MD10G1106000.v1.1

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0009650
Physical Location & Seq
Forward (+)
17339231 .. 17339919
689 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1106000.v1.1.491

Sequence Viewer

Length: 363 bp
ATGATGTTGAAATTGGAAGTGAACTCCAAAAGTGTGCATGCGTATGCAGGTTGTTTTCAGCAGGGATGTTTGTATGCACTAATCAATTCCTATACTCACTTAGCATTCGGCATTCCTTGCGGTTTCTGCATAACTTGCGGCTTTCGAACCAAACTGAGGGAGCAGTACATGTTGGAGAAAAAACCTTGCAACGATTGCTGCGTTCATTTCTTCTGCCACTCCTGTGCCTTGTGCCAAGAATACCGTGAGCTCGAGAACCGTGGTTTCAATGTTACCATTGGTACGTACATTCGTTGTTTCCTCCCTCCAATACAATCACTTAGATTCACCGTCTCGGTGCACCTCCTTATCCATCCTAGTTAA

Protein Analysis

121

Amino Acids

13.77

Weight (kDa)

8.47

Isoelectric Point (pI)

29.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 21 - 83 1e-16 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 38
AciI CCGC 2 cut(s) 120, 138
AfaI GTAC 3 cut(s) 167, 283, 287
AfiI CCNNNNNNNGG 1 cut(s) 156
AflIII ACRYGT 1 cut(s) 168
AgsI TTSAA 2 cut(s) 10, 268
AleI CACNNNNGTG 1 cut(s) 222
AluBI AGCT 1 cut(s) 250
AluI AGCT 1 cut(s) 250
Alw21I GWGCWC 2 cut(s) 252, 342
Alw26I GTCTC 1 cut(s) 337
Alw44I GTGCAC 1 cut(s) 338
Ama87I CYCGRG 1 cut(s) 251
ApaLI GTGCAC 1 cut(s) 338
ApeKI GCWGC 1 cut(s) 198
AsuHPI GGTGA 1 cut(s) 319
AsuII TTCGAA 1 cut(s) 145
AvaI CYCGRG 1 cut(s) 251
BaeGI GKGCMC 1 cut(s) 342
BanII GRGCYC 1 cut(s) 252
Bbv12I GWGCWC 2 cut(s) 252, 342
BbvI GCAGC 1 cut(s) 185
BccI CCATC 1 cut(s) 360
BcoDI GTCTC 1 cut(s) 337
BfaI CTAG 1 cut(s) 357
BfuAI ACCTGC 1 cut(s) 38
BisI GCNGC 2 cut(s) 139, 199
BlsI GCNGC 2 cut(s) 140, 200
BmeT110I CYCGRG 1 cut(s) 251
Bpu14I TTCGAA 1 cut(s) 145
BsaAI YACGTR 1 cut(s) 285
BsaJI CCNNGG 1 cut(s) 259
Bsc4I CCNNNNNNNGG 1 cut(s) 156
BseDI CCNNGG 1 cut(s) 259
BseGI GGATG 2 cut(s) 71, 352
BseLI CCNNNNNNNGG 1 cut(s) 156
BseMII CTCAG 1 cut(s) 146
BseSI GKGCMC 1 cut(s) 342
BseXI GCAGC 1 cut(s) 185
BsiHKAI GWGCWC 2 cut(s) 252, 342
BsiHKCI CYCGRG 1 cut(s) 251
BslI CCNNNNNNNGG 1 cut(s) 156
BsmAI GTCTC 1 cut(s) 337
BsmBI CGTCTC 1 cut(s) 337
BsmI GAATGC 2 cut(s) 104, 111
BsoBI CYCGRG 1 cut(s) 251
Bsp119I TTCGAA 1 cut(s) 145
Bsp1286I GDGCHC 2 cut(s) 252, 342
BspACI CCGC 2 cut(s) 120, 138
BspCNI CTCAG 1 cut(s) 147
BspMI ACCTGC 1 cut(s) 38
BspT104I TTCGAA 1 cut(s) 145
BssECI CCNNGG 1 cut(s) 259
Bst4CI ACNGT 3 cut(s) 245, 260, 331
BstAPI GCANNNNNTGC 3 cut(s) 117, 135, 195
BstBAI YACGTR 1 cut(s) 285
BstBI TTCGAA 1 cut(s) 145
BstC8I GCNNGC 1 cut(s) 39
BstDEI CTNAG 3 cut(s) 100, 155, 320
BstDSI CCRYGG 1 cut(s) 259
BstF5I GGATG 2 cut(s) 71, 352
BstMAI GTCTC 1 cut(s) 337
BstMWI GCNNNNNNNGC 4 cut(s) 117, 126, 135, 195
BstNSI RCATGY 2 cut(s) 41, 172
BstSLI GKGCMC 1 cut(s) 342
BstSNI TACGTA 1 cut(s) 285
BstV1I GCAGC 1 cut(s) 185
BtgI CCRYGG 1 cut(s) 259
BtsCI GGATG 2 cut(s) 71, 352
BveI ACCTGC 1 cut(s) 38
Cac8I GCNNGC 1 cut(s) 39
Csp6I GTAC 3 cut(s) 166, 282, 286
CviAII CATG 2 cut(s) 38, 169
CviJI RGCY 2 cut(s) 141, 250
CviKI_1 RGCY 2 cut(s) 141, 250
CviQI GTAC 3 cut(s) 166, 282, 286
DdeI CTNAG 3 cut(s) 100, 155, 320
Ecl136II GAGCTC 1 cut(s) 250
Eco105I TACGTA 1 cut(s) 285
Eco24I GRGCYC 1 cut(s) 252
Eco53kI GAGCTC 1 cut(s) 250
Eco88I CYCGRG 1 cut(s) 251
EcoICRI GAGCTC 1 cut(s) 250
EcoT38I GRGCYC 1 cut(s) 252
Esp3I CGTCTC 1 cut(s) 337
FaeI CATG 2 cut(s) 41, 172
FaiI YATR 6 cut(s) 39, 45, 75, 93, 131, 170
FatI CATG 2 cut(s) 37, 168
Fnu4HI GCNGC 2 cut(s) 139, 199
FokI GGATG 2 cut(s) 78, 339
FriOI GRGCYC 1 cut(s) 252
Fsp4HI GCNGC 2 cut(s) 139, 199
FspBI CTAG 1 cut(s) 357
GluI GCNGC 2 cut(s) 139, 199
Hin1II CATG 2 cut(s) 41, 172
HinfI GANTC 1 cut(s) 324
HphI GGTGA 1 cut(s) 319
Hpy166II GTNNAC 2 cut(s) 22, 340
Hpy188III TCNNGA 1 cut(s) 253
Hpy8I GTNNAC 2 cut(s) 22, 340
HpyCH4III ACNGT 3 cut(s) 245, 260, 331
HpyCH4IV ACGT 1 cut(s) 284
HpyCH4V TGCA 6 cut(s) 37, 47, 77, 129, 189, 340
HpyF10VI GCNNNNNNNGC 4 cut(s) 117, 126, 135, 195
HpyF3I CTNAG 3 cut(s) 100, 155, 320
HpySE526I ACGT 1 cut(s) 284
Hsp92II CATG 2 cut(s) 41, 172
LmnI GCTCC 1 cut(s) 160
LpnPI CCDG 3 cut(s) 33, 47, 235
Lsp1109I GCAGC 1 cut(s) 185
MaeI CTAG 1 cut(s) 357
MaeII ACGT 1 cut(s) 284
MaeIII GTNAC 1 cut(s) 271
MboII GAAGA 1 cut(s) 202
MhlI GDGCHC 2 cut(s) 252, 342
MluCI AATT 2 cut(s) 11, 85
MmeI TCCRAC 1 cut(s) 153
MnlI CCTC 4 cut(s) 150, 311, 315, 353
MseI TTAA 1 cut(s) 361
MslI CAYNNNNRTG 2 cut(s) 42, 222
Mva1269I GAATGC 2 cut(s) 104, 111
MwoI GCNNNNNNNGC 4 cut(s) 117, 126, 135, 195
NlaIII CATG 2 cut(s) 41, 172
NspI RCATGY 2 cut(s) 41, 172
NspV TTCGAA 1 cut(s) 145
OliI CACNNNNGTG 1 cut(s) 222
PaeI GCATGC 1 cut(s) 41
PaeR7I CTCGAG 1 cut(s) 251
PciI ACATGT 1 cut(s) 168
PcsI WCGNNNNNNNCGW 1 cut(s) 198
PctI GAATGC 2 cut(s) 104, 111
PfeI GAWTC 1 cut(s) 324
PkrI GCNGC 2 cut(s) 140, 200
Ppu21I YACGTR 1 cut(s) 285
PscI ACATGT 1 cut(s) 168
Psp124BI GAGCTC 1 cut(s) 252
RsaI GTAC 3 cut(s) 167, 283, 287
RsaNI GTAC 3 cut(s) 166, 282, 286
RseI CAYNNNNRTG 2 cut(s) 42, 222
SacI GAGCTC 1 cut(s) 252
SaqAI TTAA 1 cut(s) 361
SatI GCNGC 2 cut(s) 139, 199
SduI GDGCHC 2 cut(s) 252, 342
SetI ASST 5 cut(s) 52, 187, 252, 287, 345
Sfr274I CTCGAG 1 cut(s) 251
SfuI TTCGAA 1 cut(s) 145
SlaI CTCGAG 1 cut(s) 251
SmiMI CAYNNNNRTG 2 cut(s) 42, 222
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
SnaBI TACGTA 1 cut(s) 285
SphI GCATGC 1 cut(s) 41
Sse9I AATT 2 cut(s) 11, 85
SsiI CCGC 2 cut(s) 120, 138
SspMI CTAG 1 cut(s) 357
SstI GAGCTC 1 cut(s) 252
TaaI ACNGT 3 cut(s) 245, 260, 331
TaiI ACGT 1 cut(s) 287
TaqI TCGA 2 cut(s) 145, 252
TasI AATT 2 cut(s) 11, 85
TatI WGTACW 1 cut(s) 165
TauI GCSGC 1 cut(s) 141
TfiI GAWTC 1 cut(s) 324
Tru1I TTAA 1 cut(s) 361
Tru9I TTAA 1 cut(s) 361
TseI GCWGC 1 cut(s) 198
TspDTI ATGAA 1 cut(s) 194
VneI GTGCAC 1 cut(s) 338
XceI RCATGY 2 cut(s) 41, 172
XhoI CTCGAG 1 cut(s) 251
XspI CTAG 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.