FvH4_2g06190

PLAC8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0009650
Physical Location & Seq
Reverse (-)
5137915 .. 5140244
2330 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g06190.t1

Sequence Viewer

Length: 567 bp
ATGTATCCCGCAGCTCCTGACCATGAGAAATATGCAGGTCATGATCATGATCACCATATTCCAGCTGGTCAGGTTCCAGCTATGAGTCCCCATGGTGTACCAAGAGTATATGCTCCTCCATACGTAGTCAATACGGTGCGGTCTACTCGTCATGTAGGAGGGGAGTGGTCGACTGGTCTTTGCCATTGTTGTGATGATCCTGCAAACTGTCTGATCACTTTCTTTTGCCCCTGCATCACATTCGGCCAGATTGCTGAGATAGTGGAGCAAGGTTCCTCATCTTGTGCTCAACAAGGAACCTGTTATGGGATTCTTCTTGCAACTACTGGTCTAGCTTGCTTGTATTCGTGTTTCTTTCGGTCGAGATTGAGAGGTCAGTACGACTTGGAAGAATCACCTTGTGTGGATTGTCTAGTTCATTTCTGCTGTGCTACTTGTGCACTTTGTCAAGAATACAGAGAGCTCAAGAATCGTGGTTTTGATATGGGAATAGGTTGGGAAGCCAACATGGAGAGACAGAGGCGAGGAGTTACTCAAGCTCCAGTGGCACCAGGCATGACAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0008285 GO:0042127 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0065007
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

189

Amino Acids

20.71

Weight (kDa)

6.11

Isoelectric Point (pI)

60.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 56 - 154 4.1e-32 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 547
AccI GTMKAC 2 cut(s) 143, 170
AciI CCGC 2 cut(s) 9, 139
AclWI GGATC 1 cut(s) 191
AcoI YGGCCR 1 cut(s) 244
AdeI CACNNNGTG 1 cut(s) 401
AfaI GTAC 2 cut(s) 99, 380
AfiI CCNNNNNNNGG 1 cut(s) 306
AjnI CCWGG 1 cut(s) 550
AluBI AGCT 6 cut(s) 14, 65, 80, 335, 463, 539
AluI AGCT 6 cut(s) 14, 65, 80, 335, 463, 539
Alw21I GWGCWC 3 cut(s) 289, 442, 465
Alw26I GTCTC 1 cut(s) 508
Alw44I GTGCAC 1 cut(s) 438
AlwI GGATC 1 cut(s) 191
AlwNI CAGNNNCTG 1 cut(s) 17
AoxI GGCC 1 cut(s) 244
ApaLI GTGCAC 1 cut(s) 438
ApeKI GCWGC 1 cut(s) 11
AsuHPI GGTGA 2 cut(s) 44, 387
BaeGI GKGCMC 1 cut(s) 442
BanI GGYRCC 1 cut(s) 547
BanII GRGCYC 1 cut(s) 465
Bbv12I GWGCWC 3 cut(s) 289, 442, 465
BbvI GCAGC 1 cut(s) 23
BcgI CGANNNNNNTGC 2 cut(s) 223, 257
BciT130I CCWGG 1 cut(s) 552
BciVI GTATCC 1 cut(s) 15
BclI TGATCA 3 cut(s) 43, 49, 213
BcoDI GTCTC 1 cut(s) 508
BfaI CTAG 2 cut(s) 332, 413
BfuAI ACCTGC 1 cut(s) 26
BfuI GTATCC 1 cut(s) 15
BisI GCNGC 1 cut(s) 12
BlsI GCNGC 1 cut(s) 13
Bme1390I CCNGG 1 cut(s) 552
BmiI GGNNCC 4 cut(s) 75, 274, 298, 549
BmrFI CCNGG 1 cut(s) 552
BmsI GCATC 1 cut(s) 243
BpmI CTGGAG 1 cut(s) 525
BpuEI CTTGAG 2 cut(s) 449, 519
BsaAI YACGTR 1 cut(s) 124
BsaBI GATNNNNATC 1 cut(s) 48
BsaJI CCNNGG 1 cut(s) 91
BsaXI ACNNNNNCTCC 2 cut(s) 523, 553
Bsc4I CCNNNNNNNGG 1 cut(s) 306
Bse1I ACTGG 3 cut(s) 178, 331, 542
Bse8I GATNNNNATC 1 cut(s) 48
BseBI CCWGG 1 cut(s) 552
BseDI CCNNGG 1 cut(s) 91
BseJI GATNNNNATC 1 cut(s) 48
BseLI CCNNNNNNNGG 1 cut(s) 306
BseMII CTCAG 1 cut(s) 246
BseNI ACTGG 3 cut(s) 178, 331, 542
BseRI GAGGAG 2 cut(s) 105, 540
BseSI GKGCMC 1 cut(s) 442
BseXI GCAGC 1 cut(s) 23
Bsh1285I CGRYCG 1 cut(s) 362
BshFI GGCC 1 cut(s) 246
BshNI GGYRCC 1 cut(s) 547
BsiEI CGRYCG 1 cut(s) 362
BsiHKAI GWGCWC 3 cut(s) 289, 442, 465
BslFI GGGAC 1 cut(s) 72
BslI CCNNNNNNNGG 1 cut(s) 306
BsmAI GTCTC 1 cut(s) 508
BsmFI GGGAC 1 cut(s) 72
BsnI GGCC 1 cut(s) 246
Bsp1286I GDGCHC 3 cut(s) 289, 442, 465
Bsp143I GATC 4 cut(s) 43, 49, 196, 213
Bsp19I CCATGG 1 cut(s) 91
BspACI CCGC 2 cut(s) 9, 139
BspANI GGCC 1 cut(s) 246
BspCNI CTCAG 1 cut(s) 247
BspHI TCATGA 2 cut(s) 40, 46
BspLI GGNNCC 4 cut(s) 75, 274, 298, 549
BspMI ACCTGC 1 cut(s) 26
BspPI GGATC 1 cut(s) 191
BspT107I GGYRCC 1 cut(s) 547
BsrI ACTGG 3 cut(s) 178, 331, 542
BssECI CCNNGG 1 cut(s) 91
BssMI GATC 4 cut(s) 43, 49, 196, 213
BssT1I CCWWGG 1 cut(s) 91
Bst2UI CCWGG 1 cut(s) 552
Bst4CI ACNGT 2 cut(s) 136, 209
BstBAI YACGTR 1 cut(s) 124
BstC8I GCNNGC 1 cut(s) 337
BstDEI CTNAG 1 cut(s) 255
BstDSI CCRYGG 1 cut(s) 91
BstKTI GATC 4 cut(s) 46, 52, 199, 216
BstMAI GTCTC 1 cut(s) 508
BstMBI GATC 4 cut(s) 43, 49, 196, 213
BstMCI CGRYCG 1 cut(s) 362
BstMWI GCNNNNNNNGC 2 cut(s) 437, 545
BstNI CCWGG 1 cut(s) 552
BstSCI CCNGG 1 cut(s) 550
BstSLI GKGCMC 1 cut(s) 442
BstSNI TACGTA 1 cut(s) 124
BstV1I GCAGC 1 cut(s) 23
BsuI GTATCC 1 cut(s) 15
BsuRI GGCC 1 cut(s) 246
BtgI CCRYGG 1 cut(s) 91
BtsIMutI CAGTG 1 cut(s) 549
BveI ACCTGC 1 cut(s) 26
Cac8I GCNNGC 1 cut(s) 337
CaiI CAGNNNCTG 1 cut(s) 17
CciI TCATGA 2 cut(s) 40, 46
Csp6I GTAC 2 cut(s) 98, 379
CspCI CAANNNNNGTGG 2 cut(s) 454, 489
CviAII CATG 7 cut(s) 23, 41, 47, 92, 152, 508, 556
CviJI RGCY 8 cut(s) 14, 65, 80, 246, 335, 463, 503, 539
CviKI_1 RGCY 8 cut(s) 14, 65, 80, 246, 335, 463, 503, 539
CviQI GTAC 2 cut(s) 98, 379
DdeI CTNAG 1 cut(s) 255
DpnI GATC 4 cut(s) 45, 51, 198, 215
DpnII GATC 4 cut(s) 43, 49, 196, 213
DraIII CACNNNGTG 1 cut(s) 401
EaeI YGGCCR 1 cut(s) 244
Ecl136II GAGCTC 1 cut(s) 463
Eco105I TACGTA 1 cut(s) 124
Eco130I CCWWGG 1 cut(s) 91
Eco24I GRGCYC 1 cut(s) 465
Eco53kI GAGCTC 1 cut(s) 463
EcoICRI GAGCTC 1 cut(s) 463
EcoRII CCWGG 1 cut(s) 550
EcoT14I CCWWGG 1 cut(s) 91
EcoT38I GRGCYC 1 cut(s) 465
ErhI CCWWGG 1 cut(s) 91
FaeI CATG 7 cut(s) 26, 44, 50, 95, 155, 511, 559
FaqI GGGAC 1 cut(s) 72
FatI CATG 7 cut(s) 22, 40, 46, 91, 151, 507, 555
FauI CCCGC 1 cut(s) 16
FbaI TGATCA 3 cut(s) 43, 49, 213
FblI GTMKAC 2 cut(s) 143, 170
Fnu4HI GCNGC 1 cut(s) 12
FriOI GRGCYC 1 cut(s) 465
Fsp4HI GCNGC 1 cut(s) 12
FspBI CTAG 2 cut(s) 332, 413
GluI GCNGC 1 cut(s) 12
GsuI CTGGAG 1 cut(s) 525
HaeIII GGCC 1 cut(s) 246
Hin1II CATG 7 cut(s) 26, 44, 50, 95, 155, 511, 559
HincII GTYRAC 1 cut(s) 171
HindII GTYRAC 1 cut(s) 171
HinfI GANTC 4 cut(s) 85, 310, 392, 469
HphI GGTGA 2 cut(s) 44, 387
Hpy166II GTNNAC 4 cut(s) 98, 144, 171, 440
Hpy188I TCNGA 1 cut(s) 213
Hpy188III TCNNGA 6 cut(s) 17, 41, 47, 363, 449, 466
Hpy8I GTNNAC 4 cut(s) 98, 144, 171, 440
HpyCH4III ACNGT 2 cut(s) 136, 209
HpyCH4IV ACGT 1 cut(s) 123
HpyCH4V TGCA 5 cut(s) 35, 203, 234, 320, 440
HpyF10VI GCNNNNNNNGC 2 cut(s) 437, 545
HpyF3I CTNAG 1 cut(s) 255
HpySE526I ACGT 1 cut(s) 123
Hsp92II CATG 7 cut(s) 26, 44, 50, 95, 155, 511, 559
Ksp22I TGATCA 3 cut(s) 43, 49, 213
Kzo9I GATC 4 cut(s) 43, 49, 196, 213
LmnI GCTCC 4 cut(s) 19, 118, 265, 544
Lsp1109I GCAGC 1 cut(s) 23
LweI GCATC 1 cut(s) 243
MaeI CTAG 2 cut(s) 332, 413
MaeII ACGT 1 cut(s) 123
MaeIII GTNAC 1 cut(s) 529
MalI GATC 4 cut(s) 45, 51, 198, 215
MboI GATC 4 cut(s) 43, 49, 196, 213
MboII GAAGA 2 cut(s) 305, 401
MhlI GDGCHC 3 cut(s) 289, 442, 465
MlyI GAGTC 1 cut(s) 94
MnlI CCTC 6 cut(s) 126, 152, 286, 365, 513, 518
MslI CAYNNNNRTG 2 cut(s) 45, 189
MspA1I CMGCKG 1 cut(s) 65
MspR9I CCNGG 1 cut(s) 552
MvaI CCWGG 1 cut(s) 552
MwoI GCNNNNNNNGC 2 cut(s) 437, 545
NcoI CCATGG 1 cut(s) 91
NdeII GATC 4 cut(s) 43, 49, 196, 213
NlaIII CATG 7 cut(s) 26, 44, 50, 95, 155, 511, 559
NlaIV GGNNCC 4 cut(s) 75, 274, 298, 549
PagI TCATGA 2 cut(s) 40, 46
PfeI GAWTC 3 cut(s) 310, 392, 469
PkrI GCNGC 1 cut(s) 13
PleI GAGTC 1 cut(s) 93
PpsI GAGTC 1 cut(s) 93
Ppu21I YACGTR 1 cut(s) 124
Psp124BI GAGCTC 1 cut(s) 465
Psp6I CCWGG 1 cut(s) 550
PspGI CCWGG 1 cut(s) 550
PspN4I GGNNCC 4 cut(s) 75, 274, 298, 549
PstNI CAGNNNCTG 1 cut(s) 17
PvuII CAGCTG 1 cut(s) 65
RsaI GTAC 2 cut(s) 99, 380
RsaNI GTAC 2 cut(s) 98, 379
RseI CAYNNNNRTG 2 cut(s) 45, 189
SacI GAGCTC 1 cut(s) 465
SalI GTCGAC 1 cut(s) 169
SatI GCNGC 1 cut(s) 12
Sau3AI GATC 4 cut(s) 43, 49, 196, 213
SchI GAGTC 1 cut(s) 94
ScrFI CCNGG 1 cut(s) 552
SduI GDGCHC 3 cut(s) 289, 442, 465
SfaNI GCATC 1 cut(s) 243
SmiMI CAYNNNNRTG 2 cut(s) 45, 189
SmlI CTYRAG 2 cut(s) 464, 534
SmoI CTYRAG 2 cut(s) 464, 534
SnaBI TACGTA 1 cut(s) 124
SsiI CCGC 2 cut(s) 9, 139
SspMI CTAG 2 cut(s) 332, 413
SstI GAGCTC 1 cut(s) 465
StyD4I CCNGG 1 cut(s) 550
StyI CCWWGG 1 cut(s) 91
TaaI ACNGT 2 cut(s) 136, 209
TaiI ACGT 1 cut(s) 126
TaqI TCGA 2 cut(s) 170, 362
TaqII GACCGA 1 cut(s) 348
TfiI GAWTC 3 cut(s) 310, 392, 469
TscAI CASTG 1 cut(s) 549
TseI GCWGC 1 cut(s) 11
TspDTI ATGAA 1 cut(s) 407
TspRI CASTG 1 cut(s) 549
VneI GTGCAC 1 cut(s) 438
XcmI CCANNNNNNNNNTGG 1 cut(s) 62
XmiI GTMKAC 2 cut(s) 143, 170
XspI CTAG 2 cut(s) 332, 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.