RchiOBHm_Chr6g0258311

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0000397
Physical Location & Seq
Forward (+)
13690283 .. 13691287
1005 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ23163

Sequence Viewer

Length: 1005 bp
ATGCAGGCCTCGCTACATTGTCGTACTGAGATTATCATTCTCTTCTCTTCCACGAATCCAAACATGAGCGATGAGTTAGCCCATGACTTCTCTCCTCTCTTTAAAATCTACAAAGATGGTCGAATTGAGCGACTCATGGGCACAGATACAGTTCCTCCATCAGTTCATCCCCAAACTGGTGTCGAATCCAAACACGTCATGATTTCAAACGAAACAGGATTATATGCTAGGCTTTACATCCCCAAATCCACCACAACCTCTTCCACCAAACTCCCTCTTCTTGTTTACTTCCACGGCGGCGGCTTCTGTGTTGAAACTGCCTCTTCTCCTACGTACCACAACTACCTCAACTCCTTAGTAGCGGAAGCTAATGTTGTTGCTGTGTCTGTTGACTTTAGGAGAGTCCCAGAATACCCTCTGCCTGTTGCCTACAATGATTCCTGGGATGCTCTCAAATGGGTTGCTTCTCATTCTGATGGAAGTGGCTCTGAGGAGTGGCTAAATAACCATGCAGATTTGCAGAAACTGTTCTTCTCTGGTGATAGTTCTGGTGGTAATATAGCGCATAACATGGCTGTGAAAGTCGGCTCTGAGGGCTTGGTTGGTGTTAAGCTCATAGGGATTGTGCTGGTGCATCCCTTTCTTTGGGGGCAAGAACCAATTGGGGGAGAGTCAACTATGACTGCAGTTCAAAGAGAGTATCTGGATTCTATGTGGCGTTTTGTGTACCCTTTGACTAGCGGATCTGATGATCCGCTTCTCAATCCGGGTAAGGATCCGAAATTGGGTGGTTTGGGGTGTGAGAAAGTGTTGGTTTGTGTTGCTGAGAATGATACATTGAGACATAGAAACTGGTATTACAGTGAGGTCCTTAGAAAGAGTGGATGGAAAGGCGCTGTGGAGGTCTTGGAAGCAAAGGGGGAGGGCCATGTTTTCCATTTGTTCAATCCAACTTGTGACAGTGCTCAGGCCATGCTGAAAAAGATCACTTCTTTCATCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

334

Amino Acids

36.84

Weight (kDa)

5.89

Isoelectric Point (pI)

33.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 62 - 133 3.2e-09 Carboxylesterase family
BD-FAE PF20434 79 - 195 1.6e-10 BD-FAE
Abhydrolase_3 PF07859 94 - 313 1.1e-52 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48690 AT3G48700
fragaria_vesca FvH4_2g06400 FvH4_2g06430 FvH4_2g06450 FvH4_2g06480 FvH4_2g06560
malus_domestica MD05G1076400.v1.1 MD05G1076500.v1.1 MD05G1078900.v1.1 MD05G1191000.v1.1 MD05G1191100.v1.1 MD08G1226300.v1.1 MD10G1091000.v1.1 MD10G1091200.v1.1 MD10G1091400.v1.1 MD10G1091600.v1.1 MD10G1091900.v1.1
prunus_persica Prupe.8G120800_v2.0.a1 Prupe.8G121100_v2.0.a1 Prupe.8G121200_v2.0.a1 Prupe.8G121300_v2.0.a1 Prupe.8G121400_v2.0.a1 Prupe.8G121500_v2.0.a1 Prupe.8G121600_v2.0.a1 Prupe.8G121700_v2.0.a1 Prupe.8G121900_v2.0.a1 Prupe.8G122000_v2.0.a1 Prupe.I000800_v2.0.a1
pyrus_communis pycom05g07040 pycom05g17560 pycom05g17680 pycom05g17760 pycom10g07450 pycom10g07470 pycom10g07550
rosa_chinensis RchiOBHm_Chr6g0258271 RchiOBHm_Chr6g0258281 RchiOBHm_Chr6g0258291 RchiOBHm_Chr6g0258311 RchiOBHm_Chr6g0258321 RchiOBHm_Chr6g0258501
rosa_laevigata RLG00000014606 RLG00000014621 RLG00000014622 RLG00000014624 RLG00000014625 RLG00000014627 RLG00000014629 RLG00000014633 RLG00000014635
rosa_multiflora Rmu_co8014592.1_g000001 Rmu_co8366243.1_g000001 Rmu_sc0001663.1_g000004 Rmu_sc0005877.1_g000001 Rmu_sc0005877.1_g000006 Rmu_sc0005877.1_g000007 Rmu_sc0007367.1_g000009 Rmu_sc0007367.1_g000019 Rmu_sc0010616.1_g000022 Rmu_sc0010616.1_g000023 Rmu_ssc0000213.1_g000009
rosa_roxburghii Rroxscaffold_178G00437230 Rroxscaffold_178G00437250 Rroxscaffold_178G00437280 Rroxscaffold_178G00437320 Rroxscaffold_178G00437340 Rroxscaffold_178G00437350 Rroxscaffold_178G00437480 Rroxscaffold_7G00205390 Rroxscaffold_7G00205410 Rroxscaffold_7G00205450 Rroxscaffold_7G00205490 Rroxscaffold_7G00205510 Rroxscaffold_7G00205520 Rroxscaffold_7G00205670 Rroxscaffold_7G00205730
rosa_rugosa Rorug05G0579800 Rorug05G0580500 Rorug05G0580600 Rorug05G0580700 Rorug05G0580900 Rorug05G0581000 Rorug05G0581900
rosa_samantha Rh6CG084800 Rh6CG085100 Rh6CG085200 Rh6CG085400 Rh6CG085500 Rh6CG085600 Rh6CG085700 Rh6CG086900
rosa_wichuraiana Rw6G008300 Rw6G008310 Rw6G008360 Rw6G008370 Rw6G008380 Rw6G008390 Rw6G008430 Rw6G008440 Rw6G008530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 297, 300, 362, 741, 755
AclWI GGATC 4 cut(s) 746, 751, 770, 783
AfaI GTAC 3 cut(s) 25, 335, 728
AfiI CCNNNNNNNGG 4 cut(s) 176, 645, 665, 785
AflIII ACRYGT 1 cut(s) 193
AgsI TTSAA 4 cut(s) 207, 314, 692, 946
AjiI CACGTC 1 cut(s) 196
AjnI CCWGG 1 cut(s) 440
AluBI AGCT 2 cut(s) 368, 613
AluI AGCT 2 cut(s) 368, 613
Alw21I GWGCWC 1 cut(s) 967
Alw26I GTCTC 1 cut(s) 835
AlwI GGATC 4 cut(s) 746, 751, 770, 783
AlwNI CAGNNNCTG 1 cut(s) 526
AoxI GGCC 3 cut(s) 6, 925, 969
Asp700I GAANNNNTTC 1 cut(s) 527
AspLEI GCGC 2 cut(s) 565, 896
AspS9I GGNCC 2 cut(s) 868, 925
AsuC2I CCSGG 1 cut(s) 768
AsuHPI GGTGA 1 cut(s) 551
AvaII GGWCC 1 cut(s) 868
BaeGI GKGCMC 1 cut(s) 143
BamHI GGATCC 1 cut(s) 775
Bbv12I GWGCWC 1 cut(s) 967
BccI CCATC 4 cut(s) 110, 166, 470, 879
BceAI ACGGC 1 cut(s) 310
BciT130I CCWGG 1 cut(s) 442
BcnI CCSGG 1 cut(s) 768
BcoDI GTCTC 1 cut(s) 835
BfaI CTAG 2 cut(s) 228, 738
BfmI CTRYAG 1 cut(s) 684
BfoI RGCGCY 1 cut(s) 897
BisI GCNGC 2 cut(s) 298, 301
BlsI GCNGC 2 cut(s) 299, 302
Bme1390I CCNGG 2 cut(s) 442, 768
Bme18I GGWCC 1 cut(s) 868
BmgBI CACGTC 1 cut(s) 196
BmgT120I GGNCC 2 cut(s) 868, 925
BmiI GGNNCC 1 cut(s) 777
BmrFI CCNGG 2 cut(s) 442, 768
BmsI GCATC 2 cut(s) 436, 643
Bpu10I CCTNAGC 1 cut(s) 966
BpuMI CCSGG 1 cut(s) 768
BsaAI YACGTR 1 cut(s) 333
BsaJI CCNNGG 2 cut(s) 292, 441
BsaXI ACNNNNNCTCC 4 cut(s) 139, 169, 335, 365
Bsc4I CCNNNNNNNGG 4 cut(s) 176, 645, 665, 785
Bse1I ACTGG 2 cut(s) 181, 857
BseBI CCWGG 1 cut(s) 442
BseDI CCNNGG 2 cut(s) 292, 441
BseGI GGATG 5 cut(s) 166, 237, 451, 634, 890
BseLI CCNNNNNNNGG 4 cut(s) 176, 645, 665, 785
BseMII CTCAG 5 cut(s) 18, 480, 582, 816, 980
BseNI ACTGG 2 cut(s) 181, 857
BseRI GAGGAG 2 cut(s) 84, 506
BseSI GKGCMC 1 cut(s) 143
BshFI GGCC 3 cut(s) 8, 927, 971
BsiHKAI GWGCWC 1 cut(s) 967
BsiSI CCGG 1 cut(s) 767
BslFI GGGAC 1 cut(s) 389
BslI CCNNNNNNNGG 4 cut(s) 176, 645, 665, 785
BsmAI GTCTC 1 cut(s) 835
BsmFI GGGAC 1 cut(s) 389
BsnI GGCC 3 cut(s) 8, 927, 971
Bsp1286I GDGCHC 2 cut(s) 143, 967
Bsp143I GATC 4 cut(s) 743, 751, 775, 984
BspACI CCGC 5 cut(s) 297, 300, 362, 741, 755
BspANI GGCC 3 cut(s) 8, 927, 971
BspCNI CTCAG 5 cut(s) 19, 481, 583, 817, 979
BspHI TCATGA 1 cut(s) 198
BspLI GGNNCC 1 cut(s) 777
BspMAI CTGCAG 1 cut(s) 688
BspPI GGATC 4 cut(s) 746, 751, 770, 783
BsrI ACTGG 2 cut(s) 181, 857
BssECI CCNNGG 2 cut(s) 292, 441
BssMI GATC 4 cut(s) 743, 751, 775, 984
Bst2UI CCWGG 1 cut(s) 442
Bst4CI ACNGT 4 cut(s) 151, 528, 863, 962
Bst6I CTCTTC 5 cut(s) 47, 52, 265, 282, 328
BstBAI YACGTR 1 cut(s) 333
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 7 cut(s) 27, 355, 489, 591, 825, 872, 966
BstDSI CCRYGG 1 cut(s) 292
BstF5I GGATG 5 cut(s) 166, 237, 451, 634, 890
BstH2I RGCGCY 1 cut(s) 897
BstHHI GCGC 2 cut(s) 565, 896
BstKTI GATC 4 cut(s) 746, 754, 778, 987
BstMAI GTCTC 1 cut(s) 835
BstMBI GATC 4 cut(s) 743, 751, 775, 984
BstMWI GCNNNNNNNGC 2 cut(s) 10, 594
BstNI CCWGG 1 cut(s) 442
BstSCI CCNGG 2 cut(s) 440, 766
BstSFI CTRYAG 1 cut(s) 684
BstSLI GKGCMC 1 cut(s) 143
BstSNI TACGTA 1 cut(s) 333
BstX2I RGATCY 2 cut(s) 743, 775
BstYI RGATCY 2 cut(s) 743, 775
BsuRI GGCC 3 cut(s) 8, 927, 971
BtgI CCRYGG 1 cut(s) 292
BtgZI GCGATG 1 cut(s) 84
BtrI CACGTC 1 cut(s) 196
BtsCI GGATG 5 cut(s) 166, 237, 451, 634, 890
BtsIMutI CAGTG 2 cut(s) 868, 967
Cac8I GCNNGC 1 cut(s) 6
CaiI CAGNNNCTG 1 cut(s) 526
CciI TCATGA 1 cut(s) 198
CfoI GCGC 2 cut(s) 565, 896
Cfr13I GGNCC 2 cut(s) 868, 925
Csp6I GTAC 3 cut(s) 24, 334, 727
CviAII CATG 8 cut(s) 64, 83, 136, 199, 509, 571, 929, 973
CviQI GTAC 3 cut(s) 24, 334, 727
DdeI CTNAG 7 cut(s) 27, 355, 489, 591, 825, 872, 966
DpnI GATC 4 cut(s) 745, 753, 777, 986
DpnII GATC 4 cut(s) 743, 751, 775, 984
DraI TTTAAA 1 cut(s) 103
Eam1104I CTCTTC 5 cut(s) 47, 52, 265, 282, 328
EarI CTCTTC 5 cut(s) 47, 52, 265, 282, 328
Eco105I TACGTA 1 cut(s) 333
Eco147I AGGCCT 1 cut(s) 8
Eco47I GGWCC 1 cut(s) 868
EcoO109I RGGNCCY 1 cut(s) 868
EcoRII CCWGG 1 cut(s) 440
FaeI CATG 8 cut(s) 67, 86, 139, 202, 512, 574, 932, 976
FaqI GGGAC 1 cut(s) 389
FatI CATG 8 cut(s) 63, 82, 135, 198, 508, 570, 928, 972
Fnu4HI GCNGC 2 cut(s) 298, 301
FokI GGATG 5 cut(s) 153, 224, 458, 621, 897
Fsp4HI GCNGC 2 cut(s) 298, 301
FspBI CTAG 2 cut(s) 228, 738
GlaI GCGC 2 cut(s) 564, 895
GluI GCNGC 2 cut(s) 298, 301
HaeII RGCGCY 1 cut(s) 897
HaeIII GGCC 3 cut(s) 8, 927, 971
HapII CCGG 1 cut(s) 767
HhaI GCGC 2 cut(s) 565, 896
Hin1II CATG 8 cut(s) 67, 86, 139, 202, 512, 574, 932, 976
Hin6I GCGC 2 cut(s) 563, 894
HinP1I GCGC 2 cut(s) 563, 894
HincII GTYRAC 2 cut(s) 391, 675
HindII GTYRAC 2 cut(s) 391, 675
HinfI GANTC 7 cut(s) 55, 132, 185, 402, 437, 671, 707
HpaII CCGG 1 cut(s) 767
HphI GGTGA 1 cut(s) 551
Hpy166II GTNNAC 4 cut(s) 286, 391, 675, 727
Hpy188I TCNGA 5 cut(s) 475, 490, 592, 748, 780
Hpy188III TCNNGA 2 cut(s) 199, 704
Hpy8I GTNNAC 4 cut(s) 286, 391, 675, 727
HpyCH4III ACNGT 4 cut(s) 151, 528, 863, 962
HpyCH4IV ACGT 2 cut(s) 195, 332
HpyCH4V TGCA 5 cut(s) 4, 512, 520, 634, 686
HpyF10VI GCNNNNNNNGC 2 cut(s) 10, 594
HpyF3I CTNAG 7 cut(s) 27, 355, 489, 591, 825, 872, 966
HpySE526I ACGT 2 cut(s) 195, 332
Hsp92II CATG 8 cut(s) 67, 86, 139, 202, 512, 574, 932, 976
HspAI GCGC 2 cut(s) 563, 894
Kzo9I GATC 4 cut(s) 743, 751, 775, 984
LweI GCATC 2 cut(s) 436, 643
MaeI CTAG 2 cut(s) 228, 738
MaeII ACGT 2 cut(s) 195, 332
MaeIII GTNAC 1 cut(s) 956
MalI GATC 4 cut(s) 745, 753, 777, 986
MboI GATC 4 cut(s) 743, 751, 775, 984
MboII GAAGA 6 cut(s) 34, 39, 252, 269, 315, 523
MfeI CAATTG 1 cut(s) 660
MflI RGATCY 2 cut(s) 743, 775
MhlI GDGCHC 2 cut(s) 143, 967
MluCI AATT 4 cut(s) 123, 660, 782, 1000
MlyI GAGTC 3 cut(s) 126, 411, 680
MmeI TCCRAC 1 cut(s) 974
MroXI GAANNNNTTC 1 cut(s) 527
MseI TTAA 2 cut(s) 102, 609
MslI CAYNNNNRTG 2 cut(s) 474, 575
MspI CCGG 1 cut(s) 767
MspR9I CCNGG 2 cut(s) 442, 768
MunI CAATTG 1 cut(s) 660
MvaI CCWGG 1 cut(s) 442
MwoI GCNNNNNNNGC 2 cut(s) 10, 594
NciI CCSGG 1 cut(s) 768
NdeII GATC 4 cut(s) 743, 751, 775, 984
NlaIII CATG 8 cut(s) 67, 86, 139, 202, 512, 574, 932, 976
NlaIV GGNNCC 1 cut(s) 777
NmuCI GTSAC 1 cut(s) 956
PagI TCATGA 1 cut(s) 198
PceI AGGCCT 1 cut(s) 8
PcsI WCGNNNNNNNCGW 1 cut(s) 127
PdmI GAANNNNTTC 1 cut(s) 527
PfeI GAWTC 4 cut(s) 55, 185, 437, 707
PkrI GCNGC 2 cut(s) 299, 302
PleI GAGTC 3 cut(s) 126, 410, 679
PpsI GAGTC 3 cut(s) 126, 410, 679
Ppu21I YACGTR 1 cut(s) 333
PpuMI RGGWCCY 1 cut(s) 868
Psp5II RGGWCCY 1 cut(s) 868
Psp6I CCWGG 1 cut(s) 440
PspGI CCWGG 1 cut(s) 440
PspN4I GGNNCC 1 cut(s) 777
PspPI GGNCC 2 cut(s) 868, 925
PspPPI RGGWCCY 1 cut(s) 868
PstI CTGCAG 1 cut(s) 688
PstNI CAGNNNCTG 1 cut(s) 526
PsuI RGATCY 2 cut(s) 743, 775
RsaI GTAC 3 cut(s) 25, 335, 728
RsaNI GTAC 3 cut(s) 24, 334, 727
RseI CAYNNNNRTG 2 cut(s) 474, 575
SaqAI TTAA 2 cut(s) 102, 609
SatI GCNGC 2 cut(s) 298, 301
Sau3AI GATC 4 cut(s) 743, 751, 775, 984
Sau96I GGNCC 2 cut(s) 868, 925
SchI GAGTC 3 cut(s) 126, 411, 680
ScrFI CCNGG 2 cut(s) 442, 768
SduI GDGCHC 2 cut(s) 143, 967
SetI ASST 8 cut(s) 198, 260, 335, 348, 370, 615, 870, 906
SfaNI GCATC 2 cut(s) 436, 643
SfcI CTRYAG 1 cut(s) 684
SinI GGWCC 1 cut(s) 868
SmiMI CAYNNNNRTG 2 cut(s) 474, 575
SnaBI TACGTA 1 cut(s) 333
Sse9I AATT 4 cut(s) 123, 660, 782, 1000
SseBI AGGCCT 1 cut(s) 8
SsiI CCGC 5 cut(s) 297, 300, 362, 741, 755
SspMI CTAG 2 cut(s) 228, 738
StuI AGGCCT 1 cut(s) 8
StyD4I CCNGG 2 cut(s) 440, 766
TaaI ACNGT 4 cut(s) 151, 528, 863, 962
TaiI ACGT 2 cut(s) 198, 335
TaqI TCGA 2 cut(s) 121, 183
TasI AATT 4 cut(s) 123, 660, 782, 1000
TauI GCSGC 2 cut(s) 300, 303
TfiI GAWTC 4 cut(s) 55, 185, 437, 707
Tru1I TTAA 2 cut(s) 102, 609
Tru9I TTAA 2 cut(s) 102, 609
TscAI CASTG 2 cut(s) 868, 967
TseFI GTSAC 1 cut(s) 956
Tsp45I GTSAC 1 cut(s) 956
TspDTI ATGAA 2 cut(s) 155, 985
TspRI CASTG 2 cut(s) 868, 967
VpaK11BI GGWCC 1 cut(s) 868
XmnI GAANNNNTTC 1 cut(s) 527
XspI CTAG 2 cut(s) 228, 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.