RLG00000015300

ER lumen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr3
OG0009847
Physical Location & Seq
Reverse (-)
64193986 .. 64196256
2271 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000015300

Sequence Viewer

Length: 726 bp
ATGGCGACTCTGGTTCTGCTCCGATTCGTCGTTCACGATCACGATAACCTCTTCGTCGCAGCTGAGGCTGTGCACTCCGCCGGAATCTCCGTCCTCATCTATAAGCTCATGAAGGAGAAGACTTGCGCCGGGCTATCACTCAAATCGCAGGAACTTACAGCTATGTTTTTAGCTGTGAGATTGTACTGCAGTGTGGTTATGGAATATGACATACACACCCTGCTTGATTTAGCTACACTGGTGACCACCCTCTGGGTTATTTATATGATCCGCTTCAACTTGAAGTCTAGTTACATGGAGGACAAAGACAACTTTGCAATATACTATGTGGTTGCACCGTGTGCTCTACTAGCTTTATTTATACATCCGTCAACTTCTCATCATTTATTGAACAGGATTTCCTGGGCCTTTTGTGTGTATCTAGAAGCTGTTTCAGTACTGCCTCAGTTGAGGGTCATGCAGAATACAAAGATTGTTGAGCCATTCACAGCTCATTACGTGTTTGCACTTGGTGTCGCAAGGTTCCTGAGCTGTGCCCATTGGGTTCTCCAGGTATTAGATACTCGCGGACATTTGCTGGTGGCCTTGGGGTATGGGTTGTGGCCTTCTATGGTTCTTATTTCGGAAATTGTCCAAACTTTTATATTAGCAGACTTCTGTTACTACTATGTCAAAAGTGTTTTCGGAGGGCAGCTTGTTCTTCGTCTCCCTTCTGGAGTTGTATGA

Protein Analysis

242

Amino Acids

27.23

Weight (kDa)

7.13

Isoelectric Point (pI)

29.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ER_lumen_recept PF00810 41 - 183 1.1e-44 ER lumen protein retaining receptor
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 252
AccII CGCG 1 cut(s) 567
AciI CCGC 3 cut(s) 78, 271, 567
AclWI GGATC 1 cut(s) 262
AdeI CACNNNGTG 2 cut(s) 341, 512
AfaI GTAC 2 cut(s) 185, 438
AfiI CCNNNNNNNGG 1 cut(s) 252
AflIII ACRYGT 1 cut(s) 498
AgsI TTSAA 3 cut(s) 277, 283, 391
AjnI CCWGG 2 cut(s) 401, 549
AloI GAACNNNNNNTCC 2 cut(s) 383, 415
Alw21I GWGCWC 2 cut(s) 75, 346
Alw26I GTCTC 1 cut(s) 710
Alw44I GTGCAC 1 cut(s) 71
AlwI GGATC 1 cut(s) 262
AoxI GGCC 3 cut(s) 405, 582, 602
ApaLI GTGCAC 1 cut(s) 71
ApeKI GCWGC 2 cut(s) 59, 691
AspLEI GCGC 1 cut(s) 128
AspS9I GGNCC 1 cut(s) 405
AsuC2I CCSGG 1 cut(s) 130
AsuHPI GGTGA 1 cut(s) 253
BaeGI GKGCMC 2 cut(s) 75, 538
BarI GAAGNNNNNNTAC 2 cut(s) 275, 307
BbsI GAAGAC 1 cut(s) 125
Bbv12I GWGCWC 2 cut(s) 75, 346
BbvCI CCTCAGC 1 cut(s) 63
BbvI GCAGC 2 cut(s) 71, 703
BciT130I CCWGG 2 cut(s) 403, 551
BcnI CCSGG 1 cut(s) 130
BcoDI GTCTC 1 cut(s) 710
BfaI CTAG 3 cut(s) 288, 350, 422
BfmI CTRYAG 1 cut(s) 187
BisI GCNGC 2 cut(s) 60, 692
BlsI GCNGC 2 cut(s) 61, 693
BmcAI AGTACT 1 cut(s) 438
Bme1390I CCNGG 3 cut(s) 130, 403, 551
BmgT120I GGNCC 1 cut(s) 405
BmiI GGNNCC 1 cut(s) 524
BmrFI CCNGG 3 cut(s) 130, 403, 551
BpiI GAAGAC 1 cut(s) 125
BpmI CTGGAG 1 cut(s) 533
Bpu10I CCTNAGC 2 cut(s) 63, 527
BpuMI CCSGG 1 cut(s) 130
BsaAI YACGTR 1 cut(s) 499
BsaJI CCNNGG 2 cut(s) 402, 585
Bsc4I CCNNNNNNNGG 1 cut(s) 252
Bse1I ACTGG 1 cut(s) 243
BseBI CCWGG 2 cut(s) 403, 551
BseDI CCNNGG 2 cut(s) 402, 585
BseGI GGATG 1 cut(s) 364
BseLI CCNNNNNNNGG 1 cut(s) 252
BseMII CTCAG 3 cut(s) 54, 458, 518
BseNI ACTGG 1 cut(s) 243
BseSI GKGCMC 2 cut(s) 75, 538
BseXI GCAGC 2 cut(s) 71, 703
Bsh1236I CGCG 1 cut(s) 567
BshFI GGCC 3 cut(s) 407, 584, 604
BsiHKAI GWGCWC 2 cut(s) 75, 346
BsiSI CCGG 2 cut(s) 81, 129
BslI CCNNNNNNNGG 1 cut(s) 252
BsmAI GTCTC 1 cut(s) 710
BsmBI CGTCTC 1 cut(s) 710
BsnI GGCC 3 cut(s) 407, 584, 604
Bsp1286I GDGCHC 3 cut(s) 75, 346, 538
Bsp143I GATC 2 cut(s) 37, 267
BspACI CCGC 3 cut(s) 78, 271, 567
BspANI GGCC 3 cut(s) 407, 584, 604
BspCNI CTCAG 3 cut(s) 55, 457, 519
BspFNI CGCG 1 cut(s) 567
BspHI TCATGA 1 cut(s) 108
BspLI GGNNCC 1 cut(s) 524
BspMAI CTGCAG 1 cut(s) 191
BspPI GGATC 1 cut(s) 262
BsrI ACTGG 1 cut(s) 243
BssECI CCNNGG 2 cut(s) 402, 585
BssMI GATC 2 cut(s) 37, 267
BssT1I CCWWGG 1 cut(s) 585
Bst2UI CCWGG 2 cut(s) 403, 551
Bst4CI ACNGT 1 cut(s) 339
Bst6I CTCTTC 1 cut(s) 56
BstAPI GCANNNNNTGC 1 cut(s) 341
BstBAI YACGTR 1 cut(s) 499
BstDEI CTNAG 3 cut(s) 63, 444, 527
BstEII GGTNACC 1 cut(s) 241
BstF5I GGATG 1 cut(s) 364
BstFNI CGCG 1 cut(s) 567
BstHHI GCGC 1 cut(s) 128
BstKTI GATC 2 cut(s) 40, 270
BstMAI GTCTC 1 cut(s) 710
BstMBI GATC 2 cut(s) 37, 267
BstMWI GCNNNNNNNGC 3 cut(s) 65, 341, 350
BstNI CCWGG 2 cut(s) 403, 551
BstPI GGTNACC 1 cut(s) 241
BstSCI CCNGG 3 cut(s) 128, 401, 549
BstSFI CTRYAG 1 cut(s) 187
BstSLI GKGCMC 2 cut(s) 75, 538
BstUI CGCG 1 cut(s) 567
BstV1I GCAGC 2 cut(s) 71, 703
BstV2I GAAGAC 1 cut(s) 125
BsuRI GGCC 3 cut(s) 407, 584, 604
BtsCI GGATG 1 cut(s) 364
BtsI GCAGTG 1 cut(s) 196
BtsIMutI CAGTG 2 cut(s) 196, 236
CciI TCATGA 1 cut(s) 108
CfoI GCGC 1 cut(s) 128
Cfr13I GGNCC 1 cut(s) 405
Csp6I GTAC 2 cut(s) 184, 437
CviAII CATG 3 cut(s) 109, 295, 457
CviQI GTAC 2 cut(s) 184, 437
DdeI CTNAG 3 cut(s) 63, 444, 527
DpnI GATC 2 cut(s) 39, 269
DpnII GATC 2 cut(s) 37, 267
DraIII CACNNNGTG 2 cut(s) 341, 512
Eam1104I CTCTTC 1 cut(s) 56
EarI CTCTTC 1 cut(s) 56
EciI GGCGGA 1 cut(s) 67
Eco130I CCWWGG 1 cut(s) 585
Eco91I GGTNACC 1 cut(s) 241
EcoO65I GGTNACC 1 cut(s) 241
EcoRII CCWGG 2 cut(s) 401, 549
EcoT14I CCWWGG 1 cut(s) 585
ErhI CCWWGG 1 cut(s) 585
Esp3I CGTCTC 1 cut(s) 710
FaeI CATG 3 cut(s) 112, 298, 460
FatI CATG 3 cut(s) 108, 294, 456
Fnu4HI GCNGC 2 cut(s) 60, 692
FokI GGATG 1 cut(s) 351
Fsp4HI GCNGC 2 cut(s) 60, 692
FspBI CTAG 3 cut(s) 288, 350, 422
GlaI GCGC 1 cut(s) 127
GluI GCNGC 2 cut(s) 60, 692
GsuI CTGGAG 1 cut(s) 533
HaeIII GGCC 3 cut(s) 407, 584, 604
HapII CCGG 2 cut(s) 81, 129
HhaI GCGC 1 cut(s) 128
Hin1II CATG 3 cut(s) 112, 298, 460
Hin6I GCGC 1 cut(s) 126
HinP1I GCGC 1 cut(s) 126
HincII GTYRAC 1 cut(s) 372
HindII GTYRAC 1 cut(s) 372
HinfI GANTC 3 cut(s) 7, 24, 84
HpaII CCGG 2 cut(s) 81, 129
HphI GGTGA 1 cut(s) 253
Hpy166II GTNNAC 3 cut(s) 34, 73, 372
Hpy188I TCNGA 3 cut(s) 23, 625, 686
Hpy188III TCNNGA 6 cut(s) 35, 41, 109, 422, 526, 714
Hpy8I GTNNAC 3 cut(s) 34, 73, 372
Hpy99I CGWCG 2 cut(s) 32, 59
HpyAV CCTTC 3 cut(s) 106, 615, 720
HpyCH4III ACNGT 1 cut(s) 339
HpyCH4IV ACGT 1 cut(s) 498
HpyCH4V TGCA 6 cut(s) 73, 189, 317, 335, 460, 506
HpyF10VI GCNNNNNNNGC 3 cut(s) 65, 341, 350
HpyF3I CTNAG 3 cut(s) 63, 444, 527
HpySE526I ACGT 1 cut(s) 498
Hsp92II CATG 3 cut(s) 112, 298, 460
HspAI GCGC 1 cut(s) 126
Kzo9I GATC 2 cut(s) 37, 267
LmnI GCTCC 1 cut(s) 24
Lsp1109I GCAGC 2 cut(s) 71, 703
MaeI CTAG 3 cut(s) 288, 350, 422
MaeII ACGT 1 cut(s) 498
MaeIII GTNAC 3 cut(s) 241, 290, 659
MalI GATC 2 cut(s) 39, 269
MboI GATC 2 cut(s) 37, 267
MboII GAAGA 3 cut(s) 43, 130, 692
MhlI GDGCHC 3 cut(s) 75, 346, 538
MluCI AATT 1 cut(s) 627
MnlI CCTC 8 cut(s) 58, 59, 104, 260, 292, 444, 453, 680
MspA1I CMGCKG 1 cut(s) 62
MspI CCGG 2 cut(s) 81, 129
MspR9I CCNGG 3 cut(s) 130, 403, 551
MvaI CCWGG 2 cut(s) 403, 551
MvnI CGCG 1 cut(s) 567
MwoI GCNNNNNNNGC 3 cut(s) 65, 341, 350
NciI CCSGG 1 cut(s) 130
NdeII GATC 2 cut(s) 37, 267
NlaIII CATG 3 cut(s) 112, 298, 460
NlaIV GGNNCC 1 cut(s) 524
NmuCI GTSAC 1 cut(s) 241
PagI TCATGA 1 cut(s) 108
PcsI WCGNNNNNNNCGW 1 cut(s) 33
PfeI GAWTC 2 cut(s) 24, 84
PflMI CCANNNNNTGG 1 cut(s) 252
PkrI GCNGC 2 cut(s) 61, 693
Ppu21I YACGTR 1 cut(s) 499
Psp6I CCWGG 2 cut(s) 401, 549
PspEI GGTNACC 1 cut(s) 241
PspGI CCWGG 2 cut(s) 401, 549
PspN4I GGNNCC 1 cut(s) 524
PspPI GGNCC 1 cut(s) 405
PstI CTGCAG 1 cut(s) 191
PvuII CAGCTG 1 cut(s) 62
RsaI GTAC 2 cut(s) 185, 438
RsaNI GTAC 2 cut(s) 184, 437
SatI GCNGC 2 cut(s) 60, 692
Sau3AI GATC 2 cut(s) 37, 267
Sau96I GGNCC 1 cut(s) 405
ScaI AGTACT 1 cut(s) 438
ScrFI CCNGG 3 cut(s) 130, 403, 551
SduI GDGCHC 3 cut(s) 75, 346, 538
SfcI CTRYAG 1 cut(s) 187
Sse9I AATT 1 cut(s) 627
SsiI CCGC 3 cut(s) 78, 271, 567
SspMI CTAG 3 cut(s) 288, 350, 422
StyD4I CCNGG 3 cut(s) 128, 401, 549
StyI CCWWGG 1 cut(s) 585
TaaI ACNGT 1 cut(s) 339
TaiI ACGT 1 cut(s) 501
TasI AATT 1 cut(s) 627
TatI WGTACW 2 cut(s) 183, 436
TfiI GAWTC 2 cut(s) 24, 84
TscAI CASTG 2 cut(s) 196, 243
TseFI GTSAC 1 cut(s) 241
TseI GCWGC 2 cut(s) 59, 691
Tsp45I GTSAC 1 cut(s) 241
TspDTI ATGAA 1 cut(s) 125
TspGWI ACGGA 2 cut(s) 79, 357
TspRI CASTG 2 cut(s) 196, 243
Van91I CCANNNNNTGG 1 cut(s) 252
VneI GTGCAC 1 cut(s) 71
XbaI TCTAGA 1 cut(s) 421
XspI CTAG 3 cut(s) 288, 350, 422
ZrmI AGTACT 1 cut(s) 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.