MD00G1133500.v1.1

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0009650
Physical Location & Seq
Reverse (-)
28970411 .. 28972076
1666 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1133500.v1.1.491

Sequence Viewer

Length: 642 bp
ATGTATTTACTCGTACTTCATCGTATAAAAGCTGCTTCATCTGCCGTTGCTATCCATGAGCATGACAATTCTGCACGTCAAACCAAAACATGCATAGTGCGGTCTAGTGCAGATTCCAATAACATCGATCGCATATATATATATATATATGTGCATGTGTGTGTATGTATATGCCCCAACGGTGTGCAAAAAAAGATGTCGGGTGGATGGACCACTGGCCTGCTTGACTGCTTCAGTGACTGCAGTGTCTGCTGCTTGGGATTTTGGTGTCCTTGCGTTGTTGCTGGGCGTGTTGCAGAGATTGTCTCCAAAGGACATACCAGTTGTTTTCAGCAGGGATGTTTGTATGCACTAATCAATTCCTATACTCACTTAGCATTCGGCATTCCTTGCGGTTTCTGCATAACTTGCGGCTTTCGAACCAAACTGAGGGAGCAGTACATGTTGGAGAAAAAACCTTGCAACGATTGCTGCGTTCATTTCTTCTGCCACTCCTGTGCCTTGTGCCAAGAATACCGTGAGCTCGAGAACCGTGGTTTCAATGTTACCATTGGCTGGGAACAAAATGCGTCAAAGAATCCTGGAGTGGTGACAGCACCAGCTGTGCAGGGTGGCATGAATCGTCAGACTGATTACAAATAG

Protein Analysis

214

Amino Acids

23.7

Weight (kDa)

8.33

Isoelectric Point (pI)

32.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 70 - 174 4.8e-31 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 245
AccB7I CCANNNNNTGG 1 cut(s) 555
AciI CCGC 3 cut(s) 100, 393, 411
AcuI CTGAAG 1 cut(s) 217
AfaI GTAC 2 cut(s) 15, 440
AfiI CCNNNNNNNGG 2 cut(s) 429, 555
AflIII ACRYGT 1 cut(s) 441
AgsI TTSAA 1 cut(s) 541
AjiI CACGTC 1 cut(s) 77
AjnI CCWGG 1 cut(s) 580
AleI CACNNNNGTG 1 cut(s) 495
AluBI AGCT 3 cut(s) 32, 523, 602
AluI AGCT 3 cut(s) 32, 523, 602
Alw21I GWGCWC 1 cut(s) 525
Alw26I GTCTC 1 cut(s) 310
AlwNI CAGNNNCTG 2 cut(s) 240, 249
Ama87I CYCGRG 1 cut(s) 524
AoxI GGCC 1 cut(s) 217
ApeKI GCWGC 3 cut(s) 32, 252, 471
AspS9I GGNCC 1 cut(s) 210
AsuHPI GGTGA 1 cut(s) 601
AsuII TTCGAA 1 cut(s) 418
AvaI CYCGRG 1 cut(s) 524
AvaII GGWCC 1 cut(s) 210
BanII GRGCYC 1 cut(s) 525
Bbv12I GWGCWC 1 cut(s) 525
BbvI GCAGC 3 cut(s) 19, 239, 458
BccI CCATC 1 cut(s) 201
BceAI ACGGC 1 cut(s) 29
BciT130I CCWGG 1 cut(s) 582
BcoDI GTCTC 1 cut(s) 310
BfaI CTAG 1 cut(s) 105
BfmI CTRYAG 1 cut(s) 241
BisI GCNGC 4 cut(s) 33, 253, 412, 472
BlsI GCNGC 4 cut(s) 34, 254, 413, 473
Bme1390I CCNGG 1 cut(s) 582
Bme18I GGWCC 1 cut(s) 210
BmeT110I CYCGRG 1 cut(s) 524
BmgBI CACGTC 1 cut(s) 77
BmgT120I GGNCC 1 cut(s) 210
BmrFI CCNGG 1 cut(s) 582
BplI GAGNNNNNCTC 2 cut(s) 290, 322
BpmI CTGGAG 1 cut(s) 603
Bpu14I TTCGAA 1 cut(s) 418
Bsa29I ATCGAT 1 cut(s) 126
BsaJI CCNNGG 1 cut(s) 532
Bsc4I CCNNNNNNNGG 2 cut(s) 429, 555
Bse1I ACTGG 2 cut(s) 220, 321
BseBI CCWGG 1 cut(s) 582
BseCI ATCGAT 1 cut(s) 126
BseDI CCNNGG 1 cut(s) 532
BseGI GGATG 2 cut(s) 212, 344
BseLI CCNNNNNNNGG 2 cut(s) 429, 555
BseMII CTCAG 1 cut(s) 419
BseNI ACTGG 2 cut(s) 220, 321
BseXI GCAGC 3 cut(s) 19, 239, 458
BseYI CCCAGC 2 cut(s) 284, 555
BsgI GTGCAG 3 cut(s) 57, 129, 626
Bsh1285I CGRYCG 1 cut(s) 130
BshFI GGCC 1 cut(s) 219
BshVI ATCGAT 1 cut(s) 126
BsiEI CGRYCG 1 cut(s) 130
BsiHKAI GWGCWC 1 cut(s) 525
BsiHKCI CYCGRG 1 cut(s) 524
BslI CCNNNNNNNGG 2 cut(s) 429, 555
BsmAI GTCTC 1 cut(s) 310
BsmI GAATGC 2 cut(s) 377, 384
BsnI GGCC 1 cut(s) 219
BsoBI CYCGRG 1 cut(s) 524
Bsp119I TTCGAA 1 cut(s) 418
Bsp1286I GDGCHC 1 cut(s) 525
Bsp143I GATC 1 cut(s) 127
BspACI CCGC 3 cut(s) 100, 393, 411
BspANI GGCC 1 cut(s) 219
BspCNI CTCAG 1 cut(s) 420
BspDI ATCGAT 1 cut(s) 126
BspMAI CTGCAG 1 cut(s) 245
BspT104I TTCGAA 1 cut(s) 418
BsrI ACTGG 2 cut(s) 220, 321
BssECI CCNNGG 1 cut(s) 532
BssMI GATC 1 cut(s) 127
Bst2UI CCWGG 1 cut(s) 582
Bst4CI ACNGT 3 cut(s) 182, 518, 533
BstAPI GCANNNNNTGC 4 cut(s) 249, 390, 408, 468
BstBI TTCGAA 1 cut(s) 418
BstC8I GCNNGC 1 cut(s) 221
BstDEI CTNAG 2 cut(s) 373, 428
BstDSI CCRYGG 1 cut(s) 532
BstF5I GGATG 2 cut(s) 212, 344
BstKTI GATC 1 cut(s) 130
BstMAI GTCTC 1 cut(s) 310
BstMBI GATC 1 cut(s) 127
BstMCI CGRYCG 1 cut(s) 130
BstMWI GCNNNNNNNGC 6 cut(s) 41, 249, 390, 399, 408, 468
BstNI CCWGG 1 cut(s) 582
BstNSI RCATGY 3 cut(s) 93, 158, 445
BstSCI CCNGG 1 cut(s) 580
BstSFI CTRYAG 1 cut(s) 241
BstV1I GCAGC 3 cut(s) 19, 239, 458
Bsu15I ATCGAT 1 cut(s) 126
BsuRI GGCC 1 cut(s) 219
BsuTUI ATCGAT 1 cut(s) 126
BtgI CCRYGG 1 cut(s) 532
BtrI CACGTC 1 cut(s) 77
BtsCI GGATG 2 cut(s) 212, 344
BtsI GCAGTG 1 cut(s) 250
BtsIMutI CAGTG 3 cut(s) 213, 241, 250
Cac8I GCNNGC 1 cut(s) 221
CaiI CAGNNNCTG 2 cut(s) 240, 249
Cfr13I GGNCC 1 cut(s) 210
ClaI ATCGAT 1 cut(s) 126
CseI GACGC 1 cut(s) 558
Csp6I GTAC 2 cut(s) 14, 439
CviAII CATG 6 cut(s) 56, 62, 90, 155, 442, 616
CviJI RGCY 6 cut(s) 32, 219, 414, 523, 555, 602
CviKI_1 RGCY 6 cut(s) 32, 219, 414, 523, 555, 602
CviQI GTAC 2 cut(s) 14, 439
DdeI CTNAG 2 cut(s) 373, 428
DpnI GATC 1 cut(s) 129
DpnII GATC 1 cut(s) 127
DrdI GACNNNNNNGTC 1 cut(s) 245
DseDI GACNNNNNNGTC 1 cut(s) 245
Ecl136II GAGCTC 1 cut(s) 523
Eco24I GRGCYC 1 cut(s) 525
Eco47I GGWCC 1 cut(s) 210
Eco53kI GAGCTC 1 cut(s) 523
Eco57I CTGAAG 1 cut(s) 217
Eco88I CYCGRG 1 cut(s) 524
EcoICRI GAGCTC 1 cut(s) 523
EcoRII CCWGG 1 cut(s) 580
EcoT22I ATGCAT 1 cut(s) 95
EcoT38I GRGCYC 1 cut(s) 525
FaeI CATG 6 cut(s) 59, 65, 93, 158, 445, 619
FatI CATG 6 cut(s) 55, 61, 89, 154, 441, 615
Fnu4HI GCNGC 4 cut(s) 33, 253, 412, 472
FokI GGATG 2 cut(s) 219, 351
FriOI GRGCYC 1 cut(s) 525
Fsp4HI GCNGC 4 cut(s) 33, 253, 412, 472
FspBI CTAG 1 cut(s) 105
GluI GCNGC 4 cut(s) 33, 253, 412, 472
GsaI CCCAGC 2 cut(s) 288, 559
GsuI CTGGAG 1 cut(s) 603
HaeIII GGCC 1 cut(s) 219
HgaI GACGC 1 cut(s) 558
Hin1II CATG 6 cut(s) 59, 65, 93, 158, 445, 619
HinfI GANTC 3 cut(s) 113, 577, 619
HphI GGTGA 1 cut(s) 601
Hpy188I TCNGA 1 cut(s) 627
Hpy188III TCNNGA 1 cut(s) 526
HpyCH4III ACNGT 3 cut(s) 182, 518, 533
HpyCH4IV ACGT 1 cut(s) 76
HpyF10VI GCNNNNNNNGC 6 cut(s) 41, 249, 390, 399, 408, 468
HpyF3I CTNAG 2 cut(s) 373, 428
HpySE526I ACGT 1 cut(s) 76
Hsp92II CATG 6 cut(s) 59, 65, 93, 158, 445, 619
Kzo9I GATC 1 cut(s) 127
LmnI GCTCC 1 cut(s) 433
Lsp1109I GCAGC 3 cut(s) 19, 239, 458
MaeI CTAG 1 cut(s) 105
MaeII ACGT 1 cut(s) 76
MaeIII GTNAC 3 cut(s) 236, 544, 589
MalI GATC 1 cut(s) 129
MboI GATC 1 cut(s) 127
MboII GAAGA 1 cut(s) 475
MhlI GDGCHC 1 cut(s) 525
MluCI AATT 2 cut(s) 67, 358
MmeI TCCRAC 1 cut(s) 426
MnlI CCTC 1 cut(s) 423
Mph1103I ATGCAT 1 cut(s) 95
MslI CAYNNNNRTG 3 cut(s) 60, 159, 495
MspA1I CMGCKG 1 cut(s) 602
MspR9I CCNGG 1 cut(s) 582
Mva1269I GAATGC 2 cut(s) 377, 384
MvaI CCWGG 1 cut(s) 582
MwoI GCNNNNNNNGC 6 cut(s) 41, 249, 390, 399, 408, 468
NdeII GATC 1 cut(s) 127
NlaIII CATG 6 cut(s) 59, 65, 93, 158, 445, 619
NmuCI GTSAC 2 cut(s) 236, 589
NsiI ATGCAT 1 cut(s) 95
NspI RCATGY 3 cut(s) 93, 158, 445
NspV TTCGAA 1 cut(s) 418
OliI CACNNNNGTG 1 cut(s) 495
PaeR7I CTCGAG 1 cut(s) 524
PciI ACATGT 1 cut(s) 441
PcsI WCGNNNNNNNCGW 1 cut(s) 471
PctI GAATGC 2 cut(s) 377, 384
PfeI GAWTC 3 cut(s) 113, 577, 619
PflMI CCANNNNNTGG 1 cut(s) 555
PfoI TCCNGGA 1 cut(s) 580
PkrI GCNGC 4 cut(s) 34, 254, 413, 473
Ple19I CGATCG 1 cut(s) 130
PscI ACATGT 1 cut(s) 441
Psp124BI GAGCTC 1 cut(s) 525
Psp6I CCWGG 1 cut(s) 580
PspFI CCCAGC 2 cut(s) 284, 555
PspGI CCWGG 1 cut(s) 580
PspPI GGNCC 1 cut(s) 210
PstI CTGCAG 1 cut(s) 245
PstNI CAGNNNCTG 2 cut(s) 240, 249
PvuI CGATCG 1 cut(s) 130
PvuII CAGCTG 1 cut(s) 602
RsaI GTAC 2 cut(s) 15, 440
RsaNI GTAC 2 cut(s) 14, 439
RseI CAYNNNNRTG 3 cut(s) 60, 159, 495
SacI GAGCTC 1 cut(s) 525
SatI GCNGC 4 cut(s) 33, 253, 412, 472
Sau3AI GATC 1 cut(s) 127
Sau96I GGNCC 1 cut(s) 210
ScrFI CCNGG 1 cut(s) 582
SduI GDGCHC 1 cut(s) 525
SetI ASST 5 cut(s) 34, 79, 460, 525, 604
SfcI CTRYAG 1 cut(s) 241
Sfr274I CTCGAG 1 cut(s) 524
SfuI TTCGAA 1 cut(s) 418
SinI GGWCC 1 cut(s) 210
SlaI CTCGAG 1 cut(s) 524
SmiMI CAYNNNNRTG 3 cut(s) 60, 159, 495
SmlI CTYRAG 1 cut(s) 524
SmoI CTYRAG 1 cut(s) 524
Sse9I AATT 2 cut(s) 67, 358
SsiI CCGC 3 cut(s) 100, 393, 411
SspMI CTAG 1 cut(s) 105
SstI GAGCTC 1 cut(s) 525
StyD4I CCNGG 1 cut(s) 580
TaaI ACNGT 3 cut(s) 182, 518, 533
TaiI ACGT 1 cut(s) 79
TaqI TCGA 3 cut(s) 126, 418, 525
TasI AATT 2 cut(s) 67, 358
TatI WGTACW 1 cut(s) 438
TauI GCSGC 1 cut(s) 414
TfiI GAWTC 3 cut(s) 113, 577, 619
TscAI CASTG 3 cut(s) 220, 241, 250
TseFI GTSAC 2 cut(s) 236, 589
TseI GCWGC 3 cut(s) 32, 252, 471
Tsp45I GTSAC 2 cut(s) 236, 589
TspDTI ATGAA 4 cut(s) 8, 27, 467, 632
TspRI CASTG 3 cut(s) 220, 241, 250
Van91I CCANNNNNTGG 1 cut(s) 555
VpaK11BI GGWCC 1 cut(s) 210
XceI RCATGY 3 cut(s) 93, 158, 445
XhoI CTCGAG 1 cut(s) 524
XspI CTAG 1 cut(s) 105
Zsp2I ATGCAT 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.