Rorug05G0569600

DJ-1/PfpI family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0005848
Physical Location & Seq
Reverse (-)
76272051 .. 76273653
1603 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0569600.1

Sequence Viewer

Length: 1503 bp
ATGGCTTTTGAACAACAATATTCTATATGCATTCTGCTTGTCCTTCTACAGCTTCCCTTTTTCACCATTGCTCAAAGTTACAAAAACATATCTTTTAGCTCATCCCTTATTGCACAGGACAGTGACCCTCACTGGGCCTCGCCGTCTGGGGAATTTGCGTTCGGCTTCAGAAAAATTGGCAATGCTGGCTTCTTACTAGCCATTTGGTTTGCCAATATACATGAAAAGACTATAGTATGGTCAGCCAATCGCAATAATCCAGTGCAACAAGGATCAAAAGTTGAATTCTCTTTTGACGGCAAGTTTACTCTAACTGATATTAGAACAGACAAACGAACAAACATTGCTGATGGCCAGTCTACGGATACTACTGGAGTTGCCTATGCAGCCATGCTGGACACGGGAAATTTCGTTCTGGCAGCCCAAAATTCAACCTATTTGTGGCAGAGTTTTGATCATCCAACTGATACCATCCTTCCCACACAGACCCTTAATCTAAACAGCAGTCTCTTTGCCCAACTTACAGCAACAAATTACTCAGAAGGAAGATTCAATGGCTTTCAGGTCATCTTCAACCAATCTGGCTCTATATACCTCACAGCACAGAACGGAAGCACACTTCATGTGGTCTTAGAAGATCCAGTTTCCACTCAAGACGTCTACCAGAGAGCAACTCTTGACTATGATGGAGTTTTGAGGCACTATATGCACCAGAAAAGCACTGACTCTTCGAGTGCCTGGTCCACTTTTACCGTCATACCTTCAAATATTTGCACAGCAATTCAGGAATATACAGGTGGGGGTGCATGTGGGTTTAACAGCTTATGTAAACATGATAAGGATGCAGCTCATACTAATTGCTCCTGCCCTCCGAGTTACATCCTCATTGACCAAGATGATGAGAGGAAAGGGTGCACGCAAAACTTTGTTCCCCAAAGTTGTGATAAAGCCTCATCAGAAATAGACCTCTTTGAAGTTCAAGAGCTTCAATTCACTGATTGGCCAGGTGGAGATTATGAGCATTTCCAGCCAGTTAATAAGGAACAGTGCAAGCAAAATTGCTTAGCCGATTGTTTCTGTGCCATTGCCATTTTCAATGAAGGAAGTGGTGATTGTTGGAAGAAGCGAATCCCTCTTTCGAACGGGAGGATCAACTACGATGTTAAATGGTTATCTCTGCTGAAAATAAGGAAAGGCACAAAAGAGAAAGATAGTCTAACTCTGCTCATCATTGGAGCAGTGATCATTTTAATATCAACCAACTTGGTTGTTTTTATAATAACCCATCTGGTTACCTCTCATGCAAAGGTGAATCGACTTAATCCTGTGGTTCAAGGCATGAATCTGAAATGTTTCACTTTCATGGAGCTAAAAGAAGCCACCAATGGATTCAAGGAAGAGCTAGGTCGTGGTGCTTTTGCAACCGTTTTCAAAGGAGTTTTAGCATCTCATACTGGGAAGTTCATTGCTGTCAAGAGATTGAACACTGGTCAAAGAAAATGA

Protein Analysis

500

Amino Acids

55.59

Weight (kDa)

6.31

Isoelectric Point (pI)

38.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 77 - 169 1.2e-19 D-mannose binding lectin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1277
AatII GACGTC 1 cut(s) 660
AccI GTMKAC 2 cut(s) 359, 660
AclWI GGATC 3 cut(s) 280, 632, 1157
AcoI YGGCCR 2 cut(s) 352, 1001
AcsI RAATTY 4 cut(s) 152, 284, 406, 427
AcuI CTGAAG 1 cut(s) 151
AcyI GRCGYC 1 cut(s) 657
AfiI CCNNNNNNNGG 3 cut(s) 133, 361, 441
AjnI CCWGG 2 cut(s) 737, 1003
AloI GAACNNNNNNTCC 4 cut(s) 143, 175, 396, 428
AluBI AGCT 7 cut(s) 52, 99, 822, 848, 985, 1369, 1402
AluI AGCT 7 cut(s) 52, 99, 822, 848, 985, 1369, 1402
Alw21I GWGCWC 1 cut(s) 917
Alw26I GTCTC 1 cut(s) 512
Alw44I GTGCAC 1 cut(s) 913
AlwI GGATC 3 cut(s) 280, 632, 1157
AoxI GGCC 3 cut(s) 135, 352, 1001
ApaLI GTGCAC 1 cut(s) 913
ApeKI GCWGC 3 cut(s) 386, 419, 845
ApoI RAATTY 4 cut(s) 152, 284, 406, 427
Asp700I GAANNNNTTC 1 cut(s) 1352
AspS9I GGNCC 2 cut(s) 135, 741
AsuHPI GGTGA 3 cut(s) 55, 1121, 1321
AsuII TTCGAA 1 cut(s) 1139
AvaII GGWCC 1 cut(s) 741
BaeGI GKGCMC 1 cut(s) 917
BalI TGGCCA 2 cut(s) 354, 1003
Bbv12I GWGCWC 1 cut(s) 917
BbvI GCAGC 3 cut(s) 398, 431, 857
BccI CCATC 4 cut(s) 344, 479, 680, 1293
BceAI ACGGC 2 cut(s) 127, 313
BciT130I CCWGG 2 cut(s) 739, 1005
BciVI GTATCC 1 cut(s) 358
BclI TGATCA 2 cut(s) 454, 1242
BcoDI GTCTC 1 cut(s) 512
BfaI CTAG 2 cut(s) 197, 1403
BfmI CTRYAG 2 cut(s) 47, 231
BfuI GTATCC 1 cut(s) 358
BisI GCNGC 3 cut(s) 387, 420, 846
BlpI GCTNAGC 1 cut(s) 1063
BlsI GCNGC 3 cut(s) 388, 421, 847
Bme1390I CCNGG 2 cut(s) 739, 1005
Bme18I GGWCC 1 cut(s) 741
BmgT120I GGNCC 2 cut(s) 135, 741
BmrFI CCNGG 2 cut(s) 739, 1005
BmrI ACTGGG 2 cut(s) 142, 1464
BmsI GCATC 2 cut(s) 832, 1454
BmuI ACTGGG 2 cut(s) 142, 1464
BplI GAGNNNNNCTC 2 cut(s) 658, 690
BpmI CTGGAG 1 cut(s) 393
Bpu1102I GCTNAGC 1 cut(s) 1063
Bpu14I TTCGAA 1 cut(s) 1139
BpuEI CTTGAG 1 cut(s) 636
BsaHI GRCGYC 1 cut(s) 657
Bsc4I CCNNNNNNNGG 3 cut(s) 133, 361, 441
Bse1I ACTGG 8 cut(s) 137, 260, 355, 376, 641, 1031, 1459, 1492
Bse3DI GCAATG 5 cut(s) 66, 187, 342, 1083, 1464
BseBI CCWGG 2 cut(s) 739, 1005
BseGI GGATG 5 cut(s) 101, 457, 471, 847, 879
BseLI CCNNNNNNNGG 3 cut(s) 133, 361, 441
BseMI GCAATG 5 cut(s) 66, 187, 342, 1083, 1464
BseMII CTCAG 1 cut(s) 552
BseNI ACTGG 8 cut(s) 137, 260, 355, 376, 641, 1031, 1459, 1492
BseSI GKGCMC 1 cut(s) 917
BseXI GCAGC 3 cut(s) 398, 431, 857
BshFI GGCC 3 cut(s) 137, 354, 1003
BsiHKAI GWGCWC 1 cut(s) 917
BslI CCNNNNNNNGG 3 cut(s) 133, 361, 441
BsmAI GTCTC 1 cut(s) 512
BsmI GAATGC 1 cut(s) 30
BsnI GGCC 3 cut(s) 137, 354, 1003
Bsp119I TTCGAA 1 cut(s) 1139
Bsp1286I GDGCHC 1 cut(s) 917
Bsp143I GATC 5 cut(s) 272, 454, 637, 1149, 1242
Bsp1720I GCTNAGC 1 cut(s) 1063
BspANI GGCC 3 cut(s) 137, 354, 1003
BspCNI CTCAG 1 cut(s) 551
BspPI GGATC 3 cut(s) 280, 632, 1157
BspQI GCTCTTC 1 cut(s) 1392
BspT104I TTCGAA 1 cut(s) 1139
BsrDI GCAATG 5 cut(s) 66, 187, 342, 1083, 1464
BsrI ACTGG 8 cut(s) 137, 260, 355, 376, 641, 1031, 1459, 1492
BssMI GATC 5 cut(s) 272, 454, 637, 1149, 1242
BssNI GRCGYC 1 cut(s) 657
Bst2UI CCWGG 2 cut(s) 739, 1005
Bst4CI ACNGT 4 cut(s) 122, 754, 1047, 1426
Bst6I CTCTTC 2 cut(s) 733, 1392
BstACI GRCGYC 1 cut(s) 657
BstAPI GCANNNNNTGC 1 cut(s) 706
BstBI TTCGAA 1 cut(s) 1139
BstC8I GCNNGC 3 cut(s) 187, 917, 1052
BstDEI CTNAG 3 cut(s) 538, 631, 1063
BstEII GGTNACC 1 cut(s) 1291
BstF5I GGATG 5 cut(s) 101, 457, 471, 847, 879
BstKTI GATC 5 cut(s) 275, 457, 640, 1152, 1245
BstMAI GTCTC 1 cut(s) 512
BstMBI GATC 5 cut(s) 272, 454, 637, 1149, 1242
BstMWI GCNNNNNNNGC 4 cut(s) 186, 386, 706, 1027
BstNI CCWGG 2 cut(s) 739, 1005
BstNSI RCATGY 1 cut(s) 810
BstPI GGTNACC 1 cut(s) 1291
BstSCI CCNGG 2 cut(s) 737, 1003
BstSFI CTRYAG 2 cut(s) 47, 231
BstSLI GKGCMC 1 cut(s) 917
BstV1I GCAGC 3 cut(s) 398, 431, 857
BstX2I RGATCY 1 cut(s) 637
BstYI RGATCY 1 cut(s) 637
BsuI GTATCC 1 cut(s) 358
BsuRI GGCC 3 cut(s) 137, 354, 1003
BtsCI GGATG 5 cut(s) 101, 457, 471, 847, 879
BtsI GCAGTG 1 cut(s) 1245
BtsIMutI CAGTG 8 cut(s) 127, 130, 267, 720, 993, 1052, 1245, 1485
Cac8I GCNNGC 3 cut(s) 187, 917, 1052
Cfr13I GGNCC 2 cut(s) 135, 741
CviAII CATG 8 cut(s) 221, 391, 623, 807, 833, 1301, 1339, 1363
DdeI CTNAG 3 cut(s) 538, 631, 1063
DpnI GATC 5 cut(s) 274, 456, 639, 1151, 1244
DpnII GATC 5 cut(s) 272, 454, 637, 1149, 1242
EaeI YGGCCR 2 cut(s) 352, 1001
Eam1104I CTCTTC 2 cut(s) 733, 1392
EarI CTCTTC 2 cut(s) 733, 1392
Eco47I GGWCC 1 cut(s) 741
Eco57I CTGAAG 1 cut(s) 151
Eco91I GGTNACC 1 cut(s) 1291
EcoO65I GGTNACC 1 cut(s) 1291
EcoRI GAATTC 1 cut(s) 284
EcoRII CCWGG 2 cut(s) 737, 1003
EcoT22I ATGCAT 1 cut(s) 32
FaeI CATG 8 cut(s) 224, 394, 626, 810, 836, 1304, 1342, 1366
FatI CATG 8 cut(s) 220, 390, 622, 806, 832, 1300, 1338, 1362
FbaI TGATCA 2 cut(s) 454, 1242
FblI GTMKAC 2 cut(s) 359, 660
Fnu4HI GCNGC 3 cut(s) 387, 420, 846
FokI GGATG 5 cut(s) 88, 444, 458, 854, 866
Fsp4HI GCNGC 3 cut(s) 387, 420, 846
FspBI CTAG 2 cut(s) 197, 1403
GluI GCNGC 3 cut(s) 387, 420, 846
GsuI CTGGAG 1 cut(s) 393
HaeIII GGCC 3 cut(s) 137, 354, 1003
Hin1I GRCGYC 1 cut(s) 657
Hin1II CATG 8 cut(s) 224, 394, 626, 810, 836, 1304, 1342, 1366
HinfI GANTC 6 cut(s) 549, 725, 1128, 1312, 1342, 1389
HphI GGTGA 3 cut(s) 55, 1121, 1321
Hpy166II GTNNAC 6 cut(s) 306, 360, 661, 744, 830, 915
Hpy188I TCNGA 5 cut(s) 170, 541, 873, 958, 1347
Hpy188III TCNNGA 5 cut(s) 653, 677, 785, 980, 1474
Hpy8I GTNNAC 6 cut(s) 306, 360, 661, 744, 830, 915
HpyAV CCTTC 5 cut(s) 53, 485, 536, 771, 1094
HpyCH4III ACNGT 4 cut(s) 122, 754, 1047, 1426
HpyCH4IV ACGT 1 cut(s) 657
HpyF10VI GCNNNNNNNGC 4 cut(s) 186, 386, 706, 1027
HpyF3I CTNAG 3 cut(s) 538, 631, 1063
HpySE526I ACGT 1 cut(s) 657
Hsp92I GRCGYC 1 cut(s) 657
Hsp92II CATG 8 cut(s) 224, 394, 626, 810, 836, 1304, 1342, 1366
Ksp22I TGATCA 2 cut(s) 454, 1242
Kzo9I GATC 5 cut(s) 272, 454, 637, 1149, 1242
LguI GCTCTTC 1 cut(s) 1392
LmnI GCTCC 3 cut(s) 866, 1235, 1366
Lsp1109I GCAGC 3 cut(s) 398, 431, 857
LweI GCATC 2 cut(s) 832, 1454
MaeI CTAG 2 cut(s) 197, 1403
MaeII ACGT 1 cut(s) 657
MaeIII GTNAC 4 cut(s) 77, 122, 875, 1291
MalI GATC 5 cut(s) 274, 456, 639, 1151, 1244
MboI GATC 5 cut(s) 272, 454, 637, 1149, 1242
MboII GAAGA 6 cut(s) 558, 562, 647, 720, 1132, 1409
MflI RGATCY 1 cut(s) 637
MhlI GDGCHC 1 cut(s) 917
MlsI TGGCCA 2 cut(s) 354, 1003
MluNI TGGCCA 2 cut(s) 354, 1003
MlyI GAGTC 1 cut(s) 719
MmeI TCCRAC 2 cut(s) 485, 1097
Mox20I TGGCCA 2 cut(s) 354, 1003
Mph1103I ATGCAT 1 cut(s) 32
MroXI GAANNNNTTC 1 cut(s) 1352
MscI TGGCCA 2 cut(s) 354, 1003
MseI TTAA 6 cut(s) 492, 816, 1035, 1164, 1250, 1320
MslI CAYNNNNRTG 1 cut(s) 1361
Msp20I TGGCCA 2 cut(s) 354, 1003
MspR9I CCNGG 2 cut(s) 739, 1005
Mva1269I GAATGC 1 cut(s) 30
MvaI CCWGG 2 cut(s) 739, 1005
MwoI GCNNNNNNNGC 4 cut(s) 186, 386, 706, 1027
NdeII GATC 5 cut(s) 272, 454, 637, 1149, 1242
NlaIII CATG 8 cut(s) 224, 394, 626, 810, 836, 1304, 1342, 1366
NmuCI GTSAC 1 cut(s) 122
NsiI ATGCAT 1 cut(s) 32
NspI RCATGY 1 cut(s) 810
NspV TTCGAA 1 cut(s) 1139
PciSI GCTCTTC 1 cut(s) 1392
PctI GAATGC 1 cut(s) 30
PdmI GAANNNNTTC 1 cut(s) 1352
PfeI GAWTC 5 cut(s) 549, 1128, 1312, 1342, 1389
PkrI GCNGC 3 cut(s) 388, 421, 847
PleI GAGTC 1 cut(s) 719
PpsI GAGTC 1 cut(s) 719
PsiI TTATAA 1 cut(s) 1277
Psp6I CCWGG 2 cut(s) 737, 1003
PspEI GGTNACC 1 cut(s) 1291
PspGI CCWGG 2 cut(s) 737, 1003
PspPI GGNCC 2 cut(s) 135, 741
PsuI RGATCY 1 cut(s) 637
RseI CAYNNNNRTG 1 cut(s) 1361
SapI GCTCTTC 1 cut(s) 1392
SaqAI TTAA 6 cut(s) 492, 816, 1035, 1164, 1250, 1320
SatI GCNGC 3 cut(s) 387, 420, 846
Sau3AI GATC 5 cut(s) 272, 454, 637, 1149, 1242
Sau96I GGNCC 2 cut(s) 135, 741
SchI GAGTC 1 cut(s) 719
ScrFI CCNGG 2 cut(s) 739, 1005
SduI GDGCHC 1 cut(s) 917
SfaNI GCATC 2 cut(s) 832, 1454
SfcI CTRYAG 2 cut(s) 47, 231
SfuI TTCGAA 1 cut(s) 1139
SinI GGWCC 1 cut(s) 741
SmiMI CAYNNNNRTG 1 cut(s) 1361
SmlI CTYRAG 1 cut(s) 651
SmoI CTYRAG 1 cut(s) 651
SspI AATATT 2 cut(s) 20, 769
SspMI CTAG 2 cut(s) 197, 1403
StyD4I CCNGG 2 cut(s) 737, 1003
TaaI ACNGT 4 cut(s) 122, 754, 1047, 1426
TaiI ACGT 1 cut(s) 660
TaqI TCGA 3 cut(s) 731, 1139, 1315
TfiI GAWTC 5 cut(s) 549, 1128, 1312, 1342, 1389
Tru1I TTAA 6 cut(s) 492, 816, 1035, 1164, 1250, 1320
Tru9I TTAA 6 cut(s) 492, 816, 1035, 1164, 1250, 1320
TscAI CASTG 8 cut(s) 127, 137, 267, 727, 1000, 1052, 1245, 1492
TseFI GTSAC 1 cut(s) 122
TseI GCWGC 3 cut(s) 386, 419, 845
Tsp45I GTSAC 1 cut(s) 122
TspDTI ATGAA 6 cut(s) 237, 611, 1113, 1351, 1355, 1453
TspGWI ACGGA 2 cut(s) 377, 624
TspRI CASTG 8 cut(s) 127, 137, 267, 727, 1000, 1052, 1245, 1492
VneI GTGCAC 1 cut(s) 913
VpaK11BI GGWCC 1 cut(s) 741
XapI RAATTY 4 cut(s) 152, 284, 406, 427
XceI RCATGY 1 cut(s) 810
XmiI GTMKAC 2 cut(s) 359, 660
XmnI GAANNNNTTC 1 cut(s) 1352
XspI CTAG 2 cut(s) 197, 1403
ZraI GACGTC 1 cut(s) 658
Zsp2I ATGCAT 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.