Rorug05G0569400

DJ-1/PfpI family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0005848
Physical Location & Seq
Reverse (-)
76264694 .. 76265992
1299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0569400.1

Sequence Viewer

Length: 1299 bp
ATGGAAGGGGCTTTTACTGTATGGCTGCGTCTGTTGCTTATTTCCTCATTGTTTCTGGCCTCAATTTCTGTGGATAAAATAGCAGTGGGTGATTATCTCTCTGCAACTGCAGGTCACAACTCCTCATGGCTTTCTCCTTCTGGTGATTTTGCCTTTGGATTTCGCCAACTTGAAAACAATGAGTATTTCTTGCTTTCTATATGGTTTGCCAAGATACTAGACAGAACCATAGTTTGGTATGCAAATGGAGATAGGCCTGCACCTAAGGGCTCAATTGTGAACTTGACTTCCAGCAGTGGATTAGTGCTTACAACTCCTCAGGGTAAACAGCTATGGAAATCTGATCTAACTATTGCTGGCTTTGTTGCTGATGGGGTTATGAATGATAAAGGTAACTTTGTTCTGGAAGATGAGAAGTCAGAAAAGTTATGGGAGACATTCAAGAATCCTACTGATACCATGTTGCCTGGGCAAATATTAGAAAAAGGAGGGAGACTTTCTTCTCGACAATCAGAGACTAACTATGCGAGAGGACGCTTTCAGATAGATTTTCAAGAAGATGGGAATCTTGTGTTTAGTGCCAAAAAACCTTTTGATTCTACTGCAACTACCAAGGGGACTGTTCCAGGTAGTGAAGGTAAACAACTGGTTTTCAATGAGTCAGGCAACTTCTATATTCTGCGAGTGAATGGTAGAAGATTCACTTTCACAGCAGAGGAACGCCTAGCAAGGGACTACTATCTCCGGTCAACTCTCAACTTTGATGGGGTTTTGGTTCAATATTTTCTCCCAAAGTCTTTTGTTGACACTGTAAGCTGGAAACCACTTTGGTCAGAACCAGATAACATTTGCCAGAAAGTTGATTCAGGTGCGGGTGTATGTGGGTACAACAGTATCTGCACTCTGAAAACAGATCAAAGGCCAACCTGTAAATGCCCAAGAGGATACTCTTTGCTTGATCCAAATGATCCGTACGGGAGCTGCAATCCAAATTTCAGACATGGCTGTGAAGATGAGCCTAGTTTTTCAAAAGATCTCTTTGATGTTCAGGTGCTACCAAATACTGACTGGCTTACCCCCGGTTATGAGCTGTTAAATGCTTCTACTGCAGACAAATGCAATCAATATTGCAAGCAAGATTGTTTGTGTGATGTTGCTATCTACCGCAATGAAACCTGTTGGAAAAAGAAATTACCTCTCTCATATGGGAGAGAAGACAGTAGTCTGAATGCAAAGACCTTCATCAAATTCAGGAAGGATAATTCAACTCTACCGGCTCCACCATGTCGACCTCCATAA

Protein Analysis

432

Amino Acids

48.47

Weight (kDa)

6.26

Isoelectric Point (pI)

36.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
B_lectin PF01453 76 - 161 1.5e-13 D-mannose binding lectin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 101
AccB7I CCANNNNNTGG 1 cut(s) 234
AccI GTMKAC 1 cut(s) 1288
AciI CCGC 2 cut(s) 872, 1165
AclWI GGATC 2 cut(s) 953, 962
AcsI RAATTY 2 cut(s) 991, 1247
AfaI GTAC 2 cut(s) 887, 974
AfiI CCNNNNNNNGG 2 cut(s) 234, 730
AgsI TTSAA 7 cut(s) 173, 442, 554, 655, 779, 1029, 1266
AjnI CCWGG 2 cut(s) 466, 625
AjuI GAANNNNNNNTTGG 2 cut(s) 217, 249
AloI GAACNNNNNNTCC 2 cut(s) 272, 304
AluBI AGCT 4 cut(s) 331, 816, 981, 1090
AluI AGCT 4 cut(s) 331, 816, 981, 1090
Alw26I GTCTC 3 cut(s) 428, 487, 509
AlwI GGATC 2 cut(s) 953, 962
AlwNI CAGNNNCTG 1 cut(s) 897
AoxI GGCC 3 cut(s) 57, 254, 920
ApeKI GCWGC 2 cut(s) 25, 981
ApoI RAATTY 2 cut(s) 991, 1247
AsuC2I CCSGG 1 cut(s) 1080
AsuHPI GGTGA 2 cut(s) 101, 155
AxyI CCTNAGG 2 cut(s) 264, 318
BaeI ACNNNNGTAYC 4 cut(s) 877, 877, 910, 910
BanII GRGCYC 1 cut(s) 272
BbsI GAAGAC 1 cut(s) 1221
BbvI GCAGC 2 cut(s) 12, 968
BccI CCATC 3 cut(s) 365, 554, 758
BciT130I CCWGG 2 cut(s) 468, 627
BciVI GTATCC 1 cut(s) 938
BcnI CCSGG 1 cut(s) 1080
BcoDI GTCTC 3 cut(s) 428, 487, 509
BfaI CTAG 3 cut(s) 218, 725, 1020
BfmI CTRYAG 2 cut(s) 108, 1107
BfuAI ACCTGC 1 cut(s) 101
BfuI GTATCC 1 cut(s) 938
BglII AGATCT 1 cut(s) 1033
BisI GCNGC 2 cut(s) 26, 982
BlsI GCNGC 2 cut(s) 27, 983
Bme1390I CCNGG 3 cut(s) 468, 627, 1080
BmiI GGNNCC 1 cut(s) 1278
BmrFI CCNGG 3 cut(s) 468, 627, 1080
BoxI GACNNNNGTC 1 cut(s) 1221
BpiI GAAGAC 1 cut(s) 1221
BpuMI CCSGG 1 cut(s) 1080
BsaBI GATNNNNATC 1 cut(s) 564
BsaJI CCNNGG 3 cut(s) 467, 612, 1078
BsaWI WCCGGW 1 cut(s) 744
Bsc4I CCNNNNNNNGG 2 cut(s) 234, 730
Bse118I RCCGGY 1 cut(s) 1273
Bse1I ACTGG 2 cut(s) 651, 1073
Bse21I CCTNAGG 2 cut(s) 264, 318
Bse3DI GCAATG 1 cut(s) 1174
Bse8I GATNNNNATC 1 cut(s) 564
BseBI CCWGG 2 cut(s) 468, 627
BseDI CCNNGG 3 cut(s) 467, 612, 1078
BseJI GATNNNNATC 1 cut(s) 564
BseLI CCNNNNNNNGG 2 cut(s) 234, 730
BseMI GCAATG 1 cut(s) 1174
BseMII CTCAG 1 cut(s) 332
BseNI ACTGG 2 cut(s) 651, 1073
BseRI GAGGAG 2 cut(s) 112, 306
BseXI GCAGC 2 cut(s) 12, 968
BsgI GTGCAG 2 cut(s) 243, 883
BshFI GGCC 3 cut(s) 59, 256, 922
BsiSI CCGG 3 cut(s) 745, 1080, 1274
BsiWI CGTACG 1 cut(s) 972
BslFI GGGAC 2 cut(s) 631, 746
BslI CCNNNNNNNGG 2 cut(s) 234, 730
BsmAI GTCTC 3 cut(s) 428, 487, 509
BsmFI GGGAC 2 cut(s) 631, 746
BsmI GAATGC 1 cut(s) 1234
BsnI GGCC 3 cut(s) 59, 256, 922
Bsp1286I GDGCHC 1 cut(s) 272
Bsp143I GATC 5 cut(s) 343, 913, 958, 967, 1033
BspACI CCGC 2 cut(s) 872, 1165
BspANI GGCC 3 cut(s) 59, 256, 922
BspCNI CTCAG 1 cut(s) 331
BspLI GGNNCC 1 cut(s) 1278
BspMAI CTGCAG 2 cut(s) 112, 1111
BspMI ACCTGC 1 cut(s) 101
BspPI GGATC 2 cut(s) 953, 962
BsrDI GCAATG 1 cut(s) 1174
BsrFI RCCGGY 1 cut(s) 1273
BsrI ACTGG 2 cut(s) 651, 1073
BssAI RCCGGY 1 cut(s) 1273
BssECI CCNNGG 3 cut(s) 467, 612, 1078
BssMI GATC 5 cut(s) 343, 913, 958, 967, 1033
BssT1I CCWWGG 1 cut(s) 612
Bst2UI CCWGG 2 cut(s) 468, 627
Bst4CI ACNGT 5 cut(s) 19, 622, 811, 893, 1220
BstC8I GCNNGC 3 cut(s) 258, 358, 1133
BstDEI CTNAG 2 cut(s) 264, 318
BstKTI GATC 5 cut(s) 346, 916, 961, 970, 1036
BstMAI GTCTC 3 cut(s) 428, 487, 509
BstMBI GATC 5 cut(s) 343, 913, 958, 967, 1033
BstMWI GCNNNNNNNGC 2 cut(s) 34, 1106
BstNI CCWGG 2 cut(s) 468, 627
BstPAI GACNNNNGTC 1 cut(s) 1221
BstSCI CCNGG 3 cut(s) 466, 625, 1078
BstSFI CTRYAG 2 cut(s) 108, 1107
BstV1I GCAGC 2 cut(s) 12, 968
BstV2I GAAGAC 1 cut(s) 1221
BstX2I RGATCY 1 cut(s) 1033
BstYI RGATCY 1 cut(s) 1033
Bsu36I CCTNAGG 2 cut(s) 264, 318
BsuI GTATCC 1 cut(s) 938
BsuRI GGCC 3 cut(s) 59, 256, 922
BtsI GCAGTG 2 cut(s) 90, 301
BtsIMutI CAGTG 3 cut(s) 90, 301, 807
BveI ACCTGC 1 cut(s) 101
Cac8I GCNNGC 3 cut(s) 258, 358, 1133
CaiI CAGNNNCTG 1 cut(s) 897
Cfr10I RCCGGY 1 cut(s) 1273
CseI GACGC 2 cut(s) 17, 543
Csp6I GTAC 2 cut(s) 886, 973
CspCI CAANNNNNGTGG 2 cut(s) 51, 86
CviAII CATG 4 cut(s) 126, 460, 1001, 1284
CviQI GTAC 2 cut(s) 886, 973
DdeI CTNAG 2 cut(s) 264, 318
DpnI GATC 5 cut(s) 345, 915, 960, 969, 1035
DpnII GATC 5 cut(s) 343, 913, 958, 967, 1033
Eco130I CCWWGG 1 cut(s) 612
Eco147I AGGCCT 1 cut(s) 256
Eco24I GRGCYC 1 cut(s) 272
Eco81I CCTNAGG 2 cut(s) 264, 318
EcoRII CCWGG 2 cut(s) 466, 625
EcoT14I CCWWGG 1 cut(s) 612
EcoT38I GRGCYC 1 cut(s) 272
ErhI CCWWGG 1 cut(s) 612
FaeI CATG 4 cut(s) 129, 463, 1004, 1287
FalI AAGNNNNNCTT 2 cut(s) 688, 720
FaqI GGGAC 2 cut(s) 631, 746
FatI CATG 4 cut(s) 125, 459, 1000, 1283
FauI CCCGC 1 cut(s) 865
FauNDI CATATG 1 cut(s) 1204
FblI GTMKAC 1 cut(s) 1288
Fnu4HI GCNGC 2 cut(s) 26, 982
FriOI GRGCYC 1 cut(s) 272
Fsp4HI GCNGC 2 cut(s) 26, 982
FspBI CTAG 3 cut(s) 218, 725, 1020
GluI GCNGC 2 cut(s) 26, 982
HaeIII GGCC 3 cut(s) 59, 256, 922
HapII CCGG 3 cut(s) 745, 1080, 1274
HgaI GACGC 2 cut(s) 17, 543
Hin1II CATG 4 cut(s) 129, 463, 1004, 1287
HincII GTYRAC 3 cut(s) 750, 805, 1289
HindII GTYRAC 3 cut(s) 750, 805, 1289
HinfI GANTC 6 cut(s) 445, 565, 596, 659, 699, 863
HpaII CCGG 3 cut(s) 745, 1080, 1274
HphI GGTGA 2 cut(s) 101, 155
Hpy166II GTNNAC 6 cut(s) 280, 326, 641, 750, 805, 1289
Hpy188I TCNGA 8 cut(s) 343, 421, 514, 543, 835, 906, 998, 1227
Hpy188III TCNNGA 5 cut(s) 404, 442, 504, 554, 1252
Hpy8I GTNNAC 6 cut(s) 280, 326, 641, 750, 805, 1289
HpyAV CCTTC 4 cut(s) 147, 629, 1249, 1249
HpyCH4III ACNGT 5 cut(s) 19, 622, 811, 893, 1220
HpyF10VI GCNNNNNNNGC 2 cut(s) 34, 1106
HpyF3I CTNAG 2 cut(s) 264, 318
Hsp92II CATG 4 cut(s) 129, 463, 1004, 1287
Kzo9I GATC 5 cut(s) 343, 913, 958, 967, 1033
LmnI GCTCC 2 cut(s) 978, 1282
Lsp1109I GCAGC 2 cut(s) 12, 968
MaeI CTAG 3 cut(s) 218, 725, 1020
MaeIII GTNAC 2 cut(s) 113, 392
MalI GATC 5 cut(s) 345, 915, 960, 969, 1035
MboI GATC 5 cut(s) 343, 913, 958, 967, 1033
MboII GAAGA 6 cut(s) 419, 492, 569, 708, 1022, 1226
MfeI CAATTG 1 cut(s) 273
MflI RGATCY 1 cut(s) 1033
MhlI GDGCHC 1 cut(s) 272
MluCI AATT 6 cut(s) 63, 273, 991, 1190, 1247, 1261
MlyI GAGTC 1 cut(s) 668
MmeI TCCRAC 1 cut(s) 1160
MnlI CCTC 9 cut(s) 55, 70, 133, 327, 482, 524, 709, 935, 1206
MseI TTAA 1 cut(s) 1094
MslI CAYNNNNRTG 1 cut(s) 1005
MspI CCGG 3 cut(s) 745, 1080, 1274
MspR9I CCNGG 3 cut(s) 468, 627, 1080
MunI CAATTG 1 cut(s) 273
Mva1269I GAATGC 1 cut(s) 1234
MvaI CCWGG 2 cut(s) 468, 627
MwoI GCNNNNNNNGC 2 cut(s) 34, 1106
NciI CCSGG 1 cut(s) 1080
NdeI CATATG 1 cut(s) 1204
NdeII GATC 5 cut(s) 343, 913, 958, 967, 1033
NlaIII CATG 4 cut(s) 129, 463, 1004, 1287
NlaIV GGNNCC 1 cut(s) 1278
NmuCI GTSAC 1 cut(s) 113
PceI AGGCCT 1 cut(s) 256
PctI GAATGC 1 cut(s) 1234
PfeI GAWTC 5 cut(s) 445, 565, 596, 699, 863
Pfl23II CGTACG 1 cut(s) 972
PflMI CCANNNNNTGG 1 cut(s) 234
PkrI GCNGC 2 cut(s) 27, 983
PleI GAGTC 1 cut(s) 667
PpsI GAGTC 1 cut(s) 667
PshAI GACNNNNGTC 1 cut(s) 1221
Psp6I CCWGG 2 cut(s) 466, 625
PspGI CCWGG 2 cut(s) 466, 625
PspLI CGTACG 1 cut(s) 972
PspN4I GGNNCC 1 cut(s) 1278
PstI CTGCAG 2 cut(s) 112, 1111
PstNI CAGNNNCTG 1 cut(s) 897
PsuI RGATCY 1 cut(s) 1033
RsaI GTAC 2 cut(s) 887, 974
RsaNI GTAC 2 cut(s) 886, 973
RseI CAYNNNNRTG 1 cut(s) 1005
SalI GTCGAC 1 cut(s) 1287
SaqAI TTAA 1 cut(s) 1094
SatI GCNGC 2 cut(s) 26, 982
Sau3AI GATC 5 cut(s) 343, 913, 958, 967, 1033
SchI GAGTC 1 cut(s) 668
ScrFI CCNGG 3 cut(s) 468, 627, 1080
SduI GDGCHC 1 cut(s) 272
SfcI CTRYAG 2 cut(s) 108, 1107
SmiMI CAYNNNNRTG 1 cut(s) 1005
Sse9I AATT 6 cut(s) 63, 273, 991, 1190, 1247, 1261
SseBI AGGCCT 1 cut(s) 256
SsiI CCGC 2 cut(s) 872, 1165
SspI AATATT 3 cut(s) 477, 782, 1127
SspMI CTAG 3 cut(s) 218, 725, 1020
StuI AGGCCT 1 cut(s) 256
StyD4I CCNGG 3 cut(s) 466, 625, 1078
StyI CCWWGG 1 cut(s) 612
TaaI ACNGT 5 cut(s) 19, 622, 811, 893, 1220
TaqI TCGA 2 cut(s) 505, 1288
TasI AATT 6 cut(s) 63, 273, 991, 1190, 1247, 1261
TfiI GAWTC 5 cut(s) 445, 565, 596, 699, 863
Tru1I TTAA 1 cut(s) 1094
Tru9I TTAA 1 cut(s) 1094
TscAI CASTG 3 cut(s) 90, 301, 814
TseFI GTSAC 1 cut(s) 113
TseI GCWGC 2 cut(s) 25, 981
Tsp45I GTSAC 1 cut(s) 113
TspDTI ATGAA 3 cut(s) 395, 1185, 1231
TspGWI ACGGA 1 cut(s) 960
TspRI CASTG 3 cut(s) 90, 301, 814
Van91I CCANNNNNTGG 1 cut(s) 234
XapI RAATTY 2 cut(s) 991, 1247
XcmI CCANNNNNNNNNTGG 1 cut(s) 1065
XmiI GTMKAC 1 cut(s) 1288
XspI CTAG 3 cut(s) 218, 725, 1020
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.