FvH4_4g33931

PLAC8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0009650
Physical Location & Seq
Reverse (-)
31971879 .. 31972750
872 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g33931.t1

Sequence Viewer

Length: 591 bp
ATGTCCTCATCAAATCCGAAATCTCACGAGAAGCTGTTCGAGGGTGTTCGTAATTCGGCTACGGGAATTCATACGGTGTCCAGCAACCCCCATCCTGCTCAATATCATGACAATTGCATCTCTATGCTCGCTGCTCCGCCGTATCATCATTTCCCTCCTCCGATCCCCGAGCCCTGGAAATCCGGCATCTGTGGCTGCTTCTCCGATATGAACAGCTGCTGCTTAACATTCTTCTGTCCATGCATTACTTTTGGCCAGATTGCTGAGATCGTAGATAAAGGCTCTATATCTTGCGCGGTAAGTGGAGTAATTTACACGGCGATATATTGTTTGACTGGTTGTGCTTGCATCTATTCCTGCTTGTATCGTTCCAAACTGAGGCACCAGTATTATTTGGAGGAGAAACCTTGTTATGATTGTTTTGTTCATTTCTTCTGCGAGTCCTGCGCCTTGTGCCAGGAGTACCGTGAACTCCAAAGTCGCGGTTTCAACATGGCCATCGGATGGCAAGGAAATACGGATGGGAGGAACTATGATATGGCAATGGCTCCAGCCCCCCCAACGGTGGAGGGAGGCATGAGTCGGATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

197

Amino Acids

21.78

Weight (kDa)

6.21

Isoelectric Point (pI)

54.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 59 - 157 3.5e-33 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 381
AccB7I CCANNNNNTGG 1 cut(s) 504
AccII CGCG 2 cut(s) 296, 483
AciI CCGC 3 cut(s) 137, 296, 483
AclWI GGATC 1 cut(s) 157
AcoI YGGCCR 2 cut(s) 253, 495
AcsI RAATTY 1 cut(s) 66
AfaI GTAC 1 cut(s) 464
AfiI CCNNNNNNNGG 5 cut(s) 174, 378, 504, 562, 565
AgsI TTSAA 1 cut(s) 490
AjnI CCWGG 2 cut(s) 173, 456
AluBI AGCT 2 cut(s) 34, 216
AluI AGCT 2 cut(s) 34, 216
AlwI GGATC 1 cut(s) 157
AlwNI CAGNNNCTG 1 cut(s) 219
Ama87I CYCGRG 1 cut(s) 167
AoxI GGCC 2 cut(s) 253, 495
ApeKI GCWGC 4 cut(s) 131, 195, 216, 219
ApoI RAATTY 1 cut(s) 66
Asp700I GAANNNNTTC 1 cut(s) 35
AspLEI GCGC 2 cut(s) 296, 449
AvaI CYCGRG 1 cut(s) 167
BalI TGGCCA 2 cut(s) 255, 497
BanI GGYRCC 1 cut(s) 381
BanII GRGCYC 1 cut(s) 174
BauI CACGAG 1 cut(s) 26
BbvI GCAGC 4 cut(s) 118, 182, 203, 206
BccI CCATC 4 cut(s) 99, 498, 506, 515
BceAI ACGGC 2 cut(s) 124, 333
BciT130I CCWGG 2 cut(s) 175, 458
BisI GCNGC 4 cut(s) 132, 196, 217, 220
BlsI GCNGC 4 cut(s) 133, 197, 218, 221
Bme1390I CCNGG 2 cut(s) 175, 458
BmeT110I CYCGRG 1 cut(s) 167
BmiI GGNNCC 2 cut(s) 383, 549
BmrFI CCNGG 2 cut(s) 175, 458
BmsI GCATC 3 cut(s) 126, 195, 357
BpmI CTGGAG 1 cut(s) 534
BsaJI CCNNGG 1 cut(s) 173
Bsc4I CCNNNNNNNGG 5 cut(s) 174, 378, 504, 562, 565
Bse1I ACTGG 2 cut(s) 340, 385
Bse3DI GCAATG 1 cut(s) 549
BseBI CCWGG 2 cut(s) 175, 458
BseDI CCNNGG 1 cut(s) 173
BseGI GGATG 3 cut(s) 91, 509, 526
BseLI CCNNNNNNNGG 5 cut(s) 174, 378, 504, 562, 565
BseMI GCAATG 1 cut(s) 549
BseMII CTCAG 2 cut(s) 255, 368
BseNI ACTGG 2 cut(s) 340, 385
BseRI GAGGAG 2 cut(s) 147, 413
BseXI GCAGC 4 cut(s) 118, 182, 203, 206
Bsh1236I CGCG 2 cut(s) 296, 483
BshFI GGCC 2 cut(s) 255, 497
BshNI GGYRCC 1 cut(s) 381
BsiHKCI CYCGRG 1 cut(s) 167
BsiSI CCGG 1 cut(s) 183
BslI CCNNNNNNNGG 5 cut(s) 174, 378, 504, 562, 565
BsnI GGCC 2 cut(s) 255, 497
BsoBI CYCGRG 1 cut(s) 167
Bsp1286I GDGCHC 1 cut(s) 174
Bsp143I GATC 2 cut(s) 162, 267
BspACI CCGC 3 cut(s) 137, 296, 483
BspANI GGCC 2 cut(s) 255, 497
BspCNI CTCAG 2 cut(s) 256, 369
BspFNI CGCG 2 cut(s) 296, 483
BspHI TCATGA 1 cut(s) 106
BspLI GGNNCC 2 cut(s) 383, 549
BspPI GGATC 1 cut(s) 157
BspT107I GGYRCC 1 cut(s) 381
BsrDI GCAATG 1 cut(s) 549
BsrI ACTGG 2 cut(s) 340, 385
BssECI CCNNGG 1 cut(s) 173
BssMI GATC 2 cut(s) 162, 267
BssSI CACGAG 1 cut(s) 26
Bst2BI CACGAG 1 cut(s) 26
Bst2UI CCWGG 2 cut(s) 175, 458
Bst4CI ACNGT 3 cut(s) 76, 467, 565
BstC8I GCNNGC 2 cut(s) 129, 346
BstDEI CTNAG 2 cut(s) 264, 377
BstF5I GGATG 3 cut(s) 91, 509, 526
BstFNI CGCG 2 cut(s) 296, 483
BstHHI GCGC 2 cut(s) 296, 449
BstKTI GATC 2 cut(s) 165, 270
BstMBI GATC 2 cut(s) 162, 267
BstMWI GCNNNNNNNGC 3 cut(s) 192, 444, 453
BstNI CCWGG 2 cut(s) 175, 458
BstSCI CCNGG 2 cut(s) 173, 456
BstUI CGCG 2 cut(s) 296, 483
BstV1I GCAGC 4 cut(s) 118, 182, 203, 206
BsuRI GGCC 2 cut(s) 255, 497
BtsCI GGATG 3 cut(s) 91, 509, 526
Cac8I GCNNGC 2 cut(s) 129, 346
CaiI CAGNNNCTG 1 cut(s) 219
CciI TCATGA 1 cut(s) 106
CfoI GCGC 2 cut(s) 296, 449
Csp6I GTAC 1 cut(s) 463
CviAII CATG 4 cut(s) 107, 240, 493, 577
CviQI GTAC 1 cut(s) 463
DdeI CTNAG 2 cut(s) 264, 377
DpnI GATC 2 cut(s) 164, 269
DpnII GATC 2 cut(s) 162, 267
EaeI YGGCCR 2 cut(s) 253, 495
EciI GGCGGA 1 cut(s) 126
Eco24I GRGCYC 1 cut(s) 174
Eco88I CYCGRG 1 cut(s) 167
EcoRI GAATTC 1 cut(s) 66
EcoRII CCWGG 2 cut(s) 173, 456
EcoT22I ATGCAT 1 cut(s) 245
EcoT38I GRGCYC 1 cut(s) 174
FaeI CATG 4 cut(s) 110, 243, 496, 580
FatI CATG 4 cut(s) 106, 239, 492, 576
Fnu4HI GCNGC 4 cut(s) 132, 196, 217, 220
FokI GGATG 3 cut(s) 78, 516, 533
FriOI GRGCYC 1 cut(s) 174
Fsp4HI GCNGC 4 cut(s) 132, 196, 217, 220
GlaI GCGC 2 cut(s) 295, 448
GluI GCNGC 4 cut(s) 132, 196, 217, 220
GsuI CTGGAG 1 cut(s) 534
HaeIII GGCC 2 cut(s) 255, 497
HapII CCGG 1 cut(s) 183
HhaI GCGC 2 cut(s) 296, 449
Hin1II CATG 4 cut(s) 110, 243, 496, 580
Hin6I GCGC 2 cut(s) 294, 447
HinP1I GCGC 2 cut(s) 294, 447
HinfI GANTC 2 cut(s) 440, 580
HpaII CCGG 1 cut(s) 183
Hpy166II GTNNAC 1 cut(s) 470
Hpy188I TCNGA 5 cut(s) 18, 162, 205, 503, 585
Hpy188III TCNNGA 2 cut(s) 26, 107
Hpy8I GTNNAC 1 cut(s) 470
HpyCH4III ACNGT 3 cut(s) 76, 467, 565
HpyCH4V TGCA 3 cut(s) 117, 243, 348
HpyF10VI GCNNNNNNNGC 3 cut(s) 192, 444, 453
HpyF3I CTNAG 2 cut(s) 264, 377
Hsp92II CATG 4 cut(s) 110, 243, 496, 580
HspAI GCGC 2 cut(s) 294, 447
Kzo9I GATC 2 cut(s) 162, 267
LmnI GCTCC 2 cut(s) 139, 553
Lsp1109I GCAGC 4 cut(s) 118, 182, 203, 206
LweI GCATC 3 cut(s) 126, 195, 357
MalI GATC 2 cut(s) 164, 269
MboI GATC 2 cut(s) 162, 267
MboII GAAGA 2 cut(s) 223, 424
MfeI CAATTG 1 cut(s) 112
MhlI GDGCHC 1 cut(s) 174
MlsI TGGCCA 2 cut(s) 255, 497
MluCI AATT 4 cut(s) 52, 66, 112, 309
MluNI TGGCCA 2 cut(s) 255, 497
MlyI GAGTC 2 cut(s) 449, 589
MmeI TCCRAC 1 cut(s) 563
MnlI CCTC 9 cut(s) 16, 34, 165, 168, 372, 391, 519, 562, 566
Mox20I TGGCCA 2 cut(s) 255, 497
Mph1103I ATGCAT 1 cut(s) 245
MroXI GAANNNNTTC 1 cut(s) 35
MscI TGGCCA 2 cut(s) 255, 497
MseI TTAA 1 cut(s) 224
MslI CAYNNNNRTG 1 cut(s) 122
Msp20I TGGCCA 2 cut(s) 255, 497
MspA1I CMGCKG 1 cut(s) 216
MspI CCGG 1 cut(s) 183
MspR9I CCNGG 2 cut(s) 175, 458
MunI CAATTG 1 cut(s) 112
MvaI CCWGG 2 cut(s) 175, 458
MvnI CGCG 2 cut(s) 296, 483
MwoI GCNNNNNNNGC 3 cut(s) 192, 444, 453
NdeII GATC 2 cut(s) 162, 267
NlaIII CATG 4 cut(s) 110, 243, 496, 580
NlaIV GGNNCC 2 cut(s) 383, 549
NsiI ATGCAT 1 cut(s) 245
PagI TCATGA 1 cut(s) 106
PdmI GAANNNNTTC 1 cut(s) 35
PflMI CCANNNNNTGG 1 cut(s) 504
PkrI GCNGC 4 cut(s) 133, 197, 218, 221
PleI GAGTC 2 cut(s) 448, 588
PpsI GAGTC 2 cut(s) 448, 588
Psp6I CCWGG 2 cut(s) 173, 456
PspGI CCWGG 2 cut(s) 173, 456
PspN4I GGNNCC 2 cut(s) 383, 549
PstNI CAGNNNCTG 1 cut(s) 219
PvuII CAGCTG 1 cut(s) 216
RsaI GTAC 1 cut(s) 464
RsaNI GTAC 1 cut(s) 463
RseI CAYNNNNRTG 1 cut(s) 122
SaqAI TTAA 1 cut(s) 224
SatI GCNGC 4 cut(s) 132, 196, 217, 220
Sau3AI GATC 2 cut(s) 162, 267
SchI GAGTC 2 cut(s) 449, 589
ScrFI CCNGG 2 cut(s) 175, 458
SduI GDGCHC 1 cut(s) 174
SetI ASST 3 cut(s) 36, 218, 409
SfaNI GCATC 3 cut(s) 126, 195, 357
SmiMI CAYNNNNRTG 1 cut(s) 122
Sse9I AATT 4 cut(s) 52, 66, 112, 309
SsiI CCGC 3 cut(s) 137, 296, 483
StyD4I CCNGG 2 cut(s) 173, 456
TaaI ACNGT 3 cut(s) 76, 467, 565
TaqI TCGA 1 cut(s) 39
TasI AATT 4 cut(s) 52, 66, 112, 309
Tru1I TTAA 1 cut(s) 224
Tru9I TTAA 1 cut(s) 224
TseI GCWGC 4 cut(s) 131, 195, 216, 219
TspDTI ATGAA 3 cut(s) 59, 224, 416
TspGWI ACGGA 1 cut(s) 533
Van91I CCANNNNNTGG 1 cut(s) 504
XapI RAATTY 1 cut(s) 66
XmnI GAANNNNTTC 1 cut(s) 35
Zsp2I ATGCAT 1 cut(s) 245
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.