MD00G1133300.v1.1

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0009650
Physical Location & Seq
Reverse (-)
28955334 .. 28956697
1364 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1133300.v1.1.491

Sequence Viewer

Length: 471 bp
ATGGTGTGTGTATGTATATGCCCCAACGGTGTGGAAAAAAAGATGTCGGGTGGATGGACCACTGGCCTGCTTGACTGCTTCAGTGACTGCAGTGTCTGCTGCTTGGGATTTTGGTGTCCTTGCGTTGTTGCTGGGCGTGTTGCAGAGATTGTCTCCAAAGGACAAACCAGTTGTTTTTGGCATGGATGTTTATATGCAGCAATTAATTACTATACTAACTTAGCATGCTGCATTACTTGCGGCTATCGAACCCAACTGAGGGGGCAGTACATGTTGGAGGAAAAACCTTGCAACGATTGCTGCGTTCATTTCTTCTGCAACTCCTGTGCCTTGTGCCAAGAATACCGTGAGCTCCAGAACCGTGGTTTCGATGTTGCCAGTGGGTGGAAAGGAAATGCGTCCCAGAATCGTGGAGTGGTGACAGCACCAGCTCTGCAGGGTGGCATGAATCGTCATTCTGATCACAAATAA

Protein Analysis

157

Amino Acids

17.17

Weight (kDa)

7.78

Isoelectric Point (pI)

37.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 19 - 117 3.7e-30 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 92
AccB7I CCANNNNNTGG 1 cut(s) 384
AciI CCGC 1 cut(s) 240
AcuI CTGAAG 1 cut(s) 64
AfaI GTAC 1 cut(s) 269
AfiI CCNNNNNNNGG 3 cut(s) 258, 259, 384
AflIII ACRYGT 1 cut(s) 270
AluBI AGCT 2 cut(s) 352, 431
AluI AGCT 2 cut(s) 352, 431
Alw21I GWGCWC 1 cut(s) 354
Alw26I GTCTC 1 cut(s) 157
AlwNI CAGNNNCTG 2 cut(s) 87, 96
AoxI GGCC 1 cut(s) 64
ApeKI GCWGC 4 cut(s) 99, 197, 228, 300
AseI ATTAAT 1 cut(s) 204
AspS9I GGNCC 1 cut(s) 57
AsuHPI GGTGA 1 cut(s) 430
AvaII GGWCC 1 cut(s) 57
BanII GRGCYC 1 cut(s) 354
Bbv12I GWGCWC 1 cut(s) 354
BbvI GCAGC 4 cut(s) 86, 209, 215, 287
BccI CCATC 1 cut(s) 48
BclI TGATCA 1 cut(s) 460
BcoDI GTCTC 1 cut(s) 157
BfmI CTRYAG 2 cut(s) 88, 434
BisI GCNGC 5 cut(s) 100, 198, 229, 241, 301
BlsI GCNGC 5 cut(s) 101, 199, 230, 242, 302
Bme18I GGWCC 1 cut(s) 57
BmgT120I GGNCC 1 cut(s) 57
BplI GAGNNNNNCTC 2 cut(s) 137, 169
BpmI CTGGAG 1 cut(s) 338
BsaJI CCNNGG 1 cut(s) 361
Bsc4I CCNNNNNNNGG 3 cut(s) 258, 259, 384
Bse1I ACTGG 3 cut(s) 67, 168, 378
BseDI CCNNGG 1 cut(s) 361
BseGI GGATG 2 cut(s) 59, 191
BseLI CCNNNNNNNGG 3 cut(s) 258, 259, 384
BseMII CTCAG 1 cut(s) 248
BseNI ACTGG 3 cut(s) 67, 168, 378
BseXI GCAGC 4 cut(s) 86, 209, 215, 287
BseYI CCCAGC 1 cut(s) 131
BshFI GGCC 1 cut(s) 66
BsiHKAI GWGCWC 1 cut(s) 354
BslFI GGGAC 1 cut(s) 385
BslI CCNNNNNNNGG 3 cut(s) 258, 259, 384
BsmAI GTCTC 1 cut(s) 157
BsmFI GGGAC 1 cut(s) 385
BsnI GGCC 1 cut(s) 66
Bsp1286I GDGCHC 1 cut(s) 354
Bsp143I GATC 1 cut(s) 460
BspACI CCGC 1 cut(s) 240
BspANI GGCC 1 cut(s) 66
BspCNI CTCAG 1 cut(s) 249
BspMAI CTGCAG 2 cut(s) 92, 438
BsrI ACTGG 3 cut(s) 67, 168, 378
BssECI CCNNGG 1 cut(s) 361
BssMI GATC 1 cut(s) 460
Bst4CI ACNGT 3 cut(s) 29, 347, 362
BstAPI GCANNNNNTGC 3 cut(s) 96, 237, 297
BstC8I GCNNGC 2 cut(s) 68, 226
BstDEI CTNAG 2 cut(s) 220, 257
BstDSI CCRYGG 1 cut(s) 361
BstF5I GGATG 2 cut(s) 59, 191
BstKTI GATC 1 cut(s) 463
BstMAI GTCTC 1 cut(s) 157
BstMBI GATC 1 cut(s) 460
BstMWI GCNNNNNNNGC 3 cut(s) 96, 237, 297
BstNSI RCATGY 2 cut(s) 228, 274
BstSFI CTRYAG 2 cut(s) 88, 434
BstV1I GCAGC 4 cut(s) 86, 209, 215, 287
BstXI CCANNNNNNTGG 3 cut(s) 31, 362, 410
BsuRI GGCC 1 cut(s) 66
BtgI CCRYGG 1 cut(s) 361
BtsCI GGATG 2 cut(s) 59, 191
BtsI GCAGTG 1 cut(s) 97
BtsIMutI CAGTG 4 cut(s) 60, 88, 97, 385
Cac8I GCNNGC 2 cut(s) 68, 226
CaiI CAGNNNCTG 2 cut(s) 87, 96
Cfr13I GGNCC 1 cut(s) 57
CseI GACGC 1 cut(s) 387
Csp6I GTAC 1 cut(s) 268
CviAII CATG 4 cut(s) 182, 225, 271, 445
CviJI RGCY 4 cut(s) 66, 243, 352, 431
CviKI_1 RGCY 4 cut(s) 66, 243, 352, 431
CviQI GTAC 1 cut(s) 268
DdeI CTNAG 2 cut(s) 220, 257
DpnI GATC 1 cut(s) 462
DpnII GATC 1 cut(s) 460
DrdI GACNNNNNNGTC 1 cut(s) 92
DseDI GACNNNNNNGTC 1 cut(s) 92
Ecl136II GAGCTC 1 cut(s) 352
Eco24I GRGCYC 1 cut(s) 354
Eco47I GGWCC 1 cut(s) 57
Eco53kI GAGCTC 1 cut(s) 352
Eco57I CTGAAG 1 cut(s) 64
EcoICRI GAGCTC 1 cut(s) 352
EcoT38I GRGCYC 1 cut(s) 354
FaeI CATG 4 cut(s) 185, 228, 274, 448
FaqI GGGAC 1 cut(s) 385
FatI CATG 4 cut(s) 181, 224, 270, 444
FbaI TGATCA 1 cut(s) 460
Fnu4HI GCNGC 5 cut(s) 100, 198, 229, 241, 301
FokI GGATG 2 cut(s) 66, 198
FriOI GRGCYC 1 cut(s) 354
Fsp4HI GCNGC 5 cut(s) 100, 198, 229, 241, 301
GluI GCNGC 5 cut(s) 100, 198, 229, 241, 301
GsaI CCCAGC 1 cut(s) 135
GsuI CTGGAG 1 cut(s) 338
HaeIII GGCC 1 cut(s) 66
HgaI GACGC 1 cut(s) 387
Hin1II CATG 4 cut(s) 185, 228, 274, 448
HinfI GANTC 2 cut(s) 406, 448
HphI GGTGA 1 cut(s) 430
Hpy188I TCNGA 1 cut(s) 460
Hpy188III TCNNGA 1 cut(s) 355
HpyCH4III ACNGT 3 cut(s) 29, 347, 362
HpyCH4V TGCA 7 cut(s) 90, 143, 197, 231, 291, 318, 436
HpyF10VI GCNNNNNNNGC 3 cut(s) 96, 237, 297
HpyF3I CTNAG 2 cut(s) 220, 257
Hsp92II CATG 4 cut(s) 185, 228, 274, 448
Ksp22I TGATCA 1 cut(s) 460
Kzo9I GATC 1 cut(s) 460
LmnI GCTCC 1 cut(s) 357
Lsp1109I GCAGC 4 cut(s) 86, 209, 215, 287
MaeIII GTNAC 2 cut(s) 83, 418
MalI GATC 1 cut(s) 462
MboI GATC 1 cut(s) 460
MboII GAAGA 1 cut(s) 304
MhlI GDGCHC 1 cut(s) 354
MluCI AATT 2 cut(s) 201, 205
MmeI TCCRAC 1 cut(s) 255
MnlI CCTC 2 cut(s) 252, 271
MseI TTAA 1 cut(s) 204
MwoI GCNNNNNNNGC 3 cut(s) 96, 237, 297
NdeII GATC 1 cut(s) 460
NlaIII CATG 4 cut(s) 185, 228, 274, 448
NmuCI GTSAC 2 cut(s) 83, 418
NspI RCATGY 2 cut(s) 228, 274
PaeI GCATGC 1 cut(s) 228
PciI ACATGT 1 cut(s) 270
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PfeI GAWTC 2 cut(s) 406, 448
PflMI CCANNNNNTGG 1 cut(s) 384
PkrI GCNGC 5 cut(s) 101, 199, 230, 242, 302
PscI ACATGT 1 cut(s) 270
PshBI ATTAAT 1 cut(s) 204
Psp124BI GAGCTC 1 cut(s) 354
PspFI CCCAGC 1 cut(s) 131
PspPI GGNCC 1 cut(s) 57
PstI CTGCAG 2 cut(s) 92, 438
PstNI CAGNNNCTG 2 cut(s) 87, 96
RsaI GTAC 1 cut(s) 269
RsaNI GTAC 1 cut(s) 268
SacI GAGCTC 1 cut(s) 354
SaqAI TTAA 1 cut(s) 204
SatI GCNGC 5 cut(s) 100, 198, 229, 241, 301
Sau3AI GATC 1 cut(s) 460
Sau96I GGNCC 1 cut(s) 57
SduI GDGCHC 1 cut(s) 354
SetI ASST 3 cut(s) 289, 354, 433
SfcI CTRYAG 2 cut(s) 88, 434
SinI GGWCC 1 cut(s) 57
SphI GCATGC 1 cut(s) 228
Sse9I AATT 2 cut(s) 201, 205
SsiI CCGC 1 cut(s) 240
SstI GAGCTC 1 cut(s) 354
TaaI ACNGT 3 cut(s) 29, 347, 362
TaqI TCGA 2 cut(s) 247, 369
TasI AATT 2 cut(s) 201, 205
TatI WGTACW 1 cut(s) 267
TauI GCSGC 1 cut(s) 243
TfiI GAWTC 2 cut(s) 406, 448
Tru1I TTAA 1 cut(s) 204
Tru9I TTAA 1 cut(s) 204
TscAI CASTG 4 cut(s) 67, 88, 97, 385
TseFI GTSAC 2 cut(s) 83, 418
TseI GCWGC 4 cut(s) 99, 197, 228, 300
Tsp45I GTSAC 2 cut(s) 83, 418
TspDTI ATGAA 2 cut(s) 296, 461
TspRI CASTG 4 cut(s) 67, 88, 97, 385
Van91I CCANNNNNTGG 1 cut(s) 384
VpaK11BI GGWCC 1 cut(s) 57
VspI ATTAAT 1 cut(s) 204
XceI RCATGY 2 cut(s) 228, 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.