FvH4_2g06550

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0011520
Physical Location & Seq
Forward (+)
5435624 .. 5439310
3687 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g06550.t1

Sequence Viewer

Length: 2814 bp
ATGGAGTTGGCTGCAGCCGTGGCCGGCATTTTGAGTTGTTTATGTCCCAAGAATGTTATACAAGAAACGACAGGGCAGCAGTGCAAGTATCTTGATTACAATGCAAAAGCAAAGATAGAAGAATTGAGGTCGAAAGTGGAGGAATTAAGAGCACTTGTGGCGGATGTAAATAAAAAGCTGGAGGAAACATGTCTCCAAGAAGGAAAGGAGCCAACAGAACAGGTCAAGTTGTGGCTGAGAAAGGTTCAGAGCTTTGATAATGACAAGCTGAATAAACTACTATCTTGGGAAGAGAAGAGATGTTTATGTGGGTGTCTTCCAGATTATTACTCTCGAATGAAGTTCGGGAAACTAAACGAAAGGAAGAATCAGGAGGTGGATGAGCTTCTGAGGAGAGGACAGTTTCAAGATGATGCATTGGCTCACTTATTGTTAGAGAATATAAAGATGCTTCCTGTGACAGCAGCAGTGGTTAATGAAACAACTAAAAGAAAGTTGGAAGAGATATGGGAGCATTTAATGGCAGATTATGAAGTGCAAAGAATTGGTGTTTATGGAATGGGCGGGGTTGGAAAAACAACAATTCTGAAAGAAATAAATAACAGACTCAAAAAAGTGATGACAGATGGGATGATCACTTGCTTTGACAATGTTGTTTGGGTCACAATACCAAAGGAGTCCAACCCCGAGAAATTGCAAATGGCGATTGCCAAAGCCGTGAGGTTGAATCTCCCGGAAGATTGGAATATGATGGACAAGGCAGCAGAGTTGCTGAAAGCATTACAACGAAGGAAGAGGCTTTTGCTAATTATTGATGATGTATGGACACCATTTTCCTTGGAAGATATTGGAATTCCAGCACCTACCAGAGCCAATGGCTGCAAGCTAATAATAACTACTAGATCATTGAGGGTGTGTCGAGGCATGGAGACAAACAAAGAAGTTGAGGTAAAAGTTCTTTCTGAGGAAGAATCATGGACCCTTTTCACTGATAAAGTTGGTTCTGTTGCGTTACAAAATCCAGTAATTACAGTTTTAGCAAGGGAAATAGCGAAAGAATTTGGTGGTCTTCCACTAGCCATTATCACAGTTGGAAGAGCCTTGAGAAAAGATGACACTACTCGATCATGTTTTCTGTATTGTGCAATGTATCCTGAAGATCATCAAATTGAAGCCAGAGAACTGATAGAATATTGGATATGGGAGGGTTTGCTTGGTGATGGGGGAAGGATACAGGCCAATATATGGAAGGGTGAAATGATTTTGGCTGATCTTAAAGATTCTTGCCTGCTGGACAATGTTGATGCAGATGGTGGTGTTGAATGTGTCAAAATGCATGACCTAATAAGGGATATGGCAATCGGTATTACAAATACAAGTCCTCGATTCGTCATTAGTGCTGGGGTTGGAGTAGAAAAGGCATCACTTGGTGAGGAGGGGATCGAAGATGTTGAGAGGATTTCAATTATGTATAATGAAATGAAATACCTTCCTGGCAAACCATTGTGTTCCAAGCTCTCATGTTTGCTGCTGCAGTATAGCTGTATTGCCAATGGTATCTCACATCATTTCTTCTGCCACATGCCCAAGCTCAGAGTACTTGATCTCTCTTTCACTGGAATTCTATGTATACCTGACTCCATTACTGAGCTAAGCAATTTGCGTGCACTGCTATTGCGTAGCTGTTGGAACCTAAGTAGTCTTCCTTCTCTAGCAACACTCGAGTCATTGTGGGTTTTGGATCTTTCGTACACTCGCATCACAGAATTGCCACCAGGTATGAAAGAGTTACGCCAGCTTAAGCGCCTCAATCTTTCTTATACTAAATTGGACAGTTTTCCGGAAGGACTGTTAACCACTTTAAATCTGCTAGAAGAATTGCTCCTTTATCGTTGTAAGTGTGAATTTGGTTCAAAGTTTGTTGATGAACTCATCACTGCAAGAAATATTGTTGTTCTTGAAGTTGAATTCTCAACCTCCAGAGTGTTTGATGTCTATACAAGATCAGGGCATTGGAACCTTCTGCGGAGTTTTAGATCCGGCATATGCTCATATGGGGATGACTTGGGAAAGAATAGTATAGACTACAATGGCAATTTTTTTCTCAGTGGTAATCCGGTGTTGCTTCCTGATCACACTTATGAATTGCGACTCCTCCACTGCTATGATGTTGATCGATTGTCAAGGTGCTTGTCAGATTCAATTAAGTTGAAAAAATGTGTCATAACACACTGCGATCAAATGGAGAGTATACTGATGCCAAGGGAGAACAACTTAAGCTGTGTAAATACTTTAGAGCTTGGTTTCCTACAAAACTTTAAGAGTCTCTGCACGGGTGCCATTCCCTCAGGAGCTTTAGGTGCCCTGAAGATACTGCAAATCAGTTACTGTAACTCTTTAGAGAGTGTACTCAACCCAAGGCTGATTGAACATCTCGGAAACCTTGAAGAACTTTCTGTTGAGCATTGTACAAAATTGGAGGTGGTGATAGAGGAGGAGCATTCGATCCACAATGCTGAAGTCAGCCTTTCAAGATTGAAGAGCTTCAAGTTGTGTGATTTGAGAGAACTGAAGAGCATTTATAGTGAAGAAGTAATTTGCTTATCTCTATGCACCATCAATATTGAGGGATGCAATAAATTGAGGAGACTTCCTTTTAGGATTTCAATAGATGAAGGATACCAGCAAAGACAACCATCAATCACTCCCCCCATAAGAAAAATTAAAGCAGAGCAAGTGTGGTGGGATTCAGTGGAGTTTGCTAATCCTAGTGTAAAGACCTTCCTTCAACACAGGCTGGAGTTGCCTAGCTAG

Protein Analysis

938

Amino Acids

106.43

Weight (kDa)

5.85

Isoelectric Point (pI)

48.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NB-ARC PF00931 164 - 335 1.1e-41 NB-ARC domain
TniB PF05621 165 - 274 1.2e-06 Bacterial TniB protein
AAA_22 PF13401 184 - 274 2.8e-06 AAA domain
WHD_DRP PF23559 383 - 453 2.1e-10 Disease resistance protein Winged helix domain
LRR_14 PF23598 529 - 659 8e-12 Leucine-rich repeat region
LRR_8 PF13855 577 - 630 1.5e-06 Leucine rich repeat
LRR_RPS2 PF23247 772 - 894 9.1e-16 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 2336, 2360
AccB7I CCANNNNNTGG 1 cut(s) 1245
AccI GTMKAC 2 cut(s) 1630, 2251
AccIII TCCGGA 1 cut(s) 1840
AciI CCGC 3 cut(s) 161, 564, 2026
AclWI GGATC 4 cut(s) 1448, 1749, 2031, 2500
AcoI YGGCCR 1 cut(s) 21
AcsI RAATTY 5 cut(s) 852, 1058, 1620, 1904, 1967
AcuI CTGAAG 4 cut(s) 1176, 2387, 2538, 2591
AdeI CACNNNGTG 1 cut(s) 1430
AfaI GTAC 4 cut(s) 1599, 1751, 2409, 2470
AfiI CCNNNNNNNGG 2 cut(s) 1245, 1348
AflII CTTAAG 2 cut(s) 1799, 2275
AflIII ACRYGT 1 cut(s) 188
AjnI CCWGG 2 cut(s) 1492, 1774
Alw21I GWGCWC 2 cut(s) 154, 1669
Alw26I GTCTC 4 cut(s) 197, 923, 2330, 2641
Alw44I GTGCAC 1 cut(s) 1665
AlwI GGATC 4 cut(s) 1448, 1749, 2031, 2500
AlwNI CAGNNNCTG 1 cut(s) 2388
Ama87I CYCGRG 2 cut(s) 686, 1721
Aor13HI TCCGGA 1 cut(s) 1840
AoxI GGCC 2 cut(s) 21, 1236
ApaLI GTGCAC 1 cut(s) 1665
ApeKI GCWGC 8 cut(s) 11, 14, 76, 464, 761, 879, 1528, 1531
ApoI RAATTY 5 cut(s) 852, 1058, 1620, 1904, 1967
Asp700I GAANNNNTTC 1 cut(s) 2543
AspLEI GCGC 1 cut(s) 1806
AspS9I GGNCC 1 cut(s) 978
AsuC2I CCSGG 1 cut(s) 734
AsuHPI GGTGA 4 cut(s) 1229, 1265, 1442, 2497
AvaI CYCGRG 2 cut(s) 686, 1721
AvaII GGWCC 1 cut(s) 978
AxyI CCTNAGG 1 cut(s) 2347
BaeGI GKGCMC 2 cut(s) 1669, 2365
BanI GGYRCC 2 cut(s) 2336, 2360
BarI GAAGNNNNNNTAC 2 cut(s) 1690, 1722
BbsI GAAGAC 3 cut(s) 308, 1061, 1694
Bbv12I GWGCWC 2 cut(s) 154, 1669
BbvI GCAGC 7 cut(s) 26, 88, 476, 773, 866, 1515, 1518
BccI CCATC 6 cut(s) 620, 745, 1214, 1304, 2624, 2704
BceAI ACGGC 2 cut(s) 2, 701
BciT130I CCWGG 2 cut(s) 1494, 1776
BciVI GTATCC 3 cut(s) 1161, 1224, 2672
BclI TGATCA 2 cut(s) 633, 2131
BcnI CCSGG 1 cut(s) 734
BcoDI GTCTC 4 cut(s) 197, 923, 2330, 2641
BfaI CTAG 7 cut(s) 900, 1076, 1712, 1871, 2769, 2808, 2812
BfmI CTRYAG 2 cut(s) 12, 1532
BfoI RGCGCY 1 cut(s) 1807
BfrI CTTAAG 2 cut(s) 1799, 2275
BfuI GTATCC 3 cut(s) 1161, 1224, 2672
BisI GCNGC 8 cut(s) 12, 15, 77, 465, 762, 880, 1529, 1532
BlpI GCTNAGC 1 cut(s) 1652
BlsI GCNGC 8 cut(s) 13, 16, 78, 466, 763, 881, 1530, 1533
BmcAI AGTACT 1 cut(s) 1599
Bme1390I CCNGG 3 cut(s) 734, 1494, 1776
Bme18I GGWCC 1 cut(s) 978
BmeT110I CYCGRG 2 cut(s) 686, 1721
BmgT120I GGNCC 1 cut(s) 978
BmiI GGNNCC 6 cut(s) 210, 980, 1691, 2018, 2338, 2362
BmrFI CCNGG 3 cut(s) 734, 1494, 1776
BmsI GCATC 7 cut(s) 403, 438, 1294, 1430, 1767, 2247, 2621
BpiI GAAGAC 3 cut(s) 308, 1061, 1694
BpmI CTGGAG 2 cut(s) 200, 1963
Bpu1102I GCTNAGC 1 cut(s) 1652
BpuEI CTTGAG 1 cut(s) 1123
BpuMI CCSGG 1 cut(s) 734
Bsa29I ATCGAT 1 cut(s) 2176
BsaBI GATNNNNATC 1 cut(s) 2172
BsaJI CCNNGG 4 cut(s) 18, 837, 2261, 2417
BsaWI WCCGGW 2 cut(s) 1840, 2116
Bsc4I CCNNNNNNNGG 2 cut(s) 1245, 1348
Bse118I RCCGGY 1 cut(s) 23
Bse1I ACTGG 2 cut(s) 1022, 1621
Bse21I CCTNAGG 1 cut(s) 2347
Bse3DI GCAATG 1 cut(s) 1152
Bse8I GATNNNNATC 1 cut(s) 2172
BseAI TCCGGA 1 cut(s) 1840
BseBI CCWGG 2 cut(s) 1494, 1776
BseCI ATCGAT 1 cut(s) 2176
BseDI CCNNGG 4 cut(s) 18, 837, 2261, 2417
BseGI GGATG 5 cut(s) 169, 385, 636, 2065, 2636
BseJI GATNNNNATC 1 cut(s) 2172
BseLI CCNNNNNNNGG 2 cut(s) 1245, 1348
BseMI GCAATG 1 cut(s) 1152
BseMII CTCAG 7 cut(s) 227, 380, 954, 1606, 1638, 2119, 2361
BseNI ACTGG 2 cut(s) 1022, 1621
BseRI GAGGAG 6 cut(s) 406, 1448, 2144, 2507, 2510, 2659
BseSI GKGCMC 2 cut(s) 1669, 2365
BseXI GCAGC 7 cut(s) 26, 88, 476, 773, 866, 1515, 1518
BseYI CCCAGC 1 cut(s) 1400
BsgI GTGCAG 1 cut(s) 2314
BshFI GGCC 2 cut(s) 23, 1238
BshNI GGYRCC 2 cut(s) 2336, 2360
BshVI ATCGAT 1 cut(s) 2176
BsiHKAI GWGCWC 2 cut(s) 154, 1669
BsiHKCI CYCGRG 2 cut(s) 686, 1721
BsiSI CCGG 5 cut(s) 24, 734, 1841, 2040, 2117
BslFI GGGAC 1 cut(s) 30
BslI CCNNNNNNNGG 2 cut(s) 1245, 1348
BsmAI GTCTC 4 cut(s) 197, 923, 2330, 2641
BsmFI GGGAC 1 cut(s) 30
BsmI GAATGC 1 cut(s) 2500
BsnI GGCC 2 cut(s) 23, 1238
BsoBI CYCGRG 2 cut(s) 686, 1721
Bsp1286I GDGCHC 3 cut(s) 154, 1669, 2365
Bsp13I TCCGGA 1 cut(s) 1840
Bsp1407I TGTACA 1 cut(s) 2468
Bsp1720I GCTNAGC 1 cut(s) 1652
BspACI CCGC 3 cut(s) 161, 564, 2026
BspANI GGCC 2 cut(s) 23, 1238
BspCNI CTCAG 7 cut(s) 228, 381, 955, 1605, 1639, 2118, 2360
BspDI ATCGAT 1 cut(s) 2176
BspEI TCCGGA 1 cut(s) 1840
BspLI GGNNCC 6 cut(s) 210, 980, 1691, 2018, 2338, 2362
BspMAI CTGCAG 2 cut(s) 16, 1536
BspPI GGATC 4 cut(s) 1448, 1749, 2031, 2500
BspQI GCTCTTC 3 cut(s) 1090, 2534, 2567
BspT107I GGYRCC 2 cut(s) 2336, 2360
BspTI CTTAAG 2 cut(s) 1799, 2275
BsrDI GCAATG 1 cut(s) 1152
BsrFI RCCGGY 1 cut(s) 23
BsrGI TGTACA 1 cut(s) 2468
BsrI ACTGG 2 cut(s) 1022, 1621
BssAI RCCGGY 1 cut(s) 23
BssECI CCNNGG 4 cut(s) 18, 837, 2261, 2417
BssNAI GTATAC 2 cut(s) 1631, 2252
BssT1I CCWWGG 3 cut(s) 837, 2261, 2417
Bst1107I GTATAC 2 cut(s) 1631, 2252
Bst2UI CCWGG 2 cut(s) 1494, 1776
Bst4CI ACNGT 6 cut(s) 402, 1033, 1090, 1835, 1851, 2390
Bst6I CTCTTC 7 cut(s) 285, 290, 495, 788, 1090, 2534, 2567
BstAFI CTTAAG 2 cut(s) 1799, 2275
BstAUI TGTACA 1 cut(s) 2468
BstC8I GCNNGC 5 cut(s) 25, 884, 1289, 1665, 1796
BstDEI CTNAG 9 cut(s) 236, 389, 963, 1592, 1647, 1652, 1694, 2105, 2347
BstDSI CCRYGG 1 cut(s) 18
BstF5I GGATG 5 cut(s) 169, 385, 636, 2065, 2636
BstH2I RGCGCY 1 cut(s) 1807
BstHHI GCGC 1 cut(s) 1806
BstMAI GTCTC 4 cut(s) 197, 923, 2330, 2641
BstMWI GCNNNNNNNGC 5 cut(s) 20, 158, 1669, 2360, 2803
BstNI CCWGG 2 cut(s) 1494, 1776
BstNSI RCATGY 2 cut(s) 192, 1585
BstSCI CCNGG 3 cut(s) 732, 1492, 1774
BstSFI CTRYAG 2 cut(s) 12, 1532
BstSLI GKGCMC 2 cut(s) 1669, 2365
BstV1I GCAGC 7 cut(s) 26, 88, 476, 773, 866, 1515, 1518
BstV2I GAAGAC 3 cut(s) 308, 1061, 1694
BstX2I RGATCY 2 cut(s) 1741, 2036
BstYI RGATCY 2 cut(s) 1741, 2036
BstZ17I GTATAC 2 cut(s) 1631, 2252
Bsu15I ATCGAT 1 cut(s) 2176
Bsu36I CCTNAGG 1 cut(s) 2347
BsuI GTATCC 3 cut(s) 1161, 1224, 2672
BsuRI GGCC 2 cut(s) 23, 1238
BsuTUI ATCGAT 1 cut(s) 2176
BtgI CCRYGG 1 cut(s) 18
BtsCI GGATG 5 cut(s) 169, 385, 636, 2065, 2636
BtsI GCAGTG 6 cut(s) 86, 474, 1667, 1935, 2158, 2230
Cac8I GCNNGC 5 cut(s) 25, 884, 1289, 1665, 1796
CaiI CAGNNNCTG 1 cut(s) 2388
CfoI GCGC 1 cut(s) 1806
Cfr10I RCCGGY 1 cut(s) 23
Cfr13I GGNCC 1 cut(s) 978
ClaI ATCGAT 1 cut(s) 2176
CsiI ACCWGGT 1 cut(s) 1774
Csp6I GTAC 4 cut(s) 1598, 1750, 2408, 2469
CspCI CAANNNNNGTGG 2 cut(s) 2723, 2758
CviAII CATG 7 cut(s) 189, 925, 975, 1128, 1337, 1521, 1582
CviQI GTAC 4 cut(s) 1598, 1750, 2408, 2469
DdeI CTNAG 9 cut(s) 236, 389, 963, 1592, 1647, 1652, 1694, 2105, 2347
DraI TTTAAA 1 cut(s) 1863
DraIII CACNNNGTG 1 cut(s) 1430
EaeI YGGCCR 1 cut(s) 21
Eam1104I CTCTTC 7 cut(s) 285, 290, 495, 788, 1090, 2534, 2567
EarI CTCTTC 7 cut(s) 285, 290, 495, 788, 1090, 2534, 2567
EciI GGCGGA 1 cut(s) 176
Eco130I CCWWGG 3 cut(s) 837, 2261, 2417
Eco47I GGWCC 1 cut(s) 978
Eco57I CTGAAG 4 cut(s) 1176, 2387, 2538, 2591
Eco81I CCTNAGG 1 cut(s) 2347
Eco88I CYCGRG 2 cut(s) 686, 1721
EcoRI GAATTC 3 cut(s) 852, 1620, 1967
EcoRII CCWGG 2 cut(s) 1492, 1774
EcoT14I CCWWGG 3 cut(s) 837, 2261, 2417
EcoT22I ATGCAT 2 cut(s) 418, 1338
ErhI CCWWGG 3 cut(s) 837, 2261, 2417
FaeI CATG 7 cut(s) 192, 928, 978, 1131, 1340, 1524, 1585
FalI AAGNNNNNCTT 2 cut(s) 2511, 2543
FaqI GGGAC 1 cut(s) 30
FatI CATG 7 cut(s) 188, 924, 974, 1127, 1336, 1520, 1581
FauI CCCGC 1 cut(s) 557
FauNDI CATATG 2 cut(s) 2045, 2053
FbaI TGATCA 2 cut(s) 633, 2131
FblI GTMKAC 2 cut(s) 1630, 2251
Fnu4HI GCNGC 8 cut(s) 12, 15, 77, 465, 762, 880, 1529, 1532
FokI GGATG 5 cut(s) 176, 392, 643, 2072, 2643
Fsp4HI GCNGC 8 cut(s) 12, 15, 77, 465, 762, 880, 1529, 1532
FspBI CTAG 7 cut(s) 900, 1076, 1712, 1871, 2769, 2808, 2812
GlaI GCGC 1 cut(s) 1805
GluI GCNGC 8 cut(s) 12, 15, 77, 465, 762, 880, 1529, 1532
GsaI CCCAGC 1 cut(s) 1404
GsuI CTGGAG 2 cut(s) 200, 1963
HaeII RGCGCY 1 cut(s) 1807
HaeIII GGCC 2 cut(s) 23, 1238
HapII CCGG 5 cut(s) 24, 734, 1841, 2040, 2117
HhaI GCGC 1 cut(s) 1806
Hin1II CATG 7 cut(s) 192, 928, 978, 1131, 1340, 1524, 1585
Hin6I GCGC 1 cut(s) 1804
HinP1I GCGC 1 cut(s) 1804
HincII GTYRAC 1 cut(s) 1854
HindII GTYRAC 1 cut(s) 1854
HpaI GTTAAC 1 cut(s) 1854
HpaII CCGG 5 cut(s) 24, 734, 1841, 2040, 2117
HphI GGTGA 4 cut(s) 1229, 1265, 1442, 2497
Hpy166II GTNNAC 6 cut(s) 1631, 1667, 1752, 1854, 2252, 2408
Hpy188I TCNGA 7 cut(s) 249, 390, 588, 964, 1595, 2197, 2438
Hpy8I GTNNAC 6 cut(s) 1631, 1667, 1752, 1854, 2252, 2408
HpyCH4III ACNGT 6 cut(s) 402, 1033, 1090, 1835, 1851, 2390
HpyF10VI GCNNNNNNNGC 5 cut(s) 20, 158, 1669, 2360, 2803
HpyF3I CTNAG 9 cut(s) 236, 389, 963, 1592, 1647, 1652, 1694, 2105, 2347
Hsp92II CATG 7 cut(s) 192, 928, 978, 1131, 1340, 1524, 1585
HspAI GCGC 1 cut(s) 1804
Kpn2I TCCGGA 1 cut(s) 1840
KroI GCCGGC 1 cut(s) 23
KroNI GCCGGC 1 cut(s) 25
Ksp22I TGATCA 2 cut(s) 633, 2131
KspAI GTTAAC 1 cut(s) 1854
LguI GCTCTTC 3 cut(s) 1090, 2534, 2567
LmnI GCTCC 5 cut(s) 208, 511, 1887, 2351, 2497
Lsp1109I GCAGC 7 cut(s) 26, 88, 476, 773, 866, 1515, 1518
LweI GCATC 7 cut(s) 403, 438, 1294, 1430, 1767, 2247, 2621
MabI ACCWGGT 1 cut(s) 1774
MaeI CTAG 7 cut(s) 900, 1076, 1712, 1871, 2769, 2808, 2812
MaeIII GTNAC 6 cut(s) 457, 661, 1011, 1788, 2384, 2390
MflI RGATCY 2 cut(s) 1741, 2036
MhlI GDGCHC 3 cut(s) 154, 1669, 2365
MlyI GAGTC 6 cut(s) 600, 686, 1631, 1733, 2145, 2332
MmeI TCCRAC 6 cut(s) 477, 550, 705, 1072, 1387, 1667
Mph1103I ATGCAT 2 cut(s) 418, 1338
MroI TCCGGA 1 cut(s) 1840
MroNI GCCGGC 1 cut(s) 23
MroXI GAANNNNTTC 1 cut(s) 2543
MslI CAYNNNNRTG 2 cut(s) 2139, 2163
MspCI CTTAAG 2 cut(s) 1799, 2275
MspI CCGG 5 cut(s) 24, 734, 1841, 2040, 2117
MspR9I CCNGG 3 cut(s) 734, 1494, 1776
Mva1269I GAATGC 1 cut(s) 2500
MvaI CCWGG 2 cut(s) 1494, 1776
MwoI GCNNNNNNNGC 5 cut(s) 20, 158, 1669, 2360, 2803
NaeI GCCGGC 1 cut(s) 25
NciI CCSGG 1 cut(s) 734
NdeI CATATG 2 cut(s) 2045, 2053
NgoMIV GCCGGC 1 cut(s) 23
NlaIII CATG 7 cut(s) 192, 928, 978, 1131, 1340, 1524, 1585
NlaIV GGNNCC 6 cut(s) 210, 980, 1691, 2018, 2338, 2362
NmuCI GTSAC 2 cut(s) 457, 661
NsiI ATGCAT 2 cut(s) 418, 1338
NspI RCATGY 2 cut(s) 192, 1585
PaeR7I CTCGAG 1 cut(s) 1721
PciI ACATGT 1 cut(s) 188
PciSI GCTCTTC 3 cut(s) 1090, 2534, 2567
PctI GAATGC 1 cut(s) 2500
PdiI GCCGGC 1 cut(s) 25
PdmI GAANNNNTTC 1 cut(s) 2543
PfeI GAWTC 7 cut(s) 367, 727, 971, 1280, 1386, 2198, 2747
PflMI CCANNNNNTGG 1 cut(s) 1245
PfoI TCCNGGA 1 cut(s) 732
PkrI GCNGC 8 cut(s) 13, 16, 78, 466, 763, 881, 1530, 1533
PleI GAGTC 6 cut(s) 600, 685, 1631, 1732, 2145, 2331
PpsI GAGTC 6 cut(s) 600, 685, 1631, 1732, 2145, 2331
PscI ACATGT 1 cut(s) 188
Psp6I CCWGG 2 cut(s) 1492, 1774
PspFI CCCAGC 1 cut(s) 1400
PspGI CCWGG 2 cut(s) 1492, 1774
PspN4I GGNNCC 6 cut(s) 210, 980, 1691, 2018, 2338, 2362
PspPI GGNCC 1 cut(s) 978
PspXI VCTCGAGB 1 cut(s) 1721
PstI CTGCAG 2 cut(s) 16, 1536
PstNI CAGNNNCTG 1 cut(s) 2388
PsuI RGATCY 2 cut(s) 1741, 2036
RsaI GTAC 4 cut(s) 1599, 1751, 2409, 2470
RsaNI GTAC 4 cut(s) 1598, 1750, 2408, 2469
RseI CAYNNNNRTG 2 cut(s) 2139, 2163
SapI GCTCTTC 3 cut(s) 1090, 2534, 2567
SatI GCNGC 8 cut(s) 12, 15, 77, 465, 762, 880, 1529, 1532
Sau96I GGNCC 1 cut(s) 978
ScaI AGTACT 1 cut(s) 1599
SchI GAGTC 6 cut(s) 600, 686, 1631, 1733, 2145, 2332
ScrFI CCNGG 3 cut(s) 734, 1494, 1776
SduI GDGCHC 3 cut(s) 154, 1669, 2365
SexAI ACCWGGT 1 cut(s) 1774
SfaNI GCATC 7 cut(s) 403, 438, 1294, 1430, 1767, 2247, 2621
SfcI CTRYAG 2 cut(s) 12, 1532
Sfr274I CTCGAG 1 cut(s) 1721
SinI GGWCC 1 cut(s) 978
SlaI CTCGAG 1 cut(s) 1721
SmiMI CAYNNNNRTG 2 cut(s) 2139, 2163
SmlI CTYRAG 4 cut(s) 1102, 1721, 1799, 2275
SmoI CTYRAG 4 cut(s) 1102, 1721, 1799, 2275
SsiI CCGC 3 cut(s) 161, 564, 2026
SspI AATATT 3 cut(s) 1193, 1948, 2623
SspMI CTAG 7 cut(s) 900, 1076, 1712, 1871, 2769, 2808, 2812
StyD4I CCNGG 3 cut(s) 732, 1492, 1774
StyI CCWWGG 3 cut(s) 837, 2261, 2417
TaaI ACNGT 6 cut(s) 402, 1033, 1090, 1835, 1851, 2390
TaqI TCGA 9 cut(s) 131, 334, 919, 1123, 1384, 1443, 1722, 2176, 2504
TatI WGTACW 3 cut(s) 1597, 2407, 2468
TfiI GAWTC 7 cut(s) 367, 727, 971, 1280, 1386, 2198, 2747
TseFI GTSAC 2 cut(s) 457, 661
TseI GCWGC 8 cut(s) 11, 14, 76, 464, 761, 879, 1528, 1531
Tsp45I GTSAC 2 cut(s) 457, 661
TspDTI ATGAA 9 cut(s) 353, 492, 546, 1491, 1496, 1796, 1941, 2157, 2688
Van91I CCANNNNNTGG 1 cut(s) 1245
Vha464I CTTAAG 2 cut(s) 1799, 2275
VneI GTGCAC 1 cut(s) 1665
VpaK11BI GGWCC 1 cut(s) 978
XapI RAATTY 5 cut(s) 852, 1058, 1620, 1904, 1967
XceI RCATGY 2 cut(s) 192, 1585
XhoI CTCGAG 1 cut(s) 1721
XmiI GTMKAC 2 cut(s) 1630, 2251
XmnI GAANNNNTTC 1 cut(s) 2543
XspI CTAG 7 cut(s) 900, 1076, 1712, 1871, 2769, 2808, 2812
ZrmI AGTACT 1 cut(s) 1599
Zsp2I ATGCAT 2 cut(s) 418, 1338
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.