Rorug05G0581900

Serine threonine-protein kinase PBS1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000397
Physical Location & Seq
Forward (+)
77905053 .. 77909717
4665 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0581900.1

Sequence Viewer

Length: 837 bp
ATGGCGCCGAGTCCACCCCTAGATGAATATGATGAAATCAAAAATCAGAAGATGAAGACTAAAAAGTCAAGTAAGAAGCGTGCAGCACTGGAGCTAGATACTCCGGCTATTAAAGACAAGGTTGAAATTGGTCATGGAGAGACTGCAGATGGAGTCCTTGTTGATCAGGATTTCAACGAACCAACCATGGGAGAAAAACTTGCTAGTTTGAATATAGTACCTGATGATAGAGTGGAGAGCCAAAATAAAGAAGGCTCTTCTCTCCATGCAAAGCCTCCGAGTGCAGACTCTGTTCATATTTTGCTTAAGCAAGCACTACATGCTGATGACCGTGCCCTTCTATTCGAGTGCTTATACACCCAAGATGAGAAGGTCATTGCAAAGTCTGTCTCTCAATTGAATCCAGCTGGTGTTTTGAAGCTCTTACAATCTCTCATATCTCTTATTGAGTCAAGGGGTGCAATTTTGGCATGTGCACTTCCATGGCTGAGAAGTCTACTTCTTCAACACGCAAGTGGGATAGTGTCTCAAGCATCTTCTTTAAGTGCCTTGAACTCTTTATATCAGCTCATTGAGTCCAGAGTTTCCACTTTTCAGTCAGCTCTTCAACTATCAAGTGTTTTAGACTTGCTGTATACAGGGGTTGTTGATGATTTGGATGATGAGAATGGCACTACAGGTCCAGTTATTTACGAGGACAAAGATAGTGATGAAGAGGAATCTGAGGACGCCATGGAGACTGATCAAGACGATGAAGATGGTGAGGATTTGGATGCAGAACTTGGTGGTGTTAGCGACTTCGAAGGAATTGAAGGACTTGATGACTTGAGCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

278

Amino Acids

30.16

Weight (kDa)

4.23

Isoelectric Point (pI)

54.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Utp12 PF04003 110 - 211 1.2e-16 Dip2/Utp12 Family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48690 AT3G48700
fragaria_vesca FvH4_2g06400 FvH4_2g06430 FvH4_2g06450 FvH4_2g06480 FvH4_2g06560
malus_domestica MD05G1076400.v1.1 MD05G1076500.v1.1 MD05G1078900.v1.1 MD05G1191000.v1.1 MD05G1191100.v1.1 MD08G1226300.v1.1 MD10G1091000.v1.1 MD10G1091200.v1.1 MD10G1091400.v1.1 MD10G1091600.v1.1 MD10G1091900.v1.1
prunus_persica Prupe.8G120800_v2.0.a1 Prupe.8G121100_v2.0.a1 Prupe.8G121200_v2.0.a1 Prupe.8G121300_v2.0.a1 Prupe.8G121400_v2.0.a1 Prupe.8G121500_v2.0.a1 Prupe.8G121600_v2.0.a1 Prupe.8G121700_v2.0.a1 Prupe.8G121900_v2.0.a1 Prupe.8G122000_v2.0.a1 Prupe.I000800_v2.0.a1
pyrus_communis pycom05g07040 pycom05g17560 pycom05g17680 pycom05g17760 pycom10g07450 pycom10g07470 pycom10g07550
rosa_chinensis RchiOBHm_Chr6g0258271 RchiOBHm_Chr6g0258281 RchiOBHm_Chr6g0258291 RchiOBHm_Chr6g0258311 RchiOBHm_Chr6g0258321 RchiOBHm_Chr6g0258501
rosa_laevigata RLG00000014606 RLG00000014621 RLG00000014622 RLG00000014624 RLG00000014625 RLG00000014627 RLG00000014629 RLG00000014633 RLG00000014635
rosa_multiflora Rmu_co8014592.1_g000001 Rmu_co8366243.1_g000001 Rmu_sc0001663.1_g000004 Rmu_sc0005877.1_g000001 Rmu_sc0005877.1_g000006 Rmu_sc0005877.1_g000007 Rmu_sc0007367.1_g000009 Rmu_sc0007367.1_g000019 Rmu_sc0010616.1_g000022 Rmu_sc0010616.1_g000023 Rmu_ssc0000213.1_g000009
rosa_roxburghii Rroxscaffold_178G00437230 Rroxscaffold_178G00437250 Rroxscaffold_178G00437280 Rroxscaffold_178G00437320 Rroxscaffold_178G00437340 Rroxscaffold_178G00437350 Rroxscaffold_178G00437480 Rroxscaffold_7G00205390 Rroxscaffold_7G00205410 Rroxscaffold_7G00205450 Rroxscaffold_7G00205490 Rroxscaffold_7G00205510 Rroxscaffold_7G00205520 Rroxscaffold_7G00205670 Rroxscaffold_7G00205730
rosa_rugosa Rorug05G0579800 Rorug05G0580500 Rorug05G0580600 Rorug05G0580700 Rorug05G0580900 Rorug05G0581000 Rorug05G0581900
rosa_samantha Rh6CG084800 Rh6CG085100 Rh6CG085200 Rh6CG085400 Rh6CG085500 Rh6CG085600 Rh6CG085700 Rh6CG086900
rosa_wichuraiana Rw6G008300 Rw6G008310 Rw6G008360 Rw6G008370 Rw6G008380 Rw6G008390 Rw6G008430 Rw6G008440 Rw6G008530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 64
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 2 cut(s) 496, 635
AcyI GRCGYC 2 cut(s) 5, 729
AfaI GTAC 1 cut(s) 219
AfiI CCNNNNNNNGG 1 cut(s) 188
AflII CTTAAG 1 cut(s) 305
AgsI TTSAA 9 cut(s) 125, 175, 211, 400, 418, 506, 553, 608, 812
AleI CACNNNNGTG 1 cut(s) 513
AluBI AGCT 5 cut(s) 94, 407, 421, 568, 602
AluI AGCT 5 cut(s) 94, 407, 421, 568, 602
Alw21I GWGCWC 1 cut(s) 478
Alw26I GTCTC 4 cut(s) 134, 394, 531, 731
Alw44I GTGCAC 1 cut(s) 474
AlwNI CAGNNNCTG 1 cut(s) 290
ApaLI GTGCAC 1 cut(s) 474
ApeKI GCWGC 1 cut(s) 83
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 680
AsuHPI GGTGA 1 cut(s) 773
AsuII TTCGAA 1 cut(s) 801
AvaII GGWCC 1 cut(s) 680
BaeGI GKGCMC 2 cut(s) 337, 478
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 62
Bbv12I GWGCWC 1 cut(s) 478
BbvI GCAGC 1 cut(s) 95
BccI CCATC 2 cut(s) 143, 752
BclI TGATCA 2 cut(s) 163, 742
BcoDI GTCTC 4 cut(s) 134, 394, 531, 731
BfaI CTAG 3 cut(s) 20, 95, 204
BfmI CTRYAG 2 cut(s) 144, 675
BfoI RGCGCY 1 cut(s) 8
BfrI CTTAAG 1 cut(s) 305
BisI GCNGC 1 cut(s) 84
BlsI GCNGC 1 cut(s) 85
Bme18I GGWCC 1 cut(s) 680
BmgT120I GGNCC 1 cut(s) 680
BmiI GGNNCC 1 cut(s) 6
BmsI GCATC 2 cut(s) 542, 763
BpiI GAAGAC 1 cut(s) 62
BpmI CTGGAG 1 cut(s) 110
Bpu14I TTCGAA 1 cut(s) 801
BpuEI CTTGAG 1 cut(s) 513
BsaHI GRCGYC 2 cut(s) 5, 729
BsaJI CCNNGG 3 cut(s) 186, 482, 732
BsaXI ACNNNNNCTCC 2 cut(s) 144, 174
Bsc4I CCNNNNNNNGG 1 cut(s) 188
Bse1I ACTGG 2 cut(s) 93, 683
Bse3DI GCAATG 1 cut(s) 375
BseDI CCNNGG 3 cut(s) 186, 482, 732
BseGI GGATG 2 cut(s) 664, 778
BseLI CCNNNNNNNGG 1 cut(s) 188
BseMI GCAATG 1 cut(s) 375
BseMII CTCAG 2 cut(s) 479, 714
BseNI ACTGG 2 cut(s) 93, 683
BseSI GKGCMC 2 cut(s) 337, 478
BseXI GCAGC 1 cut(s) 95
BsgI GTGCAG 2 cut(s) 102, 303
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 1 cut(s) 478
BsiSI CCGG 1 cut(s) 104
BslI CCNNNNNNNGG 1 cut(s) 188
BsmAI GTCTC 4 cut(s) 134, 394, 531, 731
Bsp119I TTCGAA 1 cut(s) 801
Bsp1286I GDGCHC 2 cut(s) 337, 478
Bsp143I GATC 2 cut(s) 163, 742
Bsp19I CCATGG 3 cut(s) 186, 482, 732
BspCNI CTCAG 2 cut(s) 480, 715
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 148
BspQI GCTCTTC 2 cut(s) 262, 609
BspT104I TTCGAA 1 cut(s) 801
BspT107I GGYRCC 1 cut(s) 4
BspTI CTTAAG 1 cut(s) 305
BsrDI GCAATG 1 cut(s) 375
BsrI ACTGG 2 cut(s) 93, 683
BssECI CCNNGG 3 cut(s) 186, 482, 732
BssMI GATC 2 cut(s) 163, 742
BssNAI GTATAC 1 cut(s) 636
BssNI GRCGYC 2 cut(s) 5, 729
BssT1I CCWWGG 3 cut(s) 186, 482, 732
Bst1107I GTATAC 1 cut(s) 636
Bst4CI ACNGT 1 cut(s) 332
Bst6I CTCTTC 3 cut(s) 262, 609, 708
BstACI GRCGYC 2 cut(s) 5, 729
BstAFI CTTAAG 1 cut(s) 305
BstAPI GCANNNNNTGC 1 cut(s) 320
BstBI TTCGAA 1 cut(s) 801
BstC8I GCNNGC 2 cut(s) 81, 312
BstDEI CTNAG 2 cut(s) 488, 723
BstDSI CCRYGG 3 cut(s) 186, 482, 732
BstF5I GGATG 2 cut(s) 664, 778
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 2 cut(s) 166, 745
BstMAI GTCTC 4 cut(s) 134, 394, 531, 731
BstMBI GATC 2 cut(s) 163, 742
BstMWI GCNNNNNNNGC 2 cut(s) 320, 467
BstNSI RCATGY 2 cut(s) 323, 474
BstSFI CTRYAG 2 cut(s) 144, 675
BstSLI GKGCMC 2 cut(s) 337, 478
BstV1I GCAGC 1 cut(s) 95
BstV2I GAAGAC 1 cut(s) 62
BstZ17I GTATAC 1 cut(s) 636
BtgI CCRYGG 3 cut(s) 186, 482, 732
BtsCI GGATG 2 cut(s) 664, 778
BtsIMutI CAGTG 1 cut(s) 86
Cac8I GCNNGC 2 cut(s) 81, 312
CaiI CAGNNNCTG 1 cut(s) 290
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 1 cut(s) 680
CseI GACGC 1 cut(s) 737
Csp6I GTAC 1 cut(s) 218
CviAII CATG 7 cut(s) 134, 187, 266, 320, 471, 483, 733
CviQI GTAC 1 cut(s) 218
DdeI CTNAG 2 cut(s) 488, 723
DinI GGCGCC 1 cut(s) 6
DpnI GATC 2 cut(s) 165, 744
DpnII GATC 2 cut(s) 163, 742
DrdI GACNNNNNNGTC 1 cut(s) 64
DseDI GACNNNNNNGTC 1 cut(s) 64
Eam1104I CTCTTC 3 cut(s) 262, 609, 708
EarI CTCTTC 3 cut(s) 262, 609, 708
Eco130I CCWWGG 3 cut(s) 186, 482, 732
Eco47I GGWCC 1 cut(s) 680
EcoT14I CCWWGG 3 cut(s) 186, 482, 732
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 3 cut(s) 186, 482, 732
FaeI CATG 7 cut(s) 137, 190, 269, 323, 474, 486, 736
FatI CATG 7 cut(s) 133, 186, 265, 319, 470, 482, 732
FbaI TGATCA 2 cut(s) 163, 742
FblI GTMKAC 2 cut(s) 496, 635
Fnu4HI GCNGC 1 cut(s) 84
FokI GGATG 2 cut(s) 671, 785
Fsp4HI GCNGC 1 cut(s) 84
FspBI CTAG 3 cut(s) 20, 95, 204
GlaI GCGC 1 cut(s) 6
GluI GCNGC 1 cut(s) 84
GsuI CTGGAG 1 cut(s) 110
HaeII RGCGCY 1 cut(s) 8
HapII CCGG 1 cut(s) 104
HgaI GACGC 1 cut(s) 737
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 2 cut(s) 5, 729
Hin1II CATG 7 cut(s) 137, 190, 269, 323, 474, 486, 736
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HinfI GANTC 7 cut(s) 10, 153, 287, 400, 449, 575, 719
HpaII CCGG 1 cut(s) 104
HphI GGTGA 1 cut(s) 773
Hpy166II GTNNAC 4 cut(s) 14, 476, 497, 636
Hpy188I TCNGA 3 cut(s) 48, 279, 724
Hpy188III TCNNGA 3 cut(s) 167, 579, 746
Hpy8I GTNNAC 4 cut(s) 14, 476, 497, 636
HpyAV CCTTC 5 cut(s) 245, 347, 364, 797, 806
HpyCH4III ACNGT 1 cut(s) 332
HpyCH4V TGCA 8 cut(s) 83, 146, 269, 284, 380, 461, 476, 776
HpyF10VI GCNNNNNNNGC 2 cut(s) 320, 467
HpyF3I CTNAG 2 cut(s) 488, 723
Hsp92I GRCGYC 2 cut(s) 5, 729
Hsp92II CATG 7 cut(s) 137, 190, 269, 323, 474, 486, 736
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
Ksp22I TGATCA 2 cut(s) 163, 742
Kzo9I GATC 2 cut(s) 163, 742
LguI GCTCTTC 2 cut(s) 262, 609
LmnI GCTCC 1 cut(s) 91
Lsp1109I GCAGC 1 cut(s) 95
LweI GCATC 2 cut(s) 542, 763
MaeI CTAG 3 cut(s) 20, 95, 204
MalI GATC 2 cut(s) 165, 744
MboI GATC 2 cut(s) 163, 742
MboII GAAGA 8 cut(s) 61, 67, 249, 494, 528, 596, 725, 767
MfeI CAATTG 1 cut(s) 395
MhlI GDGCHC 2 cut(s) 337, 478
MluCI AATT 4 cut(s) 126, 395, 462, 807
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 5 cut(s) 19, 162, 281, 458, 584
MnlI CCTC 5 cut(s) 285, 688, 709, 718, 757
MseI TTAA 3 cut(s) 111, 306, 542
MslI CAYNNNNRTG 3 cut(s) 324, 481, 513
MspA1I CMGCKG 1 cut(s) 407
MspCI CTTAAG 1 cut(s) 305
MspI CCGG 1 cut(s) 104
MunI CAATTG 1 cut(s) 395
MwoI GCNNNNNNNGC 2 cut(s) 320, 467
NarI GGCGCC 1 cut(s) 5
NcoI CCATGG 3 cut(s) 186, 482, 732
NdeII GATC 2 cut(s) 163, 742
NlaIII CATG 7 cut(s) 137, 190, 269, 323, 474, 486, 736
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 1 cut(s) 33
NspI RCATGY 2 cut(s) 323, 474
NspV TTCGAA 1 cut(s) 801
OliI CACNNNNGTG 1 cut(s) 513
PciSI GCTCTTC 2 cut(s) 262, 609
PfeI GAWTC 2 cut(s) 400, 719
PkrI GCNGC 1 cut(s) 85
PleI GAGTC 5 cut(s) 18, 161, 281, 457, 583
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 5 cut(s) 18, 161, 281, 457, 583
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 1 cut(s) 680
PstI CTGCAG 1 cut(s) 148
PstNI CAGNNNCTG 1 cut(s) 290
PvuII CAGCTG 1 cut(s) 407
RsaI GTAC 1 cut(s) 219
RsaNI GTAC 1 cut(s) 218
RseI CAYNNNNRTG 3 cut(s) 324, 481, 513
SapI GCTCTTC 2 cut(s) 262, 609
SaqAI TTAA 3 cut(s) 111, 306, 542
SatI GCNGC 1 cut(s) 84
Sau3AI GATC 2 cut(s) 163, 742
Sau96I GGNCC 1 cut(s) 680
SchI GAGTC 5 cut(s) 19, 162, 281, 458, 584
SduI GDGCHC 2 cut(s) 337, 478
SetI ASST 9 cut(s) 96, 123, 223, 375, 409, 423, 570, 604, 682
SfaNI GCATC 2 cut(s) 542, 763
SfcI CTRYAG 2 cut(s) 144, 675
SfoI GGCGCC 1 cut(s) 6
SfuI TTCGAA 1 cut(s) 801
SinI GGWCC 1 cut(s) 680
SmiMI CAYNNNNRTG 3 cut(s) 324, 481, 513
SmlI CTYRAG 3 cut(s) 305, 528, 826
SmoI CTYRAG 3 cut(s) 305, 528, 826
Sse9I AATT 4 cut(s) 126, 395, 462, 807
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 3 cut(s) 20, 95, 204
StyI CCWWGG 3 cut(s) 186, 482, 732
TaaI ACNGT 1 cut(s) 332
TaqI TCGA 2 cut(s) 345, 801
TasI AATT 4 cut(s) 126, 395, 462, 807
TfiI GAWTC 2 cut(s) 400, 719
Tru1I TTAA 3 cut(s) 111, 306, 542
Tru9I TTAA 3 cut(s) 111, 306, 542
TscAI CASTG 1 cut(s) 93
TseI GCWGC 1 cut(s) 83
TspDTI ATGAA 6 cut(s) 39, 48, 68, 284, 726, 768
TspRI CASTG 1 cut(s) 93
Vha464I CTTAAG 1 cut(s) 305
VneI GTGCAC 1 cut(s) 474
VpaK11BI GGWCC 1 cut(s) 680
XceI RCATGY 2 cut(s) 323, 474
XmiI GTMKAC 2 cut(s) 496, 635
XspI CTAG 3 cut(s) 20, 95, 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.