RchiOBHm_Chr6g0258471

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0011520
Physical Location & Seq
Forward (+)
13771758 .. 13775160
3403 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23174

Sequence Viewer

Length: 2934 bp
ATGGAGTTGGCTGCAGCCATTGCCGGCATTTTGAGTTGTGCATGTCCAAAAAATGTTATACAAGAAACAACAGGGCAGCATTGCAAGTATCTTCATTACAATGCAAAAGCAAAGATAGATGAGTTGAGGTCGAAAATGGAGGAATTAAGAGCACTTGTGGCGGATGTAAAAAGAAAACTGGAGGATACATGTCTCCAAGAAGGAAAGGAACCCACAGAACAGGTGAAGTTGTGGTTGAGACATGTTCAAAGCTTTGATAATGAAAAGCTGAATAAGCTACTATGTTGGGAAGAGGAAAGATGTTTATGTGGGTGTCTTCCAGATTATTGCTCTCGAATGAAGTTGGGGAAACTTATCGAAAGGAGGATTCAGAAGGTGGATGAGCTTCTCAGAAGAGGACAGTTTCAAGATGATGCATTGGCTCACTTGTTGTTAGAGAATATAAAGATGCTTCCTATGACAGTAGCGGTGGGTCATGAAACAACTAAAAGAAAGTTGGAAGAGATATGGGAGCATTTAATGGCAGGTTATGAAGTGCAAAGAATTGGTGTTTATGGAATGGGTGGGGTTGGAAAGACAACAATTCTGAAAGAAATAAATAACAGACTCAAAAAAGAGATGACAGAAGGGATGATCACTTGCTTTGACAATGTTGTTTGGGTCACAATACCAAAGGAGTCCAACCCCGAGAAATTGCAAATGGCGATTGCCAAAGCTGTGAGGTTGAATCTCCCTGAAGACTGGAATATGATGGACAAGGCAGCAGAGTTGCTGAAATCATTACAACGAAGGAAGAGGCTTTTGCTAATTTTTGATGATGTATGGACACCATTTTCATTGGAAGATATTGGGATTCCTGCACCTACCAGGGCCAATGGCTGCAAGCTCATATTAACTACTAGATCATTAAAGGTGTGTCGAGGCATGGAGACAAACAAAGAAGTGGAGGTAAAAGTGCTTTCTGAGGAAGAATCTTGGACCCTTTTCACTGATAAAGTTGGTTCTGTTGTGTTACAAAATCCCGAAATTAAAATTATTGCAAGGGAAATAGCAAAAGAATTTGGTGGTCTTCCACTAGCCATTATCACAGTTGGAAGAGCCTTGAGAAAAGTAAGTAATGAAATTGAGTGGCAAAATGCATTGACAGCCTTAAGATGTTCTGCAGCTAATGTGGAAGGCATGGAAGAGCTGGTGTATTCTCGTTTGAGATTCAGTTATGAAAGATTAAAGGATGACACTACTCGATCATGTTTTCTGTATTGTGCATTGTATCCTGAAGATCATCAAATTGAAGCCAAAGAGCTGATAGAATATTGGATGTGGGAGGGTTTGCTTGGATGTGGGGGAAGGATAGAGGCCAATATATGGAAGGGAGAAATGATTTTGGCTGATCTTAAAGATTCTTGCCTGCTGGACAATGTTGATGTAGATGGCGGTGTTGAATGTGTCAAAATGCATGACTTAATAAGGGATATGGCTATTGGTATTACAAGTATGAGTCCTCGATTCATCATTAGTGCTGGGATTGGAGTAGAAAAGGCATCACTTGGGGAGGAGGGGATTGAAGATGTTGAGAGGATTTCAATGATGTATAATGAATTGAAATGCCTTCCTAGCCAACCATTGTGTTCCAAGCTCTCCTCTTTGCTGCTGCAGTATAGCTCTATGGCCAATGATATCTCGCATCGTTTCTTCTGCCACATGCCCAAGCTCAGAGTACTTGATCTGTCTTTCACTGGAATTCTTTGTATACCTGACTCCATTGCTGAGCTAAGCAATTTGCGTGCACTGCTATTGCGTAGTTGTTGGAACCTAAGTAGTCTTCCTTCTCTAGTAACACTCGAGGCATTGTGGGTTTTGGATCTTTCATATACTCACATCACAGAATTGCCTCTAGGTATGGAAAAGTTACGCCAGCTTAGGCGCCTCAATCTTTCTTATACTAAATTGGACAGTTTTCCGAAAGGACTGTTAACCACTCTCAATCTGCTAGAAGAATTGCTCCTATATCGTTGTAAGTGTGAATTTGGTTTACAGTTTGTTGATGAACTCATCACCTCAAGAAATATTGTTGTTCTTGAAGTCGAATTCTCAACCTCCAGAGTGTTTGATACGTATTCAAGATCAGGGCATTGGAATCTTCTGCGGAGTTTTAGATCCGGCATATGCTCATATGGCGATGACTTGGGAAAGAACAGTATAGACTACATTGGAAATTTTTTTCTCAGTGGTACTCCTGTTTTGCTCCCTGATCGCACCTATGAACTGCGACTCCTCCACTGCTATGATGTTGATCGATTGTCAAGGTGCTTGTCAGAATCAATGAAGTTGAAAAAATGTGTTATAACACACTGCGATCAAATGGAGAGTATAATAATGACTGGGGAGAACAACTTAAGCTGTCTAAATACGTTAGAGCTTGGTTTCCTACAAAACTTTAAGAGTCTCTACAAGGGTGTCATGCCCTCAGGAGCTCTTGGTGCCCTCAAGATACTGCAAATCAGTTATTGTAACTCTTTAGAGAGCTTACTCAACCCAAGGCTGATTGAACACCTCAGAAACCTCGAAGAACTTTCTGTTGAACATTGTCCAAAATTGGAGGTGGTGATAGAGGAGGAGCAGTCGATCCACAATGCTGAAGTCAGGCTTTCAAGATTGAAGAGCTTAAAGTTGTGTGATTTAAGGGAACTGGAGAGCATTTATAGTGAAGAAGTTATTTGCATATCTCTATGCACCATCACTATTGAGGGATGCAATAAACTGAGGAGACTTCCTTTTAGGATTTCCATAGATGAAGGATATCAGCAAAGACAACCATCAATCACTCCCCCCATAAGAAAAATTAAAACCGAGCAAGTGTGGTGGGATTCAGTGGAGTTGGCTAATCCCATTGTAAAGACCTTCCTTCAACATAGGCTGGAGTTGCCTAGCTAG

Protein Analysis

977

Amino Acids

111.58

Weight (kDa)

5.93

Isoelectric Point (pI)

52.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TniB PF05621 162 - 274 2.8e-06 Bacterial TniB protein
NB-ARC PF00931 164 - 334 1.6e-41 NB-ARC domain
WHD_DRP PF23559 423 - 493 8.6e-11 Disease resistance protein Winged helix domain
LRR_8 PF13855 545 - 602 6.5e-06 Leucine rich repeat
LRR_14 PF23598 613 - 699 7.1e-07 Leucine-rich repeat region
LRR_RPS2 PF23247 818 - 934 3.8e-15 Plant disease resistance protein RPS2-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2345
Acc36I ACCTGC 1 cut(s) 515
AccB1I GGYRCC 2 cut(s) 1923, 2480
AccB7I CCANNNNNTGG 1 cut(s) 1365
AccI GTMKAC 1 cut(s) 1750
AciI CCGC 4 cut(s) 161, 467, 1434, 2146
AclWI GGATC 3 cut(s) 1869, 2151, 2620
AcoI YGGCCR 1 cut(s) 1668
AcsI RAATTY 5 cut(s) 1058, 1740, 2024, 2087, 2215
AcuI CTGAAG 3 cut(s) 756, 1296, 2658
AcyI GRCGYC 1 cut(s) 1924
AfaI GTAC 2 cut(s) 1719, 2233
AfiI CCNNNNNNNGG 1 cut(s) 1365
AflII CTTAAG 2 cut(s) 1150, 2395
AflIII ACRYGT 2 cut(s) 188, 241
AjnI CCWGG 1 cut(s) 866
AloI GAACNNNNNNTCC 2 cut(s) 2256, 2288
Alw21I GWGCWC 3 cut(s) 154, 1789, 2476
Alw26I GTCTC 5 cut(s) 197, 232, 923, 2450, 2761
Alw44I GTGCAC 1 cut(s) 1785
AlwI GGATC 3 cut(s) 1869, 2151, 2620
Ama87I CYCGRG 2 cut(s) 686, 1841
AoxI GGCC 3 cut(s) 870, 1356, 1668
ApaLI GTGCAC 1 cut(s) 1785
ApeKI GCWGC 8 cut(s) 11, 14, 76, 761, 879, 1163, 1648, 1651
ApoI RAATTY 5 cut(s) 1058, 1740, 2024, 2087, 2215
AspLEI GCGC 1 cut(s) 1926
AspS9I GGNCC 2 cut(s) 870, 978
AsuHPI GGTGA 3 cut(s) 235, 2047, 2617
AvaI CYCGRG 2 cut(s) 686, 1841
AvaII GGWCC 1 cut(s) 978
AxyI CCTNAGG 1 cut(s) 2467
BaeGI GKGCMC 2 cut(s) 1789, 2485
BaeI ACNNNNGTAYC 2 cut(s) 2223, 2256
BalI TGGCCA 1 cut(s) 1670
BanI GGYRCC 2 cut(s) 1923, 2480
BanII GRGCYC 1 cut(s) 2476
BarI GAAGNNNNNNTAC 2 cut(s) 1810, 1842
BbsI GAAGAC 4 cut(s) 308, 744, 1061, 1814
Bbv12I GWGCWC 3 cut(s) 154, 1789, 2476
BbvI GCAGC 7 cut(s) 26, 88, 773, 866, 1175, 1635, 1638
BccI CCATC 4 cut(s) 745, 1424, 2744, 2824
BciT130I CCWGG 1 cut(s) 868
BciVI GTATCC 2 cut(s) 178, 1281
BclI TGATCA 1 cut(s) 633
BcoDI GTCTC 5 cut(s) 197, 232, 923, 2450, 2761
BfaI CTAG 8 cut(s) 900, 1076, 1614, 1832, 1895, 1991, 2928, 2932
BfmI CTRYAG 3 cut(s) 12, 1161, 1652
BfoI RGCGCY 1 cut(s) 1927
BfrI CTTAAG 2 cut(s) 1150, 2395
BfuAI ACCTGC 1 cut(s) 515
BfuI GTATCC 2 cut(s) 178, 1281
BisI GCNGC 8 cut(s) 12, 15, 77, 762, 880, 1164, 1649, 1652
BlpI GCTNAGC 2 cut(s) 1767, 1772
BlsI GCNGC 8 cut(s) 13, 16, 78, 763, 881, 1165, 1650, 1653
BmcAI AGTACT 1 cut(s) 1719
Bme1390I CCNGG 1 cut(s) 868
Bme18I GGWCC 1 cut(s) 978
BmeT110I CYCGRG 2 cut(s) 686, 1841
BmgT120I GGNCC 2 cut(s) 870, 978
BmiI GGNNCC 5 cut(s) 210, 980, 1811, 1925, 2482
BmrFI CCNGG 1 cut(s) 868
BmrI ACTGGG 1 cut(s) 2391
BmsI GCATC 5 cut(s) 403, 438, 1550, 1693, 2741
BmuI ACTGGG 1 cut(s) 2391
BpiI GAAGAC 4 cut(s) 308, 744, 1061, 1814
BpmI CTGGAG 3 cut(s) 200, 2083, 2711
Bpu10I CCTNAGC 1 cut(s) 1919
Bpu1102I GCTNAGC 2 cut(s) 1767, 1772
BpuEI CTTGAG 3 cut(s) 1123, 2044, 2471
Bsa29I ATCGAT 1 cut(s) 2296
BsaAI YACGTR 1 cut(s) 2115
BsaBI GATNNNNATC 1 cut(s) 2292
BsaHI GRCGYC 1 cut(s) 1924
BsaJI CCNNGG 2 cut(s) 867, 2537
BsaXI ACNNNNNCTCC 4 cut(s) 2256, 2286, 2606, 2636
Bsc4I CCNNNNNNNGG 1 cut(s) 1365
Bse118I RCCGGY 1 cut(s) 23
Bse1I ACTGG 5 cut(s) 183, 746, 1741, 2386, 2694
Bse21I CCTNAGG 1 cut(s) 2467
Bse3DI GCAATG 3 cut(s) 18, 79, 1761
Bse8I GATNNNNATC 1 cut(s) 2292
BseBI CCWGG 1 cut(s) 868
BseCI ATCGAT 1 cut(s) 2296
BseDI CCNNGG 2 cut(s) 867, 2537
BseGI GGATG 7 cut(s) 169, 385, 636, 1237, 1323, 1343, 2756
BseJI GATNNNNATC 1 cut(s) 2292
BseLI CCNNNNNNNGG 1 cut(s) 1365
BseMI GCAATG 3 cut(s) 18, 79, 1761
BseMII CTCAG 8 cut(s) 403, 954, 1726, 1758, 2239, 2481, 2569, 2753
BseNI ACTGG 5 cut(s) 183, 746, 1741, 2386, 2694
BseRI GAGGAG 6 cut(s) 1568, 1630, 2264, 2627, 2630, 2779
BseSI GKGCMC 2 cut(s) 1789, 2485
BseXI GCAGC 7 cut(s) 26, 88, 773, 866, 1175, 1635, 1638
BseYI CCCAGC 1 cut(s) 1520
BsgI GTGCAG 1 cut(s) 843
BshFI GGCC 3 cut(s) 872, 1358, 1670
BshNI GGYRCC 2 cut(s) 1923, 2480
BshVI ATCGAT 1 cut(s) 2296
BsiHKAI GWGCWC 3 cut(s) 154, 1789, 2476
BsiHKCI CYCGRG 2 cut(s) 686, 1841
BsiSI CCGG 2 cut(s) 24, 2160
BslI CCNNNNNNNGG 1 cut(s) 1365
BsmAI GTCTC 5 cut(s) 197, 232, 923, 2450, 2761
BsnI GGCC 3 cut(s) 872, 1358, 1670
BsoBI CYCGRG 2 cut(s) 686, 1841
Bsp1286I GDGCHC 4 cut(s) 154, 1789, 2476, 2485
Bsp1720I GCTNAGC 2 cut(s) 1767, 1772
BspACI CCGC 4 cut(s) 161, 467, 1434, 2146
BspANI GGCC 3 cut(s) 872, 1358, 1670
BspCNI CTCAG 8 cut(s) 402, 955, 1725, 1759, 2238, 2480, 2568, 2754
BspDI ATCGAT 1 cut(s) 2296
BspHI TCATGA 1 cut(s) 475
BspLI GGNNCC 5 cut(s) 210, 980, 1811, 1925, 2482
BspMAI CTGCAG 3 cut(s) 16, 1165, 1656
BspMI ACCTGC 1 cut(s) 515
BspPI GGATC 3 cut(s) 1869, 2151, 2620
BspQI GCTCTTC 3 cut(s) 1090, 1179, 2654
BspT107I GGYRCC 2 cut(s) 1923, 2480
BspTI CTTAAG 2 cut(s) 1150, 2395
BsrDI GCAATG 3 cut(s) 18, 79, 1761
BsrFI RCCGGY 1 cut(s) 23
BsrI ACTGG 5 cut(s) 183, 746, 1741, 2386, 2694
BssAI RCCGGY 1 cut(s) 23
BssECI CCNNGG 2 cut(s) 867, 2537
BssNAI GTATAC 1 cut(s) 1751
BssNI GRCGYC 1 cut(s) 1924
BssT1I CCWWGG 1 cut(s) 2537
Bst1107I GTATAC 1 cut(s) 1751
Bst2UI CCWGG 1 cut(s) 868
Bst4CI ACNGT 7 cut(s) 402, 463, 1090, 1955, 1971, 2037, 2198
Bst6I CTCTTC 7 cut(s) 285, 388, 495, 788, 1090, 1179, 2654
BstACI GRCGYC 1 cut(s) 1924
BstAFI CTTAAG 2 cut(s) 1150, 2395
BstAPI GCANNNNNTGC 1 cut(s) 20
BstBAI YACGTR 1 cut(s) 2115
BstC8I GCNNGC 5 cut(s) 25, 884, 1409, 1785, 1916
BstF5I GGATG 7 cut(s) 169, 385, 636, 1237, 1323, 1343, 2756
BstH2I RGCGCY 1 cut(s) 1927
BstHHI GCGC 1 cut(s) 1926
BstMAI GTCTC 5 cut(s) 197, 232, 923, 2450, 2761
BstMWI GCNNNNNNNGC 9 cut(s) 20, 158, 274, 1145, 1614, 1789, 2175, 2480, 2923
BstNI CCWGG 1 cut(s) 868
BstNSI RCATGY 4 cut(s) 45, 192, 245, 1705
BstSCI CCNGG 1 cut(s) 866
BstSFI CTRYAG 3 cut(s) 12, 1161, 1652
BstSLI GKGCMC 2 cut(s) 1789, 2485
BstSNI TACGTA 1 cut(s) 2115
BstV1I GCAGC 7 cut(s) 26, 88, 773, 866, 1175, 1635, 1638
BstV2I GAAGAC 4 cut(s) 308, 744, 1061, 1814
BstX2I RGATCY 2 cut(s) 1861, 2156
BstYI RGATCY 2 cut(s) 1861, 2156
BstZ17I GTATAC 1 cut(s) 1751
Bsu15I ATCGAT 1 cut(s) 2296
Bsu36I CCTNAGG 1 cut(s) 2467
BsuI GTATCC 2 cut(s) 178, 1281
BsuRI GGCC 3 cut(s) 872, 1358, 1670
BsuTUI ATCGAT 1 cut(s) 2296
BtgZI GCGATG 1 cut(s) 2193
BtsCI GGATG 7 cut(s) 169, 385, 636, 1237, 1323, 1343, 2756
BtsI GCAGTG 3 cut(s) 1787, 2278, 2350
BtsIMutI CAGTG 7 cut(s) 987, 1734, 1787, 2233, 2278, 2350, 2877
BveI ACCTGC 1 cut(s) 515
Cac8I GCNNGC 5 cut(s) 25, 884, 1409, 1785, 1916
CciI TCATGA 1 cut(s) 475
CfoI GCGC 1 cut(s) 1926
Cfr10I RCCGGY 1 cut(s) 23
Cfr13I GGNCC 2 cut(s) 870, 978
ClaI ATCGAT 1 cut(s) 2296
Csp6I GTAC 2 cut(s) 1718, 2232
CspCI CAANNNNNGTGG 2 cut(s) 2843, 2878
CviQI GTAC 2 cut(s) 1718, 2232
DinI GGCGCC 1 cut(s) 1925
EaeI YGGCCR 1 cut(s) 1668
Eam1104I CTCTTC 7 cut(s) 285, 388, 495, 788, 1090, 1179, 2654
EarI CTCTTC 7 cut(s) 285, 388, 495, 788, 1090, 1179, 2654
EciI GGCGGA 1 cut(s) 176
Ecl136II GAGCTC 1 cut(s) 2474
Eco105I TACGTA 1 cut(s) 2115
Eco130I CCWWGG 1 cut(s) 2537
Eco24I GRGCYC 1 cut(s) 2476
Eco32I GATATC 2 cut(s) 1678, 2801
Eco47I GGWCC 1 cut(s) 978
Eco53kI GAGCTC 1 cut(s) 2474
Eco57I CTGAAG 3 cut(s) 756, 1296, 2658
Eco81I CCTNAGG 1 cut(s) 2467
Eco88I CYCGRG 2 cut(s) 686, 1841
EcoICRI GAGCTC 1 cut(s) 2474
EcoRI GAATTC 2 cut(s) 1740, 2087
EcoRII CCWGG 1 cut(s) 866
EcoRV GATATC 2 cut(s) 1678, 2801
EcoT14I CCWWGG 1 cut(s) 2537
EcoT22I ATGCAT 3 cut(s) 418, 1141, 1458
EcoT38I GRGCYC 1 cut(s) 2476
EgeI GGCGCC 1 cut(s) 1925
EheI GGCGCC 1 cut(s) 1925
ErhI CCWWGG 1 cut(s) 2537
FalI AAGNNNNNCTT 2 cut(s) 2631, 2663
FauNDI CATATG 2 cut(s) 2165, 2173
FbaI TGATCA 1 cut(s) 633
FblI GTMKAC 1 cut(s) 1750
Fnu4HI GCNGC 8 cut(s) 12, 15, 77, 762, 880, 1164, 1649, 1652
FokI GGATG 7 cut(s) 176, 392, 643, 1244, 1330, 1350, 2763
FriOI GRGCYC 1 cut(s) 2476
Fsp4HI GCNGC 8 cut(s) 12, 15, 77, 762, 880, 1164, 1649, 1652
FspBI CTAG 8 cut(s) 900, 1076, 1614, 1832, 1895, 1991, 2928, 2932
GlaI GCGC 1 cut(s) 1925
GluI GCNGC 8 cut(s) 12, 15, 77, 762, 880, 1164, 1649, 1652
GsaI CCCAGC 1 cut(s) 1524
GsuI CTGGAG 3 cut(s) 200, 2083, 2711
HaeII RGCGCY 1 cut(s) 1927
HaeIII GGCC 3 cut(s) 872, 1358, 1670
HapII CCGG 2 cut(s) 24, 2160
HhaI GCGC 1 cut(s) 1926
Hin1I GRCGYC 1 cut(s) 1924
Hin6I GCGC 1 cut(s) 1924
HinP1I GCGC 1 cut(s) 1924
HincII GTYRAC 1 cut(s) 1974
HindII GTYRAC 1 cut(s) 1974
HindIII AAGCTT 1 cut(s) 250
HpaI GTTAAC 1 cut(s) 1974
HpaII CCGG 2 cut(s) 24, 2160
HphI GGTGA 3 cut(s) 235, 2047, 2617
Hpy166II GTNNAC 4 cut(s) 1751, 1787, 1974, 2033
Hpy188I TCNGA 8 cut(s) 372, 392, 588, 964, 1715, 1962, 2317, 2558
Hpy8I GTNNAC 4 cut(s) 1751, 1787, 1974, 2033
HpyCH4III ACNGT 7 cut(s) 402, 463, 1090, 1955, 1971, 2037, 2198
HpyCH4IV ACGT 2 cut(s) 2114, 2411
HpyF10VI GCNNNNNNNGC 9 cut(s) 20, 158, 274, 1145, 1614, 1789, 2175, 2480, 2923
HpySE526I ACGT 2 cut(s) 2114, 2411
Hsp92I GRCGYC 1 cut(s) 1924
HspAI GCGC 1 cut(s) 1924
KasI GGCGCC 1 cut(s) 1923
KroI GCCGGC 1 cut(s) 23
KroNI GCCGGC 1 cut(s) 25
Ksp22I TGATCA 1 cut(s) 633
KspAI GTTAAC 1 cut(s) 1974
LguI GCTCTTC 3 cut(s) 1090, 1179, 2654
LmnI GCTCC 5 cut(s) 511, 2007, 2250, 2471, 2617
Lsp1109I GCAGC 7 cut(s) 26, 88, 773, 866, 1175, 1635, 1638
LweI GCATC 5 cut(s) 403, 438, 1550, 1693, 2741
MaeI CTAG 8 cut(s) 900, 1076, 1614, 1832, 1895, 1991, 2928, 2932
MaeII ACGT 2 cut(s) 2114, 2411
MaeIII GTNAC 5 cut(s) 661, 1011, 1834, 1908, 2510
MflI RGATCY 2 cut(s) 1861, 2156
MhlI GDGCHC 4 cut(s) 154, 1789, 2476, 2485
MlsI TGGCCA 1 cut(s) 1670
MluNI TGGCCA 1 cut(s) 1670
Mly113I GGCGCC 1 cut(s) 1924
MlyI GAGTC 6 cut(s) 600, 686, 1507, 1751, 2265, 2452
MmeI TCCRAC 5 cut(s) 477, 550, 705, 1072, 1787
Mox20I TGGCCA 1 cut(s) 1670
Mph1103I ATGCAT 3 cut(s) 418, 1141, 1458
MroNI GCCGGC 1 cut(s) 23
MscI TGGCCA 1 cut(s) 1670
MslI CAYNNNNRTG 2 cut(s) 99, 2283
Msp20I TGGCCA 1 cut(s) 1670
MspCI CTTAAG 2 cut(s) 1150, 2395
MspI CCGG 2 cut(s) 24, 2160
MspR9I CCNGG 1 cut(s) 868
MvaI CCWGG 1 cut(s) 868
MwoI GCNNNNNNNGC 9 cut(s) 20, 158, 274, 1145, 1614, 1789, 2175, 2480, 2923
NaeI GCCGGC 1 cut(s) 25
NarI GGCGCC 1 cut(s) 1924
NdeI CATATG 2 cut(s) 2165, 2173
NgoMIV GCCGGC 1 cut(s) 23
NlaIV GGNNCC 5 cut(s) 210, 980, 1811, 1925, 2482
NmuCI GTSAC 1 cut(s) 661
NsiI ATGCAT 3 cut(s) 418, 1141, 1458
NspI RCATGY 4 cut(s) 45, 192, 245, 1705
PaeR7I CTCGAG 1 cut(s) 1841
PagI TCATGA 1 cut(s) 475
PciI ACATGT 2 cut(s) 188, 241
PciSI GCTCTTC 3 cut(s) 1090, 1179, 2654
PdiI GCCGGC 1 cut(s) 25
PflMI CCANNNNNTGG 1 cut(s) 1365
PkrI GCNGC 8 cut(s) 13, 16, 78, 763, 881, 1165, 1650, 1653
PleI GAGTC 6 cut(s) 600, 685, 1506, 1751, 2265, 2451
PluTI GGCGCC 1 cut(s) 1927
PpsI GAGTC 6 cut(s) 600, 685, 1506, 1751, 2265, 2451
Ppu21I YACGTR 1 cut(s) 2115
PscI ACATGT 2 cut(s) 188, 241
PsiI TTATAA 1 cut(s) 2345
Psp124BI GAGCTC 1 cut(s) 2476
Psp6I CCWGG 1 cut(s) 866
PspFI CCCAGC 1 cut(s) 1520
PspGI CCWGG 1 cut(s) 866
PspN4I GGNNCC 5 cut(s) 210, 980, 1811, 1925, 2482
PspPI GGNCC 2 cut(s) 870, 978
PspXI VCTCGAGB 1 cut(s) 1841
PstI CTGCAG 3 cut(s) 16, 1165, 1656
PsuI RGATCY 2 cut(s) 1861, 2156
RsaI GTAC 2 cut(s) 1719, 2233
RsaNI GTAC 2 cut(s) 1718, 2232
RseI CAYNNNNRTG 2 cut(s) 99, 2283
SacI GAGCTC 1 cut(s) 2476
SapI GCTCTTC 3 cut(s) 1090, 1179, 2654
SatI GCNGC 8 cut(s) 12, 15, 77, 762, 880, 1164, 1649, 1652
Sau96I GGNCC 2 cut(s) 870, 978
ScaI AGTACT 1 cut(s) 1719
SchI GAGTC 6 cut(s) 600, 686, 1507, 1751, 2265, 2452
ScrFI CCNGG 1 cut(s) 868
SduI GDGCHC 4 cut(s) 154, 1789, 2476, 2485
SfaNI GCATC 5 cut(s) 403, 438, 1550, 1693, 2741
SfcI CTRYAG 3 cut(s) 12, 1161, 1652
SfoI GGCGCC 1 cut(s) 1925
Sfr274I CTCGAG 1 cut(s) 1841
SinI GGWCC 1 cut(s) 978
SlaI CTCGAG 1 cut(s) 1841
SmiMI CAYNNNNRTG 2 cut(s) 99, 2283
SmlI CTYRAG 6 cut(s) 1102, 1150, 1841, 2059, 2395, 2486
SmoI CTYRAG 6 cut(s) 1102, 1150, 1841, 2059, 2395, 2486
SnaBI TACGTA 1 cut(s) 2115
SsiI CCGC 4 cut(s) 161, 467, 1434, 2146
SspDI GGCGCC 1 cut(s) 1923
SspI AATATT 2 cut(s) 1313, 2068
SspMI CTAG 8 cut(s) 900, 1076, 1614, 1832, 1895, 1991, 2928, 2932
SstI GAGCTC 1 cut(s) 2476
StyD4I CCNGG 1 cut(s) 866
StyI CCWWGG 1 cut(s) 2537
TaaI ACNGT 7 cut(s) 402, 463, 1090, 1955, 1971, 2037, 2198
TaiI ACGT 2 cut(s) 2117, 2414
TatI WGTACW 1 cut(s) 1717
TscAI CASTG 7 cut(s) 994, 1741, 1794, 2233, 2285, 2357, 2877
TseFI GTSAC 1 cut(s) 661
TseI GCWGC 8 cut(s) 11, 14, 76, 761, 879, 1163, 1648, 1651
Tsp45I GTSAC 1 cut(s) 661
TspRI CASTG 7 cut(s) 994, 1741, 1794, 2233, 2285, 2357, 2877
Van91I CCANNNNNTGG 1 cut(s) 1365
Vha464I CTTAAG 2 cut(s) 1150, 2395
VneI GTGCAC 1 cut(s) 1785
VpaK11BI GGWCC 1 cut(s) 978
XapI RAATTY 5 cut(s) 1058, 1740, 2024, 2087, 2215
XceI RCATGY 4 cut(s) 45, 192, 245, 1705
XhoI CTCGAG 1 cut(s) 1841
XmiI GTMKAC 1 cut(s) 1750
XspI CTAG 8 cut(s) 900, 1076, 1614, 1832, 1895, 1991, 2928, 2932
ZrmI AGTACT 1 cut(s) 1719
Zsp2I ATGCAT 3 cut(s) 418, 1141, 1458
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.