MD05G1079900.v1.1

PLAC8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr05
OG0009650
Physical Location & Seq
Forward (+)
16747485 .. 16749368
1884 bp
Loading structure...
UTR
Exon/CDS
Intron
MD05G1079900.v1.1.491

Sequence Viewer

Length: 603 bp
ATGTATCCAACTCCTACAGAAACTCATGACCATGAGAAATATGCAGCCCATCACAACCATCACCATGCCCATGTTCCCAATCCAGTCTCAAGTGCACCAGGACTAAATAGTCCTGCAGGTTCAGTACCCACATATGCTCCTCCCTACATATACACTGTTCAGTCTAATTCTGTTCCTGCAGTTCCCGCACGAGGGAACTGGTCCACTAGTCTTTGCCACTGTTGTGACGATCCTGAAAATTGTATGATCACTTTCTTTTGCCCTTGCATCACGTTCGGTCAGATCGCTGAGATTGTGAGCCAAGGTTCCACACCTTGTGCTTCAGGAGGAGTGTGCTATTGTATCCTTCTTTCGACAACTGCAAATGCATGCTTGTACTCGTGCTCCTACCGCTCGAAGATGAGAGCTCAATATGACTTGGAAGAAACACCCTGTGTGGATTGCCTAGTGCACTTCTGCTGTGCTACTTGTGCACTTTGTCAAGAGTATAGAGAGCTCAAGAATCGCGGGTTCGATATGGGAATAGGTTGGGAAGCCAATATGGACAGACGAAGGCGCGGCGTTACTGCAGCTCCAACTGTTGTACCAGGCATGACAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0008150 GO:0008285 GO:0042127 GO:0048519 GO:0048523 GO:0050789 GO:0050794 GO:0065007
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

201

Amino Acids

21.89

Weight (kDa)

6.33

Isoelectric Point (pI)

56.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 67 - 165 2.8e-31 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 108
Acc36I ACCTGC 1 cut(s) 107
AccBSI CCGCTC 1 cut(s) 393
AccII CGCG 2 cut(s) 507, 558
AciI CCGC 4 cut(s) 186, 391, 507, 558
AclWI GGATC 1 cut(s) 224
AcuI CTGAAG 1 cut(s) 306
AdeI CACNNNGTG 2 cut(s) 317, 434
AfaI GTAC 3 cut(s) 126, 377, 585
AfiI CCNNNNNNNGG 2 cut(s) 191, 192
AhlI ACTAGT 1 cut(s) 206
AjnI CCWGG 2 cut(s) 97, 586
AleI CACNNNNGTG 1 cut(s) 222
AluBI AGCT 3 cut(s) 407, 496, 572
AluI AGCT 3 cut(s) 407, 496, 572
Alw21I GWGCWC 6 cut(s) 97, 386, 409, 453, 475, 498
Alw26I GTCTC 1 cut(s) 91
Alw44I GTGCAC 3 cut(s) 93, 449, 471
AlwI GGATC 1 cut(s) 224
ApaLI GTGCAC 3 cut(s) 93, 449, 471
ApeKI GCWGC 2 cut(s) 44, 569
AspLEI GCGC 1 cut(s) 558
AspS9I GGNCC 1 cut(s) 201
AsuHPI GGTGA 1 cut(s) 53
AvaII GGWCC 1 cut(s) 201
BaeGI GKGCMC 3 cut(s) 97, 453, 475
BaeI ACNNNNGTAYC 2 cut(s) 567, 600
BanII GRGCYC 2 cut(s) 409, 498
BauI CACGAG 2 cut(s) 189, 379
Bbv12I GWGCWC 6 cut(s) 97, 386, 409, 453, 475, 498
BbvI GCAGC 2 cut(s) 56, 581
BccI CCATC 2 cut(s) 57, 66
BcgI CGANNNNNNTGC 2 cut(s) 256, 290
BciT130I CCWGG 2 cut(s) 99, 588
BciVI GTATCC 2 cut(s) 15, 353
BclI TGATCA 1 cut(s) 246
BcoDI GTCTC 1 cut(s) 91
BcuI ACTAGT 1 cut(s) 206
BfaI CTAG 2 cut(s) 207, 446
BfmI CTRYAG 4 cut(s) 15, 114, 177, 567
BfuAI ACCTGC 1 cut(s) 107
BfuI GTATCC 2 cut(s) 15, 353
BisI GCNGC 3 cut(s) 45, 559, 570
BlsI GCNGC 3 cut(s) 46, 560, 571
Bme1390I CCNGG 2 cut(s) 99, 588
Bme18I GGWCC 1 cut(s) 201
BmgT120I GGNCC 1 cut(s) 201
BmiI GGNNCC 1 cut(s) 307
BmrFI CCNGG 2 cut(s) 99, 588
BmsI GCATC 1 cut(s) 276
BpuEI CTTGAG 2 cut(s) 73, 482
BsaJI CCNNGG 1 cut(s) 301
BsaXI ACNNNNNCTCC 6 cut(s) 121, 151, 368, 398, 556, 586
Bsc4I CCNNNNNNNGG 2 cut(s) 191, 192
Bse1I ACTGG 2 cut(s) 83, 203
BseBI CCWGG 2 cut(s) 99, 588
BseDI CCNNGG 1 cut(s) 301
BseLI CCNNNNNNNGG 2 cut(s) 191, 192
BseMII CTCAG 1 cut(s) 279
BseNI ACTGG 2 cut(s) 83, 203
BseRI GAGGAG 2 cut(s) 129, 342
BseSI GKGCMC 3 cut(s) 97, 453, 475
BseXI GCAGC 2 cut(s) 56, 581
Bsh1236I CGCG 2 cut(s) 507, 558
BsiHKAI GWGCWC 6 cut(s) 97, 386, 409, 453, 475, 498
BslI CCNNNNNNNGG 2 cut(s) 191, 192
BsmAI GTCTC 1 cut(s) 91
Bsp1286I GDGCHC 6 cut(s) 97, 386, 409, 453, 475, 498
Bsp143I GATC 3 cut(s) 229, 246, 282
BspACI CCGC 4 cut(s) 186, 391, 507, 558
BspCNI CTCAG 1 cut(s) 280
BspFNI CGCG 2 cut(s) 507, 558
BspHI TCATGA 1 cut(s) 25
BspLI GGNNCC 1 cut(s) 307
BspMAI CTGCAG 3 cut(s) 118, 181, 571
BspMI ACCTGC 1 cut(s) 107
BspPI GGATC 1 cut(s) 224
BsrBI CCGCTC 1 cut(s) 393
BsrI ACTGG 2 cut(s) 83, 203
BssECI CCNNGG 1 cut(s) 301
BssMI GATC 3 cut(s) 229, 246, 282
BssSI CACGAG 2 cut(s) 189, 379
BssT1I CCWWGG 1 cut(s) 301
Bst2BI CACGAG 2 cut(s) 189, 379
Bst2UI CCWGG 2 cut(s) 99, 588
Bst4CI ACNGT 3 cut(s) 157, 221, 580
BstC8I GCNNGC 1 cut(s) 370
BstDEI CTNAG 1 cut(s) 288
BstFNI CGCG 2 cut(s) 507, 558
BstHHI GCGC 1 cut(s) 558
BstKTI GATC 3 cut(s) 232, 249, 285
BstMAI GTCTC 1 cut(s) 91
BstMBI GATC 3 cut(s) 229, 246, 282
BstMWI GCNNNNNNNGC 3 cut(s) 185, 390, 470
BstNI CCWGG 2 cut(s) 99, 588
BstNSI RCATGY 1 cut(s) 372
BstSCI CCNGG 2 cut(s) 97, 586
BstSFI CTRYAG 4 cut(s) 15, 114, 177, 567
BstSLI GKGCMC 3 cut(s) 97, 453, 475
BstUI CGCG 2 cut(s) 507, 558
BstV1I GCAGC 2 cut(s) 56, 581
BsuI GTATCC 2 cut(s) 15, 353
BtsIMutI CAGTG 2 cut(s) 153, 217
BveI ACCTGC 1 cut(s) 107
Cac8I GCNNGC 1 cut(s) 370
CciI TCATGA 1 cut(s) 25
CfoI GCGC 1 cut(s) 558
Cfr13I GGNCC 1 cut(s) 201
Csp6I GTAC 3 cut(s) 125, 376, 584
CviAII CATG 6 cut(s) 26, 32, 65, 71, 369, 592
CviJI RGCY 6 cut(s) 47, 300, 407, 496, 536, 572
CviKI_1 RGCY 6 cut(s) 47, 300, 407, 496, 536, 572
CviQI GTAC 3 cut(s) 125, 376, 584
DdeI CTNAG 1 cut(s) 288
DpnI GATC 3 cut(s) 231, 248, 284
DpnII GATC 3 cut(s) 229, 246, 282
DraIII CACNNNGTG 2 cut(s) 317, 434
DrdI GACNNNNNNGTC 1 cut(s) 108
DseDI GACNNNNNNGTC 1 cut(s) 108
Ecl136II GAGCTC 2 cut(s) 407, 496
Eco130I CCWWGG 1 cut(s) 301
Eco24I GRGCYC 2 cut(s) 409, 498
Eco47I GGWCC 1 cut(s) 201
Eco53kI GAGCTC 2 cut(s) 407, 496
Eco57I CTGAAG 1 cut(s) 306
EcoICRI GAGCTC 2 cut(s) 407, 496
EcoRII CCWGG 2 cut(s) 97, 586
EcoT14I CCWWGG 1 cut(s) 301
EcoT22I ATGCAT 1 cut(s) 370
EcoT38I GRGCYC 2 cut(s) 409, 498
ErhI CCWWGG 1 cut(s) 301
FaeI CATG 6 cut(s) 29, 35, 68, 74, 372, 595
FatI CATG 6 cut(s) 25, 31, 64, 70, 368, 591
FauI CCCGC 2 cut(s) 193, 500
FauNDI CATATG 1 cut(s) 133
FbaI TGATCA 1 cut(s) 246
Fnu4HI GCNGC 3 cut(s) 45, 559, 570
FriOI GRGCYC 2 cut(s) 409, 498
Fsp4HI GCNGC 3 cut(s) 45, 559, 570
FspBI CTAG 2 cut(s) 207, 446
GlaI GCGC 1 cut(s) 557
GluI GCNGC 3 cut(s) 45, 559, 570
HhaI GCGC 1 cut(s) 558
Hin1II CATG 6 cut(s) 29, 35, 68, 74, 372, 595
Hin6I GCGC 1 cut(s) 556
HinP1I GCGC 1 cut(s) 556
HinfI GANTC 1 cut(s) 502
HphI GGTGA 1 cut(s) 53
Hpy166II GTNNAC 4 cut(s) 95, 204, 451, 473
Hpy188I TCNGA 1 cut(s) 282
Hpy188III TCNNGA 5 cut(s) 26, 233, 324, 482, 499
Hpy8I GTNNAC 4 cut(s) 95, 204, 451, 473
HpyAV CCTTC 2 cut(s) 356, 546
HpyCH4III ACNGT 3 cut(s) 157, 221, 580
HpyCH4IV ACGT 1 cut(s) 272
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 390, 470
HpyF3I CTNAG 1 cut(s) 288
HpySE526I ACGT 1 cut(s) 272
Hsp92II CATG 6 cut(s) 29, 35, 68, 74, 372, 595
HspAI GCGC 1 cut(s) 556
Ksp22I TGATCA 1 cut(s) 246
Kzo9I GATC 3 cut(s) 229, 246, 282
LmnI GCTCC 3 cut(s) 142, 389, 577
Lsp1109I GCAGC 2 cut(s) 56, 581
LweI GCATC 1 cut(s) 276
MaeI CTAG 2 cut(s) 207, 446
MaeII ACGT 1 cut(s) 272
MaeIII GTNAC 2 cut(s) 224, 562
MalI GATC 3 cut(s) 231, 248, 284
MbiI CCGCTC 1 cut(s) 393
MboI GATC 3 cut(s) 229, 246, 282
MboII GAAGA 2 cut(s) 409, 434
MhlI GDGCHC 6 cut(s) 97, 386, 409, 453, 475, 498
MluCI AATT 2 cut(s) 166, 238
MmeI TCCRAC 2 cut(s) 32, 599
MnlI CCTC 3 cut(s) 150, 185, 320
Mph1103I ATGCAT 1 cut(s) 370
MslI CAYNNNNRTG 4 cut(s) 30, 63, 69, 222
MspR9I CCNGG 2 cut(s) 99, 588
MvaI CCWGG 2 cut(s) 99, 588
MvnI CGCG 2 cut(s) 507, 558
MwoI GCNNNNNNNGC 3 cut(s) 185, 390, 470
NdeI CATATG 1 cut(s) 133
NdeII GATC 3 cut(s) 229, 246, 282
NlaIII CATG 6 cut(s) 29, 35, 68, 74, 372, 595
NlaIV GGNNCC 1 cut(s) 307
NmuCI GTSAC 1 cut(s) 224
NsiI ATGCAT 1 cut(s) 370
NspI RCATGY 1 cut(s) 372
OliI CACNNNNGTG 1 cut(s) 222
PaeI GCATGC 1 cut(s) 372
PagI TCATGA 1 cut(s) 25
PfeI GAWTC 1 cut(s) 502
PkrI GCNGC 3 cut(s) 46, 560, 571
Psp124BI GAGCTC 2 cut(s) 409, 498
Psp6I CCWGG 2 cut(s) 97, 586
PspGI CCWGG 2 cut(s) 97, 586
PspN4I GGNNCC 1 cut(s) 307
PspPI GGNCC 1 cut(s) 201
PstI CTGCAG 3 cut(s) 118, 181, 571
RsaI GTAC 3 cut(s) 126, 377, 585
RsaNI GTAC 3 cut(s) 125, 376, 584
RseI CAYNNNNRTG 4 cut(s) 30, 63, 69, 222
SacI GAGCTC 2 cut(s) 409, 498
SatI GCNGC 3 cut(s) 45, 559, 570
Sau3AI GATC 3 cut(s) 229, 246, 282
Sau96I GGNCC 1 cut(s) 201
SbfI CCTGCAGG 1 cut(s) 118
ScrFI CCNGG 2 cut(s) 99, 588
SdaI CCTGCAGG 1 cut(s) 118
SduI GDGCHC 6 cut(s) 97, 386, 409, 453, 475, 498
SetI ASST 8 cut(s) 121, 275, 307, 316, 409, 498, 529, 574
SfaNI GCATC 1 cut(s) 276
SfcI CTRYAG 4 cut(s) 15, 114, 177, 567
SinI GGWCC 1 cut(s) 201
SmiMI CAYNNNNRTG 4 cut(s) 30, 63, 69, 222
SmlI CTYRAG 2 cut(s) 88, 497
SmoI CTYRAG 2 cut(s) 88, 497
SpeI ACTAGT 1 cut(s) 206
SphI GCATGC 1 cut(s) 372
Sse8387I CCTGCAGG 1 cut(s) 118
Sse9I AATT 2 cut(s) 166, 238
SsiI CCGC 4 cut(s) 186, 391, 507, 558
SspMI CTAG 2 cut(s) 207, 446
SstI GAGCTC 2 cut(s) 409, 498
StyD4I CCNGG 2 cut(s) 97, 586
StyI CCWWGG 1 cut(s) 301
TaaI ACNGT 3 cut(s) 157, 221, 580
TaiI ACGT 1 cut(s) 275
TaqI TCGA 3 cut(s) 353, 395, 513
TaqII GACCGA 1 cut(s) 266
TasI AATT 2 cut(s) 166, 238
TatI WGTACW 1 cut(s) 375
TauI GCSGC 1 cut(s) 561
TfiI GAWTC 1 cut(s) 502
TscAI CASTG 2 cut(s) 160, 224
TseFI GTSAC 1 cut(s) 224
TseI GCWGC 2 cut(s) 44, 569
Tsp45I GTSAC 1 cut(s) 224
TspRI CASTG 2 cut(s) 160, 224
VneI GTGCAC 3 cut(s) 93, 449, 471
VpaK11BI GGWCC 1 cut(s) 201
XceI RCATGY 1 cut(s) 372
XspI CTAG 2 cut(s) 207, 446
Zsp2I ATGCAT 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.