Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb2

Orthogroup ID

OG0005848

Physical Location & Seq

Genomic Coordinates Forward (+)

4868255 .. 4872218

Gene Structure 3964 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_2g05880.t3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1371 bp

ATGGAGTCTGTGTGTTCAGTTCTCTCCTTCTCTTACCCAAAGCTTACTTCAATGGCTACTCTTCATTCTTCTTTCCGTATCAAGAACGTGGCCTCCTCTGGCCAGAGAGTTGCAGCTACTGTAAAAACATCTGAAATTCCAAATAAAACCCTCAACCCGTTTGAAGTAATTTTGGGTACAAGCACTGCTGCTTCTTTACCTCCCAAGAAGGTTCTGGTGCCGATTGGGTTTGGGACTGAAGAAATGGAGGCTGTGATTATGGTGGATGTTCTGCGGCGAGCGGGTGCTGATGTCACCGTGGCCTCGGTGGAGCCACAGCTCCAAGTTGAAGCTTCTTGTGGGACTAAGTTGGTTGCTGATACCTCCATTTCCTCCTGTTCTGATCAAATTTTTGATCTTGTGGCTTTACCAGGAGGAATGCCTGGCTCGGTGAGATTAAGGGACTGCGAAGTACTTGAGAAAATTACAAGACAACAGGCTGAGAAAAGGAGGTTATATGGAGCTATATGTGCTGCTCCAGCAGTAACACTTCTCCCGTGGGGTCTTCTAAGGAGAAAGCAGACAACTTGCCACCCTGCATTCTTTGACAAGCTACCAACCTTCTGGGCAGTCAAATCAAACATTCAAGTCTCTGGAGTGCTCACAACAAGCCGTGGCCCCGGAACTTGTTTTGTGTTTGCGCTGTGCCTAGTGGAACAGCTATTTGGGGAATCTGTCGCCAAGGAAATTGGAGAGTTGCTGTTGGTGGGACCTGCTGATGATGAATCTAGAAAGGAGGAGTTCAACAAAATTGAATGGTCTTTTGATCACATTCCTCGAGTTCTCATTCCAGTTGCAAATGGTTCTGAAGTGATTGAAGTAGTGACTATTGTAGATATTCTGCGGCGAGCAAAGGTGGACATAACTGTTGCTTCAGTTGAAAAATCTGTGCAGATTTTAGCATCTCAAGGCACCAAAATCATAGCTGACAAGTTAATTGGTGTAGCTGCCAAGTCAACTTATGATTTGATCGTTCTGCCGGGAGGAAATGCTGGGGCTGAGCGGCTAAGCAGATCTAGAATTCTAAAGAGTCTCCTCAAAGAACAAGAACTAGCTGGAAGAATGTATGGAGCAGTATGTTCCTCATCAGCAATTCTACTTAAACAGGGCTTACTGGAGGGTAAGAAAGCCATAGCTCATCCTTCCATTGTGCGCAAACCTACAGATGAGTTGGTGAACAACACCAAAGTAGTAATTGATGGTAACCTGATCACAAGCAGTGGACTTGCCACTGTCGCAGAATTTGCATTGGCTATTGTGAGCAAACTGTATGGTCATGCAAGGGCAAGAAGTGTTGCAGAAGGCCTTGTTTATGAGTACCCGAGGACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0008150 GO:0009507 GO:0009536 GO:0009657 GO:0009658 GO:0009987 GO:0016043 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044444 GO:0044464 GO:0071840

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DJ-1_PfpI

Protein Analysis

457

Amino Acids

48.96

Weight (kDa)

8.74

Isoelectric Point (pI)

40.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DJ-1_PfpI	PF01965	3 - 94	8.8e-17	DJ-1/PfpI family
DJ-1_PfpI	PF01965	134 - 295	2.6e-35	DJ-1/PfpI family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0005848)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G34020 AT4G34020 AT4G34020 AT4G34020
fragaria_vesca	FvH4_2g05880 FvH4_2g05880 FvH4_2g05880 FvH4_2g05880 FvH4_2g05880 FvH4_2g05880
malus_domestica	MD05G1082200.v1.1
prunus_persica	Prupe.8G126700_v2.0.a1 Prupe.8G126700_v2.0.a1
pyrus_communis	pycom03g18750 pycom05g07700
rosa_chinensis	RchiOBHm_Chr6g0257661
rosa_laevigata	RLG00000014715
rosa_multiflora	Rmu_co8071652.1_g000001 Rmu_co8413389.1_g000001 Rmu_sc0026590.1_g000001
rosa_rugosa	Rorug05G0569400 Rorug05G0569500 Rorug05G0569600
rosa_samantha	Rh6AG086100 Rh6BG080200 Rh6CG075200 Rh6DG072100
rosa_wichuraiana	Rw6G007710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1193
Acc36I	ACCTGC	1 cut(s)	760
AccB1I	GGYRCC	2 cut(s)	217, 950
AccBSI	CCGCTC	2 cut(s)	281, 1042
AciI	CCGC	4 cut(s)	274, 281, 883, 1042
AcoI	YGGCCR	1 cut(s)	100
AcsI	RAATTY	4 cut(s)	135, 387, 1059, 1280
AcuI	CTGAAG	3 cut(s)	258, 867, 897
AfaI	GTAC	3 cut(s)	178, 453, 1358
AgsI	TTSAA	8 cut(s)	51, 164, 329, 626, 784, 794, 857, 920
AjnI	CCWGG	2 cut(s)	409, 421
AjuI	GAANNNNNNNTTGG	4 cut(s)	31, 63, 687, 719
AloI	GAACNNNNNNTCC	2 cut(s)	77, 109
Alw21I	GWGCWC	1 cut(s)	642
Alw26I	GTCTC	2 cut(s)	634, 1076
AlwNI	CAGNNNCTG	1 cut(s)	119
Ama87I	CYCGRG	2 cut(s)	816, 1360
AoxI	GGCC	5 cut(s)	90, 100, 300, 655, 1342
ApeKI	GCWGC	4 cut(s)	113, 188, 512, 986
ApoI	RAATTY	4 cut(s)	135, 387, 1059, 1280
AspLEI	GCGC	2 cut(s)	682, 1194
AspS9I	GGNCC	2 cut(s)	656, 749
AsuC2I	CCSGG	2 cut(s)	660, 1020
AsuHPI	GGTGA	3 cut(s)	286, 442, 1225
AvaI	CYCGRG	2 cut(s)	816, 1360
AvaII	GGWCC	1 cut(s)	749
BalI	TGGCCA	1 cut(s)	102
BanI	GGYRCC	2 cut(s)	217, 950
BbsI	GAAGAC	1 cut(s)	536
Bbv12I	GWGCWC	1 cut(s)	642
BbvI	GCAGC	4 cut(s)	125, 175, 499, 973
BccI	CCATC	1 cut(s)	1232
BceAI	ACGGC	1 cut(s)	636
BciT130I	CCWGG	2 cut(s)	411, 423
BclI	TGATCA	3 cut(s)	382, 805, 1248
BcnI	CCSGG	2 cut(s)	660, 1020
BcoDI	GTCTC	2 cut(s)	634, 1076
BfaI	CTAG	4 cut(s)	689, 768, 1056, 1091
BfmI	CTRYAG	1 cut(s)	1200
BfuAI	ACCTGC	1 cut(s)	760
BglII	AGATCT	1 cut(s)	1052
BisI	GCNGC	7 cut(s)	114, 189, 275, 513, 884, 987, 1043
BlpI	GCTNAGC	2 cut(s)	1038, 1046
BlsI	GCNGC	7 cut(s)	115, 190, 276, 514, 885, 988, 1044
BmcAI	AGTACT	1 cut(s)	453
Bme1390I	CCNGG	4 cut(s)	411, 423, 660, 1020
Bme18I	GGWCC	1 cut(s)	749
BmeT110I	CYCGRG	2 cut(s)	816, 1360
BmgT120I	GGNCC	2 cut(s)	656, 749
BmiI	GGNNCC	5 cut(s)	219, 312, 658, 750, 952
BmrFI	CCNGG	4 cut(s)	411, 423, 660, 1020
BmsI	GCATC	1 cut(s)	950
BpiI	GAAGAC	1 cut(s)	536
BpmI	CTGGAG	3 cut(s)	501, 654, 1175
Bpu1102I	GCTNAGC	2 cut(s)	1038, 1046
BpuEI	CTTGAG	2 cut(s)	476, 930
BpuMI	CCSGG	2 cut(s)	660, 1020
BsaJI	CCNNGG	7 cut(s)	297, 303, 536, 652, 658, 720, 1361
BsaXI	ACNNNNNCTCC	3 cut(s)	26, 77, 107
Bse1I	ACTGG	2 cut(s)	830, 1158
BseBI	CCWGG	2 cut(s)	411, 423
BseDI	CCNNGG	7 cut(s)	297, 303, 536, 652, 658, 720, 1361
BseGI	GGATG	2 cut(s)	271, 1177
BseMII	CTCAG	2 cut(s)	471, 1029
BseNI	ACTGG	2 cut(s)	830, 1158
BseRI	GAGGAG	3 cut(s)	85, 791, 1064
BseXI	GCAGC	4 cut(s)	125, 175, 499, 973
BseYI	CCCAGC	1 cut(s)	1031
BsgI	GTGCAG	1 cut(s)	950
BshFI	GGCC	5 cut(s)	92, 102, 302, 657, 1344
BshNI	GGYRCC	2 cut(s)	217, 950
BsiHKAI	GWGCWC	1 cut(s)	642
BsiHKCI	CYCGRG	2 cut(s)	816, 1360
BsiSI	CCGG	2 cut(s)	660, 1019
BslFI	GGGAC	4 cut(s)	247, 355, 455, 762
BsmAI	GTCTC	2 cut(s)	634, 1076
BsmFI	GGGAC	4 cut(s)	247, 355, 455, 762
BsmI	GAATGC	2 cut(s)	423, 578
BsnI	GGCC	5 cut(s)	92, 102, 302, 657, 1344
BsoBI	CYCGRG	2 cut(s)	816, 1360
Bsp1286I	GDGCHC	1 cut(s)	642
Bsp143I	GATC	6 cut(s)	382, 394, 805, 1008, 1052, 1248
Bsp1720I	GCTNAGC	2 cut(s)	1038, 1046
BspACI	CCGC	4 cut(s)	274, 281, 883, 1042
BspANI	GGCC	5 cut(s)	92, 102, 302, 657, 1344
BspCNI	CTCAG	2 cut(s)	472, 1030
BspLI	GGNNCC	5 cut(s)	219, 312, 658, 750, 952
BspMI	ACCTGC	1 cut(s)	760
BspT107I	GGYRCC	2 cut(s)	217, 950
BsrBI	CCGCTC	2 cut(s)	281, 1042
BsrI	ACTGG	2 cut(s)	830, 1158
BssECI	CCNNGG	7 cut(s)	297, 303, 536, 652, 658, 720, 1361
BssMI	GATC	6 cut(s)	382, 394, 805, 1008, 1052, 1248
BssT1I	CCWWGG	1 cut(s)	720
Bst2UI	CCWGG	2 cut(s)	411, 423
Bst4CI	ACNGT	5 cut(s)	121, 298, 907, 1273, 1308
Bst6I	CTCTTC	1 cut(s)	66
BstAPI	GCANNNNNTGC	1 cut(s)	1283
BstC8I	GCNNGC	2 cut(s)	279, 888
BstDEI	CTNAG	5 cut(s)	345, 480, 548, 1038, 1046
BstDSI	CCRYGG	3 cut(s)	297, 536, 652
BstEII	GGTNACC	1 cut(s)	1241
BstF5I	GGATG	2 cut(s)	271, 1177
BstHHI	GCGC	2 cut(s)	682, 1194
BstKTI	GATC	6 cut(s)	385, 397, 808, 1011, 1055, 1251
BstMAI	GTCTC	2 cut(s)	634, 1076
BstMBI	GATC	6 cut(s)	382, 394, 805, 1008, 1052, 1248
BstMWI	GCNNNNNNNGC	4 cut(s)	509, 518, 1274, 1283
BstNI	CCWGG	2 cut(s)	411, 423
BstPI	GGTNACC	1 cut(s)	1241
BstSCI	CCNGG	4 cut(s)	409, 421, 658, 1018
BstSFI	CTRYAG	1 cut(s)	1200
BstV1I	GCAGC	4 cut(s)	125, 175, 499, 973
BstV2I	GAAGAC	1 cut(s)	536
BstX2I	RGATCY	1 cut(s)	1052
BstXI	CCANNNNNNTGG	1 cut(s)	603
BstYI	RGATCY	1 cut(s)	1052
BsuRI	GGCC	5 cut(s)	92, 102, 302, 657, 1344
BtgI	CCRYGG	3 cut(s)	297, 536, 652
BtsCI	GGATG	2 cut(s)	271, 1177
BtsI	GCAGTG	2 cut(s)	183, 1264
BtsIMutI	CAGTG	3 cut(s)	183, 1264, 1269
BveI	ACCTGC	1 cut(s)	760
Cac8I	GCNNGC	2 cut(s)	279, 888
CaiI	CAGNNNCTG	1 cut(s)	119
CfoI	GCGC	2 cut(s)	682, 1194
Cfr13I	GGNCC	2 cut(s)	656, 749
Csp6I	GTAC	3 cut(s)	177, 452, 1357
CviAII	CATG	1 cut(s)	1316
CviQI	GTAC	3 cut(s)	177, 452, 1357
DdeI	CTNAG	5 cut(s)	345, 480, 548, 1038, 1046
DpnI	GATC	6 cut(s)	384, 396, 807, 1010, 1054, 1250
DpnII	GATC	6 cut(s)	382, 394, 805, 1008, 1052, 1248
EaeI	YGGCCR	1 cut(s)	100
Eam1104I	CTCTTC	1 cut(s)	66
EarI	CTCTTC	1 cut(s)	66
Eco130I	CCWWGG	1 cut(s)	720
Eco147I	AGGCCT	1 cut(s)	1344
Eco47I	GGWCC	1 cut(s)	749
Eco57I	CTGAAG	3 cut(s)	258, 867, 897
Eco88I	CYCGRG	2 cut(s)	816, 1360
Eco91I	GGTNACC	1 cut(s)	1241
EcoO109I	RGGNCCY	1 cut(s)	749
EcoO65I	GGTNACC	1 cut(s)	1241
EcoRI	GAATTC	1 cut(s)	1059
EcoRII	CCWGG	2 cut(s)	409, 421
EcoT14I	CCWWGG	1 cut(s)	720
ErhI	CCWWGG	1 cut(s)	720
FaeI	CATG	1 cut(s)	1319
FaqI	GGGAC	4 cut(s)	247, 355, 455, 762
FatI	CATG	1 cut(s)	1315
FauI	CCCGC	1 cut(s)	274
FbaI	TGATCA	3 cut(s)	382, 805, 1248
Fnu4HI	GCNGC	7 cut(s)	114, 189, 275, 513, 884, 987, 1043
FokI	GGATG	2 cut(s)	278, 1164
Fsp4HI	GCNGC	7 cut(s)	114, 189, 275, 513, 884, 987, 1043
FspBI	CTAG	4 cut(s)	689, 768, 1056, 1091
FspI	TGCGCA	1 cut(s)	1193
GlaI	GCGC	2 cut(s)	681, 1193
GluI	GCNGC	7 cut(s)	114, 189, 275, 513, 884, 987, 1043
GsaI	CCCAGC	1 cut(s)	1035
GsuI	CTGGAG	3 cut(s)	501, 654, 1175
HaeIII	GGCC	5 cut(s)	92, 102, 302, 657, 1344
HapII	CCGG	2 cut(s)	660, 1019
HhaI	GCGC	2 cut(s)	682, 1194
Hin1II	CATG	1 cut(s)	1319
Hin6I	GCGC	2 cut(s)	680, 1192
HinP1I	GCGC	2 cut(s)	680, 1192
HincII	GTYRAC	1 cut(s)	996
HindII	GTYRAC	1 cut(s)	996
HindIII	AAGCTT	2 cut(s)	41, 330
HinfI	GANTC	4 cut(s)	5, 710, 764, 1069
HpaII	CCGG	2 cut(s)	660, 1019
HphI	GGTGA	3 cut(s)	286, 442, 1225
Hpy166II	GTNNAC	4 cut(s)	898, 996, 1216, 1262
Hpy188I	TCNGA	3 cut(s)	133, 382, 847
Hpy188III	TCNNGA	4 cut(s)	82, 633, 768, 1056
Hpy8I	GTNNAC	4 cut(s)	898, 996, 1216, 1262
HpyAV	CCTTC	5 cut(s)	37, 202, 610, 1191, 1334
HpyCH4III	ACNGT	5 cut(s)	121, 298, 907, 1273, 1308
HpyCH4IV	ACGT	1 cut(s)	87
HpyCH4V	TGCA	7 cut(s)	113, 578, 836, 931, 1286, 1319, 1337
HpyF10VI	GCNNNNNNNGC	4 cut(s)	509, 518, 1274, 1283
HpyF3I	CTNAG	5 cut(s)	345, 480, 548, 1038, 1046
HpySE526I	ACGT	1 cut(s)	87
Hsp92II	CATG	1 cut(s)	1319
HspAI	GCGC	2 cut(s)	680, 1192
Ksp22I	TGATCA	3 cut(s)	382, 805, 1248
Kzo9I	GATC	6 cut(s)	382, 394, 805, 1008, 1052, 1248
LmnI	GCTCC	5 cut(s)	310, 324, 500, 520, 1109
Lsp1109I	GCAGC	4 cut(s)	125, 175, 499, 973
LweI	GCATC	1 cut(s)	950
MaeI	CTAG	4 cut(s)	689, 768, 1056, 1091
MaeII	ACGT	1 cut(s)	87
MaeIII	GTNAC	4 cut(s)	292, 523, 862, 1241
MalI	GATC	6 cut(s)	384, 396, 807, 1010, 1054, 1250
MbiI	CCGCTC	2 cut(s)	281, 1042
MboI	GATC	6 cut(s)	382, 394, 805, 1008, 1052, 1248
MboII	GAAGA	5 cut(s)	53, 60, 251, 536, 1110
MflI	RGATCY	1 cut(s)	1052
MhlI	GDGCHC	1 cut(s)	642
MlsI	TGGCCA	1 cut(s)	102
MluNI	TGGCCA	1 cut(s)	102
MlyI	GAGTC	2 cut(s)	14, 1078
Mox20I	TGGCCA	1 cut(s)	102
MscI	TGGCCA	1 cut(s)	102
MseI	TTAA	4 cut(s)	437, 974, 1140, 1369
Msp20I	TGGCCA	1 cut(s)	102
MspI	CCGG	2 cut(s)	660, 1019
MspR9I	CCNGG	4 cut(s)	411, 423, 660, 1020
Mva1269I	GAATGC	2 cut(s)	423, 578
MvaI	CCWGG	2 cut(s)	411, 423
MwoI	GCNNNNNNNGC	4 cut(s)	509, 518, 1274, 1283
NciI	CCSGG	2 cut(s)	660, 1020
NdeII	GATC	6 cut(s)	382, 394, 805, 1008, 1052, 1248
NlaIII	CATG	1 cut(s)	1319
NlaIV	GGNNCC	5 cut(s)	219, 312, 658, 750, 952
NmuCI	GTSAC	2 cut(s)	292, 862
NsbI	TGCGCA	1 cut(s)	1193
PaeR7I	CTCGAG	1 cut(s)	816
PceI	AGGCCT	1 cut(s)	1344
PctI	GAATGC	2 cut(s)	423, 578
PfeI	GAWTC	2 cut(s)	710, 764
PkrI	GCNGC	7 cut(s)	115, 190, 276, 514, 885, 988, 1044
PleI	GAGTC	2 cut(s)	13, 1077
PpsI	GAGTC	2 cut(s)	13, 1077
PpuMI	RGGWCCY	1 cut(s)	749
Psp5II	RGGWCCY	1 cut(s)	749
Psp6I	CCWGG	2 cut(s)	409, 421
PspEI	GGTNACC	1 cut(s)	1241
PspFI	CCCAGC	1 cut(s)	1031
PspGI	CCWGG	2 cut(s)	409, 421
PspN4I	GGNNCC	5 cut(s)	219, 312, 658, 750, 952
PspPI	GGNCC	2 cut(s)	656, 749
PspPPI	RGGWCCY	1 cut(s)	749
PspXI	VCTCGAGB	1 cut(s)	816
PstNI	CAGNNNCTG	1 cut(s)	119
PsuI	RGATCY	1 cut(s)	1052
RsaI	GTAC	3 cut(s)	178, 453, 1358
RsaNI	GTAC	3 cut(s)	177, 452, 1357
SaqAI	TTAA	4 cut(s)	437, 974, 1140, 1369
SatI	GCNGC	7 cut(s)	114, 189, 275, 513, 884, 987, 1043
Sau3AI	GATC	6 cut(s)	382, 394, 805, 1008, 1052, 1248
Sau96I	GGNCC	2 cut(s)	656, 749
ScaI	AGTACT	1 cut(s)	453
SchI	GAGTC	2 cut(s)	14, 1078
ScrFI	CCNGG	4 cut(s)	411, 423, 660, 1020
SduI	GDGCHC	1 cut(s)	642
SfaNI	GCATC	1 cut(s)	950
SfcI	CTRYAG	1 cut(s)	1200
Sfr274I	CTCGAG	1 cut(s)	816
SinI	GGWCC	1 cut(s)	749
SlaI	CTCGAG	1 cut(s)	816
SmlI	CTYRAG	3 cut(s)	455, 816, 945
SmoI	CTYRAG	3 cut(s)	455, 816, 945
SseBI	AGGCCT	1 cut(s)	1344
SsiI	CCGC	4 cut(s)	274, 281, 883, 1042
SspMI	CTAG	4 cut(s)	689, 768, 1056, 1091
StuI	AGGCCT	1 cut(s)	1344
StyD4I	CCNGG	4 cut(s)	409, 421, 658, 1018
StyI	CCWWGG	1 cut(s)	720
TaaI	ACNGT	5 cut(s)	121, 298, 907, 1273, 1308
TaiI	ACGT	1 cut(s)	90
TaqI	TCGA	1 cut(s)	817
TatI	WGTACW	1 cut(s)	451
TauI	GCSGC	3 cut(s)	277, 886, 1045
TfiI	GAWTC	2 cut(s)	710, 764
Tru1I	TTAA	4 cut(s)	437, 974, 1140, 1369
Tru9I	TTAA	4 cut(s)	437, 974, 1140, 1369
TscAI	CASTG	3 cut(s)	190, 1264, 1276
TseFI	GTSAC	2 cut(s)	292, 862
TseI	GCWGC	4 cut(s)	113, 188, 512, 986
Tsp45I	GTSAC	2 cut(s)	292, 862
TspDTI	ATGAA	2 cut(s)	53, 777
TspGWI	ACGGA	1 cut(s)	65
TspRI	CASTG	3 cut(s)	190, 1264, 1276
VpaK11BI	GGWCC	1 cut(s)	749
XapI	RAATTY	4 cut(s)	135, 387, 1059, 1280
XbaI	TCTAGA	2 cut(s)	767, 1055
XcmI	CCANNNNNNNNNTGG	1 cut(s)	211
XhoI	CTCGAG	1 cut(s)	816
XspI	CTAG	4 cut(s)	689, 768, 1056, 1091
ZrmI	AGTACT	1 cut(s)	453

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.