Rw6G008300

Carboxylesterase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000397
Physical Location & Seq
Forward (+)
14102701 .. 14104518
1818 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G008300.1

Sequence Viewer

Length: 954 bp
ATGAGCGACGAACTAGCCCATGATTTCTCTCCCATGATCAAAGTATACAAAGACGGTCGAATCGACCGACCCTGGGGAACAACCACAGTCCCTGCATCCATCGATCCCCAAACCGGTGTTCAATCCAAAGACGTCGTCATCTCAACCGCACCACCCGTCTCTGTAAGGCTTTACATTCCCAAATCCACCACAACCGAGTCAGCCGAGTCAACCCGGAAGCTCCCTCTTCTGGTTTACTTTCACGGCGGTGCCTTCTGCATTGGAAGTGCTTTCTCTCGCATATGTCACAACCACTGCAACTCTTTAGTCTCTGAAGCCAATGTTGTAGCTGTTTCTGTTGACTATAGGTTGGTGCCGGAGCACCCTCTTCCGGCTGCCTATGAAGATTCATGGGCCGCTCTCAAATGGGTGGAGTCCCATTTCGCCGGAACCGGCCCGGAAGAGTGGCTGAACCGGCATGCAGATTTGAACCGGGTTTTCTTTTCAGGTGATAGCGCTGGGGCAAACATAGCTCACCACATGGGTTTGAGAGTTGGGTCTGAGAAATTGGTGTATTTTAAGCTGAATGGGATTGTTCTGGTGCATCCATACTTTTGGGGTGAAGAACCAGTAGGCGGAGAGGTAGCTTTGGGCGAGAGTCATAGAGAGCATTTGCATGCTTTGTGGAGGTTTGCTTACCCTTTGACTAGTGGATCTGATGACCCGCTTTTTAATCCGGGTAAGGATCCGAAGTTGGGGGAGTTGGGTTGTGAGAAAGTGTTGGTCTGTGTTGCGGAGAAGGATGCGTTGAAAGATAGGGGGTGGTATTACAGTGAGGTGTTGAAGAAGAGTGGATGGAATGGAGTAGTAGAGGTTTTGGAAACAAAGGAGGAGCAGCATGTGTTCCATTTGATCAATCCTAGTTGTGACAATGCTGTGACCATGCTCAAGAAGATTGTTTCTTTCATGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

317

Amino Acids

35.14

Weight (kDa)

5.91

Isoelectric Point (pI)

27.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
COesterase PF00135 57 - 180 1.7e-09 Carboxylesterase family
BD-FAE PF20434 69 - 181 1.7e-12 BD-FAE
Abhydrolase_3 PF07859 77 - 296 4.6e-54 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000397)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G48690 AT3G48700
fragaria_vesca FvH4_2g06400 FvH4_2g06430 FvH4_2g06450 FvH4_2g06480 FvH4_2g06560
malus_domestica MD05G1076400.v1.1 MD05G1076500.v1.1 MD05G1078900.v1.1 MD05G1191000.v1.1 MD05G1191100.v1.1 MD08G1226300.v1.1 MD10G1091000.v1.1 MD10G1091200.v1.1 MD10G1091400.v1.1 MD10G1091600.v1.1 MD10G1091900.v1.1
prunus_persica Prupe.8G120800_v2.0.a1 Prupe.8G121100_v2.0.a1 Prupe.8G121200_v2.0.a1 Prupe.8G121300_v2.0.a1 Prupe.8G121400_v2.0.a1 Prupe.8G121500_v2.0.a1 Prupe.8G121600_v2.0.a1 Prupe.8G121700_v2.0.a1 Prupe.8G121900_v2.0.a1 Prupe.8G122000_v2.0.a1 Prupe.I000800_v2.0.a1
pyrus_communis pycom05g07040 pycom05g17560 pycom05g17680 pycom05g17760 pycom10g07450 pycom10g07470 pycom10g07550
rosa_chinensis RchiOBHm_Chr6g0258271 RchiOBHm_Chr6g0258281 RchiOBHm_Chr6g0258291 RchiOBHm_Chr6g0258311 RchiOBHm_Chr6g0258321 RchiOBHm_Chr6g0258501
rosa_laevigata RLG00000014606 RLG00000014621 RLG00000014622 RLG00000014624 RLG00000014625 RLG00000014627 RLG00000014629 RLG00000014633 RLG00000014635
rosa_multiflora Rmu_co8014592.1_g000001 Rmu_co8366243.1_g000001 Rmu_sc0001663.1_g000004 Rmu_sc0005877.1_g000001 Rmu_sc0005877.1_g000006 Rmu_sc0005877.1_g000007 Rmu_sc0007367.1_g000009 Rmu_sc0007367.1_g000019 Rmu_sc0010616.1_g000022 Rmu_sc0010616.1_g000023 Rmu_ssc0000213.1_g000009
rosa_roxburghii Rroxscaffold_178G00437230 Rroxscaffold_178G00437250 Rroxscaffold_178G00437280 Rroxscaffold_178G00437320 Rroxscaffold_178G00437340 Rroxscaffold_178G00437350 Rroxscaffold_178G00437480 Rroxscaffold_7G00205390 Rroxscaffold_7G00205410 Rroxscaffold_7G00205450 Rroxscaffold_7G00205490 Rroxscaffold_7G00205510 Rroxscaffold_7G00205520 Rroxscaffold_7G00205670 Rroxscaffold_7G00205730
rosa_rugosa Rorug05G0579800 Rorug05G0580500 Rorug05G0580600 Rorug05G0580700 Rorug05G0580900 Rorug05G0581000 Rorug05G0581900
rosa_samantha Rh6CG084800 Rh6CG085100 Rh6CG085200 Rh6CG085400 Rh6CG085500 Rh6CG085600 Rh6CG085700 Rh6CG086900
rosa_wichuraiana Rw6G008300 Rw6G008310 Rw6G008360 Rw6G008370 Rw6G008380 Rw6G008390 Rw6G008430 Rw6G008440 Rw6G008530

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 135
AccB1I GGYRCC 2 cut(s) 248, 352
AccBSI CCGCTC 1 cut(s) 398
AccI GTMKAC 1 cut(s) 45
AciI CCGC 6 cut(s) 147, 246, 396, 615, 704, 773
AclWI GGATC 4 cut(s) 98, 700, 719, 732
AcuI CTGAAG 1 cut(s) 333
AcyI GRCGYC 1 cut(s) 132
AfeI AGCGCT 1 cut(s) 496
AfiI CCNNNNNNNGG 6 cut(s) 73, 113, 229, 370, 614, 734
AgeI ACCGGT 1 cut(s) 113
AgsI TTSAA 4 cut(s) 122, 469, 790, 823
AhlI ACTAGT 1 cut(s) 686
AjnI CCWGG 1 cut(s) 71
AjuI GAANNNNNNNTTGG 2 cut(s) 102, 134
AleI CACNNNNGTG 1 cut(s) 246
AluBI AGCT 5 cut(s) 220, 329, 512, 562, 626
AluI AGCT 5 cut(s) 220, 329, 512, 562, 626
Alw21I GWGCWC 1 cut(s) 363
Alw26I GTCTC 2 cut(s) 163, 313
AlwI GGATC 4 cut(s) 98, 700, 719, 732
AlwNI CAGNNNCTG 1 cut(s) 92
Aor51HI AGCGCT 1 cut(s) 496
AoxI GGCC 2 cut(s) 393, 433
ApeKI GCWGC 2 cut(s) 374, 874
ArsI GACNNNNNNTTYG 2 cut(s) 120, 152
AsiGI ACCGGT 1 cut(s) 113
AspLEI GCGC 1 cut(s) 497
AspS9I GGNCC 2 cut(s) 393, 434
AsuC2I CCSGG 4 cut(s) 214, 437, 473, 717
AsuHPI GGTGA 3 cut(s) 500, 506, 611
BamHI GGATCC 1 cut(s) 724
BanI GGYRCC 2 cut(s) 248, 352
Bbv12I GWGCWC 1 cut(s) 363
BbvI GCAGC 2 cut(s) 361, 886
BccI CCATC 2 cut(s) 107, 828
BceAI ACGGC 1 cut(s) 259
BciT130I CCWGG 1 cut(s) 73
BclI TGATCA 2 cut(s) 36, 891
BcnI CCSGG 4 cut(s) 214, 437, 473, 717
BcoDI GTCTC 2 cut(s) 163, 313
BcuI ACTAGT 1 cut(s) 686
BfaI CTAG 3 cut(s) 14, 687, 900
BfmI CTRYAG 1 cut(s) 343
BfoI RGCGCY 1 cut(s) 498
BisI GCNGC 3 cut(s) 375, 396, 875
BlsI GCNGC 3 cut(s) 376, 397, 876
Bme1390I CCNGG 5 cut(s) 73, 214, 437, 473, 717
BmgT120I GGNCC 2 cut(s) 393, 434
BmiI GGNNCC 4 cut(s) 250, 354, 430, 726
BmrFI CCNGG 5 cut(s) 73, 214, 437, 473, 717
BmsI GCATC 3 cut(s) 104, 592, 772
BpuEI CTTGAG 1 cut(s) 911
BpuMI CCSGG 4 cut(s) 214, 437, 473, 717
Bsa29I ATCGAT 1 cut(s) 102
BsaHI GRCGYC 1 cut(s) 132
BsaJI CCNNGG 2 cut(s) 71, 72
BsaWI WCCGGW 1 cut(s) 113
Bsc4I CCNNNNNNNGG 6 cut(s) 73, 113, 229, 370, 614, 734
Bse118I RCCGGY 3 cut(s) 113, 431, 453
Bse1I ACTGG 1 cut(s) 608
BseBI CCWGG 1 cut(s) 73
BseCI ATCGAT 1 cut(s) 102
BseDI CCNNGG 2 cut(s) 71, 72
BseGI GGATG 4 cut(s) 95, 583, 787, 839
BseLI CCNNNNNNNGG 6 cut(s) 73, 113, 229, 370, 614, 734
BseMII CTCAG 1 cut(s) 531
BseNI ACTGG 1 cut(s) 608
BseRI GAGGAG 1 cut(s) 884
BseXI GCAGC 2 cut(s) 361, 886
BseYI CCCAGC 1 cut(s) 497
Bsh1285I CGRYCG 2 cut(s) 58, 67
BshFI GGCC 2 cut(s) 395, 435
BshNI GGYRCC 2 cut(s) 248, 352
BshTI ACCGGT 1 cut(s) 113
BshVI ATCGAT 1 cut(s) 102
BsiEI CGRYCG 2 cut(s) 58, 67
BsiHKAI GWGCWC 1 cut(s) 363
BslFI GGGAC 2 cut(s) 74, 400
BslI CCNNNNNNNGG 6 cut(s) 73, 113, 229, 370, 614, 734
BsmAI GTCTC 2 cut(s) 163, 313
BsmBI CGTCTC 1 cut(s) 163
BsmFI GGGAC 2 cut(s) 74, 400
BsnI GGCC 2 cut(s) 395, 435
Bsp1286I GDGCHC 1 cut(s) 363
Bsp143I GATC 5 cut(s) 36, 103, 692, 724, 891
BspACI CCGC 6 cut(s) 147, 246, 396, 615, 704, 773
BspANI GGCC 2 cut(s) 395, 435
BspCNI CTCAG 1 cut(s) 532
BspDI ATCGAT 1 cut(s) 102
BspHI TCATGA 1 cut(s) 945
BspLI GGNNCC 4 cut(s) 250, 354, 430, 726
BspPI GGATC 4 cut(s) 98, 700, 719, 732
BspT107I GGYRCC 2 cut(s) 248, 352
BsrBI CCGCTC 1 cut(s) 398
BsrFI RCCGGY 3 cut(s) 113, 431, 453
BsrI ACTGG 1 cut(s) 608
BssAI RCCGGY 3 cut(s) 113, 431, 453
BssECI CCNNGG 2 cut(s) 71, 72
BssMI GATC 5 cut(s) 36, 103, 692, 724, 891
BssNAI GTATAC 1 cut(s) 46
BssNI GRCGYC 1 cut(s) 132
Bst1107I GTATAC 1 cut(s) 46
Bst2UI CCWGG 1 cut(s) 73
Bst4CI ACNGT 3 cut(s) 56, 88, 812
Bst6I CTCTTC 4 cut(s) 231, 372, 435, 821
BstACI GRCGYC 1 cut(s) 132
BstC8I GCNNGC 2 cut(s) 459, 657
BstDEI CTNAG 1 cut(s) 540
BstF5I GGATG 4 cut(s) 95, 583, 787, 839
BstH2I RGCGCY 1 cut(s) 498
BstHHI GCGC 1 cut(s) 497
BstKTI GATC 5 cut(s) 39, 106, 695, 727, 894
BstMAI GTCTC 2 cut(s) 163, 313
BstMBI GATC 5 cut(s) 36, 103, 692, 724, 891
BstMCI CGRYCG 2 cut(s) 58, 67
BstMWI GCNNNNNNNGC 2 cut(s) 454, 509
BstNI CCWGG 1 cut(s) 73
BstNSI RCATGY 3 cut(s) 461, 659, 881
BstSCI CCNGG 5 cut(s) 71, 212, 435, 471, 715
BstSFI CTRYAG 1 cut(s) 343
BstV1I GCAGC 2 cut(s) 361, 886
BstX2I RGATCY 2 cut(s) 692, 724
BstXI CCANNNNNNTGG 1 cut(s) 594
BstYI RGATCY 2 cut(s) 692, 724
BstZ17I GTATAC 1 cut(s) 46
Bsu15I ATCGAT 1 cut(s) 102
BsuRI GGCC 2 cut(s) 395, 435
BsuTUI ATCGAT 1 cut(s) 102
BtsCI GGATG 4 cut(s) 95, 583, 787, 839
BtsI GCAGTG 1 cut(s) 292
BtsIMutI CAGTG 2 cut(s) 292, 817
Cac8I GCNNGC 2 cut(s) 459, 657
CaiI CAGNNNCTG 1 cut(s) 92
CciI TCATGA 1 cut(s) 945
CfoI GCGC 1 cut(s) 497
Cfr10I RCCGGY 3 cut(s) 113, 431, 453
Cfr13I GGNCC 2 cut(s) 393, 434
ClaI ATCGAT 1 cut(s) 102
CspAI ACCGGT 1 cut(s) 113
CviAII CATG 9 cut(s) 20, 34, 390, 458, 520, 656, 878, 922, 946
DdeI CTNAG 1 cut(s) 540
DpnI GATC 5 cut(s) 38, 105, 694, 726, 893
DpnII GATC 5 cut(s) 36, 103, 692, 724, 891
Eam1104I CTCTTC 4 cut(s) 231, 372, 435, 821
EarI CTCTTC 4 cut(s) 231, 372, 435, 821
EciI GGCGGA 1 cut(s) 630
Eco47III AGCGCT 1 cut(s) 496
Eco57I CTGAAG 1 cut(s) 333
EcoRII CCWGG 1 cut(s) 71
Esp3I CGTCTC 1 cut(s) 163
FaeI CATG 9 cut(s) 23, 37, 393, 461, 523, 659, 881, 925, 949
FaqI GGGAC 2 cut(s) 74, 400
FatI CATG 9 cut(s) 19, 33, 389, 457, 519, 655, 877, 921, 945
FauI CCCGC 1 cut(s) 711
FauNDI CATATG 1 cut(s) 281
FbaI TGATCA 2 cut(s) 36, 891
FblI GTMKAC 1 cut(s) 45
Fnu4HI GCNGC 3 cut(s) 375, 396, 875
FokI GGATG 4 cut(s) 82, 570, 794, 846
Fsp4HI GCNGC 3 cut(s) 375, 396, 875
FspBI CTAG 3 cut(s) 14, 687, 900
GlaI GCGC 1 cut(s) 496
GluI GCNGC 3 cut(s) 375, 396, 875
GsaI CCCAGC 1 cut(s) 501
HaeII RGCGCY 1 cut(s) 498
HaeIII GGCC 2 cut(s) 395, 435
HhaI GCGC 1 cut(s) 497
Hin1I GRCGYC 1 cut(s) 132
Hin1II CATG 9 cut(s) 23, 37, 393, 461, 523, 659, 881, 925, 949
Hin6I GCGC 1 cut(s) 495
HinP1I GCGC 1 cut(s) 495
HincII GTYRAC 2 cut(s) 210, 340
HindII GTYRAC 2 cut(s) 210, 340
HinfI GANTC 6 cut(s) 60, 197, 206, 386, 413, 637
HphI GGTGA 3 cut(s) 500, 506, 611
Hpy166II GTNNAC 4 cut(s) 46, 210, 235, 340
Hpy188I TCNGA 4 cut(s) 313, 541, 697, 729
Hpy188III TCNNGA 2 cut(s) 928, 946
Hpy8I GTNNAC 4 cut(s) 46, 210, 235, 340
Hpy99I CGWCG 2 cut(s) 11, 137
HpyAV CCTTC 2 cut(s) 262, 772
HpyCH4III ACNGT 3 cut(s) 56, 88, 812
HpyCH4IV ACGT 1 cut(s) 132
HpyCH4V TGCA 6 cut(s) 95, 258, 297, 461, 583, 655
HpyF10VI GCNNNNNNNGC 2 cut(s) 454, 509
HpyF3I CTNAG 1 cut(s) 540
HpySE526I ACGT 1 cut(s) 132
Hsp92I GRCGYC 1 cut(s) 132
Hsp92II CATG 9 cut(s) 23, 37, 393, 461, 523, 659, 881, 925, 949
HspAI GCGC 1 cut(s) 495
Ksp22I TGATCA 2 cut(s) 36, 891
Kzo9I GATC 5 cut(s) 36, 103, 692, 724, 891
LmnI GCTCC 3 cut(s) 225, 358, 871
Lsp1109I GCAGC 2 cut(s) 361, 886
LweI GCATC 3 cut(s) 104, 592, 772
MaeI CTAG 3 cut(s) 14, 687, 900
MaeII ACGT 1 cut(s) 132
MaeIII GTNAC 3 cut(s) 284, 905, 916
MalI GATC 5 cut(s) 38, 105, 694, 726, 893
MbiI CCGCTC 1 cut(s) 398
MboI GATC 5 cut(s) 36, 103, 692, 724, 891
MboII GAAGA 8 cut(s) 218, 359, 395, 452, 614, 835, 838, 943
MflI RGATCY 2 cut(s) 692, 724
MhlI GDGCHC 1 cut(s) 363
MluCI AATT 2 cut(s) 545, 949
MlyI GAGTC 4 cut(s) 206, 215, 422, 646
MnlI CCTC 7 cut(s) 234, 375, 613, 660, 808, 844, 862
MseI TTAA 2 cut(s) 558, 711
MslI CAYNNNNRTG 2 cut(s) 246, 654
MspR9I CCNGG 5 cut(s) 73, 214, 437, 473, 717
MvaI CCWGG 1 cut(s) 73
MwoI GCNNNNNNNGC 2 cut(s) 454, 509
NciI CCSGG 4 cut(s) 214, 437, 473, 717
NdeI CATATG 1 cut(s) 281
NdeII GATC 5 cut(s) 36, 103, 692, 724, 891
NlaIII CATG 9 cut(s) 23, 37, 393, 461, 523, 659, 881, 925, 949
NlaIV GGNNCC 4 cut(s) 250, 354, 430, 726
NmeAIII GCCGAG 1 cut(s) 229
NmuCI GTSAC 3 cut(s) 284, 905, 916
NspI RCATGY 3 cut(s) 461, 659, 881
OliI CACNNNNGTG 1 cut(s) 246
PaeI GCATGC 2 cut(s) 461, 659
PagI TCATGA 1 cut(s) 945
PasI CCCWGGG 1 cut(s) 72
PcsI WCGNNNNNNNCGW 2 cut(s) 60, 64
PfeI GAWTC 2 cut(s) 60, 386
PflFI GACNNNGTC 1 cut(s) 134
PinAI ACCGGT 1 cut(s) 113
PkrI GCNGC 3 cut(s) 376, 397, 876
PleI GAGTC 4 cut(s) 205, 214, 421, 645
PpsI GAGTC 4 cut(s) 205, 214, 421, 645
Psp6I CCWGG 1 cut(s) 71
PspFI CCCAGC 1 cut(s) 497
PspGI CCWGG 1 cut(s) 71
PspN4I GGNNCC 4 cut(s) 250, 354, 430, 726
PspPI GGNCC 2 cut(s) 393, 434
PstNI CAGNNNCTG 1 cut(s) 92
PsuI RGATCY 2 cut(s) 692, 724
PsyI GACNNNGTC 1 cut(s) 134
RseI CAYNNNNRTG 2 cut(s) 246, 654
SaqAI TTAA 2 cut(s) 558, 711
SatI GCNGC 3 cut(s) 375, 396, 875
Sau3AI GATC 5 cut(s) 36, 103, 692, 724, 891
Sau96I GGNCC 2 cut(s) 393, 434
SchI GAGTC 4 cut(s) 206, 215, 422, 646
ScrFI CCNGG 5 cut(s) 73, 214, 437, 473, 717
SduI GDGCHC 1 cut(s) 363
SfaNI GCATC 3 cut(s) 104, 592, 772
SfcI CTRYAG 1 cut(s) 343
SmiMI CAYNNNNRTG 2 cut(s) 246, 654
SmlI CTYRAG 1 cut(s) 926
SmoI CTYRAG 1 cut(s) 926
SpeI ACTAGT 1 cut(s) 686
SphI GCATGC 2 cut(s) 461, 659
Sse9I AATT 2 cut(s) 545, 949
SsiI CCGC 6 cut(s) 147, 246, 396, 615, 704, 773
SspMI CTAG 3 cut(s) 14, 687, 900
StyD4I CCNGG 5 cut(s) 71, 212, 435, 471, 715
TaaI ACNGT 3 cut(s) 56, 88, 812
TaiI ACGT 1 cut(s) 135
TaqI TCGA 3 cut(s) 58, 63, 102
TaqII GACCGA 1 cut(s) 81
TasI AATT 2 cut(s) 545, 949
TauI GCSGC 1 cut(s) 398
TfiI GAWTC 2 cut(s) 60, 386
Tru1I TTAA 2 cut(s) 558, 711
Tru9I TTAA 2 cut(s) 558, 711
TscAI CASTG 2 cut(s) 299, 817
TseFI GTSAC 3 cut(s) 284, 905, 916
TseI GCWGC 2 cut(s) 374, 874
Tsp45I GTSAC 3 cut(s) 284, 905, 916
TspDTI ATGAA 3 cut(s) 378, 396, 934
TspRI CASTG 2 cut(s) 299, 817
Tth111I GACNNNGTC 1 cut(s) 134
XceI RCATGY 3 cut(s) 461, 659, 881
XmiI GTMKAC 1 cut(s) 45
XspI CTAG 3 cut(s) 14, 687, 900
ZraI GACGTC 1 cut(s) 133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.