNNNGG 2 cut(s) 543, 544
Bse1I ACTGG 1 cut(s) 538
Bse8I GATNNNNATC 1 cut(s) 176
BseAI TCCGGA 1 cut(s) 806
BseBI CCWGG 4 cut(s) 332, 496, 613, 644
BseDI CCNNGG 5 cut(s) 11, 330, 495, 678, 702
BseGI GGATG 2 cut(s) 457, 698
BseJI GATNNNNATC 1 cut(s) 176
BseLI CCNNNNNNNGG 2 cut(s) 543, 544
BseMII CTCAG 2 cut(s) 147, 634
BseNI ACTGG 1 cut(s) 538
BseRI GAGGAG 1 cut(s) 28
BseSI GKGCMC 2 cut(s) 168, 631
BseXI GCAGC 2 cut(s) 164, 796
Bsh1236I CGCG 2 cut(s) 46, 660
BshFI GGCC 5 cut(s) 19, 71, 80, 225, 697
BshNI GGYRCC 1 cut(s) 427
BsiHKAI GWGCWC 2 cut(s) 168, 439
BsiSI CCGG 2 cut(s) 364, 807
BslI CCNNNNNNNGG 2 cut(s) 543, 544
BsmAI GTCTC 1 cut(s) 803
BsmBI CGTCTC 1 cut(s) 803
BsnI GGCC 5 cut(s) 19, 71, 80, 225, 697
Bsp1286I GDGCHC 4 cut(s) 10, 168, 439, 631
Bsp13I TCCGGA 1 cut(s) 806
Bsp143I GATC 3 cut(s) 21, 130, 360
Bsp19I CCATGG 1 cut(s) 702
BspACI CCGC 3 cut(s) 98, 544, 660
BspANI GGCC 5 cut(s) 19, 71, 80, 225, 697
BspCNI CTCAG 2 cut(s) 148, 633
BspEI TCCGGA 1 cut(s) 806
BspFNI CGCG 2 cut(s) 46, 660
BspHI TCATGA 1 cut(s) 133
BspLI GGNNCC 3 cut(s) 9, 429, 617
BspPI GGATC 2 cut(s) 16, 355
BspT107I GGYRCC 1 cut(s) 427
BsrI ACTGG 1 cut(s) 538
BssECI CCNNGG 5 cut(s) 11, 330, 495, 678, 702
BssMI GATC 3 cut(s) 21, 130, 360
BssNI GRCGYC 1 cut(s) 49
BssT1I CCWWGG 2 cut(s) 678, 702
Bst2UI CCWGG 4 cut(s) 332, 496, 613, 644
Bst6I CTCTTC 1 cut(s) 149
BstACI GRCGYC 1 cut(s) 49
BstC8I GCNNGC 2 cut(s) 53, 223
BstDEI CTNAG 2 cut(s) 156, 620
BstDSI CCRYGG 1 cut(s) 702
BstF5I GGATG 2 cut(s) 457, 698
BstFNI CGCG 2 cut(s) 46, 660
BstH2I RGCGCY 1 cut(s) 104
BstHHI GCGC 3 cut(s) 46, 103, 221
BstKTI GATC 3 cut(s) 24, 133, 363
BstMAI GTCTC 1 cut(s) 803
BstMBI GATC 3 cut(s) 21, 130, 360
BstMWI GCNNNNNNNGC 7 cut(s) 52, 77, 109, 158, 443, 541, 550
BstNI CCWGG 4 cut(s) 332, 496, 613, 644
BstSCI CCNGG 4 cut(s) 330, 494, 611, 642
BstSLI GKGCMC 2 cut(s) 168, 631
BstUI CGCG 2 cut(s) 46, 660
BstV1I GCAGC 2 cut(s) 164, 796
BstV2I GAAGAC 1 cut(s) 218
BsuRI GGCC 5 cut(s) 19, 71, 80, 225, 697
BtgI CCRYGG 1 cut(s) 702
BtsCI GGATG 2 cut(s) 457, 698
Cac8I GCNNGC 2 cut(s) 53, 223
CciI TCATGA 1 cut(s) 133
CfoI GCGC 3 cut(s) 46, 103, 221
CseI GACGC 1 cut(s) 57
Csp6I GTAC 2 cut(s) 428, 530
CviAII CATG 7 cut(s) 39, 94, 134, 388, 432, 550, 703
CviQI GTAC 2 cut(s) 428, 530
DdeI CTNAG 2 cut(s) 156, 620
DpnI GATC 3 cut(s) 23, 132, 362
DpnII GATC 3 cut(s) 21, 130, 360
DraIII CACNNNGTG 1 cut(s) 605
EaeI YGGCCR 2 cut(s) 78, 695
Eam1104I CTCTTC 1 cut(s) 149
EarI CTCTTC 1 cut(s) 149
Eco130I CCWWGG 2 cut(s) 678, 702
Eco147I AGGCCT 1 cut(s) 71
Eco24I GRGCYC 1 cut(s) 10
EcoRII CCWGG 4 cut(s) 330, 494, 611, 642
EcoT14I CCWWGG 2 cut(s) 678, 702
EcoT38I GRGCYC 1 cut(s) 10
ErhI CCWWGG 2 cut(s) 678, 702
Esp3I CGTCTC 1 cut(s) 803
FaeI CATG 7 cut(s) 42, 97, 137, 391, 435, 553, 706
FatI CATG 7 cut(s) 38, 93, 133, 387, 431, 549, 702
FauI CCCGC 1 cut(s) 537
FauNDI CATATG 1 cut(s) 32
Fnu4HI GCNGC 3 cut(s) 99, 153, 785
FokI GGATG 2 cut(s) 444, 685
FriOI GRGCYC 1 cut(s) 10
Fsp4HI GCNGC 3 cut(s) 99, 153, 785
FspBI CTAG 3 cut(s) 317, 381, 602
GlaI GCGC 3 cut(s) 45, 102, 220
GluI GCNGC 3 cut(s) 99, 153, 785
GsuI CTGGAG 1 cut(s) 626
HaeII RGCGCY 1 cut(s) 104
HaeIII GGCC 5 cut(s) 19, 71, 80, 225, 697
HapII CCGG 2 cut(s) 364, 807
HgaI GACGC 1 cut(s) 57
HhaI GCGC 3 cut(s) 46, 103, 221
Hin1I GRCGYC 1 cut(s) 49
Hin1II CATG 7 cut(s) 42, 97, 137, 391, 435, 553, 706
Hin6I GCGC 3 cut(s) 44, 101, 219
HinP1I GCGC 3 cut(s) 44, 101, 219
HindIII AAGCTT 1 cut(s) 197
HinfI GANTC 2 cut(s) 117, 177
HpaII CCGG 2 cut(s) 364, 807
HphI GGTGA 1 cut(s) 346
Hpy166II GTNNAC 2 cut(s) 127, 166
Hpy188I TCNGA 4 cut(s) 12, 116, 718, 779
Hpy188III TCNNGA 4 cut(s) 134, 515, 727, 807
Hpy8I GTNNAC 2 cut(s) 127, 166
Hpy99I CGWCG 2 cut(s) 51, 86
HpyAV CCTTC 2 cut(s) 199, 813
HpyCH4V TGCA 4 cut(s) 152, 166, 553, 599
HpyF10VI GCNNNNNNNGC 7 cut(s) 52, 77, 109, 158, 443, 541, 550
HpyF3I CTNAG 2 cut(s) 156, 620
Hsp92I GRCGYC 1 cut(s) 49
Hsp92II CATG 7 cut(s) 42, 97, 137, 391, 435, 553, 706
HspAI GCGC 3 cut(s) 44, 101, 219
Kpn2I TCCGGA 1 cut(s) 806
KpnI GGTACC 1 cut(s) 431
Kzo9I GATC 3 cut(s) 21, 130, 360
LmnI GCTCC 2 cut(s) 13, 117
Lsp1109I GCAGC 2 cut(s) 164, 796
MaeI CTAG 3 cut(s) 317, 381, 602
MaeIII GTNAC 3 cut(s) 334, 383, 752
MalI GATC 3 cut(s) 23, 132, 362
MboI GATC 3 cut(s) 21, 130, 360
MboII GAAGA 2 cut(s) 136, 223
MhlI GDGCHC 4 cut(s) 10, 168, 439, 631
MlsI TGGCCA 1 cut(s) 697
MluCI AATT 2 cut(s) 245, 720
MluNI TGGCCA 1 cut(s) 697
MnlI CCTC 8 cut(s) 6, 9, 151, 353, 385, 629, 773, 803
Mox20I TGGCCA 1 cut(s) 697
MroI TCCGGA 1 cut(s) 806
MscI TGGCCA 1 cut(s) 697
MseI TTAA 1 cut(s) 248
MslI CAYNNNNRTG 1 cut(s) 132
Msp20I TGGCCA 1 cut(s) 697
MspA1I CMGCKG 2 cut(s) 155, 624
MspI CCGG 2 cut(s) 364, 807
MspR9I CCNGG 4 cut(s) 332, 496, 613, 644
MvaI CCWGG 4 cut(s) 332, 496, 613, 644
MvnI CGCG 2 cut(s) 46, 660
MwoI GCNNNNNNNGC 7 cut(s) 52, 77, 109, 158, 443, 541, 550
NcoI CCATGG 1 cut(s) 702
NdeI CATATG 1 cut(s) 32
NdeII GATC 3 cut(s) 21, 130, 360
NlaIII CATG 7 cut(s) 42, 97, 137, 391, 435, 553, 706
NlaIV GGNNCC 3 cut(s) 9, 429, 617
NmuCI GTSAC 1 cut(s) 334
PagI TCATGA 1 cut(s) 133
PceI AGGCCT 1 cut(s) 71
PfeI GAWTC 2 cut(s) 117, 177
PkrI GCNGC 3 cut(s) 100, 154, 786
Psp6I CCWGG 4 cut(s) 330, 494, 611, 642
PspGI CCWGG 4 cut(s) 330, 494, 611, 642
PspN4I GGNNCC 3 cut(s) 9, 429, 617
PvuII CAGCTG 2 cut(s) 155, 624
RsaI GTAC 2 cut(s) 429, 531
RsaNI GTAC 2 cut(s) 428, 530
RseI CAYNNNNRTG 1 cut(s) 132
SaqAI TTAA 1 cut(s) 248
SatI GCNGC 3 cut(s) 99, 153, 785
Sau3AI GATC 3 cut(s) 21, 130, 360
ScrFI CCNGG 4 cut(s) 332, 496, 613, 644
SduI GDGCHC 4 cut(s) 10, 168, 439, 631
SfiI GGCCNNNNNGGCC 1 cut(s) 77
SmiMI CAYNNNNRTG 1 cut(s) 132
Sse9I AATT 2 cut(s) 245, 720
SseBI AGGCCT 1 cut(s) 71
SsiI CCGC 3 cut(s) 98, 544, 660
SspMI CTAG 3 cut(s) 317, 381, 602
StuI AGGCCT 1 cut(s) 71
StyD4I CCNGG 4 cut(s) 330, 494, 611, 642
StyI CCWWGG 2 cut(s) 678, 702
TaqI TCGA 1 cut(s) 170
TasI AATT 2 cut(s) 245, 720
TatI WGTACW 1 cut(s) 529
TauI GCSGC 1 cut(s) 101
TfiI GAWTC 2 cut(s) 117, 177
Tru1I TTAA 1 cut(s) 248
Tru9I TTAA 1 cut(s) 248
TseFI GTSAC 1 cut(s) 334
TseI GCWGC 2 cut(s) 152, 784
Tsp45I GTSAC 1 cut(s) 334
TspDTI ATGAA 4 cut(s) 109, 218, 446, 724
TspGWI ACGGA 2 cut(s) 172, 785
VneI GTGCAC 1 cut(s) 164
XspI CTAG 3 cut(s) 317, 381, 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.