RchiOBHm_Chr6g0257021

PLAC8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0009650
Physical Location & Seq
Forward (+)
12297399 .. 12300255
2857 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23055

Sequence Viewer

Length: 564 bp
ATGTATCCTACAGCTCCTGACCATGAGAAATATGCAGGTGATCATGATCACCATATTCCAGGTCATGTTCCAGCTTTGAGTCCTCATGGTGCAGCAAGAGTCTATGCTCCTCCATATGTAGTAAGTACGGTGCGGTCTGCTCGTCATGTAGGAGGGGAGTGGTCAAGTGGTCTTTGCCATTGTTGTGATGATCCTGCAAATTGTCTGATCACTTTATTTTGCCCTTGCATCACATTCGGCCAGATAGCTGAGATAGTGGATCAAGGTTCCTCGTCTTGTGCTCAAAAGGGAACTTGTTATGGGATTCTTCTTTCAACTACTGGTCTTGCTTGCTTGTACTCGTGTTTCTATCGGTCGAAGTTGAGAGGTCAGTACGACTTGGAAGAAGCACCTTGTGTGGATTGCCTAGTTCATTTCTGCTGTGCTACTTGTGCACTTTGTCAAGAGTACAGAGAGCTCAGGAGTCGTGGTTTTGATATGGGAATCGGTTGGGAAGCTAACTTGGACAGACAAAGGCGAGGAGTTACTCAAGCTCCGATTGTGGCACCAGGCATGACAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0008285 GO:0042127 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0065007
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

187

Amino Acids

20.42

Weight (kDa)

6.28

Isoelectric Point (pI)

53.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 54 - 152 2.8e-32 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 26
Acc36I ACCTGC 1 cut(s) 26
AccB1I GGYRCC 1 cut(s) 544
AciI CCGC 1 cut(s) 133
AclWI GGATC 2 cut(s) 185, 267
AcoI YGGCCR 1 cut(s) 238
AdeI CACNNNGTG 1 cut(s) 395
AfaI GTAC 4 cut(s) 127, 338, 374, 449
AgsI TTSAA 1 cut(s) 315
AjnI CCWGG 2 cut(s) 58, 547
AluBI AGCT 6 cut(s) 14, 74, 248, 457, 497, 533
AluI AGCT 6 cut(s) 14, 74, 248, 457, 497, 533
Alw21I GWGCWC 3 cut(s) 283, 436, 459
Alw44I GTGCAC 1 cut(s) 432
AlwI GGATC 2 cut(s) 185, 267
AlwNI CAGNNNCTG 1 cut(s) 17
AoxI GGCC 1 cut(s) 238
ApaLI GTGCAC 1 cut(s) 432
ApeKI GCWGC 1 cut(s) 92
AsuHPI GGTGA 2 cut(s) 41, 50
BaeGI GKGCMC 1 cut(s) 436
BanI GGYRCC 1 cut(s) 544
BanII GRGCYC 1 cut(s) 459
BauI CACGAG 1 cut(s) 340
Bbv12I GWGCWC 3 cut(s) 283, 436, 459
BbvI GCAGC 1 cut(s) 104
BcgI CGANNNNNNTGC 2 cut(s) 217, 251
BciT130I CCWGG 2 cut(s) 60, 549
BciVI GTATCC 1 cut(s) 15
BclI TGATCA 3 cut(s) 40, 46, 207
BfaI CTAG 1 cut(s) 407
BfmI CTRYAG 1 cut(s) 9
BfuAI ACCTGC 1 cut(s) 26
BfuI GTATCC 1 cut(s) 15
BisI GCNGC 1 cut(s) 93
BlsI GCNGC 1 cut(s) 94
Bme1390I CCNGG 2 cut(s) 60, 549
BmiI GGNNCC 2 cut(s) 268, 546
BmrFI CCNGG 2 cut(s) 60, 549
BmsI GCATC 1 cut(s) 237
Bpu10I CCTNAGC 1 cut(s) 458
BpuEI CTTGAG 1 cut(s) 513
BsaBI GATNNNNATC 1 cut(s) 45
BsaXI ACNNNNNCTCC 4 cut(s) 454, 484, 517, 547
Bse1I ACTGG 1 cut(s) 325
Bse8I GATNNNNATC 1 cut(s) 45
BseBI CCWGG 2 cut(s) 60, 549
BseJI GATNNNNATC 1 cut(s) 45
BseMII CTCAG 2 cut(s) 240, 472
BseNI ACTGG 1 cut(s) 325
BseRI GAGGAG 2 cut(s) 99, 534
BseSI GKGCMC 1 cut(s) 436
BseXI GCAGC 1 cut(s) 104
BsgI GTGCAG 1 cut(s) 111
Bsh1285I CGRYCG 1 cut(s) 356
BshFI GGCC 1 cut(s) 240
BshNI GGYRCC 1 cut(s) 544
BsiEI CGRYCG 1 cut(s) 356
BsiHKAI GWGCWC 3 cut(s) 283, 436, 459
BsnI GGCC 1 cut(s) 240
Bsp1286I GDGCHC 3 cut(s) 283, 436, 459
Bsp143I GATC 5 cut(s) 40, 46, 190, 207, 259
BspACI CCGC 1 cut(s) 133
BspANI GGCC 1 cut(s) 240
BspCNI CTCAG 2 cut(s) 241, 471
BspHI TCATGA 1 cut(s) 43
BspLI GGNNCC 2 cut(s) 268, 546
BspMI ACCTGC 1 cut(s) 26
BspPI GGATC 2 cut(s) 185, 267
BspT107I GGYRCC 1 cut(s) 544
BsrI ACTGG 1 cut(s) 325
BssMI GATC 5 cut(s) 40, 46, 190, 207, 259
BssSI CACGAG 1 cut(s) 340
Bst2BI CACGAG 1 cut(s) 340
Bst2UI CCWGG 2 cut(s) 60, 549
Bst4CI ACNGT 1 cut(s) 130
BstC8I GCNNGC 1 cut(s) 331
BstDEI CTNAG 2 cut(s) 249, 458
BstKTI GATC 5 cut(s) 43, 49, 193, 210, 262
BstMBI GATC 5 cut(s) 40, 46, 190, 207, 259
BstMCI CGRYCG 1 cut(s) 356
BstMWI GCNNNNNNNGC 1 cut(s) 431
BstNI CCWGG 2 cut(s) 60, 549
BstSCI CCNGG 2 cut(s) 58, 547
BstSFI CTRYAG 1 cut(s) 9
BstSLI GKGCMC 1 cut(s) 436
BstV1I GCAGC 1 cut(s) 104
BsuI GTATCC 1 cut(s) 15
BsuRI GGCC 1 cut(s) 240
BveI ACCTGC 1 cut(s) 26
Cac8I GCNNGC 1 cut(s) 331
CaiI CAGNNNCTG 1 cut(s) 17
CciI TCATGA 1 cut(s) 43
Csp6I GTAC 4 cut(s) 126, 337, 373, 448
CviAII CATG 6 cut(s) 23, 44, 65, 86, 146, 553
CviJI RGCY 7 cut(s) 14, 74, 240, 248, 457, 497, 533
CviKI_1 RGCY 7 cut(s) 14, 74, 240, 248, 457, 497, 533
CviQI GTAC 4 cut(s) 126, 337, 373, 448
DdeI CTNAG 2 cut(s) 249, 458
DpnI GATC 5 cut(s) 42, 48, 192, 209, 261
DpnII GATC 5 cut(s) 40, 46, 190, 207, 259
DraIII CACNNNGTG 1 cut(s) 395
EaeI YGGCCR 1 cut(s) 238
Ecl136II GAGCTC 1 cut(s) 457
Eco24I GRGCYC 1 cut(s) 459
Eco53kI GAGCTC 1 cut(s) 457
EcoICRI GAGCTC 1 cut(s) 457
EcoRII CCWGG 2 cut(s) 58, 547
EcoT38I GRGCYC 1 cut(s) 459
FaeI CATG 6 cut(s) 26, 47, 68, 89, 149, 556
FatI CATG 6 cut(s) 22, 43, 64, 85, 145, 552
FauNDI CATATG 1 cut(s) 115
FbaI TGATCA 3 cut(s) 40, 46, 207
Fnu4HI GCNGC 1 cut(s) 93
FriOI GRGCYC 1 cut(s) 459
Fsp4HI GCNGC 1 cut(s) 93
FspBI CTAG 1 cut(s) 407
GluI GCNGC 1 cut(s) 93
HaeIII GGCC 1 cut(s) 240
Hin1II CATG 6 cut(s) 26, 47, 68, 89, 149, 556
HinfI GANTC 5 cut(s) 79, 99, 304, 463, 483
HphI GGTGA 2 cut(s) 41, 50
Hpy166II GTNNAC 1 cut(s) 434
Hpy188I TCNGA 2 cut(s) 207, 537
Hpy188III TCNNGA 4 cut(s) 17, 44, 443, 460
Hpy8I GTNNAC 1 cut(s) 434
HpyCH4III ACNGT 1 cut(s) 130
HpyCH4V TGCA 5 cut(s) 35, 92, 197, 228, 434
HpyF10VI GCNNNNNNNGC 1 cut(s) 431
HpyF3I CTNAG 2 cut(s) 249, 458
Hsp92II CATG 6 cut(s) 26, 47, 68, 89, 149, 556
Ksp22I TGATCA 3 cut(s) 40, 46, 207
Kzo9I GATC 5 cut(s) 40, 46, 190, 207, 259
LmnI GCTCC 3 cut(s) 19, 112, 538
Lsp1109I GCAGC 1 cut(s) 104
LweI GCATC 1 cut(s) 237
MaeI CTAG 1 cut(s) 407
MaeIII GTNAC 1 cut(s) 523
MalI GATC 5 cut(s) 42, 48, 192, 209, 261
MboI GATC 5 cut(s) 40, 46, 190, 207, 259
MboII GAAGA 2 cut(s) 299, 395
MhlI GDGCHC 3 cut(s) 283, 436, 459
MluCI AATT 1 cut(s) 199
MlyI GAGTC 3 cut(s) 88, 108, 472
MnlI CCTC 6 cut(s) 93, 120, 146, 280, 359, 512
MslI CAYNNNNRTG 1 cut(s) 183
MspR9I CCNGG 2 cut(s) 60, 549
MvaI CCWGG 2 cut(s) 60, 549
MwoI GCNNNNNNNGC 1 cut(s) 431
NdeI CATATG 1 cut(s) 115
NdeII GATC 5 cut(s) 40, 46, 190, 207, 259
NlaIII CATG 6 cut(s) 26, 47, 68, 89, 149, 556
NlaIV GGNNCC 2 cut(s) 268, 546
PagI TCATGA 1 cut(s) 43
PaqCI CACCTGC 1 cut(s) 26
PfeI GAWTC 2 cut(s) 304, 483
PkrI GCNGC 1 cut(s) 94
PleI GAGTC 3 cut(s) 87, 107, 471
PpsI GAGTC 3 cut(s) 87, 107, 471
Psp124BI GAGCTC 1 cut(s) 459
Psp6I CCWGG 2 cut(s) 58, 547
PspGI CCWGG 2 cut(s) 58, 547
PspN4I GGNNCC 2 cut(s) 268, 546
PstNI CAGNNNCTG 1 cut(s) 17
RsaI GTAC 4 cut(s) 127, 338, 374, 449
RsaNI GTAC 4 cut(s) 126, 337, 373, 448
RseI CAYNNNNRTG 1 cut(s) 183
SacI GAGCTC 1 cut(s) 459
SatI GCNGC 1 cut(s) 93
Sau3AI GATC 5 cut(s) 40, 46, 190, 207, 259
SchI GAGTC 3 cut(s) 88, 108, 472
ScrFI CCNGG 2 cut(s) 60, 549
SduI GDGCHC 3 cut(s) 283, 436, 459
SfaNI GCATC 1 cut(s) 237
SfcI CTRYAG 1 cut(s) 9
SmiMI CAYNNNNRTG 1 cut(s) 183
SmlI CTYRAG 1 cut(s) 528
SmoI CTYRAG 1 cut(s) 528
Sse9I AATT 1 cut(s) 199
SsiI CCGC 1 cut(s) 133
SspMI CTAG 1 cut(s) 407
SstI GAGCTC 1 cut(s) 459
StyD4I CCNGG 2 cut(s) 58, 547
TaaI ACNGT 1 cut(s) 130
TaqI TCGA 1 cut(s) 356
TaqII GACCGA 1 cut(s) 342
TasI AATT 1 cut(s) 199
TatI WGTACW 2 cut(s) 336, 447
TfiI GAWTC 2 cut(s) 304, 483
TseI GCWGC 1 cut(s) 92
TspDTI ATGAA 1 cut(s) 401
VneI GTGCAC 1 cut(s) 432
XspI CTAG 1 cut(s) 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.